AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0986360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0986360 MtrunA17_Chr4g0058791 82.692 52 9 0 3 54 143 194 2.40e-25 94.7
Msa0986360 MtrunA17_Chr4g0058811 78.000 50 11 0 5 54 147 196 3.83e-22 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0986360 AT5G63060.2 62.500 48 18 0 7 54 115 162 1.32e-16 72.0
Msa0986360 AT5G63060.1 62.500 48 18 0 7 54 150 197 1.44e-16 72.4

Find 22 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTAAATACTTGAATTTCT+TGG 0.237378 6_4:+34552350 Msa0986360:CDS
ATGAAACTTTGCCACCAATT+TGG 0.294300 6_4:-34552997 None:intergenic
GTTGCAATATTTGATCTTAG+AGG 0.304567 6_4:+34552306 Msa0986360:CDS
ATGAAACTTTGCCACCAAAT+TGG 0.332012 6_4:+34552983 Msa0986360:CDS
GAGCAAAATTTAACCTGTAA+AGG 0.455636 6_4:+34553052 Msa0986360:CDS
AATATTTATGACACAGGAAC+AGG 0.462633 6_4:+34552203 None:intergenic
AAACTTTGCCACCAATTTGG+TGG 0.520792 6_4:-34552994 None:intergenic
AAACTTTGCCACCAAATTGG+TGG 0.527989 6_4:+34552986 Msa0986360:CDS
GCATTGAGTAAGCTTCCCAC+TGG 0.533695 6_4:+34552270 Msa0986360:CDS
CACACCCTTTGATCATCTGC+GGG 0.538520 6_4:-34552228 None:intergenic
GTGTGCTTTCTTAATTGAGA+AGG 0.540417 6_4:+34552248 Msa0986360:CDS
AGGTCCCCGCAGATGATCAA+AGG 0.541232 6_4:+34552223 Msa0986360:CDS
CATTGAGTAAGCTTCCCACT+GGG 0.565906 6_4:+34552271 Msa0986360:CDS
ACTATTTGCTCTTCCCCAGT+GGG 0.568043 6_4:-34552285 None:intergenic
CTCTGCAGAAACTCTCAAGA+AGG 0.568915 6_4:+34552948 Msa0986360:intron
CTCTGCAGAAACTCTCAAGA+AGG 0.568915 6_4:-34553032 None:intergenic
GGTCCCCGCAGATGATCAAA+GGG 0.578158 6_4:+34552224 Msa0986360:CDS
AACTATTTGCTCTTCCCCAG+TGG 0.588881 6_4:-34552286 None:intergenic
AAGATGAATATTTATGACAC+AGG 0.624088 6_4:+34552197 None:intergenic
ACACACCCTTTGATCATCTG+CGG 0.643216 6_4:-34552229 None:intergenic
ATTGAGTAAGCTTCCCACTG+GGG 0.730583 6_4:+34552272 Msa0986360:CDS
ACACCCTTTGATCATCTGCG+GGG 0.744212 6_4:-34552227 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTATCAACTAAAAATATT+TGG - chr6_4:34552399-34552418 None:intergenic 15.0%
!! ACTTATCAACTAAAAATATT+TGG - chr6_4:34552399-34552418 None:intergenic 15.0%
!! TCTTAAATACTTGAATTTCT+TGG + chr6_4:34552350-34552369 Msa0986360:CDS 20.0%
!! TTTGTTCAAGATTAGAAAAT+AGG - chr6_4:34552436-34552455 None:intergenic 20.0%
!!! CAAATACATTTCTTTACTTT+TGG + chr6_4:34552468-34552487 Msa0986360:intron 20.0%
!! AAAAAAACAAAAGATCATGT+AGG + chr6_4:34552861-34552880 Msa0986360:intron 20.0%
!! TCTTAAATACTTGAATTTCT+TGG + chr6_4:34552350-34552369 Msa0986360:CDS 20.0%
!! TTTGTTCAAGATTAGAAAAT+AGG - chr6_4:34552436-34552455 None:intergenic 20.0%
!!! CAAATACATTTCTTTACTTT+TGG + chr6_4:34552468-34552487 Msa0986360:intron 20.0%
