AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0734510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0734510 MtrunA17_Chr1g0146881 44.800 125 38 1 34 127 51 175 8.08e-26 100
Msa0734510 MtrunA17_Chr5g0430961 60.000 70 18 2 74 139 19 82 3.68e-19 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTACTCTGGTGCTCTTTC+TGG 0.065981 5_1:-48575605 None:intergenic
TCATTCCAATAAACAAATTT+TGG 0.112658 5_1:-48577526 None:intergenic
TGAAGCATCCTCTTGCTTTC+AGG 0.176242 5_1:-48577205 None:intergenic
GGTTTCCAAAATTTGTTTAT+TGG 0.188832 5_1:+48577521 Msa0734510:three_prime_UTR
CGACGTTGTTAAACAATATT+AGG 0.200638 5_1:+48577884 Msa0734510:three_prime_UTR
AGATACATTATTTATTTAAA+AGG 0.220179 5_1:+48578594 Msa0734510:three_prime_UTR
CCATTGTTCACTGCCTTCTT+TGG 0.224233 5_1:-48578338 None:intergenic
GGTAAAATTATTGTGAGAAT+TGG 0.244178 5_1:+48576940 Msa0734510:exon
ATGCAACAAATCAAAATTCT+TGG 0.251790 5_1:+48576996 Msa0734510:three_prime_UTR
ACTTATTTCTCTTTGAACTA+TGG 0.254865 5_1:+48577401 Msa0734510:three_prime_UTR
TTAATCAAATTATTGCCTTA+TGG 0.257888 5_1:+48578641 Msa0734510:three_prime_UTR
TTTAACAACGTCGCCAATTC+TGG 0.259391 5_1:-48577874 None:intergenic
TGATAGCTCTTTGCTGGTTA+TGG 0.265278 5_1:+48577945 Msa0734510:three_prime_UTR
ACGGACATTTGTTCTAATTC+TGG 0.268719 5_1:+48575660 Msa0734510:CDS
AGCAAAGAGCTATCAGTTAA+AGG 0.286182 5_1:-48577937 None:intergenic
ATGACGAAGACGGCTTCTTT+TGG 0.298196 5_1:+48575859 Msa0734510:CDS
AGGACAAACTCCTGAGGTTT+AGG 0.305105 5_1:-48578430 None:intergenic
AATAATGTATCTACATTGTT+TGG 0.309174 5_1:-48578583 None:intergenic
TTTAACTGATAGCTCTTTGC+TGG 0.325159 5_1:+48577939 Msa0734510:three_prime_UTR
CGTTGATGTTGGTATTGATA+TGG 0.339498 5_1:+48577663 Msa0734510:three_prime_UTR
TAACGATAATCTGACAAATT+AGG 0.349639 5_1:-48577625 None:intergenic
CGACAAACAACGGGATTAAC+CGG 0.350175 5_1:+48575822 Msa0734510:CDS
GCCAAGGAGTGAACTCTATC+AGG 0.356417 5_1:-48576397 None:intergenic
GCTGGTTATGGTTAATTTCA+AGG 0.358981 5_1:+48577957 Msa0734510:three_prime_UTR
TTTCAACATCTGTTATTAGT+TGG 0.363827 5_1:-48578389 None:intergenic
AAGAAGCCGTCTTCGTCATT+AGG 0.365650 5_1:-48575855 None:intergenic
CGTACTCTGGTGCTCTTTCT+GGG 0.365980 5_1:-48575604 None:intergenic
CAGCGTCAATTCTAAAGATA+TGG 0.386517 5_1:-48576964 None:intergenic
GTAGTAATGTTGCTGACATA+AGG 0.387343 5_1:+48578473 Msa0734510:three_prime_UTR
GTCATTAGGTACGTCTGTTC+CGG 0.387858 5_1:-48575841 None:intergenic
TTTGTGCCGGACAATGTTAA+TGG 0.404700 5_1:+48577159 Msa0734510:three_prime_UTR
GAATTGAATGGTTAGGACTT+AGG 0.405260 5_1:+48575772 Msa0734510:intron
CTGGTGCTCTTTCTGGGGAA+TGG 0.407810 5_1:-48575598 None:intergenic
GTGAGGTGTCAAATACCTTA+TGG 0.416873 5_1:-48577456 None:intergenic
TCGGAGTTTCATGTTCGATC+AGG 0.419556 5_1:+48577837 Msa0734510:three_prime_UTR
GATACATTATTTATTTAAAA+GGG 0.428024 5_1:+48578595 Msa0734510:three_prime_UTR
GTAAAATTATTGTGAGAATT+GGG 0.435840 5_1:+48576941 Msa0734510:exon
TTATTAGTTGGAATTAAGTT+CGG 0.437195 5_1:-48578377 None:intergenic
TTGGCTTTGCTAAACGCAAC+GGG 0.440138 5_1:+48576415 Msa0734510:CDS
ACGTCGCCAATTCTGGCTAT+TGG 0.440204 5_1:-48577867 None:intergenic
TCCTGATAGAGTTCACTCCT+TGG 0.452816 5_1:+48576396 Msa0734510:CDS
