Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0594150 | KEH29195.1 | 84.906 | 106 | 16 | 0 | 1 | 106 | 58 | 163 | 2.87e-57 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0594150 | sp|A0AT31|NLTP5_LENCU | 76.923 | 104 | 24 | 0 | 3 | 106 | 4 | 107 | 9.58e-52 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0594150 | A0A072UTH7 | 84.906 | 106 | 16 | 0 | 1 | 106 | 58 | 163 | 1.37e-57 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010460 | Msa0594150 | -0.806024 | 1.005500e-49 | -8.615850e-47 |
| Msa0041540 | Msa0594150 | 0.805738 | 1.154937e-49 | -8.615850e-47 |
| Msa0057260 | Msa0594150 | 0.802875 | 4.557401e-49 | -8.615850e-47 |
| Msa0059960 | Msa0594150 | -0.819166 | 1.339172e-52 | -8.615850e-47 |
| Msa0065390 | Msa0594150 | 0.806903 | 6.560136e-50 | -8.615850e-47 |
| Msa0072820 | Msa0594150 | 0.826982 | 2.012029e-54 | -8.615850e-47 |
| Msa0075850 | Msa0594150 | 0.819515 | 1.115382e-52 | -8.615850e-47 |
| Msa0094060 | Msa0594150 | 0.800629 | 1.317660e-48 | -8.615850e-47 |
| Msa0103780 | Msa0594150 | 0.808456 | 3.069491e-50 | -8.615850e-47 |
| Msa0110710 | Msa0594150 | 0.813142 | 2.972497e-51 | -8.615850e-47 |
| Msa0139740 | Msa0594150 | 0.806575 | 7.695486e-50 | -8.615850e-47 |
| Msa0141670 | Msa0594150 | 0.816219 | 6.190406e-52 | -8.615850e-47 |
| Msa0150210 | Msa0594150 | 0.808372 | 3.199718e-50 | -8.615850e-47 |
| Msa0157020 | Msa0594150 | 0.833205 | 6.094735e-56 | -8.615850e-47 |
| Msa0185550 | Msa0594150 | 0.800320 | 1.523449e-48 | -8.615850e-47 |
| Msa0207180 | Msa0594150 | 0.807951 | 3.932001e-50 | -8.615850e-47 |
| Msa0224700 | Msa0594150 | 0.812165 | 4.863188e-51 | -8.615850e-47 |
| Msa0230400 | Msa0594150 | 0.843520 | 1.331933e-58 | -8.615850e-47 |
| Msa0380420 | Msa0594150 | 0.810432 | 1.156271e-50 | -8.615850e-47 |
| Msa0392450 | Msa0594150 | 0.812766 | 3.594355e-51 | -8.615850e-47 |
| Msa0392480 | Msa0594150 | 0.800167 | 1.636638e-48 | -8.615850e-47 |
| Msa0421790 | Msa0594150 | 0.829033 | 6.455214e-55 | -8.615850e-47 |
| Msa0429270 | Msa0594150 | 0.823942 | 1.055304e-53 | -8.615850e-47 |
| Msa0439780 | Msa0594150 | 0.802198 | 6.286603e-49 | -8.615850e-47 |
| Msa0467890 | Msa0594150 | 0.806421 | 8.294423e-50 | -8.615850e-47 |
| Msa0467970 | Msa0594150 | 0.806213 | 9.174842e-50 | -8.615850e-47 |
| Msa0477780 | Msa0594150 | 0.833058 | 6.630989e-56 | -8.615850e-47 |
| Msa0253410 | Msa0594150 | 0.806469 | 8.101241e-50 | -8.615850e-47 |
| Msa0274780 | Msa0594150 | 0.854163 | 1.478423e-61 | -8.615850e-47 |
| Msa0303780 | Msa0594150 | 0.807917 | 3.998844e-50 | -8.615850e-47 |
| Msa0315480 | Msa0594150 | 0.816404 | 5.626636e-52 | -8.615850e-47 |
| Msa0487560 | Msa0594150 | 0.805033 | 1.623047e-49 | -8.615850e-47 |
| Msa0487620 | Msa0594150 | 0.814019 | 1.906593e-51 | -8.615850e-47 |
| Msa0504740 | Msa0594150 | 0.800716 | 1.264692e-48 | -8.615850e-47 |
| Msa0537870 | Msa0594150 | 0.870987 | 9.627762e-67 | -8.615850e-47 |
| Msa0537880 | Msa0594150 | 0.842206 | 2.978155e-58 | -8.615850e-47 |
| Msa0538260 | Msa0594150 | 0.837774 | 4.261256e-57 | -8.615850e-47 |
| Msa0538570 | Msa0594150 | 0.816458 | 5.474015e-52 | -8.615850e-47 |
| Msa0558850 | Msa0594150 | 0.808887 | 2.482863e-50 | -8.615850e-47 |
| Msa0594140 | Msa0594150 | 0.811052 | 8.491137e-51 | -8.615850e-47 |
| Msa0594150 | Msa0594180 | 0.892534 | 1.453175e-74 | -8.615850e-47 |
| Msa0594150 | Msa0594390 | 0.831556 | 1.561430e-55 | -8.615850e-47 |
| Msa0594150 | Msa0600390 | 0.825026 | 5.866053e-54 | -8.615850e-47 |
| Msa0594150 | Msa0612150 | 0.809710 | 1.654391e-50 | -8.615850e-47 |
| Msa0594150 | Msa0633860 | 0.828919 | 6.878111e-55 | -8.615850e-47 |
