AlfalfaGEDB | Msa1183450

M. sativa cultivar zhongmu-4 / Msa1183450

KEGG: K02921
GO: -
PFAM: PF01780.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183450	XP_034932641.1	100.000	92	0	0	1	92	48	139	2.05e-59	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183450	sp\|Q9ZRS8\|RL37A_PSEMZ	97.802	91	2	0	1	91	1	91	6.61e-60	180

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006910	Msa1183450	0.804565	2.032353e-49	-8.615850e-47
Msa0074030	Msa1183450	0.801817	7.528750e-49	-8.615850e-47
Msa0116670	Msa1183450	0.836826	7.452364e-57	-8.615850e-47
Msa0614630	Msa1183450	0.824705	6.985105e-54	-8.615850e-47
Msa0621950	Msa1183450	0.830117	3.518058e-55	-8.615850e-47
Msa0639290	Msa1183450	0.821634	3.636944e-53	-8.615850e-47
Msa0688850	Msa1183450	0.819885	9.183112e-53	-8.615850e-47
Msa0712610	Msa1183450	0.800099	1.689349e-48	-8.615850e-47
Msa0729200	Msa1183450	0.808487	3.023429e-50	-8.615850e-47
Msa0769590	Msa1183450	0.813890	2.035487e-51	-8.615850e-47
Msa0786660	Msa1183450	0.823315	1.480221e-53	-8.615850e-47
Msa0806880	Msa1183450	0.818641	1.763278e-52	-8.615850e-47
Msa0817540	Msa1183450	0.808452	3.076277e-50	-8.615850e-47
Msa0142110	Msa1183450	0.801056	1.077888e-48	-8.615850e-47
Msa0167480	Msa1183450	0.833109	6.439473e-56	-8.615850e-47
Msa0173530	Msa1183450	0.836059	1.168124e-56	-8.615850e-47
Msa0219120	Msa1183450	0.808092	3.671167e-50	-8.615850e-47
Msa0221030	Msa1183450	0.842544	2.423784e-58	-8.615850e-47
Msa0369450	Msa1183450	0.801134	1.039286e-48	-8.615850e-47
Msa0443390	Msa1183450	0.837619	4.669817e-57	-8.615850e-47
Msa1019240	Msa1183450	0.825117	5.583430e-54	-8.615850e-47
Msa1019260	Msa1183450	0.831038	2.093925e-55	-8.615850e-47
Msa1088490	Msa1183450	0.803285	3.749141e-49	-8.615850e-47
Msa1104210	Msa1183450	0.842812	2.057017e-58	-8.615850e-47
Msa0258850	Msa1183450	0.815609	8.467895e-52	-8.615850e-47
Msa0262480	Msa1183450	0.801491	8.782858e-49	-8.615850e-47
Msa0289610	Msa1183450	0.804939	1.697808e-49	-8.615850e-47
Msa0298200	Msa1183450	0.817820	2.704977e-52	-8.615850e-47
Msa0300350	Msa1183450	0.827063	1.924222e-54	-8.615850e-47
Msa1166860	Msa1183450	0.813393	2.618219e-51	-8.615850e-47
Msa1166870	Msa1183450	0.834956	2.221022e-56	-8.615850e-47
Msa1183450	Msa1211770	0.815234	1.026288e-51	-8.615850e-47
Msa1183450	Msa1229320	0.876949	9.296864e-69	-8.615850e-47
Msa1183450	Msa1305050	0.842633	2.294863e-58	-8.615850e-47
Msa1183450	Msa1332250	0.820025	8.529566e-53	-8.615850e-47
Msa1183450	Msa1349570	0.834529	2.843947e-56	-8.615850e-47
Msa1183450	Msa1363330	0.817888	2.611002e-52	-8.615850e-47
Msa1183450	Msa1379760	0.803590	3.241661e-49	-8.615850e-47
Msa1183450	Msa1386600	0.816933	4.283296e-52	-8.615850e-47
Msa1183450	Msa1413500	0.825433	4.700354e-54	-8.615850e-47
Msa1183450	Msa1433310	0.848388	6.328148e-60	-8.615850e-47
Msa1183450	Msa1450280	0.820381	7.069708e-53	-8.615850e-47
Msa0526540	Msa1183450	0.819871	9.248523e-53	-8.615850e-47
Msa0534750	Msa1183450	0.810175	1.313746e-50	-8.615850e-47
Msa0576940	Msa1183450	0.833187	6.157600e-56	-8.615850e-47
Msa0844220	Msa1183450	-0.819940	8.917277e-53	-8.615850e-47
Msa0945480	Msa1183450	0.821992	3.005838e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183450	MtrunA17_Chr8g0336121	100.000	92	1	92	1	92	7.50e-63	185
Msa1183450	MtrunA17_Chr4g0055341	100.000	92	1	92	1	92	7.50e-63	185
Msa1183450	MtrunA17_Chr4g0057431	100.000	92	1	92	1	92	7.50e-63	185
Msa1183450	MtrunA17_Chr8g0336341	100.000	92	1	92	1	92	7.50e-63	185

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1183450	AT3G10950.1	93.478	92	6	0	1	92	1	92	4.50e-59	176
