AlfalfaGEDB | Msa1229830

M. sativa cultivar zhongmu-4 / Msa1229830

KEGG: K03686
GO: -
PFAM: PF16029.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229830	XP_024628414.1	92.958	71	2	1	3	70	57	127	1.66e-38	134

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014710	Msa1229830	0.809825	1.562720e-50	-8.615850e-47
Msa0017510	Msa1229830	0.839421	1.600776e-57	-8.615850e-47
Msa0110970	Msa1229830	0.807822	4.189164e-50	-8.615850e-47
Msa0114060	Msa1229830	0.808848	2.531949e-50	-8.615850e-47
Msa0115270	Msa1229830	0.801840	7.446594e-49	-8.615850e-47
Msa0657970	Msa1229830	0.828012	1.139005e-54	-8.615850e-47
Msa0663240	Msa1229830	0.804907	1.724497e-49	-8.615850e-47
Msa0673460	Msa1229830	0.813561	2.404653e-51	-8.615850e-47
Msa0688480	Msa1229830	0.812550	4.006235e-51	-8.615850e-47
Msa0743720	Msa1229830	0.831073	2.052606e-55	-8.615850e-47
Msa0745790	Msa1229830	0.802548	5.325410e-49	-8.615850e-47
Msa0822870	Msa1229830	0.811040	8.539667e-51	-8.615850e-47
Msa0140780	Msa1229830	0.810508	1.113161e-50	-8.615850e-47
Msa0157290	Msa1229830	0.838446	2.861439e-57	-8.615850e-47
Msa0200260	Msa1229830	0.820672	6.062472e-53	-8.615850e-47
Msa1226660	Msa1229830	0.800953	1.131322e-48	-8.615850e-47
Msa1228590	Msa1229830	0.812199	4.781612e-51	-8.615850e-47
Msa1229830	Msa1233890	0.801706	7.934167e-49	-8.615850e-47
Msa1229830	Msa1244250	0.802788	4.751818e-49	-8.615850e-47
Msa1229830	Msa1275360	0.803799	2.933850e-49	-8.615850e-47
Msa1229830	Msa1283860	0.804671	1.931270e-49	-8.615850e-47
Msa1229830	Msa1308380	0.801047	1.082775e-48	-8.615850e-47
Msa1229830	Msa1324500	0.809985	1.443394e-50	-8.615850e-47
Msa0419820	Msa1229830	0.802684	4.991351e-49	-8.615850e-47
Msa0428590	Msa1229830	0.816832	4.513306e-52	-8.615850e-47
Msa0440750	Msa1229830	0.822658	2.105728e-53	-8.615850e-47
Msa1014540	Msa1229830	0.824122	9.577967e-54	-8.615850e-47
Msa1082500	Msa1229830	0.819840	9.399253e-53	-8.615850e-47
Msa1085200	Msa1229830	0.801248	9.848283e-49	-8.615850e-47
Msa0243100	Msa1229830	0.808999	2.349777e-50	-8.615850e-47
Msa0259370	Msa1229830	0.802327	5.912546e-49	-8.615850e-47
Msa0287650	Msa1229830	0.812877	3.398303e-51	-8.615850e-47
Msa0345970	Msa1229830	0.805313	1.417543e-49	-8.615850e-47
Msa1151910	Msa1229830	0.806816	6.844952e-50	-8.615850e-47
Msa1186060	Msa1229830	0.830565	2.733889e-55	-8.615850e-47
Msa0507680	Msa1229830	0.800694	1.278188e-48	-8.615850e-47
Msa0871270	Msa1229830	0.802740	4.860121e-49	-8.615850e-47
