AlfalfaGEDB | Msa1318550

M. sativa cultivar zhongmu-4 / Msa1318550

KEGG: K13667
GO: -
PFAM: PF05686.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1318550	sp\|B0X1Q4\|RUMI_CULQU	24.646	353	230	9	97	431	68	402	8.58e-25	109

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1318550	A0A072TN43	95.968	496	20	0	1	496	1	496	0.0	979

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0090380	Msa1318550	0.810228	1.279349e-50	-8.615850e-47
Msa0094710	Msa1318550	0.811148	8.094000e-51	-8.615850e-47
Msa0137860	Msa1318550	0.805484	1.305858e-49	-8.615850e-47
Msa0150620	Msa1318550	0.827522	1.493571e-54	-8.615850e-47
Msa0186240	Msa1318550	0.803857	2.854109e-49	-8.615850e-47
Msa0319680	Msa1318550	0.805931	1.051913e-49	-8.615850e-47
Msa0345050	Msa1318550	0.826119	3.230908e-54	-8.615850e-47
Msa0345890	Msa1318550	0.823004	1.748775e-53	-8.615850e-47
Msa0355550	Msa1318550	0.810300	1.234175e-50	-8.615850e-47
Msa0383900	Msa1318550	0.820354	7.170303e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1318550	MtrunA17_Chr8g0349241	95.968	496	20	0	1	496	1	496	0.0	979
Msa1318550	MtrunA17_Chr7g0247261	61.220	410	154	3	63	468	35	443	0.0	572
Msa1318550	MtrunA17_Chr8g0349361	54.244	483	208	7	12	488	26	501	0.0	559
Msa1318550	MtrunA17_Chr7g0247251	63.969	383	136	1	88	468	14	396	0.0	557
Msa1318550	MtrunA17_Chr8g0349371	57.870	432	177	5	60	488	55	484	0.0	534
Msa1318550	MtrunA17_Chr6g0471441	54.118	425	194	1	65	488	70	494	0.0	530
Msa1318550	MtrunA17_Chr5g0397701	51.434	488	196	9	25	488	21	491	1.57e-175	503
Msa1318550	MtrunA17_Chr3g0104841	43.434	396	210	6	70	464	81	463	3.12e-120	360

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1318550	AT5G23850.1	55.083	423	185	2	65	482	99	521	0.0	538
Msa1318550	AT2G45830.4	51.059	472	214	6	31	488	39	507	0.0	530
Msa1318550	AT2G45830.1	51.059	472	214	6	31	488	39	507	0.0	530
Msa1318550	AT3G61270.1	49.272	481	235	4	11	488	10	484	1.01e-178	511
Msa1318550	AT3G48980.2	51.259	437	203	4	62	488	94	530	1.45e-178	512
Msa1318550	AT3G48980.1	51.259	437	203	4	62	488	88	524	1.45e-178	512
Msa1318550	AT2G45830.3	56.284	366	160	0	123	488	1	366	2.54e-168	480
Msa1318550	AT2G45830.6	56.284	366	160	0	123	488	1	366	2.54e-168	480
Msa1318550	AT2G45830.2	56.284	366	160	0	123	488	1	366	2.54e-168	480
Msa1318550	AT2G45830.5	56.284	366	160	0	123	488	1	366	2.54e-168	480

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Find 86 sgRNAs with CRISPR-Local

