AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0235050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235050 MtrunA17_Chr2g0283551 100.000 86 0 0 1 86 1 86 1.11e-60 179
Msa0235050 MtrunA17_Chr2g0291901 98.837 86 1 0 1 86 1 86 3.27e-60 177
Msa0235050 MtrunA17_Chr1g0210581 98.837 86 1 0 1 86 1 86 3.27e-60 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235050 AT3G61110.1 95.349 86 4 0 1 86 1 86 4.63e-59 175
Msa0235050 AT2G45710.1 94.048 84 5 0 1 84 1 84 3.86e-56 167
Msa0235050 AT5G47930.1 95.238 84 4 0 1 84 1 84 1.13e-55 166
Msa0235050 AT3G61111.1 82.558 86 15 0 1 86 1 86 4.94e-49 150
Msa0235050 AT3G61111.2 80.952 42 8 0 1 42 1 42 1.06e-18 73.6

Find 37 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATGCTAGGTTAAAGTTT+TGG 0.280121 2_2:+7803793 None:intergenic
TCTATTTACTGTTGTGCATC+TGG 0.299569 2_2:-7803772 Msa0235050:three_prime_UTR
GTTGCAGAGTTGTGGTTGTT+AGG 0.336511 2_2:-7803903 Msa0235050:three_prime_UTR
TAGGAAGAAGGGAGACTAAA+TGG 0.336934 2_2:-7803950 Msa0235050:exon
TAACAGAAGGATGCTCCTTT+AGG 0.360674 2_2:-7803969 Msa0235050:CDS
TTTCTCTTCTCAAGCTCAGC+TGG 0.378351 2_2:+7804500 None:intergenic
ACCGTCTTGTGCCAGCCAAC+AGG 0.416778 2_2:-7804006 Msa0235050:CDS
TGTGGTTGTTAGGCTTAAGA+TGG 0.436847 2_2:-7803893 Msa0235050:three_prime_UTR
TTAGTCTCCCTTCTTCCTAA+AGG 0.438236 2_2:+7803954 None:intergenic
CTCTTCTCAAGCTCAGCTGG+TGG 0.449604 2_2:+7804503 None:intergenic
TGTCATCTTTACTTTGTCCA+TGG 0.464001 2_2:-7803925 Msa0235050:three_prime_UTR
TTGCACGGCCACCTGTTGGC+TGG 0.466909 2_2:+7803995 None:intergenic
CAACCACAACTCTGCAACCA+TGG 0.471278 2_2:+7803908 None:intergenic
AGCCAACAGGTGGCCGTGCA+AGG 0.485723 2_2:-7803993 Msa0235050:CDS
TCTTCTCAAGCTCAGCTGGT+GGG 0.492805 2_2:+7804504 None:intergenic
AGGATGCTCCTTTAGGAAGA+AGG 0.507022 2_2:-7803962 Msa0235050:CDS
GGATGCTCCTTTAGGAAGAA+GGG 0.515173 2_2:-7803961 Msa0235050:CDS
CTTACATATTGAAACAACCT+TGG 0.524068 2_2:+7804323 None:intergenic
TGTCCATGGTTGCAGAGTTG+TGG 0.535567 2_2:-7803911 Msa0235050:three_prime_UTR
AGCCTTGCACGGCCACCTGT+TGG 0.536283 2_2:+7803991 None:intergenic
CGTGTAATCTGTCTTGCGAT+TGG 0.550265 2_2:-7803654 Msa0235050:three_prime_UTR
TTGCAGGATGTAAAATGCCA+AGG 0.553924 2_2:-7804340 Msa0235050:intron
ATCCTTCTGTTAGCCTTGCA+CGG 0.562687 2_2:+7803980 None:intergenic
