AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1279910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1279910 MtrunA17_Chr8g0349761 90.426 94 9 0 16 109 22 115 7.07e-54 170
Msa1279910 MtrunA17_Chr8g0349771 89.247 93 10 0 16 108 21 113 2.20e-52 166
Msa1279910 MtrunA17_Chr8g0349851 84.444 90 14 0 16 105 21 110 1.09e-45 149
Msa1279910 MtrunA17_Chr8g0349811 81.720 93 16 1 16 107 21 113 4.27e-45 147
Msa1279910 MtrunA17_Chr8g0349801 83.529 85 14 0 16 100 21 105 1.97e-44 145
Msa1279910 MtrunA17_Chr8g0349831 80.645 93 17 1 16 107 22 114 1.08e-43 144
Msa1279910 MtrunA17_Chr8g0349751 79.348 92 19 0 16 107 71 162 2.82e-43 144
Msa1279910 MtrunA17_Chr8g0349781 79.570 93 18 1 16 107 21 113 1.64e-42 140
Msa1279910 MtrunA17_Chr8g0349701 60.227 88 35 0 17 104 22 109 1.27e-32 115
Msa1279910 MtrunA17_Chr8g0349721 63.095 84 31 0 17 100 22 105 1.45e-32 115
Msa1279910 MtrunA17_Chr7g0247011 63.636 77 28 0 16 92 21 97 2.00e-29 106
Msa1279910 MtrunA17_Chr8g0349641 61.628 86 33 0 17 102 22 107 5.08e-29 105
Msa1279910 MtrunA17_Chr5g0425391 62.069 87 33 0 17 103 22 108 6.20e-29 105
Msa1279910 MtrunA17_Chr8g0349671 60.465 86 34 0 17 102 22 107 1.45e-28 104
Msa1279910 MtrunA17_Chr7g0247041 63.095 84 30 1 17 99 21 104 4.66e-28 103
Msa1279910 MtrunA17_Chr8g0349741 55.435 92 38 2 16 106 21 110 4.96e-27 100
Msa1279910 MtrunA17_Chr7g0267881 56.250 80 30 1 16 90 23 102 5.06e-22 86.3
Msa1279910 MtrunA17_Chr3g0118511 50.000 88 43 1 16 103 17 103 4.95e-21 84.7
Msa1279910 MtrunA17_Chr8g0349651 54.667 75 34 0 28 102 12 86 1.64e-20 83.2
Msa1279910 MtrunA17_Chr7g0247031 53.846 78 35 1 16 92 22 99 7.00e-19 79.0
Msa1279910 MtrunA17_Chr3g0131521 42.683 82 45 1 16 97 49 128 3.44e-15 69.3
Msa1279910 MtrunA17_Chr1g0160511 40.789 76 43 1 16 91 54 127 1.27e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1279910 AT3G61220.1 62.791 86 29 3 16 99 21 105 2.54e-28 104
Msa1279910 AT3G61220.2 62.791 86 29 3 16 99 28 112 3.48e-28 104
Msa1279910 AT3G61220.3 62.791 86 29 3 16 99 52 136 6.03e-28 103
Msa1279910 AT2G24190.2 59.302 86 32 3 16 99 26 110 3.88e-26 99.0
Msa1279910 AT2G24190.1 59.302 86 32 3 16 99 21 105 3.98e-26 98.6
Msa1279910 AT1G01800.1 56.627 83 34 2 16 96 19 101 6.51e-24 92.8
Msa1279910 AT5G51030.1 37.634 93 54 2 16 106 52 142 1.58e-16 73.2
Msa1279910 AT5G61830.1 41.935 93 52 1 16 106 51 143 2.49e-15 70.1
Msa1279910 AT5G61830.2 41.935 93 52 1 16 106 51 143 2.49e-15 70.1

Find 17 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATATCAAGTTTGCCAAAT+TGG 0.158459 8_3:+17644525 None:intergenic
ATTTGGCAAACTTGATATTC+TGG 0.220906 8_3:-17644522 Msa1279910:intron
TGTAATTAATGACAAAGATT+TGG 0.327767 8_3:-17644158 Msa1279910:CDS
ATCTTTAGTTGAAATTGAAT+TGG 0.348256 8_3:+17645083 None:intergenic
AATATCAAGTTTGCCAAATT+GGG 0.350754 8_3:+17644526 None:intergenic
