AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0150270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTAGACAAATTGAGATTT+AGG 0.164907 1_4:-11534643 Msa0150270:five_prime_UTR
GGTGCCTTTGGGATGCCTTA+TGG 0.225125 1_4:-11534846 Msa0150270:five_prime_UTR
CATAATTTGTGTTAAATATC+TGG 0.231662 1_4:+11534338 None:intergenic
TGTGTTTAAGGTAAAATTTA+TGG 0.254008 1_4:-11534377 Msa0150270:intron
TTAGGAAGCTTAGGCCATTT+TGG 0.272077 1_4:+11534509 None:intergenic
CCTAGACAAATTGAGATTTA+GGG 0.290999 1_4:-11534642 Msa0150270:five_prime_UTR
GTGTCCTTGGTGGGTGCCTT+TGG 0.298783 1_4:-11534858 Msa0150270:five_prime_UTR
AATTCGATTCAAATCTGATT+TGG 0.320135 1_4:-11534994 Msa0150270:five_prime_UTR
ACAAATTATGATTGGGCTCT+TGG 0.345281 1_4:-11534325 Msa0150270:CDS
TTAGCTAGTGTGTTTATGTT+TGG 0.354407 1_4:-11534692 Msa0150270:five_prime_UTR
ACCCTATCATGTATCCAAAA+TGG 0.355033 1_4:-11534523 Msa0150270:CDS
CTAGGATTAAGTCTCACTTC+TGG 0.355710 1_4:+11534660 None:intergenic
GATTTAGGGTGCTTATGAAT+TGG 0.363776 1_4:-11534628 Msa0150270:five_prime_UTR
TATTTAACACAAATTATGAT+TGG 0.366884 1_4:-11534333 Msa0150270:CDS
AGTTAGTAAGTTCTGCAAAA+TGG 0.384552 1_4:-11534946 Msa0150270:five_prime_UTR
TGTCCTTGGTGGGTGCCTTT+GGG 0.399399 1_4:-11534857 Msa0150270:five_prime_UTR
AATTGCTTATAAAACAGATT+AGG 0.403921 1_4:+11534715 None:intergenic
TAAATATCTGGTGATCTTCA+TGG 0.407463 1_4:+11534350 None:intergenic
GTGAAGTTGGCGAAGATCAT+TGG 0.412110 1_4:-11534273 Msa0150270:CDS
GGATTGGTAGCAGGGTAGTT+GGG 0.427021 1_4:-11534572 Msa0150270:five_prime_UTR
AGTAGGAGAGTTAGCTGGAT+TGG 0.428137 1_4:-11534588 Msa0150270:five_prime_UTR
ATTTAACACAAATTATGATT+GGG 0.438388 1_4:-11534332 Msa0150270:CDS
AAACTCACTTGGTTTAATCA+AGG 0.450765 1_4:+11534783 None:intergenic
GAGTTAGCTGGATTGGTAGC+AGG 0.451512 1_4:-11534581 Msa0150270:five_prime_UTR
CATCATCTTCAGCATGTACT+AGG 0.452749 1_4:-11535027 Msa0150270:five_prime_UTR
TAGGATTAAGTCTCACTTCT+GGG 0.455308 1_4:+11534661 None:intergenic
GAGAAATTCCCGAGTGAAGT+TGG 0.457040 1_4:-11534286 Msa0150270:CDS
TGGATTGGTAGCAGGGTAGT+TGG 0.463259 1_4:-11534573 Msa0150270:five_prime_UTR
TATTGTTATTACCAAGCGTC+AGG 0.463749 1_4:+11534223 None:intergenic
TGATCTTCGCCAACTTCACT+CGG 0.479001 1_4:+11534277 None:intergenic
CATTTATGAGAACAACTTGA+AGG 0.482793 1_4:-11534471 Msa0150270:intron
GTTTCTCTGTGTCTGCTGCT+AGG 0.493524 1_4:-11535053 None:intergenic