| Msa0594150 | Msa0634270 | 0.807229 | 5.597941e-50 | -8.615850e-47 |
| Msa0594150 | Msa0640390 | 0.802357 | 5.830889e-49 | -8.615850e-47 |
| Msa0594150 | Msa0640880 | 0.828975 | 6.666578e-55 | -8.615850e-47 |
| Msa0594150 | Msa0659070 | 0.803205 | 3.895268e-49 | -8.615850e-47 |
| Msa0594150 | Msa0723150 | 0.810262 | 1.258170e-50 | -8.615850e-47 |
| Msa0594150 | Msa0803130 | 0.819916 | 9.031246e-53 | -8.615850e-47 |
| Msa0594150 | Msa0819470 | 0.803659 | 3.137428e-49 | -8.615850e-47 |
| Msa0594150 | Msa0841490 | 0.815361 | 9.614805e-52 | -8.615850e-47 |
| Msa0594150 | Msa0859390 | 0.800248 | 1.575814e-48 | -8.615850e-47 |
| Msa0594150 | Msa0875360 | 0.824460 | 7.975769e-54 | -8.615850e-47 |
| Msa0594150 | Msa0931480 | 0.817022 | 4.090490e-52 | -8.615850e-47 |
| Msa0594150 | Msa0955310 | 0.805569 | 1.252992e-49 | -8.615850e-47 |
| Msa0594150 | Msa1004500 | 0.801147 | 1.032960e-48 | -8.615850e-47 |
| Msa0594150 | Msa1134400 | 0.804446 | 2.151493e-49 | -8.615850e-47 |
| Msa0594150 | Msa1200920 | 0.827353 | 1.639822e-54 | -8.615850e-47 |
| Msa0594150 | Msa1208510 | 0.857027 | 2.159210e-62 | -8.615850e-47 |
| Msa0594150 | Msa1219250 | 0.804925 | 1.709565e-49 | -8.615850e-47 |
| Msa0594150 | Msa1232860 | 0.817909 | 2.581718e-52 | -8.615850e-47 |
| Msa0594150 | Msa1258810 | 0.822338 | 2.498623e-53 | -8.615850e-47 |
| Msa0594150 | Msa1274740 | 0.805048 | 1.611279e-49 | -8.615850e-47 |
| Msa0594150 | Msa1275290 | 0.825762 | 3.928197e-54 | -8.615850e-47 |
| Msa0594150 | Msa1312730 | 0.806142 | 9.497187e-50 | -8.615850e-47 |
| Msa0594150 | Msa1323060 | 0.800316 | 1.525955e-48 | -8.615850e-47 |
| Msa0594150 | Msa1330240 | 0.818424 | 1.974341e-52 | -8.615850e-47 |
| Msa0594150 | Msa1365210 | 0.837045 | 6.553105e-57 | -8.615850e-47 |
| Msa0594150 | Msa1367940 | 0.813518 | 2.458233e-51 | -8.615850e-47 |
| Msa0594150 | Msa1369860 | 0.851581 | 8.085769e-61 | -8.615850e-47 |
| Msa0594150 | Msa1375630 | 0.809483 | 1.850671e-50 | -8.615850e-47 |
| Msa0594150 | Msa1376530 | 0.820734 | 5.865252e-53 | -8.615850e-47 |
| Msa0594150 | Msa1405770 | 0.811548 | 6.627372e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0594150 | MtrunA17_Chr4g0012951 | 84.906 | 106 | 16 | 0 | 1 | 106 | 1 | 106 | 6.87e-61 | 182 |
| Msa0594150 | MtrunA17_Chr4g0012991 | 84.906 | 106 | 16 | 0 | 1 | 106 | 56 | 161 | 2.25e-59 | 181 |
| Msa0594150 | MtrunA17_Chr4g0013001 | 83.962 | 106 | 17 | 0 | 1 | 106 | 1 | 106 | 5.17e-59 | 177 |
| Msa0594150 | MtrunA17_Chr4g0012961 | 83.019 | 106 | 18 | 0 | 1 | 106 | 1 | 106 | 2.22e-58 | 176 |
| Msa0594150 | MtrunA17_Chr4g0013261 | 65.741 | 108 | 34 | 2 | 1 | 106 | 1 | 107 | 6.75e-43 | 137 |
| Msa0594150 | MtrunA17_Chr4g0013251 | 65.741 | 108 | 34 | 2 | 1 | 106 | 1 | 107 | 8.73e-42 | 134 |
| Msa0594150 | MtrunA17_Chr4g0012901 | 50.909 | 110 | 50 | 3 | 1 | 108 | 5 | 112 | 5.88e-30 | 104 |
| Msa0594150 | MtrunA17_Chr4g0013211 | 45.455 | 110 | 60 | 0 | 1 | 110 | 1 | 110 | 3.25e-28 | 100 |
| Msa0594150 | MtrunA17_Chr7g0244691 | 45.872 | 109 | 56 | 2 | 4 | 110 | 7 | 114 | 2.93e-27 | 97.8 |
| Msa0594150 | MtrunA17_Chr7g0244831 | 45.133 | 113 | 57 | 4 | 2 | 110 | 3 | 114 | 1.15e-25 | 93.6 |
| Msa0594150 | MtrunA17_Chr7g0244841 | 43.119 | 109 | 58 | 3 | 4 | 110 | 7 | 113 | 6.35e-25 | 91.7 |
| Msa0594150 | MtrunA17_Chr7g0244641 | 40.367 | 109 | 63 | 2 | 4 | 110 | 7 | 115 | 8.20e-25 | 91.7 |
| Msa0594150 | MtrunA17_Chr4g0012891 | 44.037 | 109 | 54 | 3 | 5 | 106 | 9 | 117 | 1.38e-24 | 90.9 |
| Msa0594150 | MtrunA17_Chr7g0244671 | 40.000 | 105 | 61 | 1 | 4 | 106 | 7 | 111 | 2.01e-24 | 91.3 |
| Msa0594150 | MtrunA17_Chr2g0309241 | 42.991 | 107 | 59 | 2 | 1 | 106 | 1 | 106 | 7.65e-23 | 86.3 |
