AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376640 MtrunA17_Chr3g0145781 91.176 544 21 5 1 526 3 537 0.0 999
Msa1376640 MtrunA17_Chr6g0466611 32.850 414 237 14 121 520 142 528 2.03e-52 189
Msa1376640 MtrunA17_Chr3g0107851 30.079 379 240 10 119 493 115 472 1.90e-41 156
Msa1376640 MtrunA17_Chr4g0062301 28.382 377 225 10 144 509 120 462 7.90e-41 155
Msa1376640 MtrunA17_Chr2g0330401 27.806 392 252 12 119 508 94 456 1.03e-39 150
Msa1376640 MtrunA17_Chr2g0307471 28.784 403 233 15 114 501 84 447 5.79e-39 149
Msa1376640 MtrunA17_Chr4g0063551 30.287 383 225 12 142 508 161 517 2.89e-38 149
Msa1376640 MtrunA17_Chr3g0098121 27.955 440 271 12 75 510 117 514 3.55e-36 143
Msa1376640 MtrunA17_Chr7g0270131 26.972 393 255 11 119 508 230 593 4.90e-36 142
Msa1376640 MtrunA17_Chr2g0300291 29.567 416 251 12 103 510 123 504 2.76e-35 140
Msa1376640 MtrunA17_Chr1g0157691 26.753 385 245 11 119 494 145 501 4.97e-34 135
Msa1376640 MtrunA17_Chr1g0193491 28.042 378 243 10 125 500 118 468 5.53e-34 136
Msa1376640 MtrunA17_Chr6g0449911 26.649 379 246 10 119 495 163 511 6.59e-34 136
Msa1376640 MtrunA17_Chr7g0271121 28.610 374 233 11 131 500 116 459 6.92e-34 136
Msa1376640 MtrunA17_Chr1g0200181 26.494 385 246 11 119 494 143 499 1.18e-33 134
Msa1376640 MtrunA17_Chr5g0409981 28.358 402 253 14 113 508 96 468 3.01e-33 132
Msa1376640 MtrunA17_Chr2g0293631 28.117 377 239 12 121 495 496 842 4.15e-33 134
Msa1376640 MtrunA17_Chr1g0176041 28.186 408 246 14 119 509 152 529 1.40e-32 131
Msa1376640 MtrunA17_Chr6g0483491 26.829 410 255 11 119 522 8 378 1.11e-31 127
Msa1376640 MtrunA17_Chr6g0461751 24.868 378 255 9 121 496 140 490 5.32e-31 126
Msa1376640 MtrunA17_Chr7g0236841 26.098 410 258 11 119 522 8 378 9.01e-31 124
Msa1376640 MtrunA17_Chr7g0247191 27.059 425 267 13 63 484 95 479 4.86e-30 123
Msa1376640 MtrunA17_Chr4g0040761 29.201 363 222 11 143 502 41 371 8.31e-30 124
Msa1376640 MtrunA17_Chr7g0263771 25.823 395 252 13 119 507 127 486 8.53e-30 123
Msa1376640 MtrunA17_Chr7g0247181 27.059 425 267 13 63 484 85 469 8.87e-30 122
Msa1376640 MtrunA17_Chr2g0277821 26.289 388 241 12 119 496 502 854 1.14e-29 124
Msa1376640 MtrunA17_Chr7g0247221 27.778 378 233 11 110 484 132 472 1.47e-29 122
Msa1376640 MtrunA17_Chr2g0310581 27.960 397 238 14 121 507 53 411 1.59e-29 122
Msa1376640 MtrunA17_Chr4g0034411 28.800 375 220 17 144 507 99 437 3.75e-29 122
Msa1376640 MtrunA17_Chr3g0121351 25.616 406 260 14 119 509 143 521 4.19e-29 121
Msa1376640 MtrunA17_Chr6g0475251 29.915 351 205 12 146 486 128 447 1.25e-28 120
Msa1376640 MtrunA17_Chr7g0236831 26.364 440 229 15 131 502 173 585 5.26e-28 118
Msa1376640 MtrunA17_Chr3g0114081 28.684 380 224 16 146 505 41 393 7.33e-28 117
Msa1376640 MtrunA17_Chr3g0095031 26.250 400 240 13 125 511 94 451 8.98e-28 117
Msa1376640 MtrunA17_Chr5g0443991 27.346 373 229 15 144 507 99 438 9.47e-28 117
Msa1376640 MtrunA17_Chr2g0297261 25.776 419 272 10 95 507 2 387 1.22e-27 114
Msa1376640 MtrunA17_Chr5g0442191 26.463 393 248 13 125 511 85 442 1.56e-27 116
Msa1376640 MtrunA17_Chr1g0192021 25.547 411 233 13 120 485 25 407 3.25e-27 115
Msa1376640 MtrunA17_Chr4g0058591 26.912 353 222 11 119 466 200 521 6.99e-26 111
Msa1376640 MtrunA17_Chr4g0070981 27.083 384 231 11 121 496 41 383 4.21e-25 107
Msa1376640 MtrunA17_Chr4g0029191 27.033 455 252 19 104 494 27 465 4.32e-25 109
Msa1376640 MtrunA17_Chr4g0003441 27.889 398 231 12 122 505 109 464 5.47e-25 108
Msa1376640 MtrunA17_Chr2g0299681 27.461 386 227 12 121 496 41 383 8.21e-25 106
Msa1376640 MtrunA17_Chr2g0329931 27.013 385 232 12 120 496 40 383 1.63e-24 105
Msa1376640 MtrunA17_Chr8g0347161 23.890 473 305 15 36 490 24 459 2.08e-24 107
Msa1376640 MtrunA17_Chr1g0204391 27.473 364 213 10 146 486 69 404 5.48e-24 105
Msa1376640 MtrunA17_Chr8g0342861 26.343 391 239 12 121 503 41 390 1.06e-23 103
Msa1376640 MtrunA17_Chr4g0076491 25.127 394 252 15 119 494 285 653 2.14e-23 104
Msa1376640 MtrunA17_Chr1g0149251 28.070 342 194 10 165 494 8 309 2.31e-22 98.6
Msa1376640 MtrunA17_Chr6g0449741 24.611 386 247 13 119 487 247 605 1.83e-21 98.2
Msa1376640 MtrunA17_Chr8g0347151 25.000 396 263 14 106 490 186 558 8.26e-21 96.3
Msa1376640 MtrunA17_Chr2g0309551 24.582 419 270 11 106 487 93 502 2.16e-19 91.7
Msa1376640 MtrunA17_Chr6g0465771 30.994 171 108 3 122 291 93 254 3.29e-18 86.3
Msa1376640 MtrunA17_Chr4g0074521 23.573 403 244 12 112 484 73 441 6.04e-17 83.6
Msa1376640 MtrunA17_Chr8g0364251 32.432 185 109 6 307 487 358 530 1.07e-16 83.2
Msa1376640 MtrunA17_Chr8g0364251 30.412 194 110 8 111 286 75 261 4.19e-13 72.0
Msa1376640 MtrunA17_Chr0c09g0493491 29.609 179 116 3 122 299 93 262 1.24e-16 80.9
Msa1376640 MtrunA17_Chr4g0039471 30.542 203 124 6 121 319 223 412 1.79e-16 81.6
Msa1376640 MtrunA17_Chr1g0179761 24.291 494 284 21 36 500 21 453 4.67e-15 77.8
Msa1376640 MtrunA17_Chr8g0349951 28.571 168 103 3 118 285 7 157 1.30e-14 71.6
Msa1376640 MtrunA17_Chr5g0429221 22.113 407 278 14 119 521 1 372 2.68e-14 75.1
Msa1376640 MtrunA17_Chr2g0301281 30.435 184 114 6 114 289 34 211 3.33e-13 72.4
Msa1376640 MtrunA17_Chr4g0062321 24.268 239 143 8 232 460 23 233 1.30e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376640 AT3G06980.1 66.519 451 134 3 91 526 331 779 0.0 635
Msa1376640 AT4G09730.1 31.490 416 244 12 119 520 111 499 5.26e-56 198
Msa1376640 AT1G55150.1 30.628 382 232 13 119 496 98 450 6.55e-43 160
Msa1376640 AT1G55150.2 30.628 382 232 13 119 496 123 475 2.12e-42 159
Msa1376640 AT5G63120.2 28.861 395 246 12 119 509 164 527 1.07e-38 149
Msa1376640 AT5G14610.6 28.534 382 235 13 119 495 160 508 4.30e-38 148
Msa1376640 AT5G14610.2 28.534 382 235 13 119 495 160 508 4.59e-38 148
