AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTGTTGTCGTAATAATT+TGG 0.248203 8_1:+71247964 None:intergenic
AGTTCTTACCTTACAAGCTT+TGG 0.288666 8_1:+71249064 None:intergenic
GTTCTTACCTTACAAGCTTT+GGG 0.309532 8_1:+71249065 None:intergenic
CAAAATAAACGGCTCAGATT+TGG 0.322391 8_1:+71247705 None:intergenic
CCTTACAAGCTTTGGGCCTT+CGG 0.363556 8_1:+71249072 None:intergenic
AGAGGTAGGGGTGGCAAAAC+GGG 0.387788 8_1:-71248447 Msa1211520:CDS
CCGAAGGCCCAAAGCTTGTA+AGG 0.428068 8_1:-71249072 Msa1211520:intron
TAGAGGTAGGGGTGGCAAAA+CGG 0.446144 8_1:-71248448 Msa1211520:CDS
TACTATTAATAATGAGAGTC+AGG 0.449739 8_1:-71247764 Msa1211520:three_prime_UTR
GATGATGATGAGAGGGATGC+TGG 0.473439 8_1:-71247997 Msa1211520:CDS
CTTAGTAGCATCTAGAGGTA+GGG 0.489005 8_1:-71248460 Msa1211520:CDS
GCTTAGTAGCATCTAGAGGT+AGG 0.497030 8_1:-71248461 Msa1211520:CDS
TGTGCTGAACTTGTCCCCTC+CGG 0.501326 8_1:-71248378 Msa1211520:intron
AAAAGACATGTATATTCTCT+AGG 0.506853 8_1:-71249952 Msa1211520:CDS
AGGAAGGAGAGTAGATCAGA+TGG 0.509310 8_1:-71249932 Msa1211520:CDS
ATATGGCAAGAACTCGGCTT+AGG 0.513084 8_1:-71249993 Msa1211520:CDS
GACATGTATATTCTCTAGGA+AGG 0.534062 8_1:-71249948 Msa1211520:CDS
TTAGTAGCATCTAGAGGTAG+GGG 0.549294 8_1:-71248459 Msa1211520:CDS
ATGGAGAGTAGTCAAATCAC+TGG 0.553657 8_1:-71249913 Msa1211520:CDS
ATCAAGAATCTCATCATAGC+CGG 0.557413 8_1:+71248491 None:intergenic
TCAACGAATCCGTTGACACC+TGG 0.563423 8_1:+71247730 None:intergenic
CCGTTGACACCTGGTGTCAA+CGG 0.565625 8_1:+71247739 None:intergenic
AGTCAGGATCCGTTGACACC+AGG 0.572372 8_1:-71247748 Msa1211520:three_prime_UTR
CCGTTGACACCAGGTGTCAA+CGG 0.584456 8_1:-71247739 Msa1211520:three_prime_UTR
GGGCAATGATGATGATGAGA+GGG 0.586025 8_1:-71248004 Msa1211520:CDS
TGGCAAGAACTCGGCTTAGG+CGG 0.596853 8_1:-71249990 Msa1211520:CDS
TAGCAAATATGGCAAGAACT+CGG 0.608510 8_1:-71249999 None:intergenic
TAATTGTTATGTAGATGTAG+TGG 0.613691 8_1:-71248025 Msa1211520:intron
CATAGCTTAGTAGCATCTAG+AGG 0.618012 8_1:-71248465 Msa1211520:CDS
GCTGTAATTGCTAATAACAC+CGG 0.643730 8_1:-71248510 Msa1211520:CDS
AATTGTTATGTAGATGTAGT+GGG 0.644881 8_1:-71248024 Msa1211520:intron
TGGGCAATGATGATGATGAG+AGG 0.661625 8_1:-71248005 Msa1211520:CDS
GTAGCATCTAGAGGTAGGGG+TGG 0.665546 8_1:-71248456 Msa1211520:CDS
AGGAAGCAGAAACTATCCGA+AGG 0.676012 8_1:-71249088 Msa1211520:CDS
ACGAGACATGCCAACCGGAG+GGG 0.697648 8_1:+71248364 None:intergenic
GAGAGTAGTCAAATCACTGG+AGG 0.711176 8_1:-71249910 Msa1211520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAAAATTTAAAATAAAAC+AGG + chr8_1:71248517-71248536 None:intergenic 10.0%
!!! TTTATTTATTTGTTTTATGT+TGG - chr8_1:71249862-71249881 Msa1211520:CDS 10.0%
!!! ATGAAAATTTAAAATAAAAC+AGG + chr8_1:71248517-71248536 None:intergenic 10.0%
!!! TTTATTTATTTGTTTTATGT+TGG - chr8_1:71249862-71249881 Msa1211520:CDS 10.0%
!!! TTAAATTTCATCTTTGATTT+TGG - chr8_1:71248539-71248558 Msa1211520:intron 15.0%
!!! TAAATTTCATCTTTGATTTT+GGG - chr8_1:71248540-71248559 Msa1211520:intron 15.0%
!! AAAAGAAAAACAAAAGAAAA+AGG - chr8_1:71248600-71248619 Msa1211520:intron 15.0%
!! TTAGCTTCTTAAAAAAAAAA+TGG + chr8_1:71248777-71248796 None:intergenic 15.0%
!! CATGTTAATAAATCTTATAA+AGG + chr8_1:71248983-71249002 None:intergenic 15.0%
!! AAAATGCTAATACAAATAAA+AGG + chr8_1:71249597-71249616 None:intergenic 15.0%