CAACAGTAAATAGAAATGCT+AGG 0.572412 2_2:+7803781 None:intergenic
ACCTGTTGGCTGGCACAAGA+CGG 0.579670 2_2:+7804005 None:intergenic
TAGCCACTCTCAAACCGTTG+TGG 0.599496 2_2:-7804046 Msa0235050:CDS
CAGGGCACTGACCATGTTGG+CGG 0.602656 2_2:+7804782 None:intergenic
GTCTTGTGCCAGCCAACAGG+TGG 0.604328 2_2:-7804003 Msa0235050:CDS
ACCATGTTGGCGGAAAATGT+TGG 0.615242 2_2:+7804792 None:intergenic
GGCCGTGCAAGGCTAACAGA+AGG 0.622759 2_2:-7803982 Msa0235050:CDS
TCTCAAACCGTTGTGGTGTG+CGG 0.635679 2_2:-7804039 Msa0235050:CDS
GAACAGGGCACTGACCATGT+TGG 0.640363 2_2:+7804779 None:intergenic
ACACCACAACGGTTTGAGAG+TGG 0.647039 2_2:+7804043 None:intergenic
CGGAAAATGTTGGAAAACAG+AGG 0.656697 2_2:+7804802 None:intergenic
TTGAGAGTGGCTAAAGACAG+TGG 0.660753 2_2:+7804056 None:intergenic
TCTTGCGATTGGTGTGTCCG+AGG 0.681893 2_2:-7803643 None:intergenic
ACAGTTACCGCACACCACAA+CGG 0.704309 2_2:+7804032 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTTTATTGTTTTTTT+TGG - chr2_2:7803785-7803804 Msa0235050:three_prime_UTR 10.0%
!!! TTGATTTTTATTGTTTTTTT+TGG - chr2_2:7803785-7803804 Msa0235050:three_prime_UTR 10.0%
!!! TTTTGCAAAAATATCATTTA+TGG - chr2_2:7803760-7803779 Msa0235050:three_prime_UTR 15.0%
!! AAAAAGTTTATAAGCAAAAT+AGG + chr2_2:7804403-7804422 None:intergenic 15.0%
!!! TGCTTATAAACTTTTTATTT+TGG - chr2_2:7804407-7804426 Msa0235050:intron 15.0%
!!! TTTTGCAAAAATATCATTTA+TGG - chr2_2:7803760-7803779 Msa0235050:three_prime_UTR 15.0%
!! AAAAAGTTTATAAGCAAAAT+AGG + chr2_2:7804403-7804422 None:intergenic 15.0%
!!! TGCTTATAAACTTTTTATTT+TGG - chr2_2:7804407-7804426 Msa0235050:intron 15.0%
!!! ATCTCCTTCTGAAATTTTTA+GGG - chr2_2:7803649-7803668 Msa0235050:three_prime_UTR 25.0%
! AAAACCCTAAAAATTTCAGA+AGG + chr2_2:7803656-7803675 None:intergenic 25.0%
!!! TTCTGAAATTTTTAGGGTTT+TGG - chr2_2:7803655-7803674 Msa0235050:three_prime_UTR 25.0%
! CACAACATGAACATAAAATT+TGG + chr2_2:7803811-7803830 None:intergenic 25.0%
!!! AATCTATTTGTTGTTTGTTC+AGG - chr2_2:7803939-7803958 Msa0235050:exon 25.0%
! AATACCATGAAAAAAGAGTT+TGG + chr2_2:7804048-7804067 None:intergenic 25.0%
!!! TTGTTTTTCTCTTTTATTGC+AGG - chr2_2:7804138-7804157 Msa0235050:intron 25.0%
! ATAATAGTGCTCAAATTCAT+TGG - chr2_2:7804240-7804259 Msa0235050:intron 25.0%
!!! AAAGTTTTGGATAAGATTGT+GGG + chr2_2:7804691-7804710 None:intergenic 25.0%