Msa1376640 AT5G14610.5 28.534 382 235 13 119 495 168 516 7.22e-38 148
Msa1376640 AT5G14610.7 28.533 375 227 14 126 495 39 377 3.94e-36 141
Msa1376640 AT5G14610.3 28.533 375 227 14 126 495 39 377 3.94e-36 141
Msa1376640 AT3G01540.4 28.158 380 239 12 119 495 157 505 5.89e-36 142
Msa1376640 AT3G01540.2 28.158 380 239 12 119 495 157 505 5.89e-36 142
Msa1376640 AT3G01540.3 28.158 380 239 12 119 495 157 505 5.89e-36 142
Msa1376640 AT3G01540.1 28.158 380 239 12 119 495 157 505 7.00e-36 142
Msa1376640 AT2G47330.1 26.835 395 253 11 119 508 227 590 1.80e-35 141
Msa1376640 AT5G14610.1 28.689 366 223 13 135 495 244 576 3.80e-35 140
Msa1376640 AT1G31970.1 29.487 390 241 14 112 495 108 469 1.91e-34 137
Msa1376640 AT5G51280.1 26.632 383 248 11 119 494 145 501 2.60e-34 137
Msa1376640 AT5G65900.1 26.781 407 243 13 114 505 148 514 1.45e-33 135
Msa1376640 AT5G60990.1 27.540 374 241 10 119 487 8 356 4.16e-33 131
Msa1376640 AT2G42520.2 27.475 404 254 13 119 509 158 535 8.83e-33 132
Msa1376640 AT2G42520.1 27.475 404 254 13 119 509 158 535 8.83e-33 132
Msa1376640 AT2G42520.3 27.475 404 254 13 119 509 158 535 8.83e-33 132
Msa1376640 AT4G33370.1 26.371 383 249 11 119 494 96 452 1.10e-32 131
Msa1376640 AT5G26742.1 28.954 373 231 11 133 500 115 458 3.71e-32 131
Msa1376640 AT5G26742.2 28.954 373 231 11 133 500 115 458 3.71e-32 131
Msa1376640 AT3G06480.1 27.481 393 252 12 121 509 436 799 5.36e-32 131
Msa1376640 AT1G16280.1 26.847 406 253 13 111 509 51 419 9.21e-32 128
Msa1376640 AT4G00660.1 27.778 378 233 11 110 484 121 461 1.86e-31 127
Msa1376640 AT4G00660.2 27.778 378 233 11 110 484 121 461 1.86e-31 127
Msa1376640 AT3G58510.1 27.494 411 255 13 119 513 150 533 1.99e-31 129
Msa1376640 AT3G58510.3 27.494 411 255 13 119 513 150 533 1.99e-31 129
Msa1376640 AT3G58510.2 27.494 411 255 13 119 513 150 533 1.99e-31 129
Msa1376640 AT3G61240.2 27.778 378 233 10 110 484 114 454 2.18e-31 127
Msa1376640 AT3G61240.1 27.778 378 233 10 110 484 114 454 2.18e-31 127
Msa1376640 AT1G51380.1 26.720 378 244 10 119 496 21 365 2.94e-31 125
Msa1376640 AT3G18600.1 27.688 372 215 13 144 501 113 444 4.66e-31 127
Msa1376640 AT3G58570.1 27.250 400 260 12 119 509 145 522 4.91e-31 127
Msa1376640 AT5G26742.3 29.805 359 221 13 145 500 35 365 5.81e-31 127
Msa1376640 AT1G71370.1 28.643 398 219 14 131 505 27 382 6.55e-31 126
Msa1376640 AT2G45810.1 27.845 413 248 12 83 484 111 484 6.61e-31 126
Msa1376640 AT1G20920.2 26.562 384 245 12 119 496 190 542 9.08e-31 127
Msa1376640 AT1G20920.4 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT1G20920.6 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT1G20920.3 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT1G20920.7 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT1G20920.5 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT1G20920.1 26.562 384 245 12 119 496 528 880 1.49e-30 127
Msa1376640 AT5G14610.4 27.221 349 217 12 119 462 168 484 6.83e-30 123
Msa1376640 AT3G09720.2 26.582 395 249 13 119 507 136 495 2.08e-29 122
Msa1376640 AT3G09720.1 26.582 395 249 13 119 507 136 495 2.08e-29 122
Msa1376640 AT1G77030.1 28.571 357 220 10 143 496 51 375 3.17e-29 122
Msa1376640 AT3G22330.1 25.490 408 262 12 121 522 105 476 4.17e-29 122
Msa1376640 AT5G63120.1 28.739 341 209 11 119 455 164 474 6.78e-29 120
Msa1376640 AT4G16630.1 28.133 391 246 12 117 503 164 523 8.79e-29 121
Msa1376640 AT5G63120.3 28.739 341 209 11 119 455 164 474 9.43e-29 119
Msa1376640 AT5G63120.4 28.739 341 209 11 119 455 164 474 9.43e-29 119
Msa1376640 AT5G63120.5 28.739 341 209 11 119 455 164 474 9.43e-29 119
Msa1376640 AT5G54910.1 26.716 408 250 15 114 509 65 435 1.33e-28 120
Msa1376640 AT3G02065.3 27.865 384 237 13 121 496 111 462 2.91e-28 118
Msa1376640 AT3G02065.2 27.865 384 237 13 121 496 111 462 2.91e-28 118
Msa1376640 AT3G22310.2 25.000 408 264 12 121 522 117 488 7.61e-28 117
Msa1376640 AT3G22310.1 25.000 408 264 12 121 522 117 488 8.13e-28 117
Msa1376640 AT5G05450.1 26.786 392 233 14 131 500 27 386 2.31e-27 116
Msa1376640 AT3G19760.1 25.707 389 256 9 119 507 34 389 2.85e-27 114
Msa1376640 AT3G09620.2 25.393 382 217 11 122 496 399 719 3.59e-27 116
Msa1376640 AT3G09620.1 25.393 382 217 11 122 496 398 718 3.75e-27 116
Msa1376640 AT3G02065.1 28.329 353 223 12 146 496 1 325 4.72e-27 112
Msa1376640 AT2G33730.1 24.318 440 284 14 102 511 279 699 2.05e-25 110
Msa1376640 AT5G11170.1 26.355 406 241 13 105 494 29 392 4.53e-25 108
Msa1376640 AT5G11200.1 26.355 406 241 13 105 494 29 392 4.53e-25 108
Msa1376640 AT4G34910.1 24.390 410 241 11 120 485 46 430 2.06e-24 107
Msa1376640 AT3G06480.2 25.648 347 229 10 121 466 436 754 4.65e-24 107
Msa1376640 AT3G13920.1 27.083 384 233 10 120 496 39 382 6.66e-24 104
Msa1376640 AT3G13920.3 27.083 384 233 10 120 496 29 372 6.86e-24 104
Msa1376640 AT3G13920.2 27.083 384 233 10 120 496 39 382 7.48e-24 104
Msa1376640 AT3G13920.5 27.083 384 233 10 120 496 43 386 8.72e-24 104
Msa1376640 AT1G54270.1 25.995 377 246 8 120 496 39 382 1.28e-23 103
Msa1376640 AT3G16840.2 26.406 409 236 13 130 485 201 597 2.49e-23 104
Msa1376640 AT3G16840.1 26.406 409 236 13 130 485 200 596 2.53e-23 104
Msa1376640 AT2G07750.1 25.432 405 255 15 110 497 365 739 3.43e-23 104
Msa1376640 AT1G28180.1 28.451 355 213 13 166 511 19 341 4.63e-23 101
Msa1376640 AT5G62190.1 26.374 364 201 9 146 486 122 441 1.77e-22 102
Msa1376640 AT2G40700.1 25.561 446 252 21 114 494 24 454 2.06e-22 101
Msa1376640 AT5G11200.3 25.057 439 246 15 105 494 29 433 2.52e-22 100
Msa1376640 AT1G12770.2 23.587 407 267 9 116 487 106 503 3.06e-22 100
Msa1376640 AT1G12770.1 23.587 407 267 9 116 487 106 503 3.06e-22 100