!!! TTAAATTTCATCTTTGATTT+TGG - chr8_1:71248539-71248558 Msa1211520:intron 15.0%
!!! TAAATTTCATCTTTGATTTT+GGG - chr8_1:71248540-71248559 Msa1211520:intron 15.0%
!! AAAAGAAAAACAAAAGAAAA+AGG - chr8_1:71248600-71248619 Msa1211520:intron 15.0%
!! TTAGCTTCTTAAAAAAAAAA+TGG + chr8_1:71248777-71248796 None:intergenic 15.0%
!! CATGTTAATAAATCTTATAA+AGG + chr8_1:71248983-71249002 None:intergenic 15.0%
!! AAAATGCTAATACAAATAAA+AGG + chr8_1:71249597-71249616 None:intergenic 15.0%
!!! CTCTTATGAATATTTTGATT+TGG - chr8_1:71247926-71247945 Msa1211520:exon 20.0%
!!! GTATTTTCGAAAATATATGT+TGG - chr8_1:71248748-71248767 Msa1211520:intron 20.0%
!! AGAAGCTTATATAAGTTATT+TGG - chr8_1:71249504-71249523 Msa1211520:intron 20.0%
!! GAAGCTTATATAAGTTATTT+GGG - chr8_1:71249505-71249524 Msa1211520:intron 20.0%
!! AATAAAAGGTCAACAAAAAA+AGG + chr8_1:71249583-71249602 None:intergenic 20.0%
!!! ATTTATTTGTTTTATGTTGG+TGG - chr8_1:71249865-71249884 Msa1211520:CDS 20.0%
!!! CTCTTATGAATATTTTGATT+TGG - chr8_1:71247926-71247945 Msa1211520:exon 20.0%
!!! GTATTTTCGAAAATATATGT+TGG - chr8_1:71248748-71248767 Msa1211520:intron 20.0%
!! AGAAGCTTATATAAGTTATT+TGG - chr8_1:71249504-71249523 Msa1211520:intron 20.0%
!! GAAGCTTATATAAGTTATTT+GGG - chr8_1:71249505-71249524 Msa1211520:intron 20.0%
!! AATAAAAGGTCAACAAAAAA+AGG + chr8_1:71249583-71249602 None:intergenic 20.0%
!!! ATTTATTTGTTTTATGTTGG+TGG - chr8_1:71249865-71249884 Msa1211520:CDS 20.0%
! AAAAGACATGTATATTCTCT+AGG - chr8_1:71247756-71247775 Msa1211520:three_prime_UTR 25.0%
!! TGAGATTAAAATAAGCATTG+AGG - chr8_1:71248032-71248051 Msa1211520:intron 25.0%
! AAAAGACATGTATATTCTCT+AGG - chr8_1:71247756-71247775 Msa1211520:three_prime_UTR 25.0%
!! GTTTCTAATATAGTTTTTCG+AGG + chr8_1:71248319-71248338 None:intergenic 25.0%
! TGATATATGATAGAACATCA+TGG - chr8_1:71248361-71248380 Msa1211520:intron 25.0%
!!! AGTTTCTTTTTGTTTTGTGT+AGG - chr8_1:71248564-71248583 Msa1211520:intron 25.0%
! ACATTTACAAAACAAGCTTT+GGG + chr8_1:71248680-71248699 None:intergenic 25.0%
! AACATTTACAAAACAAGCTT+TGG + chr8_1:71248681-71248700 None:intergenic 25.0%
! TTATTGTGCTATGTATACAT+TGG - chr8_1:71248699-71248718 Msa1211520:intron 25.0%
! GTATAAATTGCGATTAGAAA+TGG + chr8_1:71248915-71248934 None:intergenic 25.0%
!! TTTAGTTCGTTGAAAAAACA+GGG - chr8_1:71249366-71249385 Msa1211520:intron 25.0%
!!! TTTCCTTCAAATTTTGATGT+GGG - chr8_1:71249423-71249442 Msa1211520:intron 25.0%
! AAGTTCATGTTAACAAAAAG+AGG + chr8_1:71249472-71249491 None:intergenic 25.0%
! TAATTGTTATGTAGATGTAG+TGG - chr8_1:71249683-71249702 Msa1211520:intron 25.0%
! AATTGTTATGTAGATGTAGT+GGG - chr8_1:71249684-71249703 Msa1211520:intron 25.0%
! ATGTTGTTGTCGTAATAATT+TGG + chr8_1:71249747-71249766 None:intergenic 25.0%
!!! GAGACTTATTTTATGCATTT+TGG - chr8_1:71249831-71249850 Msa1211520:intron 25.0%
!!! ATTTTATGCATTTTGGTTGT+TGG - chr8_1:71249838-71249857 Msa1211520:CDS 25.0%
! TACTATTAATAATGAGAGTC+AGG - chr8_1:71249944-71249963 Msa1211520:CDS 25.0%
! AAAAGACATGTATATTCTCT+AGG - chr8_1:71247756-71247775 Msa1211520:three_prime_UTR 25.0%
!! TGAGATTAAAATAAGCATTG+AGG - chr8_1:71248032-71248051 Msa1211520:intron 25.0%
! AAAAGACATGTATATTCTCT+AGG - chr8_1:71247756-71247775 Msa1211520:three_prime_UTR 25.0%
!! GTTTCTAATATAGTTTTTCG+AGG + chr8_1:71248319-71248338 None:intergenic 25.0%
