Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401860 | AET00863.2 | 100.000 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 1.07e-35 | 124 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401860 | G7K4U3 | 100.000 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 5.13e-36 | 124 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001450 | Msa1401860 | 0.815762 | 7.827775e-52 | -8.615850e-47 |
| Msa0026130 | Msa1401860 | 0.822441 | 2.364555e-53 | -8.615850e-47 |
| Msa0069330 | Msa1401860 | 0.806959 | 6.383453e-50 | -8.615850e-47 |
| Msa0073670 | Msa1401860 | 0.826834 | 2.182574e-54 | -8.615850e-47 |
| Msa0082280 | Msa1401860 | 0.800574 | 1.352027e-48 | -8.615850e-47 |
| Msa0109050 | Msa1401860 | 0.802810 | 4.700516e-49 | -8.615850e-47 |
| Msa0112790 | Msa1401860 | 0.815506 | 8.928210e-52 | -8.615850e-47 |
| Msa1367590 | Msa1401860 | 0.835970 | 1.230151e-56 | -8.615850e-47 |
| Msa1401860 | Msa1404190 | 0.817004 | 4.127839e-52 | -8.615850e-47 |
| Msa0687630 | Msa1401860 | 0.831338 | 1.766853e-55 | -8.615850e-47 |
| Msa0692840 | Msa1401860 | 0.819027 | 1.440917e-52 | -8.615850e-47 |
| Msa0713820 | Msa1401860 | 0.816037 | 6.798394e-52 | -8.615850e-47 |
| Msa0719510 | Msa1401860 | 0.805364 | 1.383615e-49 | -8.615850e-47 |
| Msa0756060 | Msa1401860 | 0.800425 | 1.450100e-48 | -8.615850e-47 |
| Msa0797750 | Msa1401860 | 0.817168 | 3.793652e-52 | -8.615850e-47 |
| Msa0125450 | Msa1401860 | 0.804330 | 2.274604e-49 | -8.615850e-47 |
| Msa0125540 | Msa1401860 | 0.807958 | 3.920004e-50 | -8.615850e-47 |
| Msa0166590 | Msa1401860 | 0.823950 | 1.050829e-53 | -8.615850e-47 |
| Msa0181610 | Msa1401860 | 0.805621 | 1.221684e-49 | -8.615850e-47 |
| Msa1227690 | Msa1401860 | 0.810015 | 1.421733e-50 | -8.615850e-47 |
| Msa1251160 | Msa1401860 | 0.813205 | 2.880330e-51 | -8.615850e-47 |
| Msa1307950 | Msa1401860 | 0.822493 | 2.299658e-53 | -8.615850e-47 |
| Msa0369450 | Msa1401860 | 0.822056 | 2.905407e-53 | -8.615850e-47 |
| Msa0380300 | Msa1401860 | 0.802419 | 5.659860e-49 | -8.615850e-47 |
| Msa0405710 | Msa1401860 | 0.817849 | 2.663557e-52 | -8.615850e-47 |
| Msa1019260 | Msa1401860 | 0.813730 | 2.207665e-51 | -8.615850e-47 |
| Msa1126510 | Msa1401860 | 0.829082 | 6.280032e-55 | -8.615850e-47 |
| Msa1126770 | Msa1401860 | 0.814890 | 1.223502e-51 | -8.615850e-47 |
| Msa1166860 | Msa1401860 | 0.811089 | 8.336153e-51 | -8.615850e-47 |
| Msa1184370 | Msa1401860 | 0.806829 | 6.803510e-50 | -8.615850e-47 |
| Msa0524500 | Msa1401860 | 0.818274 | 2.134952e-52 | -8.615850e-47 |
| Msa0552810 | Msa1401860 | 0.831423 | 1.683179e-55 | -8.615850e-47 |
| Msa0581070 | Msa1401860 | 0.819046 | 1.426480e-52 | -8.615850e-47 |
| Msa0931460 | Msa1401860 | 0.805923 | 1.056032e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401860 | MtrunA17_Chr5g0447181 | 86.154 | 65 | 6 | 1 | 1 | 62 | 1 | 65 | 1.15e-32 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1401860 | AT3G13275.1 | 63.636 | 44 | 15 | 1 | 1 | 44 | 1 | 43 | 3.96e-14 | 60.5 |
Find 17 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCGGAGGAAGGCCGTTCT+TGG | 0.245467 | tig0022765:+97129 | Msa1401860:CDS |