TAAATATATTATACATGAAA+GGG 0.456255 5_1:-48577806 None:intergenic
CTTGATCCATTAACATTGTC+CGG 0.456743 5_1:-48577165 None:intergenic
GAAACACCAATAGCCAGAAT+TGG 0.463207 5_1:+48577861 Msa0734510:three_prime_UTR
GGGTGGGGTCGACAAACAAC+GGG 0.471193 5_1:+48575813 Msa0734510:CDS
ATAAATATATTATACATGAA+AGG 0.472068 5_1:-48577807 None:intergenic
TGTCCGGCACAAACATGGTT+GGG 0.482898 5_1:-48577149 None:intergenic
GATCATTGGGGCGTGTGGGT+CGG 0.485426 5_1:+48575933 Msa0734510:CDS
TCGTGGTTTCCCAATATTCG+TGG 0.490199 5_1:+48577500 Msa0734510:three_prime_UTR
TAGTAATGTTGCTGACATAA+GGG 0.491980 5_1:+48578474 Msa0734510:three_prime_UTR
TGCCTTTGACACACAAGGGT+GGG 0.502809 5_1:+48575797 Msa0734510:CDS
CGGAGTTTCATGTTCGATCA+GGG 0.503460 5_1:+48577838 Msa0734510:three_prime_UTR
AAGGGCTATTTCATAAATGA+AGG 0.507074 5_1:-48577788 None:intergenic
GCCTTTGACACACAAGGGTG+GGG 0.507799 5_1:+48575798 Msa0734510:CDS
GCAAGGGATTGAATGTACTT+TGG 0.513385 5_1:+48578299 Msa0734510:three_prime_UTR
CTTGGTTCAAGCTATTAGCC+AGG 0.517123 5_1:+48577014 Msa0734510:three_prime_UTR
AATACCAACATCAACGGAAG+TGG 0.519901 5_1:-48577656 None:intergenic
TGAACCACAGAAACTATAAG+AGG 0.523659 5_1:+48577230 Msa0734510:three_prime_UTR
ATTAAGTTCGGCTCATGTGT+TGG 0.524363 5_1:-48578365 None:intergenic
TATAGCTTCTCCTAAACCTC+AGG 0.528518 5_1:+48578420 Msa0734510:three_prime_UTR
GGTGTCAAATACCTTATGGC+AGG 0.529290 5_1:-48577452 None:intergenic
ATCACCTCTTATAGTTTCTG+TGG 0.529669 5_1:-48577234 None:intergenic
TAGTAGATATTGTAAAATCA+AGG 0.535360 5_1:-48578450 None:intergenic
TATCTTTCATGTACGTACTC+TGG 0.544100 5_1:-48575617 None:intergenic
TAATATATTTATTAAAGAGT+CGG 0.546095 5_1:+48577818 Msa0734510:three_prime_UTR
ACCCCACCCTTGTGTGTCAA+AGG 0.547992 5_1:-48575799 None:intergenic
GAATTGAGATCCCTGCCATA+AGG 0.548179 5_1:+48577441 Msa0734510:three_prime_UTR
ATCTCCACTTCCGTTGATGT+TGG 0.549066 5_1:+48577652 Msa0734510:three_prime_UTR
TTGTCCGGCACAAACATGGT+TGG 0.549962 5_1:-48577150 None:intergenic
CTTGGCTTTGCTAAACGCAA+CGG 0.553494 5_1:+48576414 Msa0734510:CDS
AGCTTGTAAAATAGCAGCAA+TGG 0.557695 5_1:+48576372 Msa0734510:CDS
TAGAACAAATGTCCGTCCAT+AGG 0.561149 5_1:-48575653 None:intergenic
AACATTGTCCGGCACAAACA+TGG 0.571085 5_1:-48577154 None:intergenic
AGGGTGGGGTCGACAAACAA+CGG 0.572450 5_1:+48575812 Msa0734510:CDS
GCAATCCTCGATACCACCAC+TGG 0.574989 5_1:-48575904 None:intergenic
AGGATTGCCTTTGACACACA+AGG 0.578167 5_1:+48575792 Msa0734510:CDS
AGTCCCAACCATGTTTGTGC+CGG 0.582912 5_1:+48577146 Msa0734510:three_prime_UTR
TTTACTGCTCAACCTATGGA+CGG 0.593051 5_1:+48575641 Msa0734510:CDS
TGTTTGTGTCAGATATGTTG+TGG 0.598784 5_1:+48577113 Msa0734510:intron
CTTGCTTTCAGGAAAAGCAG+TGG 0.613067 5_1:-48577194 None:intergenic
CCAAAGAAGGCAGTGAACAA+TGG 0.613591 5_1:+48578338 Msa0734510:three_prime_UTR
TATGGATATTGTTGAATGCG+AGG 0.615803 5_1:+48577419 Msa0734510:three_prime_UTR
TTGCCTTTGACACACAAGGG+TGG 0.618428 5_1:+48575796 Msa0734510:CDS
CATATCAATACCAACATCAA+CGG 0.619493 5_1:-48577662 None:intergenic
GTGTCAAATACCTTATGGCA+GGG 0.624744 5_1:-48577451 None:intergenic
CGTTGCGTTTAGCAAAGCCA+AGG 0.625437 5_1:-48576413 None:intergenic
