AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0989060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCGTGGATTTGTGTAGTA+TGG 0.256278 6_4:+44288806 Msa0989060:CDS
GAGCTACCTGAGAGAGTTAC+AGG 0.324996 6_4:+44289842 Msa0989060:CDS
TATAAATCAAAGTACCTATA+TGG 0.351425 6_4:-44289894 None:intergenic
GTAGTATGGATTGGCTTGTT+TGG 0.381072 6_4:+44288820 Msa0989060:CDS
GTGTTAAAGACCAAGGGTTA+TGG 0.386926 6_4:+44288871 Msa0989060:CDS
CTATAATTACTTCACCATAT+AGG 0.420064 6_4:+44289880 Msa0989060:CDS
ATTGGCTTGTTTGGAGATGT+TGG 0.461764 6_4:+44288829 Msa0989060:CDS
TCGTAACCTGTAACTCTCTC+AGG 0.465100 6_4:-44289848 None:intergenic
TGGAGATGTTGGCTTTGTCT+AGG 0.492035 6_4:+44288840 Msa0989060:CDS
TGGATTTGTGTAGTATGGAT+TGG 0.527197 6_4:+44288811 Msa0989060:CDS
ACACAGGGCAGATAAGTTAG+TGG 0.574770 6_4:+44289818 Msa0989060:intron
GACTCTTGTGTTAAAGACCA+AGG 0.579268 6_4:+44288864 Msa0989060:CDS
TATAAACATACCATAACCCT+TGG 0.580976 6_4:-44288881 None:intergenic
ACTCTTGTGTTAAAGACCAA+GGG 0.605087 6_4:+44288865 Msa0989060:CDS
CTAACATATGCGAATCCTCG+TGG 0.630806 6_4:+44288791 None:intergenic
GAGAGAGTTACAGGTTACGA+AGG 0.700662 6_4:+44289851 Msa0989060:CDS
CCATACTACACAAATCCACG+AGG 0.750810 6_4:-44288806 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTAATGTAAAAAATATA+GGG - chr6_4:44289319-44289338 None:intergenic 10.0%
!! ATTGTTAATGTAAAAAATAT+AGG - chr6_4:44289320-44289339 None:intergenic 10.0%
!! TTGTTAATGTAAAAAATATA+GGG - chr6_4:44289319-44289338 None:intergenic 10.0%
!! ATTGTTAATGTAAAAAATAT+AGG - chr6_4:44289320-44289339 None:intergenic 10.0%
!! TAGTTTAATTAAGAAAAGTA+AGG + chr6_4:44289070-44289089 Msa0989060:intron 15.0%
!! TAGTTTAATTAAGAAAAGTA+AGG + chr6_4:44289070-44289089 Msa0989060:intron 15.0%
!!! CTTTTCTTAATTAAACTAAC+AGG - chr6_4:44289068-44289087 None:intergenic 20.0%
!! ATGTCTTAAAATAACTTTGT+AGG - chr6_4:44289423-44289442 None:intergenic 20.0%
!!! TATAAATCAAAGTACCTATA+TGG - chr6_4:44289897-44289916 None:intergenic 20.0%
!!! TACTTTGATTTATACAAGTT+GGG + chr6_4:44289903-44289922 Msa0989060:intron 20.0%
!!! CTTTTCTTAATTAAACTAAC+AGG - chr6_4:44289068-44289087 None:intergenic 20.0%
!! ATGTCTTAAAATAACTTTGT+AGG - chr6_4:44289423-44289442 None:intergenic 20.0%
!!! TATAAATCAAAGTACCTATA+TGG - chr6_4:44289897-44289916 None:intergenic 20.0%
!!! TACTTTGATTTATACAAGTT+GGG + chr6_4:44289903-44289922 Msa0989060:intron 20.0%
! GTAAAATATTGTGTAGAGTA+TGG + chr6_4:44288918-44288937 Msa0989060:intron 25.0%
! CTTGTTAGTATGTTATAGTA+TGG + chr6_4:44288972-44288991 Msa0989060:intron 25.0%
! CTATAATTACTTCACCATAT+AGG + chr6_4:44289880-44289899 Msa0989060:CDS 25.0%
!! GTACTTTGATTTATACAAGT+TGG + chr6_4:44289902-44289921 Msa0989060:intron 25.0%
!! ACTTTGATTTATACAAGTTG+GGG + chr6_4:44289904-44289923 Msa0989060:intron 25.0%
! GTAAAATATTGTGTAGAGTA+TGG + chr6_4:44288918-44288937 Msa0989060:intron 25.0%