! TGATATATGATAGAACATCA+TGG - chr8_1:71248361-71248380 Msa1211520:intron 25.0%
!!! AGTTTCTTTTTGTTTTGTGT+AGG - chr8_1:71248564-71248583 Msa1211520:intron 25.0%
! ACATTTACAAAACAAGCTTT+GGG + chr8_1:71248680-71248699 None:intergenic 25.0%
! AACATTTACAAAACAAGCTT+TGG + chr8_1:71248681-71248700 None:intergenic 25.0%
! TTATTGTGCTATGTATACAT+TGG - chr8_1:71248699-71248718 Msa1211520:intron 25.0%
! GTATAAATTGCGATTAGAAA+TGG + chr8_1:71248915-71248934 None:intergenic 25.0%
!! TTTAGTTCGTTGAAAAAACA+GGG - chr8_1:71249366-71249385 Msa1211520:intron 25.0%
!!! TTTCCTTCAAATTTTGATGT+GGG - chr8_1:71249423-71249442 Msa1211520:intron 25.0%
! AAGTTCATGTTAACAAAAAG+AGG + chr8_1:71249472-71249491 None:intergenic 25.0%
! TAATTGTTATGTAGATGTAG+TGG - chr8_1:71249683-71249702 Msa1211520:intron 25.0%
! AATTGTTATGTAGATGTAGT+GGG - chr8_1:71249684-71249703 Msa1211520:intron 25.0%
! ATGTTGTTGTCGTAATAATT+TGG + chr8_1:71249747-71249766 None:intergenic 25.0%
!!! GAGACTTATTTTATGCATTT+TGG - chr8_1:71249831-71249850 Msa1211520:intron 25.0%
!!! ATTTTATGCATTTTGGTTGT+TGG - chr8_1:71249838-71249857 Msa1211520:CDS 25.0%
! TACTATTAATAATGAGAGTC+AGG - chr8_1:71249944-71249963 Msa1211520:CDS 25.0%
AGCAACAAAAATAAGTTACG+TGG + chr8_1:71247849-71247868 None:intergenic 30.0%
TTACTCAATCAATTGCAAGT+TGG - chr8_1:71247963-71247982 Msa1211520:CDS 30.0%
! CGAGATTAAAATAAGCATTG+AGG - chr8_1:71248122-71248141 Msa1211520:intron 30.0%
AGAATGTTATGTTGGATATG+CGG - chr8_1:71248150-71248169 Msa1211520:intron 30.0%
CAAGATAGTACTAGCAAATA+TGG - chr8_1:71248183-71248202 Msa1211520:intron 30.0%
!! ATAAGTTGGTTGTTGTTGTT+TGG - chr8_1:71248419-71248438 Msa1211520:CDS 30.0%
ATCTTATAAAGGTCTGCATT+GGG + chr8_1:71248972-71248991 None:intergenic 30.0%
AATCTTATAAAGGTCTGCAT+TGG + chr8_1:71248973-71248992 None:intergenic 30.0%
TCATCTGTGAATCATGTATT+GGG - chr8_1:71249100-71249119 Msa1211520:CDS 30.0%
! GTTTAGTTCGTTGAAAAAAC+AGG - chr8_1:71249365-71249384 Msa1211520:intron 30.0%
!! CTTTCCTTCAAATTTTGATG+TGG - chr8_1:71249422-71249441 Msa1211520:intron 30.0%
AATCCCACATCAAAATTTGA+AGG + chr8_1:71249429-71249448 None:intergenic 30.0%
! AAGTTATTTGGGTTAAGTGA+AGG - chr8_1:71249516-71249535 Msa1211520:intron 30.0%
TACTACAAAAATTGTGCTGT+TGG - chr8_1:71249801-71249820 Msa1211520:intron 30.0%
AGCAACAAAAATAAGTTACG+TGG + chr8_1:71247849-71247868 None:intergenic 30.0%
TTACTCAATCAATTGCAAGT+TGG - chr8_1:71247963-71247982 Msa1211520:CDS 30.0%
! CGAGATTAAAATAAGCATTG+AGG - chr8_1:71248122-71248141 Msa1211520:intron 30.0%
AGAATGTTATGTTGGATATG+CGG - chr8_1:71248150-71248169 Msa1211520:intron 30.0%
CAAGATAGTACTAGCAAATA+TGG - chr8_1:71248183-71248202 Msa1211520:intron 30.0%
!! ATAAGTTGGTTGTTGTTGTT+TGG - chr8_1:71248419-71248438 Msa1211520:CDS 30.0%
ATCTTATAAAGGTCTGCATT+GGG + chr8_1:71248972-71248991 None:intergenic 30.0%
AATCTTATAAAGGTCTGCAT+TGG + chr8_1:71248973-71248992 None:intergenic 30.0%
TCATCTGTGAATCATGTATT+GGG - chr8_1:71249100-71249119 Msa1211520:CDS 30.0%
! GTTTAGTTCGTTGAAAAAAC+AGG - chr8_1:71249365-71249384 Msa1211520:intron 30.0%
!! CTTTCCTTCAAATTTTGATG+TGG - chr8_1:71249422-71249441 Msa1211520:intron 30.0%
AATCCCACATCAAAATTTGA+AGG + chr8_1:71249429-71249448 None:intergenic 30.0%
! AAGTTATTTGGGTTAAGTGA+AGG - chr8_1:71249516-71249535 Msa1211520:intron 30.0%
TACTACAAAAATTGTGCTGT+TGG - chr8_1:71249801-71249820 Msa1211520:intron 30.0%
