AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0759860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759860 MtrunA17_Chr5g0408121 97.744 133 3 0 1 133 1 133 2.62e-93 265
Msa0759860 MtrunA17_Chr5g0408111 37.662 77 48 0 26 102 54 130 1.11e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGCCGTCGCCGAAATTA+TGG 0.132420 5_2:-15751604 Msa0759860:CDS
TCATCAAACTCTACAGTTTC+CGG 0.200149 5_2:-15751479 Msa0759860:CDS
CACTTAGTGCCCGTTTGTTT+TGG 0.240486 5_2:+15751731 None:intergenic
CATAATTTCGGCGACGGCTT+TGG 0.257033 5_2:+15751605 None:intergenic
ATGAATCCGGCGGGTTTAAC+AGG 0.280966 5_2:-15751651 Msa0759860:CDS
GGAAATGATGGAAGCAATTT+CGG 0.295914 5_2:+15751506 None:intergenic
TTTGAGTATTGAAACTAGTT+TGG 0.342271 5_2:-15750262 Msa0759860:three_prime_UTR
ATCTAACTTGGTTCCTATCT+AGG 0.344574 5_2:+15750365 None:intergenic
CGCCGGATTCATCCTTCTGC+TGG 0.358107 5_2:+15751662 None:intergenic
CAGTGAACTCTGCACTTTCA+TGG 0.368977 5_2:-15751550 Msa0759860:CDS
CTGCATGATAATTTCATGAA+TGG 0.375196 5_2:-15750305 Msa0759860:three_prime_UTR
CATCAAACTCTACAGTTTCC+GGG 0.376717 5_2:-15751478 Msa0759860:intron
ATCGCACCCTGGCTGAATTC+CGG 0.377231 5_2:+15750504 None:intergenic
TTTGCCGACACCCATAATTT+CGG 0.382001 5_2:+15751593 None:intergenic
TGTCCCGTTTGAAACTAGTT+TGG 0.386826 5_2:-15750193 Msa0759860:three_prime_UTR
TTTGATGAACTTGGAAATGA+TGG 0.402949 5_2:+15751494 None:intergenic
AAAGCCGTCGCCGAAATTAT+GGG 0.406307 5_2:-15751603 Msa0759860:CDS
GTATTAAGAAAGACAAGCTT+TGG 0.408648 5_2:-15750599 Msa0759860:CDS
ACTGGTAGCTTTGCGCGTCT+TGG 0.418961 5_2:+15751569 None:intergenic
CAGAAAATGAATATTGTCAT+TGG 0.428328 5_2:+15750224 None:intergenic
ACTGTAGAGTTTGATGAACT+TGG 0.446589 5_2:+15751485 None:intergenic
GGGAAAGGCCAACAGAAGAA+AGG 0.464992 5_2:-15750435 Msa0759860:CDS
CAAAACAAACGGGCACTAAG+TGG 0.465354 5_2:-15751730 Msa0759860:five_prime_UTR
GGATACTAATACGGATTCTT+CGG 0.469159 5_2:-15750472 Msa0759860:CDS
TTTGTTAGTCCAAAACAAAC+GGG 0.474481 5_2:-15751740 Msa0759860:five_prime_UTR
TAATATCAAGGATACTAATA+CGG 0.503603 5_2:-15750481 Msa0759860:CDS
GACAGAATCTTAGCAATCTC+GGG 0.503935 5_2:+15750528 None:intergenic
TCTGTCTCCGGAATTCAGCC+AGG 0.504471 5_2:-15750511 Msa0759860:CDS
GGAAAGGCCAACAGAAGAAA+GGG 0.508550 5_2:-15750434 Msa0759860:CDS
GATTGCTAAGATTCTGTCTC+CGG 0.511126 5_2:-15750523 Msa0759860:CDS
ATAGCCAAACTAGTTTCAAA+CGG 0.511208 5_2:+15750189 None:intergenic
AGTGAACTCTGCACTTTCAT+GGG 0.512210 5_2:-15751549 Msa0759860:CDS
TAGCCAAACTAGTTTCAAAC+GGG 0.513809 5_2:+15750190 None:intergenic
TATGGTGTTGTGCAGAGTCC+TGG 0.516143 5_2:-15750621 Msa0759860:intron
CCAGGGTGCGATTAATATCA+AGG 0.519238 5_2:-15750493 Msa0759860:CDS
TATTAAGAAAGACAAGCTTT+GGG 0.519290 5_2:-15750598 Msa0759860:CDS
AGCTTGTCTTTCTTAATACC+AGG 0.519823 5_2:+15750603 None:intergenic
AGACAGAATCTTAGCAATCT+CGG 0.521268 5_2:+15750527 None:intergenic