Msa1376640 AT1G72730.1 26.471 374 226 11 131 496 52 384 4.45e-22 99.0
Msa1376640 AT5G08610.1 26.031 388 234 15 117 490 392 740 4.98e-22 100
Msa1376640 AT3G13920.4 27.083 384 228 11 120 496 39 377 8.88e-22 98.2
Msa1376640 AT5G11170.2 27.907 344 192 12 165 494 8 309 1.93e-21 96.3
Msa1376640 AT1G54270.2 25.995 377 241 9 120 496 39 377 1.93e-21 97.1
Msa1376640 AT1G63250.1 24.752 404 259 15 110 497 318 692 5.22e-21 97.4
Msa1376640 AT5G11200.2 23.333 450 258 14 105 494 29 451 1.44e-20 95.1
Msa1376640 AT5G63630.1 25.064 391 234 13 117 490 64 412 6.14e-20 93.6
Msa1376640 AT5G63630.2 25.316 395 228 16 117 490 258 606 7.61e-20 93.6
Msa1376640 AT5G08620.1 24.165 389 257 12 115 490 75 438 3.68e-19 91.3
Msa1376640 AT5G19210.2 24.623 398 252 14 108 484 64 434 8.56e-19 89.7
Msa1376640 AT1G51380.2 24.632 272 182 5 119 390 21 269 1.34e-18 86.7
Msa1376640 AT1G51380.4 28.293 205 127 4 119 319 21 209 8.84e-17 81.6
Msa1376640 AT1G51380.3 28.293 205 127 4 119 319 21 209 1.07e-16 81.3
Msa1376640 AT3G53110.1 23.866 419 269 13 111 513 82 466 6.40e-16 80.9
Msa1376640 AT1G59990.2 27.778 216 132 6 307 518 354 549 5.91e-15 78.2
Msa1376640 AT1G59990.1 27.778 216 132 6 307 518 354 549 6.93e-15 77.8

Find 139 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGAAGTGTCCCAGTTTA+TGG 0.122594 tig0019825:-123678 Msa1376640:CDS
CAGCATCTTTAGATGGTCTT+TGG 0.216057 tig0019825:-123076 Msa1376640:three_prime_UTR
ATATTACTTAGTAATTTCTT+AGG 0.287410 tig0019825:-123029 Msa1376640:three_prime_UTR
ATTGCTGACCAGAGTGGTTC+TGG 0.306260 tig0019825:-125600 Msa1376640:CDS
GCCCTGTCCCCTGGCACCTC+TGG 0.308184 tig0019825:+123407 None:intergenic
AGAGTGTTTAGTGGGTCTTC+TGG 0.313148 tig0019825:+126016 None:intergenic
TAGACATTGAACGACAATTA+TGG 0.323737 tig0019825:+125442 None:intergenic
TTGGAAAGGGTGCTCTCAAC+TGG 0.323947 tig0019825:-123236 Msa1376640:three_prime_UTR
TCATGAAAATAAAATTTGAT+TGG 0.327018 tig0019825:+122938 None:intergenic
ATAAACTGGGACACTTCTTT+TGG 0.327753 tig0019825:+123680 None:intergenic
TGTCCGTGCAAACCATAAAC+TGG 0.332471 tig0019825:+123666 None:intergenic
TTGCAGAACACAATAGTTCT+TGG 0.346234 tig0019825:+123945 None:intergenic
TCATTTGCTCCTGTTATTGC+CGG 0.349281 tig0019825:-125636 Msa1376640:CDS
AGCATCTTTAGATGGTCTTT+GGG 0.352962 tig0019825:-123075 Msa1376640:three_prime_UTR
GATGAGGTTGATATCCTCTT+TGG 0.353589 tig0019825:-125094 Msa1376640:CDS
GAGTGTTTAGTGGGTCTTCT+GGG 0.364053 tig0019825:+126017 None:intergenic
GGTAGCACCGACAATATTCT+AGG 0.364067 tig0019825:-123157 Msa1376640:three_prime_UTR
GGCGAGTTAAAGAGAGGTTA+TGG 0.368794 tig0019825:+125679 None:intergenic
CAAGACTTTCCGGCAATAAC+AGG 0.372706 tig0019825:+125627 None:intergenic
AAAAGAGAAGAGAATCAAAA+TGG 0.386747 tig0019825:-126102 Msa1376640:exon
AGCAATTATACAAGACTTTC+CGG 0.389114 tig0019825:+125617 None:intergenic
GGCAAAGGAAGGAAATCTTT+TGG 0.389304 tig0019825:-123178 Msa1376640:three_prime_UTR
CATGAAAATAAAATTTGATT+GGG 0.393804 tig0019825:+122939 None:intergenic
AGCCAGAGGTGCCAGGGGAC+AGG 0.398550 tig0019825:-123409 Msa1376640:CDS
GTCGATAAAACCACGAGTTT+CGG 0.403323 tig0019825:-125942 Msa1376640:CDS
CACAGCTGAGTTAGCTTCTC+AGG 0.409704 tig0019825:-125469 Msa1376640:CDS
AATTACTAAGTAATATGATT+AGG 0.415417 tig0019825:+123036 None:intergenic
TGTCGTTCAATGTCTAAATC+TGG 0.417609 tig0019825:-125435 Msa1376640:CDS
GAGTTGGATGGGGAGACAAT+AGG 0.426703 tig0019825:-125788 Msa1376640:CDS
AAGGCATTTATCTTTGTTGT+TGG 0.426934 tig0019825:-123384 Msa1376640:CDS
TGCAGAACACAATAGTTCTT+GGG 0.438418 tig0019825:+123946 None:intergenic
CCCATGACCATTTCACAATC+AGG 0.439831 tig0019825:+124950 None:intergenic
GAGCTGAAGAAGTAGGAAGT+TGG 0.443767 tig0019825:-123273 Msa1376640:three_prime_UTR
TGATTGCCAGCATCTTTAGA+TGG 0.444955 tig0019825:-123083 Msa1376640:three_prime_UTR
TCCACTGCATGATGTACCTT+CGG 0.449294 tig0019825:-123310 Msa1376640:CDS
GAGAGATTCAATCATGAAAT+CGG 0.449692 tig0019825:+125701 None:intergenic
AGTTGGATGGGGAGACAATA+GGG 0.452318 tig0019825:-125787 Msa1376640:CDS
GAAGAACAGCCAGAGGTGCC+AGG 0.461221 tig0019825:-123416 Msa1376640:CDS
CTAAAATCATTTAAAGACAT+TGG 0.461700 tig0019825:-125729 Msa1376640:CDS
AGTGAAACCTGACCTCTTCA+AGG 0.469671 tig0019825:+124900 None:intergenic
AACTCGCCCTTCCAACATAC+AGG 0.470926 tig0019825:-125664 Msa1376640:CDS
AACTCATTCGAGAGTTGGAT+GGG 0.471462 tig0019825:-125799 Msa1376640:CDS
AAATGCCTTGCCCTGTCCCC+TGG 0.473330 tig0019825:+123398 None:intergenic
TGTCTGAGACGTTGAATGAT+TGG 0.476607 tig0019825:+125555 None:intergenic
AGTTTATGGTTTGCACGGAC+AGG 0.484625 tig0019825:-123664 Msa1376640:intron
AACTTGTAACAGAGCTTCGC+GGG 0.488229 tig0019825:-123517 Msa1376640:intron
AGGTGCCAGGGGACAGGGCA+AGG 0.488567 tig0019825:-123403 Msa1376640:CDS
GAATTCCCTTTCTATCAAAA+CGG 0.499892 tig0019825:+123783 None:intergenic
TCTTTAGATGGTCTTTGGGC+AGG 0.501801 tig0019825:-123071 Msa1376640:three_prime_UTR
GCCAGAGGTGCCAGGGGACA+GGG 0.502782 tig0019825:-123408 Msa1376640:CDS
ATTATGTTGCAGTGCTGTCT+TGG 0.503149 tig0019825:-125116 Msa1376640:intron
TTGCTGTTTAGCATCGGAGT+TGG 0.503766 tig0019825:-126047 Msa1376640:CDS
TCCTGATTGTGAAATGGTCA+TGG 0.506213 tig0019825:-124951 Msa1376640:CDS
GTAGATTGTAGTGGGGAAGA+TGG 0.506554 tig0019825:-124044 Msa1376640:CDS
AGCTGCTCTTCTGCAGCTTG+CGG 0.507830 tig0019825:-123982 Msa1376640:CDS
TTTAATTGATTGCTTGGAAA+GGG 0.510457 tig0019825:-123249 Msa1376640:three_prime_UTR
TCATCGACCTAGAATATTGT+CGG 0.512080 tig0019825:+123150 None:intergenic
TGGCGATGAGGACTTCGAAA+AGG 0.514933 tig0019825:-125074 Msa1376640:CDS
AGATAATAGTAGATTGTAGT+GGG 0.515530 tig0019825:-124052 Msa1376640:CDS
ATGCTTGATTAGTTCTTCAC+CGG 0.515910 tig0019825:-125044 Msa1376640:CDS
CAACTCATTCGAGAGTTGGA+TGG 0.516133 tig0019825:-125800 Msa1376640:CDS
