AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0701830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCAGGCGACAGTTTA+AGG 0.122983 4_4:+73288076 None:intergenic
TAATTAATGTATGTGACTTT+TGG 0.258825 4_4:-73287121 Msa0701830:three_prime_UTR
CATTGCTGTAAAACGTGTTT+TGG 0.309510 4_4:+73287206 None:intergenic
TTGAAATTTGAAGAAATAGA+AGG 0.329500 4_4:+73288371 None:intergenic
TAGAAATAGAAGGTAAATGA+TGG 0.424500 4_4:+73288326 None:intergenic
AGAAATTACTCGTCTTCATC+AGG 0.431687 4_4:-73288153 Msa0701830:five_prime_UTR
TTGATTGTTGTTATTGCTGC+AGG 0.431879 4_4:-73288187 Msa0701830:five_prime_UTR
AAGTTTGAGTAATTTAAAGA+AGG 0.477272 4_4:-73287183 Msa0701830:three_prime_UTR
TTGTAGAAAGAATGTAAGAG+AGG 0.519392 4_4:+73288516 None:intergenic
TAGTAATATAGAAGATGAGA+AGG 0.519700 4_4:+73288273 None:intergenic
TGTAGAAAGAATGTAAGAGA+GGG 0.528156 4_4:+73288517 None:intergenic
ATCTTATTTGAAAGATTGAA+AGG 0.529072 4_4:-73287335 Msa0701830:intron
AGGCAATCAATACAACTTAG+TGG 0.556871 4_4:+73287071 None:intergenic
TGAAGAGTGTGTGGGAGAGA+AGG 0.573340 4_4:-73288046 Msa0701830:intron
TTGCCTCAATTATCACGTGA+AGG 0.577241 4_4:-73287054 Msa0701830:three_prime_UTR
CAACCTTCACGTGATAATTG+AGG 0.587359 4_4:+73287051 None:intergenic
GAATACAAACAATTGCAGAA+AGG 0.599398 4_4:-73287296 Msa0701830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTCAAACATTTTTTTA+AGG + chr4_4:73287672-73287691 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr4_4:73288302-73288321 None:intergenic 15.0%
!!! GAAATTCAAACATTTTTTTA+AGG + chr4_4:73287672-73287691 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr4_4:73288302-73288321 None:intergenic 15.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr4_4:73287280-73287299 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr4_4:73287335-73287354 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr4_4:73287539-73287558 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr4_4:73287830-73287849 Msa0701830:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr4_4:73288303-73288322 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr4_4:73288313-73288332 Msa0701830:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr4_4:73288405-73288424 Msa0701830:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr4_4:73288465-73288484 Msa0701830:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr4_4:73288527-73288546 Msa0701830:five_prime_UTR 20.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr4_4:73287280-73287299 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr4_4:73287335-73287354 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr4_4:73287539-73287558 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr4_4:73287830-73287849 Msa0701830:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr4_4:73288303-73288322 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr4_4:73288313-73288332 Msa0701830:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr4_4:73288405-73288424 Msa0701830:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr4_4:73288465-73288484 Msa0701830:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr4_4:73288527-73288546 Msa0701830:five_prime_UTR 20.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr4_4:73287325-73287344 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr4_4:73287378-73287397 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr4_4:73287767-73287786 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr4_4:73287919-73287938 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr4_4:73288476-73288495 Msa0701830:five_prime_UTR 25.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr4_4:73287325-73287344 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr4_4:73287378-73287397 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr4_4:73287767-73287786 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr4_4:73287919-73287938 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr4_4:73288476-73288495 Msa0701830:five_prime_UTR 25.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr4_4:73287134-73287153 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr4_4:73287135-73287154 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr4_4:73287184-73287203 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr4_4:73287588-73287607 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr4_4:73287768-73287787 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr4_4:73287784-73287803 Msa0701830:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr4_4:73288352-73288371 Msa0701830:five_prime_UTR 30.