Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0832040 | XP_024630855.2 | 65.079 | 63 | 19 | 1 | 10 | 72 | 121 | 180 | 1.75e-12 | 70.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0832040 | A0A072VP56 | 65.079 | 63 | 19 | 1 | 10 | 72 | 229 | 288 | 1.28e-12 | 71.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014710 | Msa0832040 | 0.826006 | 3.437205e-54 | -8.615850e-47 |
| Msa0031520 | Msa0832040 | 0.803728 | 3.035441e-49 | -8.615850e-47 |
| Msa0047280 | Msa0832040 | 0.806241 | 9.049360e-50 | -8.615850e-47 |
| Msa0832030 | Msa0832040 | 0.830411 | 2.981075e-55 | -8.615850e-47 |
| Msa0832040 | Msa0832050 | 0.824649 | 7.200774e-54 | -8.615850e-47 |
| Msa0832040 | Msa0844410 | 0.810320 | 1.222526e-50 | -8.615850e-47 |
| Msa0832040 | Msa0844420 | 0.832496 | 9.144642e-56 | -8.615850e-47 |
| Msa0832040 | Msa0844440 | 0.806286 | 8.855427e-50 | -8.615850e-47 |
| Msa0832040 | Msa0844470 | 0.806610 | 7.565249e-50 | -8.615850e-47 |
| Msa0832040 | Msa0844490 | 0.811607 | 6.432604e-51 | -8.615850e-47 |
| Msa0832040 | Msa0857640 | 0.822455 | 2.347111e-53 | -8.615850e-47 |
| Msa0832040 | Msa0969520 | 0.802574 | 5.259485e-49 | -8.615850e-47 |
| Msa0832040 | Msa1035660 | 0.819735 | 9.936645e-53 | -8.615850e-47 |
| Msa0832040 | Msa1075540 | 0.802075 | 6.663368e-49 | -8.615850e-47 |
| Msa0832040 | Msa1087480 | 0.819754 | 9.837516e-53 | -8.615850e-47 |
| Msa0832040 | Msa1306760 | 0.819433 | 1.164676e-52 | -8.615850e-47 |
| Msa0832040 | Msa1313320 | 0.807529 | 4.834149e-50 | -8.615850e-47 |
| Msa0832040 | Msa1366970 | 0.813847 | 2.080270e-51 | -8.615850e-47 |
| Msa0832040 | Msa1388160 | 0.802463 | 5.542865e-49 | -8.615850e-47 |
| Msa0832040 | Msa1390700 | 0.808539 | 2.946956e-50 | -8.615850e-47 |
| Msa0832040 | Msa1392910 | 0.804526 | 2.070627e-49 | -8.615850e-47 |
| Msa0832040 | Msa1412530 | 0.819419 | 1.173133e-52 | -8.615850e-47 |
| Msa0139990 | Msa0832040 | 0.809515 | 1.821929e-50 | -8.615850e-47 |
| Msa0150270 | Msa0832040 | 0.807500 | 4.903270e-50 | -8.615850e-47 |
| Msa0171140 | Msa0832040 | 0.829843 | 4.101907e-55 | -8.615850e-47 |
| Msa0180500 | Msa0832040 | 0.816106 | 6.562350e-52 | -8.615850e-47 |
| Msa0259210 | Msa0832040 | 0.821291 | 4.365537e-53 | -8.615850e-47 |
| Msa0259370 | Msa0832040 | 0.827263 | 1.723210e-54 | -8.615850e-47 |
| Msa0278110 | Msa0832040 | 0.813476 | 2.510663e-51 | -8.615850e-47 |
| Msa0287350 | Msa0832040 | 0.810314 | 1.225963e-50 | -8.615850e-47 |
| Msa0298460 | Msa0832040 | 0.807464 | 4.991270e-50 | -8.615850e-47 |
| Msa0336310 | Msa0832040 | 0.803090 | 4.114348e-49 | -8.615850e-47 |
