AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0683840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683840 MtrunA17_Chr4g0030911 88.797 241 0 1 1 241 1 214 1.13e-153 426
Msa0683840 MtrunA17_Chr1g0182321 81.405 242 17 2 1 241 1 215 5.50e-142 396
Msa0683840 MtrunA17_Chr1g0182301 80.992 242 18 2 1 241 1 215 3.15e-141 394
Msa0683840 MtrunA17_Chr1g0212521 83.190 232 11 2 11 241 11 215 3.42e-135 379
Msa0683840 MtrunA17_Chr7g0263071 84.052 232 9 2 11 241 11 215 6.98e-132 370
Msa0683840 MtrunA17_Chr2g0327911 66.942 242 50 3 1 241 1 213 1.06e-109 314
Msa0683840 MtrunA17_Chr7g0250531 54.936 233 68 5 10 240 3 200 1.64e-81 242
Msa0683840 MtrunA17_Chr4g0055651 53.017 232 72 5 11 240 4 200 5.10e-80 238
Msa0683840 MtrunA17_Chr8g0335471 53.648 233 70 5 11 240 4 201 1.92e-78 234
Msa0683840 MtrunA17_Chr2g0276931 49.138 232 84 4 11 241 5 203 2.21e-73 222
Msa0683840 MtrunA17_Chr6g0458081 42.373 236 101 5 12 240 3 210 3.46e-54 173
Msa0683840 MtrunA17_Chr2g0290891 41.525 236 103 4 12 240 3 210 6.63e-54 172
Msa0683840 MtrunA17_Chr3g0112681 40.417 240 100 4 11 240 8 214 1.72e-53 171
Msa0683840 MtrunA17_Chr4g0032201 51.316 152 73 1 7 158 4 154 2.12e-53 171
Msa0683840 MtrunA17_Chr2g0283131 41.079 241 105 5 7 240 5 215 1.38e-51 167
Msa0683840 MtrunA17_Chr2g0276941 51.351 148 71 1 11 158 11 157 1.76e-51 166
Msa0683840 MtrunA17_Chr2g0315551 53.289 152 70 1 7 158 5 155 2.35e-51 166
Msa0683840 MtrunA17_Chr1g0189361 52.055 146 69 1 13 158 12 156 1.42e-50 164
Msa0683840 MtrunA17_Chr4g0046261 39.834 241 108 5 7 240 5 215 2.81e-50 163
Msa0683840 MtrunA17_Chr4g0040781 39.662 237 114 4 7 240 5 215 3.60e-50 163
Msa0683840 MtrunA17_Chr3g0123821 37.344 241 115 4 8 240 9 221 5.65e-50 163
Msa0683840 MtrunA17_Chr2g0319951 51.316 152 73 1 7 158 5 155 9.51e-50 162
Msa0683840 MtrunA17_Chr1g0180291 42.424 231 100 6 12 240 11 210 4.01e-49 160
Msa0683840 MtrunA17_Chr8g0381841 50.000 146 72 1 13 158 15 159 1.08e-48 159
Msa0683840 MtrunA17_Chr8g0349711 38.819 237 105 5 14 240 5 211 1.29e-48 159
Msa0683840 MtrunA17_Chr7g0265001 41.126 231 104 5 12 240 13 213 6.27e-48 157
Msa0683840 MtrunA17_Chr7g0234201 38.158 228 115 4 14 240 12 214 4.06e-47 155
Msa0683840 MtrunA17_Chr8g0349681 38.034 234 115 3 9 240 378 583 1.61e-46 162
Msa0683840 MtrunA17_Chr8g0349681 38.739 222 102 4 12 229 172 363 3.36e-42 150
Msa0683840 MtrunA17_Chr8g0349681 48.276 145 74 1 14 158 12 155 1.73e-40 146
Msa0683840 MtrunA17_Chr3g0130271 51.007 149 70 3 11 157 8 155 2.58e-46 153
Msa0683840 MtrunA17_Chr1g0159481 41.026 234 101 9 11 240 13 213 5.48e-46 152
Msa0683840 MtrunA17_Chr8g0384311 49.351 154 74 4 6 157 4 155 6.46e-46 152
Msa0683840 MtrunA17_Chr2g0325091 38.655 238 106 5 11 240 7 212 8.45e-46 152
Msa0683840 MtrunA17_Chr8g0351861 50.000 146 72 1 13 158 14 158 1.32e-45 152
Msa0683840 MtrunA17_Chr1g0197331 37.449 243 117 6 1 240 1 211 3.51e-45 150
Msa0683840 MtrunA17_Chr1g0174281 36.626 243 108 5 13 241 13 223 5.42e-45 150
Msa0683840 MtrunA17_Chr8g0346421 47.260 146 76 1 13 158 24 168 6.98e-45 150
Msa0683840 MtrunA17_Chr7g0214241 35.193 233 118 5 14 240 14 219 9.98e-44 147
Msa0683840 MtrunA17_Chr7g0275991 44.079 152 84 1 7 158 12 162 1.24e-43 147
Msa0683840 MtrunA17_Chr6g0482721 41.146 192 85 2 13 204 10 173 2.24e-42 143
Msa0683840 MtrunA17_Chr7g0231131 45.890 146 78 1 13 158 13 157 3.69e-42 143
Msa0683840 MtrunA17_Chr8g0392931 38.655 238 104 5 13 240 12 217 1.87e-39 135
Msa0683840 MtrunA17_Chr8g0390561 35.193 233 113 5 8 240 2 196 5.16e-39 134
Msa0683840 MtrunA17_Chr1g0199331 38.021 192 90 3 16 206 32 195 1.91e-38 132
Msa0683840 MtrunA17_Chr3g0109721 33.482 224 113 4 17 240 12 199 1.40e-37 130
Msa0683840 MtrunA17_Chr5g0433561 32.589 224 115 4 17 240 12 199 2.08e-36 127
Msa0683840 MtrunA17_Chr8g0382221 42.958 142 80 1 17 158 11 151 8.64e-36 126
Msa0683840 MtrunA17_Chr5g0400131 32.627 236 126 4 5 240 3 205 5.82e-35 124
Msa0683840 MtrunA17_Chr4g0034871 34.615 234 112 8 5 229 2 203 2.92e-34 122
Msa0683840 MtrunA17_Chr3g0123971 42.254 142 81 1 17 158 11 151 6.80e-34 121
Msa0683840 MtrunA17_Chr4g0055081 31.915 235 127 4 5 239 3 204 1.39e-33 120
Msa0683840 MtrunA17_Chr4g0004661 32.759 232 116 6 15 240 64 261 2.19e-30 113
Msa0683840 MtrunA17_Chr2g0331691 32.328 232 117 6 15 240 10 207 2.44e-30 112
Msa0683840 MtrunA17_Chr6g0466301 34.536 194 94 4 15 202 8 174 5.04e-30 111
Msa0683840 MtrunA17_Chr6g0465491 34.536 194 94 4 15 202 8 174 5.04e-30 111
Msa0683840 MtrunA17_Chr8g0383261 32.468 231 115 5 15 239 12 207 9.14e-30 110
Msa0683840 MtrunA17_Chr1g0201121 30.942 223 119 4 15 229 8 203 2.50e-28 106
Msa0683840 MtrunA17_Chr4g0037021 31.606 193 112 5 15 206 6 179 1.52e-22 91.3
Msa0683840 MtrunA17_Chr4g0046211 35.484 124 77 3 15 137 6 127 1.56e-22 89.7
Msa0683840 MtrunA17_Chr2g0325101 31.522 184 106 5 15 197 6 170 4.91e-22 90.1
Msa0683840 MtrunA17_Chr3g0117931 30.539 167 96 5 15 163 8 172 1.41e-21 89.0
Msa0683840 MtrunA17_Chr8g0371501 33.094 139 83 4 15 145 6 142 3.30e-21 87.8
Msa0683840 MtrunA17_Chr5g0405791 33.094 139 83 4 15 145 6 142 5.01e-21 87.4
Msa0683840 MtrunA17_Chr1g0178291 39.456 147 79 4 18 162 431 569 6.10e-21 91.3
Msa0683840 MtrunA17_Chr6g0486041 29.891 184 109 5 15 197 6 170 4.62e-20 85.1
Msa0683840 MtrunA17_Chr6g0466161 30.729 192 100 4 32 217 7 171 6.87e-20 84.7
Msa0683840 MtrunA17_Chr6g0465471 31.638 177 88 4 32 202 7 156 2.04e-19 83.2
Msa0683840 MtrunA17_Chr3g0138121 26.000 200 116 5 5 204 4 171 2.49e-18 80.9