Msa0880140	Msa1229830	0.843443	1.396639e-58	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229830	MtrunA17_Chr8g0337401	92.958	71	2	1	3	70	57	127	1.52e-42	134

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229830	AT5G17610.1	86.154	65	9	0	3	67	53	117	2.19e-36	118

Find 39 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTTAATCTTAAATAAAAT+TGG	0.210573	8_2:-5767299	None:intergenic
TATATTCAAATATGTGTTCT+TGG	0.265137	8_2:+5767327	Msa1229830:three_prime_UTR
GGCACTCTTCTAGCTTATAC+TGG	0.324665	8_2:-5767354	None:intergenic
AAGTTCTCTCTGTGTTGTTT+CGG	0.336010	8_2:+5767621	Msa1229830:three_prime_UTR
TGGATAGTGGATTGTGGGGT+TGG	0.367354	8_2:+5766495	Msa1229830:intron
TCCTTGGGAGAGAGCCTTAA+GGG	0.375842	8_2:+5767205	Msa1229830:CDS
CTGAATCGAAGACGAGGTTC+TGG	0.396776	8_2:+5767258	Msa1229830:three_prime_UTR
GTGTGTGGATAGTGGATTGT+GGG	0.408009	8_2:+5766490	Msa1229830:intron
TTTAACTTGCAGGTTGTCCT+TGG	0.408718	8_2:+5767189	Msa1229830:intron
GCATACAGTATTTGTACTCT+TGG	0.418223	8_2:-5766755	None:intergenic
GAGACCAAGGTATATGTTCT+TGG	0.422913	8_2:-5767462	None:intergenic
TTTGTACCTTAAGACTGATA+TGG	0.423457	8_2:+5767382	Msa1229830:three_prime_UTR
TTTGTCTATAGAAATTGTTT+TGG	0.427376	8_2:+5767656	Msa1229830:three_prime_UTR
AATCTTAAATAAAATTGGTA+AGG	0.430316	8_2:-5767294	None:intergenic
TTAACTTGCAGGTTGTCCTT+GGG	0.434808	8_2:+5767190	Msa1229830:intron
GTCCTTGGGAGAGAGCCTTA+AGG	0.442224	8_2:+5767204	Msa1229830:CDS
ATACTACCATATCAGTCTTA+AGG	0.467324	8_2:-5767388	None:intergenic
AGCGATCAAAGGCACCCTTA+AGG	0.482530	8_2:-5767219	None:intergenic
ACAGAGAGAACTTATAAGCT+TGG	0.493778	8_2:-5767611	None:intergenic
ATCTTAAATAAAATTGGTAA+GGG	0.513212	8_2:-5767293	None:intergenic
AGTCTTAAGGTACAAAACTA+AGG	0.515547	8_2:-5767375	None:intergenic
TGTGTGTGGATAGTGGATTG+TGG	0.529489	8_2:+5766489	Msa1229830:intron
TTGCATTGATTAGCGATCAA+AGG	0.534078	8_2:-5767230	None:intergenic
GCAAATCATCAATGATAGCA+AGG	0.534975	8_2:+5766522	Msa1229830:CDS
ACCCTTAAGGCTCTCTCCCA+AGG	0.561104	8_2:-5767206	None:intergenic
GCTCGTAACAAGATGGAGAA+AGG	0.580078	8_2:-5766565	None:intergenic
TAGATGAGCTCGTAACAAGA+TGG	0.581123	8_2:-5766572	None:intergenic
GAGACTAATTTATCGAAAGA+CGG	0.583749	8_2:+5767541	Msa1229830:three_prime_UTR
CAAATCATCAATGATAGCAA+GGG	0.588521	8_2:+5766523	Msa1229830:CDS
AAACTAAACTCTCGAGAGAG+AGG	0.592260	8_2:+5767580	Msa1229830:three_prime_UTR
TGCACCAAGAACATATACCT+TGG	0.614710	8_2:+5767458	Msa1229830:three_prime_UTR
