AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0326940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCAGGCGACAGTTTA+AGG 0.122983 2_4:+38194466 None:intergenic
TAATTAATGTATGTGACTTT+TGG 0.258825 2_4:-38193511 Msa0326940:three_prime_UTR
CATTGCTGTAAAACGTGTTT+TGG 0.309510 2_4:+38193596 None:intergenic
TTGAAATTTGAAGAAATAGA+AGG 0.329500 2_4:+38194761 None:intergenic
TAGAAATAGAAGGTAAATGA+TGG 0.424500 2_4:+38194716 None:intergenic
AGAAATTACTCGTCTTCATC+AGG 0.431687 2_4:-38194543 Msa0326940:five_prime_UTR
TTGATTGTTGTTATTGCTGC+AGG 0.431879 2_4:-38194577 Msa0326940:five_prime_UTR
AAGTTTGAGTAATTTAAAGA+AGG 0.477272 2_4:-38193573 Msa0326940:three_prime_UTR
TTGTAGAAAGAATGTAAGAG+AGG 0.519392 2_4:+38194906 None:intergenic
TAGTAATATAGAAGATGAGA+AGG 0.519700 2_4:+38194663 None:intergenic
TGTAGAAAGAATGTAAGAGA+GGG 0.528156 2_4:+38194907 None:intergenic
ATCTTATTTGAAAGATTGAA+AGG 0.529072 2_4:-38193725 Msa0326940:intron
AGGCAATCAATACAACTTAG+TGG 0.556871 2_4:+38193461 None:intergenic
TGAAGAGTGTGTGGGAGAGA+AGG 0.573340 2_4:-38194436 Msa0326940:intron
TTGCCTCAATTATCACGTGA+AGG 0.577241 2_4:-38193444 Msa0326940:three_prime_UTR
CAACCTTCACGTGATAATTG+AGG 0.587359 2_4:+38193441 None:intergenic
GAATACAAACAATTGCAGAA+AGG 0.599398 2_4:-38193686 Msa0326940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTCAAACATTTTTTTA+AGG + chr2_4:38194062-38194081 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr2_4:38194692-38194711 None:intergenic 15.0%
!!! GAAATTCAAACATTTTTTTA+AGG + chr2_4:38194062-38194081 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr2_4:38194692-38194711 None:intergenic 15.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr2_4:38193670-38193689 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr2_4:38193725-38193744 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr2_4:38193929-38193948 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr2_4:38194220-38194239 Msa0326940:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr2_4:38194693-38194712 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr2_4:38194703-38194722 Msa0326940:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr2_4:38194795-38194814 Msa0326940:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr2_4:38194855-38194874 Msa0326940:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr2_4:38194917-38194936 Msa0326940:five_prime_UTR 20.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr2_4:38193670-38193689 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr2_4:38193725-38193744 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr2_4:38193929-38193948 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr2_4:38194220-38194239 Msa0326940:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr2_4:38194693-38194712 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr2_4:38194703-38194722 Msa0326940:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr2_4:38194795-38194814 Msa0326940:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr2_4:38194855-38194874 Msa0326940:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr2_4:38194917-38194936 Msa0326940:five_prime_UTR 20.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr2_4:38193715-38193734 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr2_4:38193768-38193787 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr2_4:38194157-38194176 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr2_4:38194309-38194328 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr2_4:38194866-38194885 Msa0326940:five_prime_UTR 25.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr2_4:38193715-38193734 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr2_4:38193768-38193787 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr2_4:38194157-38194176 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr2_4:38194309-38194328 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr2_4:38194866-38194885 Msa0326940:five_prime_UTR 25.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr2_4:38193524-38193543 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr2_4:38193525-38193544 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr2_4:38193574-38193593 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr2_4:38193978-38193997 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr2_4:38194158-38194177 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr2_4:38194174-38194193 Msa0326940:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr2_4:38194742-38194761 Msa0326940:five_prime_UTR 30.