| Msa0336320 | Msa0832040 | 0.817085 | 3.959659e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATTTCTGCAGTTTATAA+AGG | 0.303277 | 5_4:-5139617 | Msa0832040:intron |
| CGAAGTTGCTGCTGCTGATT+CGG | 0.386996 | 5_4:-5139504 | Msa0832040:CDS |
| GACTCCAACTAGCAAACCTT+TGG | 0.409498 | 5_4:-5139477 | Msa0832040:CDS |
| TTTCCCAAAGGTTTGCTAGT+TGG | 0.503027 | 5_4:+5139473 | None:intergenic |
| ACTCCAACTAGCAAACCTTT+GGG | 0.520423 | 5_4:-5139476 | Msa0832040:CDS |
| CTAGCAAACCTTTGGGAAAG+CGG | 0.578566 | 5_4:-5139469 | Msa0832040:CDS |
| TCAGTAGACCGCTTTCCCAA+AGG | 0.587701 | 5_4:+5139461 | None:intergenic |
| AAAGCGGTCTACTGATAGTG+TGG | 0.622760 | 5_4:-5139453 | Msa0832040:CDS |
| CATGCAGGAAAATATTGAAG+TGG | 0.661188 | 5_4:-5139546 | Msa0832040:intron |
| GTTAGCTTATCAAAATCCTG+TGG | 0.715142 | 5_4:-5139652 | Msa0832040:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGACTTTTACTATAAGTT+GGG | + | chr5_4:5139646-5139665 | None:intergenic | 20.0% |
| !!! | TAAAGACTTTTACTATAAGT+TGG | + | chr5_4:5139647-5139666 | None:intergenic | 20.0% |
| !!! | AAAGACTTTTACTATAAGTT+GGG | + | chr5_4:5139646-5139665 | None:intergenic | 20.0% |
| !!! | TAAAGACTTTTACTATAAGT+TGG | + | chr5_4:5139647-5139666 | None:intergenic | 20.0% |
| ! | GCTCAAAAAAAGAAATCAAA+AGG | + | chr5_4:5138826-5138845 | None:intergenic | 25.0% |
| !!! | TTGATTTCTTTTTTTGAGCT+TGG | - | chr5_4:5138827-5138846 | Msa0832040:intron | 25.0% |
| ! | TGATCAAAAAAATATACCAC+AGG | + | chr5_4:5138911-5138930 | None:intergenic | 25.0% |
| ! | ATCATTTCTGCAGTTTATAA+AGG | - | chr5_4:5138927-5138946 | Msa0832040:intron | 25.0% |
| ! | TTTAAATGCATTATCCACAA+TGG | - | chr5_4:5139663-5139682 | Msa0832040:CDS | 25.0% |
| ! | GCTCAAAAAAAGAAATCAAA+AGG | + | chr5_4:5138826-5138845 | None:intergenic | 25.0% |
| !!! | TTGATTTCTTTTTTTGAGCT+TGG | - | chr5_4:5138827-5138846 | Msa0832040:intron | 25.0% |
| ! | TGATCAAAAAAATATACCAC+AGG | + | chr5_4:5138911-5138930 | None:intergenic | 25.0% |
| ! | ATCATTTCTGCAGTTTATAA+AGG | - | chr5_4:5138927-5138946 | Msa0832040:intron | 25.0% |
| ! | TTTAAATGCATTATCCACAA+TGG | - | chr5_4:5139663-5139682 | Msa0832040:CDS | 25.0% |
| GTAAGTGTTTATGCTATAAG+CGG | + | chr5_4:5139349-5139368 | None:intergenic | 30.0% | |
| ! | TAAGTTTTCTTTAGTTACCC+AGG | - | chr5_4:5139429-5139448 | Msa0832040:intron | 30.0% |
| CTGTATTGAATCAACAAAAC+AGG | - | chr5_4:5139616-5139635 | Msa0832040:intron | 30.0% | |
| ATAGTTAGATAGATCCATTG+TGG | + | chr5_4:5139680-5139699 | None:intergenic | 30.0% | |
| GTAAGTGTTTATGCTATAAG+CGG | + | chr5_4:5139349-5139368 | None:intergenic | 30.0% | |