GTCTCATCTCTGAGAGACCA+AGG	0.623895	8_2:-5767475	None:intergenic
CGAAAGACGGAAACACATGA+GGG	0.625160	8_2:+5767554	Msa1229830:three_prime_UTR
TCGAAAGACGGAAACACATG+AGG	0.632219	8_2:+5767553	Msa1229830:three_prime_UTR
GCTCATCTACGAGAGTGACC+CGG	0.633508	8_2:+5766586	Msa1229830:CDS
TGTGTGGATAGTGGATTGTG+GGG	0.653066	8_2:+5766491	Msa1229830:intron
ATATCTCTCAAATGCAACTG+TGG	0.656243	8_2:+5767519	Msa1229830:three_prime_UTR
AAACTTCTGAATCGAAGACG+AGG	0.708650	8_2:+5767252	Msa1229830:three_prime_UTR
GAAAGACGGAAACACATGAG+GGG	0.742834	8_2:+5767555	Msa1229830:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTTAATCTTAAATAAAAT+TGG	-	chr8_2:5767302-5767321	None:intergenic	10.0%
!!	AGTTTAATCTTAAATAAAAT+TGG	-	chr8_2:5767302-5767321	None:intergenic	10.0%
!!	ATCTTAAATAAAATTGGTAA+GGG	-	chr8_2:5767296-5767315	None:intergenic	15.0%
!!	AATCTTAAATAAAATTGGTA+AGG	-	chr8_2:5767297-5767316	None:intergenic	15.0%
!!	ATCTTAAATAAAATTGGTAA+GGG	-	chr8_2:5767296-5767315	None:intergenic	15.0%
!!	AATCTTAAATAAAATTGGTA+AGG	-	chr8_2:5767297-5767316	None:intergenic	15.0%
!!	TAATTTCAATCCCATAAAAA+AGG	-	chr8_2:5766636-5766655	None:intergenic	20.0%
!!!	GTTCTAAAGAAAAAACATTA+CGG	-	chr8_2:5767035-5767054	None:intergenic	20.0%
!!	TATATTCAAATATGTGTTCT+TGG	+	chr8_2:5767327-5767346	Msa1229830:three_prime_UTR	20.0%
!!!	TTTAAAGCAAAAAATGTACA+AGG	-	chr8_2:5767429-5767448	None:intergenic	20.0%
!!	TTTCTATAGACAAAAACTTT+GGG	-	chr8_2:5767650-5767669	None:intergenic	20.0%
!!	ATTTCTATAGACAAAAACTT+TGG	-	chr8_2:5767651-5767670	None:intergenic	20.0%
!!!	TTTGTCTATAGAAATTGTTT+TGG	+	chr8_2:5767656-5767675	Msa1229830:three_prime_UTR	20.0%
!!	TAATTTCAATCCCATAAAAA+AGG	-	chr8_2:5766636-5766655	None:intergenic	20.0%
!!!	GTTCTAAAGAAAAAACATTA+CGG	-	chr8_2:5767035-5767054	None:intergenic	20.0%
!!	TATATTCAAATATGTGTTCT+TGG	+	chr8_2:5767327-5767346	Msa1229830:three_prime_UTR	20.0%
!!!	TTTAAAGCAAAAAATGTACA+AGG	-	chr8_2:5767429-5767448	None:intergenic	20.0%
!!	TTTCTATAGACAAAAACTTT+GGG	-	chr8_2:5767650-5767669	None:intergenic	20.0%
!!	ATTTCTATAGACAAAAACTT+TGG	-	chr8_2:5767651-5767670	None:intergenic	20.0%
!!!	TTTGTCTATAGAAATTGTTT+TGG	+	chr8_2:5767656-5767675	Msa1229830:three_prime_UTR	20.0%
!!!	CATGTCATATCCTTTTTTAT+GGG	+	chr8_2:5766623-5766642	Msa1229830:intron	25.0%
!!	TTCCATAGTTCTTACATTTT+TGG	+	chr8_2:5766666-5766685	Msa1229830:intron	25.0%
!	ATCATAAATCTGTAGTAACT+TGG	-	chr8_2:5766943-5766962	None:intergenic	25.0%