!! AAAAAAACAAAAGATCATGT+AGG + chr6_4:34552861-34552880 Msa0986360:intron 20.0%
!!! TATTTAAGATCAGCATTTTC+TGG - chr6_4:34552339-34552358 None:intergenic 25.0%
! TTTCTAATCTTGAACAAAAG+TGG + chr6_4:34552438-34552457 Msa0986360:intron 25.0%
! ATTGTCTCAAGTATTATTTG+TGG + chr6_4:34552526-34552545 Msa0986360:intron 25.0%
! GTATGAATGATAAAAATGTC+AGG - chr6_4:34552810-34552829 None:intergenic 25.0%
! ATGTGAAAAGAATGTTATAG+TGG - chr6_4:34552832-34552851 None:intergenic 25.0%
!!! TATTTAAGATCAGCATTTTC+TGG - chr6_4:34552339-34552358 None:intergenic 25.0%
! TTTCTAATCTTGAACAAAAG+TGG + chr6_4:34552438-34552457 Msa0986360:intron 25.0%
! ATTGTCTCAAGTATTATTTG+TGG + chr6_4:34552526-34552545 Msa0986360:intron 25.0%
! GTATGAATGATAAAAATGTC+AGG - chr6_4:34552810-34552829 None:intergenic 25.0%
! ATGTGAAAAGAATGTTATAG+TGG - chr6_4:34552832-34552851 None:intergenic 25.0%
GTTGCAATATTTGATCTTAG+AGG + chr6_4:34552306-34552325 Msa0986360:CDS 30.0%
AATACTTGAGACAATCGATT+GGG - chr6_4:34552521-34552540 None:intergenic 30.0%
TAATACTTGAGACAATCGAT+TGG - chr6_4:34552522-34552541 None:intergenic 30.0%
GTTAAAATCACATGCTTCTT+AGG + chr6_4:34552595-34552614 Msa0986360:intron 30.0%
GTGAAGTAATAAAGAGATTG+AGG - chr6_4:34552709-34552728 None:intergenic 30.0%
CCAAGATAACACATTAATCA+GGG - chr6_4:34552772-34552791 None:intergenic 30.0%
ACCAAGATAACACATTAATC+AGG - chr6_4:34552773-34552792 None:intergenic 30.0%
TGATTAATGTGTTATCTTGG+TGG + chr6_4:34552772-34552791 Msa0986360:intron 30.0%
GATTAATGTGTTATCTTGGT+GGG + chr6_4:34552773-34552792 Msa0986360:intron 30.0%
CCTGTAAAGGAAAATGAAAA+TGG - chr6_4:34552918-34552937 None:intergenic 30.0%
! CCATTTTCATTTTCCTTTAC+AGG + chr6_4:34552915-34552934 Msa0986360:intron 30.0%
GAGCAAAATTTAACCTGTAA+AGG - chr6_4:34553052-34553071 None:intergenic 30.0%
GAGCAAAATTTAACCTGTAA+AGG + chr6_4:34553052-34553071 Msa0986360:CDS 30.0%
GTTGCAATATTTGATCTTAG+AGG + chr6_4:34552306-34552325 Msa0986360:CDS 30.0%
AATACTTGAGACAATCGATT+GGG - chr6_4:34552521-34552540 None:intergenic 30.0%
TAATACTTGAGACAATCGAT+TGG - chr6_4:34552522-34552541 None:intergenic 30.0%
GTTAAAATCACATGCTTCTT+AGG + chr6_4:34552595-34552614 Msa0986360:intron 30.0%
GTGAAGTAATAAAGAGATTG+AGG - chr6_4:34552709-34552728 None:intergenic 30.0%
CCAAGATAACACATTAATCA+GGG - chr6_4:34552772-34552791 None:intergenic 30.0%
ACCAAGATAACACATTAATC+AGG - chr6_4:34552773-34552792 None:intergenic 30.0%
TGATTAATGTGTTATCTTGG+TGG + chr6_4:34552772-34552791 Msa0986360:intron 30.0%
GATTAATGTGTTATCTTGGT+GGG + chr6_4:34552773-34552792 Msa0986360:intron 30.0%
CCTGTAAAGGAAAATGAAAA+TGG - chr6_4:34552918-34552937 None:intergenic 30.0%
! CCATTTTCATTTTCCTTTAC+AGG + chr6_4:34552915-34552934 Msa0986360:intron 30.0%
GAGCAAAATTTAACCTGTAA+AGG - chr6_4:34553052-34553071 None:intergenic 30.0%
GAGCAAAATTTAACCTGTAA+AGG + chr6_4:34553052-34553071 Msa0986360:CDS 30.0%
GTGTGCTTTCTTAATTGAGA+AGG + chr6_4:34552248-34552267 Msa0986360:CDS 35.0%
TGTCTGTGTATAGTATGCTA+AGG + chr6_4:34552670-34552689 Msa0986360:intron 35.0%
GTCTGTGTATAGTATGCTAA+GGG + chr6_4:34552671-34552690 Msa0986360:intron 35.0%
TCAGGGATATAAACTTGACA+TGG - chr6_4:34552755-34552774 None:intergenic 35.0%
CCCTGATTAATGTGTTATCT+TGG + chr6_4:34552769-34552788 Msa0986360:intron 35.0%
!! TTAGCCTTGTTTGACATGAT+TGG + chr6_4:34552891-34552910 Msa0986360:intron 35.0%
ATGAAACTTTGCCACCAAAT+TGG + chr6_4:34552983-34553002 Msa0986360:CDS 35.0%
ATGAAACTTTGCCACCAATT+TGG - chr6_4:34553000-34553019 None:intergenic 35.0%
GTGTGCTTTCTTAATTGAGA+AGG + chr6_4:34552248-34552267 Msa0986360:CDS 35.0%
TGTCTGTGTATAGTATGCTA+AGG + chr6_4:34552670-34552689 Msa0986360:intron 35.0%
GTCTGTGTATAGTATGCTAA+GGG + chr6_4:34552671-34552690 Msa0986360:intron 35.0%
TCAGGGATATAAACTTGACA+TGG - chr6_4:34552755-34552774 None:intergenic 35.0%
CCCTGATTAATGTGTTATCT+TGG + chr6_4:34552769-34552788 Msa0986360:intron 35.0%
!! TTAGCCTTGTTTGACATGAT+TGG + chr6_4:34552891-34552910 Msa0986360:intron 35.0%
ATGAAACTTTGCCACCAAAT+TGG + chr6_4:34552983-34553002 Msa0986360:CDS 35.0%
ATGAAACTTTGCCACCAATT+TGG - chr6_4:34553000-34553019 None:intergenic 35.0%
TACAATGGCTTAAACACAGC+AGG - chr6_4:34552557-34552576 None:intergenic 40.0%
CTGTGTTTAAGCCATTGTAG+CGG + chr6_4:34552558-34552577 Msa0986360:intron 40.0%
TTCTTCATAAGCCGCTACAA+TGG - chr6_4:34552572-34552591 None:intergenic 40.0%
TGTGTATAGTATGCTAAGGG+TGG + chr6_4:34552674-34552693 Msa0986360:intron 40.0%
TGGACCAATCATGTCAAACA+AGG - chr6_4:34552898-34552917 None:intergenic 40.0%
AAACTTTGCCACCAAATTGG+TGG + chr6_4:34552986-34553005 Msa0986360:CDS 40.0%
AAACTTTGCCACCAATTTGG+TGG - chr6_4:34552997-34553016 None:intergenic 40.0%
TACAATGGCTTAAACACAGC+AGG - chr6_4:34552557-34552576 None:intergenic 40.0%
CTGTGTTTAAGCCATTGTAG+CGG + chr6_4:34552558-34552577 Msa0986360:intron 40.0%
TTCTTCATAAGCCGCTACAA+TGG - chr6_4:34552572-34552591 None:intergenic 40.0%
TGTGTATAGTATGCTAAGGG+TGG + chr6_4:34552674-34552693 Msa0986360:intron 40.0%
TGGACCAATCATGTCAAACA+AGG - chr6_4:34552898-34552917 None:intergenic 40.0%
AAACTTTGCCACCAAATTGG+TGG + chr6_4:34552986-34553005 Msa0986360:CDS 40.0%
AAACTTTGCCACCAATTTGG+TGG - chr6_4:34552997-34553016 None:intergenic 40.0%
ACACACCCTTTGATCATCTG+CGG - chr6_4:34552232-34552251 None:intergenic 45.0%
CATTGAGTAAGCTTCCCACT+GGG + chr6_4:34552271-34552290 Msa0986360:CDS 45.0%
ATTGAGTAAGCTTCCCACTG+GGG + chr6_4:34552272-34552291 Msa0986360:CDS 45.0%
ACTATTTGCTCTTCCCCAGT+GGG - chr6_4:34552288-34552307 None:intergenic 45.0%
AACTATTTGCTCTTCCCCAG+TGG - chr6_4:34552289-34552308 None:intergenic 45.0%
CTCTGCAGAAACTCTCAAGA+AGG + chr6_4:34552948-34552967 Msa0986360:intron 45.0%
CTCTGCAGAAACTCTCAAGA+AGG - chr6_4:34552948-34552967 None:intergenic 45.0%
ACACACCCTTTGATCATCTG+CGG - chr6_4:34552232-34552251 None:intergenic 45.0%
CATTGAGTAAGCTTCCCACT+GGG + chr6_4:34552271-34552290 Msa0986360:CDS 45.0%
ATTGAGTAAGCTTCCCACTG+GGG + chr6_4:34552272-34552291 Msa0986360:CDS 45.0%
ACTATTTGCTCTTCCCCAGT+GGG - chr6_4:34552288-34552307 None:intergenic 45.0%
AACTATTTGCTCTTCCCCAG+TGG - chr6_4:34552289-34552308 None:intergenic 45.0%
CTCTGCAGAAACTCTCAAGA+AGG + chr6_4:34552948-34552967 Msa0986360:intron 45.0%
CTCTGCAGAAACTCTCAAGA+AGG - chr6_4:34552948-34552967 None:intergenic 45.0%
ACACCCTTTGATCATCTGCG+GGG - chr6_4:34552230-34552249 None:intergenic 50.0%
CACACCCTTTGATCATCTGC+GGG - chr6_4:34552231-34552250 None:intergenic 50.0%
GCATTGAGTAAGCTTCCCAC+TGG + chr6_4:34552270-34552289 Msa0986360:CDS 50.0%
ACACCCTTTGATCATCTGCG+GGG - chr6_4:34552230-34552249 None:intergenic 50.0%
CACACCCTTTGATCATCTGC+GGG - chr6_4:34552231-34552250 None:intergenic 50.0%
GCATTGAGTAAGCTTCCCAC+TGG + chr6_4:34552270-34552289 Msa0986360:CDS 50.0%
AGGTCCCCGCAGATGATCAA+AGG + chr6_4:34552223-34552242 Msa0986360:CDS 55.0%
GGTCCCCGCAGATGATCAAA+GGG + chr6_4:34552224-34552243 Msa0986360:CDS 55.0%
AGGTCCCCGCAGATGATCAA+AGG + chr6_4:34552223-34552242 Msa0986360:CDS 55.0%
GGTCCCCGCAGATGATCAAA+GGG + chr6_4:34552224-34552243 Msa0986360:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 34552210 34553081 34552210 ID=Msa0986360;Name=Msa0986360
chr6_4 mRNA 34552210 34553081 34552210 ID=Msa0986360-mRNA-1;Parent=Msa0986360;Name=Msa0986360-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|0|0.5|2|0|95
chr6_4 exon 34552210 34552371 34552210 ID=Msa0986360-mRNA-1:exon:8917;Parent=Msa0986360-mRNA-1
chr6_4 exon 34552956 34553081 34552956 ID=Msa0986360-mRNA-1:exon:8918;Parent=Msa0986360-mRNA-1
chr6_4 CDS 34552210 34552371 34552210 ID=Msa0986360-mRNA-1:cds;Parent=Msa0986360-mRNA-1
chr6_4 CDS 34552956 34553081 34552956 ID=Msa0986360-mRNA-1:cds;Parent=Msa0986360-mRNA-1
Gene Sequence

>Msa0986360

ATGACACAGGAACAGGTCCCCGCAGATGATCAAAGGGTGTGTGCTTTCTTAATTGAGAAGGCATTGAGTAAGCTTCCCACTGGGGAAGAGCAAATAGTTGCAATATTTGATCTTAGAGGTTTTGCTCCAGAAAATGCTGATCTTAAATACTTGAATTTCTTGAAACTCTCAAGAAGGAATATTTTACAGATGAAACTTTGCCACCAAATTGGTGGCAAAGTTTCATCTGTAAAATATTCCTTCTTGAGAGTTTCTGCAGAGCAAAATTTAACCTGTAAAGGAAAATGA

Protein sequence

>Msa0986360

MTQEQVPADDQRVCAFLIEKALSKLPTGEEQIVAIFDLRGFAPENADLKYLNFLKLSRRNILQMKLCHQIGGKVSSVKYSFLRVSAEQNLTCKGK*