! GACATGTATATTCTCTAGGA+AGG - chr8_1:71247760-71247779 Msa1211520:three_prime_UTR 35.0%
AGGAGATGAGAATGTTATGT+TGG - chr8_1:71248142-71248161 Msa1211520:intron 35.0%
TAGCAAATATGGCAAGAACT+CGG - chr8_1:71248194-71248213 Msa1211520:intron 35.0%
! GACATGTATATTCTCTAGGA+AGG - chr8_1:71247760-71247779 Msa1211520:three_prime_UTR 35.0%
CAACCAACTTATCACTAAGT+GGG + chr8_1:71248411-71248430 None:intergenic 35.0%
ACAACCAACTTATCACTAAG+TGG + chr8_1:71248412-71248431 None:intergenic 35.0%
GTTCTTACCTTACAAGCTTT+GGG + chr8_1:71248646-71248665 None:intergenic 35.0%
AGTTCTTACCTTACAAGCTT+TGG + chr8_1:71248647-71248666 None:intergenic 35.0%
TCTTATAAAGGTCTGCATTG+GGG + chr8_1:71248971-71248990 None:intergenic 35.0%
ATGCAATACTAATAACCACG+TGG + chr8_1:71249057-71249076 None:intergenic 35.0%
! TTCACAGATGACTTCTAGAA+CGG + chr8_1:71249091-71249110 None:intergenic 35.0%
GTCATCTGTGAATCATGTAT+TGG - chr8_1:71249099-71249118 Msa1211520:CDS 35.0%
AACTGTATATTGTGCTACAC+AGG + chr8_1:71249141-71249160 None:intergenic 35.0%
!!! TCATCTGTTTTAGTTTGAGC+AGG - chr8_1:71249176-71249195 Msa1211520:intron 35.0%
GCTGTAATTGCTAATAACAC+CGG - chr8_1:71249198-71249217 Msa1211520:intron 35.0%
ATCAAGAATCTCATCATAGC+CGG + chr8_1:71249220-71249239 None:intergenic 35.0%
! GACATGTATATTCTCTAGGA+AGG - chr8_1:71247760-71247779 Msa1211520:three_prime_UTR 35.0%
AGGAGATGAGAATGTTATGT+TGG - chr8_1:71248142-71248161 Msa1211520:intron 35.0%
TAGCAAATATGGCAAGAACT+CGG - chr8_1:71248194-71248213 Msa1211520:intron 35.0%
! GACATGTATATTCTCTAGGA+AGG - chr8_1:71247760-71247779 Msa1211520:three_prime_UTR 35.0%
CAACCAACTTATCACTAAGT+GGG + chr8_1:71248411-71248430 None:intergenic 35.0%
ACAACCAACTTATCACTAAG+TGG + chr8_1:71248412-71248431 None:intergenic 35.0%
GTTCTTACCTTACAAGCTTT+GGG + chr8_1:71248646-71248665 None:intergenic 35.0%
AGTTCTTACCTTACAAGCTT+TGG + chr8_1:71248647-71248666 None:intergenic 35.0%
TCTTATAAAGGTCTGCATTG+GGG + chr8_1:71248971-71248990 None:intergenic 35.0%
ATGCAATACTAATAACCACG+TGG + chr8_1:71249057-71249076 None:intergenic 35.0%
! TTCACAGATGACTTCTAGAA+CGG + chr8_1:71249091-71249110 None:intergenic 35.0%
GTCATCTGTGAATCATGTAT+TGG - chr8_1:71249099-71249118 Msa1211520:CDS 35.0%
AACTGTATATTGTGCTACAC+AGG + chr8_1:71249141-71249160 None:intergenic 35.0%
!!! TCATCTGTTTTAGTTTGAGC+AGG - chr8_1:71249176-71249195 Msa1211520:intron 35.0%
GCTGTAATTGCTAATAACAC+CGG - chr8_1:71249198-71249217 Msa1211520:intron 35.0%
ATCAAGAATCTCATCATAGC+CGG + chr8_1:71249220-71249239 None:intergenic 35.0%
ATGGAGAGTAGTCAAATCAC+TGG - chr8_1:71247795-71247814 Msa1211520:three_prime_UTR 40.0%
GGAGATGATTCATTGCAAGT+TGG - chr8_1:71248053-71248072 Msa1211520:intron 40.0%
ATGGAGAGTAGTCAAATCAC+TGG - chr8_1:71247795-71247814 Msa1211520:three_prime_UTR 40.0%
GATCCCACTTAGTGATAAGT+TGG - chr8_1:71248405-71248424 Msa1211520:CDS 40.0%
GACACCAACATAACCCATTT+GGG + chr8_1:71248893-71248912 None:intergenic 40.0%
!!! GATAGTTTTGTACTTCCACG+TGG - chr8_1:71249039-71249058 Msa1211520:intron 40.0%
CATAGCTTAGTAGCATCTAG+AGG - chr8_1:71249243-71249262 Msa1211520:intron 40.0%
CTTAGTAGCATCTAGAGGTA+GGG - chr8_1:71249248-71249267 Msa1211520:intron 40.0%
TTAGTAGCATCTAGAGGTAG+GGG - chr8_1:71249249-71249268 Msa1211520:intron 40.0%
AGTTCGTTGAAAAAACAGGG+CGG - chr8_1:71249369-71249388 Msa1211520:intron 40.0%