Msa1183450	AT3G60245.1	92.308	91	7	0	1	91	1	91	8.73e-58	172

Find 49 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCAACAATACCAGCCTTCT+TGG	0.219039	8_1:+18694227	None:intergenic
AGAGGAAGGCTGTCGGTATT+TGG	0.231684	8_1:-18692633	Msa1183450:CDS
TCCATTCAATCCACCGAAAA+TGG	0.247511	8_1:-18694382	Msa1183450:intron
GAGGAAGGCTGTCGGTATTT+GGG	0.275108	8_1:-18692632	Msa1183450:CDS
TATGGGTCTGTGACTTCTAA+TGG	0.282118	8_1:+18694499	None:intergenic
TAAATTTCAGGCACCCGATA+TGG	0.335327	8_1:-18692830	Msa1183450:intron
TCCTCAAACTAGCACCATAT+CGG	0.361347	8_1:+18692816	None:intergenic
CTATATGTTGTCTAGGGTTA+AGG	0.365835	8_1:-18692152	Msa1183450:three_prime_UTR
ATCAAATATAATCTTAAAAC+AGG	0.400478	8_1:+18692343	None:intergenic
GAGGAAGCAAATTAAGAAGA+TGG	0.413539	8_1:-18692798	Msa1183450:CDS
GCTGGTATTGTTGGCAAATA+CGG	0.422228	8_1:-18694219	Msa1183450:intron
GTTACACTTCAACTAAACTA+AGG	0.434608	8_1:+18694432	None:intergenic
ACCAAGAAGGCTGGTATTGT+TGG	0.440905	8_1:-18694228	Msa1183450:CDS
CCCGATATGGTGCTAGTTTG+AGG	0.444329	8_1:-18692817	Msa1183450:CDS
CAGTCTGCTCCCTCAACCTT+CGG	0.446744	8_1:+18692430	None:intergenic
TATTTATAAGACTGCGAAAT+GGG	0.456126	8_1:+18694476	None:intergenic
TTATTTATAAGACTGCGAAA+TGG	0.457977	8_1:+18694475	None:intergenic
ATGGGTCTGTGACTTCTAAT+GGG	0.458034	8_1:+18694500	None:intergenic
TAATACAGTATGCTGTTAAG+AGG	0.458691	8_1:-18692651	Msa1183450:intron
TGATTTGCTATATGTTGTCT+AGG	0.463453	8_1:-18692159	Msa1183450:three_prime_UTR
GAACGACTTTCTTCTAATCC+TGG	0.467465	8_1:+18692073	None:intergenic
ATAAGACTGCGAAATGGGTA+TGG	0.475388	8_1:+18694481	None:intergenic
CCTCAAACTAGCACCATATC+GGG	0.503731	8_1:+18692817	None:intergenic
GAAGCACCATCCGAAGGTTG+AGG	0.512356	8_1:-18692440	Msa1183450:CDS
TTGAATGCATGCACAATACC+AGG	0.517484	8_1:-18692091	Msa1183450:three_prime_UTR
ATTTGGGGCTGCAAAGATTG+TGG	0.525352	8_1:-18692616	Msa1183450:CDS
GATTTGCTATATGTTGTCTA+GGG	0.527970	8_1:-18692158	Msa1183450:three_prime_UTR
AATGGAAGACGAGGGAATCA+AGG	0.533008	8_1:+18694399	None:intergenic
AAGCACCATCCGAAGGTTGA+GGG	0.541427	8_1:-18692439	Msa1183450:CDS
AGGAAGGCTGTCGGTATTTG+GGG	0.543829	8_1:-18692631	Msa1183450:CDS
CTGCTCCCTCAACCTTCGGA+TGG	0.565963	8_1:+18692434	None:intergenic
TAAGACTGCGAAATGGGTAT+GGG	0.568490	8_1:+18694482	None:intergenic
GATTGTGGAAAAGTGAAAGC+TGG	0.576075	8_1:-18692601	Msa1183450:CDS
ACAGACAAAGAGAACCAAGA+AGG	0.579258	8_1:-18694241	Msa1183450:intron
ACAAAGAGAACCAAGAAGGC+TGG	0.580496	8_1:-18694237	Msa1183450:CDS
CAACTAAACTAAGGTTTGTG+AGG	0.583387	8_1:+18694441	None:intergenic
AAATTCTTCTGCGAGTTCTG+TGG	0.586791	8_1:-18692758	Msa1183450:CDS
ACAGTATGCTGTTAAGAGGA+AGG	0.590694	8_1:-18692647	Msa1183450:intron
AGACGAGGGAATCAAGGTTG+AGG	0.600033	8_1:+18694405	None:intergenic
GCTTCTCACAGTTACAGCAC+TGG	0.604667	8_1:+18692458	None:intergenic
CTTCTGCGAGTTCTGTGGAA+AGG	0.605984	8_1:-18692753	Msa1183450:intron
CTGTGAGAAGCACCATCCGA+AGG	0.653930	8_1:-18692446	Msa1183450:CDS
GCTGTTAAGAGGAAGGCTGT+CGG	0.664645	8_1:-18692640	Msa1183450:CDS
TCAAATGCTATCTAGTTACG+TGG	0.670577	8_1:-18692251	Msa1183450:three_prime_UTR
GGTGGATTGAATGGAAGACG+AGG	0.673099	8_1:+18694390	None:intergenic
ACTGATTCATCATCGTACGA+AGG	0.681141	8_1:+18692224	None:intergenic
AGGGAATCAAGGTTGAGGCA+CGG	0.684740	8_1:+18694410	None:intergenic
GTGGATTGAATGGAAGACGA+GGG	0.693984	8_1:+18694391	None:intergenic
TGTGGAAAAGTGAAAGCTGG+CGG	0.773884	8_1:-18692598	Msa1183450:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATATTTAATTTCAACATTT+TGG	-	chr8_1:18692487-18692506	Msa1183450:intron	10.0%