!!! TAAAGTTTTGGATAAGATTG+TGG + chr2_2:7804692-7804711 None:intergenic 25.0%
!!! TCTTCATTATGTTTTGTTTG+TGG - chr2_2:7804817-7804836 Msa0235050:five_prime_UTR 25.0%
!!! ATCTCCTTCTGAAATTTTTA+GGG - chr2_2:7803649-7803668 Msa0235050:three_prime_UTR 25.0%
! AAAACCCTAAAAATTTCAGA+AGG + chr2_2:7803656-7803675 None:intergenic 25.0%
!!! TTCTGAAATTTTTAGGGTTT+TGG - chr2_2:7803655-7803674 Msa0235050:three_prime_UTR 25.0%
! CACAACATGAACATAAAATT+TGG + chr2_2:7803811-7803830 None:intergenic 25.0%
!!! AATCTATTTGTTGTTTGTTC+AGG - chr2_2:7803939-7803958 Msa0235050:exon 25.0%
! AATACCATGAAAAAAGAGTT+TGG + chr2_2:7804048-7804067 None:intergenic 25.0%
!!! TTGTTTTTCTCTTTTATTGC+AGG - chr2_2:7804138-7804157 Msa0235050:intron 25.0%
! ATAATAGTGCTCAAATTCAT+TGG - chr2_2:7804240-7804259 Msa0235050:intron 25.0%
!!! AAAGTTTTGGATAAGATTGT+GGG + chr2_2:7804691-7804710 None:intergenic 25.0%
!!! TAAAGTTTTGGATAAGATTG+TGG + chr2_2:7804692-7804711 None:intergenic 25.0%
!!! TCTTCATTATGTTTTGTTTG+TGG - chr2_2:7804817-7804836 Msa0235050:five_prime_UTR 25.0%
!!! CATCTCCTTCTGAAATTTTT+AGG - chr2_2:7803648-7803667 Msa0235050:three_prime_UTR 30.0%
CAAATTCAGATCTGAAAAAG+AGG + chr2_2:7803740-7803759 None:intergenic 30.0%
! TCATGTTGTGTTTTCATAAC+AGG - chr2_2:7803821-7803840 Msa0235050:three_prime_UTR 30.0%
CTTACATATTGAAACAACCT+TGG + chr2_2:7804174-7804193 None:intergenic 30.0%
! GGTTGTTTCAATATGTAAGT+TGG - chr2_2:7804175-7804194 Msa0235050:intron 30.0%
! GAAATGCTAGGTTAAAGTTT+TGG + chr2_2:7804704-7804723 None:intergenic 30.0%
CAACAGTAAATAGAAATGCT+AGG + chr2_2:7804716-7804735 None:intergenic 30.0%
!!! CATCTCCTTCTGAAATTTTT+AGG - chr2_2:7803648-7803667 Msa0235050:three_prime_UTR 30.0%
CAAATTCAGATCTGAAAAAG+AGG + chr2_2:7803740-7803759 None:intergenic 30.0%
! TCATGTTGTGTTTTCATAAC+AGG - chr2_2:7803821-7803840 Msa0235050:three_prime_UTR 30.0%
CTTACATATTGAAACAACCT+TGG + chr2_2:7804174-7804193 None:intergenic 30.0%
! GGTTGTTTCAATATGTAAGT+TGG - chr2_2:7804175-7804194 Msa0235050:intron 30.0%
! GAAATGCTAGGTTAAAGTTT+TGG + chr2_2:7804704-7804723 None:intergenic 30.0%
CAACAGTAAATAGAAATGCT+AGG + chr2_2:7804716-7804735 None:intergenic 30.0%
AGATCTGAAAAAGAGGAACA+GGG + chr2_2:7803733-7803752 None:intergenic 35.0%
!!! GTCACCAAACTCTTTTTTCA+TGG - chr2_2:7804041-7804060 Msa0235050:CDS 35.0%
