AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1374240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1374240 MtrunA17_Chr8g0392251 99.286 140 1 0 1 140 1 140 2.40e-100 283
Msa1374240 MtrunA17_Chr8g0392191 99.286 140 1 0 1 140 1 140 2.40e-100 283
Msa1374240 MtrunA17_Chr5g0432551 98.571 140 2 0 1 140 1 140 6.11e-100 282
Msa1374240 MtrunA17_Chr4g0044571 94.286 140 8 0 1 140 4 143 7.79e-96 272
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1374240 AT2G09990.1 89.209 139 15 0 2 140 8 146 1.92e-91 261
Msa1374240 AT5G18380.1 88.489 139 16 0 2 140 8 146 3.25e-91 261
Msa1374240 AT3G04230.1 83.453 139 23 0 2 140 8 146 6.19e-85 245
Msa1374240 AT5G18380.2 86.400 125 17 0 2 126 8 132 1.67e-78 229
Msa1374240 AT5G18380.3 86.111 108 15 0 2 109 8 115 5.55e-67 199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTAACGATAAGATTTC+TGG 0.210396 tig0019471:-83575 None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG 0.239341 tig0019471:+83458 Msa1374240:CDS
CCGCAACGGCGTTCTTCTTC+CGG 0.308388 tig0019471:-83194 None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG 0.344740 tig0019471:+83281 Msa1374240:CDS
CGCTGTGAGCCTAAGAAGTT+TGG 0.359075 tig0019471:+83528 Msa1374240:CDS
TCAGAGTATTGCTAAGGCTT+TGG 0.386737 tig0019471:+83407 Msa1374240:CDS
CCACGACCACCAAACTTCTT+AGG 0.436009 tig0019471:-83537 None:intergenic
TACGAACCAATCCTCCTTCT+CGG 0.444217 tig0019471:+83306 Msa1374240:CDS
GTTTCGCCGGCGTTGATATG+AGG 0.478501 tig0019471:+83337 Msa1374240:CDS
GCCTTGAAACGAAGAATCTC+AGG 0.478888 tig0019471:-83282 None:intergenic
CTCGGAAGACACCGTTTCGC+CGG 0.484088 tig0019471:+83324 Msa1374240:CDS
ATGGAACAAGTGCAATGCTT+TGG 0.487256 tig0019471:+83171 Msa1374240:CDS
CAACGGCTGTCCAATCGAAC+TGG 0.488470 tig0019471:+83254 Msa1374240:CDS
GTGGTCGTGGTGCTCGTGCT+AGG 0.502073 tig0019471:+83550 Msa1374240:CDS
CCTAAGAAGTTTGGTGGTCG+TGG 0.511400 tig0019471:+83537 Msa1374240:CDS
AGGATCCGTGTGAAGGGAGG+AGG 0.521481 tig0019471:+83357 Msa1374240:CDS
TCATATCAACGCCGGCGAAA+CGG 0.524265 tig0019471:-83335 None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG 0.524396 tig0019471:-83264 None:intergenic
AACAAGTGCAATGCTTTGGC+CGG 0.546867 tig0019471:+83175 Msa1374240:CDS
AACGGTGTCTTCCGAGAAGG+AGG 0.549270 tig0019471:-83317 None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG 0.558448 tig0019471:+83531 Msa1374240:CDS
GACCGCGACCTCTCTTGCAG+TGG 0.558828 tig0019471:-83221 None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG 0.562945 tig0019471:+83219 Msa1374240:CDS
CATTCGTCAGAGTATTGCTA+AGG 0.576627 tig0019471:+83401 Msa1374240:CDS
TGATATGAGGATCCGTGTGA+AGG 0.579448 tig0019471:+83350 Msa1374240:CDS
GCGGTAACCCACTGCAAGAG+AGG 0.580471 tig0019471:+83213 Msa1374240:CDS
TTCTTAGGCTCACAGCGTCT+AGG 0.590494 tig0019471:-83522 None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG 0.592227 tig0019471:-83312 None:intergenic
GATATGAGGATCCGTGTGAA+GGG 0.594162 tig0019471:+83351 Msa1374240:CDS
CTTGCAGTGGGTTACCGCAA+CGG 0.599991 tig0019471:-83208 None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG 0.603590 tig0019471:-83220 None:intergenic
CGCGGTCTGATCAAGATCAA+CGG 0.609302 tig0019471:+83237 Msa1374240:CDS
CTTAGCAATACTCTGACGAA+TGG 0.614188 tig0019471:-83400 None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG 0.615322 tig0019471:+83354 Msa1374240:CDS
CGAAACGGTGTCTTCCGAGA+AGG 0.622095 tig0019471:-83320 None:intergenic
TGTGACCTCCTCCCTTCACA+CGG 0.629480 tig0019471:-83362 None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG 0.651449 tig0019471:+83194 Msa1374240:CDS