! CTTGTTAGTATGTTATAGTA+TGG + chr6_4:44288972-44288991 Msa0989060:intron 25.0%
! CTATAATTACTTCACCATAT+AGG + chr6_4:44289880-44289899 Msa0989060:CDS 25.0%
!! GTACTTTGATTTATACAAGT+TGG + chr6_4:44289902-44289921 Msa0989060:intron 25.0%
!! ACTTTGATTTATACAAGTTG+GGG + chr6_4:44289904-44289923 Msa0989060:intron 25.0%
TATAAACATACCATAACCCT+TGG - chr6_4:44288884-44288903 None:intergenic 30.0%
AAGGGTTATGGTATGTTTAT+AGG + chr6_4:44288883-44288902 Msa0989060:intron 30.0%
ATATTGTGTAGAGTATGGTA+TGG + chr6_4:44288923-44288942 Msa0989060:intron 30.0%
!! CAGCTACTTGTATTTTAGAA+AGG - chr6_4:44289151-44289170 None:intergenic 30.0%
AGCTATAAACACAACTATGT+TGG + chr6_4:44289394-44289413 Msa0989060:intron 30.0%
TATACTGAAGAAAGACAATG+AGG - chr6_4:44289504-44289523 None:intergenic 30.0%
!! TTTTAGGAAGTTATGTGTAC+TGG - chr6_4:44289648-44289667 None:intergenic 30.0%
AGCTTACATCACAAAAAAGA+AGG - chr6_4:44289766-44289785 None:intergenic 30.0%
ATAGGTACACAAAGAAAAAG+AGG - chr6_4:44289963-44289982 None:intergenic 30.0%
! ACAAATGTGAGAAGTTTTCT+TGG + chr6_4:44289992-44290011 Msa0989060:CDS 30.0%
TATAAACATACCATAACCCT+TGG - chr6_4:44288884-44288903 None:intergenic 30.0%
AAGGGTTATGGTATGTTTAT+AGG + chr6_4:44288883-44288902 Msa0989060:intron 30.0%
ATATTGTGTAGAGTATGGTA+TGG + chr6_4:44288923-44288942 Msa0989060:intron 30.0%
!! CAGCTACTTGTATTTTAGAA+AGG - chr6_4:44289151-44289170 None:intergenic 30.0%
AGCTATAAACACAACTATGT+TGG + chr6_4:44289394-44289413 Msa0989060:intron 30.0%
TATACTGAAGAAAGACAATG+AGG - chr6_4:44289504-44289523 None:intergenic 30.0%
!! TTTTAGGAAGTTATGTGTAC+TGG - chr6_4:44289648-44289667 None:intergenic 30.0%
AGCTTACATCACAAAAAAGA+AGG - chr6_4:44289766-44289785 None:intergenic 30.0%
ATAGGTACACAAAGAAAAAG+AGG - chr6_4:44289963-44289982 None:intergenic 30.0%
! ACAAATGTGAGAAGTTTTCT+TGG + chr6_4:44289992-44290011 Msa0989060:CDS 30.0%
TGGATTTGTGTAGTATGGAT+TGG + chr6_4:44288811-44288830 Msa0989060:CDS 35.0%
ACTCTTGTGTTAAAGACCAA+GGG + chr6_4:44288865-44288884 Msa0989060:CDS 35.0%
!! TGTTTATAGGAGCACTAACA+AGG + chr6_4:44288896-44288915 Msa0989060:intron 35.0%
TAGCTGAAGTATGATTCACA+AGG + chr6_4:44289165-44289184 Msa0989060:intron 35.0%
AGCTGAAGTATGATTCACAA+GGG + chr6_4:44289166-44289185 Msa0989060:intron 35.0%
TCAGATATGCTAGTAAGCTA+TGG + chr6_4:44289240-44289259 Msa0989060:intron 35.0%
ATCTAACACGACATTAACAC+GGG - chr6_4:44289352-44289371 None:intergenic 35.0%
CATCTAACACGACATTAACA+CGG - chr6_4:44289353-44289372 None:intergenic 35.0%
TGAACTGAAACTGAAACTTC+AGG - chr6_4:44289451-44289470 None:intergenic 35.0%
! ACTATGCAACACATTGACTA+TGG + chr6_4:44289569-44289588 Msa0989060:intron 35.0%
! CAAACTGAACACTGCATTTT+AGG - chr6_4:44289664-44289683 None:intergenic 35.0%
CTAAAATGCAGTGTTCAGTT+TGG + chr6_4:44289662-44289681 Msa0989060:intron 35.0%