TGTCATCTTTACTTTGTCCA+TGG - chr2_2:7804569-7804588 Msa0235050:intron 35.0%
TCTATTTACTGTTGTGCATC+TGG - chr2_2:7804722-7804741 Msa0235050:intron 35.0%
AGATCTGAAAAAGAGGAACA+GGG + chr2_2:7803733-7803752 None:intergenic 35.0%
!!! GTCACCAAACTCTTTTTTCA+TGG - chr2_2:7804041-7804060 Msa0235050:CDS 35.0%
TGTCATCTTTACTTTGTCCA+TGG - chr2_2:7804569-7804588 Msa0235050:intron 35.0%
TCTATTTACTGTTGTGCATC+TGG - chr2_2:7804722-7804741 Msa0235050:intron 35.0%
CGGAAAATGTTGGAAAACAG+AGG + chr2_2:7803695-7803714 None:intergenic 40.0%
CAGATCTGAAAAAGAGGAAC+AGG + chr2_2:7803734-7803753 None:intergenic 40.0%
TTGCAGGATGTAAAATGCCA+AGG - chr2_2:7804154-7804173 Msa0235050:intron 40.0%
TAACAGAAGGATGCTCCTTT+AGG - chr2_2:7804525-7804544 Msa0235050:CDS 40.0%
TTAGTCTCCCTTCTTCCTAA+AGG + chr2_2:7804543-7804562 None:intergenic 40.0%
TAGGAAGAAGGGAGACTAAA+TGG - chr2_2:7804544-7804563 Msa0235050:intron 40.0%
TGTGGTTGTTAGGCTTAAGA+TGG - chr2_2:7804601-7804620 Msa0235050:intron 40.0%
CGGAAAATGTTGGAAAACAG+AGG + chr2_2:7803695-7803714 None:intergenic 40.0%
CAGATCTGAAAAAGAGGAAC+AGG + chr2_2:7803734-7803753 None:intergenic 40.0%
TTGCAGGATGTAAAATGCCA+AGG - chr2_2:7804154-7804173 Msa0235050:intron 40.0%
TAACAGAAGGATGCTCCTTT+AGG - chr2_2:7804525-7804544 Msa0235050:CDS 40.0%
TTAGTCTCCCTTCTTCCTAA+AGG + chr2_2:7804543-7804562 None:intergenic 40.0%
TAGGAAGAAGGGAGACTAAA+TGG - chr2_2:7804544-7804563 Msa0235050:intron 40.0%
TGTGGTTGTTAGGCTTAAGA+TGG - chr2_2:7804601-7804620 Msa0235050:intron 40.0%
! TCCAACATTTTCCGCCAACA+TGG - chr2_2:7803701-7803720 Msa0235050:three_prime_UTR 45.0%
ACCATGTTGGCGGAAAATGT+TGG + chr2_2:7803705-7803724 None:intergenic 45.0%
TTTCTCTTCTCAAGCTCAGC+TGG + chr2_2:7803997-7804016 None:intergenic 45.0%
TTGAGAGTGGCTAAAGACAG+TGG + chr2_2:7804441-7804460 None:intergenic 45.0%
!! ATCCTTCTGTTAGCCTTGCA+CGG + chr2_2:7804517-7804536 None:intergenic 45.0%
! AGGATGCTCCTTTAGGAAGA+AGG - chr2_2:7804532-7804551 Msa0235050:CDS 45.0%
! GGATGCTCCTTTAGGAAGAA+GGG - chr2_2:7804533-7804552 Msa0235050:CDS 45.0%
GTTGCAGAGTTGTGGTTGTT+AGG - chr2_2:7804591-7804610 Msa0235050:intron 45.0%
CGTGTAATCTGTCTTGCGAT+TGG - chr2_2:7804840-7804859 Msa0235050:five_prime_UTR 45.0%
! TCCAACATTTTCCGCCAACA+TGG - chr2_2:7803701-7803720 Msa0235050:three_prime_UTR 45.0%
ACCATGTTGGCGGAAAATGT+TGG + chr2_2:7803705-7803724 None:intergenic 45.0%