ACGGATCCTCATATCAACGC+CGG 0.685813 tig0019471:-83343 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAGTAAGAAAGAGATTA+AGG + tig0019471:83458-83477 Msa1374240:CDS 30.0%
ATGGAACAAGTGCAATGCTT+TGG + tig0019471:83171-83190 Msa1374240:CDS 40.0%
CTTAGCAATACTCTGACGAA+TGG - tig0019471:83403-83422 None:intergenic 40.0%
!! CATTCGTCAGAGTATTGCTA+AGG + tig0019471:83401-83420 Msa1374240:CDS 40.0%
!! TCAGAGTATTGCTAAGGCTT+TGG + tig0019471:83407-83426 Msa1374240:CDS 40.0%
AACAAGTGCAATGCTTTGGC+CGG + tig0019471:83175-83194 Msa1374240:CDS 45.0%
GCCTGAGATTCTTCGTTTCA+AGG + tig0019471:83281-83300 Msa1374240:CDS 45.0%
GCCTTGAAACGAAGAATCTC+AGG - tig0019471:83285-83304 None:intergenic 45.0%
TACGAACCAATCCTCCTTCT+CGG + tig0019471:83306-83325 Msa1374240:CDS 45.0%
TGATATGAGGATCCGTGTGA+AGG + tig0019471:83350-83369 Msa1374240:CDS 45.0%
GATATGAGGATCCGTGTGAA+GGG + tig0019471:83351-83370 Msa1374240:CDS 45.0%
TGTGAGCCTAAGAAGTTTGG+TGG + tig0019471:83531-83550 Msa1374240:CDS 45.0%
CGCGGTCTGATCAAGATCAA+CGG + tig0019471:83237-83256 Msa1374240:CDS 50.0%
TCAGGCTCAACCAGTTCGAT+TGG - tig0019471:83267-83286 None:intergenic 50.0%
TGTCTTCCGAGAAGGAGGAT+TGG - tig0019471:83315-83334 None:intergenic 50.0%
TCATATCAACGCCGGCGAAA+CGG - tig0019471:83338-83357 None:intergenic 50.0%
ACGGATCCTCATATCAACGC+CGG - tig0019471:83346-83365 None:intergenic 50.0%
TTCTTAGGCTCACAGCGTCT+AGG - tig0019471:83525-83544 None:intergenic 50.0%
CGCTGTGAGCCTAAGAAGTT+TGG + tig0019471:83528-83547 Msa1374240:CDS 50.0%
CCACGACCACCAAACTTCTT+AGG - tig0019471:83540-83559 None:intergenic 50.0%
! CCTAAGAAGTTTGGTGGTCG+TGG + tig0019471:83537-83556 Msa1374240:CDS 50.0%
!! CTTGCAGTGGGTTACCGCAA+CGG - tig0019471:83211-83230 None:intergenic 55.0%
CAACGGCTGTCCAATCGAAC+TGG + tig0019471:83254-83273 Msa1374240:CDS 55.0%
AACGGTGTCTTCCGAGAAGG+AGG - tig0019471:83320-83339 None:intergenic 55.0%
CGAAACGGTGTCTTCCGAGA+AGG - tig0019471:83323-83342 None:intergenic 55.0%
!! GTTTCGCCGGCGTTGATATG+AGG + tig0019471:83337-83356 Msa1374240:CDS 55.0%
ATGAGGATCCGTGTGAAGGG+AGG + tig0019471:83354-83373 Msa1374240:CDS 55.0%
TGTGACCTCCTCCCTTCACA+CGG - tig0019471:83365-83384 None:intergenic 55.0%
CCGCAACGGCGTTCTTCTTC+CGG - tig0019471:83197-83216 None:intergenic 60.0%
CCGGAAGAAGAACGCCGTTG+CGG + tig0019471:83194-83213 Msa1374240:CDS 60.0%
GCGGTAACCCACTGCAAGAG+AGG + tig0019471:83213-83232 Msa1374240:CDS 60.0%
ACCCACTGCAAGAGAGGTCG+CGG + tig0019471:83219-83238 Msa1374240:CDS 60.0%
ACCGCGACCTCTCTTGCAGT+GGG - tig0019471:83223-83242 None:intergenic 60.0%
CTCGGAAGACACCGTTTCGC+CGG + tig0019471:83324-83343 Msa1374240:CDS 60.0%
AGGATCCGTGTGAAGGGAGG+AGG + tig0019471:83357-83376 Msa1374240:CDS 60.0%
GACCGCGACCTCTCTTGCAG+TGG - tig0019471:83224-83243 None:intergenic 65.0%
GTGGTCGTGGTGCTCGTGCT+AGG + tig0019471:83550-83569 Msa1374240:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0019471 gene 83171 83593 83171 ID=Msa1374240;Name=Msa1374240
tig0019471 mRNA 83171 83593 83171 ID=Msa1374240-mRNA-1;Parent=Msa1374240;Name=Msa1374240-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|140
tig0019471 exon 83171 83593 83171 ID=Msa1374240-mRNA-1:exon:3765;Parent=Msa1374240-mRNA-1
tig0019471 CDS 83171 83593 83171 ID=Msa1374240-mRNA-1:cds;Parent=Msa1374240-mRNA-1
Gene Sequence

>Msa1374240

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>Msa1374240

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*