! TTTTTGTGTCTGTTGTACAC+AGG + chr6_4:44289802-44289821 Msa0989060:intron 35.0%
! TTTTGTGTCTGTTGTACACA+GGG + chr6_4:44289803-44289822 Msa0989060:intron 35.0%
CACATTTGTCTCTACATGAT+AGG - chr6_4:44289981-44290000 None:intergenic 35.0%
! TGAGAAGTTTTCTTGGCTTA+AGG + chr6_4:44289999-44290018 Msa0989060:CDS 35.0%
TGGATTTGTGTAGTATGGAT+TGG + chr6_4:44288811-44288830 Msa0989060:CDS 35.0%
ACTCTTGTGTTAAAGACCAA+GGG + chr6_4:44288865-44288884 Msa0989060:CDS 35.0%
!! TGTTTATAGGAGCACTAACA+AGG + chr6_4:44288896-44288915 Msa0989060:intron 35.0%
TAGCTGAAGTATGATTCACA+AGG + chr6_4:44289165-44289184 Msa0989060:intron 35.0%
AGCTGAAGTATGATTCACAA+GGG + chr6_4:44289166-44289185 Msa0989060:intron 35.0%
TCAGATATGCTAGTAAGCTA+TGG + chr6_4:44289240-44289259 Msa0989060:intron 35.0%
ATCTAACACGACATTAACAC+GGG - chr6_4:44289352-44289371 None:intergenic 35.0%
CATCTAACACGACATTAACA+CGG - chr6_4:44289353-44289372 None:intergenic 35.0%
TGAACTGAAACTGAAACTTC+AGG - chr6_4:44289451-44289470 None:intergenic 35.0%
! ACTATGCAACACATTGACTA+TGG + chr6_4:44289569-44289588 Msa0989060:intron 35.0%
! CAAACTGAACACTGCATTTT+AGG - chr6_4:44289664-44289683 None:intergenic 35.0%
CTAAAATGCAGTGTTCAGTT+TGG + chr6_4:44289662-44289681 Msa0989060:intron 35.0%
! TTTTTGTGTCTGTTGTACAC+AGG + chr6_4:44289802-44289821 Msa0989060:intron 35.0%
! TTTTGTGTCTGTTGTACACA+GGG + chr6_4:44289803-44289822 Msa0989060:intron 35.0%
CACATTTGTCTCTACATGAT+AGG - chr6_4:44289981-44290000 None:intergenic 35.0%
! TGAGAAGTTTTCTTGGCTTA+AGG + chr6_4:44289999-44290018 Msa0989060:CDS 35.0%
!! GTAGTATGGATTGGCTTGTT+TGG + chr6_4:44288820-44288839 Msa0989060:CDS 40.0%
!! ATTGGCTTGTTTGGAGATGT+TGG + chr6_4:44288829-44288848 Msa0989060:CDS 40.0%
GACTCTTGTGTTAAAGACCA+AGG + chr6_4:44288864-44288883 Msa0989060:CDS 40.0%
GTGTTAAAGACCAAGGGTTA+TGG + chr6_4:44288871-44288890 Msa0989060:CDS 40.0%
GTTAGATGTCCGTGCTTTAT+AGG + chr6_4:44289365-44289384 Msa0989060:intron 40.0%
AGTCATGAACTGATCCATTG+TGG + chr6_4:44289471-44289490 Msa0989060:intron 40.0%
GTCATGAACTGATCCATTGT+GGG + chr6_4:44289472-44289491 Msa0989060:intron 40.0%
AATGAGGAGTTATCCCACAA+TGG - chr6_4:44289488-44289507 None:intergenic 40.0%
!! GTAGTATGGATTGGCTTGTT+TGG + chr6_4:44288820-44288839 Msa0989060:CDS 40.0%
!! ATTGGCTTGTTTGGAGATGT+TGG + chr6_4:44288829-44288848 Msa0989060:CDS 40.0%
GACTCTTGTGTTAAAGACCA+AGG + chr6_4:44288864-44288883 Msa0989060:CDS 40.0%
GTGTTAAAGACCAAGGGTTA+TGG + chr6_4:44288871-44288890 Msa0989060:CDS 40.0%
GTTAGATGTCCGTGCTTTAT+AGG + chr6_4:44289365-44289384 Msa0989060:intron 40.0%
AGTCATGAACTGATCCATTG+TGG + chr6_4:44289471-44289490 Msa0989060:intron 40.0%
GTCATGAACTGATCCATTGT+GGG + chr6_4:44289472-44289491 Msa0989060:intron 40.0%
AATGAGGAGTTATCCCACAA+TGG - chr6_4:44289488-44289507 None:intergenic 40.0%
CCATACTACACAAATCCACG+AGG - chr6_4:44288809-44288828 None:intergenic 45.0%
CCTCGTGGATTTGTGTAGTA+TGG + chr6_4:44288806-44288825 Msa0989060:CDS 45.0%
!! TGGAGATGTTGGCTTTGTCT+AGG + chr6_4:44288840-44288859 Msa0989060:CDS 45.0%
AGATGTCCGTGCTTTATAGG+TGG + chr6_4:44289368-44289387 Msa0989060:intron 45.0%
GATGTCCGTGCTTTATAGGT+GGG + chr6_4:44289369-44289388 Msa0989060:intron 45.0%
! TGCAGTGTTCAGTTTGGAAC+AGG + chr6_4:44289668-44289687 Msa0989060:intron 45.0%
ACACAGGGCAGATAAGTTAG+TGG + chr6_4:44289818-44289837 Msa0989060:intron 45.0%
TCGTAACCTGTAACTCTCTC+AGG - chr6_4:44289851-44289870 None:intergenic 45.0%
GAGAGAGTTACAGGTTACGA+AGG + chr6_4:44289851-44289870 Msa0989060:CDS 45.0%
CCATACTACACAAATCCACG+AGG - chr6_4:44288809-44288828 None:intergenic 45.0%
CCTCGTGGATTTGTGTAGTA+TGG + chr6_4:44288806-44288825 Msa0989060:CDS 45.0%
!! TGGAGATGTTGGCTTTGTCT+AGG + chr6_4:44288840-44288859 Msa0989060:CDS 45.0%
AGATGTCCGTGCTTTATAGG+TGG + chr6_4:44289368-44289387 Msa0989060:intron 45.0%
GATGTCCGTGCTTTATAGGT+GGG + chr6_4:44289369-44289388 Msa0989060:intron 45.0%
! TGCAGTGTTCAGTTTGGAAC+AGG + chr6_4:44289668-44289687 Msa0989060:intron 45.0%
ACACAGGGCAGATAAGTTAG+TGG + chr6_4:44289818-44289837 Msa0989060:intron 45.0%
TCGTAACCTGTAACTCTCTC+AGG - chr6_4:44289851-44289870 None:intergenic 45.0%
GAGAGAGTTACAGGTTACGA+AGG + chr6_4:44289851-44289870 Msa0989060:CDS 45.0%
GCTAGCCCACCTATAAAGCA+CGG - chr6_4:44289377-44289396 None:intergenic 50.0%
GAGCTACCTGAGAGAGTTAC+AGG + chr6_4:44289842-44289861 Msa0989060:CDS 50.0%
GCTAGCCCACCTATAAAGCA+CGG - chr6_4:44289377-44289396 None:intergenic 50.0%
GAGCTACCTGAGAGAGTTAC+AGG + chr6_4:44289842-44289861 Msa0989060:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 44288798 44290026 44288798 ID=Msa0989060;Name=Msa0989060
chr6_4 mRNA 44288798 44290026 44288798 ID=Msa0989060-mRNA-1;Parent=Msa0989060;Name=Msa0989060-mRNA-1;_AED=0.51;_eAED=0.70;_QI=0|0|0|1|0.5|0.66|3|0|69
chr6_4 exon 44288798 44288892 44288798 ID=Msa0989060-mRNA-1:exon:10176;Parent=Msa0989060-mRNA-1
chr6_4 exon 44289824 44289901 44289824 ID=Msa0989060-mRNA-1:exon:10177;Parent=Msa0989060-mRNA-1
chr6_4 exon 44289990 44290026 44289990 ID=Msa0989060-mRNA-1:exon:10178;Parent=Msa0989060-mRNA-1
chr6_4 CDS 44288798 44288892 44288798 ID=Msa0989060-mRNA-1:cds;Parent=Msa0989060-mRNA-1
chr6_4 CDS 44289824 44289901 44289824 ID=Msa0989060-mRNA-1:cds;Parent=Msa0989060-mRNA-1
chr6_4 CDS 44289990 44290026 44289990 ID=Msa0989060-mRNA-1:cds;Parent=Msa0989060-mRNA-1
Gene Sequence

>Msa0989060

ATGCGAATCCTCGTGGATTTGTGTAGTATGGATTGGCTTGTTTGGAGATGTTGGCTTTGTCTAGGTGACTCTTGTGTTAAAGACCAAGGGTTATGGGCAGATAAGTTAGTGGAGAGCTACCTGAGAGAGTTACAGGTTACGAAGGCGTGTTCTATAATTACTTCACCATATAGAGACAAATGTGAGAAGTTTTCTTGGCTTAAGGTGTAA

Protein sequence

>Msa0989060

MRILVDLCSMDWLVWRCWLCLGDSCVKDQGLWADKLVESYLRELQVTKACSIITSPYRDKCEKFSWLKV*