AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594140 MtrunA17_Chr4g0012941 87.179 39 4 1 1 38 41 79 3.02e-18 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTGCTTCACAACAATA+TGG 0.222130 4_2:+21238581 None:intergenic
GAATACCACATAAGGCAAAT+AGG 0.312154 4_2:+21238450 None:intergenic
ATTCGTTGCATGTATCGTTT+TGG 0.351230 4_2:-21238714 Msa0594140:five_prime_UTR
AATCTATCAGCTTCTGCAAT+AGG 0.389891 4_2:+21238671 None:intergenic
AGTAAGAGTATAAATAATAA+AGG 0.397290 4_2:+21238646 None:intergenic
AATACCACATAAGGCAAATA+GGG 0.405661 4_2:+21238451 None:intergenic
GAAATATAACCTTGAAGAAA+AGG 0.420263 4_2:+21238614 None:intergenic
TGGTTATATTTACAGAATCA+AGG 0.425590 4_2:-21235959 Msa0594140:intron
AGTTGATATCGCGAGTAATC+TGG 0.431895 4_2:+21238417 None:intergenic
GTGGAAGTTGTGGCGGATGT+TGG 0.448150 4_2:-21235934 Msa0594140:CDS
TCACTGCTTCACAACAATAT+GGG 0.474158 4_2:+21238582 None:intergenic
TTGTGACTGCTAGAGAGATA+TGG 0.476164 4_2:-21238513 Msa0594140:exon
GCTGCCCTATTTGCCTTATG+TGG 0.498988 4_2:-21238455 Msa0594140:CDS
GGTTATATTTACAGAATCAA+GGG 0.515885 4_2:-21235958 Msa0594140:intron
GGGTGTTTGAATACCACATA+AGG 0.517200 4_2:+21238442 None:intergenic
AAATTAAGCACTCTATATAG+TGG 0.524276 4_2:+21235894 None:intergenic
CCTTGAAGAAAAGGATAGCA+TGG 0.525784 4_2:+21238623 None:intergenic
TTCTTTAAAGACTTTGAATG+TGG 0.531694 4_2:-21238486 Msa0594140:CDS
AAGACTTTGAATGTGGAGAA+TGG 0.533346 4_2:-21238479 Msa0594140:CDS
AATCAAGGGTGTGGAAGTTG+TGG 0.551229 4_2:-21235944 Msa0594140:CDS
TATTTACAGAATCAAGGGTG+TGG 0.556405 4_2:-21235953 Msa0594140:intron
TTGTGGCGGATGTTGGATCA+AGG 0.564536 4_2:-21235927 Msa0594140:CDS
GATATCGCGAGTAATCTGGT+AGG 0.618889 4_2:+21238421 None:intergenic
ATATCGCGAGTAATCTGGTA+GGG 0.629903 4_2:+21238422 None:intergenic
TAGACGATCATCATAGTCGA+TGG 0.640137 4_2:+21238541 None:intergenic
CAAGGGTGTGGAAGTTGTGG+CGG 0.655854 4_2:-21235941 Msa0594140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATATAATATAATCTATT+TGG - chr4_2:21236315-21236334 Msa0594140:intron 10.0%
!! TAAAATAAACAATTTAGTTA+AGG - chr4_2:21237128-21237147 Msa0594140:intron 10.0%
!!! TTTATCTAAATTCATTTTTA+AGG + chr4_2:21237287-21237306 None:intergenic 10.0%
!! AAAATATAGTAAAATATATC+AGG + chr4_2:21237403-21237422 None:intergenic 10.0%
!!! TTTTTTTTAACAAACAAAAA+TGG + chr4_2:21238118-21238137 None:intergenic 10.0%
!!! AGTAAGAGTATAAATAATAA+AGG + chr4_2:21235971-21235990 None:intergenic 15.0%