| ! | TAAGTTTTCTTTAGTTACCC+AGG | - | chr5_4:5139429-5139448 | Msa0832040:intron | 30.0% |
| CTGTATTGAATCAACAAAAC+AGG | - | chr5_4:5139616-5139635 | Msa0832040:intron | 30.0% | |
| ATAGTTAGATAGATCCATTG+TGG | + | chr5_4:5139680-5139699 | None:intergenic | 30.0% | |
| ! | GTTAGCTTATCAAAATCCTG+TGG | - | chr5_4:5138892-5138911 | Msa0832040:intron | 35.0% |
| !! | GCATAATTTTCAGCTTGCTT+CGG | + | chr5_4:5138963-5138982 | None:intergenic | 35.0% |
| CATGCAGGAAAATATTGAAG+TGG | - | chr5_4:5138998-5139017 | Msa0832040:intron | 35.0% | |
| TTGAGTGAATCTATCTCTCT+TGG | + | chr5_4:5139254-5139273 | None:intergenic | 35.0% | |
| TTATTCCCATAAGCTTTCCT+GGG | + | chr5_4:5139449-5139468 | None:intergenic | 35.0% | |
| ACACACTTACGAGATTGTTT+AGG | + | chr5_4:5139519-5139538 | None:intergenic | 35.0% | |
| ! | CTGTAGATACAAAGCTTGTT+TGG | + | chr5_4:5139572-5139591 | None:intergenic | 35.0% |
| ! | GTTAGCTTATCAAAATCCTG+TGG | - | chr5_4:5138892-5138911 | Msa0832040:intron | 35.0% |
| !! | GCATAATTTTCAGCTTGCTT+CGG | + | chr5_4:5138963-5138982 | None:intergenic | 35.0% |
| CATGCAGGAAAATATTGAAG+TGG | - | chr5_4:5138998-5139017 | Msa0832040:intron | 35.0% | |
| TTGAGTGAATCTATCTCTCT+TGG | + | chr5_4:5139254-5139273 | None:intergenic | 35.0% | |
| TTATTCCCATAAGCTTTCCT+GGG | + | chr5_4:5139449-5139468 | None:intergenic | 35.0% | |
| ACACACTTACGAGATTGTTT+AGG | + | chr5_4:5139519-5139538 | None:intergenic | 35.0% | |
| ! | CTGTAGATACAAAGCTTGTT+TGG | + | chr5_4:5139572-5139591 | None:intergenic | 35.0% |
| !! | ATCACTTTTCTCATGCATGC+AGG | - | chr5_4:5138983-5139002 | Msa0832040:intron | 40.0% |
| ACTCCAACTAGCAAACCTTT+GGG | - | chr5_4:5139068-5139087 | Msa0832040:intron | 40.0% | |
| !! | TTTCCCAAAGGTTTGCTAGT+TGG | + | chr5_4:5139074-5139093 | None:intergenic | 40.0% |
| AGTGAATCTATCTCTCTTGG+TGG | + | chr5_4:5139251-5139270 | None:intergenic | 40.0% | |
| TAGTTACCCAGGAAAGCTTA+TGG | - | chr5_4:5139440-5139459 | Msa0832040:CDS | 40.0% | |
| AGTTACCCAGGAAAGCTTAT+GGG | - | chr5_4:5139441-5139460 | Msa0832040:CDS | 40.0% | |
| CTTATTCCCATAAGCTTTCC+TGG | + | chr5_4:5139450-5139469 | None:intergenic | 40.0% | |
| !! | ATCACTTTTCTCATGCATGC+AGG | - | chr5_4:5138983-5139002 | Msa0832040:intron | 40.0% |
| ACTCCAACTAGCAAACCTTT+GGG | - | chr5_4:5139068-5139087 | Msa0832040:intron | 40.0% | |
| !! | TTTCCCAAAGGTTTGCTAGT+TGG | + | chr5_4:5139074-5139093 | None:intergenic | 40.0% |
| AGTGAATCTATCTCTCTTGG+TGG | + | chr5_4:5139251-5139270 | None:intergenic | 40.0% | |
| TAGTTACCCAGGAAAGCTTA+TGG | - | chr5_4:5139440-5139459 | Msa0832040:CDS | 40.0% | |