GGGATGCCTTATGGAGAGGA+TGG 0.494569 1_4:-11534837 Msa0150270:five_prime_UTR
AATAATCCATCCTCTCCATA+AGG 0.516672 1_4:+11534831 None:intergenic
CAGCTAACTCTCCTACTGTC+TGG 0.517203 1_4:+11534594 None:intergenic
TTAGTATCAAATCAAGATCA+AGG 0.520178 1_4:-11534904 Msa0150270:five_prime_UTR
CCCTAAATCTCAATTTGTCT+AGG 0.530507 1_4:+11534642 None:intergenic
AAGTTCTGCAAAATGGTGAT+TGG 0.541266 1_4:-11534939 Msa0150270:five_prime_UTR
AGATCAAGGTGCTTTAATTG+TGG 0.544551 1_4:-11534890 Msa0150270:five_prime_UTR
GAAGATCTAAGTGTCCTTGG+TGG 0.549084 1_4:-11534868 Msa0150270:five_prime_UTR
CTAGTACATGCTGAAGATGA+TGG 0.550331 1_4:+11535028 None:intergenic
ATAGACTGCATCCTGACGCT+TGG 0.551860 1_4:-11534234 Msa0150270:CDS
AGTTAGCTGGATTGGTAGCA+GGG 0.553426 1_4:-11534580 Msa0150270:five_prime_UTR
TGAGATTCTATAAACTCACT+TGG 0.556872 1_4:+11534772 None:intergenic
CAGTCTATAATCAATATACA+TGG 0.558568 1_4:+11534249 None:intergenic
GTGGAAGATCTAAGTGTCCT+TGG 0.564140 1_4:-11534871 Msa0150270:five_prime_UTR
GATTCAAATCTGATTTGGTG+TGG 0.576402 1_4:-11534989 Msa0150270:five_prime_UTR
AAGATCTAAGTGTCCTTGGT+GGG 0.595755 1_4:-11534867 Msa0150270:five_prime_UTR
CAGACAGTAGGAGAGTTAGC+TGG 0.596658 1_4:-11534593 Msa0150270:five_prime_UTR
CTTTGGGATGCCTTATGGAG+AGG 0.614233 1_4:-11534841 Msa0150270:five_prime_UTR
CATCCCAAAGGCACCCACCA+AGG 0.621079 1_4:+11534854 None:intergenic
GATCTTCGCCAACTTCACTC+GGG 0.622921 1_4:+11534278 None:intergenic
AATGCTGAAAGCCAGACAGT+AGG 0.663301 1_4:-11534605 Msa0150270:five_prime_UTR
CTCTCCATAAGGCATCCCAA+AGG 0.664709 1_4:+11534842 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATTTGTTTGTGTTTA+AGG - chr1_4:11534888-11534907 Msa0150270:five_prime_UTR 15.0%
!! TATTTAACACAAATTATGAT+TGG - chr1_4:11534944-11534963 Msa0150270:five_prime_UTR 15.0%
!! ATTTAACACAAATTATGATT+GGG - chr1_4:11534945-11534964 Msa0150270:five_prime_UTR 15.0%
!! TTTATATTTGTTTGTGTTTA+AGG - chr1_4:11534888-11534907 Msa0150270:five_prime_UTR 15.0%
!! TATTTAACACAAATTATGAT+TGG - chr1_4:11534944-11534963 Msa0150270:five_prime_UTR 15.0%
!! ATTTAACACAAATTATGATT+GGG - chr1_4:11534945-11534964 Msa0150270:five_prime_UTR 15.0%
!! AATTGCTTATAAAACAGATT+AGG + chr1_4:11534565-11534584 None:intergenic 20.0%
!!! TGTGTTTAAGGTAAAATTTA+TGG - chr1_4:11534900-11534919 Msa0150270:five_prime_UTR 20.0%
!!! CATAATTTGTGTTAAATATC+TGG + chr1_4:11534942-11534961 None:intergenic 20.0%
!! AATTGCTTATAAAACAGATT+AGG + chr1_4:11534565-11534584 None:intergenic 20.0%