!!! TTGATTTTTATTTTCTTTTC+TGG - chr4_2:21236793-21236812 Msa0594140:intron 15.0%
!!! TGATTTTTATTTTCTTTTCT+GGG - chr4_2:21236794-21236813 Msa0594140:intron 15.0%
!! AAAATTAATAAAGTATATGG+TGG - chr4_2:21236978-21236997 Msa0594140:intron 15.0%
!! TAACCTTAAATATCTAATAT+GGG + chr4_2:21237021-21237040 None:intergenic 15.0%
!! TTAACCTTAAATATCTAATA+TGG + chr4_2:21237022-21237041 None:intergenic 15.0%
!! TAATTTGACAGAAAATATAT+TGG - chr4_2:21237222-21237241 Msa0594140:intron 15.0%
!! TAAAATATATCAGGAAATAA+CGG + chr4_2:21237394-21237413 None:intergenic 15.0%
!! CTAAATTATCCTTAAAAAAA+AGG + chr4_2:21237472-21237491 None:intergenic 15.0%
!!! GTTCAAATATTTTTCATTTA+AGG + chr4_2:21237681-21237700 None:intergenic 15.0%
!!! TACTTTTGTTGATTATTTAA+TGG - chr4_2:21238401-21238420 Msa0594140:intron 15.0%
!!! ATAAATTTCTTATTTACCTT+TGG + chr4_2:21238619-21238638 None:intergenic 15.0%
!!! TTATTAATGACTTCTTTTAT+TGG - chr4_2:21238635-21238654 Msa0594140:five_prime_UTR 15.0%
!! TCAATGTTATGTTATTGTTA+TGG + chr4_2:21236272-21236291 None:intergenic 20.0%
!! AATCTCGTTTATATATTAGT+TGG - chr4_2:21236524-21236543 Msa0594140:intron 20.0%
!!! AGATTGTTGATGATTATTTT+TGG - chr4_2:21236747-21236766 Msa0594140:intron 20.0%
!!! ATTTAAGGTTAAAGTGTTAA+CGG - chr4_2:21237030-21237049 Msa0594140:intron 20.0%
!!! TGAGAAGTTTTTTTTAGTTA+AGG - chr4_2:21237097-21237116 Msa0594140:intron 20.0%
!!! GAGAAGTTTTTTTTAGTTAA+GGG - chr4_2:21237098-21237117 Msa0594140:intron 20.0%
!! ATTATGCCTAAAAAATTTGA+AGG - chr4_2:21237163-21237182 Msa0594140:intron 20.0%
!!! TCATTTTTAAGGATTTGATA+GGG + chr4_2:21237276-21237295 None:intergenic 20.0%
!!! TTCATTTTTAAGGATTTGAT+AGG + chr4_2:21237277-21237296 None:intergenic 20.0%
!! ATACCAATATTGCAATTAAT+CGG - chr4_2:21237308-21237327 Msa0594140:intron 20.0%
!! AAAATATATCAGGAAATAAC+GGG + chr4_2:21237393-21237412 None:intergenic 20.0%
!!! TTCTTTCATCCTTTTTTTTA+AGG - chr4_2:21237460-21237479 Msa0594140:intron 20.0%
!!! TGAAATGAGTCAATTATTTT+AGG + chr4_2:21237933-21237952 None:intergenic 20.0%
!! AAATACACTAATATTTGACA+AGG - chr4_2:21238195-21238214 Msa0594140:intron 20.0%
!! AATACACTAATATTTGACAA+GGG - chr4_2:21238196-21238215 Msa0594140:intron 20.0%
!! AAGAAAAATGTATTAGTGAA+GGG + chr4_2:21238244-21238263 None:intergenic 20.0%
!! TAAGAAAAATGTATTAGTGA+AGG + chr4_2:21238245-21238264 None:intergenic 20.0%