CATGAAAGTGCAGAGTTCAC+TGG 0.524418 5_2:+15751551 None:intergenic
ATCCAGCAGAAGGATGAATC+CGG 0.526821 5_2:-15751664 Msa0759860:exon
CTTCGCCCTGTTAAACCCGC+CGG 0.548257 5_2:+15751645 None:intergenic
GACACCCATAATTTCGGCGA+CGG 0.548577 5_2:+15751599 None:intergenic
TCTTCGGCGGATAACACAAA+GGG 0.549211 5_2:-15750456 Msa0759860:CDS
AGGAAGTGGAGGCATGTGGT+TGG 0.560814 5_2:-15750401 Msa0759860:three_prime_UTR
GCGGATAACACAAAGGGGAA+AGG 0.574554 5_2:-15750450 Msa0759860:CDS
GTCGCCGAAATTATGGGTGT+CGG 0.593807 5_2:-15751597 Msa0759860:CDS
CTAGTTTGGCTATTGCCTTG+TGG 0.596708 5_2:-15750248 Msa0759860:three_prime_UTR
AAAACAAACGGGCACTAAGT+GGG 0.598653 5_2:-15751729 Msa0759860:five_prime_UTR
CGGCGGGTTTAACAGGGCGA+AGG 0.602549 5_2:-15751644 Msa0759860:CDS
CGGGCACTAAGTGGGATCAA+CGG 0.604416 5_2:-15751721 Msa0759860:five_prime_UTR
TGAATCCGGCGGGTTTAACA+GGG 0.604481 5_2:-15751650 Msa0759860:CDS
AGCAGAAGGATGAATCCGGC+GGG 0.612519 5_2:-15751660 Msa0759860:exon
AATATTGTCATTGGACCACA+AGG 0.613637 5_2:+15750233 None:intergenic
TTCTTCGGCGGATAACACAA+AGG 0.617908 5_2:-15750457 Msa0759860:CDS
AAATAGGAAGTGGAGGCATG+TGG 0.631407 5_2:-15750405 Msa0759860:exon
AATTTGCAAACACTGTTGCG+TGG 0.632781 5_2:-15750570 Msa0759860:CDS
TTGCGTGGCAGAAACAGCGT+TGG 0.639499 5_2:-15750555 Msa0759860:CDS
ATTTCGGAACGAGATTGATG+AGG 0.642540 5_2:+15751522 None:intergenic
CTGTCTCCGGAATTCAGCCA+GGG 0.642587 5_2:-15750510 Msa0759860:CDS
AGGGCGAAGGAGCATGTCGA+CGG 0.655015 5_2:-15751631 Msa0759860:CDS
CAGCAGAAGGATGAATCCGG+CGG 0.656372 5_2:-15751661 Msa0759860:exon
TACTAATACGGATTCTTCGG+CGG 0.662646 5_2:-15750469 Msa0759860:CDS
CCTTGATATTAATCGCACCC+TGG 0.662781 5_2:+15750493 None:intergenic
GGCAGCACATCACAAATCAG+AGG 0.678094 5_2:-15751700 Msa0759860:five_prime_UTR
CTTCGGCGGATAACACAAAG+GGG 0.722706 5_2:-15750455 Msa0759860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAACAAAAAGAAAAAA+GGG + chr5_2:15750407-15750426 None:intergenic 15.0%
!! TAATGAAACAAAAAGAAAAA+AGG + chr5_2:15750408-15750427 None:intergenic 15.0%
!!! CTTTATTTTATCTTTTGAAT+TGG - chr5_2:15750561-15750580 Msa0759860:CDS 15.0%
!!! TTTATTTACTTTTGCTTATT+TGG - chr5_2:15751196-15751215 Msa0759860:intron 15.0%
!! TGAACTAAATAATAACTATT+AGG - chr5_2:15751756-15751775 Msa0759860:five_prime_UTR 15.0%
!! AATGAAACAAAAAGAAAAAA+GGG + chr5_2:15750407-15750426 None:intergenic 15.0%
!! TAATGAAACAAAAAGAAAAA+AGG + chr5_2:15750408-15750427 None:intergenic 15.0%
!!! CTTTATTTTATCTTTTGAAT+TGG - chr5_2:15750561-15750580 Msa0759860:CDS 15.0%
!!! TTTATTTACTTTTGCTTATT+TGG - chr5_2:15751196-15751215 Msa0759860:intron 15.0%
!! TCAAATAAACCAATTCAAAT+AGG + chr5_2:15750614-15750633 None:intergenic 20.0%
!! AACTTATAAAAGCAACTTAT+AGG - chr5_2:15750747-15750766 Msa0759860:intron 20.0%
!! ATTAAGCTGTTTATAAAAAC+AGG - chr5_2:15750987-15751006 Msa0759860:intron 20.0%
!! TTAAGCTGTTTATAAAAACA+GGG - chr5_2:15750988-15751007 Msa0759860:intron 20.0%