!!! TGTGTTTAAGGTAAAATTTA+TGG - chr1_4:11534900-11534919 Msa0150270:five_prime_UTR 20.0%
!!! CATAATTTGTGTTAAATATC+TGG + chr1_4:11534942-11534961 None:intergenic 20.0%
! AATTCGATTCAAATCTGATT+TGG - chr1_4:11534283-11534302 Msa0150270:CDS 25.0%
! TTAGTATCAAATCAAGATCA+AGG - chr1_4:11534373-11534392 Msa0150270:intron 25.0%
!!! ATGTAGTGTTTCTTTGTTTT+AGG + chr1_4:11534789-11534808 None:intergenic 25.0%
! CAGTCTATAATCAATATACA+TGG + chr1_4:11535031-11535050 None:intergenic 25.0%
! AATTCGATTCAAATCTGATT+TGG - chr1_4:11534283-11534302 Msa0150270:CDS 25.0%
! TTAGTATCAAATCAAGATCA+AGG - chr1_4:11534373-11534392 Msa0150270:intron 25.0%
!!! ATGTAGTGTTTCTTTGTTTT+AGG + chr1_4:11534789-11534808 None:intergenic 25.0%
! CAGTCTATAATCAATATACA+TGG + chr1_4:11535031-11535050 None:intergenic 25.0%
AGTTAGTAAGTTCTGCAAAA+TGG - chr1_4:11534331-11534350 Msa0150270:CDS 30.0%
AAACTCACTTGGTTTAATCA+AGG + chr1_4:11534497-11534516 None:intergenic 30.0%
TGAGATTCTATAAACTCACT+TGG + chr1_4:11534508-11534527 None:intergenic 30.0%
TTAGCTAGTGTGTTTATGTT+TGG - chr1_4:11534585-11534604 Msa0150270:five_prime_UTR 30.0%
TCCTAGACAAATTGAGATTT+AGG - chr1_4:11534634-11534653 Msa0150270:five_prime_UTR 30.0%
CCTAGACAAATTGAGATTTA+GGG - chr1_4:11534635-11534654 Msa0150270:five_prime_UTR 30.0%
!!! TTCTTTGTTTTAGGAAGCTT+AGG + chr1_4:11534780-11534799 None:intergenic 30.0%
CATTTATGAGAACAACTTGA+AGG - chr1_4:11534806-11534825 Msa0150270:five_prime_UTR 30.0%
TAAATATCTGGTGATCTTCA+TGG + chr1_4:11534930-11534949 None:intergenic 30.0%
AGTTAGTAAGTTCTGCAAAA+TGG - chr1_4:11534331-11534350 Msa0150270:CDS 30.0%
AAACTCACTTGGTTTAATCA+AGG + chr1_4:11534497-11534516 None:intergenic 30.0%
TGAGATTCTATAAACTCACT+TGG + chr1_4:11534508-11534527 None:intergenic 30.0%
TTAGCTAGTGTGTTTATGTT+TGG - chr1_4:11534585-11534604 Msa0150270:five_prime_UTR 30.0%
TCCTAGACAAATTGAGATTT+AGG - chr1_4:11534634-11534653 Msa0150270:five_prime_UTR 30.0%
CCTAGACAAATTGAGATTTA+GGG - chr1_4:11534635-11534654 Msa0150270:five_prime_UTR 30.0%
!!! TTCTTTGTTTTAGGAAGCTT+AGG + chr1_4:11534780-11534799 None:intergenic 30.0%
CATTTATGAGAACAACTTGA+AGG - chr1_4:11534806-11534825 Msa0150270:five_prime_UTR 30.0%
TAAATATCTGGTGATCTTCA+TGG + chr1_4:11534930-11534949 None:intergenic 30.0%
!! GATTCAAATCTGATTTGGTG+TGG - chr1_4:11534288-11534307 Msa0150270:CDS 35.0%