| AGTTACCCAGGAAAGCTTAT+GGG | - | chr5_4:5139441-5139460 | Msa0832040:CDS | 40.0% | |
| CTTATTCCCATAAGCTTTCC+TGG | + | chr5_4:5139450-5139469 | None:intergenic | 40.0% | |
| !!! | TTTTGAGCTTGGTAACGACG+AGG | - | chr5_4:5138838-5138857 | Msa0832040:intron | 45.0% |
| GACTCCAACTAGCAAACCTT+TGG | - | chr5_4:5139067-5139086 | Msa0832040:intron | 45.0% | |
| ! | CTAGCAAACCTTTGGGAAAG+CGG | - | chr5_4:5139075-5139094 | Msa0832040:intron | 45.0% |
| ! | AAAGCGGTCTACTGATAGTG+TGG | - | chr5_4:5139091-5139110 | Msa0832040:intron | 45.0% |
| ! | ATCTATCTCTCTTGGTGGAC+TGG | + | chr5_4:5139246-5139265 | None:intergenic | 45.0% |
| !!! | TTTTGAGCTTGGTAACGACG+AGG | - | chr5_4:5138838-5138857 | Msa0832040:intron | 45.0% |
| GACTCCAACTAGCAAACCTT+TGG | - | chr5_4:5139067-5139086 | Msa0832040:intron | 45.0% | |
| ! | CTAGCAAACCTTTGGGAAAG+CGG | - | chr5_4:5139075-5139094 | Msa0832040:intron | 45.0% |
| ! | AAAGCGGTCTACTGATAGTG+TGG | - | chr5_4:5139091-5139110 | Msa0832040:intron | 45.0% |
| ! | ATCTATCTCTCTTGGTGGAC+TGG | + | chr5_4:5139246-5139265 | None:intergenic | 45.0% |
| ! | CGAAGTTGCTGCTGCTGATT+CGG | - | chr5_4:5139040-5139059 | Msa0832040:intron | 50.0% |
| TCAGTAGACCGCTTTCCCAA+AGG | + | chr5_4:5139086-5139105 | None:intergenic | 50.0% | |
| ! | CGAAGTTGCTGCTGCTGATT+CGG | - | chr5_4:5139040-5139059 | Msa0832040:intron | 50.0% |
| TCAGTAGACCGCTTTCCCAA+AGG | + | chr5_4:5139086-5139105 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 5138818 | 5139748 | 5138818 | ID=Msa0832040;Name=Msa0832040 |
| chr5_4 | mRNA | 5138818 | 5139748 | 5138818 | ID=Msa0832040-mRNA-1;Parent=Msa0832040;Name=Msa0832040-mRNA-1;_AED=0.47;_eAED=0.72;_QI=0|0|0|0.75|0|0.25|4|0|77 |
| chr5_4 | exon | 5139653 | 5139748 | 5139653 | ID=Msa0832040-mRNA-1:exon:2186;Parent=Msa0832040-mRNA-1 |
| chr5_4 | exon | 5139618 | 5139626 | 5139618 | ID=Msa0832040-mRNA-1:exon:2185;Parent=Msa0832040-mRNA-1 |
| chr5_4 | exon | 5139439 | 5139561 | 5139439 | ID=Msa0832040-mRNA-1:exon:2184;Parent=Msa0832040-mRNA-1 |
| chr5_4 | exon | 5138818 | 5138823 | 5138818 | ID=Msa0832040-mRNA-1:exon:2183;Parent=Msa0832040-mRNA-1 |
| chr5_4 | CDS | 5139653 | 5139748 | 5139653 | ID=Msa0832040-mRNA-1:cds;Parent=Msa0832040-mRNA-1 |
| chr5_4 | CDS | 5139618 | 5139626 | 5139618 | ID=Msa0832040-mRNA-1:cds;Parent=Msa0832040-mRNA-1 |
| chr5_4 | CDS | 5139439 | 5139561 | 5139439 | ID=Msa0832040-mRNA-1:cds;Parent=Msa0832040-mRNA-1 |
| chr5_4 | CDS | 5138818 | 5138823 | 5138818 | ID=Msa0832040-mRNA-1:cds;Parent=Msa0832040-mRNA-1 |
| Gene Sequence |
| Protein sequence |