!	TTATAAATGAGAAAGCAACA+TGG	+	chr8_2:5767129-5767148	Msa1229830:intron	25.0%
!!!	CATGTCATATCCTTTTTTAT+GGG	+	chr8_2:5766623-5766642	Msa1229830:intron	25.0%
!!	TTCCATAGTTCTTACATTTT+TGG	+	chr8_2:5766666-5766685	Msa1229830:intron	25.0%
!	ATCATAAATCTGTAGTAACT+TGG	-	chr8_2:5766943-5766962	None:intergenic	25.0%
!	TTATAAATGAGAAAGCAACA+TGG	+	chr8_2:5767129-5767148	Msa1229830:intron	25.0%
	TCTCCTCTTGAAAATTTGAT+TGG	+	chr8_2:5766408-5766427	Msa1229830:intron	30.0%
	CTCCTCTTGAAAATTTGATT+GGG	+	chr8_2:5766409-5766428	Msa1229830:intron	30.0%
!!	AACCCAATCAAATTTTCAAG+AGG	-	chr8_2:5766414-5766433	None:intergenic	30.0%
!!	TCTTTTCTTTTTTGTGTGTG+TGG	+	chr8_2:5766475-5766494	Msa1229830:intron	30.0%
	CAAATCATCAATGATAGCAA+GGG	+	chr8_2:5766523-5766542	Msa1229830:CDS	30.0%
!!!	GCATGTCATATCCTTTTTTA+TGG	+	chr8_2:5766622-5766641	Msa1229830:intron	30.0%
	CTCCAAAAATGTAAGAACTA+TGG	-	chr8_2:5766671-5766690	None:intergenic	30.0%
	TACAAGGAAAAAATGCAATC+TGG	-	chr8_2:5766702-5766721	None:intergenic	30.0%
	GTTCATTACATATCTATGAG+TGG	+	chr8_2:5766835-5766854	Msa1229830:intron	30.0%
	GAGTAAAGAATTGATATTGG+TGG	-	chr8_2:5766878-5766897	None:intergenic	30.0%
	GTGGAGTAAAGAATTGATAT+TGG	-	chr8_2:5766881-5766900	None:intergenic	30.0%
!!	GTATAAGTCATTCTTTTTCG+CGG	+	chr8_2:5767096-5767115	Msa1229830:intron	30.0%
!!	CATATTTGTGTTTAACTTGC+AGG	+	chr8_2:5767179-5767198	Msa1229830:intron	30.0%
	AGTCTTAAGGTACAAAACTA+AGG	-	chr8_2:5767378-5767397	None:intergenic	30.0%
	TTTGTACCTTAAGACTGATA+TGG	+	chr8_2:5767382-5767401	Msa1229830:three_prime_UTR	30.0%
	ATACTACCATATCAGTCTTA+AGG	-	chr8_2:5767391-5767410	None:intergenic	30.0%
!!!	ACTTGCTTTTTGATCTGAAT+TGG	+	chr8_2:5767496-5767515	Msa1229830:three_prime_UTR	30.0%
	GAGACTAATTTATCGAAAGA+CGG	+	chr8_2:5767541-5767560	Msa1229830:three_prime_UTR	30.0%
!!!	GTCTATAGAAATTGTTTTGG+CGG	+	chr8_2:5767659-5767678	Msa1229830:three_prime_UTR	30.0%
	TCTCCTCTTGAAAATTTGAT+TGG	+	chr8_2:5766408-5766427	Msa1229830:intron	30.0%
	CTCCTCTTGAAAATTTGATT+GGG	+	chr8_2:5766409-5766428	Msa1229830:intron	30.0%
!!	AACCCAATCAAATTTTCAAG+AGG	-	chr8_2:5766414-5766433	None:intergenic	30.0%
!!	TCTTTTCTTTTTTGTGTGTG+TGG	+	chr8_2:5766475-5766494	Msa1229830:intron	30.0%
	CAAATCATCAATGATAGCAA+GGG	+	chr8_2:5766523-5766542	Msa1229830:CDS	30.0%
!!!	GCATGTCATATCCTTTTTTA+TGG	+	chr8_2:5766622-5766641	Msa1229830:intron	30.0%
	CTCCAAAAATGTAAGAACTA+TGG	-	chr8_2:5766671-5766690	None:intergenic	30.0%
	TACAAGGAAAAAATGCAATC+TGG	-	chr8_2:5766702-5766721	None:intergenic	30.0%