AAGTTCTGCAAAATGGTGAT+TGG - chr1_4:11534338-11534357 Msa0150270:CDS 35.0%
! AGATCAAGGTGCTTTAATTG+TGG - chr1_4:11534387-11534406 Msa0150270:intron 35.0%
AATAATCCATCCTCTCCATA+AGG + chr1_4:11534449-11534468 None:intergenic 35.0%
TAGGATTAAGTCTCACTTCT+GGG + chr1_4:11534619-11534638 None:intergenic 35.0%
CCCTAAATCTCAATTTGTCT+AGG + chr1_4:11534638-11534657 None:intergenic 35.0%
! GATTTAGGGTGCTTATGAAT+TGG - chr1_4:11534649-11534668 Msa0150270:five_prime_UTR 35.0%
ACCCTATCATGTATCCAAAA+TGG - chr1_4:11534754-11534773 Msa0150270:five_prime_UTR 35.0%
!! GCCATTTTGGATACATGATA+GGG + chr1_4:11534758-11534777 None:intergenic 35.0%
ACAAATTATGATTGGGCTCT+TGG - chr1_4:11534952-11534971 Msa0150270:five_prime_UTR 35.0%
!! GATTCAAATCTGATTTGGTG+TGG - chr1_4:11534288-11534307 Msa0150270:CDS 35.0%
AAGTTCTGCAAAATGGTGAT+TGG - chr1_4:11534338-11534357 Msa0150270:CDS 35.0%
! AGATCAAGGTGCTTTAATTG+TGG - chr1_4:11534387-11534406 Msa0150270:intron 35.0%
AATAATCCATCCTCTCCATA+AGG + chr1_4:11534449-11534468 None:intergenic 35.0%
TAGGATTAAGTCTCACTTCT+GGG + chr1_4:11534619-11534638 None:intergenic 35.0%
CCCTAAATCTCAATTTGTCT+AGG + chr1_4:11534638-11534657 None:intergenic 35.0%
! GATTTAGGGTGCTTATGAAT+TGG - chr1_4:11534649-11534668 Msa0150270:five_prime_UTR 35.0%
ACCCTATCATGTATCCAAAA+TGG - chr1_4:11534754-11534773 Msa0150270:five_prime_UTR 35.0%
!! GCCATTTTGGATACATGATA+GGG + chr1_4:11534758-11534777 None:intergenic 35.0%
ACAAATTATGATTGGGCTCT+TGG - chr1_4:11534952-11534971 Msa0150270:five_prime_UTR 35.0%
CTAGTACATGCTGAAGATGA+TGG + chr1_4:11534252-11534271 None:intergenic 40.0%
CATCATCTTCAGCATGTACT+AGG - chr1_4:11534250-11534269 Msa0150270:CDS 40.0%
AAGATCTAAGTGTCCTTGGT+GGG - chr1_4:11534410-11534429 Msa0150270:intron 40.0%
CTAGGATTAAGTCTCACTTC+TGG + chr1_4:11534620-11534639 None:intergenic 40.0%
!! GGCCATTTTGGATACATGAT+AGG + chr1_4:11534759-11534778 None:intergenic 40.0%
! TTAGGAAGCTTAGGCCATTT+TGG + chr1_4:11534771-11534790 None:intergenic 40.0%
CTAGTACATGCTGAAGATGA+TGG + chr1_4:11534252-11534271 None:intergenic 40.0%
CATCATCTTCAGCATGTACT+AGG - chr1_4:11534250-11534269 Msa0150270:CDS 40.0%
AAGATCTAAGTGTCCTTGGT+GGG - chr1_4:11534410-11534429 Msa0150270:intron 40.0%
CTAGGATTAAGTCTCACTTC+TGG + chr1_4:11534620-11534639 None:intergenic 40.0%
!! GGCCATTTTGGATACATGAT+AGG + chr1_4:11534759-11534778 None:intergenic 40.0%
! TTAGGAAGCTTAGGCCATTT+TGG + chr1_4:11534771-11534790 None:intergenic 40.0%