GGAGTTTCATGTTCGATCAG+GGG 0.626050 5_1:+48577839 Msa0734510:three_prime_UTR
GTACTCTGGTGCTCTTTCTG+GGG 0.627433 5_1:-48575603 None:intergenic
GCTTTGCTAAACGCAACGGG+TGG 0.631397 5_1:+48576418 Msa0734510:CDS
TGAGTCCAGTGGTGGTATCG+AGG 0.631616 5_1:+48575899 Msa0734510:CDS
GGTGACAATGAGTCCAGTGG+TGG 0.656389 5_1:+48575891 Msa0734510:CDS
GGATTGCCTTTGACACACAA+GGG 0.671237 5_1:+48575793 Msa0734510:CDS
GACGTACCTAATGACGAAGA+CGG 0.676225 5_1:+48575849 Msa0734510:CDS
GGAGGTGACAATGAGTCCAG+TGG 0.677714 5_1:+48575888 Msa0734510:CDS
AAATCAAGGACAAACTCCTG+AGG 0.701456 5_1:-48578436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTGATTTATTTTTT+CGG + chr5_1:48576489-48576508 Msa0734510:intron 10.0%
!! ATTTAAATTAGAAAGTAATA+TGG - chr5_1:48576868-48576887 None:intergenic 10.0%
!! TAAATATATTATACATGAAA+GGG - chr5_1:48577809-48577828 None:intergenic 10.0%
!! ATAAATATATTATACATGAA+AGG - chr5_1:48577810-48577829 None:intergenic 10.0%
!! TAATATATTTATTAAAGAGT+CGG + chr5_1:48577818-48577837 Msa0734510:three_prime_UTR 10.0%
!! AGATACATTATTTATTTAAA+AGG + chr5_1:48578594-48578613 Msa0734510:three_prime_UTR 10.0%
!! GATACATTATTTATTTAAAA+GGG + chr5_1:48578595-48578614 Msa0734510:three_prime_UTR 10.0%
!!! AGTTTTTGAATGTAATTATA+TGG + chr5_1:48576073-48576092 Msa0734510:intron 15.0%
!!! GTTTTTGAATGTAATTATAT+GGG + chr5_1:48576074-48576093 Msa0734510:intron 15.0%
!! AATGTTCAAAGAATTATAAA+AGG - chr5_1:48576475-48576494 None:intergenic 15.0%
!!! AGATTTTTAAAGAATATTGA+CGG + chr5_1:48576734-48576753 Msa0734510:intron 15.0%
!! TAATTTAAATTTCTATATGC+AGG + chr5_1:48576878-48576897 Msa0734510:intron 15.0%
!!! ATTTTTTTTTCTTTGCTATA+AGG - chr5_1:48578213-48578232 None:intergenic 15.0%
!!! TTTTAATTTTGCTGATTTTG+TGG + chr5_1:48575744-48575763 Msa0734510:intron 20.0%
!! ATAATTTACAAAGAGTGAAA+TGG + chr5_1:48576117-48576136 Msa0734510:intron 20.0%
!! TGAAATGGAAATTTGAATAT+TGG + chr5_1:48576132-48576151 Msa0734510:intron 20.0%
!!! ATGCAATTACTTTATGTTTT+TGG + chr5_1:48576195-48576214 Msa0734510:intron 20.0%
!!! TGCAATTACTTTATGTTTTT+GGG + chr5_1:48576196-48576215 Msa0734510:intron 20.0%
!! TGATTTAGTTAAAATTCAGA+CGG + chr5_1:48576265-48576284 Msa0734510:intron 20.0%
!! AAGTTTGTAATTAAAAAGTG+TGG - chr5_1:48576579-48576598 None:intergenic 20.0%
!! TTTAATTACAAACTTAGTTC+AGG + chr5_1:48576584-48576603 Msa0734510:intron 20.0%
!! GTAAAATTATTGTGAGAATT+GGG + chr5_1:48576941-48576960 Msa0734510:exon 20.0%
!!! CACTTTGTTATCAAATAAAA+TGG - chr5_1:48577289-48577308 None:intergenic 20.0%
!!! TCATTCCAATAAACAAATTT+TGG - chr5_1:48577529-48577548 None:intergenic 20.0%
!! GATATTAAAGTTAATGCTAT+AGG - chr5_1:48577703-48577722 None:intergenic 20.0%
!!! GATGGAATTTTAAATATGTT+TGG - chr5_1:48578191-48578210 None:intergenic 20.0%
!! AATAGTATATTGTAGATGTA+TGG + chr5_1:48578230-48578249 Msa0734510:intron 20.0%
!! TTATTAGTTGGAATTAAGTT+CGG - chr5_1:48578380-48578399 None:intergenic 20.0%
!! TAGTAGATATTGTAAAATCA+AGG - chr5_1:48578453-48578472 None:intergenic 20.0%
!! AATAATGTATCTACATTGTT+TGG - chr5_1:48578586-48578605 None:intergenic 20.0%
!! TTAATCAAATTATTGCCTTA+TGG + chr5_1:48578641-48578660 Msa0734510:three_prime_UTR 20.0%