TTTCTCTTCTCAAGCTCAGC+TGG + chr2_2:7803997-7804016 None:intergenic 45.0%
TTGAGAGTGGCTAAAGACAG+TGG + chr2_2:7804441-7804460 None:intergenic 45.0%
!! ATCCTTCTGTTAGCCTTGCA+CGG + chr2_2:7804517-7804536 None:intergenic 45.0%
! AGGATGCTCCTTTAGGAAGA+AGG - chr2_2:7804532-7804551 Msa0235050:CDS 45.0%
! GGATGCTCCTTTAGGAAGAA+GGG - chr2_2:7804533-7804552 Msa0235050:CDS 45.0%
GTTGCAGAGTTGTGGTTGTT+AGG - chr2_2:7804591-7804610 Msa0235050:intron 45.0%
CGTGTAATCTGTCTTGCGAT+TGG - chr2_2:7804840-7804859 Msa0235050:five_prime_UTR 45.0%
TCTTCTCAAGCTCAGCTGGT+GGG + chr2_2:7803993-7804012 None:intergenic 50.0%
TAGCCACTCTCAAACCGTTG+TGG - chr2_2:7804448-7804467 Msa0235050:intron 50.0%
ACACCACAACGGTTTGAGAG+TGG + chr2_2:7804454-7804473 None:intergenic 50.0%
TCTCAAACCGTTGTGGTGTG+CGG - chr2_2:7804455-7804474 Msa0235050:CDS 50.0%
ACAGTTACCGCACACCACAA+CGG + chr2_2:7804465-7804484 None:intergenic 50.0%
TGTCCATGGTTGCAGAGTTG+TGG - chr2_2:7804583-7804602 Msa0235050:intron 50.0%
CAACCACAACTCTGCAACCA+TGG + chr2_2:7804589-7804608 None:intergenic 50.0%
TCTTCTCAAGCTCAGCTGGT+GGG + chr2_2:7803993-7804012 None:intergenic 50.0%
TAGCCACTCTCAAACCGTTG+TGG - chr2_2:7804448-7804467 Msa0235050:intron 50.0%
ACACCACAACGGTTTGAGAG+TGG + chr2_2:7804454-7804473 None:intergenic 50.0%
TCTCAAACCGTTGTGGTGTG+CGG - chr2_2:7804455-7804474 Msa0235050:CDS 50.0%
ACAGTTACCGCACACCACAA+CGG + chr2_2:7804465-7804484 None:intergenic 50.0%
TGTCCATGGTTGCAGAGTTG+TGG - chr2_2:7804583-7804602 Msa0235050:intron 50.0%
CAACCACAACTCTGCAACCA+TGG + chr2_2:7804589-7804608 None:intergenic 50.0%
!! GAACAGGGCACTGACCATGT+TGG + chr2_2:7803718-7803737 None:intergenic 55.0%
CTCTTCTCAAGCTCAGCTGG+TGG + chr2_2:7803994-7804013 None:intergenic 55.0%
! ACCTGTTGGCTGGCACAAGA+CGG + chr2_2:7804492-7804511 None:intergenic 55.0%
!! GAACAGGGCACTGACCATGT+TGG + chr2_2:7803718-7803737 None:intergenic 55.0%
CTCTTCTCAAGCTCAGCTGG+TGG + chr2_2:7803994-7804013 None:intergenic 55.0%
! ACCTGTTGGCTGGCACAAGA+CGG + chr2_2:7804492-7804511 None:intergenic 55.0%
!! CAGGGCACTGACCATGTTGG+CGG + chr2_2:7803715-7803734 None:intergenic 60.0%
ACCGTCTTGTGCCAGCCAAC+AGG - chr2_2:7804488-7804507 Msa0235050:CDS 60.0%
GTCTTGTGCCAGCCAACAGG+TGG - chr2_2:7804491-7804510 Msa0235050:CDS 60.0%
GGCCGTGCAAGGCTAACAGA+AGG - chr2_2:7804512-7804531 Msa0235050:CDS 60.0%
