AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005125

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005125.t1 MTR_1g080770 100.000 126 0 0 1 126 1 126 6.93e-88 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005125.t1 AT1G08780 71.318 129 34 1 1 126 1 129 1.64e-62 187

Find 33 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTCTGTATGGGAAGTT+TGG 0.251421 1.1:-60049507 MS.gene005125:CDS
ACAGGAATCAAATGATAATT+TGG 0.284647 1.1:-60049721 MS.gene005125:intron
AGAAGCGACAAGCCAGAAAT+TGG 0.310931 1.1:-60049583 MS.gene005125:CDS
AGGTCGTTCGGTTTCAAATA+GGG 0.342219 1.1:-60049662 MS.gene005125:CDS
GAGGTCGTTCGGTTTCAAAT+AGG 0.358066 1.1:-60049663 MS.gene005125:CDS
CTCCCAGGTAACTTCCGTTT+CGG 0.367751 1.1:+60052532 None:intergenic
CGAATTTGTTGATGTTCTGC+TGG 0.380247 1.1:+60052507 None:intergenic
GATCCGAAACGGAAGTTACC+TGG 0.424382 1.1:-60052535 MS.gene005125:CDS
CAGCAGAACATCAACAAATT+CGG 0.438286 1.1:-60052506 MS.gene005125:CDS
GAACTGAAGAAAATTCTGTA+TGG 0.443165 1.1:-60049516 MS.gene005125:CDS
CTGCTCTCTACTTCATCCTT+AGG 0.477281 1.1:+60049621 None:intergenic
TCACAGATGAAGAGGTCGTT+CGG 0.479003 1.1:-60049674 MS.gene005125:CDS
TTGAATCAACAAGATGCAAC+AGG 0.487875 1.1:-60052645 None:intergenic
GATGTTCTGCTGGTCCTCCC+AGG 0.504132 1.1:+60052517 None:intergenic
GGTATTTGCTCATGTGCCTA+AGG 0.537164 1.1:-60049637 MS.gene005125:CDS
AACTGAAGAAAATTCTGTAT+GGG 0.540625 1.1:-60049515 MS.gene005125:CDS
TTGTGATGCGTAAAGGGAGG+AGG 0.548135 1.1:-60052557 MS.gene005125:intron
GGTCGTTCGGTTTCAAATAG+GGG 0.554503 1.1:-60049661 MS.gene005125:CDS
GGAATCAAATGATAATTTGG+AGG 0.556672 1.1:-60049718 MS.gene005125:CDS
TGGAGAATCTATCAATCTTG+AGG 0.571653 1.1:-60049487 MS.gene005125:CDS
CTAAGGATGAAGTAGAGAGC+AGG 0.573213 1.1:-60049620 MS.gene005125:CDS
GTTAATTCTCACAGATGAAG+AGG 0.578655 1.1:-60049682 MS.gene005125:CDS
TGATGAGATCAAAATCGCTA+AGG 0.599557 1.1:-60052453 MS.gene005125:intron
CCAGAAATTGGAAAAGCTTG+AGG 0.599943 1.1:-60049571 MS.gene005125:CDS
ATCCGAAACGGAAGTTACCT+GGG 0.600013 1.1:-60052534 MS.gene005125:CDS
CGTTCGGTTTCAAATAGGGG+AGG 0.636159 1.1:-60049658 MS.gene005125:CDS
AGAATCTATCAATCTTGAGG+AGG 0.639907 1.1:-60049484 MS.gene005125:CDS
AAAGGGAGGAGGATCCGAAA+CGG 0.642912 1.1:-60052546 MS.gene005125:intron
AGAACATCAACAAATTCGGA+AGG 0.648692 1.1:-60052502 MS.gene005125:CDS
TTCAGACATCTGAGCAACCA+CGG 0.661375 1.1:+60049536 None:intergenic
GAAATTGGAAAAGCTTGAGG+AGG 0.664223 1.1:-60049568 MS.gene005125:CDS
TGAGGAGGAGAAACAATCCG+TGG 0.717310 1.1:-60049553 MS.gene005125:CDS
