Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045790 | Msa0709010 | 0.803259 | 3.797245e-49 | -8.615850e-47 |
| Msa0046160 | Msa0709010 | 0.802719 | 4.908799e-49 | -8.615850e-47 |
| Msa0701830 | Msa0709010 | 0.927404 | 1.232460e-91 | -8.615850e-47 |
| Msa0703360 | Msa0709010 | 0.922470 | 9.429164e-89 | -8.615850e-47 |
| Msa0709010 | Msa0717890 | 0.810562 | 1.083814e-50 | -8.615850e-47 |
| Msa0709010 | Msa0774160 | 0.962016 | 2.234705e-120 | -8.615850e-47 |
| Msa0709010 | Msa0880950 | 0.805277 | 1.442706e-49 | -8.615850e-47 |
| Msa0709010 | Msa0941190 | 0.818726 | 1.686720e-52 | -8.615850e-47 |
| Msa0709010 | Msa1004800 | 0.805899 | 1.068296e-49 | -8.615850e-47 |
| Msa0709010 | Msa1004840 | 0.831534 | 1.581073e-55 | -8.615850e-47 |
| Msa0709010 | Msa1035480 | 0.806686 | 7.291375e-50 | -8.615850e-47 |
| Msa0709010 | Msa1075540 | 0.805234 | 1.473018e-49 | -8.615850e-47 |
| Msa0709010 | Msa1075650 | 0.804358 | 2.244168e-49 | -8.615850e-47 |
| Msa0709010 | Msa1083840 | 0.804239 | 2.376882e-49 | -8.615850e-47 |
| Msa0709010 | Msa1101090 | 0.828996 | 6.588324e-55 | -8.615850e-47 |
| Msa0709010 | Msa1108760 | 0.801749 | 7.774338e-49 | -8.615850e-47 |
| Msa0709010 | Msa1163090 | 0.805851 | 1.093161e-49 | -8.615850e-47 |
| Msa0709010 | Msa1186020 | 0.815481 | 9.042685e-52 | -8.615850e-47 |
| Msa0709010 | Msa1232700 | 0.816652 | 4.951289e-52 | -8.615850e-47 |
| Msa0709010 | Msa1297230 | 0.811168 | 8.010176e-51 | -8.615850e-47 |
| Msa0709010 | Msa1308870 | 0.804579 | 2.018673e-49 | -8.615850e-47 |
| Msa0709010 | Msa1313280 | 0.821301 | 4.341649e-53 | -8.615850e-47 |
| Msa0709010 | Msa1331000 | 0.800605 | 1.332856e-48 | -8.615850e-47 |
| Msa0709010 | Msa1343430 | 0.809337 | 1.988991e-50 | -8.615850e-47 |
| Msa0709010 | Msa1373030 | 0.800792 | 1.220436e-48 | -8.615850e-47 |
| Msa0709010 | Msa1409700 | 0.801758 | 7.742724e-49 | -8.615850e-47 |
| Msa0709010 | Msa1433810 | 0.801848 | 7.418398e-49 | -8.615850e-47 |
| Msa0709010 | Msa1436480 | 0.803423 | 3.511261e-49 | -8.615850e-47 |
| Msa0709010 | Msa1440560 | 0.805908 | 1.063647e-49 | -8.615850e-47 |
| Msa0709010 | Msa1463900 | 0.801042 | 1.085141e-48 | -8.615850e-47 |
| Msa0709010 | Msa1464810 | 0.812409 | 4.301803e-51 | -8.615850e-47 |
| Msa0211150 | Msa0709010 | 0.801214 | 1.000527e-48 | -8.615850e-47 |
| Msa0221470 | Msa0709010 | 0.808713 | 2.704885e-50 | -8.615850e-47 |
| Msa0225100 | Msa0709010 | 0.804984 | 1.661682e-49 | -8.615850e-47 |
| Msa0225860 | Msa0709010 | 0.803599 | 3.228309e-49 | -8.615850e-47 |
| Msa0386090 | Msa0709010 | 0.815409 | 9.380784e-52 | -8.615850e-47 |