!! CTTTCCATAAAATAAGATAT+AGG + chr5_2:15751014-15751033 None:intergenic 20.0%
!! TAATATCAAGGATACTAATA+CGG - chr5_2:15751367-15751386 Msa0759860:intron 20.0%
!!! TTTTTTTTCTATTCCTAGAT+AGG - chr5_2:15751470-15751489 Msa0759860:intron 20.0%
!! AAAGTTTCAAAAATCTAACT+TGG + chr5_2:15751498-15751517 None:intergenic 20.0%
!! TCAAATAAACCAATTCAAAT+AGG + chr5_2:15750614-15750633 None:intergenic 20.0%
!! AACTTATAAAAGCAACTTAT+AGG - chr5_2:15750747-15750766 Msa0759860:intron 20.0%
!! ATTAAGCTGTTTATAAAAAC+AGG - chr5_2:15750987-15751006 Msa0759860:intron 20.0%
!! TTAAGCTGTTTATAAAAACA+GGG - chr5_2:15750988-15751007 Msa0759860:intron 20.0%
!! CTTTCCATAAAATAAGATAT+AGG + chr5_2:15751014-15751033 None:intergenic 20.0%
!! TAATATCAAGGATACTAATA+CGG - chr5_2:15751367-15751386 Msa0759860:intron 20.0%
!!! TTTTTTTTCTATTCCTAGAT+AGG - chr5_2:15751470-15751489 Msa0759860:intron 20.0%
!! AAAGTTTCAAAAATCTAACT+TGG + chr5_2:15751498-15751517 None:intergenic 20.0%
!! TTTTGTTAGTCCAAAACAAA+CGG - chr5_2:15750107-15750126 Msa0759860:three_prime_UTR 25.0%
! TGAAAATAAGCTGAAAAACA+TGG + chr5_2:15750484-15750503 None:intergenic 25.0%
!!! TATTTGAGCTTATTTTATCC+AGG - chr5_2:15750586-15750605 Msa0759860:CDS 25.0%
! AATAAGGTAAACACAACTTA+TGG + chr5_2:15750709-15750728 None:intergenic 25.0%
! TATAAAAGCAACTTATAGGT+TGG - chr5_2:15750751-15750770 Msa0759860:intron 25.0%
!! TACTTTTGCTTATTTGGAAA+TGG - chr5_2:15751202-15751221 Msa0759860:intron 25.0%
! TATTAAGAAAGACAAGCTTT+GGG - chr5_2:15751250-15751269 Msa0759860:intron 25.0%
!!! TTTGAGTATTGAAACTAGTT+TGG - chr5_2:15751586-15751605 Msa0759860:CDS 25.0%
! CAGAAAATGAATATTGTCAT+TGG + chr5_2:15751627-15751646 None:intergenic 25.0%
!! TTTTGTTAGTCCAAAACAAA+CGG - chr5_2:15750107-15750126 Msa0759860:three_prime_UTR 25.0%
! TGAAAATAAGCTGAAAAACA+TGG + chr5_2:15750484-15750503 None:intergenic 25.0%
!!! TATTTGAGCTTATTTTATCC+AGG - chr5_2:15750586-15750605 Msa0759860:CDS 25.0%
! AATAAGGTAAACACAACTTA+TGG + chr5_2:15750709-15750728 None:intergenic 25.0%
! TATAAAAGCAACTTATAGGT+TGG - chr5_2:15750751-15750770 Msa0759860:intron 25.0%
!! TACTTTTGCTTATTTGGAAA+TGG - chr5_2:15751202-15751221 Msa0759860:intron 25.0%
! TATTAAGAAAGACAAGCTTT+GGG - chr5_2:15751250-15751269 Msa0759860:intron 25.0%
!!! TTTGAGTATTGAAACTAGTT+TGG - chr5_2:15751586-15751605 Msa0759860:CDS 25.0%
! CAGAAAATGAATATTGTCAT+TGG + chr5_2:15751627-15751646 None:intergenic 25.0%
TTTGTTAGTCCAAAACAAAC+GGG - chr5_2:15750108-15750127 Msa0759860:three_prime_UTR 30.0%
TTTGATGAACTTGGAAATGA+TGG + chr5_2:15750357-15750376 None:intergenic 30.0%
CTTAGAGAGCTTATGAAATA+AGG + chr5_2:15750725-15750744 None:intergenic 30.0%
TTGAGAGAACTTATGAAAAC+AGG - chr5_2:15750827-15750846 Msa0759860:intron 30.0%
! AGCTATAAGCTCTTTTCATA+CGG + chr5_2:15750908-15750927 None:intergenic 30.0%