Msa0683840 MtrunA17_Chr3g0138101 26.000 200 116 5 5 204 4 171 2.81e-18 80.5
Msa0683840 MtrunA17_Chr3g0138111 26.000 200 116 5 5 204 4 171 2.99e-18 80.5
Msa0683840 MtrunA17_Chr6g0466431 35.789 95 52 1 15 109 8 93 1.28e-16 73.2
Msa0683840 MtrunA17_Chr6g0465611 35.789 95 52 1 15 109 8 93 1.28e-16 73.2
Msa0683840 MtrunA17_Chr5g0434561 30.055 183 98 7 16 197 20 173 8.14e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683840 AT5G59840.1 83.190 232 11 2 11 241 11 215 3.02e-136 382
Msa0683840 AT3G46060.2 82.759 232 12 2 11 241 11 215 9.45e-136 380
Msa0683840 AT3G46060.3 82.759 232 12 2 11 241 11 215 9.45e-136 380
Msa0683840 AT3G46060.1 82.759 232 12 2 11 241 11 215 9.45e-136 380
Msa0683840 AT3G53610.2 81.897 232 14 2 11 241 11 215 9.44e-134 375
Msa0683840 AT3G53610.3 81.897 232 14 2 11 241 11 215 9.44e-134 375
Msa0683840 AT3G53610.1 81.897 232 14 2 11 241 11 215 9.44e-134 375
Msa0683840 AT5G03520.1 76.667 240 29 1 1 240 1 213 1.02e-123 350
Msa0683840 AT3G09900.1 75.207 242 31 2 1 240 1 215 3.67e-120 341
Msa0683840 AT5G03520.2 73.298 191 24 1 50 240 40 203 1.35e-96 281
Msa0683840 AT1G02130.1 54.701 234 68 5 10 240 3 201 3.44e-83 247
Msa0683840 AT5G47200.1 52.586 232 73 5 11 240 4 200 2.25e-76 229
Msa0683840 AT4G17530.1 52.155 232 74 5 11 240 4 200 4.78e-76 229
Msa0683840 AT3G11730.1 50.424 236 77 5 11 241 4 204 3.80e-74 224
Msa0683840 AT4G35860.1 43.460 237 99 5 12 241 3 211 2.31e-58 184
Msa0683840 AT4G17160.1 41.810 232 104 4 12 241 3 205 4.16e-56 178
Msa0683840 AT4G17170.1 41.772 237 103 4 12 241 3 211 6.71e-56 178
Msa0683840 AT1G09630.1 40.586 239 108 4 7 239 4 214 1.30e-52 170
Msa0683840 AT1G16920.1 52.632 152 71 1 7 158 5 155 1.39e-52 169
Msa0683840 AT5G59150.1 50.658 152 74 1 7 158 4 154 2.75e-52 169
Msa0683840 AT3G46830.1 50.658 152 74 1 7 158 4 154 1.73e-51 167
Msa0683840 AT3G46830.2 50.658 152 74 1 7 158 4 154 1.73e-51 167
Msa0683840 AT4G18430.1 39.331 239 112 4 7 240 5 215 4.95e-51 166
Msa0683840 AT1G07410.1 49.342 152 76 1 7 158 4 154 7.45e-51 165
Msa0683840 AT4G39990.1 39.149 235 108 4 13 240 15 221 1.27e-49 162
Msa0683840 AT3G15060.1 51.974 152 72 1 7 158 5 155 1.57e-49 162
Msa0683840 AT5G60860.1 39.834 241 108 5 7 240 5 215 1.60e-49 162
Msa0683840 AT1G28550.1 51.316 152 73 1 7 158 5 155 6.04e-49 160
Msa0683840 AT5G45750.1 50.000 152 75 1 7 158 5 155 6.70e-49 160
Msa0683840 AT3G07410.1 39.056 233 110 5 13 240 10 215 8.65e-49 160
Msa0683840 AT4G18800.1 50.658 152 74 1 7 158 5 155 9.63e-49 160
Msa0683840 AT1G06400.1 50.658 152 74 1 7 158 5 155 1.47e-48 159
Msa0683840 AT5G47520.1 51.370 146 70 1 13 158 12 156 4.92e-48 158
Msa0683840 AT2G33870.1 39.918 243 108 6 7 241 5 217 6.38e-48 158
Msa0683840 AT1G05810.1 48.630 146 74 1 13 158 10 154 1.72e-47 157
Msa0683840 AT2G43130.1 48.630 146 74 1 13 158 10 154 2.44e-47 156
Msa0683840 AT1G05810.2 48.630 146 74 1 13 158 56 200 2.93e-47 157
Msa0683840 AT5G47960.1 50.000 146 72 1 13 158 13 157 6.13e-47 155
Msa0683840 AT1G01200.1 42.857 196 85 3 13 208 26 194 6.68e-47 155
Msa0683840 AT2G31680.1 47.945 146 75 1 13 158 10 154 1.26e-46 154
Msa0683840 AT5G65270.1 47.260 146 76 1 13 158 15 159 5.09e-46 153
Msa0683840 AT1G43890.3 43.814 194 78 5 6 197 4 168 2.51e-45 151
Msa0683840 AT1G43890.1 43.814 194 78 5 6 197 4 168 2.51e-45 151
Msa0683840 AT1G43890.2 43.814 194 78 5 6 197 4 168 2.51e-45 151
Msa0683840 AT3G12160.1 36.441 236 115 4 13 241 13 220 2.72e-45 151
Msa0683840 AT1G73640.1 40.930 215 95 3 13 223 11 197 9.70e-45 150
Msa0683840 AT1G18200.1 43.367 196 83 2 13 208 11 178 1.28e-44 149
Msa0683840 AT5G03530.2 39.241 237 111 6 6 240 4 209 4.33e-44 147
Msa0683840 AT5G03530.1 39.241 237 111 6 6 240 4 209 4.33e-44 147
Msa0683840 AT3G54840.2 45.860 157 80 3 3 158 24 176 4.01e-42 142
Msa0683840 AT3G54840.1 45.860 157 80 3 3 158 24 176 5.66e-42 142
Msa0683840 AT3G54840.3 47.183 142 73 2 18 158 2 142 6.11e-41 138
Msa0683840 AT4G35860.2 40.625 192 79 5 57 241 2 165 1.57e-38 132
Msa0683840 AT3G09910.5 39.726 219 101 6 8 224 6 195 5.63e-38 131
Msa0683840 AT3G09910.4 39.726 219 101 6 8 224 6 195 5.63e-38 131
Msa0683840 AT3G09910.3 45.251 179 84 6 8 174 6 182 3.49e-37 130
Msa0683840 AT3G09910.1 45.251 179 84 6 8 174 6 182 3.49e-37 130
Msa0683840 AT3G09910.2 45.251 179 84 6 8 174 6 182 3.49e-37 130
Msa0683840 AT4G19640.1 34.667 225 111 6 17 241 12 200 4.34e-37 129
Msa0683840 AT5G45130.1 32.444 225 116 4 17 241 12 200 9.51e-37 128
Msa0683840 AT2G22290.1 33.036 224 121 3 17 240 11 205 3.87e-36 127
Msa0683840 AT3G18820.1 35.043 234 111 7 5 229 2 203 2.54e-35 125
Msa0683840 AT5G45130.2 31.839 223 116 4 19 241 22 208 3.98e-35 124
Msa0683840 AT4G39890.1 37.097 186 88 3 17 201 11 168 7.02e-35 124
Msa0683840 AT5G10260.1 32.444 225 123 3 17 241 11 206 7.62e-35 124
Msa0683840 AT5G59150.2 50.980 102 49 1 57 158 3 103 7.53e-33 117
Msa0683840 AT1G52280.1 42.000 150 81 3 15 158 8 157 2.29e-32 117
Msa0683840 AT2G44610.1 39.610 154 88 2 5 158 3 151 3.99e-32 117
Msa0683840 AT5G64990.1 41.549 142 82 1 17 158 9 149 1.33e-31 115
Msa0683840 AT1G49300.2 38.415 164 91 4 5 162 2 161 2.42e-31 115
Msa0683840 AT1G49300.1 38.415 164 91 4 5 162 2 161 2.42e-31 115
Msa0683840 AT4G09720.1 35.567 194 92 3 15 202 8 174 1.71e-30 112
Msa0683840 AT3G16100.1 40.667 150 83 2 15 158 8 157 1.71e-30 112
Msa0683840 AT1G22740.1 34.123 211 106 4 15 219 8 191 2.96e-30 112
Msa0683840 AT4G19640.2 39.669 121 73 0 17 137 12 132 4.24e-30 110