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr4_4:73287134-73287153 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr4_4:73287135-73287154 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr4_4:73287184-73287203 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr4_4:73287588-73287607 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr4_4:73287768-73287787 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr4_4:73287784-73287803 Msa0701830:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr4_4:73288352-73288371 Msa0701830:five_prime_UTR 30.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr4_4:73287461-73287480 Msa0701830:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr4_4:73287495-73287514 Msa0701830:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr4_4:73287720-73287739 Msa0701830:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr4_4:73287732-73287751 Msa0701830:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr4_4:73288445-73288464 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr4_4:73288534-73288553 Msa0701830:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr4_4:73288580-73288599 None:intergenic 35.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr4_4:73287461-73287480 Msa0701830:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr4_4:73287495-73287514 Msa0701830:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr4_4:73287720-73287739 Msa0701830:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr4_4:73287732-73287751 Msa0701830:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr4_4:73288445-73288464 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr4_4:73288534-73288553 Msa0701830:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr4_4:73288580-73288599 None:intergenic 35.0%
CAATTTCAGGCGACAGTTTA+AGG + chr4_4:73287575-73287594 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr4_4:73287593-73287612 Msa0701830:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr4_4:73287594-73287613 Msa0701830:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr4_4:73287711-73287730 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr4_4:73288594-73288613 Msa0701830:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr4_4:73288600-73288619 None:intergenic 40.0%
CAATTTCAGGCGACAGTTTA+AGG + chr4_4:73287575-73287594 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr4_4:73287593-73287612 Msa0701830:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr4_4:73287594-73287613 Msa0701830:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr4_4:73287711-73287730 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr4_4:73288594-73288613 Msa0701830:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr4_4:73288600-73288619 None:intergenic 40.0%
!! TTTATATGATAAAAAATATT+TGG - chr4_4:73288119-73288138 Msa0701830:exon 5.0%
!! TTTATATGATAAAAAATATT+TGG - chr4_4:73288119-73288138 Msa0701830:exon 5.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr4_4:73287602-73287621 Msa0701830:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr4_4:73287707-73287726 None:intergenic 50.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr4_4:73287602-73287621 Msa0701830:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr4_4:73287707-73287726 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 73287044 73288626 73287044 ID=Msa0701830;Name=Msa0701830
chr4_4 mRNA 73287044 73288626 73287044 ID=Msa0701830-mRNA-1;Parent=Msa0701830;Name=Msa0701830-mRNA-1;_AED=0.10;_eAED=0.10;_QI=503|0.5|0.66|1|0|0.33|3|206|58
chr4_4 exon 73287044 73287343 73287044 ID=Msa0701830-mRNA-1:exon:22542;Parent=Msa0701830-mRNA-1
chr4_4 exon 73287923 73287928 73287923 ID=Msa0701830-mRNA-1:exon:22541;Parent=Msa0701830-mRNA-1
chr4_4 exon 73288047 73288626 73288047 ID=Msa0701830-mRNA-1:exon:22540;Parent=Msa0701830-mRNA-1
chr4_4 five_prime_UTR 73288124 73288626 73288124 ID=Msa0701830-mRNA-1:five_prime_utr;Parent=Msa0701830-mRNA-1
chr4_4 CDS 73288047 73288123 73288047 ID=Msa0701830-mRNA-1:cds;Parent=Msa0701830-mRNA-1
chr4_4 CDS 73287923 73287928 73287923 ID=Msa0701830-mRNA-1:cds;Parent=Msa0701830-mRNA-1
chr4_4 CDS 73287250 73287343 73287250 ID=Msa0701830-mRNA-1:cds;Parent=Msa0701830-mRNA-1
chr4_4 three_prime_UTR 73287044 73287249 73287044 ID=Msa0701830-mRNA-1:three_prime_utr;Parent=Msa0701830-mRNA-1
Gene Sequence

>Msa0701830

ATGATTTTAACTGTTAATTTTGTTTCCTTAAACTGTCGCCTGAAATTGCAAAAACTGAAGAGTGTGTGGGAGAGAAGGATAATATTGAAAGGAAGATTGAAAGATGAAGAATACAAACAATTGCAGAAAGGAAGAGAGAAAGAGATGAAGATGTACATAGAAGAAACTTTTGAGTAA

Protein sequence

>Msa0701830

MILTVNFVSLNCRLKLQKLKSVWERRIILKGRLKDEEYKQLQKGREKEMKMYIEETFE*