	GTTCATTACATATCTATGAG+TGG	+	chr8_2:5766835-5766854	Msa1229830:intron	30.0%
	GAGTAAAGAATTGATATTGG+TGG	-	chr8_2:5766878-5766897	None:intergenic	30.0%
	GTGGAGTAAAGAATTGATAT+TGG	-	chr8_2:5766881-5766900	None:intergenic	30.0%
!!	GTATAAGTCATTCTTTTTCG+CGG	+	chr8_2:5767096-5767115	Msa1229830:intron	30.0%
!!	CATATTTGTGTTTAACTTGC+AGG	+	chr8_2:5767179-5767198	Msa1229830:intron	30.0%
	AGTCTTAAGGTACAAAACTA+AGG	-	chr8_2:5767378-5767397	None:intergenic	30.0%
	TTTGTACCTTAAGACTGATA+TGG	+	chr8_2:5767382-5767401	Msa1229830:three_prime_UTR	30.0%
	ATACTACCATATCAGTCTTA+AGG	-	chr8_2:5767391-5767410	None:intergenic	30.0%
!!!	ACTTGCTTTTTGATCTGAAT+TGG	+	chr8_2:5767496-5767515	Msa1229830:three_prime_UTR	30.0%
	GAGACTAATTTATCGAAAGA+CGG	+	chr8_2:5767541-5767560	Msa1229830:three_prime_UTR	30.0%
!!!	GTCTATAGAAATTGTTTTGG+CGG	+	chr8_2:5767659-5767678	Msa1229830:three_prime_UTR	30.0%
!	ATTGGGTTGTGTGCAATATT+TGG	+	chr8_2:5766426-5766445	Msa1229830:intron	35.0%
!!!	TTTTTTGTGTGTGTGGATAG+TGG	+	chr8_2:5766482-5766501	Msa1229830:intron	35.0%
	GCAAATCATCAATGATAGCA+AGG	+	chr8_2:5766522-5766541	Msa1229830:CDS	35.0%
	ACATGCAAAGTTTGTCTAAC+CGG	-	chr8_2:5766608-5766627	None:intergenic	35.0%
!	TTTTTCCTTGTAGCTTGAAG+AGG	+	chr8_2:5766710-5766729	Msa1229830:intron	35.0%
!	TTTTCCTTGTAGCTTGAAGA+GGG	+	chr8_2:5766711-5766730	Msa1229830:intron	35.0%
	AGGGAGAAAAAGACATGATT+CGG	+	chr8_2:5766730-5766749	Msa1229830:CDS	35.0%
	GCATACAGTATTTGTACTCT+TGG	-	chr8_2:5766758-5766777	None:intergenic	35.0%
	TTGTGGTTATGCAGAAATTG+AGG	-	chr8_2:5767004-5767023	None:intergenic	35.0%
!!!	ACATTACGGAAGTTTTGTTG+TGG	-	chr8_2:5767021-5767040	None:intergenic	35.0%
	ACATGTCTATTGCAATGAGT+TGG	+	chr8_2:5767055-5767074	Msa1229830:intron	35.0%
!!	TTGCATTGATTAGCGATCAA+AGG	-	chr8_2:5767233-5767252	None:intergenic	35.0%
	ATATCTCTCAAATGCAACTG+TGG	+	chr8_2:5767519-5767538	Msa1229830:three_prime_UTR	35.0%
	ACAGAGAGAACTTATAAGCT+TGG	-	chr8_2:5767614-5767633	None:intergenic	35.0%
!!	AAGTTCTCTCTGTGTTGTTT+CGG	+	chr8_2:5767621-5767640	Msa1229830:three_prime_UTR	35.0%
!	ATTGGGTTGTGTGCAATATT+TGG	+	chr8_2:5766426-5766445	Msa1229830:intron	35.0%
!!!	TTTTTTGTGTGTGTGGATAG+TGG	+	chr8_2:5766482-5766501	Msa1229830:intron	35.0%
	GCAAATCATCAATGATAGCA+AGG	+	chr8_2:5766522-5766541	Msa1229830:CDS	35.0%
	ACATGCAAAGTTTGTCTAAC+CGG	-	chr8_2:5766608-5766627	None:intergenic	35.0%
!	TTTTTCCTTGTAGCTTGAAG+AGG	+	chr8_2:5766710-5766729	Msa1229830:intron	35.0%