CAAAGACCATCTAAAGATGC+TGG 0.522860 tig0019825:+123077 None:intergenic
GGTTGATGAGGAAGAGAAAA+CGG 0.523534 tig0019825:+125310 None:intergenic
CTTAGGAATTGTATTACCAC+CGG 0.524593 tig0019825:-123012 Msa1376640:three_prime_UTR
ACTGCAACTCATTCGAGAGT+TGG 0.528094 tig0019825:-125804 Msa1376640:CDS
GAAGAAGACAGAGTGTTTAG+TGG 0.529879 tig0019825:+126007 None:intergenic
CAATAGGGTTAGCAGTCAAT+CGG 0.532781 tig0019825:-125772 Msa1376640:CDS
GGCTCCTCATAGCAAAAGCA+AGG 0.533958 tig0019825:-123113 Msa1376640:three_prime_UTR
AGTGAATATGTACGGCGTGT+TGG 0.534281 tig0019825:-123438 Msa1376640:CDS
CCTGATTGTGAAATGGTCAT+GGG 0.535391 tig0019825:-124950 Msa1376640:CDS
GTCCCAGTTTATGGTTTGCA+CGG 0.536407 tig0019825:-123669 Msa1376640:CDS
GTGACAAGCATAGATTTGAA+TGG 0.537448 tig0019825:+125405 None:intergenic
AAATCTATGCTTGTCACTGG+TGG 0.541886 tig0019825:-125399 Msa1376640:CDS
AAGAAGACAGAGTGTTTAGT+GGG 0.547041 tig0019825:+126008 None:intergenic
ATCACGGCTTACTAGTATGA+AGG 0.552151 tig0019825:-123720 Msa1376640:CDS
TTGCAGTGCTGTCTTGGATG+AGG 0.552233 tig0019825:-125110 Msa1376640:intron
CGAAGTCCTCATCGCCAAAG+AGG 0.554253 tig0019825:+125080 None:intergenic
CAGATAATAGTAGATTGTAG+TGG 0.557322 tig0019825:-124053 Msa1376640:CDS
TTCAAATCTATGCTTGTCAC+TGG 0.558308 tig0019825:-125402 Msa1376640:CDS
GTGGTTGCGCAAGGTGTTTG+TGG 0.559691 tig0019825:-126077 Msa1376640:CDS
GTATTGAGAGCTGAAGAAGT+AGG 0.560738 tig0019825:-123280 Msa1376640:exon
TGGAAGGGCGAGTTAAAGAG+AGG 0.560834 tig0019825:+125673 None:intergenic
AAATGACATGGCCTGTATGT+TGG 0.565806 tig0019825:+125653 None:intergenic
AGTAATGGAAAGAAATCGGA+AGG 0.566217 tig0019825:-123337 Msa1376640:CDS
TCGATGAAACGTGCATTGCT+AGG 0.568808 tig0019825:-123134 Msa1376640:three_prime_UTR
AAGAACAGCCAGAGGTGCCA+GGG 0.570215 tig0019825:-123415 Msa1376640:CDS
GAACTTATTCGATGCATACT+AGG 0.571644 tig0019825:+124926 None:intergenic
AATAAATATTGTGTGTCCAC+CGG 0.571880 tig0019825:+125025 None:intergenic
CAACTTGTAACAGAGCTTCG+CGG 0.572338 tig0019825:-123518 Msa1376640:intron
TGGAAAGGGTGCTCTCAACT+GGG 0.579482 tig0019825:-123235 Msa1376640:three_prime_UTR
AAGTCTAACATGCATGGCAA+AGG 0.579671 tig0019825:-123193 Msa1376640:three_prime_UTR
CTTGATTAGTTCTTCACCGG+TGG 0.581413 tig0019825:-125041 Msa1376640:CDS
AAAGCGTTGCTGTTTAGCAT+CGG 0.582268 tig0019825:-126053 Msa1376640:CDS
TGTGTCATGGCAGCATGGAA+AGG 0.582945 tig0019825:+123746 None:intergenic
CTCAGACAACAAGAACTACA+AGG 0.588339 tig0019825:-125540 Msa1376640:CDS
TGATATCCTCTTTGGCGATG+AGG 0.588829 tig0019825:-125086 Msa1376640:CDS
CTAACATGCATGGCAAAGGA+AGG 0.589079 tig0019825:-123189 Msa1376640:three_prime_UTR
TGCACTTAACTAATCTAACA+TGG 0.590356 tig0019825:-125278 Msa1376640:intron
ATGTCTTTCCAGAACCACTC+TGG 0.590455 tig0019825:+125592 None:intergenic
AATAAGTTCTCGCCTTGAAG+AGG 0.590513 tig0019825:-124912 Msa1376640:intron
TGCAGGAGTGTCATTTCCGG+TGG 0.590891 tig0019825:+122996 None:intergenic
CTGAGAAGCTAACTCAGCTG+TGG 0.591686 tig0019825:+125470 None:intergenic
TTTCTCTTCCTCATCAACCA+AGG 0.592789 tig0019825:-125306 Msa1376640:CDS
AAGCCGTGATTCTTGTGTCA+TGG 0.593997 tig0019825:+123733 None:intergenic
TATTGTAAGTCTAACATGCA+TGG 0.594332 tig0019825:-123199 Msa1376640:three_prime_UTR
ACACGCCGTACATATTCACT+TGG 0.594509 tig0019825:+123441 None:intergenic
GGGAATTGACTTCACTAGAG+TGG 0.597817 tig0019825:-123496 Msa1376640:CDS
TCAACTCGACACTTTAAACC+AGG 0.598198 tig0019825:-125361 Msa1376640:CDS
GTCCGTGCAAACCATAAACT+GGG 0.602781 tig0019825:+123667 None:intergenic
GGTGCGAGTATAACAAGAGT+GGG 0.603768 tig0019825:+125492 None:intergenic
ACATATTCACTTGGATCACG+TGG 0.605024 tig0019825:+123450 None:intergenic
GTGATCCAAGTGAATATGTA+CGG 0.607674 tig0019825:-123446 Msa1376640:CDS
TTCAATGTCTAAATCTGGCA+AGG 0.610738 tig0019825:-125430 Msa1376640:CDS
ATGAGGAAGAGAAAACGGCC+AGG 0.612771 tig0019825:+125315 None:intergenic
TGAGAAGCTAACTCAGCTGT+GGG 0.613832 tig0019825:+125471 None:intergenic
AAAATGGCGGTGGTTGCGCA+AGG 0.615353 tig0019825:-126086 Msa1376640:CDS
GAAGAGAATCAAAATGGCGG+TGG 0.615716 tig0019825:-126096 Msa1376640:exon
GACATGGCCTGTATGTTGGA+AGG 0.625996 tig0019825:+125657 None:intergenic
GATGTCTTCATAGCTACACC+TGG 0.633711 tig0019825:-125333 Msa1376640:CDS
CTGCCATGACACAAGAATCA+CGG 0.635761 tig0019825:-123736 Msa1376640:CDS
GATAATAGTAGATTGTAGTG+GGG 0.637024 tig0019825:-124051 Msa1376640:CDS
AGAACAGCCAGAGGTGCCAG+GGG 0.638006 tig0019825:-123414 Msa1376640:CDS
GCCGAAGGTACATCATGCAG+TGG 0.640301 tig0019825:+123309 None:intergenic
TGTATAATTGCTGACCAGAG+TGG 0.648838 tig0019825:-125606 Msa1376640:CDS
GGGTGCGAGTATAACAAGAG+TGG 0.651197 tig0019825:+125491 None:intergenic
TCTCAATACTCATAAGCCGA+AGG 0.655130 tig0019825:+123294 None:intergenic
ACATGGCCTGTATGTTGGAA+GGG 0.662222 tig0019825:+125658 None:intergenic
TAGGGTTAGCAGTCAATCGG+AGG 0.663524 tig0019825:-125769 Msa1376640:CDS
ATTCTTGTGTCATGGCAGCA+TGG 0.663708 tig0019825:+123741 None:intergenic
ACTCATTCGAGAGTTGGATG+GGG 0.667637 tig0019825:-125798 Msa1376640:CDS
CGTGTTGGAAGAACAGCCAG+AGG 0.669248 tig0019825:-123423 Msa1376640:CDS
GCTCCTCATAGCAAAAGCAA+GGG 0.695618 tig0019825:-123112 Msa1376640:three_prime_UTR
CAACTCGACACTTTAAACCA+GGG 0.695695 tig0019825:-125360 Msa1376640:CDS
GTAATGGAAAGAAATCGGAA+GGG 0.700702 tig0019825:-123336 Msa1376640:CDS
AATAACAGGAGCAAATGACA+TGG 0.700797 tig0019825:+125641 None:intergenic
ACTTGTAACAGAGCTTCGCG+GGG 0.711965 tig0019825:-123516 Msa1376640:intron
CATATTCACTTGGATCACGT+GGG 0.715544 tig0019825:+123451 None:intergenic
AGAGAAGAGAATCAAAATGG+CGG 0.724812 tig0019825:-126099 Msa1376640:exon
CTATGAAGACATCAACACCC+TGG 0.726139 tig0019825:+125343 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCATAATATGAAATAAA+AGG - tig0019825:124569-124588 Msa1376640:intron 10.0%