GAAAAGAGGTCTTCATGCTT+TGG 0.381657 8_3:-17644648 Msa1279910:CDS
AATTCAATTTCAACTAAAGA+TGG 0.404243 8_3:-17645081 Msa1279910:CDS
TTGGAAACACTCAAAGCATC+TGG 0.405378 8_3:-17644629 Msa1279910:CDS
GTTAACAATGCAGGGATTAG+TGG 0.432718 8_3:-17644184 Msa1279910:CDS
TGAATGCAGGTTAACAATGC+AGG 0.492400 8_3:-17644193 Msa1279910:intron
AGGGTTAGAGATAAGCAAAC+TGG 0.506423 8_3:+17644135 None:intergenic
GTTAAGCAGTTAGCTTCAGA+TGG 0.527204 8_3:-17644701 Msa1279910:CDS
ATTCAATTTCAACTAAAGAT+GGG 0.536049 8_3:-17645080 Msa1279910:CDS
ACAGCAAGAGATGAGAAAAG+AGG 0.563297 8_3:-17644662 Msa1279910:CDS
GAATGCAGGTTAACAATGCA+GGG 0.567392 8_3:-17644192 Msa1279910:intron
AGATGGGACAAACTGCAGAA+AGG 0.596749 8_3:-17645064 Msa1279910:intron
AGCTTCAGATGGAATCAAAG+TGG 0.667222 8_3:-17644690 Msa1279910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCATTTAAAAAAATGGAA+AGG + chr8_3:17644746-17644765 None:intergenic 15.0%
!! CAAAGTTTCATTTAAAAAAA+TGG + chr8_3:17644751-17644770 None:intergenic 15.0%
!!! ACTATTTTCATTTAAAACTA+AGG + chr8_3:17644808-17644827 None:intergenic 15.0%
!!! GTTTTACTAAATCTTTATTT+AGG - chr8_3:17644865-17644884 Msa1279910:intron 15.0%
!! TTTCATTTAAAAAAATGGAA+AGG + chr8_3:17644746-17644765 None:intergenic 15.0%
!! CAAAGTTTCATTTAAAAAAA+TGG + chr8_3:17644751-17644770 None:intergenic 15.0%
!!! ACTATTTTCATTTAAAACTA+AGG + chr8_3:17644808-17644827 None:intergenic 15.0%
!!! GTTTTACTAAATCTTTATTT+AGG - chr8_3:17644865-17644884 Msa1279910:intron 15.0%
!! ATCTTTAGTTGAAATTGAAT+TGG + chr8_3:17644142-17644161 None:intergenic 20.0%
!! AATTCAATTTCAACTAAAGA+TGG - chr8_3:17644141-17644160 Msa1279910:CDS 20.0%
!! ATTCAATTTCAACTAAAGAT+GGG - chr8_3:17644142-17644161 Msa1279910:CDS 20.0%
!!! AAAGTTTTAGAATTCATTCT+TGG - chr8_3:17644211-17644230 Msa1279910:intron 20.0%
!! ATTCTGGTAACATAAAAATT+TGG - chr8_3:17644716-17644735 Msa1279910:intron 20.0%
!! AAGGAATAAAAAAAGTAACT+TGG + chr8_3:17644789-17644808 None:intergenic 20.0%
!!! TTCTTGATAACTTAATTTGA+AGG - chr8_3:17644950-17644969 Msa1279910:intron 20.0%
!!! TCTTGATAACTTAATTTGAA+GGG - chr8_3:17644951-17644970 Msa1279910:intron 20.0%
!! TGTAATTAATGACAAAGATT+TGG - chr8_3:17645064-17645083 Msa1279910:intron 20.0%
!! ATCTTTAGTTGAAATTGAAT+TGG + chr8_3:17644142-17644161 None:intergenic 20.0%
!! AATTCAATTTCAACTAAAGA+TGG - chr8_3:17644141-17644160 Msa1279910:CDS 20.0%
!! ATTCAATTTCAACTAAAGAT+GGG - chr8_3:17644142-17644161 Msa1279910:CDS 20.0%
!!! AAAGTTTTAGAATTCATTCT+TGG - chr8_3:17644211-17644230 Msa1279910:intron 20.0%
!! ATTCTGGTAACATAAAAATT+TGG - chr8_3:17644716-17644735 Msa1279910:intron 20.0%
!! AAGGAATAAAAAAAGTAACT+TGG + chr8_3:17644789-17644808 None:intergenic 20.0%
!!! TTCTTGATAACTTAATTTGA+AGG - chr8_3:17644950-17644969 Msa1279910:intron 20.0%
!!! TCTTGATAACTTAATTTGAA+GGG - chr8_3:17644951-17644970 Msa1279910:intron 20.0%