!!! AGGGCCTATATCTTATTTTA+TGG - chr5_2:15751007-15751026 Msa0759860:intron 30.0%
GGATAAACAACTTCATTAAG+TGG + chr5_2:15751120-15751139 None:intergenic 30.0%
GCTTATTTGGAAATGGTATA+TGG - chr5_2:15751209-15751228 Msa0759860:intron 30.0%
GTATTAAGAAAGACAAGCTT+TGG - chr5_2:15751249-15751268 Msa0759860:intron 30.0%
GAAGAAAGGGAAAAAGAAAT+AGG - chr5_2:15751427-15751446 Msa0759860:intron 30.0%
CTGCATGATAATTTCATGAA+TGG - chr5_2:15751543-15751562 Msa0759860:CDS 30.0%
! ATAGCCAAACTAGTTTCAAA+CGG + chr5_2:15751662-15751681 None:intergenic 30.0%
TTTGTTAGTCCAAAACAAAC+GGG - chr5_2:15750108-15750127 Msa0759860:three_prime_UTR 30.0%
TTTGATGAACTTGGAAATGA+TGG + chr5_2:15750357-15750376 None:intergenic 30.0%
CTTAGAGAGCTTATGAAATA+AGG + chr5_2:15750725-15750744 None:intergenic 30.0%
TTGAGAGAACTTATGAAAAC+AGG - chr5_2:15750827-15750846 Msa0759860:intron 30.0%
! AGCTATAAGCTCTTTTCATA+CGG + chr5_2:15750908-15750927 None:intergenic 30.0%
!!! AGGGCCTATATCTTATTTTA+TGG - chr5_2:15751007-15751026 Msa0759860:intron 30.0%
GGATAAACAACTTCATTAAG+TGG + chr5_2:15751120-15751139 None:intergenic 30.0%
GCTTATTTGGAAATGGTATA+TGG - chr5_2:15751209-15751228 Msa0759860:intron 30.0%
GTATTAAGAAAGACAAGCTT+TGG - chr5_2:15751249-15751268 Msa0759860:intron 30.0%
GAAGAAAGGGAAAAAGAAAT+AGG - chr5_2:15751427-15751446 Msa0759860:intron 30.0%
CTGCATGATAATTTCATGAA+TGG - chr5_2:15751543-15751562 Msa0759860:CDS 30.0%
! ATAGCCAAACTAGTTTCAAA+CGG + chr5_2:15751662-15751681 None:intergenic 30.0%
! GGAAATGATGGAAGCAATTT+CGG + chr5_2:15750345-15750364 None:intergenic 35.0%
ACTGTAGAGTTTGATGAACT+TGG + chr5_2:15750366-15750385 None:intergenic 35.0%
TCATCAAACTCTACAGTTTC+CGG - chr5_2:15750369-15750388 Msa0759860:three_prime_UTR 35.0%
AAAAGGGTAGAAATGAAACC+CGG + chr5_2:15750391-15750410 None:intergenic 35.0%
GAGAACATTGATCAAACAGT+TGG + chr5_2:15750438-15750457 None:intergenic 35.0%
! AATGATGGCATGTTTTGTGA+GGG + chr5_2:15750460-15750479 None:intergenic 35.0%
! AAATGATGGCATGTTTTGTG+AGG + chr5_2:15750461-15750480 None:intergenic 35.0%
GCTGAAAAACATGGAAATGA+TGG + chr5_2:15750475-15750494 None:intergenic 35.0%
CAAGTGCTTGTTTCAATACA+TGG + chr5_2:15750643-15750662 None:intergenic 35.0%
!! AAGCAACTTATAGGTTGGTT+TGG - chr5_2:15750756-15750775 Msa0759860:intron 35.0%
AATGACAACAGCTCTTACTT+AGG + chr5_2:15751150-15751169 None:intergenic 35.0%
AGCTTGTCTTTCTTAATACC+AGG + chr5_2:15751248-15751267 None:intergenic 35.0%
AGACAGAATCTTAGCAATCT+CGG + chr5_2:15751324-15751343 None:intergenic 35.0%
GGATACTAATACGGATTCTT+CGG - chr5_2:15751376-15751395 Msa0759860:intron 35.0%
! TCTTTTTCCCTTTCTTCTGT+TGG + chr5_2:15751424-15751443 None:intergenic 35.0%
AGGGAAAAAGAAATAGGAAG+TGG - chr5_2:15751433-15751452 Msa0759860:intron 35.0%
GAAAAAGAAATAGGAAGTGG+AGG - chr5_2:15751436-15751455 Msa0759860:intron 35.0%
ATCTAACTTGGTTCCTATCT+AGG + chr5_2:15751486-15751505 None:intergenic 35.0%