!! ATTCATAATATGAAATAAAA+GGG - tig0019825:124570-124589 Msa1376640:intron 10.0%
!!! AATATTAATTTGAAATTAAG+TGG + tig0019825:124879-124898 None:intergenic 10.0%
!! CAAAAAATTACAAATATAAT+AGG - tig0019825:126060-126079 Msa1376640:CDS 10.0%
!! GTTAAGATAAATTAAATCAT+TGG + tig0019825:123889-123908 None:intergenic 15.0%
!!! ATTTCTATTTTATTCATCTT+AGG - tig0019825:124172-124191 Msa1376640:intron 15.0%
!!! GTTATTGTTTTACTATTTAT+AGG - tig0019825:124641-124660 Msa1376640:intron 15.0%
!! TAATATATTCTTAAATTGCT+GGG - tig0019825:124806-124825 Msa1376640:intron 15.0%
!!! CTTTTTGTTACTTTTTATTT+TGG - tig0019825:125168-125187 Msa1376640:intron 15.0%
!! AATTACTAAGTAATATGATT+AGG + tig0019825:126002-126021 None:intergenic 15.0%
!! ATATTACTTAGTAATTTCTT+AGG - tig0019825:126006-126025 Msa1376640:CDS 15.0%
!!! TTATATTTGTAATTTTTTGC+AGG + tig0019825:126059-126078 None:intergenic 15.0%
!! CATGAAAATAAAATTTGATT+GGG + tig0019825:126099-126118 None:intergenic 15.0%
!! TCATGAAAATAAAATTTGAT+TGG + tig0019825:126100-126119 None:intergenic 15.0%
!!! TTTCAATTTTCAACAAGATA+AGG + tig0019825:122902-122921 None:intergenic 20.0%
!! CTAAAATCATTTAAAGACAT+TGG - tig0019825:123306-123325 Msa1376640:CDS 20.0%
!! AAAATGATATGTGATAGAAA+AGG + tig0019825:123799-123818 None:intergenic 20.0%
!! AGAAATCAATTTATAACACA+TGG + tig0019825:123853-123872 None:intergenic 20.0%
!! ATTCAATTTAAATCTCATGT+GGG + tig0019825:124286-124305 None:intergenic 20.0%
!! AATTCAATTTAAATCTCATG+TGG + tig0019825:124287-124306 None:intergenic 20.0%
!! ATACACAATGATATGTATAA+AGG + tig0019825:124325-124344 None:intergenic 20.0%
!! TTTATACATATCATTGTGTA+TGG - tig0019825:124324-124343 Msa1376640:intron 20.0%
!! TCCAGTATTAAATTTATTAG+TGG + tig0019825:124488-124507 None:intergenic 20.0%
!!! GTATTTCTTTTTGAATAGTA+TGG + tig0019825:124513-124532 None:intergenic 20.0%
!!! GAAATTTTGAGAAATGTTTA+GGG + tig0019825:124543-124562 None:intergenic 20.0%
!!! TGAAATTTTGAGAAATGTTT+AGG + tig0019825:124544-124563 None:intergenic 20.0%
!! TTGTACTAGTTGTATATAAT+TGG + tig0019825:124621-124640 None:intergenic 20.0%
!! GCAATTTAAGAATATATTAC+AGG + tig0019825:124805-124824 None:intergenic 20.0%
!! GTAATATATTCTTAAATTGC+TGG - tig0019825:124805-124824 Msa1376640:intron 20.0%
!! AATATATTCTTAAATTGCTG+GGG - tig0019825:124807-124826 Msa1376640:intron 20.0%
!!! AAAAAGTAATGGAAAGAAAT+CGG - tig0019825:125694-125713 Msa1376640:CDS 20.0%
! AAAAGAGAAGAGAATCAAAA+TGG - tig0019825:122933-122952 Msa1376640:three_prime_UTR 25.0%
!! TGATTTTAGACTGAAAAAGT+TGG + tig0019825:123295-123314 None:intergenic 25.0%
! TTAACTAATCTAACATGGTA+TGG - tig0019825:123762-123781 Msa1376640:CDS 25.0%
! GATAAATTAAATCATTGGTG+CGG + tig0019825:123884-123903 None:intergenic 25.0%
!!! AGTTTGCTGTAAACATTTTT+TGG + tig0019825:124052-124071 None:intergenic 25.0%
! AAAAAATGTTTACAGCAAAC+TGG - tig0019825:124051-124070 Msa1376640:CDS 25.0%
!!! GTTGGTAACAATTATGAAAA+TGG - tig0019825:124360-124379 Msa1376640:intron 25.0%
! GCCACTAATAAATTTAATAC+TGG - tig0019825:124484-124503 Msa1376640:intron 25.0%
! AGATAATAGTAGATTGTAGT+GGG - tig0019825:124983-125002 Msa1376640:CDS 25.0%
!!! ATTAAACCGTTTTGATAGAA+AGG - tig0019825:125246-125265 Msa1376640:intron 25.0%
!!! TTAAACCGTTTTGATAGAAA+GGG - tig0019825:125247-125266 Msa1376640:intron 25.0%
! GTGTTACAAATAATTCATTG+TGG - tig0019825:125494-125513 Msa1376640:CDS 25.0%
!!! TTTTAATTGATTGCTTGGAA+AGG - tig0019825:125785-125804 Msa1376640:CDS 25.0%
! TTTAATTGATTGCTTGGAAA+GGG - tig0019825:125786-125805 Msa1376640:CDS 25.0%
GAGAGATTCAATCATGAAAT+CGG + tig0019825:123337-123356 None:intergenic 30.0%
AGCAATTATACAAGACTTTC+CGG + tig0019825:123421-123440 None:intergenic 30.0%
TAGACATTGAACGACAATTA+TGG + tig0019825:123596-123615 None:intergenic 30.0%
TGCACTTAACTAATCTAACA+TGG - tig0019825:123757-123776 Msa1376640:CDS 30.0%
AATAAATATTGTGTGTCCAC+CGG + tig0019825:124013-124032 None:intergenic 30.0%
! AGTTTTTCCTGATTGTGAAA+TGG - tig0019825:124078-124097 Msa1376640:intron 30.0%
CTGTGATATGAGAAAACATT+TGG - tig0019825:124244-124263 Msa1376640:intron 30.0%
AGGGATAGTAAAATCGAAAA+TGG - tig0019825:124589-124608 Msa1376640:intron 30.0%
TGAAATTAAGTGGTCATTAC+TGG + tig0019825:124869-124888 None:intergenic 30.0%
CAGATAATAGTAGATTGTAG+TGG - tig0019825:124982-125001 Msa1376640:CDS 30.0%
GATAATAGTAGATTGTAGTG+GGG - tig0019825:124984-125003 Msa1376640:CDS 30.0%
GAATTCCCTTTCTATCAAAA+CGG + tig0019825:125255-125274 None:intergenic 30.0%
! AAGGCATTTATCTTTGTTGT+TGG - tig0019825:125651-125670 Msa1376640:CDS 30.0%
!!! TTCCATTACTTTTTGTGCAA+GGG + tig0019825:125688-125707 None:intergenic 30.0%
!!! TTTCCATTACTTTTTGTGCA+AGG + tig0019825:125689-125708 None:intergenic 30.0%
!!! GTTGGTTTTAATTGATTGCT+TGG - tig0019825:125780-125799 Msa1376640:CDS 30.0%
TATTGTAAGTCTAACATGCA+TGG - tig0019825:125836-125855 Msa1376640:CDS 30.0%
AGAGAAGAGAATCAAAATGG+CGG - tig0019825:122936-122955 Msa1376640:three_prime_UTR 35.0%
! AAGAAGACAGAGTGTTTAGT+GGG + tig0019825:123030-123049 None:intergenic 35.0%
!! GGCGGATTTTGAATTTGAAT+TGG + tig0019825:123073-123092 None:intergenic 35.0%
! TTGTAGTTGTTTGCGTTTTG+TGG + tig0019825:123172-123191 None:intergenic 35.0%
AATAACAGGAGCAAATGACA+TGG + tig0019825:123397-123416 None:intergenic 35.0%
TGTCGTTCAATGTCTAAATC+TGG - tig0019825:123600-123619 Msa1376640:intron 35.0%
TTCAATGTCTAAATCTGGCA+AGG - tig0019825:123605-123624 Msa1376640:intron 35.0%
GTGACAAGCATAGATTTGAA+TGG + tig0019825:123633-123652 None:intergenic 35.0%
TTCAAATCTATGCTTGTCAC+TGG - tig0019825:123633-123652 Msa1376640:intron 35.0%
!!! TGTCGAGTTGAGTTTTTTGT+CGG + tig0019825:123665-123684 None:intergenic 35.0%
TAGTTAAGTGCAAAAAGCCT+TGG + tig0019825:123749-123768 None:intergenic 35.0%