! TTGAATGTAATTATATGGGT+TGG + chr5_1:48576078-48576097 Msa0734510:intron 25.0%
! AATATTGGTGAATGATTGTT+GGG + chr5_1:48576147-48576166 Msa0734510:intron 25.0%
! TTAACTACATTAATGATTGC+TGG + chr5_1:48576553-48576572 Msa0734510:intron 25.0%
! GTTGAGAATTTATCTTTCTA+TGG + chr5_1:48576649-48576668 Msa0734510:intron 25.0%
! GGTAAAATTATTGTGAGAAT+TGG + chr5_1:48576940-48576959 Msa0734510:exon 25.0%
! ATGCAACAAATCAAAATTCT+TGG + chr5_1:48576996-48577015 Msa0734510:three_prime_UTR 25.0%
! TGCTAATCTACTAAAATACA+TGG - chr5_1:48577331-48577350 None:intergenic 25.0%
!! ACTTATTTCTCTTTGAACTA+TGG + chr5_1:48577401-48577420 Msa0734510:three_prime_UTR 25.0%
!! GGTTTCCAAAATTTGTTTAT+TGG + chr5_1:48577521-48577540 Msa0734510:three_prime_UTR 25.0%
!!! ATTTTTGTCTGAGACAAAAT+CGG + chr5_1:48577589-48577608 Msa0734510:three_prime_UTR 25.0%
! TAACGATAATCTGACAAATT+AGG - chr5_1:48577628-48577647 None:intergenic 25.0%
! TTATGGTTAATTTCAAGGTA+AGG + chr5_1:48577962-48577981 Msa0734510:intron 25.0%
!! TAACATAAATTAGTTTGACG+AGG - chr5_1:48578032-48578051 None:intergenic 25.0%
! ATACATATCGAAACTTTAGT+CGG + chr5_1:48578054-48578073 Msa0734510:intron 25.0%
!!! TTTTTTCTTTGCTATAAGGA+TGG - chr5_1:48578209-48578228 None:intergenic 25.0%
! TGTATGGTAAGATTTGATAA+AGG + chr5_1:48578246-48578265 Msa0734510:intron 25.0%
! TAAAGGAATTGAATGTATTC+TGG + chr5_1:48578263-48578282 Msa0734510:intron 25.0%
! TTTCAACATCTGTTATTAGT+TGG - chr5_1:48578392-48578411 None:intergenic 25.0%
! ATATTTTACTGCTCAACCTA+TGG + chr5_1:48575637-48575656 Msa0734510:CDS 30.0%
TTTGTGGAGATTGAATTGAA+TGG + chr5_1:48575760-48575779 Msa0734510:intron 30.0%
GAATATTGGTGAATGATTGT+TGG + chr5_1:48576146-48576165 Msa0734510:intron 30.0%
CTCAACCTAATCAAATCTTA+GGG - chr5_1:48576295-48576314 None:intergenic 30.0%
ACTCAACCTAATCAAATCTT+AGG - chr5_1:48576296-48576315 None:intergenic 30.0%
TATTGTTTGCCTATTGAAAC+AGG + chr5_1:48576340-48576359 Msa0734510:intron 30.0%
ATCATAGATCCTGTTTCAAT+AGG - chr5_1:48576352-48576371 None:intergenic 30.0%
! AGATTTTCAATGTTTTCCAG+AGG + chr5_1:48576441-48576460 Msa0734510:CDS 30.0%
! ATAAAAGGTACATATACCTC+TGG - chr5_1:48576460-48576479 None:intergenic 30.0%
CTCAACTAAATTTGTCCTAA+AGG - chr5_1:48576635-48576654 None:intergenic 30.0%
TATTTCTCAAGACAGTTATC+TGG + chr5_1:48576796-48576815 Msa0734510:intron 30.0%
! ACTATAAGAGGTGATGATTT+TGG + chr5_1:48577242-48577261 Msa0734510:intron 30.0%
!! TTTTGGAAACCACGAATATT+GGG - chr5_1:48577512-48577531 None:intergenic 30.0%
!! ATTTTGGAAACCACGAATAT+TGG - chr5_1:48577513-48577532 None:intergenic 30.0%
CATATCAATACCAACATCAA+CGG - chr5_1:48577665-48577684 None:intergenic 30.0%
ATGTATCCATGAAAATATGC+AGG + chr5_1:48577758-48577777 Msa0734510:intron 30.0%
! AAGGGCTATTTCATAAATGA+AGG - chr5_1:48577791-48577810 None:intergenic 30.0%
CGACGTTGTTAAACAATATT+AGG + chr5_1:48577884-48577903 Msa0734510:three_prime_UTR 30.0%
GGTGAAAAAATGATATGTGA+AGG - chr5_1:48578170-48578189 None:intergenic 30.0%
!! TCTTTGGAGATTGATTTTTC+AGG - chr5_1:48578325-48578344 None:intergenic 30.0%
GAAAAATCAATCTCCAAAGA+AGG + chr5_1:48578325-48578344 Msa0734510:three_prime_UTR 30.0%