!	TTTTCCTTGTAGCTTGAAGA+GGG	+	chr8_2:5766711-5766730	Msa1229830:intron	35.0%
	AGGGAGAAAAAGACATGATT+CGG	+	chr8_2:5766730-5766749	Msa1229830:CDS	35.0%
	GCATACAGTATTTGTACTCT+TGG	-	chr8_2:5766758-5766777	None:intergenic	35.0%
	TTGTGGTTATGCAGAAATTG+AGG	-	chr8_2:5767004-5767023	None:intergenic	35.0%
!!!	ACATTACGGAAGTTTTGTTG+TGG	-	chr8_2:5767021-5767040	None:intergenic	35.0%
	ACATGTCTATTGCAATGAGT+TGG	+	chr8_2:5767055-5767074	Msa1229830:intron	35.0%
!!	TTGCATTGATTAGCGATCAA+AGG	-	chr8_2:5767233-5767252	None:intergenic	35.0%
	ATATCTCTCAAATGCAACTG+TGG	+	chr8_2:5767519-5767538	Msa1229830:three_prime_UTR	35.0%
	ACAGAGAGAACTTATAAGCT+TGG	-	chr8_2:5767614-5767633	None:intergenic	35.0%
!!	AAGTTCTCTCTGTGTTGTTT+CGG	+	chr8_2:5767621-5767640	Msa1229830:three_prime_UTR	35.0%
	TAGATGAGCTCGTAACAAGA+TGG	-	chr8_2:5766575-5766594	None:intergenic	40.0%
	CATGCAAAGTTTGTCTAACC+GGG	-	chr8_2:5766607-5766626	None:intergenic	40.0%
!	TGCAATGAGTTGGATTGACA+TGG	+	chr8_2:5767065-5767084	Msa1229830:intron	40.0%
!!	TTTAACTTGCAGGTTGTCCT+TGG	+	chr8_2:5767189-5767208	Msa1229830:intron	40.0%
!!	TTAACTTGCAGGTTGTCCTT+GGG	+	chr8_2:5767190-5767209	Msa1229830:intron	40.0%
	AAACTTCTGAATCGAAGACG+AGG	+	chr8_2:5767252-5767271	Msa1229830:three_prime_UTR	40.0%
	TGCACCAAGAACATATACCT+TGG	+	chr8_2:5767458-5767477	Msa1229830:three_prime_UTR	40.0%
	GAGACCAAGGTATATGTTCT+TGG	-	chr8_2:5767465-5767484	None:intergenic	40.0%
	AAACTAAACTCTCGAGAGAG+AGG	+	chr8_2:5767580-5767599	Msa1229830:three_prime_UTR	40.0%
	TAGATGAGCTCGTAACAAGA+TGG	-	chr8_2:5766575-5766594	None:intergenic	40.0%
	CATGCAAAGTTTGTCTAACC+GGG	-	chr8_2:5766607-5766626	None:intergenic	40.0%
!	TGCAATGAGTTGGATTGACA+TGG	+	chr8_2:5767065-5767084	Msa1229830:intron	40.0%
!!	TTTAACTTGCAGGTTGTCCT+TGG	+	chr8_2:5767189-5767208	Msa1229830:intron	40.0%
!!	TTAACTTGCAGGTTGTCCTT+GGG	+	chr8_2:5767190-5767209	Msa1229830:intron	40.0%
	AAACTTCTGAATCGAAGACG+AGG	+	chr8_2:5767252-5767271	Msa1229830:three_prime_UTR	40.0%
	TGCACCAAGAACATATACCT+TGG	+	chr8_2:5767458-5767477	Msa1229830:three_prime_UTR	40.0%
	GAGACCAAGGTATATGTTCT+TGG	-	chr8_2:5767465-5767484	None:intergenic	40.0%
	AAACTAAACTCTCGAGAGAG+AGG	+	chr8_2:5767580-5767599	Msa1229830:three_prime_UTR	40.0%
!!	TGTGTGTGGATAGTGGATTG+TGG	+	chr8_2:5766489-5766508	Msa1229830:intron	45.0%
!!	GTGTGTGGATAGTGGATTGT+GGG	+	chr8_2:5766490-5766509	Msa1229830:intron	45.0%
!!	TGTGTGGATAGTGGATTGTG+GGG	+	chr8_2:5766491-5766510	Msa1229830:intron	45.0%