| Msa0594150 | MtrunA17_Chr4g0012931 | 40.741 | 108 | 61 | 1 | 1 | 105 | 1 | 108 | 7.83e-22 | 84.3 |
| Msa0594150 | MtrunA17_Chr7g0244811 | 36.134 | 119 | 72 | 2 | 1 | 117 | 5 | 121 | 1.11e-21 | 83.6 |
| Msa0594150 | MtrunA17_Chr7g0244741 | 40.000 | 110 | 63 | 1 | 4 | 110 | 7 | 116 | 9.54e-19 | 75.9 |
| Msa0594150 | MtrunA17_Chr7g0244681 | 43.396 | 106 | 55 | 4 | 4 | 106 | 7 | 110 | 4.86e-18 | 73.9 |
| Msa0594150 | MtrunA17_Chr4g0012921 | 40.367 | 109 | 62 | 2 | 1 | 106 | 1 | 109 | 1.04e-17 | 73.2 |
| Msa0594150 | MtrunA17_Chr3g0107481 | 38.235 | 102 | 62 | 1 | 5 | 105 | 3 | 104 | 4.17e-17 | 71.6 |
| Msa0594150 | MtrunA17_Chr7g0233431 | 31.193 | 109 | 74 | 1 | 5 | 112 | 20 | 128 | 6.36e-16 | 68.9 |
| Msa0594150 | MtrunA17_Chr7g0244721 | 34.862 | 109 | 69 | 1 | 4 | 110 | 7 | 115 | 1.31e-15 | 67.8 |
| Msa0594150 | MtrunA17_Chr7g0244761 | 37.615 | 109 | 65 | 3 | 4 | 110 | 32 | 139 | 4.78e-15 | 67.0 |
| Msa0594150 | MtrunA17_Chr7g0244821 | 37.615 | 109 | 64 | 4 | 4 | 110 | 7 | 113 | 5.89e-15 | 66.2 |
| Msa0594150 | MtrunA17_Chr1g0165761 | 40.741 | 108 | 61 | 2 | 5 | 110 | 8 | 114 | 8.64e-15 | 65.9 |
| Msa0594150 | MtrunA17_Chr7g0244791 | 36.036 | 111 | 64 | 2 | 4 | 110 | 12 | 119 | 1.49e-14 | 65.5 |
| Msa0594150 | MtrunA17_Chr3g0105001 | 36.111 | 108 | 61 | 4 | 4 | 104 | 7 | 113 | 4.47e-14 | 63.9 |
| Msa0594150 | MtrunA17_Chr7g0244661 | 38.739 | 111 | 60 | 3 | 4 | 112 | 7 | 111 | 6.87e-14 | 63.2 |
| Msa0594150 | MtrunA17_Chr3g0104971 | 36.449 | 107 | 60 | 4 | 4 | 103 | 7 | 112 | 9.30e-14 | 63.2 |
| Msa0594150 | MtrunA17_Chr3g0105021 | 38.235 | 102 | 55 | 4 | 9 | 103 | 12 | 112 | 9.60e-14 | 63.2 |
| Msa0594150 | MtrunA17_Chr3g0105011 | 38.235 | 102 | 55 | 4 | 9 | 103 | 12 | 112 | 9.60e-14 | 63.2 |
| Msa0594150 | MtrunA17_Chr3g0104981 | 36.449 | 107 | 60 | 4 | 4 | 103 | 7 | 112 | 9.69e-14 | 63.2 |
| Msa0594150 | MtrunA17_Chr3g0105031 | 36.449 | 107 | 60 | 4 | 9 | 108 | 2 | 107 | 2.42e-13 | 61.6 |
| Msa0594150 | MtrunA17_Chr3g0104991 | 38.235 | 102 | 55 | 4 | 9 | 103 | 12 | 112 | 2.46e-13 | 62.0 |
| Msa0594150 | MtrunA17_Chr3g0104951 | 35.514 | 107 | 61 | 4 | 4 | 103 | 7 | 112 | 2.63e-13 | 62.0 |
| Msa0594150 | MtrunA17_Chr3g0104931 | 35.514 | 107 | 61 | 4 | 4 | 103 | 7 | 112 | 2.63e-13 | 62.0 |
| Msa0594150 | MtrunA17_Chr3g0104941 | 35.514 | 107 | 61 | 4 | 4 | 103 | 7 | 112 | 2.63e-13 | 62.0 |
| Msa0594150 | MtrunA17_Chr3g0104921 | 36.893 | 103 | 57 | 4 | 9 | 104 | 2 | 103 | 3.35e-13 | 61.6 |
| Msa0594150 | MtrunA17_Chr3g0104911 | 36.893 | 103 | 57 | 4 | 9 | 104 | 2 | 103 | 3.35e-13 | 61.6 |
| Msa0594150 | MtrunA17_Chr3g0104901 | 35.398 | 113 | 62 | 5 | 1 | 103 | 1 | 112 | 4.39e-13 | 61.6 |
| Msa0594150 | MtrunA17_Chr3g0095991 | 35.238 | 105 | 62 | 4 | 3 | 103 | 10 | 112 | 8.23e-13 | 60.8 |
| Msa0594150 | MtrunA17_Chr3g0105041 | 35.514 | 107 | 61 | 4 | 4 | 103 | 7 | 112 | 8.98e-13 | 60.5 |
| Msa0594150 | MtrunA17_Chr3g0104961 | 35.514 | 107 | 61 | 4 | 4 | 103 | 7 | 112 | 1.84e-12 | 59.7 |
| Msa0594150 | MtrunA17_Chr7g0244751 | 33.333 | 108 | 71 | 1 | 4 | 110 | 7 | 114 | 2.22e-12 | 59.7 |
| Msa0594150 | MtrunA17_Chr3g0101431 | 36.275 | 102 | 57 | 4 | 9 | 103 | 11 | 111 | 3.72e-12 | 58.9 |
| Msa0594150 | MtrunA17_Chr3g0095951 | 37.349 | 83 | 47 | 3 | 25 | 103 | 31 | 112 | 7.59e-12 | 58.2 |
| Msa0594150 | MtrunA17_Chr3g0096021 | 37.349 | 83 | 47 | 3 | 25 | 103 | 31 | 112 | 9.10e-12 | 58.2 |
| Msa0594150 | MtrunA17_Chr3g0096031 | 37.349 | 83 | 47 | 3 | 25 | 103 | 31 | 112 | 1.25e-11 | 57.8 |
| Msa0594150 | MtrunA17_Chr3g0094521 | 29.808 | 104 | 67 | 4 | 10 | 110 | 18 | 118 | 2.67e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0594150 | AT5G59320.1 | 58.000 | 100 | 40 | 1 | 8 | 107 | 10 | 107 | 9.79e-35 | 117 |