!! TGTAATTAATGACAAAGATT+TGG - chr8_3:17645064-17645083 Msa1279910:intron 20.0%
! TTAGAATTCATTCTTGGTAA+TGG - chr8_3:17644217-17644236 Msa1279910:intron 25.0%
! AATATCAAGTTTGCCAAATT+GGG + chr8_3:17644699-17644718 None:intergenic 25.0%
! CATTTAAAAAAATGGAAAGG+AGG + chr8_3:17644743-17644762 None:intergenic 25.0%
!!! TATGAAAATTTGTTGAATGC+AGG - chr8_3:17645016-17645035 Msa1279910:intron 25.0%
! TTAGAATTCATTCTTGGTAA+TGG - chr8_3:17644217-17644236 Msa1279910:intron 25.0%
! AATATCAAGTTTGCCAAATT+GGG + chr8_3:17644699-17644718 None:intergenic 25.0%
! CATTTAAAAAAATGGAAAGG+AGG + chr8_3:17644743-17644762 None:intergenic 25.0%
!!! TATGAAAATTTGTTGAATGC+AGG - chr8_3:17645016-17645035 Msa1279910:intron 25.0%
! CATTTTGTCAAATCCCAATT+TGG - chr8_3:17644683-17644702 Msa1279910:CDS 30.0%
GAATATCAAGTTTGCCAAAT+TGG + chr8_3:17644700-17644719 None:intergenic 30.0%
ATTTGGCAAACTTGATATTC+TGG - chr8_3:17644700-17644719 Msa1279910:CDS 30.0%
TCATAACTCTTGTAACACAA+TGG + chr8_3:17644924-17644943 None:intergenic 30.0%
! CATTTTGTCAAATCCCAATT+TGG - chr8_3:17644683-17644702 Msa1279910:CDS 30.0%
GAATATCAAGTTTGCCAAAT+TGG + chr8_3:17644700-17644719 None:intergenic 30.0%
ATTTGGCAAACTTGATATTC+TGG - chr8_3:17644700-17644719 Msa1279910:CDS 30.0%
TCATAACTCTTGTAACACAA+TGG + chr8_3:17644924-17644943 None:intergenic 30.0%
!! GACAATATTCTAAAACCACG+CGG - chr8_3:17644435-17644454 Msa1279910:intron 35.0%
!! ACAATATTCTAAAACCACGC+GGG - chr8_3:17644436-17644455 Msa1279910:intron 35.0%
ACAGGATCAAACAAAGGAAT+TGG - chr8_3:17644488-17644507 Msa1279910:intron 35.0%
!! GACAATATTCTAAAACCACG+CGG - chr8_3:17644435-17644454 Msa1279910:intron 35.0%
!! ACAATATTCTAAAACCACGC+GGG - chr8_3:17644436-17644455 Msa1279910:intron 35.0%
ACAGGATCAAACAAAGGAAT+TGG - chr8_3:17644488-17644507 Msa1279910:intron 35.0%
! GTTAAGCAGTTAGCTTCAGA+TGG - chr8_3:17644521-17644540 Msa1279910:intron 40.0%
AGCTTCAGATGGAATCAAAG+TGG - chr8_3:17644532-17644551 Msa1279910:CDS 40.0%
ACAGCAAGAGATGAGAAAAG+AGG - chr8_3:17644560-17644579 Msa1279910:CDS 40.0%
GAAAAGAGGTCTTCATGCTT+TGG - chr8_3:17644574-17644593 Msa1279910:CDS 40.0%
! TTGGAAACACTCAAAGCATC+TGG - chr8_3:17644593-17644612 Msa1279910:CDS 40.0%
TGAATGCAGGTTAACAATGC+AGG - chr8_3:17645029-17645048 Msa1279910:intron 40.0%
GAATGCAGGTTAACAATGCA+GGG - chr8_3:17645030-17645049 Msa1279910:intron 40.0%
GTTAACAATGCAGGGATTAG+TGG - chr8_3:17645038-17645057 Msa1279910:intron 40.0%
AGGGTTAGAGATAAGCAAAC+TGG + chr8_3:17645090-17645109 None:intergenic 40.0%
! GTTAAGCAGTTAGCTTCAGA+TGG - chr8_3:17644521-17644540 Msa1279910:intron 40.0%
AGCTTCAGATGGAATCAAAG+TGG - chr8_3:17644532-17644551 Msa1279910:CDS 40.0%
ACAGCAAGAGATGAGAAAAG+AGG - chr8_3:17644560-17644579 Msa1279910:CDS 40.0%
GAAAAGAGGTCTTCATGCTT+TGG - chr8_3:17644574-17644593 Msa1279910:CDS 40.0%
