AlfalfaGEDB | Msa1424620

M. sativa cultivar zhongmu-4 / Msa1424620

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009506 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0030054 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771
PFAM: PF00403.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424620	sp\|Q9SZN7\|HIP26_ARATH	76.282	156	34	1	1	156	1	153	1.09e-83	245

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424620	G7J8I5	98.718	156	2	0	1	156	1	156	1.29e-112	325

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0118540	Msa1424620	0.801714	7.905351e-49	-8.615850e-47
Msa0186310	Msa1424620	0.830307	3.161303e-55	-8.615850e-47
Msa0189300	Msa1424620	0.810805	9.600561e-51	-8.615850e-47
Msa0231660	Msa1424620	0.823381	1.428170e-53	-8.615850e-47
Msa0251230	Msa1424620	0.809848	1.545078e-50	-8.615850e-47
Msa0271360	Msa1424620	0.814220	1.721313e-51	-8.615850e-47
Msa0335820	Msa1424620	-0.806079	9.790998e-50	-8.615850e-47
Msa0386040	Msa1424620	0.842944	1.896978e-58	-8.615850e-47
Msa0387910	Msa1424620	0.825189	5.369508e-54	-8.615850e-47
Msa0421260	Msa1424620	0.807559	4.765011e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424620	MtrunA17_Chr3g0145621	98.718	156	2	0	1	156	1	156	2.48e-116	325
Msa1424620	MtrunA17_Chr4g0036931	79.245	159	27	3	1	156	1	156	1.70e-88	254
Msa1424620	MtrunA17_Chr5g0405811	61.491	161	53	3	1	156	1	157	7.73e-66	197
Msa1424620	MtrunA17_Chr1g0160201	53.205	156	64	3	1	156	1	147	1.24e-53	166
Msa1424620	MtrunA17_Chr8g0382721	51.613	155	69	3	2	156	5	153	4.19e-49	155
Msa1424620	MtrunA17_Chr3g0124411	51.923	156	66	4	2	156	5	152	4.89e-49	155
Msa1424620	MtrunA17_Chr5g0407821	47.436	156	71	3	1	156	1	145	1.22e-45	146
Msa1424620	MtrunA17_Chr6g0448861	45.062	162	76	3	1	156	1	155	5.20e-45	145
Msa1424620	MtrunA17_Chr7g0251491	48.077	156	71	3	1	156	25	170	6.43e-45	145
Msa1424620	MtrunA17_Chr3g0115421	47.742	155	71	3	2	156	5	149	2.20e-42	138

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424620	AT4G38580.1	76.282	156	34	1	1	156	1	153	1.11e-84	245
Msa1424620	AT4G38580.2	76.282	156	34	1	1	156	1	153	1.11e-84	245
Msa1424620	AT4G35060.1	70.701	157	41	3	1	156	1	153	1.89e-76	224
Msa1424620	AT5G66110.3	66.667	138	44	1	19	156	12	147	2.19e-66	199
Msa1424620	AT5G66110.1	66.667	138	44	1	19	156	12	147	2.19e-66	199
Msa1424620	AT5G66110.2	65.854	123	40	1	34	156	1	121	1.48e-58	178
Msa1424620	AT1G71050.1	56.329	158	61	4	1	156	1	152	9.65e-54	167
Msa1424620	AT1G22990.1	49.057	159	71	2	1	156	1	152	2.00e-50	159
Msa1424620	AT1G22990.2	46.795	156	74	2	1	156	1	147	2.35e-47	150
Msa1424620	AT4G39700.1	48.734	158	74	4	2	156	5	158	1.24e-44	144

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Find 61 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGAAGATGGACTGCGA+AGG	0.769260	tig0025770:+68808	Msa1424620:CDS
AAAGTGAGAAGGTCGGTGGA+AGG	0.544215	tig0025770:+68841	Msa1424620:CDS
AAATCAAGTATTATGCCTTT+TGG	0.248220	tig0025770:+69268	Msa1424620:three_prime_UTR
AAATTGTTTGCGGTGTTTGT+GGG	0.476948	tig0025770:-68648	None:intergenic
AACATAGATAAATTTCCAAA+AGG	0.506301	tig0025770:-69283	None:intergenic
AAGAGATAATATGGGTGCTC+TGG	0.436844	tig0025770:+68586	Msa1424620:exon
AAGTAAAGAAAGAGATAATA+TGG	0.220139	tig0025770:+68577	Msa1424620:exon
AATAATTTGCTCTTCTTCTT+CGG	0.323900	tig0025770:-69441	None:intergenic
AATTTCATATAATGATTTGT+GGG	0.444929	tig0025770:+69358	Msa1424620:three_prime_UTR
ACGACGTCGTATGGAACATA+TGG	0.471597	tig0025770:-68991	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATTACCTGAAATTGTTTG+CGG	-	tig0025770:68661-68680	None:intergenic	30.0%
TGAATATGAATGGAATGGAA+TGG	-	tig0025770:68686-68705	None:intergenic	30.0%
ATATATATATGTACAGACGG+TGG	+	tig0025770:68780-68799	Msa1424620:intron	30.0%
CTTTCCTATCAATATCAACT+TGG	-	tig0025770:68882-68901	None:intergenic	30.0%
TCTTCGGAAAATCAATGATA+AGG	-	tig0025770:69428-69447	None:intergenic	30.0%
CTATTACCTGAAATTGTTTG+CGG	-	tig0025770:68661-68680	None:intergenic	30.0%
TGAATATGAATGGAATGGAA+TGG	-	tig0025770:68686-68705	None:intergenic	30.0%
ATATATATATGTACAGACGG+TGG	+	tig0025770:68780-68799	Msa1424620:intron	30.0%
CTTTCCTATCAATATCAACT+TGG	-	tig0025770:68882-68901	None:intergenic	30.0%
TCTTCGGAAAATCAATGATA+AGG	-	tig0025770:69428-69447	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025770	gene	68509	69471	68509	ID=Msa1424620;Name=Msa1424620
tig0025770	mRNA	68509	69471	68509	ID=Msa1424620-mRNA-1;Parent=Msa1424620;Name=Msa1424620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87\|1\|1\|1\|1\|1\|2\|283\|156
tig0025770	exon	68509	68673	68509	ID=Msa1424620-mRNA-1:exon:3833;Parent=Msa1424620-mRNA-1
tig0025770	exon	68796	69471	68796	ID=Msa1424620-mRNA-1:exon:3834;Parent=Msa1424620-mRNA-1
tig0025770	five_prime_UTR	68509	68595	68509	ID=Msa1424620-mRNA-1:five_prime_utr;Parent=Msa1424620-mRNA-1
tig0025770	CDS	68596	68673	68596	ID=Msa1424620-mRNA-1:cds;Parent=Msa1424620-mRNA-1
tig0025770	CDS	68796	69188	68796	ID=Msa1424620-mRNA-1:cds;Parent=Msa1424620-mRNA-1
tig0025770	three_prime_UTR	69189	69471	69189	ID=Msa1424620-mRNA-1:three_prime_utr;Parent=Msa1424620-mRNA-1

Gene Sequence

>Msa1424620

ATGGGTGCTCTGGATCACATTTCTGAGCTATTTGACTGCTCCGGTGGAAGTTCCCACAAACACCGCAAACAATTTCAGACGGTGGAAGTGAAAGTGAAGATGGACTGCGAAGGATGCGAGAGAAAAGTGAGAAGGTCGGTGGAAGGAATGAAAGGTGTGAACCAAGTTGATATTGATAGGAAAGCACACAAGGTCACTGTTCAGGGGTATGTTGAACCTAACAAAGTGGTGGCGCGTATCGCTCACCGCACTGGTAAGAGGGCTGAGATATGGCCATATGTTCCATACGACGTCGTTGCACACCCATATGCACAAGGTACGTACGACAAGAAAGCACCTTCCGGTTATGTTAGAAACAACTACGACAACAATCAGTATTCTGGATCCCATCTGGCGCGTGCTAGTTCCACCGAAGTTCGCTACACCACCGCTTTCAGCGACGAAAATCCCACCGCCTGTTCTGTCATGTGA

Protein sequence

>Msa1424620

MGALDHISELFDCSGGSSHKHRKQFQTVEVKVKMDCEGCERKVRRSVEGMKGVNQVDIDRKAHKVTVQGYVEPNKVVARIAHRTGKRAEIWPYVPYDVVAHPYAQGTYDKKAPSGYVRNNYDNNQYSGSHLARASSTEVRYTTAFSDENPTACSVM*