Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0884150 | AES61431.2 | 77.193 | 171 | 7 | 1 | 1 | 139 | 81 | 251 | 3.48e-86 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0884150 | G7I8T1 | 77.193 | 171 | 7 | 1 | 1 | 139 | 81 | 251 | 1.66e-86 | 263 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0884150 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0028480 | Msa0884150 | 0.801845 | 7.429389e-49 | -8.615850e-47 |
| Msa0703120 | Msa0884150 | 0.835524 | 1.595954e-56 | -8.615850e-47 |
| Msa0726140 | Msa0884150 | 0.824562 | 7.547485e-54 | -8.615850e-47 |
| Msa0741990 | Msa0884150 | 0.802792 | 4.742067e-49 | -8.615850e-47 |
| Msa0743810 | Msa0884150 | 0.817425 | 3.319899e-52 | -8.615850e-47 |
| Msa0745060 | Msa0884150 | 0.815170 | 1.060147e-51 | -8.615850e-47 |
| Msa0783160 | Msa0884150 | 0.809496 | 1.838440e-50 | -8.615850e-47 |
| Msa0825860 | Msa0884150 | 0.813478 | 2.508133e-51 | -8.615850e-47 |
| Msa0124100 | Msa0884150 | 0.815027 | 1.141013e-51 | -8.615850e-47 |
| Msa0153910 | Msa0884150 | 0.838037 | 3.647483e-57 | -8.615850e-47 |
| Msa0384800 | Msa0884150 | 0.811998 | 5.288914e-51 | -8.615850e-47 |
| Msa0452900 | Msa0884150 | 0.809881 | 1.519926e-50 | -8.615850e-47 |
| Msa0453120 | Msa0884150 | 0.805272 | 1.446258e-49 | -8.615850e-47 |
| Msa0326200 | Msa0884150 | 0.804884 | 1.743691e-49 | -8.615850e-47 |
| Msa0342240 | Msa0884150 | 0.812279 | 4.593151e-51 | -8.615850e-47 |
| Msa0564890 | Msa0884150 | 0.801370 | 9.298230e-49 | -8.615850e-47 |
| Msa0846350 | Msa0884150 | 0.801555 | 8.522019e-49 | -8.615850e-47 |
| Msa0884150 | Msa0885280 | 0.814080 | 1.848712e-51 | -8.615850e-47 |
| Msa0884150 | Msa0885290 | 0.827861 | 1.238241e-54 | -8.615850e-47 |
| Msa0884150 | Msa0907260 | 0.824886 | 6.330154e-54 | -8.615850e-47 |
| Msa0884150 | Msa0956270 | 0.831027 | 2.106497e-55 | -8.615850e-47 |
| Msa0884150 | Msa0986360 | 0.828427 | 9.044771e-55 | -8.615850e-47 |
| Msa0884150 | Msa0989060 | 0.800397 | 1.469064e-48 | -8.615850e-47 |
| Msa0884150 | Msa1016280 | 0.832264 | 1.043921e-55 | -8.615850e-47 |
| Msa0884150 | Msa1025360 | 0.802840 | 4.634042e-49 | -8.615850e-47 |
| Msa0884150 | Msa1044840 | 0.805103 | 1.569198e-49 | -8.615850e-47 |
| Msa0884150 | Msa1060840 | 0.824525 | 7.700568e-54 | -8.615850e-47 |
| Msa0884150 | Msa1092990 | 0.812015 | 5.243736e-51 | -8.615850e-47 |
| Msa0884150 | Msa1100980 | 0.809008 | 2.339713e-50 | -8.615850e-47 |
| Msa0884150 | Msa1109440 | 0.811196 | 7.899586e-51 | -8.615850e-47 |
| Msa0884150 | Msa1136090 | 0.814359 | 1.603971e-51 | -8.615850e-47 |
| Msa0884150 | Msa1149360 | 0.804291 | 2.318215e-49 | -8.615850e-47 |
| Msa0884150 | Msa1150370 | 0.809919 | 1.491178e-50 | -8.615850e-47 |
| Msa0884150 | Msa1182090 | 0.827575 | 1.450147e-54 | -8.615850e-47 |
| Msa0884150 | Msa1187940 | 0.801518 | 8.668550e-49 | -8.615850e-47 |
| Msa0884150 | Msa1192640 | 0.803168 | 3.965178e-49 | -8.615850e-47 |
| Msa0884150 | Msa1230430 | 0.827024 | 1.965384e-54 | -8.615850e-47 |
| Msa0884150 | Msa1269670 | 0.810836 | 9.456963e-51 | -8.615850e-47 |
| Msa0884150 | Msa1270230 | 0.811579 | 6.522523e-51 | -8.615850e-47 |
| Msa0884150 | Msa1273230 | 0.810460 | 1.140302e-50 | -8.615850e-47 |
| Msa0884150 | Msa1283330 | 0.812964 | 3.253074e-51 | -8.615850e-47 |
| Msa0884150 | Msa1285930 | 0.812177 | 4.833095e-51 | -8.615850e-47 |