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr2_4:38193524-38193543 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr2_4:38193525-38193544 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr2_4:38193574-38193593 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr2_4:38193978-38193997 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr2_4:38194158-38194177 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr2_4:38194174-38194193 Msa0326940:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr2_4:38194742-38194761 Msa0326940:five_prime_UTR 30.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr2_4:38193851-38193870 Msa0326940:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr2_4:38193885-38193904 Msa0326940:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr2_4:38194110-38194129 Msa0326940:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr2_4:38194122-38194141 Msa0326940:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr2_4:38194835-38194854 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr2_4:38194924-38194943 Msa0326940:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr2_4:38194970-38194989 None:intergenic 35.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr2_4:38193851-38193870 Msa0326940:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr2_4:38193885-38193904 Msa0326940:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr2_4:38194110-38194129 Msa0326940:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr2_4:38194122-38194141 Msa0326940:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr2_4:38194835-38194854 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr2_4:38194924-38194943 Msa0326940:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr2_4:38194970-38194989 None:intergenic 35.0%
CAATTTCAGGCGACAGTTTA+AGG + chr2_4:38193965-38193984 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr2_4:38193983-38194002 Msa0326940:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr2_4:38193984-38194003 Msa0326940:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr2_4:38194101-38194120 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr2_4:38194984-38195003 Msa0326940:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr2_4:38194990-38195009 None:intergenic 40.0%
CAATTTCAGGCGACAGTTTA+AGG + chr2_4:38193965-38193984 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr2_4:38193983-38194002 Msa0326940:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr2_4:38193984-38194003 Msa0326940:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr2_4:38194101-38194120 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr2_4:38194984-38195003 Msa0326940:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr2_4:38194990-38195009 None:intergenic 40.0%
!! TTTATATGATAAAAAATATT+TGG - chr2_4:38194509-38194528 Msa0326940:exon 5.0%
!! TTTATATGATAAAAAATATT+TGG - chr2_4:38194509-38194528 Msa0326940:exon 5.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr2_4:38193992-38194011 Msa0326940:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr2_4:38194097-38194116 None:intergenic 50.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr2_4:38193992-38194011 Msa0326940:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr2_4:38194097-38194116 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 38193434 38195016 38193434 ID=Msa0326940;Name=Msa0326940
chr2_4 mRNA 38193434 38195016 38193434 ID=Msa0326940-mRNA-1;Parent=Msa0326940;Name=Msa0326940-mRNA-1;_AED=0.10;_eAED=0.10;_QI=503|0|1|1|0|0.5|2|206|56
chr2_4 exon 38193434 38193733 38193434 ID=Msa0326940-mRNA-1:exon:11484;Parent=Msa0326940-mRNA-1
chr2_4 exon 38194437 38195016 38194437 ID=Msa0326940-mRNA-1:exon:11483;Parent=Msa0326940-mRNA-1
chr2_4 five_prime_UTR 38194514 38195016 38194514 ID=Msa0326940-mRNA-1:five_prime_utr;Parent=Msa0326940-mRNA-1
chr2_4 CDS 38194437 38194513 38194437 ID=Msa0326940-mRNA-1:cds;Parent=Msa0326940-mRNA-1
chr2_4 CDS 38193640 38193733 38193640 ID=Msa0326940-mRNA-1:cds;Parent=Msa0326940-mRNA-1
chr2_4 three_prime_UTR 38193434 38193639 38193434 ID=Msa0326940-mRNA-1:three_prime_utr;Parent=Msa0326940-mRNA-1
Gene Sequence

>Msa0326940

ATGATTTTAACTGTTAATTTTGTTTCCTTAAACTGTCGCCTGAAATTGCAAAAACTGAAGAGTGTGTGGGAGAGAAGATTGAAAGGAAGATTGAAAGATGAAGAATACAAACAATTGCAGAAAGGAAGAGAGAAAGAGATGAAGATGTACATAGAAGAAACTTTTGAGTAA

Protein sequence

>Msa0326940

MILTVNFVSLNCRLKLQKLKSVWERRLKGRLKDEEYKQLQKGREKEMKMYIEETFE*