! TTGGAAACACTCAAAGCATC+TGG - chr8_3:17644593-17644612 Msa1279910:CDS 40.0%
TGAATGCAGGTTAACAATGC+AGG - chr8_3:17645029-17645048 Msa1279910:intron 40.0%
GAATGCAGGTTAACAATGCA+GGG - chr8_3:17645030-17645049 Msa1279910:intron 40.0%
GTTAACAATGCAGGGATTAG+TGG - chr8_3:17645038-17645057 Msa1279910:intron 40.0%
AGGGTTAGAGATAAGCAAAC+TGG + chr8_3:17645090-17645109 None:intergenic 40.0%
AGATGGGACAAACTGCAGAA+AGG - chr8_3:17644158-17644177 Msa1279910:CDS 45.0%
GTTTGTTTCCAGGATTGCAG+TGG - chr8_3:17644463-17644482 Msa1279910:intron 45.0%
GTGGTGACAGGATCAAACAA+AGG - chr8_3:17644482-17644501 Msa1279910:intron 45.0%
AGATGGGACAAACTGCAGAA+AGG - chr8_3:17644158-17644177 Msa1279910:CDS 45.0%
GTTTGTTTCCAGGATTGCAG+TGG - chr8_3:17644463-17644482 Msa1279910:intron 45.0%
GTGGTGACAGGATCAAACAA+AGG - chr8_3:17644482-17644501 Msa1279910:intron 45.0%
TCCAGGATTGCAGTGGTGAC+AGG - chr8_3:17644470-17644489 Msa1279910:intron 55.0%
TCCTGTCACCACTGCAATCC+TGG + chr8_3:17644474-17644493 None:intergenic 55.0%
TCCAGGATTGCAGTGGTGAC+AGG - chr8_3:17644470-17644489 Msa1279910:intron 55.0%
TCCTGTCACCACTGCAATCC+TGG + chr8_3:17644474-17644493 None:intergenic 55.0%
GGAAACAAACACAGCCCGCG+TGG + chr8_3:17644453-17644472 None:intergenic 60.0%
! CGCGGGCTGTGTTTGTTTCC+AGG - chr8_3:17644453-17644472 Msa1279910:intron 60.0%
GGAAACAAACACAGCCCGCG+TGG + chr8_3:17644453-17644472 None:intergenic 60.0%
! CGCGGGCTGTGTTTGTTTCC+AGG - chr8_3:17644453-17644472 Msa1279910:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 17644132 17645112 17644132 ID=Msa1279910;Name=Msa1279910
chr8_3 mRNA 17644132 17645112 17644132 ID=Msa1279910-mRNA-1;Parent=Msa1279910;Name=Msa1279910-mRNA-1;_AED=0.68;_eAED=0.68;_QI=0|0|0|0.33|1|1|3|0|109
chr8_3 exon 17645065 17645112 17645065 ID=Msa1279910-mRNA-1:exon:6841;Parent=Msa1279910-mRNA-1
chr8_3 exon 17644523 17644726 17644523 ID=Msa1279910-mRNA-1:exon:6840;Parent=Msa1279910-mRNA-1
chr8_3 exon 17644132 17644206 17644132 ID=Msa1279910-mRNA-1:exon:6839;Parent=Msa1279910-mRNA-1
chr8_3 CDS 17645065 17645112 17645065 ID=Msa1279910-mRNA-1:cds;Parent=Msa1279910-mRNA-1
chr8_3 CDS 17644523 17644726 17644523 ID=Msa1279910-mRNA-1:cds;Parent=Msa1279910-mRNA-1
chr8_3 CDS 17644132 17644206 17644132 ID=Msa1279910-mRNA-1:cds;Parent=Msa1279910-mRNA-1
Gene Sequence

>Msa1279910

ATGCTCACCAATTCAATTTCAACTAAAGATGGGACAAACTGCAGAAAGATAGTTAAGCAGTTAGCTTCAGATGGAATCAAAGTGGTGCTTACAGCAAGAGATGAGAAAAGAGGTCTTCATGCTTTGGAAACACTCAAAGCATCTGGTTTATCTGATTTTGTTGTATTTCATCGACTAGACGTTGCTGATGCTGCAAGTGTAGCTACTGTTGCACATTTTGTCAAATCCCAATTTGGCAAACTTGATATTCTGGTTAACAATGCAGGGATTAGTGGAGCTGTAATTAATGACAAAGATTTGGCCAGTTTGCTTATCTCTAACCCTAGA

Protein sequence

>Msa1279910

MLTNSISTKDGTNCRKIVKQLASDGIKVVLTARDEKRGLHALETLKASGLSDFVVFHRLDVADAASVATVAHFVKSQFGKLDILVNNAGISGAVINDKDLASLLISNPR