!!!	ATATTTAATTTCAACATTTT+GGG	-	chr8_1:18692488-18692507	Msa1183450:intron	10.0%
!!!	TTTTAAGATTATATTTGATT+TGG	-	chr8_1:18694233-18694252	Msa1183450:CDS	10.0%
!!!	TTTTTTTTTTTTTTGCTAAT+AGG	-	chr8_1:18694370-18694389	Msa1183450:intron	10.0%
!!!	AATATTTAATTTCAACATTT+TGG	-	chr8_1:18692487-18692506	Msa1183450:intron	10.0%
!!!	ATATTTAATTTCAACATTTT+GGG	-	chr8_1:18692488-18692507	Msa1183450:intron	10.0%
!!!	TTTTAAGATTATATTTGATT+TGG	-	chr8_1:18694233-18694252	Msa1183450:CDS	10.0%
!!!	TTTTTTTTTTTTTTGCTAAT+AGG	-	chr8_1:18694370-18694389	Msa1183450:intron	10.0%
!!	AAAAATAAGCTAATCAAAAA+GGG	+	chr8_1:18692554-18692573	None:intergenic	15.0%
!!	AAAAAATAAGCTAATCAAAA+AGG	+	chr8_1:18692555-18692574	None:intergenic	15.0%
!!	ATTTAGATTGAATAAATTTC+AGG	-	chr8_1:18693730-18693749	Msa1183450:intron	15.0%
!!	ATCAAATATAATCTTAAAAC+AGG	+	chr8_1:18694232-18694251	None:intergenic	15.0%
!!	AAAAATAAGCTAATCAAAAA+GGG	+	chr8_1:18692554-18692573	None:intergenic	15.0%
!!	AAAAAATAAGCTAATCAAAA+AGG	+	chr8_1:18692555-18692574	None:intergenic	15.0%
!!	ATTTAGATTGAATAAATTTC+AGG	-	chr8_1:18693730-18693749	Msa1183450:intron	15.0%
!!	ATCAAATATAATCTTAAAAC+AGG	+	chr8_1:18694232-18694251	None:intergenic	15.0%
!!!	TATTGGTTTGTTCAATTTTA+AGG	-	chr8_1:18692241-18692260	Msa1183450:three_prime_UTR	20.0%
!!!	GCTTTTATGTATTATTCATA+AGG	+	chr8_1:18692676-18692695	None:intergenic	20.0%
!!	TTCTACATTCAAACTATTAA+GGG	+	chr8_1:18692744-18692763	None:intergenic	20.0%
!!	TTTCTACATTCAAACTATTA+AGG	+	chr8_1:18692745-18692764	None:intergenic	20.0%
!!	CTTCACTTACTAAAATTTAA+AGG	+	chr8_1:18693308-18693327	None:intergenic	20.0%
!!	AAATTGTCTTGAGTTAAAAT+TGG	+	chr8_1:18693615-18693634	None:intergenic	20.0%
!!!	AAGACAATTTTGTCAAATAT+TGG	-	chr8_1:18693625-18693644	Msa1183450:intron	20.0%
!!!	AGTGATTTATTTTTTGCTTT+TGG	-	chr8_1:18694444-18694463	Msa1183450:five_prime_UTR	20.0%
!!!	TTGCTTTTGGTAAATTATTT+TGG	-	chr8_1:18694457-18694476	Msa1183450:five_prime_UTR	20.0%
!!!	TGCTTTTGGTAAATTATTTT+GGG	-	chr8_1:18694458-18694477	Msa1183450:five_prime_UTR	20.0%
!!!	TATTGGTTTGTTCAATTTTA+AGG	-	chr8_1:18692241-18692260	Msa1183450:three_prime_UTR	20.0%
!!!	GCTTTTATGTATTATTCATA+AGG	+	chr8_1:18692676-18692695	None:intergenic	20.0%
!!	TTCTACATTCAAACTATTAA+GGG	+	chr8_1:18692744-18692763	None:intergenic	20.0%
!!	TTTCTACATTCAAACTATTA+AGG	+	chr8_1:18692745-18692764	None:intergenic	20.0%
!!	CTTCACTTACTAAAATTTAA+AGG	+	chr8_1:18693308-18693327	None:intergenic	20.0%
!!	AAATTGTCTTGAGTTAAAAT+TGG	+	chr8_1:18693615-18693634	None:intergenic	20.0%
!!!	AAGACAATTTTGTCAAATAT+TGG	-	chr8_1:18693625-18693644	Msa1183450:intron	20.0%
!!!	AGTGATTTATTTTTTGCTTT+TGG	-	chr8_1:18694444-18694463	Msa1183450:five_prime_UTR	20.0%
!!!	TTGCTTTTGGTAAATTATTT+TGG	-	chr8_1:18694457-18694476	Msa1183450:five_prime_UTR	20.0%
!!!	TGCTTTTGGTAAATTATTTT+GGG	-	chr8_1:18694458-18694477	Msa1183450:five_prime_UTR	20.0%
!	TATTTATAAGACTGCGAAAT+GGG	+	chr8_1:18692099-18692118	None:intergenic	25.0%
!	TTATTTATAAGACTGCGAAA+TGG	+	chr8_1:18692100-18692119	None:intergenic	25.0%
!!!	TGTTTTTTCATAAACTACCT+TGG	-	chr8_1:18692647-18692666	Msa1183450:intron	25.0%
!!!	TTATTTATTTTGCATCAGCT+CGG	-	chr8_1:18692696-18692715	Msa1183450:intron	25.0%
!	AAAACCCTTAAAATACTAGT+GGG	-	chr8_1:18692833-18692852	Msa1183450:intron	25.0%
!!	GTCACTTAAACTGATTATTT+TGG	-	chr8_1:18693240-18693259	Msa1183450:intron	25.0%
!	CACTTACTAAAATTTAAAGG+TGG	+	chr8_1:18693305-18693324	None:intergenic	25.0%
!!!	CAATTTTAACTTCAGAACAA+CGG	-	chr8_1:18693529-18693548	Msa1183450:intron	25.0%
