AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0857950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0857950 MtrunA17_Chr5g0436201 97.183 213 4 2 1 213 1 211 1.63e-152 421
Msa0857950 MtrunA17_Chr3g0112791 55.963 218 69 5 1 213 22 217 4.49e-69 210
Msa0857950 MtrunA17_Chr7g0240961 53.202 203 87 5 13 213 38 234 4.45e-62 193
Msa0857950 MtrunA17_Chr2g0284111 38.686 137 65 3 87 211 153 282 2.86e-29 110
Msa0857950 MtrunA17_Chr4g0045171 37.778 180 80 5 44 211 139 298 1.68e-28 108
Msa0857950 MtrunA17_Chr8g0379191 37.222 180 81 5 44 211 150 309 3.97e-27 105
Msa0857950 MtrunA17_Chr3g0112811 60.241 83 23 2 59 136 46 123 3.11e-21 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0857950 AT5G05830.1 63.580 162 54 3 54 213 45 203 6.25e-68 207
Msa0857950 AT5G01070.1 53.077 130 58 1 87 213 75 204 4.67e-42 141
Msa0857950 AT2G37950.1 50.769 130 56 3 87 213 81 205 2.49e-39 134
Msa0857950 AT5G59000.1 44.937 158 44 5 89 211 80 229 5.35e-33 119
Msa0857950 AT2G34200.2 44.944 89 38 2 87 168 99 183 3.91e-17 77.0
Msa0857950 AT2G34200.1 44.944 89 38 2 87 168 99 183 3.91e-17 77.0

Find 76 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATCCTTTGATACTATTCT+AGG 0.190372 5_4:+54929661 None:intergenic
CACACAATTCATTAACCTTT+TGG 0.244579 5_4:-54932174 Msa0857950:five_prime_UTR
TGTTGCATCTCTCTCTCTAT+AGG 0.271167 5_4:-54932257 Msa0857950:five_prime_UTR
GAGGCTTGGTTCAAGATTAA+AGG 0.278430 5_4:-54931655 Msa0857950:CDS
TGTCTCTCCATAGTTTGATT+TGG 0.297388 5_4:-54929757 Msa0857950:three_prime_UTR
TAGGATTTGTCATCTTAGTA+TGG 0.302408 5_4:-54931764 Msa0857950:CDS
TTCTGATGTTAAGGTGCATT+TGG 0.316270 5_4:-54931806 Msa0857950:CDS
GAATTTCCTTCTAGCATGTA+TGG 0.346946 5_4:-54929906 Msa0857950:CDS
TTACATGAAGATTCTCATCT+TGG 0.360541 5_4:-54932066 Msa0857950:five_prime_UTR
TACATCTCCAAATCAAACTA+TGG 0.373623 5_4:+54929750 None:intergenic
TTTAGTTTAATGTTGTGTTT+TGG 0.374492 5_4:-54929515 Msa0857950:three_prime_UTR
GATATGACAAATCATGAATC+TGG 0.374563 5_4:-54931742 Msa0857950:CDS
ATAAGGTGGAGAGGGATTGT+AGG 0.391162 5_4:-54931783 Msa0857950:CDS
CCCTCCATTGTACCACCAAC+TGG 0.414352 5_4:-54929980 Msa0857950:CDS
GTTGCATCTCTCTCTCTATA+GGG 0.414991 5_4:-54932256 Msa0857950:five_prime_UTR
TGGTTTAGTTTCTGATGTTA+AGG 0.416319 5_4:-54931815 Msa0857950:CDS
TTTGATCCTCCTCATTTAAC+CGG 0.432004 5_4:+54931941 None:intergenic
ATAAGCATTGTGCTGAGGCT+TGG 0.438291 5_4:-54931669 Msa0857950:CDS
CGAAAACCATACATGCTAGA+AGG 0.444571 5_4:+54929900 None:intergenic
TCTTGGTTGTTTCAAAATGC+AGG 0.445406 5_4:-54932049 Msa0857950:exon