| Msa0594150 | AT2G38540.1 | 61.682 | 107 | 35 | 6 | 4 | 106 | 5 | 109 | 1.30e-31 | 108 |
| Msa0594150 | AT5G59310.1 | 55.556 | 99 | 39 | 2 | 8 | 106 | 10 | 103 | 4.62e-30 | 104 |
| Msa0594150 | AT2G38530.1 | 59.434 | 106 | 39 | 4 | 4 | 106 | 5 | 109 | 2.07e-28 | 100 |
| Msa0594150 | AT2G15050.2 | 44.144 | 111 | 58 | 3 | 4 | 110 | 5 | 115 | 3.14e-26 | 95.1 |
| Msa0594150 | AT2G15050.3 | 44.144 | 111 | 58 | 3 | 4 | 110 | 5 | 115 | 4.39e-26 | 95.1 |
| Msa0594150 | AT2G15050.1 | 44.144 | 111 | 58 | 3 | 4 | 110 | 5 | 115 | 6.67e-26 | 94.7 |
| Msa0594150 | AT3G51600.1 | 52.830 | 106 | 46 | 4 | 4 | 106 | 5 | 109 | 2.17e-25 | 93.2 |
| Msa0594150 | AT3G51590.1 | 41.964 | 112 | 62 | 3 | 6 | 116 | 8 | 117 | 2.16e-24 | 90.9 |
| Msa0594150 | AT4G33355.1 | 52.326 | 86 | 39 | 1 | 24 | 107 | 28 | 113 | 3.68e-23 | 87.4 |
| Msa0594150 | AT4G33355.2 | 52.326 | 86 | 39 | 1 | 24 | 107 | 28 | 113 | 4.43e-23 | 87.4 |
| Msa0594150 | AT5G01870.1 | 44.231 | 104 | 50 | 4 | 9 | 105 | 4 | 106 | 3.10e-21 | 82.4 |
| Msa0594150 | AT3G08770.1 | 44.318 | 88 | 45 | 3 | 22 | 106 | 18 | 104 | 6.12e-17 | 71.6 |
| Msa0594150 | AT3G08770.2 | 44.318 | 88 | 45 | 3 | 22 | 106 | 18 | 104 | 6.91e-17 | 71.6 |
| Msa0594150 | AT2G18370.1 | 40.449 | 89 | 51 | 2 | 15 | 102 | 16 | 103 | 5.34e-16 | 69.3 |
Find 47 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTTATTGGTGAAATATTT+TGG | 0.077015 | 4_2:-21241289 | Msa0594150:three_prime_UTR |
| AGTGCTGCCGGTGCTATTTC+TGG | 0.185000 | 4_2:-21242052 | Msa0594150:CDS |
| TAAGTGAAAGGAAATAAAAT+TGG | 0.248080 | 4_2:-21241130 | Msa0594150:three_prime_UTR |
| GTTGCAGCTACAAATTTATA+TGG | 0.264074 | 4_2:-21241458 | Msa0594150:CDS |
| TTATTCTTAATAGCATTAAA+TGG | 0.274471 | 4_2:+21241409 | None:intergenic |
| CCATGCATTGCATATCTTAG+AGG | 0.320070 | 4_2:-21242175 | Msa0594150:CDS |
| TGGTTATATTTACAGCATTA+GGG | 0.341414 | 4_2:-21241493 | Msa0594150:intron |
| AAGTGAAAGGAAATAAAATT+GGG | 0.366480 | 4_2:-21241129 | Msa0594150:three_prime_UTR |
| CTTCTTATACCAGACTGTAT+TGG | 0.369553 | 4_2:-21241345 | Msa0594150:three_prime_UTR |
| CCATCTCCAGCATGCTGTGC+TGG | 0.375274 | 4_2:-21242142 | Msa0594150:CDS |
| TTGCAAGCAGCCTGACGGTC+AGG | 0.421711 | 4_2:+21242085 | None:intergenic |
| GGTAGAGGTACTGATCTTGT+AGG | 0.430936 | 4_2:+21241976 | None:intergenic |
| CACCACCACGCCTGACCGTC+AGG | 0.443141 | 4_2:-21242095 | Msa0594150:CDS |
| ACGGTCAGGCGTGGTGGTGG+CGG | 0.445777 | 4_2:+21242099 | None:intergenic |
| GTCAGGCGTGGTGGTGGCGG+CGG | 0.446691 | 4_2:+21242102 | None:intergenic |
| GTGCATGATTGTTGCACCTA+TGG | 0.467928 | 4_2:-21242242 | Msa0594150:CDS |
| CAGTCTGGTATAAGAAGGAT+AGG | 0.473305 | 4_2:+21241351 | None:intergenic |
| TTCACTCCAGCACAGCATGC+TGG | 0.485039 | 4_2:+21242136 | None:intergenic |
| ATGTTAACACCACATTTGCC+AGG | 0.490963 | 4_2:+21242001 | None:intergenic |
| AACACAGCTGCTGGTCTCCC+TGG | 0.491804 | 4_2:-21242019 | Msa0594150:CDS |
| ATCTCATGTGGACAAGTAAC+TGG | 0.494105 | 4_2:-21242208 | Msa0594150:CDS |
| GCATATCTTAGAGGTGGTGC+TGG | 0.504129 | 4_2:-21242166 | Msa0594150:CDS |
| GCTGGTCTCCCTGGCAAATG+TGG | 0.511355 | 4_2:-21242010 | Msa0594150:CDS |
| CTGACGGTCAGGCGTGGTGG+TGG | 0.517462 | 4_2:+21242096 | None:intergenic |
| AACTGCTTGAAAAGTGCTGC+CGG | 0.524263 | 4_2:-21242064 | Msa0594150:CDS |
| AAGAATAATAATGAGACTGA+TGG | 0.524929 | 4_2:-21241394 | Msa0594150:three_prime_UTR |
| CAATACAGTCTGGTATAAGA+AGG | 0.533082 | 4_2:+21241346 | None:intergenic |
| CCAGCACAGCATGCTGGAGA+TGG | 0.550113 | 4_2:+21242142 | None:intergenic |
| TTGAATGCTAACACAGCTGC+TGG | 0.551046 | 4_2:-21242028 | Msa0594150:CDS |
| TCTATCTACTAATAAGTGAA+AGG | 0.551595 | 4_2:-21241142 | Msa0594150:three_prime_UTR |