AAGACACGACACAAGTTAGT+GGG + chr8_1:71249405-71249424 None:intergenic 40.0%
AAAGACACGACACAAGTTAG+TGG + chr8_1:71249406-71249425 None:intergenic 40.0%
CAGTGAAGAACAACTACAGA+GGG + chr8_1:71249650-71249669 None:intergenic 40.0%
ATGGAGAGTAGTCAAATCAC+TGG - chr8_1:71247795-71247814 Msa1211520:three_prime_UTR 40.0%
GGAGATGATTCATTGCAAGT+TGG - chr8_1:71248053-71248072 Msa1211520:intron 40.0%
ATGGAGAGTAGTCAAATCAC+TGG - chr8_1:71247795-71247814 Msa1211520:three_prime_UTR 40.0%
GATCCCACTTAGTGATAAGT+TGG - chr8_1:71248405-71248424 Msa1211520:CDS 40.0%
GACACCAACATAACCCATTT+GGG + chr8_1:71248893-71248912 None:intergenic 40.0%
!!! GATAGTTTTGTACTTCCACG+TGG - chr8_1:71249039-71249058 Msa1211520:intron 40.0%
CATAGCTTAGTAGCATCTAG+AGG - chr8_1:71249243-71249262 Msa1211520:intron 40.0%
CTTAGTAGCATCTAGAGGTA+GGG - chr8_1:71249248-71249267 Msa1211520:intron 40.0%
TTAGTAGCATCTAGAGGTAG+GGG - chr8_1:71249249-71249268 Msa1211520:intron 40.0%
AGTTCGTTGAAAAAACAGGG+CGG - chr8_1:71249369-71249388 Msa1211520:intron 40.0%
AAGACACGACACAAGTTAGT+GGG + chr8_1:71249405-71249424 None:intergenic 40.0%
AAAGACACGACACAAGTTAG+TGG + chr8_1:71249406-71249425 None:intergenic 40.0%
CAGTGAAGAACAACTACAGA+GGG + chr8_1:71249650-71249669 None:intergenic 40.0%
AGGAAGGAGAGTAGATCAGA+TGG - chr8_1:71247776-71247795 Msa1211520:three_prime_UTR 45.0%
GAGAGTAGTCAAATCACTGG+AGG - chr8_1:71247798-71247817 Msa1211520:three_prime_UTR 45.0%
! TTACGTGGCTGAAGTTTTCG+AGG + chr8_1:71247834-71247853 None:intergenic 45.0%
ATATGGCAAGAACTCGGCTT+AGG - chr8_1:71248200-71248219 Msa1211520:intron 45.0%
AGGAAGGAGAGTAGATCAGA+TGG - chr8_1:71247776-71247795 Msa1211520:three_prime_UTR 45.0%
GAGAGTAGTCAAATCACTGG+AGG - chr8_1:71247798-71247817 Msa1211520:three_prime_UTR 45.0%
AGGAAGCAGAAACTATCCGA+AGG - chr8_1:71248620-71248639 Msa1211520:intron 45.0%
TAGCCCACCCAAATTAGCAT+CGG - chr8_1:71248799-71248818 Msa1211520:intron 45.0%
ATTCCCGCCGATGCTAATTT+GGG + chr8_1:71248809-71248828 None:intergenic 45.0%
GAATCGAACCTAAGACCTTG+AGG - chr8_1:71248825-71248844 Msa1211520:intron 45.0%
!! CCATTTGGGTTGGTTTGATG+GGG + chr8_1:71248879-71248898 None:intergenic 45.0%
!! CCCATTTGGGTTGGTTTGAT+GGG + chr8_1:71248880-71248899 None:intergenic 45.0%
CCCATCAAACCAACCCAAAT+GGG - chr8_1:71248877-71248896 Msa1211520:intron 45.0%
!! ACCCATTTGGGTTGGTTTGA+TGG + chr8_1:71248881-71248900 None:intergenic 45.0%
CCAACATAACCCATTTGGGT+TGG + chr8_1:71248889-71248908 None:intergenic 45.0%
!! CCAACCCAAATGGGTTATGT+TGG - chr8_1:71248886-71248905 Msa1211520:intron 45.0%
GGACACCAACATAACCCATT+TGG + chr8_1:71248894-71248913 None:intergenic 45.0%
GCTTAGTAGCATCTAGAGGT+AGG - chr8_1:71249247-71249266 Msa1211520:intron 45.0%
!! CCAAAAATAGCGGGCTAAAC+AGG + chr8_1:71249305-71249324 None:intergenic 45.0%
!! CCTGTTTAGCCCGCTATTTT+TGG - chr8_1:71249302-71249321 Msa1211520:intron 45.0%
CACAACTCGCCAAAAATAGC+GGG + chr8_1:71249314-71249333 None:intergenic 45.0%
GCACAACTCGCCAAAAATAG+CGG + chr8_1:71249315-71249334 None:intergenic 45.0%
ACTAAACGAGACATGCCAAC+CGG + chr8_1:71249352-71249371 None:intergenic 45.0%
GTTCGTTGAAAAAACAGGGC+GGG - chr8_1:71249370-71249389 Msa1211520:intron 45.0%
TTCGTTGAAAAAACAGGGCG+GGG - chr8_1:71249371-71249390 Msa1211520:intron 45.0%
GCAGTGAAGAACAACTACAG+AGG + chr8_1:71249651-71249670 None:intergenic 45.0%
! TGGGCAATGATGATGATGAG+AGG - chr8_1:71249703-71249722 Msa1211520:intron 45.0%
! GGGCAATGATGATGATGAGA+GGG - chr8_1:71249704-71249723 Msa1211520:intron 45.0%
AGGAAGGAGAGTAGATCAGA+TGG - chr8_1:71247776-71247795 Msa1211520:three_prime_UTR 45.0%
GAGAGTAGTCAAATCACTGG+AGG - chr8_1:71247798-71247817 Msa1211520:three_prime_UTR 45.0%
! TTACGTGGCTGAAGTTTTCG+AGG + chr8_1:71247834-71247853 None:intergenic 45.0%
ATATGGCAAGAACTCGGCTT+AGG - chr8_1:71248200-71248219 Msa1211520:intron 45.0%
AGGAAGGAGAGTAGATCAGA+TGG - chr8_1:71247776-71247795 Msa1211520:three_prime_UTR 45.0%
GAGAGTAGTCAAATCACTGG+AGG - chr8_1:71247798-71247817 Msa1211520:three_prime_UTR 45.0%
AGGAAGCAGAAACTATCCGA+AGG - chr8_1:71248620-71248639 Msa1211520:intron 45.0%
TAGCCCACCCAAATTAGCAT+CGG - chr8_1:71248799-71248818 Msa1211520:intron 45.0%
ATTCCCGCCGATGCTAATTT+GGG + chr8_1:71248809-71248828 None:intergenic 45.0%
GAATCGAACCTAAGACCTTG+AGG - chr8_1:71248825-71248844 Msa1211520:intron 45.0%
!! CCATTTGGGTTGGTTTGATG+GGG + chr8_1:71248879-71248898 None:intergenic 45.0%
!! CCCATTTGGGTTGGTTTGAT+GGG + chr8_1:71248880-71248899 None:intergenic 45.0%
CCCATCAAACCAACCCAAAT+GGG - chr8_1:71248877-71248896 Msa1211520:intron 45.0%
!! ACCCATTTGGGTTGGTTTGA+TGG + chr8_1:71248881-71248900 None:intergenic 45.0%
CCAACATAACCCATTTGGGT+TGG + chr8_1:71248889-71248908 None:intergenic 45.0%
!! CCAACCCAAATGGGTTATGT+TGG - chr8_1:71248886-71248905 Msa1211520:intron 45.0%
GGACACCAACATAACCCATT+TGG + chr8_1:71248894-71248913 None:intergenic 45.0%
GCTTAGTAGCATCTAGAGGT+AGG - chr8_1:71249247-71249266 Msa1211520:intron 45.0%
!! CCAAAAATAGCGGGCTAAAC+AGG + chr8_1:71249305-71249324 None:intergenic 45.0%
!! CCTGTTTAGCCCGCTATTTT+TGG - chr8_1:71249302-71249321 Msa1211520:intron 45.0%
CACAACTCGCCAAAAATAGC+GGG + chr8_1:71249314-71249333 None:intergenic 45.0%
GCACAACTCGCCAAAAATAG+CGG + chr8_1:71249315-71249334 None:intergenic 45.0%
ACTAAACGAGACATGCCAAC+CGG + chr8_1:71249352-71249371 None:intergenic 45.0%
GTTCGTTGAAAAAACAGGGC+GGG - chr8_1:71249370-71249389 Msa1211520:intron 45.0%
TTCGTTGAAAAAACAGGGCG+GGG - chr8_1:71249371-71249390 Msa1211520:intron 45.0%
GCAGTGAAGAACAACTACAG+AGG + chr8_1:71249651-71249670 None:intergenic 45.0%
! TGGGCAATGATGATGATGAG+AGG - chr8_1:71249703-71249722 Msa1211520:intron 45.0%
! GGGCAATGATGATGATGAGA+GGG - chr8_1:71249704-71249723 Msa1211520:intron 45.0%
!! CCTTACAAGCTTTGGGCCTT+CGG + chr8_1:71248639-71248658 None:intergenic 50.0%
GATTCCCGCCGATGCTAATT+TGG + chr8_1:71248810-71248829 None:intergenic 50.0%
TCGAACCTAAGACCTTGAGG+AGG - chr8_1:71248828-71248847 Msa1211520:intron 50.0%
!! TGGTTTGATGGGGTTGGCTT+GGG + chr8_1:71248869-71248888 None:intergenic 50.0%
!! TTGGTTTGATGGGGTTGGCT+TGG + chr8_1:71248870-71248889 None:intergenic 50.0%
!! TTGGGTTGGTTTGATGGGGT+TGG + chr8_1:71248875-71248894 None:intergenic 50.0%
CCCCATCAAACCAACCCAAA+TGG - chr8_1:71248876-71248895 Msa1211520:intron 50.0%
TAGAGGTAGGGGTGGCAAAA+CGG - chr8_1:71249260-71249279 Msa1211520:intron 50.0%
AACAGGTCAGCACGACTGAT+CGG + chr8_1:71249288-71249307 None:intergenic 50.0%
TTGAAAAAACAGGGCGGGGT+CGG - chr8_1:71249375-71249394 Msa1211520:intron 50.0%
! GATGATGATGAGAGGGATGC+TGG - chr8_1:71249711-71249730 Msa1211520:intron 50.0%
TCAACGAATCCGTTGACACC+TGG + chr8_1:71249981-71250000 None:intergenic 50.0%
!! CCTTACAAGCTTTGGGCCTT+CGG + chr8_1:71248639-71248658 None:intergenic 50.0%
GATTCCCGCCGATGCTAATT+TGG + chr8_1:71248810-71248829 None:intergenic 50.0%
TCGAACCTAAGACCTTGAGG+AGG - chr8_1:71248828-71248847 Msa1211520:intron 50.0%
!! TGGTTTGATGGGGTTGGCTT+GGG + chr8_1:71248869-71248888 None:intergenic 50.0%
!! TTGGTTTGATGGGGTTGGCT+TGG + chr8_1:71248870-71248889 None:intergenic 50.0%
!! TTGGGTTGGTTTGATGGGGT+TGG + chr8_1:71248875-71248894 None:intergenic 50.0%
CCCCATCAAACCAACCCAAA+TGG - chr8_1:71248876-71248895 Msa1211520:intron 50.0%
TAGAGGTAGGGGTGGCAAAA+CGG - chr8_1:71249260-71249279 Msa1211520:intron 50.0%
AACAGGTCAGCACGACTGAT+CGG + chr8_1:71249288-71249307 None:intergenic 50.0%
TTGAAAAAACAGGGCGGGGT+CGG - chr8_1:71249375-71249394 Msa1211520:intron 50.0%
! GATGATGATGAGAGGGATGC+TGG - chr8_1:71249711-71249730 Msa1211520:intron 50.0%
TCAACGAATCCGTTGACACC+TGG + chr8_1:71249981-71250000 None:intergenic 50.0%
TGGCAAGAACTCGGCTTAGG+CGG - chr8_1:71247718-71247737 Msa1211520:three_prime_UTR 55.0%
TGGCAAGAACTCGGCTTAGG+TGG - chr8_1:71247718-71247737 Msa1211520:three_prime_UTR 55.0%
CCGAAGGCCCAAAGCTTGTA+AGG - chr8_1:71248636-71248655 Msa1211520:intron 55.0%
CCGCCGATGCTAATTTGGGT+GGG + chr8_1:71248805-71248824 None:intergenic 55.0%
! CCCACCCAAATTAGCATCGG+CGG - chr8_1:71248802-71248821 Msa1211520:intron 55.0%
!! CCACCCAAATTAGCATCGGC+GGG - chr8_1:71248803-71248822 Msa1211520:intron 55.0%
GTGCTCCTCCTCAAGGTCTT+AGG + chr8_1:71248836-71248855 None:intergenic 55.0%
GTAGCATCTAGAGGTAGGGG+TGG - chr8_1:71249252-71249271 Msa1211520:intron 55.0%
AGAGGTAGGGGTGGCAAAAC+GGG - chr8_1:71249261-71249280 Msa1211520:intron 55.0%
TGTGCTGAACTTGTCCCCTC+CGG - chr8_1:71249330-71249349 Msa1211520:intron 55.0%
AACGAGACATGCCAACCGGA+GGG + chr8_1:71249348-71249367 None:intergenic 55.0%
AAACGAGACATGCCAACCGG+AGG + chr8_1:71249349-71249368 None:intergenic 55.0%
! CACGACACAAGTTAGTGGGC+CGG + chr8_1:71249401-71249420 None:intergenic 55.0%
AGTCAGGATCCGTTGACACC+AGG - chr8_1:71249960-71249979 Msa1211520:CDS 55.0%
! CCGTTGACACCTGGTGTCAA+CGG + chr8_1:71249972-71249991 None:intergenic 55.0%
CCGTTGACACCAGGTGTCAA+CGG - chr8_1:71249969-71249988 Msa1211520:CDS 55.0%
TGGCAAGAACTCGGCTTAGG+CGG - chr8_1:71247718-71247737 Msa1211520:three_prime_UTR 55.0%
TGGCAAGAACTCGGCTTAGG+TGG - chr8_1:71247718-71247737 Msa1211520:three_prime_UTR 55.0%
CCGAAGGCCCAAAGCTTGTA+AGG - chr8_1:71248636-71248655 Msa1211520:intron 55.0%
CCGCCGATGCTAATTTGGGT+GGG + chr8_1:71248805-71248824 None:intergenic 55.0%
! CCCACCCAAATTAGCATCGG+CGG - chr8_1:71248802-71248821 Msa1211520:intron 55.0%
!! CCACCCAAATTAGCATCGGC+GGG - chr8_1:71248803-71248822 Msa1211520:intron 55.0%
GTGCTCCTCCTCAAGGTCTT+AGG + chr8_1:71248836-71248855 None:intergenic 55.0%
GTAGCATCTAGAGGTAGGGG+TGG - chr8_1:71249252-71249271 Msa1211520:intron 55.0%
AGAGGTAGGGGTGGCAAAAC+GGG - chr8_1:71249261-71249280 Msa1211520:intron 55.0%
TGTGCTGAACTTGTCCCCTC+CGG - chr8_1:71249330-71249349 Msa1211520:intron 55.0%
AACGAGACATGCCAACCGGA+GGG + chr8_1:71249348-71249367 None:intergenic 55.0%
AAACGAGACATGCCAACCGG+AGG + chr8_1:71249349-71249368 None:intergenic 55.0%
! CACGACACAAGTTAGTGGGC+CGG + chr8_1:71249401-71249420 None:intergenic 55.0%
AGTCAGGATCCGTTGACACC+AGG - chr8_1:71249960-71249979 Msa1211520:CDS 55.0%