AATATTGTCATTGGACCACA+AGG + chr5_2:15751618-15751637 None:intergenic 35.0%
! TAGCCAAACTAGTTTCAAAC+GGG + chr5_2:15751661-15751680 None:intergenic 35.0%
! GGAAATGATGGAAGCAATTT+CGG + chr5_2:15750345-15750364 None:intergenic 35.0%
ACTGTAGAGTTTGATGAACT+TGG + chr5_2:15750366-15750385 None:intergenic 35.0%
TCATCAAACTCTACAGTTTC+CGG - chr5_2:15750369-15750388 Msa0759860:three_prime_UTR 35.0%
AAAAGGGTAGAAATGAAACC+CGG + chr5_2:15750391-15750410 None:intergenic 35.0%
GAGAACATTGATCAAACAGT+TGG + chr5_2:15750438-15750457 None:intergenic 35.0%
! AATGATGGCATGTTTTGTGA+GGG + chr5_2:15750460-15750479 None:intergenic 35.0%
! AAATGATGGCATGTTTTGTG+AGG + chr5_2:15750461-15750480 None:intergenic 35.0%
GCTGAAAAACATGGAAATGA+TGG + chr5_2:15750475-15750494 None:intergenic 35.0%
CAAGTGCTTGTTTCAATACA+TGG + chr5_2:15750643-15750662 None:intergenic 35.0%
!! AAGCAACTTATAGGTTGGTT+TGG - chr5_2:15750756-15750775 Msa0759860:intron 35.0%
AATGACAACAGCTCTTACTT+AGG + chr5_2:15751150-15751169 None:intergenic 35.0%
AGCTTGTCTTTCTTAATACC+AGG + chr5_2:15751248-15751267 None:intergenic 35.0%
AGACAGAATCTTAGCAATCT+CGG + chr5_2:15751324-15751343 None:intergenic 35.0%
GGATACTAATACGGATTCTT+CGG - chr5_2:15751376-15751395 Msa0759860:intron 35.0%
! TCTTTTTCCCTTTCTTCTGT+TGG + chr5_2:15751424-15751443 None:intergenic 35.0%
AGGGAAAAAGAAATAGGAAG+TGG - chr5_2:15751433-15751452 Msa0759860:intron 35.0%
GAAAAAGAAATAGGAAGTGG+AGG - chr5_2:15751436-15751455 Msa0759860:intron 35.0%
ATCTAACTTGGTTCCTATCT+AGG + chr5_2:15751486-15751505 None:intergenic 35.0%
AATATTGTCATTGGACCACA+AGG + chr5_2:15751618-15751637 None:intergenic 35.0%
! TAGCCAAACTAGTTTCAAAC+GGG + chr5_2:15751661-15751680 None:intergenic 35.0%
AAAACAAACGGGCACTAAGT+GGG - chr5_2:15750119-15750138 Msa0759860:three_prime_UTR 40.0%
GAAGACAAAAATCCAGCAGA+AGG - chr5_2:15750174-15750193 Msa0759860:three_prime_UTR 40.0%
TTTGCCGACACCCATAATTT+CGG + chr5_2:15750258-15750277 None:intergenic 40.0%
AGTGAACTCTGCACTTTCAT+GGG - chr5_2:15750299-15750318 Msa0759860:three_prime_UTR 40.0%
ATTTCGGAACGAGATTGATG+AGG + chr5_2:15750329-15750348 None:intergenic 40.0%
CATCAAACTCTACAGTTTCC+GGG - chr5_2:15750370-15750389 Msa0759860:three_prime_UTR 40.0%
ATCCAGGAGCCTATTTGAAT+TGG - chr5_2:15750602-15750621 Msa0759860:CDS 40.0%
AACCAATTCAAATAGGCTCC+TGG + chr5_2:15750607-15750626 None:intergenic 40.0%
AGCTCTTACTTAGGCTCATT+TGG + chr5_2:15751141-15751160 None:intergenic 40.0%
AATTTGCAAACACTGTTGCG+TGG - chr5_2:15751278-15751297 Msa0759860:intron 40.0%
GACAGAATCTTAGCAATCTC+GGG + chr5_2:15751323-15751342 None:intergenic 40.0%
GATTGCTAAGATTCTGTCTC+CGG - chr5_2:15751325-15751344 Msa0759860:intron 40.0%
TACTAATACGGATTCTTCGG+CGG - chr5_2:15751379-15751398 Msa0759860:intron 40.0%
! TGTCCCGTTTGAAACTAGTT+TGG - chr5_2:15751655-15751674 Msa0759860:exon 40.0%
