AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0703360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCAGGCGACAGTTTA+AGG 0.122983 4_4:+75249199 None:intergenic
TAATTAATGTATGTGACTTT+TGG 0.258825 4_4:-75248244 Msa0703360:three_prime_UTR
CATTGCTGTAAAACGTGTTT+TGG 0.309510 4_4:+75248329 None:intergenic
TTGAAATTTGAAGAAATAGA+AGG 0.329500 4_4:+75249494 None:intergenic
TAGAAATAGAAGGTAAATGA+TGG 0.424500 4_4:+75249449 None:intergenic
AGAAATTACTCGTCTTCATC+AGG 0.431687 4_4:-75249276 Msa0703360:five_prime_UTR
TTGATTGTTGTTATTGCTGC+AGG 0.431879 4_4:-75249310 Msa0703360:five_prime_UTR
AAGTTTGAGTAATTTAAAGA+AGG 0.477272 4_4:-75248306 Msa0703360:three_prime_UTR
TTGTAGAAAGAATGTAAGAG+AGG 0.519392 4_4:+75249639 None:intergenic
TAGTAATATAGAAGATGAGA+AGG 0.519700 4_4:+75249396 None:intergenic
TGTAGAAAGAATGTAAGAGA+GGG 0.528156 4_4:+75249640 None:intergenic
ATCTTATTTGAAAGATTGAA+AGG 0.529072 4_4:-75248458 Msa0703360:intron
AGGCAATCAATACAACTTAG+TGG 0.556871 4_4:+75248194 None:intergenic
TGAAGAGTGTGTGGGAGAGA+AGG 0.573340 4_4:-75249169 Msa0703360:intron
TTGCCTCAATTATCACGTGA+AGG 0.577241 4_4:-75248177 Msa0703360:three_prime_UTR
CAACCTTCACGTGATAATTG+AGG 0.587359 4_4:+75248174 None:intergenic
GAATACAAACAATTGCAGAA+AGG 0.599398 4_4:-75248419 Msa0703360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTCAAACATTTTTTTA+AGG + chr4_4:75248795-75248814 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr4_4:75249425-75249444 None:intergenic 15.0%
!!! GAAATTCAAACATTTTTTTA+AGG + chr4_4:75248795-75248814 None:intergenic 15.0%
!! AAATAAGATAAATGAGATAA+GGG + chr4_4:75249425-75249444 None:intergenic 15.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr4_4:75248403-75248422 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr4_4:75248458-75248477 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr4_4:75248662-75248681 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr4_4:75248953-75248972 Msa0703360:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr4_4:75249426-75249445 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr4_4:75249436-75249455 Msa0703360:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr4_4:75249528-75249547 Msa0703360:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr4_4:75249588-75249607 Msa0703360:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr4_4:75249650-75249669 Msa0703360:five_prime_UTR 20.0%
!! TTGAAATTTGAAGAAATAGA+AGG + chr4_4:75248403-75248422 None:intergenic 20.0%
!!! TACACATTTTTAGAAATAGA+AGG + chr4_4:75248458-75248477 None:intergenic 20.0%
!!! AGTTAAAATCATCTCATTTT+CGG + chr4_4:75248662-75248681 None:intergenic 20.0%
!! GTTATGAAGAAATAAATATG+AGG - chr4_4:75248953-75248972 Msa0703360:intron 20.0%
!! CAAATAAGATAAATGAGATA+AGG + chr4_4:75249426-75249445 None:intergenic 20.0%
!!! ATCTTATTTGAAAGATTGAA+AGG - chr4_4:75249436-75249455 Msa0703360:five_prime_UTR 20.0%
!!! AGAAACTTTTGAGTAATTTA+CGG - chr4_4:75249528-75249547 Msa0703360:five_prime_UTR 20.0%
!! AAGTTTGAGTAATTTAAAGA+AGG - chr4_4:75249588-75249607 Msa0703360:five_prime_UTR 20.0%
!!! TAATTAATGTATGTGACTTT+TGG - chr4_4:75249650-75249669 Msa0703360:five_prime_UTR 20.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr4_4:75248448-75248467 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr4_4:75248501-75248520 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr4_4:75248890-75248909 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr4_4:75249042-75249061 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr4_4:75249599-75249618 Msa0703360:five_prime_UTR 25.0%
! TAGAAATAGAAGGTAAATGA+TGG + chr4_4:75248448-75248467 None:intergenic 25.0%
! TAGTAATATAGAAGATGAGA+AGG + chr4_4:75248501-75248520 None:intergenic 25.0%
! TTTGTTTGTAGATCAATGAA+GGG + chr4_4:75248890-75248909 None:intergenic 25.0%
! ATCTGTAGATTTAAAAAAGC+AGG + chr4_4:75249042-75249061 None:intergenic 25.0%
!!! ATTTAAAGAAGGCATCTTTT+TGG - chr4_4:75249599-75249618 Msa0703360:five_prime_UTR 25.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr4_4:75248257-75248276 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr4_4:75248258-75248277 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr4_4:75248307-75248326 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr4_4:75248711-75248730 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr4_4:75248891-75248910 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr4_4:75248907-75248926 Msa0703360:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr4_4:75249475-75249494 Msa0703360:five_prime_UTR 30.0%