TAGTAATGTTGCTGACATAA+GGG + chr5_1:48578474-48578493 Msa0734510:three_prime_UTR 30.0%
AAAAACTACACTAGAGGAAT+GGG - chr5_1:48578521-48578540 None:intergenic 30.0%
AAAAAACTACACTAGAGGAA+TGG - chr5_1:48578522-48578541 None:intergenic 30.0%
! TAATCAGACATTTTGTGTCT+TGG + chr5_1:48578555-48578574 Msa0734510:three_prime_UTR 30.0%
!!! TACTGATTTTAGCTTCCATA+AGG - chr5_1:48578659-48578678 None:intergenic 30.0%
TATCTTTCATGTACGTACTC+TGG - chr5_1:48575620-48575639 None:intergenic 35.0%
ACGGACATTTGTTCTAATTC+TGG + chr5_1:48575660-48575679 Msa0734510:CDS 35.0%
GGAGATTGAATTGAATGGTT+AGG + chr5_1:48575765-48575784 Msa0734510:intron 35.0%
GAATTGAATGGTTAGGACTT+AGG + chr5_1:48575772-48575791 Msa0734510:intron 35.0%
!! AGGATTGCGATTTTGATCAT+TGG + chr5_1:48575919-48575938 Msa0734510:CDS 35.0%
!! GGATTGCGATTTTGATCATT+GGG + chr5_1:48575920-48575939 Msa0734510:CDS 35.0%
!! GATTGCGATTTTGATCATTG+GGG + chr5_1:48575921-48575940 Msa0734510:CDS 35.0%
TCTATGCACAATAGAAAGTC+TGG + chr5_1:48575959-48575978 Msa0734510:intron 35.0%
AATAGAAAGTCTGGTCATCA+AGG + chr5_1:48575968-48575987 Msa0734510:intron 35.0%
GTTTGCCCTAAGATTTGATT+AGG + chr5_1:48576287-48576306 Msa0734510:intron 35.0%
AGCTTGTAAAATAGCAGCAA+TGG + chr5_1:48576372-48576391 Msa0734510:CDS 35.0%
!! CTGATTTTAGGTGATCCTTT+AGG + chr5_1:48576617-48576636 Msa0734510:intron 35.0%
TTGAGAAATACTTCACAGGT+TGG - chr5_1:48576786-48576805 None:intergenic 35.0%
TGTCTTGAGAAATACTTCAC+AGG - chr5_1:48576790-48576809 None:intergenic 35.0%
TATGGTGATAAGAAGATGCA+GGG - chr5_1:48576850-48576869 None:intergenic 35.0%
ATATGGTGATAAGAAGATGC+AGG - chr5_1:48576851-48576870 None:intergenic 35.0%
! TTGCTTTCTCAGTTTTTGTG+TGG + chr5_1:48576907-48576926 Msa0734510:CDS 35.0%
! GTTTTTGTGTGGAATGTCTA+CGG + chr5_1:48576918-48576937 Msa0734510:CDS 35.0%
! TTTTTGTGTGGAATGTCTAC+GGG + chr5_1:48576919-48576938 Msa0734510:CDS 35.0%
! CAGCGTCAATTCTAAAGATA+TGG - chr5_1:48576967-48576986 None:intergenic 35.0%
TGTGTATACAGCTCTTCATT+AGG + chr5_1:48577037-48577056 Msa0734510:intron 35.0%
TATACAGCTCTTCATTAGGA+AGG + chr5_1:48577041-48577060 Msa0734510:intron 35.0%
TGTTTGTGTCAGATATGTTG+TGG + chr5_1:48577113-48577132 Msa0734510:intron 35.0%
CTTGATCCATTAACATTGTC+CGG - chr5_1:48577168-48577187 None:intergenic 35.0%
TGAACCACAGAAACTATAAG+AGG + chr5_1:48577230-48577249 Msa0734510:three_prime_UTR 35.0%
ATCACCTCTTATAGTTTCTG+TGG - chr5_1:48577237-48577256 None:intergenic 35.0%
TATGGATATTGTTGAATGCG+AGG + chr5_1:48577419-48577438 Msa0734510:three_prime_UTR 35.0%
CCACGAATATTCAAAAACTC+AGG - chr5_1:48577486-48577505 None:intergenic 35.0%
! CCTGAGTTTTTGAATATTCG+TGG + chr5_1:48577483-48577502 Msa0734510:three_prime_UTR 35.0%
!! CGTTGATGTTGGTATTGATA+TGG + chr5_1:48577663-48577682 Msa0734510:three_prime_UTR 35.0%
AGCAAAGAGCTATCAGTTAA+AGG - chr5_1:48577940-48577959 None:intergenic 35.0%
TTTAACTGATAGCTCTTTGC+TGG + chr5_1:48577939-48577958 Msa0734510:three_prime_UTR 35.0%
GCTGGTTATGGTTAATTTCA+AGG + chr5_1:48577957-48577976 Msa0734510:three_prime_UTR 35.0%
GTATTCTGGTCATACATAAC+AGG + chr5_1:48578277-48578296 Msa0734510:intron 35.0%
GTAGTAATGTTGCTGACATA+AGG + chr5_1:48578473-48578492 Msa0734510:three_prime_UTR 35.0%