| Msa0884150 | Msa1287810 | 0.811248 | 7.697154e-51 | -8.615850e-47 |
| Msa0884150 | Msa1402790 | 0.800997 | 1.108167e-48 | -8.615850e-47 |
| Msa0884150 | Msa1404000 | 0.813141 | 2.974578e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0884150 | MtrunA17_Chr6g0475591 | 93.750 | 48 | 3 | 0 | 92 | 139 | 1 | 48 | 6.16e-27 | 96.3 |
| Msa0884150 | MtrunA17_Chr1g0172971 | 88.462 | 52 | 6 | 0 | 1 | 52 | 66 | 117 | 2.09e-24 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGGCCTGTTGTT+AGG | 0.302249 | 6_1:-37084088 | None:intergenic |
| AGAATAATCTAGTTGCATAA+TGG | 0.380344 | 6_1:-37084499 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 6_1:+37084354 | Msa0884150:CDS |
| GAATAATCTAGTTGCATAAT+GGG | 0.397504 | 6_1:-37084498 | None:intergenic |
| ATGTCTATAAGATATATAGA+TGG | 0.423693 | 6_1:+37084209 | Msa0884150:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 6_1:+37084367 | Msa0884150:CDS |
| GAAAGGGTTGGTTGTCAAGC+TGG | 0.440849 | 6_1:+37084157 | Msa0884150:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 6_1:+37084435 | Msa0884150:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 6_1:+37084355 | Msa0884150:CDS |
| GTCTGCTTAATCCTAACAAC+AGG | 0.466827 | 6_1:+37084077 | Msa0884150:CDS |
| TGACAACCAACCCTTTCCTC+AGG | 0.488705 | 6_1:-37084151 | None:intergenic |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 6_1:+37084040 | Msa0884150:CDS |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 6_1:+37084051 | Msa0884150:CDS |
| TGTCTATAAGATATATAGAT+GGG | 0.523615 | 6_1:+37084210 | Msa0884150:CDS |
| TTTAAGTCCGAAAACCGAAA+AGG | 0.524077 | 6_1:-37084406 | None:intergenic |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 6_1:+37084006 | Msa0884150:CDS |
| TTCACAAACTCAATAACCAC+TGG | 0.531770 | 6_1:-37084115 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 6_1:+37084052 | Msa0884150:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 6_1:+37084441 | Msa0884150:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 6_1:+37084216 | Msa0884150:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 6_1:+37084370 | Msa0884150:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 6_1:+37084361 | Msa0884150:CDS |
| ACCACTGGACTGTCTGATGT+TGG | 0.589854 | 6_1:-37084100 | None:intergenic |
| AATTCGTCAAAAGGAGATGG+TGG | 0.616560 | 6_1:+37084444 | Msa0884150:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 6_1:+37084375 | Msa0884150:CDS |
| GCCAACATCAGACAGTCCAG+TGG | 0.711751 | 6_1:+37084099 | Msa0884150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr6_1:37084006-37084025 | Msa0884150:CDS | 20.0% |
| !! | ATGTCTATAAGATATATAGA+TGG | + | chr6_1:37084209-37084228 | Msa0884150:CDS | 20.0% |
| !! | TGTCTATAAGATATATAGAT+GGG | + | chr6_1:37084210-37084229 | Msa0884150:CDS | 20.0% |
| !! | AAATGCTCTAGTTTATGTAA+TGG | - | chr6_1:37084253-37084272 | None:intergenic | 25.0% |
| !!! | GCTTAAATAAATTCTAAAGC+TGG | + | chr6_1:37084322-37084341 | Msa0884150:intron | 25.0% |
| CGTTATGTTTGTAACAAACA+AGG | + | chr6_1:37084040-37084059 | Msa0884150:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr6_1:37084051-37084070 | Msa0884150:CDS | 30.0% | |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr6_1:37084052-37084071 | Msa0884150:CDS | 30.0% | |
| !!! | AGCTGGAATATTTTTGAAGT+TGG | + | chr6_1:37084174-37084193 | Msa0884150:CDS | 30.0% |