GGTAAATGACAAGTGTATTC+AGG + tig0019825:123832-123851 None:intergenic 35.0%
! ATGCTTGATTAGTTCTTCAC+CGG - tig0019825:123991-124010 Msa1376640:CDS 35.0%
GAACTTATTCGATGCATACT+AGG + tig0019825:124112-124131 None:intergenic 35.0%
AACGTATTCCCTAAATTTGC+TGG - tig0019825:124209-124228 Msa1376640:intron 35.0%
TACTATTTATAGGTCCGCTT+TGG - tig0019825:124651-124670 Msa1376640:intron 35.0%
!! CTTAAATTGCTGGGGTATTT+TGG - tig0019825:124815-124834 Msa1376640:intron 35.0%
TGCAGAACACAATAGTTCTT+GGG + tig0019825:125092-125111 None:intergenic 35.0%
TTGCAGAACACAATAGTTCT+TGG + tig0019825:125093-125112 None:intergenic 35.0%
! ACAAAGTATTCTGAACTCTC+AGG - tig0019825:125112-125131 Msa1376640:intron 35.0%
! ATAAACTGGGACACTTCTTT+TGG + tig0019825:125358-125377 None:intergenic 35.0%
!! AAAAGAAGTGTCCCAGTTTA+TGG - tig0019825:125357-125376 Msa1376640:CDS 35.0%
!! TATTCATCTGTTTTTCTCGC+TGG - tig0019825:125394-125413 Msa1376640:CDS 35.0%
GTGATCCAAGTGAATATGTA+CGG - tig0019825:125589-125608 Msa1376640:CDS 35.0%
ATCCCTTGCACAAAAAGTAA+TGG - tig0019825:125683-125702 Msa1376640:CDS 35.0%
! AGTAATGGAAAGAAATCGGA+AGG - tig0019825:125698-125717 Msa1376640:CDS 35.0%
! GTAATGGAAAGAAATCGGAA+GGG - tig0019825:125699-125718 Msa1376640:CDS 35.0%
TCATCGACCTAGAATATTGT+CGG + tig0019825:125888-125907 None:intergenic 35.0%
AGCATCTTTAGATGGTCTTT+GGG - tig0019825:125960-125979 Msa1376640:CDS 35.0%
CTTAGGAATTGTATTACCAC+CGG - tig0019825:126023-126042 Msa1376640:CDS 35.0%
!! AAAGCGTTGCTGTTTAGCAT+CGG - tig0019825:122982-123001 Msa1376640:three_prime_UTR 40.0%
! GAAGAAGACAGAGTGTTTAG+TGG + tig0019825:123031-123050 None:intergenic 40.0%
!!! GAAACTCGTGGTTTTATCGA+CGG + tig0019825:123094-123113 None:intergenic 40.0%
GTCGATAAAACCACGAGTTT+CGG - tig0019825:123093-123112 Msa1376640:three_prime_UTR 40.0%
!! TTTTAGTCTTCCGAAACTCG+TGG + tig0019825:123106-123125 None:intergenic 40.0%
!!! TGGTATTTTGCTTGGTTCTG+TGG + tig0019825:123152-123171 None:intergenic 40.0%
! GCGTTTTGTGGTATTTTGCT+TGG + tig0019825:123160-123179 None:intergenic 40.0%
AACTCATTCGAGAGTTGGAT+GGG - tig0019825:123236-123255 Msa1376640:three_prime_UTR 40.0%
! CAATAGGGTTAGCAGTCAAT+CGG - tig0019825:123263-123282 Msa1376640:three_prime_UTR 40.0%
AAATGACATGGCCTGTATGT+TGG + tig0019825:123385-123404 None:intergenic 40.0%
TCATTTGCTCCTGTTATTGC+CGG - tig0019825:123399-123418 Msa1376640:CDS 40.0%
TGTATAATTGCTGACCAGAG+TGG - tig0019825:123429-123448 Msa1376640:CDS 40.0%
! TGTCTGAGACGTTGAATGAT+TGG + tig0019825:123483-123502 None:intergenic 40.0%
CTCAGACAACAAGAACTACA+AGG - tig0019825:123495-123514 Msa1376640:CDS 40.0%
AAATCTATGCTTGTCACTGG+TGG - tig0019825:123636-123655 Msa1376640:intron 40.0%
TCAACTCGACACTTTAAACC+AGG - tig0019825:123674-123693 Msa1376640:CDS 40.0%
CAACTCGACACTTTAAACCA+GGG - tig0019825:123675-123694 Msa1376640:CDS 40.0%
!! GGTTGATGAGGAAGAGAAAA+CGG + tig0019825:123728-123747 None:intergenic 40.0%
TTTCTCTTCCTCATCAACCA+AGG - tig0019825:123729-123748 Msa1376640:CDS 40.0%
ATTATGTTGCAGTGCTGTCT+TGG - tig0019825:123919-123938 Msa1376640:intron 40.0%
!! GATGAGGTTGATATCCTCTT+TGG - tig0019825:123941-123960 Msa1376640:CDS 40.0%
TCCTGATTGTGAAATGGTCA+TGG - tig0019825:124084-124103 Msa1376640:intron 40.0%
CCTGATTGTGAAATGGTCAT+GGG - tig0019825:124085-124104 Msa1376640:intron 40.0%
AATAAGTTCTCGCCTTGAAG+AGG - tig0019825:124123-124142 Msa1376640:intron 40.0%
!! TATTCCCTAAATTTGCTGGC+TGG - tig0019825:124213-124232 Msa1376640:intron 40.0%
!! ATTCCCTAAATTTGCTGGCT+GGG - tig0019825:124214-124233 Msa1376640:intron 40.0%
AATCTCATGTGGGAGACAAA+AGG + tig0019825:124276-124295 None:intergenic 40.0%
CATATCATTGTGTATGGCAG+TGG - tig0019825:124330-124349 Msa1376640:intron 40.0%
GGTGCAGTCAACTCAATAAT+TGG - tig0019825:124425-124444 Msa1376640:intron 40.0%
AGTCAACTCAATAATTGGCG+CGG - tig0019825:124430-124449 Msa1376640:intron 40.0%
ATAATCAGCTGCTGTGTAAG+TGG - tig0019825:124933-124952 Msa1376640:CDS 40.0%
TAATCAGCTGCTGTGTAAGT+GGG - tig0019825:124934-124953 Msa1376640:CDS 40.0%
ATCACGGCTTACTAGTATGA+AGG - tig0019825:125315-125334 Msa1376640:CDS 40.0%
CATATTCACTTGGATCACGT+GGG + tig0019825:125587-125606 None:intergenic 40.0%
ACATATTCACTTGGATCACG+TGG + tig0019825:125588-125607 None:intergenic 40.0%
TCTCAATACTCATAAGCCGA+AGG + tig0019825:125744-125763 None:intergenic 40.0%
GTATTGAGAGCTGAAGAAGT+AGG - tig0019825:125755-125774 Msa1376640:CDS 40.0%
AAGTCTAACATGCATGGCAA+AGG - tig0019825:125842-125861 Msa1376640:CDS 40.0%
! GGCAAAGGAAGGAAATCTTT+TGG - tig0019825:125857-125876 Msa1376640:CDS 40.0%
TGATTGCCAGCATCTTTAGA+TGG - tig0019825:125952-125971 Msa1376640:CDS 40.0%
CAAAGACCATCTAAAGATGC+TGG + tig0019825:125961-125980 None:intergenic 40.0%
CAGCATCTTTAGATGGTCTT+TGG - tig0019825:125959-125978 Msa1376640:CDS 40.0%
!!! TTTTGCAGGAGTGTCATTTC+CGG + tig0019825:126045-126064 None:intergenic 40.0%
GAAGAGAATCAAAATGGCGG+TGG - tig0019825:122939-122958 Msa1376640:three_prime_UTR 45.0%
!! TTGCTGTTTAGCATCGGAGT+TGG - tig0019825:122988-123007 Msa1376640:three_prime_UTR 45.0%
! GAGTGTTTAGTGGGTCTTCT+GGG + tig0019825:123021-123040 None:intergenic 45.0%
! AGAGTGTTTAGTGGGTCTTC+TGG + tig0019825:123022-123041 None:intergenic 45.0%
ACTGCAACTCATTCGAGAGT+TGG - tig0019825:123231-123250 Msa1376640:three_prime_UTR 45.0%
CAACTCATTCGAGAGTTGGA+TGG - tig0019825:123235-123254 Msa1376640:three_prime_UTR 45.0%
ACTCATTCGAGAGTTGGATG+GGG - tig0019825:123237-123256 Msa1376640:three_prime_UTR 45.0%
AGTTGGATGGGGAGACAATA+GGG - tig0019825:123248-123267 Msa1376640:three_prime_UTR 45.0%
GGCGAGTTAAAGAGAGGTTA+TGG + tig0019825:123359-123378 None:intergenic 45.0%
ACATGGCCTGTATGTTGGAA+GGG + tig0019825:123380-123399 None:intergenic 45.0%