AAACTATCTTCATCAAACTT+TGG 0.447618 5_4:-54932122 Msa0857950:five_prime_UTR
GATCAAAGGTGTGAGATTGT+AGG 0.451686 5_4:-54931925 Msa0857950:CDS
ATGTTCAGTGGAGTTGGATC+TGG 0.459424 5_4:-54931845 Msa0857950:CDS
ACAATACAATTTATACATGA+TGG 0.464179 5_4:+54929580 None:intergenic
TGTATATATAATTAATATTG+AGG 0.468168 5_4:-54929492 Msa0857950:three_prime_UTR
CTATCTTCATCAAACTTTGG+TGG 0.476920 5_4:-54932119 Msa0857950:five_prime_UTR
TTTCAAAATGCAGGTAAATG+AGG 0.481861 5_4:-54932040 Msa0857950:exon
TCATGAATCTGGAATTCCTA+TGG 0.482383 5_4:-54931731 Msa0857950:CDS
ATCAGAATGTTCAGTGGAGT+TGG 0.482682 5_4:-54931851 Msa0857950:CDS
CGAGTTTCTGATGGTGGTGA+AGG 0.510573 5_4:+54929956 None:intergenic
TCTGGAATTCCTATGGAGCT+TGG 0.510778 5_4:-54931724 Msa0857950:CDS
TGTTAAGGTGCATTTGGATA+AGG 0.511468 5_4:-54931800 Msa0857950:CDS
TGAAGCAGGTAACATGATAG+AGG 0.511966 5_4:-54929813 Msa0857950:three_prime_UTR
GAGTTGGATCTGGATGGTGG+TGG 0.518478 5_4:-54931835 Msa0857950:CDS
GCATTTGGATAAGGTGGAGA+GGG 0.524811 5_4:-54931791 Msa0857950:CDS
TAAGGTGCATTTGGATAAGG+TGG 0.527254 5_4:-54931797 Msa0857950:CDS
TCAAACTATGGAGAGACATA+AGG 0.529923 5_4:+54929762 None:intergenic
CTCCTCATTTAACCGGTCAC+CGG 0.540911 5_4:+54931948 None:intergenic
ATATTACTCTACACTGTGGT+AGG 0.547584 5_4:+54932204 None:intergenic
TCCAGTTGGTGGTACAATGG+AGG 0.550684 5_4:+54929979 None:intergenic
CCAGTTGGTGGTACAATGGA+GGG 0.553362 5_4:+54929980 None:intergenic
CATTTGAACTTCCATAGCAC+CGG 0.554330 5_4:+54930030 None:intergenic
GATGTTCTAACTCCGGTGAC+CGG 0.563674 5_4:-54931960 Msa0857950:CDS
GTGTAACTTGAGGATGAAGC+AGG 0.566646 5_4:-54929827 Msa0857950:three_prime_UTR
CTCCGGTGACCGGTTAAATG+AGG 0.569406 5_4:-54931950 Msa0857950:CDS
TCAACCTAGAATAGTATCAA+AGG 0.572905 5_4:-54929665 Msa0857950:three_prime_UTR
ACACAATGTAGCCGGTGCTA+TGG 0.576578 5_4:-54930041 Msa0857950:CDS
TAGAACATCGTTCATTACTC+CGG 0.582687 5_4:+54931974 None:intergenic
TCAATTGCACACAATGTAGC+CGG 0.585989 5_4:-54930049 Msa0857950:CDS
GGTGGTGAAGGTCCAGTTGG+TGG 0.586158 5_4:+54929968 None:intergenic
TTGAATTCCTGTGTAACTTG+AGG 0.588291 5_4:-54929837 Msa0857950:exon
GTTGAAATTGATACACATGT+TGG 0.588752 5_4:-54929559 Msa0857950:three_prime_UTR
TGCTCATAAGCATTGTGCTG+AGG 0.590807 5_4:-54931674 Msa0857950:CDS
TGCATTTGGATAAGGTGGAG+AGG 0.590996 5_4:-54931792 Msa0857950:CDS
GTGGAGTTGGATCTGGATGG+TGG 0.591746 5_4:-54931838 Msa0857950:CDS
GGTTAAATGAGGAGGATCAA+AGG 0.593905 5_4:-54931939 Msa0857950:CDS
TAATTAATATTGAGGAGTGA+AGG 0.594633 5_4:-54929484 None:intergenic