AAAACAAACGGGCACTAAGT+GGG - chr5_2:15750119-15750138 Msa0759860:three_prime_UTR 40.0%
GAAGACAAAAATCCAGCAGA+AGG - chr5_2:15750174-15750193 Msa0759860:three_prime_UTR 40.0%
TTTGCCGACACCCATAATTT+CGG + chr5_2:15750258-15750277 None:intergenic 40.0%
AGTGAACTCTGCACTTTCAT+GGG - chr5_2:15750299-15750318 Msa0759860:three_prime_UTR 40.0%
ATTTCGGAACGAGATTGATG+AGG + chr5_2:15750329-15750348 None:intergenic 40.0%
CATCAAACTCTACAGTTTCC+GGG - chr5_2:15750370-15750389 Msa0759860:three_prime_UTR 40.0%
ATCCAGGAGCCTATTTGAAT+TGG - chr5_2:15750602-15750621 Msa0759860:CDS 40.0%
AACCAATTCAAATAGGCTCC+TGG + chr5_2:15750607-15750626 None:intergenic 40.0%
AGCTCTTACTTAGGCTCATT+TGG + chr5_2:15751141-15751160 None:intergenic 40.0%
AATTTGCAAACACTGTTGCG+TGG - chr5_2:15751278-15751297 Msa0759860:intron 40.0%
GACAGAATCTTAGCAATCTC+GGG + chr5_2:15751323-15751342 None:intergenic 40.0%
GATTGCTAAGATTCTGTCTC+CGG - chr5_2:15751325-15751344 Msa0759860:intron 40.0%
TACTAATACGGATTCTTCGG+CGG - chr5_2:15751379-15751398 Msa0759860:intron 40.0%
! TGTCCCGTTTGAAACTAGTT+TGG - chr5_2:15751655-15751674 Msa0759860:exon 40.0%
! CACTTAGTGCCCGTTTGTTT+TGG + chr5_2:15750120-15750139 None:intergenic 45.0%
CAAAACAAACGGGCACTAAG+TGG - chr5_2:15750118-15750137 Msa0759860:three_prime_UTR 45.0%
ATCCAGCAGAAGGATGAATC+CGG - chr5_2:15750184-15750203 Msa0759860:three_prime_UTR 45.0%
AAAGCCGTCGCCGAAATTAT+GGG - chr5_2:15750245-15750264 Msa0759860:three_prime_UTR 45.0%
! CATGAAAGTGCAGAGTTCAC+TGG + chr5_2:15750300-15750319 None:intergenic 45.0%
CAGTGAACTCTGCACTTTCA+TGG - chr5_2:15750298-15750317 Msa0759860:three_prime_UTR 45.0%
CCTTGATATTAATCGCACCC+TGG + chr5_2:15751358-15751377 None:intergenic 45.0%
CCAGGGTGCGATTAATATCA+AGG - chr5_2:15751355-15751374 Msa0759860:intron 45.0%
TTCTTCGGCGGATAACACAA+AGG - chr5_2:15751391-15751410 Msa0759860:intron 45.0%
TCTTCGGCGGATAACACAAA+GGG - chr5_2:15751392-15751411 Msa0759860:intron 45.0%
GGAAAGGCCAACAGAAGAAA+GGG - chr5_2:15751414-15751433 Msa0759860:intron 45.0%
! AAATAGGAAGTGGAGGCATG+TGG - chr5_2:15751443-15751462 Msa0759860:intron 45.0%
CTAGTTTGGCTATTGCCTTG+TGG - chr5_2:15751600-15751619 Msa0759860:CDS 45.0%
! CACTTAGTGCCCGTTTGTTT+TGG + chr5_2:15750120-15750139 None:intergenic 45.0%
CAAAACAAACGGGCACTAAG+TGG - chr5_2:15750118-15750137 Msa0759860:three_prime_UTR 45.0%
ATCCAGCAGAAGGATGAATC+CGG - chr5_2:15750184-15750203 Msa0759860:three_prime_UTR 45.0%
AAAGCCGTCGCCGAAATTAT+GGG - chr5_2:15750245-15750264 Msa0759860:three_prime_UTR 45.0%
! CATGAAAGTGCAGAGTTCAC+TGG + chr5_2:15750300-15750319 None:intergenic 45.0%
CAGTGAACTCTGCACTTTCA+TGG - chr5_2:15750298-15750317 Msa0759860:three_prime_UTR 45.0%
CCTTGATATTAATCGCACCC+TGG + chr5_2:15751358-15751377 None:intergenic 45.0%
CCAGGGTGCGATTAATATCA+AGG - chr5_2:15751355-15751374 Msa0759860:intron 45.0%
TTCTTCGGCGGATAACACAA+AGG - chr5_2:15751391-15751410 Msa0759860:intron 45.0%