	GCTCGTAACAAGATGGAGAA+AGG	-	chr8_2:5766568-5766587	None:intergenic	45.0%
	TTCTCCCTCTTCAAGCTACA+AGG	-	chr8_2:5766718-5766737	None:intergenic	45.0%
!	TATTGGTGGCTCAGTTGCTT+GGG	-	chr8_2:5766864-5766883	None:intergenic	45.0%
!	ATATTGGTGGCTCAGTTGCT+TGG	-	chr8_2:5766865-5766884	None:intergenic	45.0%
	GAACAACCTAACCACTAGGT+AGG	-	chr8_2:5766921-5766940	None:intergenic	45.0%
	CTTGGAACAACCTAACCACT+AGG	-	chr8_2:5766925-5766944	None:intergenic	45.0%
!!	GGCACTCTTCTAGCTTATAC+TGG	-	chr8_2:5767357-5767376	None:intergenic	45.0%
	TCGAAAGACGGAAACACATG+AGG	+	chr8_2:5767553-5767572	Msa1229830:three_prime_UTR	45.0%
	CGAAAGACGGAAACACATGA+GGG	+	chr8_2:5767554-5767573	Msa1229830:three_prime_UTR	45.0%
	GAAAGACGGAAACACATGAG+GGG	+	chr8_2:5767555-5767574	Msa1229830:three_prime_UTR	45.0%
!!	TGTGTGTGGATAGTGGATTG+TGG	+	chr8_2:5766489-5766508	Msa1229830:intron	45.0%
!!	GTGTGTGGATAGTGGATTGT+GGG	+	chr8_2:5766490-5766509	Msa1229830:intron	45.0%
!!	TGTGTGGATAGTGGATTGTG+GGG	+	chr8_2:5766491-5766510	Msa1229830:intron	45.0%
	GCTCGTAACAAGATGGAGAA+AGG	-	chr8_2:5766568-5766587	None:intergenic	45.0%
	TTCTCCCTCTTCAAGCTACA+AGG	-	chr8_2:5766718-5766737	None:intergenic	45.0%
!	TATTGGTGGCTCAGTTGCTT+GGG	-	chr8_2:5766864-5766883	None:intergenic	45.0%
!	ATATTGGTGGCTCAGTTGCT+TGG	-	chr8_2:5766865-5766884	None:intergenic	45.0%
	GAACAACCTAACCACTAGGT+AGG	-	chr8_2:5766921-5766940	None:intergenic	45.0%
	CTTGGAACAACCTAACCACT+AGG	-	chr8_2:5766925-5766944	None:intergenic	45.0%
!!	GGCACTCTTCTAGCTTATAC+TGG	-	chr8_2:5767357-5767376	None:intergenic	45.0%
	TCGAAAGACGGAAACACATG+AGG	+	chr8_2:5767553-5767572	Msa1229830:three_prime_UTR	45.0%
	CGAAAGACGGAAACACATGA+GGG	+	chr8_2:5767554-5767573	Msa1229830:three_prime_UTR	45.0%
	GAAAGACGGAAACACATGAG+GGG	+	chr8_2:5767555-5767574	Msa1229830:three_prime_UTR	45.0%
!!	TGGATAGTGGATTGTGGGGT+TGG	+	chr8_2:5766495-5766514	Msa1229830:intron	50.0%
!	GGTGTCGAGTTACAATCGAG+TGG	-	chr8_2:5766900-5766919	None:intergenic	50.0%
	GTAACTCGACACCTACCTAG+TGG	+	chr8_2:5766907-5766926	Msa1229830:intron	50.0%
	TCGACACCTACCTAGTGGTT+AGG	+	chr8_2:5766912-5766931	Msa1229830:intron	50.0%
	TCCTTGGGAGAGAGCCTTAA+GGG	+	chr8_2:5767205-5767224	Msa1229830:CDS	50.0%
!!	AGCGATCAAAGGCACCCTTA+AGG	-	chr8_2:5767222-5767241	None:intergenic	50.0%
	CTGAATCGAAGACGAGGTTC+TGG	+	chr8_2:5767258-5767277	Msa1229830:three_prime_UTR	50.0%
!	GTCTCATCTCTGAGAGACCA+AGG	-	chr8_2:5767478-5767497	None:intergenic	50.0%
!!	TGGATAGTGGATTGTGGGGT+TGG	+	chr8_2:5766495-5766514	Msa1229830:intron	50.0%