CTTCACCACCATCAGAAACT+CGG 0.596501 5_4:-54929955 Msa0857950:CDS
AAATTCCGAGTTTCTGATGG+TGG 0.598667 5_4:+54929950 None:intergenic
AGGTCCAGTTGGTGGTACAA+TGG 0.605202 5_4:+54929976 None:intergenic
AATGAACGATGTTCTAACTC+CGG 0.609785 5_4:-54931967 Msa0857950:CDS
GATGGTGGTGAAGGTCCAGT+TGG 0.613111 5_4:+54929965 None:intergenic
TAGACAACAATGTATGTCAC+CGG 0.620225 5_4:-54931993 Msa0857950:CDS
AACAGAACTTGTGAAATCTG+TGG 0.623786 5_4:-54930073 Msa0857950:intron
CGGTGACCGGTTAAATGAGG+AGG 0.626754 5_4:-54931947 Msa0857950:CDS
GCTTCATCCTCAAGTTACAC+AGG 0.634243 5_4:+54929830 None:intergenic
GAAGCAGGTAACATGATAGA+GGG 0.636600 5_4:-54929812 Msa0857950:three_prime_UTR
ATCTGAATCAGAATGTTCAG+TGG 0.639058 5_4:-54931857 Msa0857950:CDS
TGATAGAGGGTTATGAACAC+AGG 0.646635 5_4:-54929799 Msa0857950:three_prime_UTR
TGATACACATGTTGGACATG+TGG 0.655246 5_4:-54929551 Msa0857950:three_prime_UTR
AAGTTACACAGGAATTCAAG+AGG 0.657142 5_4:+54929841 None:intergenic
TCAGTGGAGTTGGATCTGGA+TGG 0.667485 5_4:-54931841 Msa0857950:CDS
AGTTACACAGGAATTCAAGA+GGG 0.673281 5_4:+54929842 None:intergenic
TACACATGTTGGACATGTGG+AGG 0.699270 5_4:-54929548 Msa0857950:three_prime_UTR
CAGTTGGTGGTACAATGGAG+GGG 0.714794 5_4:+54929981 None:intergenic
AAAGATATTACTCTACACTG+TGG 0.726392 5_4:+54932200 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATATATAATTAATATTG+AGG - chr5_4:54932272-54932291 Msa0857950:five_prime_UTR 10.0%
!!! AGATAGAAATATTATCTTTT+AGG - chr5_4:54930464-54930483 Msa0857950:intron 15.0%
!! AAATTGAACATATAATTGTA+TGG - chr5_4:54931220-54931239 Msa0857950:intron 15.0%
!! AATATATTGAAATCAAAACT+TGG + chr5_4:54931310-54931329 None:intergenic 15.0%
!!! ATGTTTTTGCAAAAATGATT+TGG - chr5_4:54930063-54930082 Msa0857950:CDS 20.0%
!! TTTACAACTTTATTCCAAAA+GGG + chr5_4:54930158-54930177 None:intergenic 20.0%
!! ATTTACAACTTTATTCCAAA+AGG + chr5_4:54930159-54930178 None:intergenic 20.0%
!! ACATATCAACTAAAATATAG+TGG + chr5_4:54930210-54930229 None:intergenic 20.0%
!! AATGGTTGAAAGAAAAATAT+TGG - chr5_4:54930988-54931007 Msa0857950:intron 20.0%
!! TTTGAGTATAATTTCACTAT+AGG - chr5_4:54931368-54931387 Msa0857950:intron 20.0%
!! AATGTAAAGTGAAATTTGAA+AGG - chr5_4:54931505-54931524 Msa0857950:intron 20.0%
!! CAAGTTAAATTGTTTAATGT+TGG - chr5_4:54931573-54931592 Msa0857950:intron 20.0%
!! ACAATACAATTTATACATGA+TGG + chr5_4:54932187-54932206 None:intergenic 20.0%
!!! TTTAGTTTAATGTTGTGTTT+TGG - chr5_4:54932249-54932268 Msa0857950:five_prime_UTR 20.0%
! AAACTATCTTCATCAAACTT+TGG - chr5_4:54929642-54929661 Msa0857950:three_prime_UTR 25.0%