| ATCGGAATTCTGTTTGGATT+TGG | 0.287774 | tig0022765:+97049 | Msa1401860:CDS |
| ATCACGATCGGAATTCTGTT+TGG | 0.387585 | tig0022765:+97043 | Msa1401860:CDS |
| GAACGGTGTCTTTGACTTTG+TGG | 0.441601 | tig0022765:-97090 | None:intergenic |
| GGCGGAGGAAGGCCGTTCTT+GGG | 0.459430 | tig0022765:+97130 | Msa1401860:CDS |
| GTTCATTACTCTGATTCTTA+CGG | 0.479388 | tig0022765:+97109 | Msa1401860:CDS |
| TACTCTGATTCTTACGGCGG+AGG | 0.514379 | tig0022765:+97115 | Msa1401860:CDS |
| CTGATTCTTACGGCGGAGGA+AGG | 0.537452 | tig0022765:+97119 | Msa1401860:CDS |
| TTAATCAAGATGTGTTGTCC+AGG | 0.568505 | tig0022765:+96980 | None:intergenic |
| ACAATACTGAACTAGAACAA+TGG | 0.587324 | tig0022765:-97166 | None:intergenic |
| ATTGTGGTTGTGATCACGAT+CGG | 0.620795 | tig0022765:+97031 | Msa1401860:CDS |
| GCTATGCATATGCTTGATTG+TGG | 0.622895 | tig0022765:+97015 | Msa1401860:CDS |
| CTGAACTAGAACAATGGTGG+TGG | 0.629807 | tig0022765:-97160 | None:intergenic |
| CATTACTCTGATTCTTACGG+CGG | 0.653791 | tig0022765:+97112 | Msa1401860:CDS |
| GGTGGCGCATAACCCAAGAA+CGG | 0.667489 | tig0022765:-97142 | None:intergenic |
| ATACTGAACTAGAACAATGG+TGG | 0.667708 | tig0022765:-97163 | None:intergenic |
| GTAAGAATCAGAGTAATGAA+CGG | 0.707351 | tig0022765:-97107 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTAAGAATCAGAGTAATGAA+CGG | - | tig0022765:97110-97129 | None:intergenic | 30.0% | |
| GTTCATTACTCTGATTCTTA+CGG | + | tig0022765:97109-97128 | Msa1401860:CDS | 30.0% | |
| ATCGGAATTCTGTTTGGATT+TGG | + | tig0022765:97049-97068 | Msa1401860:CDS | 35.0% | |
| !! | GGATTTGGAGTTTTCAAGAA+TGG | + | tig0022765:97064-97083 | Msa1401860:CDS | 35.0% |
| GCTATGCATATGCTTGATTG+TGG | + | tig0022765:97015-97034 | Msa1401860:CDS | 40.0% | |
| ATTGTGGTTGTGATCACGAT+CGG | + | tig0022765:97031-97050 | Msa1401860:CDS | 40.0% | |
| ATCACGATCGGAATTCTGTT+TGG | + | tig0022765:97043-97062 | Msa1401860:CDS | 40.0% | |
| CATTACTCTGATTCTTACGG+CGG | + | tig0022765:97112-97131 | Msa1401860:CDS | 40.0% | |
| ! | GAACGGTGTCTTTGACTTTG+TGG | - | tig0022765:97093-97112 | None:intergenic | 45.0% |
| ! | CATAGCGTGCAGATTTTGCC+TGG | - | tig0022765:97001-97020 | None:intergenic | 50.0% |
| TACTCTGATTCTTACGGCGG+AGG | + | tig0022765:97115-97134 | Msa1401860:CDS | 50.0% | |
| CTGATTCTTACGGCGGAGGA+AGG | + | tig0022765:97119-97138 | Msa1401860:CDS | 55.0% | |
| GGTGGCGCATAACCCAAGAA+CGG | - | tig0022765:97145-97164 | None:intergenic | 55.0% | |
| GGCGGAGGAAGGCCGTTCTT+GGG | + | tig0022765:97130-97149 | Msa1401860:CDS | 65.0% | |
| CGGCGGAGGAAGGCCGTTCT+TGG | + | tig0022765:97129-97148 | Msa1401860:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022765 | gene | 96989 | 97177 | 96989 | ID=Msa1401860;Name=Msa1401860 |
| tig0022765 | mRNA | 96989 | 97177 | 96989 | ID=Msa1401860-mRNA-1;Parent=Msa1401860;Name=Msa1401860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|62 |
| tig0022765 | exon | 96989 | 97177 | 96989 | ID=Msa1401860-mRNA-1:exon:18608;Parent=Msa1401860-mRNA-1 |
| tig0022765 | CDS | 96989 | 97177 | 96989 | ID=Msa1401860-mRNA-1:cds;Parent=Msa1401860-mRNA-1 |
| Gene Sequence |
| Protein sequence |