!!	AATTATAACAGAGCTTCTTT+TGG	-	chr8_1:18693564-18693583	Msa1183450:intron	25.0%
!	TAGACAAGAAAATAAACCAA+GGG	+	chr8_1:18694043-18694062	None:intergenic	25.0%
!	ATAGACAAGAAAATAAACCA+AGG	+	chr8_1:18694044-18694063	None:intergenic	25.0%
!	CAAAACGACAAAAATGTAAA+TGG	+	chr8_1:18694179-18694198	None:intergenic	25.0%
!!!	CATTTTTGTCGTTTTGTTTT+TGG	-	chr8_1:18694183-18694202	Msa1183450:intron	25.0%
!	TATTTATAAGACTGCGAAAT+GGG	+	chr8_1:18692099-18692118	None:intergenic	25.0%
!	TTATTTATAAGACTGCGAAA+TGG	+	chr8_1:18692100-18692119	None:intergenic	25.0%
!!!	TGTTTTTTCATAAACTACCT+TGG	-	chr8_1:18692647-18692666	Msa1183450:intron	25.0%
!!!	TTATTTATTTTGCATCAGCT+CGG	-	chr8_1:18692696-18692715	Msa1183450:intron	25.0%
!	AAAACCCTTAAAATACTAGT+GGG	-	chr8_1:18692833-18692852	Msa1183450:intron	25.0%
!!	GTCACTTAAACTGATTATTT+TGG	-	chr8_1:18693240-18693259	Msa1183450:intron	25.0%
!	CACTTACTAAAATTTAAAGG+TGG	+	chr8_1:18693305-18693324	None:intergenic	25.0%
!!!	CAATTTTAACTTCAGAACAA+CGG	-	chr8_1:18693529-18693548	Msa1183450:intron	25.0%
!!	AATTATAACAGAGCTTCTTT+TGG	-	chr8_1:18693564-18693583	Msa1183450:intron	25.0%
!	TAGACAAGAAAATAAACCAA+GGG	+	chr8_1:18694043-18694062	None:intergenic	25.0%
!	ATAGACAAGAAAATAAACCA+AGG	+	chr8_1:18694044-18694063	None:intergenic	25.0%
!	CAAAACGACAAAAATGTAAA+TGG	+	chr8_1:18694179-18694198	None:intergenic	25.0%
!!!	CATTTTTGTCGTTTTGTTTT+TGG	-	chr8_1:18694183-18694202	Msa1183450:intron	25.0%
	GTTACACTTCAACTAAACTA+AGG	+	chr8_1:18692143-18692162	None:intergenic	30.0%
	TCTCTCTCTCTAATTGTTAT+TGG	-	chr8_1:18692224-18692243	Msa1183450:three_prime_UTR	30.0%
!	ACATGAATTTAGAACAACGT+TGG	+	chr8_1:18692278-18692297	None:intergenic	30.0%
!!!	TATGTTGCTTTTCAACATTC+TGG	-	chr8_1:18692376-18692395	Msa1183450:three_prime_UTR	30.0%
!!!	ATGTTGCTTTTCAACATTCT+GGG	-	chr8_1:18692377-18692396	Msa1183450:three_prime_UTR	30.0%
!!	TTTCAACATTTTGGGTGTTT+TGG	-	chr8_1:18692496-18692515	Msa1183450:intron	30.0%
!!!	CTCATCAATTTTAAGATCTG+AGG	-	chr8_1:18692528-18692547	Msa1183450:intron	30.0%
	TATTATTCATAAGGTTGCCA+AGG	+	chr8_1:18692667-18692686	None:intergenic	30.0%
	AAACTATTAAGGGCTTCATT+TGG	+	chr8_1:18692734-18692753	None:intergenic	30.0%
!	TTGAATGTAGAAAGCTTTGT+TGG	-	chr8_1:18692752-18692771	Msa1183450:intron	30.0%
	AACCCTAACGTTAAGATATA+AGG	-	chr8_1:18692803-18692822	Msa1183450:CDS	30.0%
	ACCCTAACGTTAAGATATAA+GGG	-	chr8_1:18692804-18692823	Msa1183450:CDS	30.0%
	CAAAACCCTTAAAATACTAG+TGG	-	chr8_1:18692832-18692851	Msa1183450:intron	30.0%
!!	CTAACCCACTAGTATTTTAA+GGG	+	chr8_1:18692840-18692859	None:intergenic	30.0%
	TTCGCTAAGTAAATGAACTA+GGG	-	chr8_1:18692900-18692919	Msa1183450:intron	30.0%
	AGAACAATGTTGTCAAATAG+TGG	-	chr8_1:18693097-18693116	Msa1183450:intron	30.0%
!	TTGTCAAATAGTGGCTATTT+TGG	-	chr8_1:18693106-18693125	Msa1183450:intron	30.0%
!!	CGTTGTTCTGAAGTTAAAAT+TGG	+	chr8_1:18693531-18693550	None:intergenic	30.0%
!	TTGTCAAATATTGGCTATAG+TGG	-	chr8_1:18693634-18693653	Msa1183450:intron	30.0%
!!	ATAGTGGATCTATAGTTTAG+CGG	-	chr8_1:18693650-18693669	Msa1183450:intron	30.0%
!	TGAATTCAATCTGCTTTGTT+TGG	-	chr8_1:18693865-18693884	Msa1183450:intron	30.0%
	TAATACAGTATGCTGTTAAG+AGG	-	chr8_1:18693921-18693940	Msa1183450:intron	30.0%
	AGACAAGAAAATAAACCAAG+GGG	+	chr8_1:18694042-18694061	None:intergenic	30.0%
!!	TGATTTGCTATATGTTGTCT+AGG	-	chr8_1:18694413-18694432	Msa1183450:five_prime_UTR	30.0%
!!	GATTTGCTATATGTTGTCTA+GGG	-	chr8_1:18694414-18694433	Msa1183450:five_prime_UTR	30.0%
	GTTACACTTCAACTAAACTA+AGG	+	chr8_1:18692143-18692162	None:intergenic	30.0%