!	GGTGTCGAGTTACAATCGAG+TGG	-	chr8_2:5766900-5766919	None:intergenic	50.0%
	GTAACTCGACACCTACCTAG+TGG	+	chr8_2:5766907-5766926	Msa1229830:intron	50.0%
	TCGACACCTACCTAGTGGTT+AGG	+	chr8_2:5766912-5766931	Msa1229830:intron	50.0%
	TCCTTGGGAGAGAGCCTTAA+GGG	+	chr8_2:5767205-5767224	Msa1229830:CDS	50.0%
!!	AGCGATCAAAGGCACCCTTA+AGG	-	chr8_2:5767222-5767241	None:intergenic	50.0%
	CTGAATCGAAGACGAGGTTC+TGG	+	chr8_2:5767258-5767277	Msa1229830:three_prime_UTR	50.0%
!	GTCTCATCTCTGAGAGACCA+AGG	-	chr8_2:5767478-5767497	None:intergenic	50.0%
	GCTCATCTACGAGAGTGACC+CGG	+	chr8_2:5766586-5766605	Msa1229830:CDS	55.0%
	GTCCTTGGGAGAGAGCCTTA+AGG	+	chr8_2:5767204-5767223	Msa1229830:CDS	55.0%
	ACCCTTAAGGCTCTCTCCCA+AGG	-	chr8_2:5767209-5767228	None:intergenic	55.0%
	GCTCATCTACGAGAGTGACC+CGG	+	chr8_2:5766586-5766605	Msa1229830:CDS	55.0%
	GTCCTTGGGAGAGAGCCTTA+AGG	+	chr8_2:5767204-5767223	Msa1229830:CDS	55.0%
	ACCCTTAAGGCTCTCTCCCA+AGG	-	chr8_2:5767209-5767228	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	5766376	5767690	5766376	ID=Msa1229830;Name=Msa1229830
chr8_2	mRNA	5766376	5767690	5766376	ID=Msa1229830-mRNA-1;Parent=Msa1229830;Name=Msa1229830-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0.66\|0.75\|1\|0\|0\|4\|447\|70
chr8_2	exon	5766376	5766380	5766376	ID=Msa1229830-mRNA-1:exon:1982;Parent=Msa1229830-mRNA-1
chr8_2	exon	5766502	5766607	5766502	ID=Msa1229830-mRNA-1:exon:1983;Parent=Msa1229830-mRNA-1
chr8_2	exon	5766723	5766781	5766723	ID=Msa1229830-mRNA-1:exon:1984;Parent=Msa1229830-mRNA-1
chr8_2	exon	5767201	5767690	5767201	ID=Msa1229830-mRNA-1:exon:1985;Parent=Msa1229830-mRNA-1
chr8_2	CDS	5766376	5766380	5766376	ID=Msa1229830-mRNA-1:cds;Parent=Msa1229830-mRNA-1
chr8_2	CDS	5766502	5766607	5766502	ID=Msa1229830-mRNA-1:cds;Parent=Msa1229830-mRNA-1
chr8_2	CDS	5766723	5766781	5766723	ID=Msa1229830-mRNA-1:cds;Parent=Msa1229830-mRNA-1
chr8_2	CDS	5767201	5767243	5767201	ID=Msa1229830-mRNA-1:cds;Parent=Msa1229830-mRNA-1
chr8_2	three_prime_UTR	5767244	5767690	5767244	ID=Msa1229830-mRNA-1:three_prime_utr;Parent=Msa1229830-mRNA-1

Gene Sequence	>Msa1229830 ATGATTGGATTGTGGGGTTGGCAATGCAAATCATCAATGATAGCAAGGGAGAATTGTGCTTTACGATGCCTTTCTCCATCTTGTTACGAGCTCATCTACGAGAGTGACCCGCTTGAAGAGGGAGAAAAAGACATGATTCGGAGCCAAGAGTACAAATACTGTATGCTTAAGTTGTCCTTGGGAGAGAGCCTTAAGGGTGCCTTTGATCGCTAA
Protein sequence	>Msa1229830 MIGLWGWQCKSSMIARENCALRCLSPSCYELIYESDPLEEGEKDMIRSQEYKYCMLKLSLGESLKGAFDR*