! ATCTACAATTTCATTCAATG+TGG + chr5_4:54930268-54930287 None:intergenic 25.0%
!! ACAATGTATAAGAGTATGTT+TGG - chr5_4:54930293-54930312 Msa0857950:intron 25.0%
!!! TTTTAGGTAGAATTGATTTG+TGG - chr5_4:54930480-54930499 Msa0857950:intron 25.0%
!! ACTAAACCAAACATGATTTT+GGG - chr5_4:54930563-54930582 Msa0857950:intron 25.0%
! ACAAACATGGAATTAAACAT+CGG - chr5_4:54930590-54930609 Msa0857950:intron 25.0%
! AAACAAAATCTTAAGACATC+CGG + chr5_4:54930636-54930655 None:intergenic 25.0%
!!! TAAGATTTTGTTTGAAGATG+CGG - chr5_4:54930643-54930662 Msa0857950:intron 25.0%
!! CTCTTATGTTTTTCTTTTTG+TGG - chr5_4:54930736-54930755 Msa0857950:intron 25.0%
!! TGTTTTTCTTTTTGTGGATA+AGG - chr5_4:54930742-54930761 Msa0857950:intron 25.0%
! TGAGCTACATTATAATAGAA+AGG - chr5_4:54930954-54930973 Msa0857950:intron 25.0%
! GAGCTACATTATAATAGAAA+GGG - chr5_4:54930955-54930974 Msa0857950:intron 25.0%
! CATTATAATAGAAAGGGTTA+AGG - chr5_4:54930961-54930980 Msa0857950:intron 25.0%
!!! CTGCTTTTATCTTCAATTAT+GGG - chr5_4:54931114-54931133 Msa0857950:intron 25.0%
!!! ATTGAGCTGATTTTTCAATT+TGG + chr5_4:54931156-54931175 None:intergenic 25.0%
!!! GGGATTCTTTTTTCTTTTAT+TGG + chr5_4:54931194-54931213 None:intergenic 25.0%
! AAAAGAAAAAAGAATCCCTT+TGG - chr5_4:54931196-54931215 Msa0857950:intron 25.0%
! AAAGAAAAAAGAATCCCTTT+GGG - chr5_4:54931197-54931216 Msa0857950:intron 25.0%
! TATATGTTCAATTTCCCAAA+GGG + chr5_4:54931214-54931233 None:intergenic 25.0%
! TTATATGTTCAATTTCCCAA+AGG + chr5_4:54931215-54931234 None:intergenic 25.0%
! ATATCTAATGATCAAACTAC+AGG - chr5_4:54931463-54931482 Msa0857950:intron 25.0%
! ACTACAGGAAGATTAATATA+TGG - chr5_4:54931478-54931497 Msa0857950:intron 25.0%
! AGATAGAACTGATTATTGAT+AGG - chr5_4:54931545-54931564 Msa0857950:intron 25.0%
AAAGATATTACTCTACACTG+TGG + chr5_4:54929567-54929586 None:intergenic 30.0%
! CACACAATTCATTAACCTTT+TGG - chr5_4:54929590-54929609 Msa0857950:three_prime_UTR 30.0%
CTCAACAAAAAAGAACCAAA+AGG + chr5_4:54929608-54929627 None:intergenic 30.0%
TTACATGAAGATTCTCATCT+TGG - chr5_4:54929698-54929717 Msa0857950:three_prime_UTR 30.0%
TTTCAAAATGCAGGTAAATG+AGG - chr5_4:54929724-54929743 Msa0857950:three_prime_UTR 30.0%
! TGGTTTAGTTTCTGATGTTA+AGG - chr5_4:54929949-54929968 Msa0857950:CDS 30.0%
TAGGATTTGTCATCTTAGTA+TGG - chr5_4:54930000-54930019 Msa0857950:CDS 30.0%
GATATGACAAATCATGAATC+TGG - chr5_4:54930022-54930041 Msa0857950:CDS 30.0%
AAATCAATTCACTTCTGTGA+CGG + chr5_4:54930324-54930343 None:intergenic 30.0%
! GACTAAACCAAACATGATTT+TGG - chr5_4:54930562-54930581 Msa0857950:intron 30.0%