| Msa0287740 | Msa0709010 | 0.805896 | 1.069585e-49 | -8.615850e-47 |
| Msa0326940 | Msa0709010 | 0.927631 | 8.977338e-92 | -8.615850e-47 |
| Msa0531180 | Msa0709010 | 0.801399 | 9.171905e-49 | -8.615850e-47 |
| Msa0585560 | Msa0709010 | 0.802731 | 4.880782e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTTCAGGCGACAGTTTA+AGG | 0.122983 | 4_4:-82838638 | None:intergenic |
| TAATTAATGTATGTGACTTT+TGG | 0.258825 | 4_4:+82839592 | Msa0709010:three_prime_UTR |
| CATTGCTGTAAAACGTGTTT+TGG | 0.309510 | 4_4:-82839507 | None:intergenic |
| AGAAATTACTCGTCTTCATC+AGG | 0.431687 | 4_4:+82838561 | Msa0709010:five_prime_UTR |
| AAGTTTGAGTAATTTAAAGA+AGG | 0.477272 | 4_4:+82839530 | Msa0709010:three_prime_UTR |
| ATCTTATTTGAAAGATTGAA+AGG | 0.529072 | 4_4:+82839378 | Msa0709010:intron |
| AGGCAATCAATACAACTTAG+TGG | 0.556871 | 4_4:-82839642 | None:intergenic |
| TGAAGAGTGTGTGGGAGAGA+AGG | 0.573340 | 4_4:+82838668 | Msa0709010:CDS |
| TTGCCTCAATTATCACGTGA+AGG | 0.577241 | 4_4:+82839659 | Msa0709010:three_prime_UTR |
| CAACCTTCACGTGATAATTG+AGG | 0.587359 | 4_4:-82839662 | None:intergenic |
| GAATACAAACAATTGCAGAA+AGG | 0.599398 | 4_4:+82839417 | Msa0709010:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTCAAACATTTTTTTA+AGG | - | chr4_4:82838738-82838757 | None:intergenic | 15.0% |
| !! | AAATAAGATAAATGAGATAA+GGG | - | chr4_4:82839367-82839386 | None:intergenic | 15.0% |
| !!! | GAAATTCAAACATTTTTTTA+AGG | - | chr4_4:82838738-82838757 | None:intergenic | 15.0% |
| !! | AAATAAGATAAATGAGATAA+GGG | - | chr4_4:82839367-82839386 | None:intergenic | 15.0% |
| !!! | AGTTAAAATCATCTCATTTT+CGG | - | chr4_4:82838605-82838624 | None:intergenic | 20.0% |
| !! | GTTATGAAGAAATAAATATG+AGG | + | chr4_4:82838896-82838915 | Msa0709010:intron | 20.0% |
| !! | CAAATAAGATAAATGAGATA+AGG | - | chr4_4:82839368-82839387 | None:intergenic | 20.0% |
| !!! | ATCTTATTTGAAAGATTGAA+AGG | + | chr4_4:82839378-82839397 | Msa0709010:intron | 20.0% |
| !!! | AGAAACTTTTGAGTAATTTA+CGG | + | chr4_4:82839470-82839489 | Msa0709010:exon | 20.0% |
| !! | AAGTTTGAGTAATTTAAAGA+AGG | + | chr4_4:82839530-82839549 | Msa0709010:three_prime_UTR | 20.0% |
| !!! | TAATTAATGTATGTGACTTT+TGG | + | chr4_4:82839592-82839611 | Msa0709010:three_prime_UTR | 20.0% |
| !!! | AGTTAAAATCATCTCATTTT+CGG | - | chr4_4:82838605-82838624 | None:intergenic | 20.0% |
| !! | GTTATGAAGAAATAAATATG+AGG | + | chr4_4:82838896-82838915 | Msa0709010:intron | 20.0% |
| !! | CAAATAAGATAAATGAGATA+AGG | - | chr4_4:82839368-82839387 | None:intergenic | 20.0% |
| !!! | ATCTTATTTGAAAGATTGAA+AGG | + | chr4_4:82839378-82839397 | Msa0709010:intron | 20.0% |