Find 374 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTTCCAAGACACAAAAT+TGG	0.265181	8_4:-20634406	Msa1318550:CDS
AAGAAAAGGAGAATTTAATA+CGG	0.545091	8_4:-20633026	Msa1318550:CDS
AAGGAAACATTTCATGGTTG+AGG	0.463785	8_4:-20633130	Msa1318550:CDS
AAGGAACATGCCATACTTTC+AGG	0.348711	8_4:+20633162	None:intergenic
AAGGCAACAAGAATGTCAAA+TGG	0.510499	8_4:-20634665	Msa1318550:CDS
AAGGGATGTGTTCACAATAT+GGG	0.434287	8_4:-20635404	Msa1318550:CDS
AATACCTGAAGGAGCGGTTG+AGG	0.538729	8_4:-20633190	Msa1318550:CDS
AATACGGCAAGTGAAGACAA+GGG	0.753746	8_4:-20633010	Msa1318550:CDS
AATAGTAGTGCTTGTTACTT+TGG	0.349075	8_4:+20635729	None:intergenic
AATGTTGTTGGGTAGGAAGA+TGG	0.541971	8_4:+20635619	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATAGCATATATGACTTACA+AGG	+	chr8_4:20633121-20633140	None:intergenic	30.0%
TTTGGATAAGAGAACTTAAG+AGG	+	chr8_4:20633476-20633495	None:intergenic	30.0%
CTTAATACTGTGAGTATGTT+TGG	+	chr8_4:20633494-20633513	None:intergenic	30.0%
TATTTGGTATATGTGCACTA+AGG	-	chr8_4:20633621-20633640	Msa1318550:intron	30.0%
GAACTTGTATGACTTGAATT+TGG	-	chr8_4:20633668-20633687	Msa1318550:intron	30.0%
AACAATAACATGTAAGAGGT+TGG	-	chr8_4:20633860-20633879	Msa1318550:intron	30.0%
ACAATAACATGTAAGAGGTT+GGG	-	chr8_4:20633861-20633880	Msa1318550:intron	30.0%
TTGGGAATAAACCAAACTAT+TGG	-	chr8_4:20633879-20633898	Msa1318550:intron	30.0%
TAAACAAACATCATCTTACC+AGG	+	chr8_4:20634052-20634071	None:intergenic	30.0%
TGTATTCATTTCAAAGTCTC+AGG	-	chr8_4:20634083-20634102	Msa1318550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	20632990	20639519	20632990	ID=Msa1318550;Name=Msa1318550
chr8_4	mRNA	20632990	20639519	20632990	ID=Msa1318550-mRNA-1;Parent=Msa1318550;Name=Msa1318550-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|0.8\|0.83\|6\|0\|496
chr8_4	exon	20639388	20639519	20639388	ID=Msa1318550-mRNA-1:exon:7584;Parent=Msa1318550-mRNA-1
chr8_4	exon	20635208	20635752	20635208	ID=Msa1318550-mRNA-1:exon:7583;Parent=Msa1318550-mRNA-1
chr8_4	exon	20634545	20634737	20634545	ID=Msa1318550-mRNA-1:exon:7582;Parent=Msa1318550-mRNA-1
chr8_4	exon	20634387	20634451	20634387	ID=Msa1318550-mRNA-1:exon:7581;Parent=Msa1318550-mRNA-1
chr8_4	exon	20634041	20634251	20634041	ID=Msa1318550-mRNA-1:exon:7580;Parent=Msa1318550-mRNA-1
chr8_4	exon	20632990	20633334	20632990	ID=Msa1318550-mRNA-1:exon:7579;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20639388	20639519	20639388	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20635208	20635752	20635208	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20634545	20634737	20634545	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20634387	20634451	20634387	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20634041	20634251	20634041	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1
chr8_4	CDS	20632990	20633334	20632990	ID=Msa1318550-mRNA-1:cds;Parent=Msa1318550-mRNA-1