	TCTCTCTCTCTAATTGTTAT+TGG	-	chr8_1:18692224-18692243	Msa1183450:three_prime_UTR	30.0%
!	ACATGAATTTAGAACAACGT+TGG	+	chr8_1:18692278-18692297	None:intergenic	30.0%
!!!	TATGTTGCTTTTCAACATTC+TGG	-	chr8_1:18692376-18692395	Msa1183450:three_prime_UTR	30.0%
!!!	ATGTTGCTTTTCAACATTCT+GGG	-	chr8_1:18692377-18692396	Msa1183450:three_prime_UTR	30.0%
!!	TTTCAACATTTTGGGTGTTT+TGG	-	chr8_1:18692496-18692515	Msa1183450:intron	30.0%
!!!	CTCATCAATTTTAAGATCTG+AGG	-	chr8_1:18692528-18692547	Msa1183450:intron	30.0%
	TATTATTCATAAGGTTGCCA+AGG	+	chr8_1:18692667-18692686	None:intergenic	30.0%
	AAACTATTAAGGGCTTCATT+TGG	+	chr8_1:18692734-18692753	None:intergenic	30.0%
!	TTGAATGTAGAAAGCTTTGT+TGG	-	chr8_1:18692752-18692771	Msa1183450:intron	30.0%
	AACCCTAACGTTAAGATATA+AGG	-	chr8_1:18692803-18692822	Msa1183450:CDS	30.0%
	ACCCTAACGTTAAGATATAA+GGG	-	chr8_1:18692804-18692823	Msa1183450:CDS	30.0%
	CAAAACCCTTAAAATACTAG+TGG	-	chr8_1:18692832-18692851	Msa1183450:intron	30.0%
!!	CTAACCCACTAGTATTTTAA+GGG	+	chr8_1:18692840-18692859	None:intergenic	30.0%
	TTCGCTAAGTAAATGAACTA+GGG	-	chr8_1:18692900-18692919	Msa1183450:intron	30.0%
	AGAACAATGTTGTCAAATAG+TGG	-	chr8_1:18693097-18693116	Msa1183450:intron	30.0%
!	TTGTCAAATAGTGGCTATTT+TGG	-	chr8_1:18693106-18693125	Msa1183450:intron	30.0%
!!	CGTTGTTCTGAAGTTAAAAT+TGG	+	chr8_1:18693531-18693550	None:intergenic	30.0%
!	TTGTCAAATATTGGCTATAG+TGG	-	chr8_1:18693634-18693653	Msa1183450:intron	30.0%
!!	ATAGTGGATCTATAGTTTAG+CGG	-	chr8_1:18693650-18693669	Msa1183450:intron	30.0%
!	TGAATTCAATCTGCTTTGTT+TGG	-	chr8_1:18693865-18693884	Msa1183450:intron	30.0%
	TAATACAGTATGCTGTTAAG+AGG	-	chr8_1:18693921-18693940	Msa1183450:intron	30.0%
	AGACAAGAAAATAAACCAAG+GGG	+	chr8_1:18694042-18694061	None:intergenic	30.0%
!!	TGATTTGCTATATGTTGTCT+AGG	-	chr8_1:18694413-18694432	Msa1183450:five_prime_UTR	30.0%
!!	GATTTGCTATATGTTGTCTA+GGG	-	chr8_1:18694414-18694433	Msa1183450:five_prime_UTR	30.0%
	CAACTAAACTAAGGTTTGTG+AGG	+	chr8_1:18692134-18692153	None:intergenic	35.0%
	ATTAAGGGCTTCATTTGGAT+TGG	+	chr8_1:18692729-18692748	None:intergenic	35.0%
	GCCCTTATATCTTAACGTTA+GGG	+	chr8_1:18692808-18692827	None:intergenic	35.0%
	TGCCCTTATATCTTAACGTT+AGG	+	chr8_1:18692809-18692828	None:intergenic	35.0%
!	CCTAACCCACTAGTATTTTA+AGG	+	chr8_1:18692841-18692860	None:intergenic	35.0%
!!	CCTTAAAATACTAGTGGGTT+AGG	-	chr8_1:18692838-18692857	Msa1183450:intron	35.0%
	GTTCGCTAAGTAAATGAACT+AGG	-	chr8_1:18692899-18692918	Msa1183450:intron	35.0%
!	TTGCGTCACTAGGATTTTAA+GGG	+	chr8_1:18692934-18692953	None:intergenic	35.0%
!	ATTGCGTCACTAGGATTTTA+AGG	+	chr8_1:18692935-18692954	None:intergenic	35.0%
	TAGATGTAAATTGCGTCACT+AGG	+	chr8_1:18692944-18692963	None:intergenic	35.0%
	TAACTTCAGAACAACGGTTA+TGG	-	chr8_1:18693535-18693554	Msa1183450:intron	35.0%
	AACTTCAGAACAACGGTTAT+GGG	-	chr8_1:18693536-18693555	Msa1183450:intron	35.0%
	GAGGAAGCAAATTAAGAAGA+TGG	-	chr8_1:18693774-18693793	Msa1183450:intron	35.0%
	GACAAGAAAATAAACCAAGG+GGG	+	chr8_1:18694041-18694060	None:intergenic	35.0%
	AATACACAAGAACAGAAGCA+CGG	+	chr8_1:18694078-18694097	None:intergenic	35.0%
!	TGATGAACAAGAAGTTTTGC+TGG	-	chr8_1:18694261-18694280	Msa1183450:intron	35.0%
	TCAAATGCTATCTAGTTACG+TGG	-	chr8_1:18694321-18694340	Msa1183450:intron	35.0%
!	CTATATGTTGTCTAGGGTTA+AGG	-	chr8_1:18694420-18694439	Msa1183450:five_prime_UTR	35.0%
	CAACTAAACTAAGGTTTGTG+AGG	+	chr8_1:18692134-18692153	None:intergenic	35.0%
	ATTAAGGGCTTCATTTGGAT+TGG	+	chr8_1:18692729-18692748	None:intergenic	35.0%
	GCCCTTATATCTTAACGTTA+GGG	+	chr8_1:18692808-18692827	None:intergenic	35.0%