| TAAGATATATAGATGGGATG+TGG | + | chr6_1:37084216-37084235 | Msa0884150:CDS | 30.0% | |
| !! | TTTATGTAATGGATGACAGT+GGG | - | chr6_1:37084242-37084261 | None:intergenic | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | + | chr6_1:37084355-37084374 | Msa0884150:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | + | chr6_1:37084435-37084454 | Msa0884150:CDS | 30.0% | |
| TTCACAAACTCAATAACCAC+TGG | - | chr6_1:37084118-37084137 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | chr6_1:37084135-37084154 | Msa0884150:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | chr6_1:37084140-37084159 | Msa0884150:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | chr6_1:37084141-37084160 | Msa0884150:CDS | 35.0% | |
| !! | GTTTATGTAATGGATGACAG+TGG | - | chr6_1:37084243-37084262 | None:intergenic | 35.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr6_1:37084292-37084311 | Msa0884150:intron | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | chr6_1:37084354-37084373 | Msa0884150:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | chr6_1:37084361-37084380 | Msa0884150:CDS | 35.0% | |
| ! | GAATCTTCCTTTTCGGTTTT+CGG | + | chr6_1:37084399-37084418 | Msa0884150:CDS | 35.0% |
| TTTAAGTCCGAAAACCGAAA+AGG | - | chr6_1:37084409-37084428 | None:intergenic | 35.0% | |
| GTCTGCTTAATCCTAACAAC+AGG | + | chr6_1:37084077-37084096 | Msa0884150:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | + | chr6_1:37084441-37084460 | Msa0884150:CDS | 40.0% | |
| AATTCGTCAAAAGGAGATGG+TGG | + | chr6_1:37084444-37084463 | Msa0884150:CDS | 40.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | - | chr6_1:37084091-37084110 | None:intergenic | 45.0% |
| GAAAAACCTGAGGAAAGGGT+TGG | + | chr6_1:37084145-37084164 | Msa0884150:CDS | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr6_1:37084367-37084386 | Msa0884150:CDS | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | + | chr6_1:37084392-37084411 | Msa0884150:CDS | 45.0% |
| ACCACTGGACTGTCTGATGT+TGG | - | chr6_1:37084103-37084122 | None:intergenic | 50.0% | |
| TGACAACCAACCCTTTCCTC+AGG | - | chr6_1:37084154-37084173 | None:intergenic | 50.0% | |
| !! | GAAAGGGTTGGTTGTCAAGC+TGG | + | chr6_1:37084157-37084176 | Msa0884150:CDS | 50.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr6_1:37084370-37084389 | Msa0884150:CDS | 50.0% | |
| GCCAACATCAGACAGTCCAG+TGG | + | chr6_1:37084099-37084118 | Msa0884150:CDS | 55.0% | |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr6_1:37084375-37084394 | Msa0884150:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 37083983 | 37084513 | 37083983 | ID=Msa0884150;Name=Msa0884150 |
| chr6_1 | mRNA | 37083983 | 37084513 | 37083983 | ID=Msa0884150-mRNA-1;Parent=Msa0884150;Name=Msa0884150-mRNA-1;_AED=0.15;_eAED=0.16;_QI=0|0|0|1|1|1|2|0|144 |
| chr6_1 | exon | 37083983 | 37084244 | 37083983 | ID=Msa0884150-mRNA-1:exon:8714;Parent=Msa0884150-mRNA-1 |
| chr6_1 | exon | 37084341 | 37084513 | 37084341 | ID=Msa0884150-mRNA-1:exon:8715;Parent=Msa0884150-mRNA-1 |
| chr6_1 | CDS | 37083983 | 37084244 | 37083983 | ID=Msa0884150-mRNA-1:cds;Parent=Msa0884150-mRNA-1 |
| chr6_1 | CDS | 37084341 | 37084513 | 37084341 | ID=Msa0884150-mRNA-1:cds;Parent=Msa0884150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