TCTTCGGCGGATAACACAAA+GGG - chr5_2:15751392-15751411 Msa0759860:intron 45.0%
GGAAAGGCCAACAGAAGAAA+GGG - chr5_2:15751414-15751433 Msa0759860:intron 45.0%
! AAATAGGAAGTGGAGGCATG+TGG - chr5_2:15751443-15751462 Msa0759860:intron 45.0%
CTAGTTTGGCTATTGCCTTG+TGG - chr5_2:15751600-15751619 Msa0759860:CDS 45.0%
GGCAGCACATCACAAATCAG+AGG - chr5_2:15750148-15750167 Msa0759860:three_prime_UTR 50.0%
! ATGAATCCGGCGGGTTTAAC+AGG - chr5_2:15750197-15750216 Msa0759860:three_prime_UTR 50.0%
! TGAATCCGGCGGGTTTAACA+GGG - chr5_2:15750198-15750217 Msa0759860:three_prime_UTR 50.0%
CATAATTTCGGCGACGGCTT+TGG + chr5_2:15750246-15750265 None:intergenic 50.0%
CAAAGCCGTCGCCGAAATTA+TGG - chr5_2:15750244-15750263 Msa0759860:three_prime_UTR 50.0%
GACACCCATAATTTCGGCGA+CGG + chr5_2:15750252-15750271 None:intergenic 50.0%
GTCGCCGAAATTATGGGTGT+CGG - chr5_2:15750251-15750270 Msa0759860:three_prime_UTR 50.0%
!! TATGGTGTTGTGCAGAGTCC+TGG - chr5_2:15751227-15751246 Msa0759860:intron 50.0%
CTTCGGCGGATAACACAAAG+GGG - chr5_2:15751393-15751412 Msa0759860:intron 50.0%
GCGGATAACACAAAGGGGAA+AGG - chr5_2:15751398-15751417 Msa0759860:intron 50.0%
GGGAAAGGCCAACAGAAGAA+AGG - chr5_2:15751413-15751432 Msa0759860:intron 50.0%
GGCAGCACATCACAAATCAG+AGG - chr5_2:15750148-15750167 Msa0759860:three_prime_UTR 50.0%
! ATGAATCCGGCGGGTTTAAC+AGG - chr5_2:15750197-15750216 Msa0759860:three_prime_UTR 50.0%
! TGAATCCGGCGGGTTTAACA+GGG - chr5_2:15750198-15750217 Msa0759860:three_prime_UTR 50.0%
CATAATTTCGGCGACGGCTT+TGG + chr5_2:15750246-15750265 None:intergenic 50.0%
CAAAGCCGTCGCCGAAATTA+TGG - chr5_2:15750244-15750263 Msa0759860:three_prime_UTR 50.0%
GACACCCATAATTTCGGCGA+CGG + chr5_2:15750252-15750271 None:intergenic 50.0%
GTCGCCGAAATTATGGGTGT+CGG - chr5_2:15750251-15750270 Msa0759860:three_prime_UTR 50.0%
!! TATGGTGTTGTGCAGAGTCC+TGG - chr5_2:15751227-15751246 Msa0759860:intron 50.0%
CTTCGGCGGATAACACAAAG+GGG - chr5_2:15751393-15751412 Msa0759860:intron 50.0%
GCGGATAACACAAAGGGGAA+AGG - chr5_2:15751398-15751417 Msa0759860:intron 50.0%
GGGAAAGGCCAACAGAAGAA+AGG - chr5_2:15751413-15751432 Msa0759860:intron 50.0%
CGGGCACTAAGTGGGATCAA+CGG - chr5_2:15750127-15750146 Msa0759860:three_prime_UTR 55.0%
CAGCAGAAGGATGAATCCGG+CGG - chr5_2:15750187-15750206 Msa0759860:three_prime_UTR 55.0%
AGCAGAAGGATGAATCCGGC+GGG - chr5_2:15750188-15750207 Msa0759860:three_prime_UTR 55.0%
! ACTGGTAGCTTTGCGCGTCT+TGG + chr5_2:15750282-15750301 None:intergenic 55.0%
TTGCGTGGCAGAAACAGCGT+TGG - chr5_2:15751293-15751312 Msa0759860:intron 55.0%
TCTGTCTCCGGAATTCAGCC+AGG - chr5_2:15751337-15751356 Msa0759860:intron 55.0%
CTGTCTCCGGAATTCAGCCA+GGG - chr5_2:15751338-15751357 Msa0759860:intron 55.0%
ATCGCACCCTGGCTGAATTC+CGG + chr5_2:15751347-15751366 None:intergenic 55.0%
! AGGAAGTGGAGGCATGTGGT+TGG - chr5_2:15751447-15751466 Msa0759860:intron 55.0%