TGTAGAAAGAATGTAAGAGA+GGG + chr4_4:75248257-75248276 None:intergenic 30.0%
TTGTAGAAAGAATGTAAGAG+AGG + chr4_4:75248258-75248277 None:intergenic 30.0%
!!! GAGTTTTAGCTAAAATGAGA+AGG + chr4_4:75248307-75248326 None:intergenic 30.0%
! TCTTCAGTTTTTGCAATTTC+AGG + chr4_4:75248711-75248730 None:intergenic 30.0%
! CTTTGTTTGTAGATCAATGA+AGG + chr4_4:75248891-75248910 None:intergenic 30.0%
AAAGAAAAGAGACACAAATG+AGG - chr4_4:75248907-75248926 Msa0703360:intron 30.0%
GAATACAAACAATTGCAGAA+AGG - chr4_4:75249475-75249494 Msa0703360:five_prime_UTR 30.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr4_4:75248584-75248603 Msa0703360:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr4_4:75248618-75248637 Msa0703360:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr4_4:75248843-75248862 Msa0703360:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr4_4:75248855-75248874 Msa0703360:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr4_4:75249568-75249587 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr4_4:75249657-75249676 Msa0703360:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr4_4:75249703-75249722 None:intergenic 35.0%
! TTGATTGTTGTTATTGCTGC+AGG - chr4_4:75248584-75248603 Msa0703360:intron 35.0%
AGAAATTACTCGTCTTCATC+AGG - chr4_4:75248618-75248637 Msa0703360:intron 35.0%
TGCTCTATAAATGTTGTTGC+AGG - chr4_4:75248843-75248862 Msa0703360:intron 35.0%
GTTGTTGCAGGATAATGTAA+TGG - chr4_4:75248855-75248874 Msa0703360:intron 35.0%
! CATTGCTGTAAAACGTGTTT+TGG + chr4_4:75249568-75249587 None:intergenic 35.0%
!! TGTATGTGACTTTTGGAGAA+TGG - chr4_4:75249657-75249676 Msa0703360:five_prime_UTR 35.0%
AGGCAATCAATACAACTTAG+TGG + chr4_4:75249703-75249722 None:intergenic 35.0%
CAATTTCAGGCGACAGTTTA+AGG + chr4_4:75248698-75248717 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr4_4:75248716-75248735 Msa0703360:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr4_4:75248717-75248736 Msa0703360:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr4_4:75248834-75248853 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr4_4:75249717-75249736 Msa0703360:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr4_4:75249723-75249742 None:intergenic 40.0%
CAATTTCAGGCGACAGTTTA+AGG + chr4_4:75248698-75248717 None:intergenic 40.0%
! TGCAAAAACTGAAGAGTGTG+TGG - chr4_4:75248716-75248735 Msa0703360:intron 40.0%
! GCAAAAACTGAAGAGTGTGT+GGG - chr4_4:75248717-75248736 Msa0703360:intron 40.0%
TTTATAGAGCAGAGAGACAG+TGG + chr4_4:75248834-75248853 None:intergenic 40.0%
TTGCCTCAATTATCACGTGA+AGG - chr4_4:75249717-75249736 Msa0703360:five_prime_UTR 40.0%
CAACCTTCACGTGATAATTG+AGG + chr4_4:75249723-75249742 None:intergenic 40.0%
!! TTTATATGATAAAAAATATT+TGG - chr4_4:75249242-75249261 Msa0703360:exon 5.0%
!! TTTATATGATAAAAAATATT+TGG - chr4_4:75249242-75249261 Msa0703360:exon 5.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr4_4:75248725-75248744 Msa0703360:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr4_4:75248830-75248849 None:intergenic 50.0%
! TGAAGAGTGTGTGGGAGAGA+AGG - chr4_4:75248725-75248744 Msa0703360:intron 50.0%
TAGAGCAGAGAGACAGTGGT+TGG + chr4_4:75248830-75248849 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 75248167 75249749 75248167 ID=Msa0703360;Name=Msa0703360
chr4_4 mRNA 75248167 75249749 75248167 ID=Msa0703360-mRNA-1;Parent=Msa0703360;Name=Msa0703360-mRNA-1;_AED=0.10;_eAED=0.10;_QI=503|0.5|0.66|1|0|0|3|206|58
chr4_4 exon 75248167 75248466 75248167 ID=Msa0703360-mRNA-1:exon:23576;Parent=Msa0703360-mRNA-1
chr4_4 exon 75249046 75249051 75249046 ID=Msa0703360-mRNA-1:exon:23575;Parent=Msa0703360-mRNA-1
chr4_4 exon 75249170 75249749 75249170 ID=Msa0703360-mRNA-1:exon:23574;Parent=Msa0703360-mRNA-1
chr4_4 five_prime_UTR 75249247 75249749 75249247 ID=Msa0703360-mRNA-1:five_prime_utr;Parent=Msa0703360-mRNA-1
chr4_4 CDS 75249170 75249246 75249170 ID=Msa0703360-mRNA-1:cds;Parent=Msa0703360-mRNA-1
chr4_4 CDS 75249046 75249051 75249046 ID=Msa0703360-mRNA-1:cds;Parent=Msa0703360-mRNA-1
chr4_4 CDS 75248373 75248466 75248373 ID=Msa0703360-mRNA-1:cds;Parent=Msa0703360-mRNA-1
chr4_4 three_prime_UTR 75248167 75248372 75248167 ID=Msa0703360-mRNA-1:three_prime_utr;Parent=Msa0703360-mRNA-1
Gene Sequence

>Msa0703360

ATGATTTTAACTGTTAATTTTGTTTCCTTAAACTGTCGCCTGAAATTGCAAAAACTGAAGAGTGTGTGGGAGAGAAGGATAATATTGAAAGGAAGATTGAAAGATGAAGAATACAAACAATTGCAGAAAGGAAGAGAGAAAGAGATGAAGATGTACATAGAAGAAACTTTTGAGTAA

Protein sequence

>Msa0703360

MILTVNFVSLNCRLKLQKLKSVWERRIILKGRLKDEEYKQLQKGREKEMKMYIEETFE*