CGAAACGGAAGTTACCTGGG+AGG 0.771650 1.1:-60052531 MS.gene005125:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAAATTAAAAAATAT+AGG - chr1.1:60051560-60051579 MS.gene005125:intron 0.0%
!! TTATTAAAATATGGTATAAA+CGG - chr1.1:60051680-60051699 MS.gene005125:intron 10.0%
!!! TTAATAACTTTTTTCAATTA+AGG + chr1.1:60050042-60050061 None:intergenic 10.0%
!! AAAAAAAATATATCAGCAAT+AGG - chr1.1:60051248-60051267 MS.gene005125:intron 15.0%
!! AAAAAAATATATCAGCAATA+GGG - chr1.1:60051249-60051268 MS.gene005125:intron 15.0%
!! AAAAACAAAAATACAAAATC+TGG - chr1.1:60050384-60050403 MS.gene005125:intron 15.0%
!! AAATAATATTTAGTTGTTTG+AGG - chr1.1:60052250-60052269 MS.gene005125:intron 15.0%
!! AACATCAAAATATAAAACAA+TGG + chr1.1:60052347-60052366 None:intergenic 15.0%
!! AATCTCAAAAATTCAATTTA+GGG + chr1.1:60049502-60049521 None:intergenic 15.0%
!! AATTATGGCTTATTAAAATA+TGG - chr1.1:60051671-60051690 MS.gene005125:intron 15.0%
!! ATATATATAATTCTTGTGAA+TGG + chr1.1:60051110-60051129 None:intergenic 15.0%
!!! ATACATGTCTTTTTTTTTTA+AGG + chr1.1:60051536-60051555 None:intergenic 15.0%
!!! ATTTTAAAGGAAAACAAAAA+TGG - chr1.1:60050248-60050267 MS.gene005125:intron 15.0%
!!! GTAAATTATTTTGAGAAATA+TGG - chr1.1:60051365-60051384 MS.gene005125:intron 15.0%
!!! TTTTCAATTTATTTCCATAA+AGG - chr1.1:60050767-60050786 MS.gene005125:intron 15.0%
!! AACAACTATAAAAATTTACC+TGG - chr1.1:60051311-60051330 MS.gene005125:intron 20.0%
!! CAATCTCAAAAATTCAATTT+AGG + chr1.1:60049503-60049522 None:intergenic 20.0%
!! CTCAAATAATGATAATTATC+TGG + chr1.1:60050671-60050690 None:intergenic 20.0%
!! TACAACCAAATTTATTAGAA+AGG + chr1.1:60051229-60051248 None:intergenic 20.0%
!! TATGGAAATAAATTGAAAAC+AGG + chr1.1:60050766-60050785 None:intergenic 20.0%
!!! ATAAGCACTTGTTATATAAA+AGG + chr1.1:60051897-60051916 None:intergenic 20.0%
!!! CTCAACATACATTTTTATTT+TGG - chr1.1:60050418-60050437 MS.gene005125:intron 20.0%
!!! GAGTGTTTCATTTATTTTAA+AGG - chr1.1:60050235-60050254 MS.gene005125:intron 20.0%
!!! TATATTTTGATGTTAATGCA+TGG - chr1.1:60052353-60052372 MS.gene005125:intron 20.0%
!!! TTAATCAAATTCAAAGGTTT+TGG - chr1.1:60049716-60049735 MS.gene005125:CDS 20.0%
!!! TTTGTTTTTTCCATTCAAAT+AGG + chr1.1:60050373-60050392 None:intergenic 20.0%
! AAACTATAAGAACGATATAG+CGG + chr1.1:60050640-60050659 None:intergenic 25.0%
! AACTGAAGAAAATTCTGTAT+GGG - chr1.1:60052596-60052615 MS.gene005125:intron 25.0%
! AATTTATAAGCTCAGATATG+TGG + chr1.1:60049840-60049859 None:intergenic 25.0%
! ACAATACAAATACAAATACC+AGG + chr1.1:60051332-60051351 None:intergenic 25.0%
! ACAGGAATCAAATGATAATT+TGG - chr1.1:60052390-60052409 MS.gene005125:intron 25.0%
! AGGATATAAACTACTCATAT+AGG + chr1.1:60049773-60049792 None:intergenic 25.0%
! CGTGTGTTAATTAAAGAATA+AGG - chr1.1:60051478-60051497 MS.gene005125:intron 25.0%
! CTATAAGCTATTTATCCAAA+CGG + chr1.1:60050795-60050814 None:intergenic 25.0%
! GGATATAAACTACTCATATA+GGG + chr1.1:60049772-60049791 None:intergenic 25.0%
! GTAGTGTTTGATAAAATTAG+CGG + chr1.1:60050079-60050098 None:intergenic 25.0%
! TAAAGGAAAACAAAAATGGT+TGG - chr1.1:60050252-60050271 MS.gene005125:intron 25.0%
! TAAAGTTGTTTATCCAAACA+AGG - chr1.1:60051932-60051951 MS.gene005125:intron 25.0%
! TATAAGCTATTTATCCAAAC+GGG + chr1.1:60050794-60050813 None:intergenic 25.0%
! TCTCACCTTTCTAATAAATT+TGG - chr1.1:60051221-60051240 MS.gene005125:intron 25.0%
! TTTGCGTTAATCAAATTCAA+AGG - chr1.1:60049710-60049729 MS.gene005125:CDS 25.0%
!! AAGAATAAGGTATTCTAGTT+AGG - chr1.1:60051491-60051510 MS.gene005125:intron 25.0%
!! CTTACATAATTTGAAGTGTT+TGG - chr1.1:60050912-60050931 MS.gene005125:intron 25.0%
!! TATCGTTCTTATAGTTTTTG+AGG - chr1.1:60050643-60050662 MS.gene005125:intron 25.0%
!!! AAAGGTTTTGGAATAGAAAT+GGG - chr1.1:60049728-60049747 MS.gene005125:intron 25.0%
!!! ATATAGGCTTTGAAGAAAAA+AGG - chr1.1:60051576-60051595 MS.gene005125:intron 25.0%
!!! TTTGTTTTCATAAGCTATCA+TGG + chr1.1:60051826-60051845 None:intergenic 25.0%
!!! TTTTTCAATTAAGGCTACAA+TGG + chr1.1:60050033-60050052 None:intergenic 25.0%
AAAATTCTGTATGGGAAGTT+TGG - chr1.1:60052604-60052623 MS.gene005125:intron 30.0%
AGAAAGTAGCTTATAGCTTA+AGG + chr1.1:60049887-60049906 None:intergenic 30.0%
ATAAGCTATTTATCCAAACG+GGG + chr1.1:60050793-60050812 None:intergenic 30.0%
ATCCTCGAAAATCAATTATG+TGG - chr1.1:60052109-60052128 MS.gene005125:intron 30.0%
ATGTGGTAAGCTCAAAATTA+TGG - chr1.1:60051656-60051675 MS.gene005125:intron 30.0%
ATTCAAATAGGAAACCTTCT+CGG + chr1.1:60050361-60050380 None:intergenic 30.0%
ATTTGTTTGTGATGCGTAAA+GGG - chr1.1:60049548-60049567 MS.gene005125:CDS 30.0%
CATTATTTGAGCTTAACTAC+TGG - chr1.1:60050680-60050699 MS.gene005125:intron 30.0%
CTCGATAAGCTATTTATGTT+TGG - chr1.1:60050870-60050889 MS.gene005125:intron 30.0%
GAACTGAAGAAAATTCTGTA+TGG - chr1.1:60052595-60052614 MS.gene005125:intron 30.0%
GGAATCAAATGATAATTTGG+AGG - chr1.1:60052393-60052412 MS.gene005125:intron 30.0%
GGATTCATATTGTATGATTC+TGG + chr1.1:60050340-60050359 None:intergenic 30.0%
TCAAAATAAGCCAATCCAAA+CGG - chr1.1:60051789-60051808 MS.gene005125:intron 30.0%
TCGGAAGAAAAAGAAATTAG+CGG - chr1.1:60050605-60050624 MS.gene005125:intron 30.0%
TGATGTATACATGTATGTGT+GGG + chr1.1:60050196-60050215 None:intergenic 30.0%
!! AACCACATAATTGATTTTCG+AGG + chr1.1:60052114-60052133 None:intergenic 30.0%
!! ATAGGGATTCTCTTTGATTT+TGG + chr1.1:60049755-60049774 None:intergenic 30.0%
!! TTATTAGAGAAAGCGTTCAT+CGG - chr1.1:60050586-60050605 MS.gene005125:intron 30.0%
!! TTTATTTTCTGCTTGAGTCA+AGG + chr1.1:60051965-60051984 None:intergenic 30.0%
!! TTTTTACAGCTTTTTAGTCG+TGG + chr1.1:60051005-60051024 None:intergenic 30.0%
!!! CAAAGGTTTTGGAATAGAAA+TGG - chr1.1:60049727-60049746 MS.gene005125:intron 30.0%
AAAATAAGCCAATCCAAACG+GGG - chr1.1:60051791-60051810 MS.gene005125:intron 35.0%
AAATAGCGAATCGCTGATAA+CGG + chr1.1:60050127-60050146 None:intergenic 35.0%
AAGTAGCTTATAGCTTAAGG+TGG + chr1.1:60049884-60049903 None:intergenic 35.0%
ACTCTGTCTTTGTTCATATG+AGG + chr1.1:60050219-60050238 None:intergenic 35.0%
AGAACATCAACAAATTCGGA+AGG - chr1.1:60049609-60049628 MS.gene005125:CDS 35.0%
AGAATCTATCAATCTTGAGG+AGG - chr1.1:60052627-60052646 MS.gene005125:intron 35.0%
AGGGGGTCATATATATATAC+TGG - chr1.1:60051614-60051633 MS.gene005125:intron 35.0%
ATAAGCTATCATGGAGAGTT+TGG + chr1.1:60051817-60051836 None:intergenic 35.0%
ATTGCAAAATGCTTAGAAGG+AGG + chr1.1:60052317-60052336 None:intergenic 35.0%
CAAAATAAGCCAATCCAAAC+GGG - chr1.1:60051790-60051809 MS.gene005125:intron 35.0%
CAGCAGAACATCAACAAATT+CGG - chr1.1:60049605-60049624 MS.gene005125:CDS 35.0%
CATACAATATGAATCCGAGA+AGG - chr1.1:60050344-60050363 MS.gene005125:intron 35.0%
CATTTGTTTGTGATGCGTAA+AGG - chr1.1:60049547-60049566 MS.gene005125:CDS 35.0%
GAGAAGGTTTCCTATTTGAA+TGG - chr1.1:60050360-60050379 MS.gene005125:intron 35.0%
GTCATTGCAAAATGCTTAGA+AGG + chr1.1:60052320-60052339 None:intergenic 35.0%
GTGATGTATACATGTATGTG+TGG + chr1.1:60050197-60050216 None:intergenic 35.0%
GTGGGAATAAGAATTGTTTG+TGG + chr1.1:60050178-60050197 None:intergenic 35.0%
GTTAATTCTCACAGATGAAG+AGG - chr1.1:60052429-60052448 MS.gene005125:intron 35.0%
TGATGAGATCAAAATCGCTA+AGG - chr1.1:60049658-60049677 MS.gene005125:CDS 35.0%
TGGAGAATCTATCAATCTTG+AGG - chr1.1:60052624-60052643 MS.gene005125:intron 35.0%
TGTGATGTCTTTCAACTTCT+CGG - chr1.1:60052220-60052239 MS.gene005125:intron 35.0%
TTACCCAAACGCATTTGAAA+AGG - chr1.1:60051735-60051754 MS.gene005125:intron 35.0%
TTGAGATAGTGCTATCTGTT+TGG - chr1.1:60049810-60049829 MS.gene005125:intron 35.0%
TTGATCAGTTCTATAGTGCT+AGG + chr1.1:60050466-60050485 None:intergenic 35.0%
TTGCAAAATGCTTAGAAGGA+GGG + chr1.1:60052316-60052335 None:intergenic 35.0%
! AAGCCTTTTCAAATGCGTTT+GGG + chr1.1:60051741-60051760 None:intergenic 35.0%
! CAACAAAAAGCATGTAGCAA+AGG + chr1.1:60049793-60049812 None:intergenic 35.0%
! TAAGCCTTTTCAAATGCGTT+TGG + chr1.1:60051742-60051761 None:intergenic 35.0%
!! ATGGAGATTTTGTTTGTCAC+AGG - chr1.1:60052372-60052391 MS.gene005125:intron 35.0%
!! TTCTGGTTCCTATTTCCTAT+CGG + chr1.1:60050323-60050342 None:intergenic 35.0%
ACACGACACACATGAGAAAA+GGG + chr1.1:60052147-60052166 None:intergenic 40.0%
AGAAACTGTACGTCTCTGTT+TGG + chr1.1:60050154-60050173 None:intergenic 40.0%
AGCTTACCACATAGCTTATG+CGG + chr1.1:60051648-60051667 None:intergenic 40.0%
AGGTCGTTCGGTTTCAAATA+GGG - chr1.1:60052449-60052468 MS.gene005125:intron 40.0%
ATTTCCTATCGGGATGATTC+TGG + chr1.1:60050312-60050331 None:intergenic 40.0%
CCAGAAATTGGAAAAGCTTG+AGG - chr1.1:60052540-60052559 MS.gene005125:CDS 40.0%
CTTTGCTAGGTTTGAGGAAA+AGG - chr1.1:60052045-60052064 MS.gene005125:intron 40.0%
GAAATTGGAAAAGCTTGAGG+AGG - chr1.1:60052543-60052562 MS.gene005125:CDS 40.0%
GAATCATCCCGATAGGAAAT+AGG - chr1.1:60050312-60050331 MS.gene005125:intron 40.0%
TAATCTCCGCATAAGCTATG+TGG - chr1.1:60051639-60051658 MS.gene005125:intron 40.0%
TGCAAAATGCTTAGAAGGAG+GGG + chr1.1:60052315-60052334 None:intergenic 40.0%
TGCTAGGAGTTCAAAGTAAG+AGG + chr1.1:60050450-60050469 None:intergenic 40.0%
TGTCTTTCAACTTCTCGGTT+TGG - chr1.1:60052225-60052244 MS.gene005125:intron 40.0%
TTTGAGGAAAAGGATGAGAG+TGG - chr1.1:60052055-60052074 MS.gene005125:intron 40.0%
! CCTCAAGCTTTTCCAATTTC+TGG + chr1.1:60052543-60052562 None:intergenic 40.0%
! TCTGGTTCCTATTTCCTATC+GGG + chr1.1:60050322-60050341 None:intergenic 40.0%
!! AAAGGTAAAGCGAGTATCGA+AGG - chr1.1:60051594-60051613 MS.gene005125:intron 40.0%
!! AAGGTAAAGCGAGTATCGAA+GGG - chr1.1:60051595-60051614 MS.gene005125:intron 40.0%
!! CGAATTTGTTGATGTTCTGC+TGG + chr1.1:60049607-60049626 None:intergenic 40.0%
AAGCGCTTGAGAGACTGTTT+GGG - chr1.1:60050706-60050725 MS.gene005125:intron 45.0%
ACAACCAGAATCATCCCGAT+AGG - chr1.1:60050305-60050324 MS.gene005125:intron 45.0%
AGAAGCGACAAGCCAGAAAT+TGG - chr1.1:60052528-60052547 MS.gene005125:CDS 45.0%
ATCCGAAACGGAAGTTACCT+GGG - chr1.1:60049577-60049596 MS.gene005125:CDS 45.0%
ATTTCCATAAAGGCCCCGTT+TGG - chr1.1:60050777-60050796 MS.gene005125:intron 45.0%
CAAACCTAGCAAAGAACTCG+AGG + chr1.1:60052039-60052058 None:intergenic 45.0%
CACACGACACACATGAGAAA+AGG + chr1.1:60052148-60052167 None:intergenic 45.0%
CGAGTTCTTTGCTAGGTTTG+AGG - chr1.1:60052039-60052058 MS.gene005125:intron 45.0%
CTAAGGATGAAGTAGAGAGC+AGG - chr1.1:60052491-60052510 MS.gene005125:CDS 45.0%
CTGCTCTCTACTTCATCCTT+AGG + chr1.1:60052493-60052512 None:intergenic 45.0%
GAGGTCGTTCGGTTTCAAAT+AGG - chr1.1:60052448-60052467 MS.gene005125:intron 45.0%
GGTATTTGCTCATGTGCCTA+AGG - chr1.1:60052474-60052493 MS.gene005125:CDS 45.0%
GGTCGTTCGGTTTCAAATAG+GGG - chr1.1:60052450-60052469 MS.gene005125:intron 45.0%
TAAGCGCTTGAGAGACTGTT+TGG - chr1.1:60050705-60050724 MS.gene005125:intron 45.0%
TCACAGATGAAGAGGTCGTT+CGG - chr1.1:60052437-60052456 MS.gene005125:intron 45.0%
TCCCGCAAAAGAACATGCTT+GGG - chr1.1:60050555-60050574 MS.gene005125:intron 45.0%
TGTTTGTGATGCGTAAAGGG+AGG - chr1.1:60049551-60049570 MS.gene005125:CDS 45.0%
TTATCCAAACGGGGCCTTTA+TGG + chr1.1:60050784-60050803 None:intergenic 45.0%
TTCAGACATCTGAGCAACCA+CGG + chr1.1:60052578-60052597 None:intergenic 45.0%
TTCCCGCAAAAGAACATGCT+TGG - chr1.1:60050554-60050573 MS.gene005125:intron 45.0%
! TATCGGGATGATTCTGGTTG+TGG + chr1.1:60050306-60050325 None:intergenic 45.0%
! TCAAGGAGATCTGCCTTGTT+TGG + chr1.1:60051948-60051967 None:intergenic 45.0%
!! AGGTAAAGCGAGTATCGAAG+GGG - chr1.1:60051596-60051615 MS.gene005125:intron 45.0%
AAAGGGAGGAGGATCCGAAA+CGG - chr1.1:60049565-60049584 MS.gene005125:CDS 50.0%
AAGAACATGCTTGGGGGTGA+TGG - chr1.1:60050563-60050582 MS.gene005125:intron 50.0%
AAGAACTCGAGGTAGGCAAC+AGG + chr1.1:60052028-60052047 None:intergenic 50.0%
CCCGCAAAAGAACATGCTTG+GGG - chr1.1:60050556-60050575 MS.gene005125:intron 50.0%
CCGCAAAAGAACATGCTTGG+GGG - chr1.1:60050557-60050576 MS.gene005125:intron 50.0%
CCTACCTCGAGTTCTTTGCT+AGG - chr1.1:60052032-60052051 MS.gene005125:intron 50.0%
CCTAGCAAAGAACTCGAGGT+AGG + chr1.1:60052035-60052054 None:intergenic 50.0%
CGTTCGGTTTCAAATAGGGG+AGG - chr1.1:60052453-60052472 MS.gene005125:intron 50.0%
CTCCCAGGTAACTTCCGTTT+CGG + chr1.1:60049582-60049601 None:intergenic 50.0%
GATCCGAAACGGAAGTTACC+TGG - chr1.1:60049576-60049595 MS.gene005125:CDS 50.0%
TGAGGAGGAGAAACAATCCG+TGG - chr1.1:60052558-60052577 MS.gene005125:intron 50.0%
TTGTGATGCGTAAAGGGAGG+AGG - chr1.1:60049554-60049573 MS.gene005125:CDS 50.0%
! CCCCAAGCATGTTCTTTTGC+GGG + chr1.1:60050559-60050578 None:intergenic 50.0%
! CCCCCAAGCATGTTCTTTTG+CGG + chr1.1:60050560-60050579 None:intergenic 50.0%
! GGGATGATTCTGGTTGTGGT+GGG + chr1.1:60050302-60050321 None:intergenic 50.0%
!! GCGAATCGCTGATAACGGAT+AGG + chr1.1:60050122-60050141 None:intergenic 50.0%
!! GGTAAAGCGAGTATCGAAGG+GGG - chr1.1:60051597-60051616 MS.gene005125:intron 50.0%
ATGGAGAGTTTGGCCCCGTT+TGG + chr1.1:60051807-60051826 None:intergenic 55.0%
CGAAACGGAAGTTACCTGGG+AGG - chr1.1:60049580-60049599 MS.gene005125:CDS 55.0%
GAGTTTGGCCCCGTTTGGAT+TGG + chr1.1:60051802-60051821 None:intergenic 55.0%
GTGTGTCGTGTGTGTCTGAG+AGG - chr1.1:60052156-60052175 MS.gene005125:intron 55.0%
! CGGGATGATTCTGGTTGTGG+TGG + chr1.1:60050303-60050322 None:intergenic 55.0%
GATGTTCTGCTGGTCCTCCC+AGG + chr1.1:60049597-60049616 None:intergenic 60.0%
GCGGGAAGTCTAGCACATCG+AGG + chr1.1:60050541-60050560 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 60049479 60052654 60049479 ID=MS.gene005125
chr1.1 mRNA 60049479 60052654 60049479 ID=MS.gene005125.t1;Parent=MS.gene005125
chr1.1 exon 60052646 60052654 60052646 ID=MS.gene005125.t1.exon1;Parent=MS.gene005125.t1
chr1.1 CDS 60052646 60052654 60052646 ID=cds.MS.gene005125.t1;Parent=MS.gene005125.t1
chr1.1 exon 60052454 60052564 60052454 ID=MS.gene005125.t1.exon2;Parent=MS.gene005125.t1
chr1.1 CDS 60052454 60052564 60052454 ID=cds.MS.gene005125.t1;Parent=MS.gene005125.t1
chr1.1 exon 60049479 60049739 60049479 ID=MS.gene005125.t1.exon3;Parent=MS.gene005125.t1
chr1.1 CDS 60049479 60049739 60049479 ID=cds.MS.gene005125.t1;Parent=MS.gene005125.t1
Gene Sequence

>MS.gene005125

ATGCAACAGGTAATTTGAAACCCTAAATTGAATTTTTGAGATTGCATTGTTTGATTTTGATTGAGACTCATTTGTTTGTGATGCGTAAAGGGAGGAGGATCCGAAACGGAAGTTACCTGGGAGGACCAGCAGAACATCAACAAATTCGGAAGGTTGAATAATCGCTTACACGAGCTACATGATGAGATCAAAATCGCTAAGGTAATCAATCTTTAATTTCAAATTTTGAGTTTTGCGTTAATCAAATTCAAAGGTTTTGGAATAGAAATGGGTCCAAAATCAAAGAGAATCCCTATATGAGTAGTTTATATCCTTTGCTACATGCTTTTTGTTGAGATAGTGCTATCTGTTTGGCAAGCCACATATCTGAGCTTATAAATTATGTCTTATAAGCTCATTTGACCACCTTAAGCTATAAGCTACTTTCTCACGAGCTTATAGCTTATATTTACTAGCTTGTAGTGTTTTTTTATAAGCTAATTCAAGTAGCTTATAACTTAAATCTTACAGCTTAAAGCTCATAGCTTATCATTTGTTTTATTTCAATTTTACCATTGTAGCCTTAATTGAAAAAAGTTATTAATTAAGCTAGTTCAACCGCTAATTTTATCAAACACTACGTACAAATTCAATCAGCTAGCCTATCCGTTATCAGCGATTCGCTATTTTATACCAAACAGAGACGTACAGTTTCTTCCACAAACAATTCTTATTCCCACACATACATGTATACATCACCTCATATGAACAAAGACAGAGTGTTTCATTTATTTTAAAGGAAAACAAAAATGGTTGGATGTGATTTCATAGATGAAATTAACCCACCACAACCAGAATCATCCCGATAGGAAATAGGAACCAGAATCATACAATATGAATCCGAGAAGGTTTCCTATTTGAATGGAAAAAACAAAAATACAAAATCTGGAGATTTAAGAACTCAACATACATTTTTATTTTGGAGTTCACCTCTTACTTTGAACTCCTAGCACTATAGAACTGATCAAATTTATGAAGAGTCATGTTTAAGAATAATAACTCGCAGCACAAAAGAAGCTTCCTCGATGTGCTAGACTTCCCGCAAAAGAACATGCTTGGGGGTGATGGTTATTAGAGAAAGCGTTCATCGGAAGAAAAAGAAATTAGCGGTAAGAAATACCGCTATATCGTTCTTATAGTTTTTGAGGAACCAGATAATTATCATTATTTGAGCTTAACTACTGGCATAAGCGCTTGAGAGACTGTTTGGGAACTTATGAAAACAACTTATGACATGTTCATAAGCCTGTTTTCAATTTATTTCCATAAAGGCCCCGTTTGGATAAATAGCTTATAGAGATGCTTATAACATTAGAGCTTATAGACTAGAGCTTACATCATAAGCTCTTATACTCGATAAGCTATTTATGTTTGGATATGTACACTAAAAAGTACTTACATAATTTGAAGTGTTTGGATGTGTCATTATATAAGCAATTAAAATAAAAAAAATATTTTTAATTTAAAAAAATTAAAGAAACAAACCACGACTAAAAAGCTGTAAAAAAACTATTAAATAATTTTAATATAAATAAAAAATTATCAATATTCGAATAGAAGAAAATTAACATTAAATAATCAAAACATCACCATTCACAAGAATTATATATATAATAATCAAACATATGTAAAAGTTTAATAGTCATAGTTGAAAAAAATATCATGTAATTTTCTCAAGTTGTGAGTTAAGTACTCTTTATACTTCTCACCTTTCTAATAAATTTGGTTGTAAAAAAAATATATCAGCAATAGGGAAAAAATTTATAAGAAAAAATGCACGAGAGATGAAACAAAACAACTATAAAAATTTACCTGGTATTTGTATTTGTATTGTGTCAACTCTCTATGTAAATTATTTTGAGAAATATGGTTGTATAAATTGAATGAGTTTAACATATATCATATATGTATATATACTTTGTAAAAAAAAATATATGTACATATAGATTAATTAAAGATACGTGTGTTAATTAAAGAATAAGGTATTCTAGTTAGGTATAAAAGACATGTATATTCCTTAAAAAAAAAAGACATGTATATAATAAATAAAATTAAAAAATATAGGCTTTGAAGAAAAAAGGTAAAGCGAGTATCGAAGGGGGTCATATATATATACTGGCATAATCTCCGCATAAGCTATGTGGTAAGCTCAAAATTATGGCTTATTAAAATATGGTATAAACGGCTTATGAAATTGTTATAAGCTATTTTTATTATTTACCCAAACGCATTTGAAAAGGCTTACGTTAGTAGATAAGCGCTCACATAAGCTCAAAATAAGCCAATCCAAACGGGGCCAAACTCTCCATGATAGCTTATGAAAACAAACAACTTATAGTTTATATGAAAACAGATTGACTTCATTTATCTTAAGTGCCTTTTATATAACAAGTGCTTATGACGTAAACTGCAAATAAAGTTGTTTATCCAAACAAGGCAGATCTCCTTGACTCAAGCAGAAAATAAATTATATATGAATTGTGTGATCTCGTCTGAAATAAGTTGAACCTGTTGCCTACCTCGAGTTCTTTGCTAGGTTTGAGGAAAAGGATGAGAGTGGACTGAAAAGTACAAGACAATGAAATGATTATATCCTCGAAAATCAATTATGTGGTTAACTACTTGACCCTTTTCTCATGTGTGTCGTGTGTGTCTGAGAGGTTACTTATACATATACAGATTTAAGAATTCATTACTTCATATGTGATGTCTTTCAACTTCTCGGTTTGGTTAAATAATATTTAGTTGTTTGAGGAAGCATCTGTTTTTTCAAGTCTTGTTACAGTTTTAGTTTCCCCTCCTTCTAAGCATTTTGCAATGACAAATCCATTGTTTTATATTTTGATGTTAATGCATGGAGATTTTGTTTGTCACAGGAATCAAATGATAATTTGGAGGATGCAAGCAATGAGTTAATTCTCACAGATGAAGAGGTCGTTCGGTTTCAAATAGGGGAGGTATTTGCTCATGTGCCTAAGGATGAAGTAGAGAGCAGGATAGAACAGATGCAAGAAGCGACAAGCCAGAAATTGGAAAAGCTTGAGGAGGAGAAACAATCCGTGGTTGCTCAGATGTCTGAACTGAAGAAAATTCTGTATGGGAAGTTTGGAGAATCTATCAATCTTGAGGAGGATTAG
Protein sequence

>MS.gene005125.t1

MQQGGGSETEVTWEDQQNINKFGRLNNRLHELHDEIKIAKESNDNLEDASNELILTDEEVVRFQIGEVFAHVPKDEVESRIEQMQEATSQKLEKLEEEKQSVVAQMSELKKILYGKFGESINLEED