CGGGCACTAAGTGGGATCAA+CGG - chr5_2:15750127-15750146 Msa0759860:three_prime_UTR 55.0%
CAGCAGAAGGATGAATCCGG+CGG - chr5_2:15750187-15750206 Msa0759860:three_prime_UTR 55.0%
AGCAGAAGGATGAATCCGGC+GGG - chr5_2:15750188-15750207 Msa0759860:three_prime_UTR 55.0%
! ACTGGTAGCTTTGCGCGTCT+TGG + chr5_2:15750282-15750301 None:intergenic 55.0%
TTGCGTGGCAGAAACAGCGT+TGG - chr5_2:15751293-15751312 Msa0759860:intron 55.0%
TCTGTCTCCGGAATTCAGCC+AGG - chr5_2:15751337-15751356 Msa0759860:intron 55.0%
CTGTCTCCGGAATTCAGCCA+GGG - chr5_2:15751338-15751357 Msa0759860:intron 55.0%
ATCGCACCCTGGCTGAATTC+CGG + chr5_2:15751347-15751366 None:intergenic 55.0%
! AGGAAGTGGAGGCATGTGGT+TGG - chr5_2:15751447-15751466 Msa0759860:intron 55.0%
CGCCGGATTCATCCTTCTGC+TGG + chr5_2:15750189-15750208 None:intergenic 60.0%
CTTCGCCCTGTTAAACCCGC+CGG + chr5_2:15750206-15750225 None:intergenic 60.0%
! AGGGCGAAGGAGCATGTCGA+CGG - chr5_2:15750217-15750236 Msa0759860:three_prime_UTR 60.0%
CGCCGGATTCATCCTTCTGC+TGG + chr5_2:15750189-15750208 None:intergenic 60.0%
CTTCGCCCTGTTAAACCCGC+CGG + chr5_2:15750206-15750225 None:intergenic 60.0%
! AGGGCGAAGGAGCATGTCGA+CGG - chr5_2:15750217-15750236 Msa0759860:three_prime_UTR 60.0%
! CGGCGGGTTTAACAGGGCGA+AGG - chr5_2:15750204-15750223 Msa0759860:three_prime_UTR 65.0%
! CGGCGGGTTTAACAGGGCGA+AGG - chr5_2:15750204-15750223 Msa0759860:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 15750095 15751775 15750095 ID=Msa0759860;Name=Msa0759860
chr5_2 mRNA 15750095 15751775 15750095 ID=Msa0759860-mRNA-1;Parent=Msa0759860;Name=Msa0759860-mRNA-1;_AED=0.00;_eAED=0.00;_QI=102|1|1|1|1|1|2|327|133
chr5_2 exon 15750095 15750628 15750095 ID=Msa0759860-mRNA-1:exon:7132;Parent=Msa0759860-mRNA-1
chr5_2 exon 15751479 15751775 15751479 ID=Msa0759860-mRNA-1:exon:7131;Parent=Msa0759860-mRNA-1
chr5_2 five_prime_UTR 15751674 15751775 15751674 ID=Msa0759860-mRNA-1:five_prime_utr;Parent=Msa0759860-mRNA-1
chr5_2 CDS 15751479 15751673 15751479 ID=Msa0759860-mRNA-1:cds;Parent=Msa0759860-mRNA-1
chr5_2 CDS 15750422 15750628 15750422 ID=Msa0759860-mRNA-1:cds;Parent=Msa0759860-mRNA-1
chr5_2 three_prime_UTR 15750095 15750421 15750095 ID=Msa0759860-mRNA-1:three_prime_utr;Parent=Msa0759860-mRNA-1
Gene Sequence

>Msa0759860

ATGAATCCGGCGGGTTTAACAGGGCGAAGGAGCATGTCGACGGTATCCAAAGCCGTCGCCGAAATTATGGGTGTCGGCAAAACCAAGACGCGCAAAGCTACCAGTGAACTCTGCACTTTCATGGGCATTCCTCATCAATCTCGTTCCGAAATTGCTTCCATCATTTCCAAGTTCATCAAACTCTACAGTTTCCGGAGTCCTGGTATTAAGAAAGACAAGCTTTGGGAGCAGAATTTGCAAACACTGTTGCGTGGCAGAAACAGCGTTGGTTTTCCCGAGATTGCTAAGATTCTGTCTCCGGAATTCAGCCAGGGTGCGATTAATATCAAGGATACTAATACGGATTCTTCGGCGGATAACACAAAGGGGAAAGGCCAACAGAAGAAAGGGAAAAAGAAATAG
Protein sequence

>Msa0759860

MNPAGLTGRRSMSTVSKAVAEIMGVGKTKTRKATSELCTFMGIPHQSRSEIASIISKFIKLYSFRSPGIKKDKLWEQNLQTLLRGRNSVGFPEIAKILSPEFSQGAINIKDTNTDSSADNTKGKGQQKKGKKK*