Msa0683840 AT4G19640.3 39.669 121 73 0 17 137 12 132 2.29e-29 109
Msa0683840 AT2G21880.1 32.812 192 100 4 15 202 9 175 3.89e-29 109
Msa0683840 AT5G64990.2 39.597 149 82 2 17 158 9 156 8.57e-29 108
Msa0683840 AT4G09720.4 35.176 199 91 4 15 202 8 179 1.48e-28 107
Msa0683840 AT5G39620.1 33.163 196 92 4 16 202 6 171 1.17e-27 105
Msa0683840 AT4G09720.3 33.659 205 92 4 15 202 8 185 2.83e-27 104
Msa0683840 AT4G35020.1 32.609 184 104 5 15 197 6 170 2.17e-23 94.0
Msa0683840 AT4G35020.2 32.609 184 104 5 15 197 6 170 2.17e-23 94.0
Msa0683840 AT4G35020.3 32.609 184 104 5 15 197 6 170 2.17e-23 94.0
Msa0683840 AT5G62880.1 30.612 196 116 5 15 209 8 184 3.92e-23 93.6
Msa0683840 AT3G48040.2 30.409 171 102 5 15 170 8 176 6.53e-23 92.0
Msa0683840 AT5G45970.1 31.522 184 106 5 15 197 6 170 2.00e-22 91.7
Msa0683840 AT3G48040.1 31.579 152 92 4 15 156 8 157 4.71e-22 90.9
Msa0683840 AT4G09720.6 35.152 165 74 3 44 202 3 140 1.30e-21 88.6
Msa0683840 AT4G09720.5 35.152 165 74 3 44 202 3 140 1.30e-21 88.6
Msa0683840 AT4G09720.2 35.152 165 74 3 44 202 3 140 1.30e-21 88.6
Msa0683840 AT5G62880.2 33.108 148 88 4 15 161 8 145 2.06e-21 88.6
Msa0683840 AT2G44690.1 31.757 148 89 4 15 152 18 163 2.21e-21 89.0
Msa0683840 AT2G17800.2 31.737 167 94 6 15 163 6 170 2.21e-21 88.6
Msa0683840 AT2G17800.1 31.737 167 94 6 15 163 6 170 2.21e-21 88.6
Msa0683840 AT1G20090.1 33.571 140 83 4 15 146 5 142 2.26e-21 88.6
Msa0683840 AT3G51300.1 31.757 148 89 5 15 152 6 151 3.47e-21 88.2
Msa0683840 AT1G75840.1 32.857 140 84 4 15 146 6 143 3.89e-21 87.8
Msa0683840 AT2G21880.4 31.737 167 85 4 40 202 9 150 3.92e-21 87.8
Msa0683840 AT2G21880.3 31.737 167 85 4 40 202 9 150 3.92e-21 87.8
Msa0683840 AT4G35950.1 31.737 167 94 6 15 163 6 170 4.60e-21 87.8
Msa0683840 AT4G28950.1 30.822 146 91 4 15 152 6 149 1.01e-19 84.7
Msa0683840 AT2G21880.2 28.646 192 100 5 15 202 9 167 1.92e-19 83.6
Msa0683840 AT4G35020.4 37.069 116 70 3 15 129 6 119 2.99e-19 81.6
Msa0683840 AT4G35020.5 37.069 116 70 3 15 129 6 119 2.99e-19 81.6
Msa0683840 AT5G55080.1 28.042 189 105 4 16 204 14 171 1.09e-18 82.0
Msa0683840 AT5G55190.1 26.000 200 116 5 5 204 4 171 3.36e-18 80.9
Msa0683840 AT5G20020.1 26.238 202 117 5 3 204 2 171 3.84e-18 80.5
Msa0683840 AT5G20010.1 26.238 202 117 5 3 204 2 171 4.26e-18 80.5
Msa0683840 AT5G37680.1 27.869 183 102 7 16 197 20 173 3.96e-12 63.5
Msa0683840 AT2G15310.1 28.058 139 88 6 16 153 18 145 2.38e-11 61.6
Msa0683840 AT5G46025.1 33.735 83 53 2 77 158 6 87 4.49e-11 58.9

Find 82 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGTATACCACAATTAT+AGG 0.240680 4_4:-46799580 Msa0683840:five_prime_UTR
AACGTATCAAGCTCCAAATT+TGG 0.259101 4_4:-46798018 Msa0683840:CDS
ACCAGTTTCATCACAACTAT+TGG 0.260192 4_4:-46798163 Msa0683840:CDS
GTCCTGCAGTATCCCAAATT+TGG 0.278971 4_4:+46798005 None:intergenic
TTCTAATGATTTCAGATATC+AGG 0.283362 4_4:-46797640 Msa0683840:intron
CAGAAAATGCAATATTGAAA+TGG 0.314871 4_4:+46795589 None:intergenic
ATCAGCCAGTGCTTGGCCTT+TGG 0.327266 4_4:+46797156 None:intergenic
TCTAACTACCTCTGTTTGAT+TGG 0.332170 4_4:-46795688 Msa0683840:three_prime_UTR
CAAGACTCTGCAACGGGGTC+TGG 0.345479 4_4:-46795924 Msa0683840:CDS
AGAAATGAATCTGTTGAAAA+TGG 0.366678 4_4:+46799280 None:intergenic
ACCTCTGTTTGATTGGGATT+AGG 0.371672 4_4:-46795681 Msa0683840:three_prime_UTR
ACGTATCAAGCTCCAAATTT+GGG 0.372566 4_4:-46798017 Msa0683840:CDS
ATAAGAACCATTGAACTTGA+TGG 0.385351 4_4:-46798043 Msa0683840:CDS
ATCAACCAAGACTCTGCAAC+GGG 0.391757 4_4:-46795930 Msa0683840:CDS
CCTTACCTGTTGTAATTGTC+CGG 0.397162 4_4:+46797972 None:intergenic
TGATTTCAGATATCAGGAAT+TGG 0.400162 4_4:-46797634 Msa0683840:intron
CCAGTTTCATCACAACTATT+GGG 0.400381 4_4:-46798162 Msa0683840:intron
TGGTGCCATGGGCATATTGC+TGG 0.408906 4_4:-46797800 Msa0683840:CDS
CAAGCACTGGCTGATGAGTA+TGG 0.410586 4_4:-46797148 Msa0683840:CDS
TGCGGCTGCAAGTCTGTATC+TGG 0.413956 4_4:-46799690 None:intergenic
TTGTGCGTGATAAGTAGTTT+CGG 0.414185 4_4:-46795757 Msa0683840:three_prime_UTR
CTTAATGAGGTAATCGTAAT+CGG 0.415779 4_4:+46799221 None:intergenic
AGTCTGTATCTGGCTTCATA+AGG 0.419229 4_4:-46799680 Msa0683840:five_prime_UTR
TAATCGGCACGAGCCCTTGC+CGG 0.419982 4_4:+46799237 None:intergenic
CTAACTACCTCTGTTTGATT+GGG 0.420571 4_4:-46795687 Msa0683840:three_prime_UTR
GGTAGGGAACAAAGCAGATA+TGG 0.426102 4_4:-46797564 Msa0683840:CDS
GGATAATGTTAACAAAATAC+TGG 0.430059 4_4:-46797585 Msa0683840:CDS
CTCATTAAGCTTCTCTTGAT+CGG 0.432261 4_4:-46799207 Msa0683840:CDS
GTCTGTATCTGGCTTCATAA+GGG 0.433509 4_4:-46799679 Msa0683840:five_prime_UTR
CCCAATAGTTGTGATGAAAC+TGG 0.436614 4_4:+46798162 None:intergenic
CTATGCGCTGATGGATGCTT+CGG 0.442040 4_4:-46795558 Msa0683840:three_prime_UTR
GTTGATTTGCTATGCGCTGA+TGG 0.442667 4_4:-46795567 Msa0683840:three_prime_UTR
CAACATTGAGCAACATGCTT+CGG 0.444470 4_4:-46797606 Msa0683840:CDS
AGAAAATGCAATATTGAAAT+GGG 0.446558 4_4:+46795590 None:intergenic
CAGATATGGACGAAAGCAAA+AGG 0.446902 4_4:-46797550 Msa0683840:CDS
ACGTTTGCCATCAAGTTCAA+TGG 0.447823 4_4:+46798036 None:intergenic
GATGGCTGCTCCACCGGCAA+GGG 0.449585 4_4:-46799250 Msa0683840:CDS
CTCTGCAACGGGGTCTGGAC+AGG 0.453418 4_4:-46795919 Msa0683840:CDS
CTGCAGGACAGGAACGGTTC+CGG 0.459289 4_4:-46797991 Msa0683840:CDS
AGCTTACTATCGTGGTGCCA+TGG 0.468786 4_4:-46797812 Msa0683840:CDS
TGATGGCTGCTCCACCGGCA+AGG 0.468923 4_4:-46799251 Msa0683840:CDS
CAGACCCCGTTGCAGAGTCT+TGG 0.475391 4_4:+46795925 None:intergenic
TGTATAAACTTGCTTATCAA+TGG 0.489406 4_4:-46795639 Msa0683840:three_prime_UTR