!! CAGGGCACTGACCATGTTGG+CGG + chr2_2:7803715-7803734 None:intergenic 60.0%
ACCGTCTTGTGCCAGCCAAC+AGG - chr2_2:7804488-7804507 Msa0235050:CDS 60.0%
GTCTTGTGCCAGCCAACAGG+TGG - chr2_2:7804491-7804510 Msa0235050:CDS 60.0%
GGCCGTGCAAGGCTAACAGA+AGG - chr2_2:7804512-7804531 Msa0235050:CDS 60.0%
TTGCACGGCCACCTGTTGGC+TGG + chr2_2:7804502-7804521 None:intergenic 65.0%
AGCCAACAGGTGGCCGTGCA+AGG - chr2_2:7804501-7804520 Msa0235050:CDS 65.0%
AGCCTTGCACGGCCACCTGT+TGG + chr2_2:7804506-7804525 None:intergenic 65.0%
TTGCACGGCCACCTGTTGGC+TGG + chr2_2:7804502-7804521 None:intergenic 65.0%
AGCCAACAGGTGGCCGTGCA+AGG - chr2_2:7804501-7804520 Msa0235050:CDS 65.0%
AGCCTTGCACGGCCACCTGT+TGG + chr2_2:7804506-7804525 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 7803644 7804872 7803644 ID=Msa0235050;Name=Msa0235050
chr2_2 mRNA 7803644 7804872 7803644 ID=Msa0235050-mRNA-1;Parent=Msa0235050;Name=Msa0235050-mRNA-1;_AED=0.03;_eAED=0.03;_QI=76|1|1|1|1|1|4|310|86
chr2_2 exon 7803644 7804080 7803644 ID=Msa0235050-mRNA-1:exon:3450;Parent=Msa0235050-mRNA-1
chr2_2 exon 7804328 7804356 7804328 ID=Msa0235050-mRNA-1:exon:3449;Parent=Msa0235050-mRNA-1
chr2_2 exon 7804454 7804555 7804454 ID=Msa0235050-mRNA-1:exon:3448;Parent=Msa0235050-mRNA-1
chr2_2 exon 7804794 7804872 7804794 ID=Msa0235050-mRNA-1:exon:3447;Parent=Msa0235050-mRNA-1
chr2_2 five_prime_UTR 7804797 7804872 7804797 ID=Msa0235050-mRNA-1:five_prime_utr;Parent=Msa0235050-mRNA-1
chr2_2 CDS 7804794 7804796 7804794 ID=Msa0235050-mRNA-1:cds;Parent=Msa0235050-mRNA-1
chr2_2 CDS 7804454 7804555 7804454 ID=Msa0235050-mRNA-1:cds;Parent=Msa0235050-mRNA-1
chr2_2 CDS 7804328 7804356 7804328 ID=Msa0235050-mRNA-1:cds;Parent=Msa0235050-mRNA-1
chr2_2 CDS 7803954 7804080 7803954 ID=Msa0235050-mRNA-1:cds;Parent=Msa0235050-mRNA-1
chr2_2 three_prime_UTR 7803644 7803953 7803644 ID=Msa0235050-mRNA-1:three_prime_utr;Parent=Msa0235050-mRNA-1
Gene Sequence

>Msa0235050

ATGGTTCTTCAAAATGATATTGATTTGTTAAACCCACCAGCTGAGCTTGAGAAGAGAAAGCACAAGCTCAAAAGACTTGTTCAGTCACCAAACTCTTTTTTCATGGATGTAAAATGCCAAGGTTGTTTCAATATAACCACTGTCTTTAGCCACTCTCAAACCGTTGTGGTGTGCGGTAACTGTCAGACCGTCTTGTGCCAGCCAACAGGTGGCCGTGCAAGGCTAACAGAAGGATGCTCCTTTAGGAAGAAGGGAGACTAA

Protein sequence

>Msa0235050

MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRKKGD*