AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417200 MtrunA17_Chr5g0404751 66.667 129 3 1 1 89 1 129 3.34e-52 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417200 AT1G77370.1 46.667 120 24 1 10 89 11 130 6.81e-26 93.2
Msa1417200 AT1G77370.2 80.000 35 7 0 55 89 122 156 1.26e-13 62.4
Msa1417200 AT1G77370.2 68.293 41 13 0 14 54 15 55 9.52e-11 55.1

Find 36 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCGTTGTCATTGTTAAT+TGG 0.231844 tig0024455:-729 Msa1417200:three_prime_UTR
AAATCAGTGTCGTGTCAAAT+TGG 0.237203 tig0024455:-2983 Msa1417200:five_prime_UTR
GGTTCCATTCTCAAGCAAAA+AGG 0.276134 tig0024455:+2854 None:intergenic
GTCCCACAAGTGTTTGTCTA+TGG 0.290060 tig0024455:-1867 Msa1417200:CDS
ATTTGACATTGGTTCTTTGT+AGG 0.328186 tig0024455:+533 None:intergenic
GACACGCGTGGAAAACAATA+AGG 0.342799 tig0024455:-3023 None:intergenic
TACGTACGGGCAATAGGATT+TGG 0.352701 tig0024455:+2764 None:intergenic
TATATATACGTACGGGCAAT+AGG 0.386425 tig0024455:+2758 None:intergenic
GGAAACTGAATTTGAAGCTT+CGG 0.429054 tig0024455:+2833 None:intergenic
AATCAGTGTCGTGTCAAATT+GGG 0.440854 tig0024455:-2982 Msa1417200:five_prime_UTR
AGCAGTGGAGCTCTAATTGT+TGG 0.456983 tig0024455:-757 Msa1417200:three_prime_UTR
AACAGAACTATTCTATTCAA+TGG 0.462801 tig0024455:+488 None:intergenic
TCCCATAGACAAACACTTGT+GGG 0.486540 tig0024455:+1865 None:intergenic
ATCTATAATTTGTATGAATG+TGG 0.490361 tig0024455:-954 Msa1417200:three_prime_UTR
TCTATAATTTGTATGAATGT+GGG 0.491540 tig0024455:-953 Msa1417200:three_prime_UTR
ATTGAATCTATCTCAAGTAA+TGG 0.497398 tig0024455:-585 Msa1417200:three_prime_UTR
TTTGTCTATGGGAAGCATAT+TGG 0.501883 tig0024455:-1855 Msa1417200:CDS
CGTGGAAAACAATAAGGGTC+TGG 0.507148 tig0024455:-3017 None:intergenic
GTCTATGGGAAGCATATTGG+TGG 0.513655 tig0024455:-1852 Msa1417200:CDS
GGAGAAGATGGTGATTCTGT+TGG 0.515760 tig0024455:+2785 None:intergenic
GCAATAGGATTTGGAGAAGA+TGG 0.516584 tig0024455:+2773 None:intergenic
TCCCACAAGTGTTTGTCTAT+GGG 0.535106 tig0024455:-1866 Msa1417200:CDS
AATCAAAGAAGTAAAACTAC+AGG 0.537670 tig0024455:-556 Msa1417200:three_prime_UTR
ACACGCGTGGAAAACAATAA+GGG 0.548482 tig0024455:-3022 None:intergenic
AAGCATATTGGTGGATCAGA+TGG 0.559694 tig0024455:-1843 Msa1417200:intron
GTTTCTGCAATTCACCACTC+TGG 0.562376 tig0024455:+1023 None:intergenic
AAGACAAGACAATTTGACAT+TGG 0.563313 tig0024455:+522 None:intergenic
CTTCGATATACATGAAAATG+CGG 0.575457 tig0024455:+926 None:intergenic
CTATAATTTGTATGAATGTG+GGG 0.594049 tig0024455:-952 Msa1417200:three_prime_UTR
TTCGATATACATGAAAATGC+GGG 0.595061 tig0024455:+927 None:intergenic
CGAAGGTTTCAAGCTTCTAG+CGG 0.615435 tig0024455:-908 Msa1417200:three_prime_UTR
TTCCCATAGACAAACACTTG+TGG 0.621034 tig0024455:+1864 None:intergenic
TGATTGTATACTTGAGACTG+AGG 0.636878 tig0024455:-986 Msa1417200:three_prime_UTR
GAAAGTACGATCATGAGCAG+TGG 0.651793 tig0024455:-772 Msa1417200:three_prime_UTR
GAAGAATGAAGATGTTACAG+TGG 0.663384 tig0024455:-2914 Msa1417200:exon
CTCAGTGCTGCAGTCCAGAG+TGG 0.673667 tig0024455:-1037 Msa1417200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTGTATTTATTTTTTA+TGG + tig0024455:2992-3011 None:intergenic 10.0%
!!! TATATGTGTTTGATATTTTA+AGG - tig0024455:1023-1042 Msa1417200:CDS 15.0%
!! AATAATCTATATACATAAGA+GGG + tig0024455:1119-1138 None:intergenic 15.0%
!! TAATAATCTATATACATAAG+AGG + tig0024455:1120-1139 None:intergenic 15.0%
!!! AAGGGAATATTTATTATTTT+CGG + tig0024455:2129-2148 None:intergenic 15.0%
!!! TTTTTTTCCATATACTTTAA+AGG - tig0024455:2220-2239 Msa1417200:intron 15.0%
!! TATCTATATGTCATTATAAA+AGG - tig0024455:2302-2321 Msa1417200:intron 15.0%
!!! AATACTTTTTCATGTTTTTT+AGG - tig0024455:2544-2563 Msa1417200:intron 15.0%
!!! ATTAAGTTGTTTATCAAACA+AGG + tig0024455:889-908 None:intergenic 20.0%
!! AATAACCTATATACATAAGA+GGG + tig0024455:1204-1223 None:intergenic 20.0%
!! AAATAACCTATATACATAAG+AGG + tig0024455:1205-1224 None:intergenic 20.0%
!! AAAAAAAAAAACTGCTTTCA+TGG - tig0024455:1306-1325 Msa1417200:intron 20.0%
!! TCATATAGTTTAAGAACTAA+AGG + tig0024455:1983-2002 None:intergenic 20.0%
!! TAAACTATATGAAAATAACG+AGG - tig0024455:1991-2010 Msa1417200:intron 20.0%
!!! TGATAGAAAATTTTTATGAG+AGG - tig0024455:2095-2114 Msa1417200:intron 20.0%
!!! TATTTAGCCTTTAAAGTATA+TGG + tig0024455:2230-2249 None:intergenic 20.0%
!! TATGTCATTATAAAAGGTTA+AGG - tig0024455:2308-2327 Msa1417200:intron 20.0%
!!! ATCTATAATTTGTATGAATG+TGG - tig0024455:2472-2491 Msa1417200:intron 20.0%
!!! TCTATAATTTGTATGAATGT+GGG - tig0024455:2473-2492 Msa1417200:intron 20.0%
!!! ATGTTTTTTAGGTATTTACT+AGG - tig0024455:2555-2574 Msa1417200:intron 20.0%
!!! ATGTTTTCATTTTGTTCTAT+AGG - tig0024455:2632-2651 Msa1417200:intron 20.0%
!!! AATTGTAGTTTTCAATAATC+TGG - tig0024455:2725-2744 Msa1417200:intron 20.0%
! AAGAGAGTTATATATACGTA+CGG + tig0024455:679-698 None:intergenic 25.0%
! TTCAATTATTATCACGTGTT+AGG - tig0024455:704-723 Msa1417200:three_prime_UTR 25.0%
! TCAATTATTATCACGTGTTA+GGG - tig0024455:705-724 Msa1417200:three_prime_UTR 25.0%
! ATGAAATTAATATGCTCTGT+CGG - tig0024455:936-955 Msa1417200:three_prime_UTR 25.0%
! TGTGTAAAATAATTGTACAC+CGG - tig0024455:1061-1080 Msa1417200:intron 25.0%
! ATTCAAATAGTATACTTAGC+CGG + tig0024455:1083-1102 None:intergenic 25.0%
! TGTGTAAAATAATTGTACAC+CGG - tig0024455:1061-1080 Msa1417200:intron 25.0%
! ATTCAAATAGTATACTTAGC+CGG + tig0024455:1083-1102 None:intergenic 25.0%
! AATGATAACAGTTGTCTATT+TGG + tig0024455:1439-1458 None:intergenic 25.0%
!! TTAAGTTGTTTATCCAAACA+AGG + tig0024455:1709-1728 None:intergenic 25.0%
! TATGAAAATAACGAGGAATT+TGG - tig0024455:1998-2017 Msa1417200:intron 25.0%
!!! CCTTATATTTTGGATATAGA+GGG + tig0024455:2045-2064 None:intergenic 25.0%
! CCTCTATATCCAAAATATAA+GGG - tig0024455:2043-2062 Msa1417200:intron 25.0%
!! ACAAATTTTCACATACTCAA+GGG + tig0024455:2147-2166 None:intergenic 25.0%
!! ATACTTTAAAGGCTAAATAG+AGG - tig0024455:2231-2250 Msa1417200:intron 25.0%
! TAATGAAGGGATTAAATTGA+AGG - tig0024455:2263-2282 Msa1417200:intron 25.0%
!! CTATAATTTGTATGAATGTG+GGG - tig0024455:2474-2493 Msa1417200:intron 25.0%
! ATTTCGTTGTCATTGTTAAT+TGG - tig0024455:2697-2716 Msa1417200:intron 25.0%
! ATTGAATCTATCTCAAGTAA+TGG - tig0024455:2841-2860 Msa1417200:CDS 25.0%
! AATCAAAGAAGTAAAACTAC+AGG - tig0024455:2870-2889 Msa1417200:CDS 25.0%
! AACAGAACTATTCTATTCAA+TGG + tig0024455:2941-2960 None:intergenic 25.0%
!!! TGTTTTTGTACCAAACAAAA+TGG - tig0024455:2957-2976 Msa1417200:five_prime_UTR 25.0%
AGAGAGTTATATATACGTAC+GGG + tig0024455:678-697 None:intergenic 30.0%
! AATGTTTCTCTGATTTTCGT+AGG - tig0024455:746-765 Msa1417200:three_prime_UTR 30.0%
AAATCAAGCTCAACAACAAA+AGG + tig0024455:817-836 None:intergenic 30.0%
!! TATATACATAAGAGGGTATC+AGG + tig0024455:1112-1131 None:intergenic 30.0%
!! TATATACATAAGAGGGTATC+AGG + tig0024455:1112-1131 None:intergenic 30.0%
TGATACCCTCTTATGTATAT+AGG - tig0024455:1111-1130 Msa1417200:intron 30.0%
!! AATTGTTTTGCGTAAGCTAT+AGG - tig0024455:1240-1259 Msa1417200:intron 30.0%
TAACAGTTGTCTATTTGGAT+TGG + tig0024455:1434-1453 None:intergenic 30.0%
! TTTTGTGCTTGTTTAGATGA+TGG - tig0024455:1490-1509 Msa1417200:intron 30.0%
TTTGTGCTTGTTTAGATGAT+GGG - tig0024455:1491-1510 Msa1417200:intron 30.0%
!! TTCTTAGACTTTTGCTTTTC+TGG - tig0024455:1617-1636 Msa1417200:intron 30.0%
!! TCTTAGACTTTTGCTTTTCT+GGG - tig0024455:1618-1637 Msa1417200:intron 30.0%
TTTCATACTTAGTCCTTGTT+TGG - tig0024455:1693-1712 Msa1417200:intron 30.0%
CCCTCTATATCCAAAATATA+AGG - tig0024455:2042-2061 Msa1417200:intron 30.0%
!! CCCTTATATTTTGGATATAG+AGG + tig0024455:2046-2065 None:intergenic 30.0%
CTATATCCAAAATATAAGGG+TGG - tig0024455:2046-2065 Msa1417200:intron 30.0%
! GACAAATTTTCACATACTCA+AGG + tig0024455:2148-2167 None:intergenic 30.0%
CATTAGTACTTAAGATGCTA+GGG + tig0024455:2179-2198 None:intergenic 30.0%
TCATTAGTACTTAAGATGCT+AGG + tig0024455:2180-2199 None:intergenic 30.0%
TCTTAAGTACTAATGAGACA+AGG - tig0024455:2184-2203 Msa1417200:intron 30.0%
CTTAAGTACTAATGAGACAA+GGG - tig0024455:2185-2204 Msa1417200:intron 30.0%
TTAAGTACTAATGAGACAAG+GGG - tig0024455:2186-2205 Msa1417200:intron 30.0%
! AGAGGAGATAATGATAATGA+AGG - tig0024455:2249-2268 Msa1417200:intron 30.0%
! GAGGAGATAATGATAATGAA+GGG - tig0024455:2250-2269 Msa1417200:intron 30.0%
TAGATAGAGAAACACAAAGA+GGG + tig0024455:2335-2354 None:intergenic 30.0%
ATAGATAGAGAAACACAAAG+AGG + tig0024455:2336-2355 None:intergenic 30.0%
TTCGATATACATGAAAATGC+GGG + tig0024455:2502-2521 None:intergenic 30.0%
CTTCGATATACATGAAAATG+CGG + tig0024455:2503-2522 None:intergenic 30.0%
!! ATTTGACATTGGTTCTTTGT+AGG + tig0024455:2896-2915 None:intergenic 30.0%
AAGACAAGACAATTTGACAT+TGG + tig0024455:2907-2926 None:intergenic 30.0%
!! GTACAAAAAGCCATTTTGTT+TGG + tig0024455:2970-2989 None:intergenic 30.0%
AAATCAGTGTCGTGTCAAAT+TGG - tig0024455:443-462 Msa1417200:three_prime_UTR 35.0%
AATCAGTGTCGTGTCAAATT+GGG - tig0024455:444-463 Msa1417200:three_prime_UTR 35.0%
GAAGAATGAAGATGTTACAG+TGG - tig0024455:512-531 Msa1417200:three_prime_UTR 35.0%
CTCAAGCAAAAAGGGTAAAA+CGG + tig0024455:566-585 None:intergenic 35.0%
! TTACCCTTTTTGCTTGAGAA+TGG - tig0024455:568-587 Msa1417200:three_prime_UTR 35.0%
GTTCCATTCTCAAGCAAAAA+GGG + tig0024455:574-593 None:intergenic 35.0%
! GGAAACTGAATTTGAAGCTT+CGG + tig0024455:596-615 None:intergenic 35.0%
!! GATTGCGTTTTGAACAAAAG+CGG + tig0024455:617-636 None:intergenic 35.0%
TATATATACGTACGGGCAAT+AGG + tig0024455:671-690 None:intergenic 35.0%
TAACGAGACAACACAATCAT+CGG + tig0024455:985-1004 None:intergenic 35.0%
!! AGATGATGGGTATCAAATTC+AGG - tig0024455:1504-1523 Msa1417200:intron 35.0%
!! GATGATGGGTATCAAATTCA+GGG - tig0024455:1505-1524 Msa1417200:intron 35.0%
! TTTGTCTATGGGAAGCATAT+TGG - tig0024455:1571-1590 Msa1417200:intron 35.0%
! CACTTTCCACCCTTATATTT+TGG + tig0024455:2055-2074 None:intergenic 35.0%
TTAAGATGCTAGGGATTAAC+AGG + tig0024455:2170-2189 None:intergenic 35.0%
TGATTGTATACTTGAGACTG+AGG - tig0024455:2440-2459 Msa1417200:intron 35.0%
! CGCATTTTCATGTATATCGA+AGG - tig0024455:2501-2520 Msa1417200:intron 35.0%
!!! ACATGTACTTTTAGCTTGTC+TGG - tig0024455:2584-2603 Msa1417200:intron 35.0%
GGTTCCATTCTCAAGCAAAA+AGG + tig0024455:575-594 None:intergenic 40.0%
GCAATAGGATTTGGAGAAGA+TGG + tig0024455:656-675 None:intergenic 40.0%
! TCAAATTCAGGGTGTTCTTC+TGG - tig0024455:1516-1535 Msa1417200:intron 40.0%
GGTGTTCTTCTGGATTTGAT+TGG - tig0024455:1526-1545 Msa1417200:intron 40.0%
TCCCACAAGTGTTTGTCTAT+GGG - tig0024455:1560-1579 Msa1417200:intron 40.0%
TCCCATAGACAAACACTTGT+GGG + tig0024455:1564-1583 None:intergenic 40.0%
TTCCCATAGACAAACACTTG+TGG + tig0024455:1565-1584 None:intergenic 40.0%
! AAGCATATTGGTGGATCAGA+TGG - tig0024455:1583-1602 Msa1417200:intron 40.0%
GTGGAAAGTGAGAGAAGATA+GGG - tig0024455:2065-2084 Msa1417200:intron 40.0%
GGAGAAGATGGTGATTCTGT+TGG + tig0024455:644-663 None:intergenic 45.0%
TACGTACGGGCAATAGGATT+TGG + tig0024455:665-684 None:intergenic 45.0%
!! GTTGTTGAGCTTGATTTGCG+AGG - tig0024455:820-839 Msa1417200:three_prime_UTR 45.0%
ACTGCTTTCATGGTCTGTCA+TGG - tig0024455:1316-1335 Msa1417200:intron 45.0%
GTCCCACAAGTGTTTGTCTA+TGG - tig0024455:1559-1578 Msa1417200:intron 45.0%
! GTCTATGGGAAGCATATTGG+TGG - tig0024455:1574-1593 Msa1417200:intron 45.0%
AGGGATGCATGTTTGAGAGA+GGG + tig0024455:2026-2045 None:intergenic 45.0%
GGTGGAAAGTGAGAGAAGAT+AGG - tig0024455:2064-2083 Msa1417200:intron 45.0%
GTTTCTGCAATTCACCACTC+TGG + tig0024455:2406-2425 None:intergenic 45.0%
CGAAGGTTTCAAGCTTCTAG+CGG - tig0024455:2518-2537 Msa1417200:intron 45.0%
! GAAAGTACGATCATGAGCAG+TGG - tig0024455:2654-2673 Msa1417200:intron 45.0%
! AGCAGTGGAGCTCTAATTGT+TGG - tig0024455:2669-2688 Msa1417200:intron 45.0%
GAGGGATGCATGTTTGAGAG+AGG + tig0024455:2027-2046 None:intergenic 50.0%
CTCAGTGCTGCAGTCCAGAG+TGG - tig0024455:2389-2408 Msa1417200:intron 60.0%
Chromosome Type Strat End Strand Name
tig0024455 gene 415 3033 415 ID=Msa1417200;Name=Msa1417200
tig0024455 mRNA 415 3033 415 ID=Msa1417200-mRNA-1;Parent=Msa1417200;Name=Msa1417200-mRNA-1;_AED=0.21;_eAED=0.21;_QI=102|0.5|0.66|1|0|0.33|3|591|89
tig0024455 exon 415 1061 415 ID=Msa1417200-mRNA-1:exon:23319;Parent=Msa1417200-mRNA-1
tig0024455 exon 1844 1896 1844 ID=Msa1417200-mRNA-1:exon:23318;Parent=Msa1417200-mRNA-1
tig0024455 exon 2771 3033 2771 ID=Msa1417200-mRNA-1:exon:23317;Parent=Msa1417200-mRNA-1
tig0024455 five_prime_UTR 2932 3033 2932 ID=Msa1417200-mRNA-1:five_prime_utr;Parent=Msa1417200-mRNA-1
tig0024455 CDS 2771 2931 2771 ID=Msa1417200-mRNA-1:cds;Parent=Msa1417200-mRNA-1
tig0024455 CDS 1844 1896 1844 ID=Msa1417200-mRNA-1:cds;Parent=Msa1417200-mRNA-1
tig0024455 CDS 1006 1061 1006 ID=Msa1417200-mRNA-1:cds;Parent=Msa1417200-mRNA-1
tig0024455 three_prime_UTR 415 1005 415 ID=Msa1417200-mRNA-1:three_prime_utr;Parent=Msa1417200-mRNA-1
Gene Sequence

>Msa1417200

ATGAAGATGTTACAGTGGAGCATGTTCATAACTCTCACGTTATTGACCGTTTTACCCTTTTTGCTTGAGAATGGAACCGAAGCTTCAAATTCAGTTTCCGCTTTTGTTCAAAACGCAATCTACTCCAACAGAATCACCATCTTCTCCAAATCCTATTGCCCAAGAACAGTCCCACAAGTGTTTGTCTATGGGAAGCATATTGGTGGATCAGATGATCTCAGTGCTGCAGTCCAGAGTGGTGAATTGCAGAAACTTCTCAAATCAAGTTGA

Protein sequence

>Msa1417200

MKMLQWSMFITLTLLTVLPFLLENGTEASNSVSAFVQNAIYSNRITIFSKSYCPRTVPQVFVYGKHIGGSDDLSAAVQSGELQKLLKSS*