| GTAGAGGTACTGATCTTGTA+GGG | 0.565695 | 4_2:+21241977 | None:intergenic |
| AGCAGCCTGACGGTCAGGCG+TGG | 0.565783 | 4_2:+21242090 | None:intergenic |
| TAAGACAATCCAATACAGTC+TGG | 0.567330 | 4_2:+21241336 | None:intergenic |
| AGCCTGACGGTCAGGCGTGG+TGG | 0.576862 | 4_2:+21242093 | None:intergenic |
| GAGATTGCAGCATCTGCCAT+AGG | 0.577723 | 4_2:+21242226 | None:intergenic |
| CCTCTAAGATATGCAATGCA+TGG | 0.588805 | 4_2:+21242175 | None:intergenic |
| AGTGAAAGGAAATAAAATTG+GGG | 0.602850 | 4_2:-21241128 | Msa0594150:three_prime_UTR |
| TGCATTGCATATCTTAGAGG+TGG | 0.623803 | 4_2:-21242172 | Msa0594150:CDS |
| AGCAGTTGCAAGCAGCCTGA+CGG | 0.624471 | 4_2:+21242080 | None:intergenic |
| AATAATAATGAGACTGATGG+AGG | 0.629956 | 4_2:-21241391 | Msa0594150:three_prime_UTR |
| ATGGAGGCTCATCTTATGTG+TGG | 0.631112 | 4_2:-21241375 | Msa0594150:three_prime_UTR |
| GGCACTCTCATATACTAGTG+AGG | 0.641835 | 4_2:+21241430 | None:intergenic |
| TGTTAACACCACATTTGCCA+GGG | 0.642773 | 4_2:+21242002 | None:intergenic |
| ATAAGAGTAATGACATAACC+AGG | 0.643003 | 4_2:+21241259 | None:intergenic |
| ATTCAAACCAGAAATAGCAC+CGG | 0.646340 | 4_2:+21242045 | None:intergenic |
| AGTCTGGTATAAGAAGGATA+GGG | 0.652754 | 4_2:+21241352 | None:intergenic |
| GCAGATGCTGCAATCTCATG+TGG | 0.654132 | 4_2:-21242220 | Msa0594150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATAATAATTTTTTTTTT+AGG | - | chr4_2:21241734-21241753 | Msa0594150:intron | 0.0% |
| !!! | TTTATAATAATTTTTTTTTT+AGG | - | chr4_2:21241734-21241753 | Msa0594150:intron | 0.0% |
| !!! | ATTTTTGTATGTTTATTATT+GGG | + | chr4_2:21241596-21241615 | None:intergenic | 10.0% |
| !!! | AATTTTTGTATGTTTATTAT+TGG | + | chr4_2:21241597-21241616 | None:intergenic | 10.0% |
| !!! | TTTATTGGTTATTTATTTTT+TGG | - | chr4_2:21241787-21241806 | Msa0594150:intron | 10.0% |
| !!! | GTTTTTTTTTTTTTTTTGTT+TGG | - | chr4_2:21241866-21241885 | Msa0594150:intron | 10.0% |
| !!! | ATTTTTGTATGTTTATTATT+GGG | + | chr4_2:21241596-21241615 | None:intergenic | 10.0% |
| !!! | AATTTTTGTATGTTTATTAT+TGG | + | chr4_2:21241597-21241616 | None:intergenic | 10.0% |
| !!! | TTTATTGGTTATTTATTTTT+TGG | - | chr4_2:21241787-21241806 | Msa0594150:intron | 10.0% |
| !!! | GTTTTTTTTTTTTTTTTGTT+TGG | - | chr4_2:21241866-21241885 | Msa0594150:intron | 10.0% |
| !! | TATTAACATAAAAGACTAAT+TGG | - | chr4_2:21241616-21241635 | Msa0594150:intron | 15.0% |
| !!! | AAACATTTTTGTGAATTTTT+TGG | - | chr4_2:21241704-21241723 | Msa0594150:intron | 15.0% |
| !!! | TTATTTTTTGGTGTTATTAT+TGG | - | chr4_2:21241799-21241818 | Msa0594150:intron | 15.0% |
| !! | TTATTCTTAATAGCATTAAA+TGG | + | chr4_2:21241973-21241992 | None:intergenic | 15.0% |
| !! | TATTAACATAAAAGACTAAT+TGG | - | chr4_2:21241616-21241635 | Msa0594150:intron | 15.0% |
| !!! | AAACATTTTTGTGAATTTTT+TGG | - | chr4_2:21241704-21241723 | Msa0594150:intron | 15.0% |
| !!! | TTATTTTTTGGTGTTATTAT+TGG | - | chr4_2:21241799-21241818 | Msa0594150:intron | 15.0% |
| !! | TTATTCTTAATAGCATTAAA+TGG | + | chr4_2:21241973-21241992 | None:intergenic | 15.0% |
| !!! | TTATTGGTTAATATTCAAGT+CGG | - | chr4_2:21241815-21241834 | Msa0594150:intron | 20.0% |
| !!! | TTGTTTTATGAGTTACTTAT+TGG | - | chr4_2:21242077-21242096 | Msa0594150:CDS | 20.0% |
| !!! | TACTTATTGGTGAAATATTT+TGG | - | chr4_2:21242090-21242109 | Msa0594150:CDS | 20.0% |
| !!! | ACTTATTGGTGAAATATTTT+GGG | - | chr4_2:21242091-21242110 | Msa0594150:CDS | 20.0% |
| !! | TAAGTGAAAGGAAATAAAAT+TGG | - | chr4_2:21242249-21242268 | Msa0594150:CDS | 20.0% |
| !! | AAGTGAAAGGAAATAAAATT+GGG | - | chr4_2:21242250-21242269 | Msa0594150:CDS | 20.0% |
| !!! | TTATTGGTTAATATTCAAGT+CGG | - | chr4_2:21241815-21241834 | Msa0594150:intron | 20.0% |