CCAACAAAAAACTACACTAG+AGG - chr5_1:48578527-48578546 None:intergenic 35.0%
!!! CCTCTAGTGTAGTTTTTTGT+TGG + chr5_1:48578524-48578543 Msa0734510:three_prime_UTR 35.0%
TTTACTGCTCAACCTATGGA+CGG + chr5_1:48575641-48575660 Msa0734510:CDS 40.0%
TAGAACAAATGTCCGTCCAT+AGG - chr5_1:48575656-48575675 None:intergenic 40.0%
CAGAACAATGATCATGGCAA+AGG + chr5_1:48576172-48576191 Msa0734510:intron 40.0%
TCGTAGCACTAAAATCGTCT+TGG + chr5_1:48576699-48576718 Msa0734510:intron 40.0%
TTTGTGCCGGACAATGTTAA+TGG + chr5_1:48577159-48577178 Msa0734510:three_prime_UTR 40.0%
CATCTCTTGTGAAACTGAAC+AGG + chr5_1:48577374-48577393 Msa0734510:intron 40.0%
GTGTCAAATACCTTATGGCA+GGG - chr5_1:48577454-48577473 None:intergenic 40.0%
! GTGAGGTGTCAAATACCTTA+TGG - chr5_1:48577459-48577478 None:intergenic 40.0%
TCAAAAACTCAGGTGAAGTG+AGG - chr5_1:48577476-48577495 None:intergenic 40.0%
AATACCAACATCAACGGAAG+TGG - chr5_1:48577659-48577678 None:intergenic 40.0%
! CCATCACCTGCATATTTTCA+TGG - chr5_1:48577767-48577786 None:intergenic 40.0%
CCATGAAAATATGCAGGTGA+TGG + chr5_1:48577764-48577783 Msa0734510:intron 40.0%
GAAACACCAATAGCCAGAAT+TGG + chr5_1:48577861-48577880 Msa0734510:three_prime_UTR 40.0%
TTTAACAACGTCGCCAATTC+TGG - chr5_1:48577877-48577896 None:intergenic 40.0%
! TGATAGCTCTTTGCTGGTTA+TGG + chr5_1:48577945-48577964 Msa0734510:three_prime_UTR 40.0%
TGGTCATACATAACAGGCAA+GGG + chr5_1:48578283-48578302 Msa0734510:intron 40.0%
GCAAGGGATTGAATGTACTT+TGG + chr5_1:48578299-48578318 Msa0734510:three_prime_UTR 40.0%
ATTAAGTTCGGCTCATGTGT+TGG - chr5_1:48578368-48578387 None:intergenic 40.0%
TATAGCTTCTCCTAAACCTC+AGG + chr5_1:48578420-48578439 Msa0734510:three_prime_UTR 40.0%
AAATCAAGGACAAACTCCTG+AGG - chr5_1:48578439-48578458 None:intergenic 40.0%
AGGATTGCCTTTGACACACA+AGG + chr5_1:48575792-48575811 Msa0734510:CDS 45.0%
GGATTGCCTTTGACACACAA+GGG + chr5_1:48575793-48575812 Msa0734510:CDS 45.0%
CGACAAACAACGGGATTAAC+CGG + chr5_1:48575822-48575841 Msa0734510:CDS 45.0%
GTCATTAGGTACGTCTGTTC+CGG - chr5_1:48575844-48575863 None:intergenic 45.0%
GACGTACCTAATGACGAAGA+CGG + chr5_1:48575849-48575868 Msa0734510:CDS 45.0%
AAGAAGCCGTCTTCGTCATT+AGG - chr5_1:48575858-48575877 None:intergenic 45.0%
! ATGACGAAGACGGCTTCTTT+TGG + chr5_1:48575859-48575878 Msa0734510:CDS 45.0%
!! ATTTTGATCATTGGGGCGTG+TGG + chr5_1:48575928-48575947 Msa0734510:CDS 45.0%
! TTTTGATCATTGGGGCGTGT+GGG + chr5_1:48575929-48575948 Msa0734510:CDS 45.0%
TGGGTGCAGAACAATGATCA+TGG + chr5_1:48576166-48576185 Msa0734510:intron 45.0%
TCCTGATAGAGTTCACTCCT+TGG + chr5_1:48576396-48576415 Msa0734510:CDS 45.0%
!! CTTGGCTTTGCTAAACGCAA+CGG + chr5_1:48576414-48576433 Msa0734510:CDS 45.0%
!! TTGGCTTTGCTAAACGCAAC+GGG + chr5_1:48576415-48576434 Msa0734510:CDS 45.0%
CAAACTTAGTTCAGGACGAG+TGG + chr5_1:48576592-48576611 Msa0734510:intron 45.0%
!!! GGACGAGTGGTACTGATTTT+AGG + chr5_1:48576605-48576624 Msa0734510:intron 45.0%
CTTGGTTCAAGCTATTAGCC+AGG + chr5_1:48577014-48577033 Msa0734510:three_prime_UTR 45.0%
TGAAGAGCTGTATACACACC+TGG - chr5_1:48577035-48577054 None:intergenic 45.0%
AACATTGTCCGGCACAAACA+TGG - chr5_1:48577157-48577176 None:intergenic 45.0%