	TGCCCTTATATCTTAACGTT+AGG	+	chr8_1:18692809-18692828	None:intergenic	35.0%
!	CCTAACCCACTAGTATTTTA+AGG	+	chr8_1:18692841-18692860	None:intergenic	35.0%
!!	CCTTAAAATACTAGTGGGTT+AGG	-	chr8_1:18692838-18692857	Msa1183450:intron	35.0%
	GTTCGCTAAGTAAATGAACT+AGG	-	chr8_1:18692899-18692918	Msa1183450:intron	35.0%
!	TTGCGTCACTAGGATTTTAA+GGG	+	chr8_1:18692934-18692953	None:intergenic	35.0%
!	ATTGCGTCACTAGGATTTTA+AGG	+	chr8_1:18692935-18692954	None:intergenic	35.0%
	TAGATGTAAATTGCGTCACT+AGG	+	chr8_1:18692944-18692963	None:intergenic	35.0%
	TAACTTCAGAACAACGGTTA+TGG	-	chr8_1:18693535-18693554	Msa1183450:intron	35.0%
	AACTTCAGAACAACGGTTAT+GGG	-	chr8_1:18693536-18693555	Msa1183450:intron	35.0%
	GAGGAAGCAAATTAAGAAGA+TGG	-	chr8_1:18693774-18693793	Msa1183450:intron	35.0%
	GACAAGAAAATAAACCAAGG+GGG	+	chr8_1:18694041-18694060	None:intergenic	35.0%
	AATACACAAGAACAGAAGCA+CGG	+	chr8_1:18694078-18694097	None:intergenic	35.0%
!	TGATGAACAAGAAGTTTTGC+TGG	-	chr8_1:18694261-18694280	Msa1183450:intron	35.0%
	TCAAATGCTATCTAGTTACG+TGG	-	chr8_1:18694321-18694340	Msa1183450:intron	35.0%
!	CTATATGTTGTCTAGGGTTA+AGG	-	chr8_1:18694420-18694439	Msa1183450:five_prime_UTR	35.0%
	ATGGGTCTGTGACTTCTAAT+GGG	+	chr8_1:18692075-18692094	None:intergenic	40.0%
	TATGGGTCTGTGACTTCTAA+TGG	+	chr8_1:18692076-18692095	None:intergenic	40.0%
	TAAGACTGCGAAATGGGTAT+GGG	+	chr8_1:18692093-18692112	None:intergenic	40.0%
	ATAAGACTGCGAAATGGGTA+TGG	+	chr8_1:18692094-18692113	None:intergenic	40.0%
	TCCATTCAATCCACCGAAAA+TGG	-	chr8_1:18692190-18692209	Msa1183450:three_prime_UTR	40.0%
!!!	ACCATTTTCGGTGGATTGAA+TGG	+	chr8_1:18692194-18692213	None:intergenic	40.0%
	ACAGACAAAGAGAACCAAGA+AGG	-	chr8_1:18692331-18692350	Msa1183450:three_prime_UTR	40.0%
	GCTGGTATTGTTGGCAAATA+CGG	-	chr8_1:18692353-18692372	Msa1183450:three_prime_UTR	40.0%
	CTCAGCTAAGACGAGATTAT+GGG	-	chr8_1:18692978-18692997	Msa1183450:intron	40.0%
	TAAATTTCAGGCACCCGATA+TGG	-	chr8_1:18693742-18693761	Msa1183450:intron	40.0%
	TCCTCAAACTAGCACCATAT+CGG	+	chr8_1:18693759-18693778	None:intergenic	40.0%
	AAATTCTTCTGCGAGTTCTG+TGG	-	chr8_1:18693814-18693833	Msa1183450:intron	40.0%
	GATTGAATTCATCGTCGTAG+AGG	+	chr8_1:18693856-18693875	None:intergenic	40.0%
	GTATTAACAACATCGCGTGA+CGG	+	chr8_1:18693907-18693926	None:intergenic	40.0%
	ACAGTATGCTGTTAAGAGGA+AGG	-	chr8_1:18693925-18693944	Msa1183450:intron	40.0%
	GATTGTGGAAAAGTGAAAGC+TGG	-	chr8_1:18693971-18693990	Msa1183450:intron	40.0%
!	TTTGAATCTGTTTTCCCCCT+TGG	-	chr8_1:18694024-18694043	Msa1183450:intron	40.0%
!	ACTGATTCATCATCGTACGA+AGG	+	chr8_1:18694351-18694370	None:intergenic	40.0%
	TTGAATGCATGCACAATACC+AGG	-	chr8_1:18694481-18694500	Msa1183450:five_prime_UTR	40.0%
	GAACGACTTTCTTCTAATCC+TGG	+	chr8_1:18694502-18694521	None:intergenic	40.0%
	ATGGGTCTGTGACTTCTAAT+GGG	+	chr8_1:18692075-18692094	None:intergenic	40.0%
	TATGGGTCTGTGACTTCTAA+TGG	+	chr8_1:18692076-18692095	None:intergenic	40.0%
	TAAGACTGCGAAATGGGTAT+GGG	+	chr8_1:18692093-18692112	None:intergenic	40.0%
	ATAAGACTGCGAAATGGGTA+TGG	+	chr8_1:18692094-18692113	None:intergenic	40.0%
	TCCATTCAATCCACCGAAAA+TGG	-	chr8_1:18692190-18692209	Msa1183450:three_prime_UTR	40.0%
!!!	ACCATTTTCGGTGGATTGAA+TGG	+	chr8_1:18692194-18692213	None:intergenic	40.0%
	ACAGACAAAGAGAACCAAGA+AGG	-	chr8_1:18692331-18692350	Msa1183450:three_prime_UTR	40.0%
	GCTGGTATTGTTGGCAAATA+CGG	-	chr8_1:18692353-18692372	Msa1183450:three_prime_UTR	40.0%
	CTCAGCTAAGACGAGATTAT+GGG	-	chr8_1:18692978-18692997	Msa1183450:intron	40.0%
	TAAATTTCAGGCACCCGATA+TGG	-	chr8_1:18693742-18693761	Msa1183450:intron	40.0%
	TCCTCAAACTAGCACCATAT+CGG	+	chr8_1:18693759-18693778	None:intergenic	40.0%
	AAATTCTTCTGCGAGTTCTG+TGG	-	chr8_1:18693814-18693833	Msa1183450:intron	40.0%
	GATTGAATTCATCGTCGTAG+AGG	+	chr8_1:18693856-18693875	None:intergenic	40.0%
	GTATTAACAACATCGCGTGA+CGG	+	chr8_1:18693907-18693926	None:intergenic	40.0%
	ACAGTATGCTGTTAAGAGGA+AGG	-	chr8_1:18693925-18693944	Msa1183450:intron	40.0%
	GATTGTGGAAAAGTGAAAGC+TGG	-	chr8_1:18693971-18693990	Msa1183450:intron	40.0%
!	TTTGAATCTGTTTTCCCCCT+TGG	-	chr8_1:18694024-18694043	Msa1183450:intron	40.0%
!	ACTGATTCATCATCGTACGA+AGG	+	chr8_1:18694351-18694370	None:intergenic	40.0%
	TTGAATGCATGCACAATACC+AGG	-	chr8_1:18694481-18694500	Msa1183450:five_prime_UTR	40.0%
	GAACGACTTTCTTCTAATCC+TGG	+	chr8_1:18694502-18694521	None:intergenic	40.0%
	AATGGAAGACGAGGGAATCA+AGG	+	chr8_1:18692176-18692195	None:intergenic	45.0%
!	GTGGATTGAATGGAAGACGA+GGG	+	chr8_1:18692184-18692203	None:intergenic	45.0%
!!	GAGAGAGCACGAACCATTTT+CGG	+	chr8_1:18692206-18692225	None:intergenic	45.0%
	ACAAAGAGAACCAAGAAGGC+TGG	-	chr8_1:18692335-18692354	Msa1183450:three_prime_UTR	45.0%
	ACCAAGAAGGCTGGTATTGT+TGG	-	chr8_1:18692344-18692363	Msa1183450:three_prime_UTR	45.0%
	CCATAATCTCGTCTTAGCTG+AGG	+	chr8_1:18692980-18692999	None:intergenic	45.0%
	CCTCAGCTAAGACGAGATTA+TGG	-	chr8_1:18692977-18692996	Msa1183450:intron	45.0%
	CCTCAAACTAGCACCATATC+GGG	+	chr8_1:18693758-18693777	None:intergenic	45.0%
!	ATTTGGGGCTGCAAAGATTG+TGG	-	chr8_1:18693956-18693975	Msa1183450:intron	45.0%
	TGTGGAAAAGTGAAAGCTGG+CGG	-	chr8_1:18693974-18693993	Msa1183450:intron	45.0%
	AATGGAAGACGAGGGAATCA+AGG	+	chr8_1:18692176-18692195	None:intergenic	45.0%
!	GTGGATTGAATGGAAGACGA+GGG	+	chr8_1:18692184-18692203	None:intergenic	45.0%
!!	GAGAGAGCACGAACCATTTT+CGG	+	chr8_1:18692206-18692225	None:intergenic	45.0%
	ACAAAGAGAACCAAGAAGGC+TGG	-	chr8_1:18692335-18692354	Msa1183450:three_prime_UTR	45.0%
	ACCAAGAAGGCTGGTATTGT+TGG	-	chr8_1:18692344-18692363	Msa1183450:three_prime_UTR	45.0%
	CCATAATCTCGTCTTAGCTG+AGG	+	chr8_1:18692980-18692999	None:intergenic	45.0%
	CCTCAGCTAAGACGAGATTA+TGG	-	chr8_1:18692977-18692996	Msa1183450:intron	45.0%
	CCTCAAACTAGCACCATATC+GGG	+	chr8_1:18693758-18693777	None:intergenic	45.0%
!	ATTTGGGGCTGCAAAGATTG+TGG	-	chr8_1:18693956-18693975	Msa1183450:intron	45.0%
	TGTGGAAAAGTGAAAGCTGG+CGG	-	chr8_1:18693974-18693993	Msa1183450:intron	45.0%
!!!	AGTTTTTTTTTTTTTTTTTT+TGG	-	chr8_1:18692422-18692441	Msa1183450:CDS	5.0%
!!!	AGTTTTTTTTTTTTTTTTTT+TGG	-	chr8_1:18692422-18692441	Msa1183450:CDS	5.0%
	AGGGAATCAAGGTTGAGGCA+CGG	+	chr8_1:18692165-18692184	None:intergenic	50.0%
	AGACGAGGGAATCAAGGTTG+AGG	+	chr8_1:18692170-18692189	None:intergenic	50.0%
!!	GGTGGATTGAATGGAAGACG+AGG	+	chr8_1:18692185-18692204	None:intergenic	50.0%
!	AGAGCACGAACCATTTTCGG+TGG	+	chr8_1:18692203-18692222	None:intergenic	50.0%
	GCCAACAATACCAGCCTTCT+TGG	+	chr8_1:18692348-18692367	None:intergenic	50.0%
!	CCCGATATGGTGCTAGTTTG+AGG	-	chr8_1:18693755-18693774	Msa1183450:intron	50.0%
	CTTCTGCGAGTTCTGTGGAA+AGG	-	chr8_1:18693819-18693838	Msa1183450:intron	50.0%
	GCTGTTAAGAGGAAGGCTGT+CGG	-	chr8_1:18693932-18693951	Msa1183450:intron	50.0%
	AGAGGAAGGCTGTCGGTATT+TGG	-	chr8_1:18693939-18693958	Msa1183450:intron	50.0%
	GAGGAAGGCTGTCGGTATTT+GGG	-	chr8_1:18693940-18693959	Msa1183450:intron	50.0%
	AGGAAGGCTGTCGGTATTTG+GGG	-	chr8_1:18693941-18693960	Msa1183450:intron	50.0%
	GCTTCTCACAGTTACAGCAC+TGG	+	chr8_1:18694117-18694136	None:intergenic	50.0%