| !!! | AGAAACTTTTGAGTAATTTA+CGG | + | chr4_4:82839470-82839489 | Msa0709010:exon | 20.0% |
| !! | AAGTTTGAGTAATTTAAAGA+AGG | + | chr4_4:82839530-82839549 | Msa0709010:three_prime_UTR | 20.0% |
| !!! | TAATTAATGTATGTGACTTT+TGG | + | chr4_4:82839592-82839611 | Msa0709010:three_prime_UTR | 20.0% |
| ! | TTTGTTTGTAGATCAATGAA+GGG | - | chr4_4:82838833-82838852 | None:intergenic | 25.0% |
| ! | ATCTGTAGATTTAAAAAAGC+AGG | - | chr4_4:82838985-82839004 | None:intergenic | 25.0% |
| !!! | ATTTAAAGAAGGCATCTTTT+TGG | + | chr4_4:82839541-82839560 | Msa0709010:three_prime_UTR | 25.0% |
| ! | TTTGTTTGTAGATCAATGAA+GGG | - | chr4_4:82838833-82838852 | None:intergenic | 25.0% |
| ! | ATCTGTAGATTTAAAAAAGC+AGG | - | chr4_4:82838985-82839004 | None:intergenic | 25.0% |
| !!! | ATTTAAAGAAGGCATCTTTT+TGG | + | chr4_4:82839541-82839560 | Msa0709010:three_prime_UTR | 25.0% |
| ! | TCTTCAGTTTTTGCAATTTC+AGG | - | chr4_4:82838654-82838673 | None:intergenic | 30.0% |
| ! | CTTTGTTTGTAGATCAATGA+AGG | - | chr4_4:82838834-82838853 | None:intergenic | 30.0% |
| AAAGAAAAGAGACACAAATG+AGG | + | chr4_4:82838850-82838869 | Msa0709010:intron | 30.0% | |
| GAATACAAACAATTGCAGAA+AGG | + | chr4_4:82839417-82839436 | Msa0709010:CDS | 30.0% | |
| ! | TCTTCAGTTTTTGCAATTTC+AGG | - | chr4_4:82838654-82838673 | None:intergenic | 30.0% |
| ! | CTTTGTTTGTAGATCAATGA+AGG | - | chr4_4:82838834-82838853 | None:intergenic | 30.0% |
| AAAGAAAAGAGACACAAATG+AGG | + | chr4_4:82838850-82838869 | Msa0709010:intron | 30.0% | |
| GAATACAAACAATTGCAGAA+AGG | + | chr4_4:82839417-82839436 | Msa0709010:CDS | 30.0% | |
| AGAAATTACTCGTCTTCATC+AGG | + | chr4_4:82838561-82838580 | Msa0709010:five_prime_UTR | 35.0% | |
| GTTGTTGCACGATAATGTAA+TGG | + | chr4_4:82838798-82838817 | Msa0709010:intron | 35.0% | |
| ! | CATTGCTGTAAAACGTGTTT+TGG | - | chr4_4:82839510-82839529 | None:intergenic | 35.0% |
| !! | TGTATGTGACTTTTGGAGAA+TGG | + | chr4_4:82839599-82839618 | Msa0709010:three_prime_UTR | 35.0% |
| AGGCAATCAATACAACTTAG+TGG | - | chr4_4:82839645-82839664 | None:intergenic | 35.0% | |
| AGAAATTACTCGTCTTCATC+AGG | + | chr4_4:82838561-82838580 | Msa0709010:five_prime_UTR | 35.0% | |
| GTTGTTGCACGATAATGTAA+TGG | + | chr4_4:82838798-82838817 | Msa0709010:intron | 35.0% | |
| ! | CATTGCTGTAAAACGTGTTT+TGG | - | chr4_4:82839510-82839529 | None:intergenic | 35.0% |
| !! | TGTATGTGACTTTTGGAGAA+TGG | + | chr4_4:82839599-82839618 | Msa0709010:three_prime_UTR | 35.0% |
| AGGCAATCAATACAACTTAG+TGG | - | chr4_4:82839645-82839664 | None:intergenic | 35.0% | |