AAATCTGCTTGCTGTGGTTG+AGG 0.490381 4_4:-46795888 Msa0683840:exon
GCTTACTATCGTGGTGCCAT+GGG 0.506910 4_4:-46797811 Msa0683840:CDS
AACGTCCAAAGGCCAAGCAC+TGG 0.513872 4_4:-46797161 Msa0683840:CDS
AACGCATAGTTAGTAGCTTG+TGG 0.522675 4_4:+46795501 None:intergenic
CGGTTCCGGACAATTACAAC+AGG 0.522865 4_4:-46797977 Msa0683840:intron
AATGTTAACAAAATACTGGT+AGG 0.531919 4_4:-46797581 Msa0683840:CDS
AGTGCTTGGCCTTTGGACGT+TGG 0.532422 4_4:+46797163 None:intergenic
ACGCATAGTTAGTAGCTTGT+GGG 0.533460 4_4:+46795502 None:intergenic
CATACTCATCAGCCAGTGCT+TGG 0.535062 4_4:+46797149 None:intergenic
GGCATTCACTTGCGCATATG+CGG 0.545602 4_4:-46799646 Msa0683840:five_prime_UTR
CTTCTCTTGATCGGCGATAG+CGG 0.545946 4_4:-46799198 Msa0683840:intron
TAGGCTGTGCCAACGTCCAA+AGG 0.547979 4_4:-46797172 Msa0683840:CDS
AAATTCTTCGAAACTGTAAG+TGG 0.558518 4_4:-46797121 Msa0683840:CDS
CTGTATCTGGCTTCATAAGG+GGG 0.563934 4_4:-46799677 Msa0683840:five_prime_UTR
AATTTGGGATACTGCAGGAC+AGG 0.565679 4_4:-46798002 Msa0683840:CDS
CTCCAAATTTGGGATACTGC+AGG 0.565715 4_4:-46798007 Msa0683840:CDS
CAGCTTCAAAGTACAGTACA+AGG 0.570658 4_4:-46799552 Msa0683840:intron
TCGGCACGAGCCCTTGCCGG+TGG 0.580971 4_4:+46799240 None:intergenic
CTGTTTGATTGGGATTAGGA+CGG 0.583469 4_4:-46795677 Msa0683840:three_prime_UTR
TCTGTATCTGGCTTCATAAG+GGG 0.588024 4_4:-46799678 Msa0683840:five_prime_UTR
AGATATGGACGAAAGCAAAA+GGG 0.588273 4_4:-46797549 Msa0683840:intron
GCTTGCTGACACTGACAACA+AGG 0.590249 4_4:-46796960 Msa0683840:CDS
TGCAAAGACAAATCTAAATG+TGG 0.596571 4_4:-46797023 Msa0683840:CDS
AGCTGCCTCTCCTATAATTG+TGG 0.596941 4_4:+46799570 None:intergenic
ATGTTAACAAAATACTGGTA+GGG 0.601571 4_4:-46797580 Msa0683840:CDS
GGGATACTGCAGGACAGGAA+CGG 0.617575 4_4:-46797997 Msa0683840:CDS
CGATCAAGAGAAGCTTAATG+AGG 0.624299 4_4:+46799208 None:intergenic
AAAGACAAATCTAAATGTGG+AGG 0.631704 4_4:-46797020 Msa0683840:CDS
AATCAACCAAGACTCTGCAA+CGG 0.636226 4_4:-46795931 Msa0683840:CDS
TGTGATGAAACTGGTAGTGA+AGG 0.645460 4_4:+46798171 None:intergenic
ATTCACTTGCGCATATGCGG+AGG 0.646954 4_4:-46799643 Msa0683840:five_prime_UTR
TGCTTATCAATGGCGTGTAG+TGG 0.653850 4_4:-46795629 Msa0683840:three_prime_UTR
TAGCTAGAGACATAAAACAA+AGG 0.656013 4_4:-46796982 Msa0683840:CDS
TGACACTGACAACAAGGCAG+AGG 0.656933 4_4:-46796954 Msa0683840:intron
GTATACCACAATTATAGGAG+AGG 0.660299 4_4:-46799575 Msa0683840:five_prime_UTR
ATAGACCAGCAATATGCCCA+TGG 0.666814 4_4:+46797795 None:intergenic
TGTATCTGGCTTCATAAGGG+GGG 0.679081 4_4:-46799676 Msa0683840:five_prime_UTR
TCCTAATCCCAATCAAACAG+AGG 0.688148 4_4:+46795680 None:intergenic
TCAACCAAGACTCTGCAACG+GGG 0.752690 4_4:-46795929 Msa0683840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGATGAAATTAAAA+AGG + chr4_4:46795697-46795716 None:intergenic 10.0%
!! ACAAAATAAGTTTATATTAA+TGG + chr4_4:46796943-46796962 None:intergenic 10.0%
!!! TTAATTGATTTTGAAATTAA+AGG + chr4_4:46798501-46798520 None:intergenic 10.0%
!! TTAATTTCAAAATCAATTAA+TGG - chr4_4:46798501-46798520 Msa0683840:intron 10.0%
!!! AGTATCTTTTATATATATAT+GGG + chr4_4:46799123-46799142 None:intergenic 10.0%
!!! TAGTATCTTTTATATATATA+TGG + chr4_4:46799124-46799143 None:intergenic 10.0%
!! ACCATATAATAATATAAACT+AGG - chr4_4:46796048-46796067 Msa0683840:intron 15.0%
!! CCATATAATAATATAAACTA+GGG - chr4_4:46796049-46796068 Msa0683840:intron 15.0%
!!! TAATGATTAATGATAGATTT+TGG - chr4_4:46796094-46796113 Msa0683840:intron 15.0%
!! TGTTATTGTAATGTAAATTA+TGG - chr4_4:46796207-46796226 Msa0683840:intron 15.0%
!! GTATTAAACAAATAAATTGT+GGG + chr4_4:46796434-46796453 None:intergenic 15.0%
!! TGTATTAAACAAATAAATTG+TGG + chr4_4:46796435-46796454 None:intergenic 15.0%
!!! ATTTGTTATTTATCAATTGT+TGG + chr4_4:46797917-46797936 None:intergenic 15.0%
!! ATTACACAAAATATATATAG+AGG - chr4_4:46798424-46798443 Msa0683840:intron 15.0%
!! ATTGAATAATAGTAATTGTT+AGG - chr4_4:46798529-46798548 Msa0683840:intron 15.0%
!! TTGAATAATAGTAATTGTTA+GGG - chr4_4:46798530-46798549 Msa0683840:intron 15.0%
!! AAAGAAAAGAATGAAATTAT+TGG + chr4_4:46798745-46798764 None:intergenic 15.0%
!!! AATAATTTCATTCTTTTCTT+TGG - chr4_4:46798744-46798763 Msa0683840:intron 15.0%
!! TTTCAAATTATTAATTCCAT+TGG + chr4_4:46798835-46798854 None:intergenic 15.0%
!!! GTATCTTTTATATATATATG+GGG + chr4_4:46799122-46799141 None:intergenic 15.0%
!! TTATAGTATACCACAATTAT+AGG - chr4_4:46795611-46795630 Msa0683840:three_prime_UTR 20.0%
!!! ATTTTAGAGAGAGAAAATAA+CGG + chr4_4:46795775-46795794 None:intergenic 20.0%
!! CCCTAGTTTATATTATTATA+TGG + chr4_4:46796052-46796071 None:intergenic 20.0%
!!! GATTTGTTTTAAATTGTGAA+AGG - chr4_4:46796134-46796153 Msa0683840:intron 20.0%
!! CAAACAATGATTCAAATAAT+GGG + chr4_4:46796187-46796206 None:intergenic 20.0%
!! ACAAACAATGATTCAAATAA+TGG + chr4_4:46796188-46796207 None:intergenic 20.0%
!! TTTGTCATTGTCAATAATTT+GGG + chr4_4:46796321-46796340 None:intergenic 20.0%
!! TCTTTACAATAAACAGAATA+TGG - chr4_4:46796358-46796377 Msa0683840:intron 20.0%
!! ATTTGTTTAATACACAAAGT+AGG - chr4_4:46796441-46796460 Msa0683840:intron 20.0%
!! TTAAAATCAATACTGCATTA+AGG + chr4_4:46797127-46797146 None:intergenic 20.0%
!! TAATAAAAAGAAAACCTATG+AGG + chr4_4:46797253-46797272 None:intergenic 20.0%
!!! CTGCAAAAAACAAATATTTT+AGG + chr4_4:46797361-46797380 None:intergenic 20.0%
!! TTCAAAATCAATTAATGGTT+AGG - chr4_4:46798506-46798525 Msa0683840:intron 20.0%
!!! AAATTGATTTTGTCATGAAA+AGG + chr4_4:46798611-46798630 None:intergenic 20.0%
!! TGAAATTATTGGAATCTAAA+TGG + chr4_4:46798734-46798753 None:intergenic 20.0%
!! TATACTATGATAAAAAGATC+TGG - chr4_4:46798871-46798890 Msa0683840:intron 20.0%
!!! TAAATAAACTTTAAAGCTGT+TGG - chr4_4:46799027-46799046 Msa0683840:intron 20.0%
!! TGTTGGATTAAAAATAATTG+AGG - chr4_4:46799044-46799063 Msa0683840:intron 20.0%
!! GTTGGATTAAAAATAATTGA+GGG - chr4_4:46799045-46799064 Msa0683840:intron 20.0%
!! GTTTATACAGTGAAAAAAAT+GGG + chr4_4:46799541-46799560 None:intergenic 20.0%
!! AGTTTATACAGTGAAAAAAA+TGG + chr4_4:46799542-46799561 None:intergenic 20.0%
!! AGAAAATGCAATATTGAAAT+GGG + chr4_4:46799604-46799623 None:intergenic 20.0%
!! TCCTTTACTTGTGAAAATTT+TGG - chr4_4:46795803-46795822 Msa0683840:three_prime_UTR 25.0%
!! TCCAAAATTTTCACAAGTAA+AGG + chr4_4:46795807-46795826 None:intergenic 25.0%
!! TTTACTTGTGAAAATTTTGG+AGG - chr4_4:46795806-46795825 Msa0683840:three_prime_UTR 25.0%
! AGAAATGAATCTGTTGAAAA+TGG + chr4_4:46795914-46795933 None:intergenic 25.0%
! ATGGTAGAAAATACATAGAA+GGG + chr4_4:46796033-46796052 None:intergenic 25.0%
! TATGGTAGAAAATACATAGA+AGG + chr4_4:46796034-46796053 None:intergenic 25.0%
!!! TTTTGGCTTTGTTTTTGATT+TGG - chr4_4:46796111-46796130 Msa0683840:intron 25.0%
!!! TTTGGCTTTGTTTTTGATTT+GGG - chr4_4:46796112-46796131 Msa0683840:intron 25.0%
! ATGTAAATTATGGTATCAAG+AGG - chr4_4:46796217-46796236 Msa0683840:intron 25.0%
! ATTACTGCAATTGTAATGAA+CGG + chr4_4:46796298-46796317 None:intergenic 25.0%
! CTTTGTCATTGTCAATAATT+TGG + chr4_4:46796322-46796341 None:intergenic 25.0%
! ATACTTCATTATGTAAACCA+AGG + chr4_4:46796864-46796883 None:intergenic 25.0%
! AGCAACACAGTTATTTAAAT+TGG + chr4_4:46796895-46796914 None:intergenic 25.0%
!!! ATTTTGTTATTGTTCTTCAC+AGG - chr4_4:46796956-46796975 Msa0683840:CDS 25.0%
!! AACTATTGGGTATGATTTTA+TGG - chr4_4:46797042-46797061 Msa0683840:CDS 25.0%
!! ATCTGAAATCATTAGAAGAT+AGG + chr4_4:46797548-46797567 None:intergenic 25.0%
! TTCTAATGATTTCAGATATC+AGG - chr4_4:46797551-46797570 Msa0683840:CDS 25.0%
! GGATAATGTTAACAAAATAC+TGG - chr4_4:46797606-46797625 Msa0683840:CDS 25.0%
! AATGTTAACAAAATACTGGT+AGG - chr4_4:46797610-46797629 Msa0683840:CDS 25.0%
! ATGTTAACAAAATACTGGTA+GGG - chr4_4:46797611-46797630 Msa0683840:CDS 25.0%
!! TAAAGGTTGATTTACAAAGA+GGG + chr4_4:46797738-46797757 None:intergenic 25.0%
!! TAAACATTTTTGTTGACGAA+CGG - chr4_4:46797857-46797876 Msa0683840:intron 25.0%
!! GTACTTTTACCTTTCTTTTT+AGG - chr4_4:46798000-46798019 Msa0683840:CDS 25.0%
! ATACAGAGTCGATATTATAT+AGG - chr4_4:46798383-46798402 Msa0683840:intron 25.0%
! ACACAAAATATATATAGAGG+AGG - chr4_4:46798427-46798446 Msa0683840:intron 25.0%
! CACAAAATATATATAGAGGA+GGG - chr4_4:46798428-46798447 Msa0683840:intron 25.0%
! TAAGATTATAGACTTACTCT+TGG + chr4_4:46798465-46798484 None:intergenic 25.0%
! TGAAATTAAAGGTCATGATT+TGG + chr4_4:46798490-46798509 None:intergenic 25.0%
!!! TAACCAAAGAAGTACATTTT+GGG + chr4_4:46798706-46798725 None:intergenic 25.0%
!!! TTAACCAAAGAAGTACATTT+TGG + chr4_4:46798707-46798726 None:intergenic 25.0%
!! CTTTTATATATATATGGGGA+GGG + chr4_4:46799118-46799137 None:intergenic 25.0%
!! TCTTTTATATATATATGGGG+AGG + chr4_4:46799119-46799138 None:intergenic 25.0%
! AATATCGTATCGTGTAAATA+AGG + chr4_4:46799148-46799167 None:intergenic 25.0%
!!! AAAAGGTATCACAAAACATT+AGG + chr4_4:46799219-46799238 None:intergenic 25.0%
!! TGTATAAACTTGCTTATCAA+TGG - chr4_4:46799552-46799571 Msa0683840:intron 25.0%
! CAGAAAATGCAATATTGAAA+TGG + chr4_4:46799605-46799624 None:intergenic 25.0%
! TTTTCACAAGTAAAGGAACA+TGG + chr4_4:46795800-46795819 None:intergenic 30.0%
! GATTCATTTCTTCGTTTTGA+TGG - chr4_4:46795923-46795942 Msa0683840:CDS 30.0%
CTTAATGAGGTAATCGTAAT+CGG + chr4_4:46795973-46795992 None:intergenic 30.0%
TGGTAGAAAATACATAGAAG+GGG + chr4_4:46796032-46796051 None:intergenic 30.0%
! TGAAAGGTTTGATCTTTTCA+TGG - chr4_4:46796150-46796169 Msa0683840:intron 30.0%
TAATGAACGGATCACATAAA+AGG + chr4_4:46796285-46796304 None:intergenic 30.0%
GTAATTGAGATCAACTAAAG+GGG + chr4_4:46796580-46796599 None:intergenic 30.0%
GGTAATTGAGATCAACTAAA+GGG + chr4_4:46796581-46796600 None:intergenic 30.0%
AGGTAATTGAGATCAACTAA+AGG + chr4_4:46796582-46796601 None:intergenic 30.0%
ACCTTCTTGTGTAAAATACA+GGG - chr4_4:46796674-46796693 Msa0683840:intron 30.0%
TGTACAATACACTAATTGGT+GGG - chr4_4:46796705-46796724 Msa0683840:intron 30.0%
TGTGATATTAGAGAAATCTC+AGG + chr4_4:46797098-46797117 None:intergenic 30.0%
ATAAGAACCATTGAACTTGA+TGG - chr4_4:46797148-46797167 Msa0683840:CDS 30.0%
TGATTTCAGATATCAGGAAT+TGG - chr4_4:46797557-46797576 Msa0683840:CDS 30.0%
! AAAGGTTGATTTACAAAGAG+GGG + chr4_4:46797737-46797756 None:intergenic 30.0%
! GTAAAGGTTGATTTACAAAG+AGG + chr4_4:46797739-46797758 None:intergenic 30.0%
AGTACATTTGACAACTGTAA+AGG + chr4_4:46797755-46797774 None:intergenic 30.0%
GTAAATTTGCATGACGATTA+TGG + chr4_4:46797779-46797798 None:intergenic 30.0%
TAATCGTCATGCAAATTTAC+TGG - chr4_4:46797779-46797798 Msa0683840:CDS 30.0%
AAGGTTGAATTGCATTGTTA+AGG - chr4_4:46797802-46797821 Msa0683840:CDS 30.0%
! AAACATTTTTGTTGACGAAC+GGG - chr4_4:46797858-46797877 Msa0683840:intron 30.0%
AAATTCTTCGAAACTGTAAG+TGG - chr4_4:46798070-46798089 Msa0683840:intron 30.0%
! GTGTATTTTACCTGAAAATG+TGG - chr4_4:46798092-46798111 Msa0683840:intron 30.0%
TGCAAAGACAAATCTAAATG+TGG - chr4_4:46798168-46798187 Msa0683840:CDS 30.0%
AAAGACAAATCTAAATGTGG+AGG - chr4_4:46798171-46798190 Msa0683840:CDS 30.0%
!! TTGTTTTATGTCTCTAGCTA+TGG + chr4_4:46798209-46798228 None:intergenic 30.0%
TAGCTAGAGACATAAAACAA+AGG - chr4_4:46798209-46798228 Msa0683840:CDS 30.0%
TTAGAGACATGAAACTATGA+CGG + chr4_4:46798299-46798318 None:intergenic 30.0%
GAGAAAAGTAGAAAGACATA+TGG - chr4_4:46798342-46798361 Msa0683840:intron 30.0%
TAATAGTAATTGTTAGGGTG+AGG - chr4_4:46798535-46798554 Msa0683840:intron 30.0%
TTAGGTAATCCCAATTTAAG+AGG - chr4_4:46798782-46798801 Msa0683840:intron 30.0%
!!! TGATTTGTTTTTGTCGTTTG+CGG - chr4_4:46798944-46798963 Msa0683840:intron 30.0%
! ATTTTGTTGAGTGTGTTTAG+AGG - chr4_4:46798979-46798998 Msa0683840:intron 30.0%
TAAGTTTAGAGACTTACTCT+TGG - chr4_4:46799081-46799100 Msa0683840:intron 30.0%
GTATACCACAATTATAGGAG+AGG - chr4_4:46795616-46795635 Msa0683840:three_prime_UTR 35.0%
CTCATTAAGCTTCTCTTGAT+CGG - chr4_4:46795984-46796003 Msa0683840:intron 35.0%
GGTAGAAAATACATAGAAGG+GGG + chr4_4:46796031-46796050 None:intergenic 35.0%
TGGATCGAATTCTTACCAAA+TGG + chr4_4:46796506-46796525 None:intergenic 35.0%
ACTAGTAGTCTAGTACGTAT+AGG - chr4_4:46796528-46796547 Msa0683840:intron 35.0%
CACCTTCTTGTGTAAAATAC+AGG - chr4_4:46796673-46796692 Msa0683840:intron 35.0%
! ACCCTGTATTTTACACAAGA+AGG + chr4_4:46796678-46796697 None:intergenic 35.0%
CTTGTGTAAAATACAGGGTA+AGG - chr4_4:46796679-46796698 Msa0683840:intron 35.0%
GCTGTGTACAATACACTAAT+TGG - chr4_4:46796701-46796720 Msa0683840:intron 35.0%
GTGTACAATACACTAATTGG+TGG - chr4_4:46796704-46796723 Msa0683840:intron 35.0%
GTAATTGAGATCACCTAAAG+GGG + chr4_4:46796792-46796811 None:intergenic 35.0%
GGTAATTGAGATCACCTAAA+GGG + chr4_4:46796793-46796812 None:intergenic 35.0%
AGGTAATTGAGATCACCTAA+AGG + chr4_4:46796794-46796813 None:intergenic 35.0%
! GCTTTTGTGTGAAACATACT+TGG - chr4_4:46796912-46796931 Msa0683840:intron 35.0%
TTATTGTTCTTCACAGGTGT+TGG - chr4_4:46796962-46796981 Msa0683840:CDS 35.0%
! CTTCTTTTGCGTTTTTCTGA+TGG - chr4_4:46796995-46797014 Msa0683840:CDS 35.0%
ACCAGTTTCATCACAACTAT+TGG - chr4_4:46797028-46797047 Msa0683840:CDS 35.0%
CCAGTTTCATCACAACTATT+GGG - chr4_4:46797029-46797048 Msa0683840:CDS 35.0%
AACGTATCAAGCTCCAAATT+TGG - chr4_4:46797173-46797192 Msa0683840:intron 35.0%
ACGTATCAAGCTCCAAATTT+GGG - chr4_4:46797174-46797193 Msa0683840:intron 35.0%
GTAAGGTTTATGATCCTCAT+AGG - chr4_4:46797236-46797255 Msa0683840:intron 35.0%
!!! TTTTTTGCAGCTTACTATCG+TGG - chr4_4:46797371-46797390 Msa0683840:intron 35.0%
AACAGTAATGTTCCTTTCTG+TGG - chr4_4:46797443-46797462 Msa0683840:intron 35.0%
TTCAAACTAAAGCCACAGAA+AGG + chr4_4:46797458-46797477 None:intergenic 35.0%
! TGTCTTTTTCTTCCTTTCCA+TGG - chr4_4:46797513-46797532 Msa0683840:intron 35.0%
! GTCTTTTTCTTCCTTTCCAT+GGG - chr4_4:46797514-46797533 Msa0683840:intron 35.0%
AGATATGGACGAAAGCAAAA+GGG - chr4_4:46797642-46797661 Msa0683840:intron 35.0%
! AAAGGGTATGCAAACTTTGT+TGG - chr4_4:46797659-46797678 Msa0683840:intron 35.0%
! AAGGTTGATTTACAAAGAGG+GGG + chr4_4:46797736-46797755 None:intergenic 35.0%
! AACATTTTTGTTGACGAACG+GGG - chr4_4:46797859-46797878 Msa0683840:intron 35.0%
TAGAGACATGAAACTATGAC+GGG + chr4_4:46798298-46798317 None:intergenic 35.0%
GACTCTGTATTTCGAAAACA+CGG + chr4_4:46798373-46798392 None:intergenic 35.0%
!! GGTTTAATTGTTTTGTCCAC+TGG - chr4_4:46798556-46798575 Msa0683840:intron 35.0%
GATCCCAAAATGTACTTCTT+TGG - chr4_4:46798700-46798719 Msa0683840:intron 35.0%
AGCATCTCACCTCTTAAATT+GGG + chr4_4:46798794-46798813 None:intergenic 35.0%
AAGCATCTCACCTCTTAAAT+TGG + chr4_4:46798795-46798814 None:intergenic 35.0%
AATTTAAGAGGTGAGATGCT+TGG - chr4_4:46798794-46798813 Msa0683840:intron 35.0%
ATTTAAGAGGTGAGATGCTT+GGG - chr4_4:46798795-46798814 Msa0683840:intron 35.0%
GGGATAATTATTGTAGCCAA+TGG - chr4_4:46798816-46798835 Msa0683840:intron 35.0%
AATAATTGAGGGCTGAGATT+TGG - chr4_4:46799056-46799075 Msa0683840:intron 35.0%
!! TGGTTGATTTTGATGGTTGT+TGG + chr4_4:46799249-46799268 None:intergenic 35.0%
!! AGAGTCTTGGTTGATTTTGA+TGG + chr4_4:46799256-46799275 None:intergenic 35.0%
! TTGAGGACAGTTTTACAAGT+TGG - chr4_4:46799320-46799339 Msa0683840:intron 35.0%
TTGTGCGTGATAAGTAGTTT+CGG - chr4_4:46799434-46799453 Msa0683840:intron 35.0%
TCTAACTACCTCTGTTTGAT+TGG - chr4_4:46799503-46799522 Msa0683840:intron 35.0%
CTAACTACCTCTGTTTGATT+GGG - chr4_4:46799504-46799523 Msa0683840:intron 35.0%
! AGTCTGTATCTGGCTTCATA+AGG - chr4_4:46795511-46795530 Msa0683840:three_prime_UTR 40.0%
! GTCTGTATCTGGCTTCATAA+GGG - chr4_4:46795512-46795531 Msa0683840:three_prime_UTR 40.0%
! TCTGTATCTGGCTTCATAAG+GGG - chr4_4:46795513-46795532 Msa0683840:three_prime_UTR 40.0%
CAGCTTCAAAGTACAGTACA+AGG - chr4_4:46795639-46795658 Msa0683840:three_prime_UTR 40.0%
CGATCAAGAGAAGCTTAATG+AGG + chr4_4:46795986-46796005 None:intergenic 40.0%
TACACAAAGTAGGCCTTAAG+CGG - chr4_4:46796451-46796470 Msa0683840:intron 40.0%
GTTTCGGTCTCTTAACCATT+TGG - chr4_4:46796488-46796507 Msa0683840:intron 40.0%
ACGTACTAGACTACTAGTCA+TGG + chr4_4:46796526-46796545 None:intergenic 40.0%
TTGATCTCAATTACCTCGAG+TGG - chr4_4:46796586-46796605 Msa0683840:intron 40.0%
!! TTACACAAGAAGGTGTTTCC+AGG + chr4_4:46796668-46796687 None:intergenic 40.0%
! TGTGATGAAACTGGTAGTGA+AGG + chr4_4:46797023-46797042 None:intergenic 40.0%
CCCAATAGTTGTGATGAAAC+TGG + chr4_4:46797032-46797051 None:intergenic 40.0%
ACGTTTGCCATCAAGTTCAA+TGG + chr4_4:46797158-46797177 None:intergenic 40.0%
CCTTACCTGTTGTAATTGTC+CGG + chr4_4:46797222-46797241 None:intergenic 40.0%
GAAACACACTGTTTGACTGT+TGG - chr4_4:46797306-46797325 Msa0683840:intron 40.0%
AAGATAGGTGAATACTCCCA+TGG + chr4_4:46797533-46797552 None:intergenic 40.0%
CAACATTGAGCAACATGCTT+CGG - chr4_4:46797585-46797604 Msa0683840:CDS 40.0%
CAGATATGGACGAAAGCAAA+AGG - chr4_4:46797641-46797660 Msa0683840:intron 40.0%
! AGGTTGATTTACAAAGAGGG+GGG + chr4_4:46797735-46797754 None:intergenic 40.0%
CGTCATGCAAATTTACTGGA+AGG - chr4_4:46797783-46797802 Msa0683840:CDS 40.0%
TTAGTTCACTCACAGCAACA+TGG + chr4_4:46797840-46797859 None:intergenic 40.0%
! AAGAGGGTTGAGTTCTGATT+TGG - chr4_4:46797889-46797908 Msa0683840:intron 40.0%
TTGGCACAGCCTAAAAAGAA+AGG + chr4_4:46798012-46798031 None:intergenic 40.0%
! CAGAGAGTTTCCACATTTTC+AGG + chr4_4:46798105-46798124 None:intergenic 40.0%
CTCATTCAACATGAGAGAAG+AGG + chr4_4:46798323-46798342 None:intergenic 40.0%
TGGATGAACGCAGAATTCTT+AGG - chr4_4:46798764-46798783 Msa0683840:intron 40.0%
TTTAAGAGGTGAGATGCTTG+GGG - chr4_4:46798796-46798815 Msa0683840:intron 40.0%
TGTTGAGTGTGTTTAGAGGA+AGG - chr4_4:46798983-46799002 Msa0683840:intron 40.0%
AACATTAGGCTCGTGAGAAA+GGG + chr4_4:46799205-46799224 None:intergenic 40.0%
AAACATTAGGCTCGTGAGAA+AGG + chr4_4:46799206-46799225 None:intergenic 40.0%
! TGGTTGTTGGCTGCAATAAA+AGG + chr4_4:46799236-46799255 None:intergenic 40.0%
AATCAACCAAGACTCTGCAA+CGG - chr4_4:46799260-46799279 Msa0683840:exon 40.0%
! TGTGCTCAACGTTTTCAATG+TGG - chr4_4:46799396-46799415 Msa0683840:intron 40.0%
ACCTCTGTTTGATTGGGATT+AGG - chr4_4:46799510-46799529 Msa0683840:intron 40.0%
TCCTAATCCCAATCAAACAG+AGG + chr4_4:46799514-46799533 None:intergenic 40.0%
CTGTTTGATTGGGATTAGGA+CGG - chr4_4:46799514-46799533 Msa0683840:intron 40.0%
! CTGTATCTGGCTTCATAAGG+GGG - chr4_4:46795514-46795533 Msa0683840:three_prime_UTR 45.0%
! TGTATCTGGCTTCATAAGGG+GGG - chr4_4:46795515-46795534 Msa0683840:three_prime_UTR 45.0%
AGCTGCCTCTCCTATAATTG+TGG + chr4_4:46795624-46795643 None:intergenic 45.0%
GGTCCAAGTGGTAGATGTTT+CGG - chr4_4:46796472-46796491 Msa0683840:intron 45.0%
AGACCGAAACATCTACCACT+TGG + chr4_4:46796478-46796497 None:intergenic 45.0%
TCGCTTATCACGTGACTGAA+AGG - chr4_4:46796628-46796647 Msa0683840:intron 45.0%
CCTAAAGGGGCTGACATAAA+GGG + chr4_4:46796779-46796798 None:intergenic 45.0%
ACCTAAAGGGGCTGACATAA+AGG + chr4_4:46796780-46796799 None:intergenic 45.0%
GTGATCTCAATTACCTCGAG+TGG - chr4_4:46796798-46796817 Msa0683840:intron 45.0%
! CTCCAAATTTGGGATACTGC+AGG - chr4_4:46797184-46797203 Msa0683840:intron 45.0%
GTCCTGCAGTATCCCAAATT+TGG + chr4_4:46797189-46797208 None:intergenic 45.0%
! AATTTGGGATACTGCAGGAC+AGG - chr4_4:46797189-46797208 Msa0683840:intron 45.0%
CCGGACAATTACAACAGGTA+AGG - chr4_4:46797219-46797238 Msa0683840:intron 45.0%
ACTGTTTGACTGTTGGCATC+TGG - chr4_4:46797313-46797332 Msa0683840:intron 45.0%
ATAGACCAGCAATATGCCCA+TGG + chr4_4:46797399-46797418 None:intergenic 45.0%
AGGTGAATACTCCCATGGAA+AGG + chr4_4:46797528-46797547 None:intergenic 45.0%
GGTAGGGAACAAAGCAGATA+TGG - chr4_4:46797627-46797646 Msa0683840:CDS 45.0%
! GGTTGATTTACAAAGAGGGG+GGG + chr4_4:46797734-46797753 None:intergenic 45.0%
! ATTTTTGTTGACGAACGGGG+AGG - chr4_4:46797862-46797881 Msa0683840:intron 45.0%
ACACACATATACGAGAGAGG+AGG + chr4_4:46797945-46797964 None:intergenic 45.0%
GACACACACATATACGAGAG+AGG + chr4_4:46797948-46797967 None:intergenic 45.0%
! TTGCAGCAACAAGCGTTTTG+CGG + chr4_4:46798586-46798605 None:intergenic 45.0%
GTGTGTTTAGAGGAAGGAAG+CGG - chr4_4:46798989-46799008 Msa0683840:intron 45.0%
ATCAACCAAGACTCTGCAAC+GGG - chr4_4:46799261-46799280 Msa0683840:exon 45.0%
! GCACAAAAATCTGCTTGCTG+TGG - chr4_4:46799297-46799316 Msa0683840:five_prime_UTR 45.0%
!! AAATCTGCTTGCTGTGGTTG+AGG - chr4_4:46799303-46799322 Msa0683840:intron 45.0%
TGCTTATCAATGGCGTGTAG+TGG - chr4_4:46799562-46799581 Msa0683840:five_prime_UTR 45.0%
!! GTTGATTTGCTATGCGCTGA+TGG - chr4_4:46799624-46799643 Msa0683840:five_prime_UTR 45.0%
GGCATTCACTTGCGCATATG+CGG - chr4_4:46795545-46795564 Msa0683840:three_prime_UTR 50.0%
ATTCACTTGCGCATATGCGG+AGG - chr4_4:46795548-46795567 Msa0683840:three_prime_UTR 50.0%
AAGGAGAGAGAGAGAGAGAG+AGG + chr4_4:46795678-46795697 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAAG+TGG + chr4_4:46795836-46795855 None:intergenic 50.0%
CTTCTCTTGATCGGCGATAG+CGG - chr4_4:46795993-46796012 Msa0683840:intron 50.0%
TCTACCACTTGGACCGCTTA+AGG + chr4_4:46796467-46796486 None:intergenic 50.0%
CTTTACTGTTGCACCACTCG+AGG + chr4_4:46796602-46796621 None:intergenic 50.0%
AAGGTCACGAGTTCAAGTCC+TGG - chr4_4:46796647-46796666 Msa0683840:intron 50.0%
CACTAAAGCTCCCGCATACA+CGG + chr4_4:46796746-46796765 None:intergenic 50.0%
! ATGCGGGAGCTTTAGTGCAT+CGG - chr4_4:46796749-46796768 Msa0683840:intron 50.0%
CCCTTTATGTCAGCCCCTTT+AGG - chr4_4:46796776-46796795 Msa0683840:intron 50.0%
CGGTTCCGGACAATTACAAC+AGG - chr4_4:46797214-46797233 Msa0683840:intron 50.0%
! AGCTTACTATCGTGGTGCCA+TGG - chr4_4:46797379-46797398 Msa0683840:intron 50.0%
!! GCTTACTATCGTGGTGCCAT+GGG - chr4_4:46797380-46797399 Msa0683840:intron 50.0%
AAAGAGGGGGGGAACGAAAA+AGG + chr4_4:46797723-46797742 None:intergenic 50.0%
CATACTCATCAGCCAGTGCT+TGG + chr4_4:46798045-46798064 None:intergenic 50.0%
!! CAAGCACTGGCTGATGAGTA+TGG - chr4_4:46798043-46798062 Msa0683840:CDS 50.0%
! GCTTGCTGACACTGACAACA+AGG - chr4_4:46798231-46798250 Msa0683840:intron 50.0%
TGACACTGACAACAAGGCAG+AGG - chr4_4:46798237-46798256 Msa0683840:intron 50.0%
! AGCGTTTTGCGGTATTCCAG+TGG + chr4_4:46798575-46798594 None:intergenic 50.0%
AGAGGAAGGAAGCGGAAAAG+TGG - chr4_4:46798997-46799016 Msa0683840:intron 50.0%
TCAACCAAGACTCTGCAACG+GGG - chr4_4:46799262-46799281 Msa0683840:exon 50.0%
! CTATGCGCTGATGGATGCTT+CGG - chr4_4:46799633-46799652 Msa0683840:five_prime_UTR 50.0%
CTTCATAAGGGGGGAGTTGC+TGG - chr4_4:46795524-46795543 Msa0683840:three_prime_UTR 55.0%
!! CGTTTTGATGGCTGCTCCAC+CGG - chr4_4:46795935-46795954 Msa0683840:CDS 55.0%
TAGGCCTTAAGCGGTCCAAG+TGG - chr4_4:46796460-46796479 Msa0683840:intron 55.0%
! TGCGGGAGCTTTAGTGCATC+GGG - chr4_4:46796750-46796769 Msa0683840:intron 55.0%
GCAGACACACTAACCACTCG+AGG + chr4_4:46796814-46796833 None:intergenic 55.0%
CGTTGCACGCTGAGATACCT+TGG - chr4_4:46796844-46796863 Msa0683840:intron 55.0%
GGGATACTGCAGGACAGGAA+CGG - chr4_4:46797194-46797213 Msa0683840:intron 55.0%
!! TGGTGCCATGGGCATATTGC+TGG - chr4_4:46797391-46797410 Msa0683840:intron 55.0%
TAGGCTGTGCCAACGTCCAA+AGG - chr4_4:46798019-46798038 Msa0683840:CDS 55.0%
! AGTGCTTGGCCTTTGGACGT+TGG + chr4_4:46798031-46798050 None:intergenic 55.0%
AACGTCCAAAGGCCAAGCAC+TGG - chr4_4:46798030-46798049 Msa0683840:CDS 55.0%
! ATCAGCCAGTGCTTGGCCTT+TGG + chr4_4:46798038-46798057 None:intergenic 55.0%
TAATCGGCACGAGCCCTTGC+CGG + chr4_4:46795957-46795976 None:intergenic 60.0%
CATACACGGCGTCTGGGAAG+GGG + chr4_4:46796732-46796751 None:intergenic 60.0%
GCATACACGGCGTCTGGGAA+GGG + chr4_4:46796733-46796752 None:intergenic 60.0%
CTTCCCAGACGCCGTGTATG+CGG - chr4_4:46796732-46796751 Msa0683840:intron 60.0%
TTCCCAGACGCCGTGTATGC+GGG - chr4_4:46796733-46796752 Msa0683840:intron 60.0%
CTGCAGGACAGGAACGGTTC+CGG - chr4_4:46797200-46797219 Msa0683840:intron 60.0%
GGGGGAACGAAAAAGGCAGG+AGG + chr4_4:46797716-46797735 None:intergenic 60.0%
AGGGGGGGAACGAAAAAGGC+AGG + chr4_4:46797719-46797738 None:intergenic 60.0%
ACGAACGGGGAGGTTCGAAG+AGG - chr4_4:46797872-46797891 Msa0683840:intron 60.0%
CGAACGGGGAGGTTCGAAGA+GGG - chr4_4:46797873-46797892 Msa0683840:intron 60.0%
CAGACCCCGTTGCAGAGTCT+TGG + chr4_4:46799269-46799288 None:intergenic 60.0%
CAAGACTCTGCAACGGGGTC+TGG - chr4_4:46799267-46799286 Msa0683840:exon 60.0%
TGATGGCTGCTCCACCGGCA+AGG - chr4_4:46795940-46795959 Msa0683840:CDS 65.0%
GATGGCTGCTCCACCGGCAA+GGG - chr4_4:46795941-46795960 Msa0683840:CDS 65.0%
CGCATACACGGCGTCTGGGA+AGG + chr4_4:46796734-46796753 None:intergenic 65.0%
CTCCCGCATACACGGCGTCT+GGG + chr4_4:46796738-46796757 None:intergenic 65.0%
CTCTGCAACGGGGTCTGGAC+AGG - chr4_4:46799272-46799291 Msa0683840:five_prime_UTR 65.0%
GCTCCCGCATACACGGCGTC+TGG + chr4_4:46796739-46796758 None:intergenic 70.0%
TCGGCACGAGCCCTTGCCGG+TGG + chr4_4:46795954-46795973 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 46795509 46799704 46795509 ID=Msa0683840;Name=Msa0683840
chr4_4 mRNA 46795509 46799704 46795509 ID=Msa0683840-mRNA-1;Parent=Msa0683840;Name=Msa0683840-mRNA-1;_AED=0.11;_eAED=0.11;_QI=197|1|0.87|1|0.85|0.75|8|381|241
chr4_4 exon 46795509 46795967 46795509 ID=Msa0683840-mRNA-1:exon:11552;Parent=Msa0683840-mRNA-1
chr4_4 exon 46796955 46797191 46796955 ID=Msa0683840-mRNA-1:exon:11551;Parent=Msa0683840-mRNA-1
chr4_4 exon 46797550 46797647 46797550 ID=Msa0683840-mRNA-1:exon:11550;Parent=Msa0683840-mRNA-1
chr4_4 exon 46797767 46797832 46797767 ID=Msa0683840-mRNA-1:exon:11549;Parent=Msa0683840-mRNA-1
chr4_4 exon 46797978 46798078 46797978 ID=Msa0683840-mRNA-1:exon:11548;Parent=Msa0683840-mRNA-1
chr4_4 exon 46798163 46798235 46798163 ID=Msa0683840-mRNA-1:exon:11547;Parent=Msa0683840-mRNA-1
chr4_4 exon 46799199 46799316 46799199 ID=Msa0683840-mRNA-1:exon:11546;Parent=Msa0683840-mRNA-1
chr4_4 exon 46799553 46799704 46799553 ID=Msa0683840-mRNA-1:exon:11545;Parent=Msa0683840-mRNA-1
chr4_4 five_prime_UTR 46799553 46799704 46799553 ID=Msa0683840-mRNA-1:five_prime_utr;Parent=Msa0683840-mRNA-1
chr4_4 five_prime_UTR 46799272 46799316 46799272 ID=Msa0683840-mRNA-1:five_prime_utr;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46799199 46799271 46799199 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46798163 46798235 46798163 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46797978 46798078 46797978 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46797767 46797832 46797767 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46797550 46797647 46797550 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46796955 46797191 46796955 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 CDS 46795890 46795967 46795890 ID=Msa0683840-mRNA-1:cds;Parent=Msa0683840-mRNA-1
chr4_4 three_prime_UTR 46795509 46795889 46795509 ID=Msa0683840-mRNA-1:three_prime_utr;Parent=Msa0683840-mRNA-1
Gene Sequence

>Msa0683840

ATGGCTGCTCCACCGGCAAGGGCTCGTGCCGATTACGATTACCTCATTAAGCTTCTCTTGATCGGCGATAGCGGTGTTGGAAAAAGTTGTCTTCTTTTGCGTTTTTCTGATGGTTCCTTCACTACCAGTTTCATCACAACTATTGGTATTGATTTTAAGATAAGAACCATTGAACTTGATGGCAAACGTATCAAGCTCCAAATTTGGGATACTGCAGGACAGGAACGGTTCCGGACAATTACAACAGCTTACTATCGTGGTGCCATGGGCATATTGCTGGTCTATGATGTTACTGATGAAGCATCATTTAACAATATCAGGAATTGGATTCGCAACATTGAGCAACATGCTTCGGATAATGTTAACAAAATACTGGTAGGGAACAAAGCAGATATGGACGAAAGCAAAAGGGCTGTGCCAACGTCCAAAGGCCAAGCACTGGCTGATGAGTATGGAATCAAATTCTTCGAAACTGTAAGTGGTGTATTTTACCTGAAAATGTGGAAACTCTCTGAACTTCTATGTTGTTATGCTCATGAAAATGCTCAATTGCAGAGTGCAAAGACAAATCTAAATGTGGAGGAAGTTTTCTTTTCCATAGCTAGAGACATAAAACAAAGGCTTGCTGACACTGACAACAAGGCAGAGCCAACAACCATCAAAATCAACCAAGACTCTGCAACGGGGTCTGGACAGGCTGCACAAAAATCTGCTTGCTGTGGTTGA
Protein sequence

>Msa0683840

MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEASFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETVSGVFYLKMWKLSELLCCYAHENAQLQSAKTNLNVEEVFFSIARDIKQRLADTDNKAEPTTIKINQDSATGSGQAAQKSACCG*