	AAGCACCATCCGAAGGTTGA+GGG	-	chr8_1:18694133-18694152	Msa1183450:intron	50.0%
	AGGGAATCAAGGTTGAGGCA+CGG	+	chr8_1:18692165-18692184	None:intergenic	50.0%
	AGACGAGGGAATCAAGGTTG+AGG	+	chr8_1:18692170-18692189	None:intergenic	50.0%
!!	GGTGGATTGAATGGAAGACG+AGG	+	chr8_1:18692185-18692204	None:intergenic	50.0%
!	AGAGCACGAACCATTTTCGG+TGG	+	chr8_1:18692203-18692222	None:intergenic	50.0%
	GCCAACAATACCAGCCTTCT+TGG	+	chr8_1:18692348-18692367	None:intergenic	50.0%
!	CCCGATATGGTGCTAGTTTG+AGG	-	chr8_1:18693755-18693774	Msa1183450:intron	50.0%
	CTTCTGCGAGTTCTGTGGAA+AGG	-	chr8_1:18693819-18693838	Msa1183450:intron	50.0%
	GCTGTTAAGAGGAAGGCTGT+CGG	-	chr8_1:18693932-18693951	Msa1183450:intron	50.0%
	AGAGGAAGGCTGTCGGTATT+TGG	-	chr8_1:18693939-18693958	Msa1183450:intron	50.0%
	GAGGAAGGCTGTCGGTATTT+GGG	-	chr8_1:18693940-18693959	Msa1183450:intron	50.0%
	AGGAAGGCTGTCGGTATTTG+GGG	-	chr8_1:18693941-18693960	Msa1183450:intron	50.0%
	GCTTCTCACAGTTACAGCAC+TGG	+	chr8_1:18694117-18694136	None:intergenic	50.0%
	AAGCACCATCCGAAGGTTGA+GGG	-	chr8_1:18694133-18694152	Msa1183450:intron	50.0%
!!	CTGTGAGAAGCACCATCCGA+AGG	-	chr8_1:18694126-18694145	Msa1183450:intron	55.0%
!	GAAGCACCATCCGAAGGTTG+AGG	-	chr8_1:18694132-18694151	Msa1183450:intron	55.0%
	CAGTCTGCTCCCTCAACCTT+CGG	+	chr8_1:18694145-18694164	None:intergenic	55.0%
!!	CTGTGAGAAGCACCATCCGA+AGG	-	chr8_1:18694126-18694145	Msa1183450:intron	55.0%
!	GAAGCACCATCCGAAGGTTG+AGG	-	chr8_1:18694132-18694151	Msa1183450:intron	55.0%
	CAGTCTGCTCCCTCAACCTT+CGG	+	chr8_1:18694145-18694164	None:intergenic	55.0%
	CTGCTCCCTCAACCTTCGGA+TGG	+	chr8_1:18694141-18694160	None:intergenic	60.0%
	CTGCTCCCTCAACCTTCGGA+TGG	+	chr8_1:18694141-18694160	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	18692071	18694523	18692071	ID=Msa1183450;Name=Msa1183450
chr8_1	mRNA	18692071	18694523	18692071	ID=Msa1183450-mRNA-1;Parent=Msa1183450;Name=Msa1183450-mRNA-1;_AED=0.02;_eAED=0.02;_QI=138\|1\|1\|1\|1\|1\|5\|351\|92
chr8_1	exon	18692071	18692485	18692071	ID=Msa1183450-mRNA-1:exon:7220;Parent=Msa1183450-mRNA-1
chr8_1	exon	18692583	18692665	18692583	ID=Msa1183450-mRNA-1:exon:7219;Parent=Msa1183450-mRNA-1
chr8_1	exon	18692754	18692842	18692754	ID=Msa1183450-mRNA-1:exon:7218;Parent=Msa1183450-mRNA-1
chr8_1	exon	18694220	18694259	18694220	ID=Msa1183450-mRNA-1:exon:7217;Parent=Msa1183450-mRNA-1
chr8_1	exon	18694383	18694523	18694383	ID=Msa1183450-mRNA-1:exon:7216;Parent=Msa1183450-mRNA-1
chr8_1	five_prime_UTR	18694386	18694523	18694386	ID=Msa1183450-mRNA-1:five_prime_utr;Parent=Msa1183450-mRNA-1
chr8_1	CDS	18694383	18694385	18694383	ID=Msa1183450-mRNA-1:cds;Parent=Msa1183450-mRNA-1
chr8_1	CDS	18694220	18694259	18694220	ID=Msa1183450-mRNA-1:cds;Parent=Msa1183450-mRNA-1
chr8_1	CDS	18692754	18692842	18692754	ID=Msa1183450-mRNA-1:cds;Parent=Msa1183450-mRNA-1
chr8_1	CDS	18692583	18692665	18692583	ID=Msa1183450-mRNA-1:cds;Parent=Msa1183450-mRNA-1
chr8_1	CDS	18692422	18692485	18692422	ID=Msa1183450-mRNA-1:cds;Parent=Msa1183450-mRNA-1
chr8_1	three_prime_UTR	18692071	18692421	18692071	ID=Msa1183450-mRNA-1:three_prime_utr;Parent=Msa1183450-mRNA-1

Gene Sequence	>Msa1183450 ATGACAAAGAGAACCAAGAAGGCTGGTATTGTTGGCAAATACGGCACCCGATATGGTGCTAGTTTGAGGAAGCAAATTAAGAAGATGGAAGTCAGTCAGCACAGTAAATTCTTCTGCGAGTTCTGTGGAAAGTATGCTGTTAAGAGGAAGGCTGTCGGTATTTGGGGCTGCAAAGATTGTGGAAAAGTGAAAGCTGGCGGTGCTTACACTTTGAATACTGCCAGTGCTGTAACTGTGAGAAGCACCATCCGAAGGTTGAGGGAGCAGACTGAGAGTTAG
Protein sequence	>Msa1183450 MTKRTKKAGIVGKYGTRYGASLRKQIKKMEVSQHSKFFCEFCGKYAVKRKAVGIWGCKDCGKVKAGGAYTLNTASAVTVRSTIRRLREQTES*