CAAGACTTTCCGGCAATAAC+AGG + tig0019825:123411-123430 None:intergenic 45.0%
ATGTCTTTCCAGAACCACTC+TGG + tig0019825:123446-123465 None:intergenic 45.0%
GGTGCGAGTATAACAAGAGT+GGG + tig0019825:123546-123565 None:intergenic 45.0%
TGAGAAGCTAACTCAGCTGT+GGG + tig0019825:123567-123586 None:intergenic 45.0%
CTATGAAGACATCAACACCC+TGG + tig0019825:123695-123714 None:intergenic 45.0%
GATGTCTTCATAGCTACACC+TGG - tig0019825:123702-123721 Msa1376640:CDS 45.0%
! GCAAAAAGCCTTGGTTGATG+AGG + tig0019825:123740-123759 None:intergenic 45.0%
TGATATCCTCTTTGGCGATG+AGG - tig0019825:123949-123968 Msa1376640:CDS 45.0%
! CTTGATTAGTTCTTCACCGG+TGG - tig0019825:123994-124013 Msa1376640:CDS 45.0%
CCCATGACCATTTCACAATC+AGG + tig0019825:124088-124107 None:intergenic 45.0%
AGTGAAACCTGACCTCTTCA+AGG + tig0019825:124138-124157 None:intergenic 45.0%
AAGCCCAGCCAGCAAATTTA+GGG + tig0019825:124220-124239 None:intergenic 45.0%
ACCTGTCTCTGTTGTCAATG+CGG - tig0019825:124391-124410 Msa1376640:intron 45.0%
CTGTTGTCAATGCGGAAATG+CGG - tig0019825:124399-124418 Msa1376640:intron 45.0%
AAAGTTGAAAGCGGCCAAAG+CGG + tig0019825:124668-124687 None:intergenic 45.0%
CGCGTGTCAAAAGTTGAAAG+CGG + tig0019825:124677-124696 None:intergenic 45.0%
! GTAGATTGTAGTGGGGAAGA+TGG - tig0019825:124991-125010 Msa1376640:CDS 45.0%
ATTCTTGTGTCATGGCAGCA+TGG + tig0019825:125297-125316 None:intergenic 45.0%
CTGCCATGACACAAGAATCA+CGG - tig0019825:125299-125318 Msa1376640:CDS 45.0%
AAGCCGTGATTCTTGTGTCA+TGG + tig0019825:125305-125324 None:intergenic 45.0%
! AACTGGGACACTTCTTTTGG+TGG + tig0019825:125355-125374 None:intergenic 45.0%
! GTCCCAGTTTATGGTTTGCA+CGG - tig0019825:125366-125385 Msa1376640:CDS 45.0%
GTCCGTGCAAACCATAAACT+GGG + tig0019825:125371-125390 None:intergenic 45.0%
TGTCCGTGCAAACCATAAAC+TGG + tig0019825:125372-125391 None:intergenic 45.0%
! AGTTTATGGTTTGCACGGAC+AGG - tig0019825:125371-125390 Msa1376640:CDS 45.0%
CAACTTGTAACAGAGCTTCG+CGG - tig0019825:125517-125536 Msa1376640:CDS 45.0%
AACTTGTAACAGAGCTTCGC+GGG - tig0019825:125518-125537 Msa1376640:CDS 45.0%
! GGGAATTGACTTCACTAGAG+TGG - tig0019825:125539-125558 Msa1376640:CDS 45.0%
ACACGCCGTACATATTCACT+TGG + tig0019825:125597-125616 None:intergenic 45.0%
AGTGAATATGTACGGCGTGT+TGG - tig0019825:125597-125616 Msa1376640:CDS 45.0%
TCCACTGCATGATGTACCTT+CGG - tig0019825:125725-125744 Msa1376640:CDS 45.0%
GAGCTGAAGAAGTAGGAAGT+TGG - tig0019825:125762-125781 Msa1376640:CDS 45.0%
CTAACATGCATGGCAAAGGA+AGG - tig0019825:125846-125865 Msa1376640:CDS 45.0%
!! GGTAGCACCGACAATATTCT+AGG - tig0019825:125878-125897 Msa1376640:CDS 45.0%
TCGATGAAACGTGCATTGCT+AGG - tig0019825:125901-125920 Msa1376640:CDS 45.0%
GCTCCTCATAGCAAAAGCAA+GGG - tig0019825:125923-125942 Msa1376640:CDS 45.0%
!! TAGCCCTTGCTTTTGCTATG+AGG + tig0019825:125929-125948 None:intergenic 45.0%
TCTTTAGATGGTCTTTGGGC+AGG - tig0019825:125964-125983 Msa1376640:CDS 45.0%
!!! ACTCGTGGTTTTATCGACGG+CGG + tig0019825:123091-123110 None:intergenic 50.0%
GAGTTGGATGGGGAGACAAT+AGG - tig0019825:123247-123266 Msa1376640:three_prime_UTR 50.0%
! TAGGGTTAGCAGTCAATCGG+AGG - tig0019825:123266-123285 Msa1376640:three_prime_UTR 50.0%
TGGAAGGGCGAGTTAAAGAG+AGG + tig0019825:123365-123384 None:intergenic 50.0%
AACTCGCCCTTCCAACATAC+AGG - tig0019825:123371-123390 Msa1376640:CDS 50.0%
GACATGGCCTGTATGTTGGA+AGG + tig0019825:123381-123400 None:intergenic 50.0%
ATTGCTGACCAGAGTGGTTC+TGG - tig0019825:123435-123454 Msa1376640:CDS 50.0%
GGGTGCGAGTATAACAAGAG+TGG + tig0019825:123547-123566 None:intergenic 50.0%
CTGAGAAGCTAACTCAGCTG+TGG + tig0019825:123568-123587 None:intergenic 50.0%
! CACAGCTGAGTTAGCTTCTC+AGG - tig0019825:123566-123585 Msa1376640:intron 50.0%
ATGAGGAAGAGAAAACGGCC+AGG + tig0019825:123723-123742 None:intergenic 50.0%
TTGCAGTGCTGTCTTGGATG+AGG - tig0019825:123925-123944 Msa1376640:intron 50.0%
TGGCGATGAGGACTTCGAAA+AGG - tig0019825:123961-123980 Msa1376640:CDS 50.0%
CAAGCCCAGCCAGCAAATTT+AGG + tig0019825:124221-124240 None:intergenic 50.0%
TATGGCAGTGGCAGTACAGT+TGG - tig0019825:124342-124361 Msa1376640:intron 50.0%
! TCCGCATTGACAACAGAGAC+AGG + tig0019825:124395-124414 None:intergenic 50.0%
ACTCAATAATTGGCGCGGTG+CGG - tig0019825:124435-124454 Msa1376640:intron 50.0%
AAAAACAGGACCACCGCACT+AGG + tig0019825:124729-124748 None:intergenic 50.0%
!!! TGGGGTATTTTGGTGTGTGC+AGG - tig0019825:124825-124844 Msa1376640:intron 50.0%
ACTGGTCACTGAATCCAGCA+TGG + tig0019825:124851-124870 None:intergenic 50.0%
TGTGTCATGGCAGCATGGAA+AGG + tig0019825:125292-125311 None:intergenic 50.0%
ACTTGTAACAGAGCTTCGCG+GGG - tig0019825:125519-125538 Msa1376640:CDS 50.0%
!! TTGGAAAGGGTGCTCTCAAC+TGG - tig0019825:125799-125818 Msa1376640:CDS 50.0%
!! TGGAAAGGGTGCTCTCAACT+GGG - tig0019825:125800-125819 Msa1376640:CDS 50.0%
GGCTCCTCATAGCAAAAGCA+AGG - tig0019825:125922-125941 Msa1376640:CDS 50.0%
AAAATGGCGGTGGTTGCGCA+AGG - tig0019825:122949-122968 Msa1376640:three_prime_UTR 55.0%
! GTGGTTGCGCAAGGTGTTTG+TGG - tig0019825:122958-122977 Msa1376640:three_prime_UTR 55.0%
CGAAGTCCTCATCGCCAAAG+AGG + tig0019825:123958-123977 None:intergenic 55.0%
GTTCTCGCCTTGAAGAGGTC+AGG - tig0019825:124128-124147 Msa1376640:intron 55.0%
CTCAATAATTGGCGCGGTGC+GGG - tig0019825:124436-124455 Msa1376640:intron 55.0%
! GGGGCACTATTTCGGTAGTG+CGG - tig0019825:124462-124481 Msa1376640:intron 55.0%
!! ACTTTTGACACGCGCTGACG+CGG - tig0019825:124683-124702 Msa1376640:intron 55.0%
CGGAGTGGAGCGGAAAAAAC+AGG + tig0019825:124743-124762 None:intergenic 55.0%
AGCTGCTCTTCTGCAGCTTG+CGG - tig0019825:125053-125072 Msa1376640:CDS 55.0%
CGTGTTGGAAGAACAGCCAG+AGG - tig0019825:125612-125631 Msa1376640:CDS 55.0%
!! AAGAACAGCCAGAGGTGCCA+GGG - tig0019825:125620-125639 Msa1376640:CDS 55.0%