| !!! | TTGTTTTATGAGTTACTTAT+TGG | - | chr4_2:21242077-21242096 | Msa0594150:CDS | 20.0% |
| !!! | TACTTATTGGTGAAATATTT+TGG | - | chr4_2:21242090-21242109 | Msa0594150:CDS | 20.0% |
| !!! | ACTTATTGGTGAAATATTTT+GGG | - | chr4_2:21242091-21242110 | Msa0594150:CDS | 20.0% |
| !! | TAAGTGAAAGGAAATAAAAT+TGG | - | chr4_2:21242249-21242268 | Msa0594150:CDS | 20.0% |
| !! | AAGTGAAAGGAAATAAAATT+GGG | - | chr4_2:21242250-21242269 | Msa0594150:CDS | 20.0% |
| ! | TTGGTTATATTTACAGCATT+AGG | - | chr4_2:21241885-21241904 | Msa0594150:intron | 25.0% |
| ! | TGGTTATATTTACAGCATTA+GGG | - | chr4_2:21241886-21241905 | Msa0594150:intron | 25.0% |
| ! | AAGAATAATAATGAGACTGA+TGG | - | chr4_2:21241985-21242004 | Msa0594150:intron | 25.0% |
| !!! | AAATATTTTGGGTTTTTACC+TGG | - | chr4_2:21242102-21242121 | Msa0594150:CDS | 25.0% |
| ! | TCTATCTACTAATAAGTGAA+AGG | - | chr4_2:21242237-21242256 | Msa0594150:CDS | 25.0% |
| ! | AGTGAAAGGAAATAAAATTG+GGG | - | chr4_2:21242251-21242270 | Msa0594150:CDS | 25.0% |
| ! | TTGGTTATATTTACAGCATT+AGG | - | chr4_2:21241885-21241904 | Msa0594150:intron | 25.0% |
| ! | TGGTTATATTTACAGCATTA+GGG | - | chr4_2:21241886-21241905 | Msa0594150:intron | 25.0% |
| ! | AAGAATAATAATGAGACTGA+TGG | - | chr4_2:21241985-21242004 | Msa0594150:intron | 25.0% |
| !!! | AAATATTTTGGGTTTTTACC+TGG | - | chr4_2:21242102-21242121 | Msa0594150:CDS | 25.0% |
| ! | TCTATCTACTAATAAGTGAA+AGG | - | chr4_2:21242237-21242256 | Msa0594150:CDS | 25.0% |
| ! | AGTGAAAGGAAATAAAATTG+GGG | - | chr4_2:21242251-21242270 | Msa0594150:CDS | 25.0% |
| GTTGCAGCTACAAATTTATA+TGG | - | chr4_2:21241921-21241940 | Msa0594150:intron | 30.0% | |
| AATAATAATGAGACTGATGG+AGG | - | chr4_2:21241988-21242007 | Msa0594150:CDS | 30.0% | |
| ATAAGAGTAATGACATAACC+AGG | + | chr4_2:21242123-21242142 | None:intergenic | 30.0% | |
| GTTGCAGCTACAAATTTATA+TGG | - | chr4_2:21241921-21241940 | Msa0594150:intron | 30.0% | |
| AATAATAATGAGACTGATGG+AGG | - | chr4_2:21241988-21242007 | Msa0594150:CDS | 30.0% | |
| ATAAGAGTAATGACATAACC+AGG | + | chr4_2:21242123-21242142 | None:intergenic | 30.0% | |
| ATTCAAACCAGAAATAGCAC+CGG | + | chr4_2:21241337-21241356 | None:intergenic | 35.0% | |
| TTAAGAAACGCTGTGTAAAG+AGG | - | chr4_2:21241491-21241510 | Msa0594150:intron | 35.0% | |
| CTGATCTAGTGCTTCTTTAT+TGG | - | chr4_2:21241772-21241791 | Msa0594150:intron | 35.0% | |
| AGTCTGGTATAAGAAGGATA+GGG | + | chr4_2:21242030-21242049 | None:intergenic | 35.0% | |
| CAATACAGTCTGGTATAAGA+AGG | + | chr4_2:21242036-21242055 | None:intergenic | 35.0% | |
| CTTCTTATACCAGACTGTAT+TGG | - | chr4_2:21242034-21242053 | Msa0594150:CDS | 35.0% | |
| TAAGACAATCCAATACAGTC+TGG | + | chr4_2:21242046-21242065 | None:intergenic | 35.0% | |
| ATTCAAACCAGAAATAGCAC+CGG | + | chr4_2:21241337-21241356 | None:intergenic | 35.0% | |
| TTAAGAAACGCTGTGTAAAG+AGG | - | chr4_2:21241491-21241510 | Msa0594150:intron | 35.0% | |
| CTGATCTAGTGCTTCTTTAT+TGG | - | chr4_2:21241772-21241791 | Msa0594150:intron | 35.0% | |
| AGTCTGGTATAAGAAGGATA+GGG | + | chr4_2:21242030-21242049 | None:intergenic | 35.0% | |
| CAATACAGTCTGGTATAAGA+AGG | + | chr4_2:21242036-21242055 | None:intergenic | 35.0% | |
| CTTCTTATACCAGACTGTAT+TGG | - | chr4_2:21242034-21242053 | Msa0594150:CDS | 35.0% | |
| TAAGACAATCCAATACAGTC+TGG | + | chr4_2:21242046-21242065 | None:intergenic | 35.0% | |
| ATCTCATGTGGACAAGTAAC+TGG | - | chr4_2:21241171-21241190 | Msa0594150:three_prime_UTR | 40.0% | |
| !! | CCTCTAAGATATGCAATGCA+TGG | + | chr4_2:21241207-21241226 | None:intergenic | 40.0% |
| CCATGCATTGCATATCTTAG+AGG | - | chr4_2:21241204-21241223 | Msa0594150:three_prime_UTR | 40.0% | |
| TGCATTGCATATCTTAGAGG+TGG | - | chr4_2:21241207-21241226 | Msa0594150:three_prime_UTR | 40.0% | |
| TGTTAACACCACATTTGCCA+GGG | + | chr4_2:21241380-21241399 | None:intergenic | 40.0% | |
| ATGTTAACACCACATTTGCC+AGG | + | chr4_2:21241381-21241400 | None:intergenic | 40.0% | |
| !! | GTAGAGGTACTGATCTTGTA+GGG | + | chr4_2:21241405-21241424 | None:intergenic | 40.0% |
| AAGAACTTACGTAGCACAGT+TGG | + | chr4_2:21241427-21241446 | None:intergenic | 40.0% | |
| TATTCAAGTCGGTCGAAAAG+TGG | - | chr4_2:21241826-21241845 | Msa0594150:intron | 40.0% | |
| CAGTCTGGTATAAGAAGGAT+AGG | + | chr4_2:21242031-21242050 | None:intergenic | 40.0% | |
| ATCTCATGTGGACAAGTAAC+TGG | - | chr4_2:21241171-21241190 | Msa0594150:three_prime_UTR | 40.0% | |
| !! | CCTCTAAGATATGCAATGCA+TGG | + | chr4_2:21241207-21241226 | None:intergenic | 40.0% |
| CCATGCATTGCATATCTTAG+AGG | - | chr4_2:21241204-21241223 | Msa0594150:three_prime_UTR | 40.0% | |
| TGCATTGCATATCTTAGAGG+TGG | - | chr4_2:21241207-21241226 | Msa0594150:three_prime_UTR | 40.0% | |
| TGTTAACACCACATTTGCCA+GGG | + | chr4_2:21241380-21241399 | None:intergenic | 40.0% | |
| ATGTTAACACCACATTTGCC+AGG | + | chr4_2:21241381-21241400 | None:intergenic | 40.0% | |
| !! | GTAGAGGTACTGATCTTGTA+GGG | + | chr4_2:21241405-21241424 | None:intergenic | 40.0% |
| AAGAACTTACGTAGCACAGT+TGG | + | chr4_2:21241427-21241446 | None:intergenic | 40.0% | |
| TATTCAAGTCGGTCGAAAAG+TGG | - | chr4_2:21241826-21241845 | Msa0594150:intron | 40.0% | |
| CAGTCTGGTATAAGAAGGAT+AGG | + | chr4_2:21242031-21242050 | None:intergenic | 40.0% | |
| GTGCATGATTGTTGCACCTA+TGG | - | chr4_2:21241137-21241156 | Msa0594150:three_prime_UTR | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+CGG | - | chr4_2:21241315-21241334 | Msa0594150:three_prime_UTR | 45.0% |
| TTGAATGCTAACACAGCTGC+TGG | - | chr4_2:21241351-21241370 | Msa0594150:three_prime_UTR | 45.0% | |
| !! | GGTAGAGGTACTGATCTTGT+AGG | + | chr4_2:21241406-21241425 | None:intergenic | 45.0% |
| !! | TTACGTAGCACAGTTGGTAG+AGG | + | chr4_2:21241421-21241440 | None:intergenic | 45.0% |
| AACGCTGTGTAAAGAGGTCT+AGG | - | chr4_2:21241497-21241516 | Msa0594150:intron | 45.0% | |
| GGCACTCTCATATACTAGTG+AGG | + | chr4_2:21241952-21241971 | None:intergenic | 45.0% | |
| ATGGAGGCTCATCTTATGTG+TGG | - | chr4_2:21242004-21242023 | Msa0594150:CDS | 45.0% | |
| GTGCATGATTGTTGCACCTA+TGG | - | chr4_2:21241137-21241156 | Msa0594150:three_prime_UTR | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+CGG | - | chr4_2:21241315-21241334 | Msa0594150:three_prime_UTR | 45.0% |
| TTGAATGCTAACACAGCTGC+TGG | - | chr4_2:21241351-21241370 | Msa0594150:three_prime_UTR | 45.0% | |
| !! | GGTAGAGGTACTGATCTTGT+AGG | + | chr4_2:21241406-21241425 | None:intergenic | 45.0% |
| !! | TTACGTAGCACAGTTGGTAG+AGG | + | chr4_2:21241421-21241440 | None:intergenic | 45.0% |
| AACGCTGTGTAAAGAGGTCT+AGG | - | chr4_2:21241497-21241516 | Msa0594150:intron | 45.0% | |
| GGCACTCTCATATACTAGTG+AGG | + | chr4_2:21241952-21241971 | None:intergenic | 45.0% | |
| ATGGAGGCTCATCTTATGTG+TGG | - | chr4_2:21242004-21242023 | Msa0594150:CDS | 45.0% | |
| !!! | AAAAATTATAATATTTTTAG+AGG | - | chr4_2:21241657-21241676 | Msa0594150:intron | 5.0% |
| !!! | AAAAATTATAATATTTTTAG+AGG | - | chr4_2:21241657-21241676 | Msa0594150:intron | 5.0% |
| GAGATTGCAGCATCTGCCAT+AGG | + | chr4_2:21241156-21241175 | None:intergenic | 50.0% | |
| GCAGATGCTGCAATCTCATG+TGG | - | chr4_2:21241159-21241178 | Msa0594150:three_prime_UTR | 50.0% | |
| GCATATCTTAGAGGTGGTGC+TGG | - | chr4_2:21241213-21241232 | Msa0594150:three_prime_UTR | 50.0% | |
| GAGATTGCAGCATCTGCCAT+AGG | + | chr4_2:21241156-21241175 | None:intergenic | 50.0% | |
| GCAGATGCTGCAATCTCATG+TGG | - | chr4_2:21241159-21241178 | Msa0594150:three_prime_UTR | 50.0% | |
| GCATATCTTAGAGGTGGTGC+TGG | - | chr4_2:21241213-21241232 | Msa0594150:three_prime_UTR | 50.0% | |
| TTCACTCCAGCACAGCATGC+TGG | + | chr4_2:21241246-21241265 | None:intergenic | 55.0% | |
| AGCAGTTGCAAGCAGCCTGA+CGG | + | chr4_2:21241302-21241321 | None:intergenic | 55.0% | |
| !! | AGTGCTGCCGGTGCTATTTC+TGG | - | chr4_2:21241327-21241346 | Msa0594150:three_prime_UTR | 55.0% |
| TTCACTCCAGCACAGCATGC+TGG | + | chr4_2:21241246-21241265 | None:intergenic | 55.0% | |
| AGCAGTTGCAAGCAGCCTGA+CGG | + | chr4_2:21241302-21241321 | None:intergenic | 55.0% | |
| !! | AGTGCTGCCGGTGCTATTTC+TGG | - | chr4_2:21241327-21241346 | Msa0594150:three_prime_UTR | 55.0% |
| CCAGCACAGCATGCTGGAGA+TGG | + | chr4_2:21241240-21241259 | None:intergenic | 60.0% | |
| CCATCTCCAGCATGCTGTGC+TGG | - | chr4_2:21241237-21241256 | Msa0594150:three_prime_UTR | 60.0% | |
| TTGCAAGCAGCCTGACGGTC+AGG | + | chr4_2:21241297-21241316 | None:intergenic | 60.0% | |
| ! | AACACAGCTGCTGGTCTCCC+TGG | - | chr4_2:21241360-21241379 | Msa0594150:three_prime_UTR | 60.0% |
| ! | GCTGGTCTCCCTGGCAAATG+TGG | - | chr4_2:21241369-21241388 | Msa0594150:three_prime_UTR | 60.0% |
| CCAGCACAGCATGCTGGAGA+TGG | + | chr4_2:21241240-21241259 | None:intergenic | 60.0% | |
| CCATCTCCAGCATGCTGTGC+TGG | - | chr4_2:21241237-21241256 | Msa0594150:three_prime_UTR | 60.0% | |
| TTGCAAGCAGCCTGACGGTC+AGG | + | chr4_2:21241297-21241316 | None:intergenic | 60.0% | |
| ! | AACACAGCTGCTGGTCTCCC+TGG | - | chr4_2:21241360-21241379 | Msa0594150:three_prime_UTR | 60.0% |
| ! | GCTGGTCTCCCTGGCAAATG+TGG | - | chr4_2:21241369-21241388 | Msa0594150:three_prime_UTR | 60.0% |
| !! | ACGGTCAGGCGTGGTGGTGG+CGG | + | chr4_2:21241283-21241302 | None:intergenic | 70.0% |
| CTGACGGTCAGGCGTGGTGG+TGG | + | chr4_2:21241286-21241305 | None:intergenic | 70.0% | |
| CACCACCACGCCTGACCGTC+AGG | - | chr4_2:21241284-21241303 | Msa0594150:three_prime_UTR | 70.0% | |
| AGCCTGACGGTCAGGCGTGG+TGG | + | chr4_2:21241289-21241308 | None:intergenic | 70.0% | |
| AGCAGCCTGACGGTCAGGCG+TGG | + | chr4_2:21241292-21241311 | None:intergenic | 70.0% | |
| !! | ACGGTCAGGCGTGGTGGTGG+CGG | + | chr4_2:21241283-21241302 | None:intergenic | 70.0% |
| CTGACGGTCAGGCGTGGTGG+TGG | + | chr4_2:21241286-21241305 | None:intergenic | 70.0% | |
| CACCACCACGCCTGACCGTC+AGG | - | chr4_2:21241284-21241303 | Msa0594150:three_prime_UTR | 70.0% | |
| AGCCTGACGGTCAGGCGTGG+TGG | + | chr4_2:21241289-21241308 | None:intergenic | 70.0% | |
| AGCAGCCTGACGGTCAGGCG+TGG | + | chr4_2:21241292-21241311 | None:intergenic | 70.0% | |
| !! | GTCAGGCGTGGTGGTGGCGG+CGG | + | chr4_2:21241280-21241299 | None:intergenic | 75.0% |
| !! | GTCAGGCGTGGTGGTGGCGG+CGG | + | chr4_2:21241280-21241299 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 21241099 | 21242302 | 21241099 | ID=Msa0594150;Name=Msa0594150 |
| chr4_2 | mRNA | 21241099 | 21242302 | 21241099 | ID=Msa0594150-mRNA-1;Parent=Msa0594150;Name=Msa0594150-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0.5|1|1|1|2|325|130 |
| chr4_2 | exon | 21241099 | 21241500 | 21241099 | ID=Msa0594150-mRNA-1:exon:5946;Parent=Msa0594150-mRNA-1 |
| chr4_2 | exon | 21241987 | 21242302 | 21241987 | ID=Msa0594150-mRNA-1:exon:5945;Parent=Msa0594150-mRNA-1 |
| chr4_2 | CDS | 21241987 | 21242302 | 21241987 | ID=Msa0594150-mRNA-1:cds;Parent=Msa0594150-mRNA-1 |
| chr4_2 | CDS | 21241424 | 21241500 | 21241424 | ID=Msa0594150-mRNA-1:cds;Parent=Msa0594150-mRNA-1 |
| chr4_2 | three_prime_UTR | 21241099 | 21241423 | 21241099 | ID=Msa0594150-mRNA-1:three_prime_utr;Parent=Msa0594150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