GTGGAAGATCTAAGTGTCCT+TGG - chr1_4:11534406-11534425 Msa0150270:intron 45.0%
GAAGATCTAAGTGTCCTTGG+TGG - chr1_4:11534409-11534428 Msa0150270:intron 45.0%
AATGCTGAAAGCCAGACAGT+AGG - chr1_4:11534672-11534691 Msa0150270:five_prime_UTR 45.0%
AGTAGGAGAGTTAGCTGGAT+TGG - chr1_4:11534689-11534708 Msa0150270:five_prime_UTR 45.0%
!! AGTTAGCTGGATTGGTAGCA+GGG - chr1_4:11534697-11534716 Msa0150270:five_prime_UTR 45.0%
GAGAAATTCCCGAGTGAAGT+TGG - chr1_4:11534991-11535010 Msa0150270:five_prime_UTR 45.0%
TGATCTTCGCCAACTTCACT+CGG + chr1_4:11535003-11535022 None:intergenic 45.0%
GTGAAGTTGGCGAAGATCAT+TGG - chr1_4:11535004-11535023 Msa0150270:five_prime_UTR 45.0%
GTGGAAGATCTAAGTGTCCT+TGG - chr1_4:11534406-11534425 Msa0150270:intron 45.0%
GAAGATCTAAGTGTCCTTGG+TGG - chr1_4:11534409-11534428 Msa0150270:intron 45.0%
AATGCTGAAAGCCAGACAGT+AGG - chr1_4:11534672-11534691 Msa0150270:five_prime_UTR 45.0%
AGTAGGAGAGTTAGCTGGAT+TGG - chr1_4:11534689-11534708 Msa0150270:five_prime_UTR 45.0%
!! AGTTAGCTGGATTGGTAGCA+GGG - chr1_4:11534697-11534716 Msa0150270:five_prime_UTR 45.0%
GAGAAATTCCCGAGTGAAGT+TGG - chr1_4:11534991-11535010 Msa0150270:five_prime_UTR 45.0%
TGATCTTCGCCAACTTCACT+CGG + chr1_4:11535003-11535022 None:intergenic 45.0%
GTGAAGTTGGCGAAGATCAT+TGG - chr1_4:11535004-11535023 Msa0150270:five_prime_UTR 45.0%
! CTCTCCATAAGGCATCCCAA+AGG + chr1_4:11534438-11534457 None:intergenic 50.0%
!! CTTTGGGATGCCTTATGGAG+AGG - chr1_4:11534436-11534455 Msa0150270:intron 50.0%
CAGCTAACTCTCCTACTGTC+TGG + chr1_4:11534686-11534705 None:intergenic 50.0%
CAGACAGTAGGAGAGTTAGC+TGG - chr1_4:11534684-11534703 Msa0150270:five_prime_UTR 50.0%
!! GAGTTAGCTGGATTGGTAGC+AGG - chr1_4:11534696-11534715 Msa0150270:five_prime_UTR 50.0%
!! TGGATTGGTAGCAGGGTAGT+TGG - chr1_4:11534704-11534723 Msa0150270:five_prime_UTR 50.0%
! GGATTGGTAGCAGGGTAGTT+GGG - chr1_4:11534705-11534724 Msa0150270:five_prime_UTR 50.0%
GATCTTCGCCAACTTCACTC+GGG + chr1_4:11535002-11535021 None:intergenic 50.0%
ATAGACTGCATCCTGACGCT+TGG - chr1_4:11535043-11535062 Msa0150270:five_prime_UTR 50.0%
! CTCTCCATAAGGCATCCCAA+AGG + chr1_4:11534438-11534457 None:intergenic 50.0%
!! CTTTGGGATGCCTTATGGAG+AGG - chr1_4:11534436-11534455 Msa0150270:intron 50.0%
CAGCTAACTCTCCTACTGTC+TGG + chr1_4:11534686-11534705 None:intergenic 50.0%
CAGACAGTAGGAGAGTTAGC+TGG - chr1_4:11534684-11534703 Msa0150270:five_prime_UTR 50.0%
!! GAGTTAGCTGGATTGGTAGC+AGG - chr1_4:11534696-11534715 Msa0150270:five_prime_UTR 50.0%
!! TGGATTGGTAGCAGGGTAGT+TGG - chr1_4:11534704-11534723 Msa0150270:five_prime_UTR 50.0%
! GGATTGGTAGCAGGGTAGTT+GGG - chr1_4:11534705-11534724 Msa0150270:five_prime_UTR 50.0%
GATCTTCGCCAACTTCACTC+GGG + chr1_4:11535002-11535021 None:intergenic 50.0%
ATAGACTGCATCCTGACGCT+TGG - chr1_4:11535043-11535062 Msa0150270:five_prime_UTR 50.0%
! TGTCCTTGGTGGGTGCCTTT+GGG - chr1_4:11534420-11534439 Msa0150270:intron 55.0%
!! GGTGCCTTTGGGATGCCTTA+TGG - chr1_4:11534431-11534450 Msa0150270:intron 55.0%
GGGATGCCTTATGGAGAGGA+TGG - chr1_4:11534440-11534459 Msa0150270:intron 55.0%
! TGTCCTTGGTGGGTGCCTTT+GGG - chr1_4:11534420-11534439 Msa0150270:intron 55.0%
!! GGTGCCTTTGGGATGCCTTA+TGG - chr1_4:11534431-11534450 Msa0150270:intron 55.0%
GGGATGCCTTATGGAGAGGA+TGG - chr1_4:11534440-11534459 Msa0150270:intron 55.0%
! GTGTCCTTGGTGGGTGCCTT+TGG - chr1_4:11534419-11534438 Msa0150270:intron 60.0%
!! CATCCCAAAGGCACCCACCA+AGG + chr1_4:11534426-11534445 None:intergenic 60.0%
! GTGTCCTTGGTGGGTGCCTT+TGG - chr1_4:11534419-11534438 Msa0150270:intron 60.0%
!! CATCCCAAAGGCACCCACCA+AGG + chr1_4:11534426-11534445 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 11534231 11535068 11534231 ID=Msa0150270;Name=Msa0150270
chr1_4 mRNA 11534231 11535068 11534231 ID=Msa0150270-mRNA-1;Parent=Msa0150270;Name=Msa0150270-mRNA-1;_AED=0.20;_eAED=0.20;_QI=519|1|0.5|1|1|1|2|0|78
chr1_4 exon 11534231 11534389 11534231 ID=Msa0150270-mRNA-1:exon:4802;Parent=Msa0150270-mRNA-1
chr1_4 exon 11534472 11535068 11534472 ID=Msa0150270-mRNA-1:exon:4801;Parent=Msa0150270-mRNA-1
chr1_4 five_prime_UTR 11534550 11535068 11534550 ID=Msa0150270-mRNA-1:five_prime_utr;Parent=Msa0150270-mRNA-1
chr1_4 CDS 11534472 11534549 11534472 ID=Msa0150270-mRNA-1:cds;Parent=Msa0150270-mRNA-1
chr1_4 CDS 11534231 11534389 11534231 ID=Msa0150270-mRNA-1:cds;Parent=Msa0150270-mRNA-1
Gene Sequence

>Msa0150270

ATGTACCCTATCATGTATCCAAAATGGCCTAAGCTTCCTAAAACAAAGAAACACTACATTTATGAGAACAACTTGAAGGTAAAATTTATGGTCAACCATGAAGATCACCAGATATTTAACACAAATTATGATTGGGCTCTTGGCGTTAAGCAAAATATAGAGAAATTCCCGAGTGAAGTTGGCGAAGATCATTGGACCATGTATATTGATTATAGACTGCATCCTGACGCTTGGTAA

Protein sequence

>Msa0150270

MYPIMYPKWPKLPKTKKHYIYENNLKVKFMVNHEDHQIFNTNYDWALGVKQNIEKFPSEVGEDHWTMYIDYRLHPDAW*