CTTGCTTTCAGGAAAAGCAG+TGG - chr5_1:48577197-48577216 None:intergenic 45.0%
!! CTGCTTTTCCTGAAAGCAAG+AGG + chr5_1:48577197-48577216 Msa0734510:three_prime_UTR 45.0%
! TGAAGCATCCTCTTGCTTTC+AGG - chr5_1:48577208-48577227 None:intergenic 45.0%
GAATTGAGATCCCTGCCATA+AGG + chr5_1:48577441-48577460 Msa0734510:three_prime_UTR 45.0%
GGTGTCAAATACCTTATGGC+AGG - chr5_1:48577455-48577474 None:intergenic 45.0%
TCGTGGTTTCCCAATATTCG+TGG + chr5_1:48577500-48577519 Msa0734510:three_prime_UTR 45.0%
ATCTCCACTTCCGTTGATGT+TGG + chr5_1:48577652-48577671 Msa0734510:three_prime_UTR 45.0%
TCGGAGTTTCATGTTCGATC+AGG + chr5_1:48577837-48577856 Msa0734510:three_prime_UTR 45.0%
CGGAGTTTCATGTTCGATCA+GGG + chr5_1:48577838-48577857 Msa0734510:three_prime_UTR 45.0%
GGAGTTTCATGTTCGATCAG+GGG + chr5_1:48577839-48577858 Msa0734510:three_prime_UTR 45.0%
ATACTCTTTCCACCTCCTTG+TGG + chr5_1:48578108-48578127 Msa0734510:intron 45.0%
GAAAACCAACTCCAACCACA+AGG - chr5_1:48578126-48578145 None:intergenic 45.0%
!!! TGTGGTTGGAGTTGGTTTTC+AGG + chr5_1:48578126-48578145 Msa0734510:intron 45.0%
CTGGTCATACATAACAGGCA+AGG + chr5_1:48578282-48578301 Msa0734510:intron 45.0%
CCATTGTTCACTGCCTTCTT+TGG - chr5_1:48578341-48578360 None:intergenic 45.0%
CCAAAGAAGGCAGTGAACAA+TGG + chr5_1:48578338-48578357 Msa0734510:three_prime_UTR 45.0%
AGGACAAACTCCTGAGGTTT+AGG - chr5_1:48578433-48578452 None:intergenic 45.0%
GTACTCTGGTGCTCTTTCTG+GGG - chr5_1:48575606-48575625 None:intergenic 50.0%
CGTACTCTGGTGCTCTTTCT+GGG - chr5_1:48575607-48575626 None:intergenic 50.0%
ACGTACTCTGGTGCTCTTTC+TGG - chr5_1:48575608-48575627 None:intergenic 50.0%
TTGCCTTTGACACACAAGGG+TGG + chr5_1:48575796-48575815 Msa0734510:CDS 50.0%
TGCCTTTGACACACAAGGGT+GGG + chr5_1:48575797-48575816 Msa0734510:CDS 50.0%
!! GAAGACGGCTTCTTTTGGAG+AGG + chr5_1:48575864-48575883 Msa0734510:CDS 50.0%
GCCAAGGAGTGAACTCTATC+AGG - chr5_1:48576400-48576419 None:intergenic 50.0%
CGTTGCGTTTAGCAAAGCCA+AGG - chr5_1:48576416-48576435 None:intergenic 50.0%
AGTCCCAACCATGTTTGTGC+CGG + chr5_1:48577146-48577165 Msa0734510:three_prime_UTR 50.0%
TGTCCGGCACAAACATGGTT+GGG - chr5_1:48577152-48577171 None:intergenic 50.0%
TTGTCCGGCACAAACATGGT+TGG - chr5_1:48577153-48577172 None:intergenic 50.0%
! ACGTCGCCAATTCTGGCTAT+TGG - chr5_1:48577870-48577889 None:intergenic 50.0%
TCTTTCCACCTCCTTGTGGT+TGG + chr5_1:48578112-48578131 Msa0734510:intron 50.0%
AACCAACTCCAACCACAAGG+AGG - chr5_1:48578123-48578142 None:intergenic 50.0%
! CTGGTGCTCTTTCTGGGGAA+TGG - chr5_1:48575601-48575620 None:intergenic 55.0%
GCCTTTGACACACAAGGGTG+GGG + chr5_1:48575798-48575817 Msa0734510:CDS 55.0%
ACCCCACCCTTGTGTGTCAA+AGG - chr5_1:48575802-48575821 None:intergenic 55.0%
AGGGTGGGGTCGACAAACAA+CGG + chr5_1:48575812-48575831 Msa0734510:CDS 55.0%
!! GACGGCTTCTTTTGGAGAGG+AGG + chr5_1:48575867-48575886 Msa0734510:CDS 55.0%
!! GGCTTCTTTTGGAGAGGAGG+AGG + chr5_1:48575870-48575889 Msa0734510:CDS 55.0%
GGAGGTGACAATGAGTCCAG+TGG + chr5_1:48575888-48575907 Msa0734510:CDS 55.0%
GGTGACAATGAGTCCAGTGG+TGG + chr5_1:48575891-48575910 Msa0734510:CDS 55.0%
TGAGTCCAGTGGTGGTATCG+AGG + chr5_1:48575899-48575918 Msa0734510:CDS 55.0%