!!! ATAAAGCTAGAGTTTTTTTA+GGG + chr4_2:21238542-21238561 None:intergenic 20.0%
!!! CAATTTTGAAAATAAATCCT+AGG + chr4_2:21238575-21238594 None:intergenic 20.0%
!!! TAGTTAATGAATTTTTCCAA+AGG - chr4_2:21238600-21238619 Msa0594140:five_prime_UTR 20.0%
! GAAATATAACCTTGAAGAAA+AGG + chr4_2:21236003-21236022 None:intergenic 25.0%
!! TTCTTTAAAGACTTTGAATG+TGG - chr4_2:21236128-21236147 Msa0594140:intron 25.0%
!! TTAGTGAAAGTATACATACT+TGG + chr4_2:21236226-21236245 None:intergenic 25.0%
! TCGTTTATATATTAGTTGGT+AGG - chr4_2:21236528-21236547 Msa0594140:intron 25.0%
! TAATATAATATGCAGCACAA+CGG + chr4_2:21236697-21236716 None:intergenic 25.0%
!!! TTTTATTTTCTTTTCTGGGT+AGG - chr4_2:21236798-21236817 Msa0594140:intron 25.0%
! GGGAAAAAATTAGATATTGA+TGG - chr4_2:21236879-21236898 Msa0594140:intron 25.0%
!! AATTAGATATTGATGGTTCA+CGG - chr4_2:21236886-21236905 Msa0594140:intron 25.0%
!!! TTTTTTAAAAAGAAGACCGT+TGG + chr4_2:21236936-21236955 None:intergenic 25.0%
!! ATATGGGCTAAAATTAAAAG+TGG + chr4_2:21237005-21237024 None:intergenic 25.0%
! TAGCCCATATTAGATATTTA+AGG - chr4_2:21237015-21237034 Msa0594140:intron 25.0%
! TTTCTTGCCTTTATATGTTA+TGG + chr4_2:21237062-21237081 None:intergenic 25.0%
! CTAAAAAAAACTTCTCAAAG+TGG + chr4_2:21237094-21237113 None:intergenic 25.0%
!!! TTCATGCCTTCAAATTTTTT+AGG + chr4_2:21237172-21237191 None:intergenic 25.0%
! TATCCGATTAATTGCAATAT+TGG + chr4_2:21237314-21237333 None:intergenic 25.0%
! GTCAAGGAGTTATATATTTA+GGG + chr4_2:21237510-21237529 None:intergenic 25.0%
! AGTCAAGGAGTTATATATTT+AGG + chr4_2:21237511-21237530 None:intergenic 25.0%
! TCATTGATAAATACAATGAG+GGG + chr4_2:21237635-21237654 None:intergenic 25.0%
! CTCATTGATAAATACAATGA+GGG + chr4_2:21237636-21237655 None:intergenic 25.0%
! TCTCATTGATAAATACAATG+AGG + chr4_2:21237637-21237656 None:intergenic 25.0%
!!! AATGAGAGAAACTTTTTCAT+GGG - chr4_2:21237650-21237669 Msa0594140:intron 25.0%
!! TGCTCAAAAGTTTTTCATTT+GGG - chr4_2:21237804-21237823 Msa0594140:intron 25.0%
! CCATGAAAGAATTAAAGATA+CGG - chr4_2:21237834-21237853 Msa0594140:intron 25.0%
! AGAAAAATGTATTAGTGAAG+GGG + chr4_2:21238243-21238262 None:intergenic 25.0%
! AAAACTCACTTAAATTGAGA+CGG - chr4_2:21238286-21238305 Msa0594140:intron 25.0%
! AATTCAAAGACAGTCTTTAT+TGG - chr4_2:21238364-21238383 Msa0594140:intron 25.0%
! ATTCAAAGACAGTCTTTATT+GGG - chr4_2:21238365-21238384 Msa0594140:intron 25.0%
!! TTTAAGATTGAGTCACTATT+TGG - chr4_2:21238470-21238489 Msa0594140:CDS 25.0%
!!! TAAAGCTAGAGTTTTTTTAG+GGG + chr4_2:21238541-21238560 None:intergenic 25.0%
!!! GATAAAGCTAGAGTTTTTTT+AGG + chr4_2:21238543-21238562 None:intergenic 25.0%
! TGGTTATATTTACAGAATCA+AGG - chr4_2:21238655-21238674 Msa0594140:five_prime_UTR 25.0%
! GGTTATATTTACAGAATCAA+GGG - chr4_2:21238656-21238675 Msa0594140:five_prime_UTR 25.0%
! AAATTAAGCACTCTATATAG+TGG + chr4_2:21238723-21238742 None:intergenic 25.0%
AATACCACATAAGGCAAATA+GGG + chr4_2:21236166-21236185 None:intergenic 30.0%
! ACTTGTGCTTTATGAGATTT+TGG - chr4_2:21236627-21236646 Msa0594140:intron 30.0%
! TTTATGAGATTTTGGACCAA+GGG - chr4_2:21236635-21236654 Msa0594140:intron 30.0%
AATATAATATGCAGCACAAC+GGG + chr4_2:21236696-21236715 None:intergenic 30.0%
! TATTTTGCGTATGAAGTTGA+AGG - chr4_2:21236721-21236740 Msa0594140:intron 30.0%
AGTAAGAAGATGAAGAAAAG+AGG - chr4_2:21236858-21236877 Msa0594140:intron 30.0%
GTAAGAAGATGAAGAAAAGA+GGG - chr4_2:21236859-21236878 Msa0594140:intron 30.0%
CATATAAAGGCAAGAAACTT+AGG - chr4_2:21237065-21237084 Msa0594140:intron 30.0%
ATAACTCCTTGACTAAATGA+TGG - chr4_2:21237517-21237536 Msa0594140:intron 30.0%
TAACTCCTTGACTAAATGAT+GGG - chr4_2:21237518-21237537 Msa0594140:intron 30.0%
AAATTCCCATCATTTAGTCA+AGG + chr4_2:21237526-21237545 None:intergenic 30.0%
! TGACTAAATGATGGGAATTT+AGG - chr4_2:21237526-21237545 Msa0594140:intron 30.0%
!!! CAATGAGAGAAACTTTTTCA+TGG - chr4_2:21237649-21237668 Msa0594140:intron 30.0%
! GTGCTCAAAAGTTTTTCATT+TGG - chr4_2:21237803-21237822 Msa0594140:intron 30.0%
! GCTCAAAAGTTTTTCATTTG+GGG - chr4_2:21237805-21237824 Msa0594140:intron 30.0%
CCGTATCTTTAATTCTTTCA+TGG + chr4_2:21237837-21237856 None:intergenic 30.0%
ACAAGAGACTCACTTATTAA+GGG + chr4_2:21237898-21237917 None:intergenic 30.0%
TACAAGAGACTCACTTATTA+AGG + chr4_2:21237899-21237918 None:intergenic 30.0%
!! GCTTTATCGTTTTTATTCCT+AGG - chr4_2:21238555-21238574 Msa0594140:five_prime_UTR 30.0%
! ATTCGTTGCATGTATCGTTT+TGG - chr4_2:21235900-21235919 Msa0594140:exon 35.0%
AATCTATCAGCTTCTGCAAT+AGG + chr4_2:21235946-21235965 None:intergenic 35.0%
TCACTGCTTCACAACAATAT+GGG + chr4_2:21236035-21236054 None:intergenic 35.0%
TTCACTGCTTCACAACAATA+TGG + chr4_2:21236036-21236055 None:intergenic 35.0%
AAGACTTTGAATGTGGAGAA+TGG - chr4_2:21236135-21236154 Msa0594140:intron 35.0%
GAATACCACATAAGGCAAAT+AGG + chr4_2:21236167-21236186 None:intergenic 35.0%
! CTTTATGAGATTTTGGACCA+AGG - chr4_2:21236634-21236653 Msa0594140:intron 35.0%
GAGAGGACCATAACATATAA+AGG - chr4_2:21237052-21237071 Msa0594140:intron 35.0%
ATGGGAATTTAGGAAGTTGT+TGG - chr4_2:21237536-21237555 Msa0594140:intron 35.0%
!! CAAGAGTATTTACAAGGAGA+AGG + chr4_2:21238035-21238054 None:intergenic 35.0%
TCCTTGTAAATACTCTTGAG+TGG - chr4_2:21238037-21238056 Msa0594140:intron 35.0%
!! ACCACTCAAGAGTATTTACA+AGG + chr4_2:21238041-21238060 None:intergenic 35.0%
!! AACTAATACTTCTGAGACAC+TGG + chr4_2:21238437-21238456 None:intergenic 35.0%
TATTTACAGAATCAAGGGTG+TGG - chr4_2:21238661-21238680 Msa0594140:five_prime_UTR 35.0%
CCTTGAAGAAAAGGATAGCA+TGG + chr4_2:21235994-21236013 None:intergenic 40.0%
! CCATGCTATCCTTTTCTTCA+AGG - chr4_2:21235991-21236010 Msa0594140:intron 40.0%
TAGACGATCATCATAGTCGA+TGG + chr4_2:21236076-21236095 None:intergenic 40.0%
TTGTGACTGCTAGAGAGATA+TGG - chr4_2:21236101-21236120 Msa0594140:intron 40.0%
GGGTGTTTGAATACCACATA+AGG + chr4_2:21236175-21236194 None:intergenic 40.0%
ATATCGCGAGTAATCTGGTA+GGG + chr4_2:21236195-21236214 None:intergenic 40.0%
! AGTTGATATCGCGAGTAATC+TGG + chr4_2:21236200-21236219 None:intergenic 40.0%
AATAGAGTTGCACGATCCAA+CGG - chr4_2:21236917-21236936 Msa0594140:intron 40.0%
! AGGTTAAAGTGTTAACGGAG+AGG - chr4_2:21237035-21237054 Msa0594140:intron 40.0%
TTGAGCACACTCACATCAAA+TGG + chr4_2:21237791-21237810 None:intergenic 40.0%
ACTCACTTATTAAGGGACGA+AGG + chr4_2:21237891-21237910 None:intergenic 40.0%
!! TACAAGGAGAAGGTGCTAAA+AGG + chr4_2:21238025-21238044 None:intergenic 40.0%
ACTCACTTAAATTGAGACGG+AGG - chr4_2:21238289-21238308 Msa0594140:intron 40.0%
CTCACTTAAATTGAGACGGA+GGG - chr4_2:21238290-21238309 Msa0594140:intron 40.0%
GATATCGCGAGTAATCTGGT+AGG + chr4_2:21236196-21236215 None:intergenic 45.0%
GGACCAAGGGATTTCGAAAA+CGG - chr4_2:21236648-21236667 Msa0594140:intron 45.0%
GACCAAGGGATTTCGAAAAC+GGG - chr4_2:21236649-21236668 Msa0594140:intron 45.0%
!! GAGAAGGTGCTAAAAGGAGA+AGG + chr4_2:21238019-21238038 None:intergenic 45.0%
! AATCAAGGGTGTGGAAGTTG+TGG - chr4_2:21238670-21238689 Msa0594140:five_prime_UTR 45.0%
!! AAAAAAAATATATAATCTAT+AGG - chr4_2:21236340-21236359 Msa0594140:intron 5.0%
!! AAAAAAATTAATAAAGTATA+TGG - chr4_2:21236975-21236994 Msa0594140:intron 5.0%
!!! ATAAAATTTAGATTTTTTTT+TGG - chr4_2:21237424-21237443 Msa0594140:intron 5.0%
GCTGCCCTATTTGCCTTATG+TGG - chr4_2:21236159-21236178 Msa0594140:intron 50.0%
! CTCCCGTTTTCGAAATCCCT+TGG + chr4_2:21236654-21236673 None:intergenic 50.0%
CCGCACAAGGCATGCTAAAA+GGG + chr4_2:21238066-21238085 None:intergenic 50.0%
! CCCTTTTAGCATGCCTTGTG+CGG - chr4_2:21238063-21238082 Msa0594140:intron 50.0%
! CCTTTTAGCATGCCTTGTGC+GGG - chr4_2:21238064-21238083 Msa0594140:intron 50.0%
! CTTTTAGCATGCCTTGTGCG+GGG - chr4_2:21238065-21238084 Msa0594140:intron 50.0%
! TTTTAGCATGCCTTGTGCGG+GGG - chr4_2:21238066-21238085 Msa0594140:intron 50.0%
!! TTGTGGCGGATGTTGGATCA+AGG - chr4_2:21238687-21238706 Msa0594140:five_prime_UTR 50.0%
CCCGCACAAGGCATGCTAAA+AGG + chr4_2:21238067-21238086 None:intergenic 55.0%
TAGCAGATAACCCCCGCACA+AGG + chr4_2:21238079-21238098 None:intergenic 55.0%
! CAAGGGTGTGGAAGTTGTGG+CGG - chr4_2:21238673-21238692 Msa0594140:five_prime_UTR 55.0%
!! GTGGAAGTTGTGGCGGATGT+TGG - chr4_2:21238680-21238699 Msa0594140:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 21235887 21238749 21235887 ID=Msa0594140;Name=Msa0594140
chr4_2 mRNA 21235887 21238749 21235887 ID=Msa0594140-mRNA-1;Parent=Msa0594140;Name=Msa0594140-mRNA-1;_AED=0.36;_eAED=0.36;_QI=233|0.5|0.66|1|1|1|3|19|68
chr4_2 exon 21235887 21235966 21235887 ID=Msa0594140-mRNA-1:exon:5949;Parent=Msa0594140-mRNA-1
chr4_2 exon 21237778 21237816 21237778 ID=Msa0594140-mRNA-1:exon:5948;Parent=Msa0594140-mRNA-1
chr4_2 exon 21238410 21238749 21238410 ID=Msa0594140-mRNA-1:exon:5947;Parent=Msa0594140-mRNA-1
chr4_2 five_prime_UTR 21238517 21238749 21238517 ID=Msa0594140-mRNA-1:five_prime_utr;Parent=Msa0594140-mRNA-1
chr4_2 CDS 21238410 21238516 21238410 ID=Msa0594140-mRNA-1:cds;Parent=Msa0594140-mRNA-1
chr4_2 CDS 21237778 21237816 21237778 ID=Msa0594140-mRNA-1:cds;Parent=Msa0594140-mRNA-1
chr4_2 CDS 21235906 21235966 21235906 ID=Msa0594140-mRNA-1:cds;Parent=Msa0594140-mRNA-1
chr4_2 three_prime_UTR 21235887 21235905 21235887 ID=Msa0594140-mRNA-1:three_prime_utr;Parent=Msa0594140-mRNA-1
Gene Sequence

>Msa0594140

ATGGCTCTTTCTTTAAAGACTTTGAATGTGGAGAATGGTGCTGCCCTATTTGCCTTATGTGGTATTCAAACACCCTACCAGATTACTCGCGATATCAACTGTACCAAGATTATTGAACGCGATGAAGATGATTATGATGAAGATGAAATCAAGGGTGTGGAAGTTGTGGCGGATGTTGGATCAAGGAATATTGTCACCACTATATAG

Protein sequence

>Msa0594140

MALSLKTLNVENGAALFALCGIQTPYQITRDINCTKIIERDEDDYDEDEIKGVEVVADVGSRNIVTTI*