! CCGTTGACACCTGGTGTCAA+CGG + chr8_1:71249972-71249991 None:intergenic 55.0%
CCGTTGACACCAGGTGTCAA+CGG - chr8_1:71249969-71249988 Msa1211520:CDS 55.0%
CCCGCCGATGCTAATTTGGG+TGG + chr8_1:71248806-71248825 None:intergenic 60.0%
! TGGGAGTGTGCTCCTCCTCA+AGG + chr8_1:71248843-71248862 None:intergenic 60.0%
! TTGAGGAGGAGCACACTCCC+AGG - chr8_1:71248842-71248861 Msa1211520:intron 60.0%
!! ATGGGGTTGGCTTGGGACCT+GGG + chr8_1:71248862-71248881 None:intergenic 60.0%
CTGAACTTGTCCCCTCCGGT+TGG - chr8_1:71249334-71249353 Msa1211520:intron 60.0%
ACGAGACATGCCAACCGGAG+GGG + chr8_1:71249347-71249366 None:intergenic 60.0%
CCCGCCGATGCTAATTTGGG+TGG + chr8_1:71248806-71248825 None:intergenic 60.0%
! TGGGAGTGTGCTCCTCCTCA+AGG + chr8_1:71248843-71248862 None:intergenic 60.0%
! TTGAGGAGGAGCACACTCCC+AGG - chr8_1:71248842-71248861 Msa1211520:intron 60.0%
!! ATGGGGTTGGCTTGGGACCT+GGG + chr8_1:71248862-71248881 None:intergenic 60.0%
CTGAACTTGTCCCCTCCGGT+TGG - chr8_1:71249334-71249353 Msa1211520:intron 60.0%
ACGAGACATGCCAACCGGAG+GGG + chr8_1:71249347-71249366 None:intergenic 60.0%
!! GATGGGGTTGGCTTGGGACC+TGG + chr8_1:71248863-71248882 None:intergenic 65.0%
AAAAACAGGGCGGGGTCGGC+CGG - chr8_1:71249379-71249398 Msa1211520:intron 65.0%
!! GATGGGGTTGGCTTGGGACC+TGG + chr8_1:71248863-71248882 None:intergenic 65.0%
AAAAACAGGGCGGGGTCGGC+CGG - chr8_1:71249379-71249398 Msa1211520:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71247717 71250013 71247717 ID=Msa1211520;Name=Msa1211520
chr8_1 mRNA 71247717 71250013 71247717 ID=Msa1211520-mRNA-1;Parent=Msa1211520;Name=Msa1211520-mRNA-1;_AED=0.62;_eAED=0.68;_QI=0|0|0.5|0.75|1|1|4|222|168
chr8_1 exon 71247717 71248033 71247717 ID=Msa1211520-mRNA-1:exon:23912;Parent=Msa1211520-mRNA-1
chr8_1 exon 71248379 71248541 71248379 ID=Msa1211520-mRNA-1:exon:23911;Parent=Msa1211520-mRNA-1
chr8_1 exon 71249073 71249144 71249073 ID=Msa1211520-mRNA-1:exon:23910;Parent=Msa1211520-mRNA-1
chr8_1 exon 71249837 71250013 71249837 ID=Msa1211520-mRNA-1:exon:23909;Parent=Msa1211520-mRNA-1
chr8_1 CDS 71249837 71250013 71249837 ID=Msa1211520-mRNA-1:cds;Parent=Msa1211520-mRNA-1
chr8_1 CDS 71249073 71249144 71249073 ID=Msa1211520-mRNA-1:cds;Parent=Msa1211520-mRNA-1
chr8_1 CDS 71248379 71248541 71248379 ID=Msa1211520-mRNA-1:cds;Parent=Msa1211520-mRNA-1
chr8_1 CDS 71247939 71248033 71247939 ID=Msa1211520-mRNA-1:cds;Parent=Msa1211520-mRNA-1
chr8_1 three_prime_UTR 71247717 71247938 71247717 ID=Msa1211520-mRNA-1:three_prime_utr;Parent=Msa1211520-mRNA-1
Gene Sequence

>Msa1211520

ATGGCAAGAACTCGGCTTAGGCGGTCTGAGCATATAGAGAAAAGACATGTATATTCTCTAGGAAGGAGAGTAGATCAGATGGAGAGTAGTCAAATCACTGGAGGCAGTAGAAGACCTCGAAAACTTCAGCCACGTAACTTATTTTTGTTGCTGTCACAGTTATTAGTAGCTCATCTTGCTAAAACACAGTCAAAAGAAAAACAAAAGAAAAAGGAAGCAGAAACTATCCGAAGGCCCAAAGCTTGTAAGTTTGAGCAGGCTGTAATTGCTAATAACACCGGCTATGATGAGATTCTTGATGTTCATAGCTTAGTAGCATCTAGAGGTAGGGGTGGCAAAACGGGTCCGATCAGTCGTGCTGACCTGTTTAGCCCGCTATTTTTGGCGAGTTGTGCTGAACTTGTCCCCTCCGATGTAGTGGGCAATGATGATGATGAGAGGGATGCTGGAAGCTTGAAGCCAAATTATTACGACAACAACATGATCATATGTTCATGCGTTAGATGA
Protein sequence

>Msa1211520

MARTRLRRSEHIEKRHVYSLGRRVDQMESSQITGGSRRPRKLQPRNLFLLLSQLLVAHLAKTQSKEKQKKKEAETIRRPKACKFEQAVIANNTGYDEILDVHSLVASRGRGGKTGPISRADLFSPLFLASCAELVPSDVVGNDDDERDAGSLKPNYYDNNMIICSCVR*