! GCCGAAGGTACATCATGCAG+TGG + tig0019825:125729-125748 None:intergenic 55.0%
!! TGCAGGAGTGTCATTTCCGG+TGG + tig0019825:126042-126061 None:intergenic 55.0%
GTCAATGCGGAAATGCGGCG+CGG - tig0019825:124404-124423 Msa1376640:intron 60.0%
TCCGCTCCACTCCGCTATAG+CGG - tig0019825:124749-124768 Msa1376640:intron 60.0%
! ACCGCTATAGCGGAGTGGAG+CGG + tig0019825:124753-124772 None:intergenic 60.0%
AGCCACGTCGACCGCTATAG+CGG + tig0019825:124763-124782 None:intergenic 60.0%
TTGGTGTGTGCAGGCCATGC+TGG - tig0019825:124834-124853 Msa1376640:intron 60.0%
!! GAAGAACAGCCAGAGGTGCC+AGG - tig0019825:125619-125638 Msa1376640:CDS 60.0%
!! AGAACAGCCAGAGGTGCCAG+GGG - tig0019825:125621-125640 Msa1376640:CDS 60.0%
AAATGCCTTGCCCTGTCCCC+TGG + tig0019825:125640-125659 None:intergenic 60.0%
TAATTGGCGCGGTGCGGGAG+CGG - tig0019825:124441-124460 Msa1376640:intron 65.0%
! GCGGGAGCGGGGCACTATTT+CGG - tig0019825:124454-124473 Msa1376640:intron 65.0%
! CGTCGACCGCTATAGCGGAG+TGG + tig0019825:124758-124777 None:intergenic 65.0%
CTCCGCTATAGCGGTCGACG+TGG - tig0019825:124758-124777 Msa1376640:intron 65.0%
AATTGGCGCGGTGCGGGAGC+GGG - tig0019825:124442-124461 Msa1376640:intron 70.0%
ACCACCGCACTAGGCCGAGC+CGG + tig0019825:124720-124739 None:intergenic 70.0%
!! AGCCAGAGGTGCCAGGGGAC+AGG - tig0019825:125626-125645 Msa1376640:CDS 70.0%
!! GCCAGAGGTGCCAGGGGACA+GGG - tig0019825:125627-125646 Msa1376640:CDS 70.0%
AGGTGCCAGGGGACAGGGCA+AGG - tig0019825:125632-125651 Msa1376640:CDS 70.0%
ATTGGCGCGGTGCGGGAGCG+GGG - tig0019825:124443-124462 Msa1376640:intron 75.0%
TGACGCGGCCTGAGACGCGC+CGG - tig0019825:124698-124717 Msa1376640:intron 75.0%
GCCCTGTCCCCTGGCACCTC+TGG + tig0019825:125631-125650 None:intergenic 75.0%
!!! CGGCCTGAGACGCGCCGGCT+CGG - tig0019825:124703-124722 Msa1376640:intron 80.0%
! AGGCCGAGCCGGCGCGTCTC+AGG + tig0019825:124709-124728 None:intergenic 80.0%
!!! CGCGCCGGCTCGGCCTAGTG+CGG - tig0019825:124713-124732 Msa1376640:intron 80.0%
!!! GCCGGCTCGGCCTAGTGCGG+TGG - tig0019825:124716-124735 Msa1376640:intron 80.0%
Chromosome Type Strat End Strand Name
tig0019825 gene 122884 126173 122884 ID=Msa1376640;Name=Msa1376640
tig0019825 mRNA 122884 126173 122884 ID=Msa1376640-mRNA-1;Parent=Msa1376640;Name=Msa1376640-mRNA-1;_AED=0.08;_eAED=0.08;_QI=68|1|1|1|1|1|5|412|526
tig0019825 exon 122884 123527 122884 ID=Msa1376640-mRNA-1:exon:5256;Parent=Msa1376640-mRNA-1
tig0019825 exon 123665 123840 123665 ID=Msa1376640-mRNA-1:exon:5255;Parent=Msa1376640-mRNA-1
tig0019825 exon 123941 124072 123941 ID=Msa1376640-mRNA-1:exon:5254;Parent=Msa1376640-mRNA-1
tig0019825 exon 124913 125126 124913 ID=Msa1376640-mRNA-1:exon:5253;Parent=Msa1376640-mRNA-1
tig0019825 exon 125279 126173 125279 ID=Msa1376640-mRNA-1:exon:5252;Parent=Msa1376640-mRNA-1
tig0019825 five_prime_UTR 126106 126173 126106 ID=Msa1376640-mRNA-1:five_prime_utr;Parent=Msa1376640-mRNA-1
tig0019825 CDS 125279 126105 125279 ID=Msa1376640-mRNA-1:cds;Parent=Msa1376640-mRNA-1
tig0019825 CDS 124913 125126 124913 ID=Msa1376640-mRNA-1:cds;Parent=Msa1376640-mRNA-1
tig0019825 CDS 123941 124072 123941 ID=Msa1376640-mRNA-1:cds;Parent=Msa1376640-mRNA-1
tig0019825 CDS 123665 123840 123665 ID=Msa1376640-mRNA-1:cds;Parent=Msa1376640-mRNA-1
tig0019825 CDS 123296 123527 123296 ID=Msa1376640-mRNA-1:cds;Parent=Msa1376640-mRNA-1
tig0019825 three_prime_UTR 122884 123295 122884 ID=Msa1376640-mRNA-1:three_prime_utr;Parent=Msa1376640-mRNA-1
Gene Sequence

>Msa1376640

ATGGCGGTGGTTGCGCAAGGTGTTTGTGGTAAAGCGTTGCTGTTTAGCATCGGAGTTGGAGTGAGACCCAGAAGACCCACTAAACACTCTGTCTTCTTCTTCGTTTCTAATTCAAAATCCAATTCAAATTCAAAATCCGCCGTCGATAAAACCACGAGTTTCGGAAGACTAAAAGTTGAGAAAGTGAATGCTCTCATCCACAGAACCAAGCAAAATACCACAAAACGCAAACAACTACAACTAGAAGATGAAGATGAAGACAACAATTCTGCAACTGCAACTGCAACTCATTCGAGAGTTGGATGGGGAGACAATAGGGTTAGCAGTCAATCGGAGGACACCAACTTTTTCAGTCTAAAATCATTTAAAGACATTGGATGCGCCGATTTCATGATTGAATCTCTCCATAACCTCTCTTTAACTCGCCCTTCCAACATACAGGCCATGTCATTTGCTCCTGTTATTGCCGGAAAGTCTTGTATAATTGCTGACCAGAGTGGTTCTGGAAAGACATTAGCTTATCTTGTACCAATCATTCAACGTCTCAGACAACAAGAACTACAAGGACTTCATAAATCTTCTTCACGATCTCCCACTCTTGTTATACTCGCACCCACAGCTGAGTTAGCTTCTCAGGTCTTCCATAATTGTCGTTCAATGTCTAAATCTGGCAAGGTTCCATTCAAATCTATGCTTGTCACTGGTGGCTTCCGACAAAAAACTCAACTCGACACTTTAAACCAGGGTGTTGATGTCTTCATAGCTACACCTGGCCGTTTTCTCTTCCTCATCAACCAAGGCTTTTTGCACTTAACTAATCTAACATGTGCTGTCTTGGATGAGGTTGATATCCTCTTTGGCGATGAGGACTTCGAAAAGGCTCTTCAATGCTTGATTAGTTCTTCACCGGTGGACACACAATATTTATTTGTAACTGCTACTTTACCAAAAAATGTTTACAGCAAACTGGTTGAAGTTTTTCCTGATTGTGAAATGGTCATGGGACCTAGTATGCATCGAATAAGTTCTCGCCTTGAAGAGATAATAGTAGATTGTAGTGGGGAAGATGGACAAGAAAAAACTCTCGACACAGCATTTTTGAACAAGAAAGCTGCTCTTCTGCAGCTTGCGGAGAAGAATCGCGTCCCAAGAACTATTGTGTTCTGCAACAAAATTGAAACATGCAGAAAAGTTGAAAATGCATTAAACCGTTTTGATAGAAAGGGAATTCGCATGCAAGTTCTACCTTTCCATGCTGCCATGACACAAGAATCACGGCTTACTAGTATGAAGGAGTTTACACGTTCTCCACCAAAAGAAGTGTCCCAGTTTATGGTTTGCACGGACAGAGCTTCGCGGGGAATTGACTTCACTAGAGTGGATCATGTAATACTTTTTGACTACCCACGTGATCCAAGTGAATATGTACGGCGTGTTGGAAGAACAGCCAGAGGTGCCAGGGGACAGGGCAAGGCATTTATCTTTGTTGTTGGCAAGCAAGTATCCCTTGCACAAAAAGTAATGGAAAGAAATCGGAAGGGTCATCCACTGCATGATGTACCTTCGGCTTATGAGTATTGA
Protein sequence

>Msa1376640

MAVVAQGVCGKALLFSIGVGVRPRRPTKHSVFFFVSNSKSNSNSKSAVDKTTSFGRLKVEKVNALIHRTKQNTTKRKQLQLEDEDEDNNSATATATHSRVGWGDNRVSSQSEDTNFFSLKSFKDIGCADFMIESLHNLSLTRPSNIQAMSFAPVIAGKSCIIADQSGSGKTLAYLVPIIQRLRQQELQGLHKSSSRSPTLVILAPTAELASQVFHNCRSMSKSGKVPFKSMLVTGGFRQKTQLDTLNQGVDVFIATPGRFLFLINQGFLHLTNLTCAVLDEVDILFGDEDFEKALQCLISSSPVDTQYLFVTATLPKNVYSKLVEVFPDCEMVMGPSMHRISSRLEEIIVDCSGEDGQEKTLDTAFLNKKAALLQLAEKNRVPRTIVFCNKIETCRKVENALNRFDRKGIRMQVLPFHAAMTQESRLTSMKEFTRSPPKEVSQFMVCTDRASRGIDFTRVDHVILFDYPRDPSEYVRRVGRTARGARGQGKAFIFVVGKQVSLAQKVMERNRKGHPLHDVPSAYEY*