Gene Sequence

>Msa1318550

ATGGCCAATAGTCAAAGCTTTCATCAAGCATACAACAAAACAAAAAGATGGGTTTCAACTTCAGCCTTATTCTCCTTCTTCTTCTTGTTTTTATTTGCTATAAGCACTTTCATCATCCATTGGATTGACCTTTCCAAAGTAACAAGCACTACTATTTTCAAGACAATAGAAGTCTTCAACAACATTAAACAACAACCTCAATTCCCACTAAATTGCAACAATGAAACCTCAAGCTCAACTTGTCCATCTTCCTACCCAACAACATTTCATCTCAATGATGATTCACCATCCTCATCATGTCCAAATTACTTCAAATGGATCCATGAAGATCTTAAACCATGGGAGAATACAGGAATCACAAGAGAAATGGTTGAAAGAGGAAAAAACATGTCACATTTTAGGCTTGTGGTTGTGGATGGAAAAGCATATATAGATAAATATGCAAAATCTTATCAAACAAGGGATGTGTTCACAATATGGGGAATTTTACAACTTTTAAGGTTGTACCCTGGAAAAATACCTGATTTGGAGCTAATGTTTCAATGTGGTGATAAAAATGTGGTGGACAAGAAAAAATTTCAAGGGCCAGAAAATGTGTCACCTCCACCTATTTTTCATTATTGTGGAGATGAAGATACACTTGACATTGTTTTTCCTGATTGGACCTTCTGGGGTTGGGCTGAGCTCCACATAAGACCATGGGAAACAACATTACGCAATATACAAGAAGGCAACAAGAATGTCAAATGGAAGGATAGAATACCTTATGCATTTTGGAAGGGAAATCCAGCAGTATCTCCCATTAGAAAAGAACTCGAAAAGTGTAATGCATCCGAAGAACATGATTGGAATGCTCGAATATATGACATCCAATGGCAACAGGAGAGATCAAAAAATTTCCAAGACACAAAATTGGAAAATCAATGTACCTATAGGTACAAGATTTATGCAGAAGGGGCAACATGGTCTGTGAGTGAAAAGTACATAATAGCATGTGACTCCATGACTATGTTCATAGAGCCTAAGTACTATGATTTCTTTACAAGAAACTTGCTACCTTTGCAGCACTATTGGCCTATCAGTGCCAAAAACATGTGTGAAGAGATTAAGTTTGCTGTGGATTGGGGCAATGCTCACCTTGACAAAGCACAAAAAATTGGAGAAGGAGGAACCAACTACATCATAGATAATTTGAATATGAAGTTTGTTTATGATTACATGTTTCATCTATTAAATAGTTATGCTAAGCTTTTGAGATTCAAACCAAAAATACCTGAAGGAGCGGTTGAGGTTTGTCCTGAAAGTATGGCATGTTCCTTGCGTGGTGCAAGGAAACATTTCATGGTTGAGGGCATGGTGATGTCACCAAGTGATACACCTCCATGCACAATGCCTTCTCCTTATACAACTCAAACTCTTCATCAATTTCTACAAGAAAAGGAGAATTTAATACGGCAAGTGAAGACAAGGGCCATGAATAAAGAGTTATAG

Protein sequence

>Msa1318550

MANSQSFHQAYNKTKRWVSTSALFSFFFLFLFAISTFIIHWIDLSKVTSTTIFKTIEVFNNIKQQPQFPLNCNNETSSSTCPSSYPTTFHLNDDSPSSSCPNYFKWIHEDLKPWENTGITREMVERGKNMSHFRLVVVDGKAYIDKYAKSYQTRDVFTIWGILQLLRLYPGKIPDLELMFQCGDKNVVDKKKFQGPENVSPPPIFHYCGDEDTLDIVFPDWTFWGWAELHIRPWETTLRNIQEGNKNVKWKDRIPYAFWKGNPAVSPIRKELEKCNASEEHDWNARIYDIQWQQERSKNFQDTKLENQCTYRYKIYAEGATWSVSEKYIIACDSMTMFIEPKYYDFFTRNLLPLQHYWPISAKNMCEEIKFAVDWGNAHLDKAQKIGEGGTNYIIDNLNMKFVYDYMFHLLNSYAKLLRFKPKIPEGAVEVCPESMACSLRGARKHFMVEGMVMSPSDTPPCTMPSPYTTQTLHQFLQEKENLIRQVKTRAMNKEL*