| CAATTTCAGGCGACAGTTTA+AGG | - | chr4_4:82838641-82838660 | None:intergenic | 40.0% | |
| ! | TGCAAAAACTGAAGAGTGTG+TGG | + | chr4_4:82838659-82838678 | Msa0709010:CDS | 40.0% |
| ! | GCAAAAACTGAAGAGTGTGT+GGG | + | chr4_4:82838660-82838679 | Msa0709010:CDS | 40.0% |
| TTTATAGAGCAGAGAGACAG+TGG | - | chr4_4:82838777-82838796 | None:intergenic | 40.0% | |
| TTGCCTCAATTATCACGTGA+AGG | + | chr4_4:82839659-82839678 | Msa0709010:three_prime_UTR | 40.0% | |
| CAACCTTCACGTGATAATTG+AGG | - | chr4_4:82839665-82839684 | None:intergenic | 40.0% | |
| CAATTTCAGGCGACAGTTTA+AGG | - | chr4_4:82838641-82838660 | None:intergenic | 40.0% | |
| ! | TGCAAAAACTGAAGAGTGTG+TGG | + | chr4_4:82838659-82838678 | Msa0709010:CDS | 40.0% |
| ! | GCAAAAACTGAAGAGTGTGT+GGG | + | chr4_4:82838660-82838679 | Msa0709010:CDS | 40.0% |
| TTTATAGAGCAGAGAGACAG+TGG | - | chr4_4:82838777-82838796 | None:intergenic | 40.0% | |
| TTGCCTCAATTATCACGTGA+AGG | + | chr4_4:82839659-82839678 | Msa0709010:three_prime_UTR | 40.0% | |
| CAACCTTCACGTGATAATTG+AGG | - | chr4_4:82839665-82839684 | None:intergenic | 40.0% | |
| !! | TTTATATGATAAAAAATATT+TGG | + | chr4_4:82839184-82839203 | Msa0709010:intron | 5.0% |
| !! | TTTATATGATAAAAAATATT+TGG | + | chr4_4:82839184-82839203 | Msa0709010:intron | 5.0% |
| ! | TGAAGAGTGTGTGGGAGAGA+AGG | + | chr4_4:82838668-82838687 | Msa0709010:CDS | 50.0% |
| TAGAGCAGAGAGACAGTGGT+TGG | - | chr4_4:82838773-82838792 | None:intergenic | 50.0% | |
| ! | TGAAGAGTGTGTGGGAGAGA+AGG | + | chr4_4:82838668-82838687 | Msa0709010:CDS | 50.0% |
| TAGAGCAGAGAGACAGTGGT+TGG | - | chr4_4:82838773-82838792 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 82838549 | 82839685 | 82838549 | ID=Msa0709010;Name=Msa0709010 |
| chr4_4 | mRNA | 82838549 | 82839685 | 82838549 | ID=Msa0709010-mRNA-1;Parent=Msa0709010;Name=Msa0709010-mRNA-1;_AED=0.30;_eAED=0.53;_QI=64|0|0.5|1|0|0|2|200|56 |
| chr4_4 | exon | 82838549 | 82838689 | 82838549 | ID=Msa0709010-mRNA-1:exon:27311;Parent=Msa0709010-mRNA-1 |
| chr4_4 | exon | 82839392 | 82839685 | 82839392 | ID=Msa0709010-mRNA-1:exon:27312;Parent=Msa0709010-mRNA-1 |
| chr4_4 | five_prime_UTR | 82838549 | 82838612 | 82838549 | ID=Msa0709010-mRNA-1:five_prime_utr;Parent=Msa0709010-mRNA-1 |
| chr4_4 | CDS | 82838613 | 82838689 | 82838613 | ID=Msa0709010-mRNA-1:cds;Parent=Msa0709010-mRNA-1 |
| chr4_4 | CDS | 82839392 | 82839485 | 82839392 | ID=Msa0709010-mRNA-1:cds;Parent=Msa0709010-mRNA-1 |
| chr4_4 | three_prime_UTR | 82839486 | 82839685 | 82839486 | ID=Msa0709010-mRNA-1:three_prime_utr;Parent=Msa0709010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