AATAGCTCAAGATAAGGTAA+AGG + chr5_4:54930775-54930794 None:intergenic 30.0%
AAGTCAAATAGCTCAAGATA+AGG + chr5_4:54930781-54930800 None:intergenic 30.0%
TCATTTACTGCCAAATACTT+TGG - chr5_4:54930829-54930848 Msa0857950:intron 30.0%
! ACCACATTATCCAAAGTATT+TGG + chr5_4:54930842-54930861 None:intergenic 30.0%
ACTATGAAATGTCTTGATAG+TGG - chr5_4:54930873-54930892 Msa0857950:intron 30.0%
AGAAAGGGTTAAGGTAATAA+TGG - chr5_4:54930970-54930989 Msa0857950:intron 30.0%
CCATAATTGAAGATAAAAGC+AGG + chr5_4:54931116-54931135 None:intergenic 30.0%
!! CCTGCTTTTATCTTCAATTA+TGG - chr5_4:54931113-54931132 Msa0857950:intron 30.0%
AAAAAGCCATGATATGACAA+AGG + chr5_4:54931890-54931909 None:intergenic 30.0%
TACATCTCCAAATCAAACTA+TGG + chr5_4:54932017-54932036 None:intergenic 30.0%
TCAACCTAGAATAGTATCAA+AGG - chr5_4:54932099-54932118 Msa0857950:five_prime_UTR 30.0%
ACATCCTTTGATACTATTCT+AGG + chr5_4:54932106-54932125 None:intergenic 30.0%
GTTGAAATTGATACACATGT+TGG - chr5_4:54932205-54932224 Msa0857950:five_prime_UTR 30.0%
ATATTACTCTACACTGTGGT+AGG + chr5_4:54929563-54929582 None:intergenic 35.0%
CTATCTTCATCAAACTTTGG+TGG - chr5_4:54929645-54929664 Msa0857950:three_prime_UTR 35.0%
! TCTTGGTTGTTTCAAAATGC+AGG - chr5_4:54929715-54929734 Msa0857950:three_prime_UTR 35.0%
TAGACAACAATGTATGTCAC+CGG - chr5_4:54929771-54929790 Msa0857950:three_prime_UTR 35.0%
TAGAACATCGTTCATTACTC+CGG + chr5_4:54929793-54929812 None:intergenic 35.0%
AATGAACGATGTTCTAACTC+CGG - chr5_4:54929797-54929816 Msa0857950:three_prime_UTR 35.0%
TTTGATCCTCCTCATTTAAC+CGG + chr5_4:54929826-54929845 None:intergenic 35.0%
! GGAGTGTCTGAAAAACAAAA+AGG - chr5_4:54929860-54929879 Msa0857950:CDS 35.0%
ATCTGAATCAGAATGTTCAG+TGG - chr5_4:54929907-54929926 Msa0857950:CDS 35.0%
TTCTGATGTTAAGGTGCATT+TGG - chr5_4:54929958-54929977 Msa0857950:CDS 35.0%
TGTTAAGGTGCATTTGGATA+AGG - chr5_4:54929964-54929983 Msa0857950:CDS 35.0%
! TCATGAATCTGGAATTCCTA+TGG - chr5_4:54930033-54930052 Msa0857950:CDS 35.0%
! AGTACTAGTATACACCCTTT+TGG - chr5_4:54930141-54930160 Msa0857950:intron 35.0%
GAAGTGAGAGATACATATAC+CGG - chr5_4:54930614-54930633 Msa0857950:intron 35.0%
CATTCTTCATGTCAATTGTG+TGG - chr5_4:54930670-54930689 Msa0857950:intron 35.0%
TGTGGTTGTTCGAAGTATAA+TGG - chr5_4:54930688-54930707 Msa0857950:intron 35.0%
GTGGTTGTTCGAAGTATAAT+GGG - chr5_4:54930689-54930708 Msa0857950:intron 35.0%
AGAGAATCCTAAAACACTCT+AGG + chr5_4:54930720-54930739 None:intergenic 35.0%
GGCAGTAAATGAATATGCTA+AGG + chr5_4:54930821-54930840 None:intergenic 35.0%
GCCAAATACTTTGGATAATG+TGG - chr5_4:54930838-54930857 Msa0857950:intron 35.0%
TTGGGAATTGGGATGTAAAT+GGG + chr5_4:54931031-54931050 None:intergenic 35.0%
TTGAATCCTAGTTGGGAATT+GGG + chr5_4:54931042-54931061 None:intergenic 35.0%
ACTATGCTTGAATCCTAGTT+GGG + chr5_4:54931049-54931068 None:intergenic 35.0%
AACTATGCTTGAATCCTAGT+TGG + chr5_4:54931050-54931069 None:intergenic 35.0%
! CTGCTAAGAAGTATGAAACT+TGG - chr5_4:54931244-54931263 Msa0857950:intron 35.0%
TTTCACTATAGGAGCTACTA+AGG - chr5_4:54931379-54931398 Msa0857950:intron 35.0%
TCATTAGATATACGCGCATT+TGG + chr5_4:54931454-54931473 None:intergenic 35.0%
! GCGAATAAAGTGTCAAACTT+AGG + chr5_4:54931601-54931620 None:intergenic 35.0%
AACAGAACTTGTGAAATCTG+TGG - chr5_4:54931691-54931710 Msa0857950:CDS 35.0%
CAAAAATTCCGAGTTTCTGA+TGG + chr5_4:54931820-54931839 None:intergenic 35.0%
! CATCAGAAACTCGGAATTTT+TGG - chr5_4:54931818-54931837 Msa0857950:CDS 35.0%
GAATTTCCTTCTAGCATGTA+TGG - chr5_4:54931858-54931877 Msa0857950:CDS 35.0%
! TTTTCGCCTTTGTCATATCA+TGG - chr5_4:54931881-54931900 Msa0857950:CDS 35.0%
AGTTACACAGGAATTCAAGA+GGG + chr5_4:54931925-54931944 None:intergenic 35.0%
AAGTTACACAGGAATTCAAG+AGG + chr5_4:54931926-54931945 None:intergenic 35.0%
TTGAATTCCTGTGTAACTTG+AGG - chr5_4:54931927-54931946 Msa0857950:CDS 35.0%
TCAAACTATGGAGAGACATA+AGG + chr5_4:54932005-54932024 None:intergenic 35.0%
TGTCTCTCCATAGTTTGATT+TGG - chr5_4:54932007-54932026 Msa0857950:CDS 35.0%
TGTTGCATCTCTCTCTCTAT+AGG - chr5_4:54929507-54929526 Msa0857950:three_prime_UTR 40.0%
GTTGCATCTCTCTCTCTATA+GGG - chr5_4:54929508-54929527 Msa0857950:three_prime_UTR 40.0%
GGTTAAATGAGGAGGATCAA+AGG - chr5_4:54929825-54929844 Msa0857950:three_prime_UTR 40.0%
! GATCAAAGGTGTGAGATTGT+AGG - chr5_4:54929839-54929858 Msa0857950:exon 40.0%
ATCAGAATGTTCAGTGGAGT+TGG - chr5_4:54929913-54929932 Msa0857950:CDS 40.0%
TAAGGTGCATTTGGATAAGG+TGG - chr5_4:54929967-54929986 Msa0857950:CDS 40.0%
CAAAAACATCCAAGCTCCAT+AGG + chr5_4:54930052-54930071 None:intergenic 40.0%
! GAGGCTTGGTTCAAGATTAA+AGG - chr5_4:54930109-54930128 Msa0857950:intron 40.0%
!! GGTAGAATTGATTTGTGGAC+TGG - chr5_4:54930485-54930504 Msa0857950:intron 40.0%
GGAATCATTTCTGCAACTGA+AGG - chr5_4:54930506-54930525 Msa0857950:intron 40.0%
GTTGCTCCCAAAATCATGTT+TGG + chr5_4:54930572-54930591 None:intergenic 40.0%
!! GATTTTGGGAGCAACAAACA+TGG - chr5_4:54930577-54930596 Msa0857950:intron 40.0%
!! GGATGATCCTAGAGTGTTTT+AGG - chr5_4:54930710-54930729 Msa0857950:intron 40.0%
GTTGGGAATTGGGATGTAAA+TGG + chr5_4:54931032-54931051 None:intergenic 40.0%
TTACATCCCAATTCCCAACT+AGG - chr5_4:54931033-54931052 Msa0857950:intron 40.0%
CTTGAATCCTAGTTGGGAAT+TGG + chr5_4:54931043-54931062 None:intergenic 40.0%
TGTGTTTCGATTACTTCTCG+AGG - chr5_4:54931345-54931364 Msa0857950:intron 40.0%
TCAATTGCACACAATGTAGC+CGG - chr5_4:54931715-54931734 Msa0857950:CDS 40.0%
CATTTGAACTTCCATAGCAC+CGG + chr5_4:54931737-54931756 None:intergenic 40.0%
AAATTCCGAGTTTCTGATGG+TGG + chr5_4:54931817-54931836 None:intergenic 40.0%
!!! AGAAACTCGGAATTTTTGGC+AGG - chr5_4:54931822-54931841 Msa0857950:CDS 40.0%
!!! GAAACTCGGAATTTTTGGCA+GGG - chr5_4:54931823-54931842 Msa0857950:CDS 40.0%
CGAAAACCATACATGCTAGA+AGG + chr5_4:54931867-54931886 None:intergenic 40.0%
TGAAGCAGGTAACATGATAG+AGG - chr5_4:54931951-54931970 Msa0857950:CDS 40.0%
GAAGCAGGTAACATGATAGA+GGG - chr5_4:54931952-54931971 Msa0857950:CDS 40.0%
TGATAGAGGGTTATGAACAC+AGG - chr5_4:54931965-54931984 Msa0857950:CDS 40.0%
TGATACACATGTTGGACATG+TGG - chr5_4:54932213-54932232 Msa0857950:five_prime_UTR 40.0%
ATGTTCAGTGGAGTTGGATC+TGG - chr5_4:54929919-54929938 Msa0857950:CDS 45.0%
TGCATTTGGATAAGGTGGAG+AGG - chr5_4:54929972-54929991 Msa0857950:CDS 45.0%
GCATTTGGATAAGGTGGAGA+GGG - chr5_4:54929973-54929992 Msa0857950:CDS 45.0%
ATAAGGTGGAGAGGGATTGT+AGG - chr5_4:54929981-54930000 Msa0857950:CDS 45.0%
! TCTGGAATTCCTATGGAGCT+TGG - chr5_4:54930040-54930059 Msa0857950:CDS 45.0%
! TGCTCATAAGCATTGTGCTG+AGG - chr5_4:54930090-54930109 Msa0857950:intron 45.0%
! ATAAGCATTGTGCTGAGGCT+TGG - chr5_4:54930095-54930114 Msa0857950:intron 45.0%
CTTCACCACCATCAGAAACT+CGG - chr5_4:54931809-54931828 Msa0857950:CDS 45.0%
!!! GGAATTTTTGGCAGGGACAT+CGG - chr5_4:54931830-54931849 Msa0857950:CDS 45.0%
GCTTCATCCTCAAGTTACAC+AGG + chr5_4:54931937-54931956 None:intergenic 45.0%
GTGTAACTTGAGGATGAAGC+AGG - chr5_4:54931937-54931956 Msa0857950:CDS 45.0%
TACACATGTTGGACATGTGG+AGG - chr5_4:54932216-54932235 Msa0857950:five_prime_UTR 45.0%
GATGTTCTAACTCCGGTGAC+CGG - chr5_4:54929804-54929823 Msa0857950:three_prime_UTR 50.0%
CTCCTCATTTAACCGGTCAC+CGG + chr5_4:54929819-54929838 None:intergenic 50.0%
TCAGTGGAGTTGGATCTGGA+TGG - chr5_4:54929923-54929942 Msa0857950:CDS 50.0%
! ACACAATGTAGCCGGTGCTA+TGG - chr5_4:54931723-54931742 Msa0857950:CDS 50.0%
!! CAGTTGGTGGTACAATGGAG+GGG + chr5_4:54931786-54931805 None:intergenic 50.0%
!! CCAGTTGGTGGTACAATGGA+GGG + chr5_4:54931787-54931806 None:intergenic 50.0%
!! TCCAGTTGGTGGTACAATGG+AGG + chr5_4:54931788-54931807 None:intergenic 50.0%
!! AGGTCCAGTTGGTGGTACAA+TGG + chr5_4:54931791-54931810 None:intergenic 50.0%
!! CGAGTTTCTGATGGTGGTGA+AGG + chr5_4:54931811-54931830 None:intergenic 50.0%
CTCCGGTGACCGGTTAAATG+AGG - chr5_4:54929814-54929833 Msa0857950:three_prime_UTR 55.0%
CGGTGACCGGTTAAATGAGG+AGG - chr5_4:54929817-54929836 Msa0857950:three_prime_UTR 55.0%
GTGGAGTTGGATCTGGATGG+TGG - chr5_4:54929926-54929945 Msa0857950:CDS 55.0%
! GAGTTGGATCTGGATGGTGG+TGG - chr5_4:54929929-54929948 Msa0857950:CDS 55.0%
CCCTCCATTGTACCACCAAC+TGG - chr5_4:54931784-54931803 Msa0857950:CDS 55.0%
! GATGGTGGTGAAGGTCCAGT+TGG + chr5_4:54931802-54931821 None:intergenic 55.0%
GGTGGTGAAGGTCCAGTTGG+TGG + chr5_4:54931799-54931818 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 54929485 54932301 54929485 ID=Msa0857950;Name=Msa0857950
chr5_4 mRNA 54929485 54932301 54929485 ID=Msa0857950-mRNA-1;Parent=Msa0857950;Name=Msa0857950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=246|1|1|1|1|1|2|371|213
chr5_4 exon 54929485 54930090 54929485 ID=Msa0857950-mRNA-1:exon:16850;Parent=Msa0857950-mRNA-1
chr5_4 exon 54931649 54932301 54931649 ID=Msa0857950-mRNA-1:exon:16849;Parent=Msa0857950-mRNA-1
chr5_4 five_prime_UTR 54932056 54932301 54932056 ID=Msa0857950-mRNA-1:five_prime_utr;Parent=Msa0857950-mRNA-1
chr5_4 CDS 54931649 54932055 54931649 ID=Msa0857950-mRNA-1:cds;Parent=Msa0857950-mRNA-1
chr5_4 CDS 54929856 54930090 54929856 ID=Msa0857950-mRNA-1:cds;Parent=Msa0857950-mRNA-1
chr5_4 three_prime_UTR 54929485 54929855 54929485 ID=Msa0857950-mRNA-1:three_prime_utr;Parent=Msa0857950-mRNA-1
Gene Sequence

>Msa0857950

ATGCAGGTAAATGAGGACAAGTTTCATGTTGTTGTAGACATAGACAACAATGTATGTCACCGGAGTAATGAACGATGTTCTAACTCCGGTGACCGGTTAAATGAGGAGGATCAAAGGTGTGAGATTGTAGGAGTGTCTGAAAAACAAAAAGGATCTTGTGATTCAGATGATAATTCATCTGAATCAGAATGTTCAGTGGAGTTGGATCTGGATGGTGGTGGTTTAGTTTCTGATGTTAAGGTGCATTTGGATAAGGTGGAGAGGGATTGTAGGATTTGTCATCTTAGTATGGATATGACAAATCATGAATCTGGAATTCCTATGGAGCTTGGATGTTTTTGCAAAAATGATTTGGCTGCTGCTCATAAGCATTGTGCTGAGGCTTGGTTCAAGATTAAAGGCAACAAAACTTGTGAAATCTGTGGATCAATTGCACACAATGTAGCCGGTGCTATGGAAGTTCAAATGACAGAACAATTTAATGAATCAAGTGACCCCTCCATTGTACCACCAACTGGACCTTCACCACCATCAGAAACTCGGAATTTTTGGCAGGGACATCGGTTTTTGAATTTCCTTCTAGCATGTATGGTTTTCGCCTTTGTCATATCATGGCTTTTTCACTTTAATGTTCCCTCTTGA
Protein sequence

>Msa0857950

MQVNEDKFHVVVDIDNNVCHRSNERCSNSGDRLNEEDQRCEIVGVSEKQKGSCDSDDNSSESECSVELDLDGGGLVSDVKVHLDKVERDCRICHLSMDMTNHESGIPMELGCFCKNDLAAAHKHCAEAWFKIKGNKTCEICGSIAHNVAGAMEVQMTEQFNESSDPSIVPPTGPSPPSETRNFWQGHRFLNFLLACMVFAFVISWLFHFNVPS*