GCAATCCTCGATACCACCAC+TGG - chr5_1:48575907-48575926 None:intergenic 55.0%
GCTTTGCTAAACGCAACGGG+TGG + chr5_1:48576418-48576437 Msa0734510:CDS 55.0%
CAACTCCAACCACAAGGAGG+TGG - chr5_1:48578120-48578139 None:intergenic 55.0%
CACCTCCTTGTGGTTGGAGT+TGG + chr5_1:48578118-48578137 Msa0734510:intron 55.0%
GGGTGGGGTCGACAAACAAC+GGG + chr5_1:48575813-48575832 Msa0734510:CDS 60.0%
GATCATTGGGGCGTGTGGGT+CGG + chr5_1:48575933-48575952 Msa0734510:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 48575591 48578719 48575591 ID=Msa0734510;Name=Msa0734510
chr5_1 mRNA 48575591 48578719 48575591 ID=Msa0734510-mRNA-1;Parent=Msa0734510;Name=Msa0734510-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0.57|0.75|0.87|0.42|0.37|8|1127|141
chr5_1 exon 48575591 48575681 48575591 ID=Msa0734510-mRNA-1:exon:13878;Parent=Msa0734510-mRNA-1
chr5_1 exon 48575787 48575975 48575787 ID=Msa0734510-mRNA-1:exon:13879;Parent=Msa0734510-mRNA-1
chr5_1 exon 48576362 48576462 48576362 ID=Msa0734510-mRNA-1:exon:13880;Parent=Msa0734510-mRNA-1
chr5_1 exon 48576900 48577035 48576900 ID=Msa0734510-mRNA-1:exon:13881;Parent=Msa0734510-mRNA-1
chr5_1 exon 48577125 48577251 48577125 ID=Msa0734510-mRNA-1:exon:13882;Parent=Msa0734510-mRNA-1
chr5_1 exon 48577396 48577684 48577396 ID=Msa0734510-mRNA-1:exon:13883;Parent=Msa0734510-mRNA-1
chr5_1 exon 48577780 48577978 48577780 ID=Msa0734510-mRNA-1:exon:13884;Parent=Msa0734510-mRNA-1
chr5_1 exon 48578299 48578719 48578299 ID=Msa0734510-mRNA-1:exon:13885;Parent=Msa0734510-mRNA-1
chr5_1 CDS 48575591 48575681 48575591 ID=Msa0734510-mRNA-1:cds;Parent=Msa0734510-mRNA-1
chr5_1 CDS 48575787 48575975 48575787 ID=Msa0734510-mRNA-1:cds;Parent=Msa0734510-mRNA-1
chr5_1 CDS 48576362 48576462 48576362 ID=Msa0734510-mRNA-1:cds;Parent=Msa0734510-mRNA-1
chr5_1 CDS 48576900 48576944 48576900 ID=Msa0734510-mRNA-1:cds;Parent=Msa0734510-mRNA-1
chr5_1 three_prime_UTR 48576945 48577035 48576945 ID=Msa0734510-mRNA-1:three_prime_utr;Parent=Msa0734510-mRNA-1
chr5_1 three_prime_UTR 48577125 48577251 48577125 ID=Msa0734510-mRNA-1:three_prime_utr;Parent=Msa0734510-mRNA-1
chr5_1 three_prime_UTR 48577396 48577684 48577396 ID=Msa0734510-mRNA-1:three_prime_utr;Parent=Msa0734510-mRNA-1
chr5_1 three_prime_UTR 48577780 48577978 48577780 ID=Msa0734510-mRNA-1:three_prime_utr;Parent=Msa0734510-mRNA-1
chr5_1 three_prime_UTR 48578299 48578719 48578299 ID=Msa0734510-mRNA-1:three_prime_utr;Parent=Msa0734510-mRNA-1
Gene Sequence

>Msa0734510

ATGGAATCCATTCCCCAGAAAGAGCACCAGAGTACGTACATGAAAGATATTTTACTGCTCAACCTATGGACGGACATTTGTTCTAATTCTGGACTTAGGATTGCCTTTGACACACAAGGGTGGGGTCGACAAACAACGGGATTAACCGGAACAGACGTACCTAATGACGAAGACGGCTTCTTTTGGAGAGGAGGAGGTGACAATGAGTCCAGTGGTGGTATCGAGGATTGCGATTTTGATCATTGGGGCGTGTGGGTCGGAGCTCTATGCACAATAGAAAGATCTATGATAGCTTGTAAAATAGCAGCAATGGTTCCTGATAGAGTTCACTCCTTGGCTTTGCTAAACGCAACGGGTGGAGATTTTCAATGTTTTCCAGAGGAATCACTTGCTTTCTCAGTTTTTGTGTGGAATGTCTACGGGTAA
Protein sequence

>Msa0734510

MESIPQKEHQSTYMKDILLLNLWTDICSNSGLRIAFDTQGWGRQTTGLTGTDVPNDEDGFFWRGGGDNESSGGIEDCDFDHWGVWVGALCTIERSMIACKIAAMVPDRVHSLALLNATGGDFQCFPEESLAFSVFVWNVYG*