AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003428

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003428.t1 AT3G02820 47.857 280 125 7 8 275 6 276 2.46e-78 239
MS.gene003428.t1 AT3G02820 47.857 280 125 7 8 275 23 293 3.68e-78 239

Find 78 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAGACTGGTTCCATTATT+TGG 0.093724 4.1:-28985243 None:intergenic
TAGGGTTTGGATTGGGATTT+GGG 0.153645 4.1:-28982835 None:intergenic
CTTCTCAGTTGTGCTACTTT+TGG 0.158577 4.1:-28985205 None:intergenic
GCGGTGGAGGTGGTGGTGTT+AGG 0.206154 4.1:-28982854 None:intergenic
TTAGGGTTTGGATTGGGATT+TGG 0.242699 4.1:-28982836 None:intergenic
GAGGTGGTGGTGTTAGGGTT+TGG 0.285661 4.1:-28982848 None:intergenic
GTAACAACCTGTTGCTGTTC+CGG 0.287274 4.1:-28982765 None:intergenic
AGAAGCTCGGGAGTGAGTTT+TGG 0.293857 4.1:-28982956 None:intergenic
CTCTTTCTCTCAATTCTCTA+AGG 0.296516 4.1:-28983917 None:intergenic
TCAAATGAAGTCCCAAATAA+TGG 0.319473 4.1:+28985231 MS.gene003428:CDS
AGGTGGATCGGCGGTGGAGG+TGG 0.322815 4.1:-28982864 None:intergenic
CTTCTCTCTGATGACGGCCT+TGG 0.344424 4.1:+28982977 MS.gene003428:CDS
GTGGTGTTAGGGTTTGGATT+GGG 0.352807 4.1:-28982842 None:intergenic
ACAGACTGGTTCCATTATTT+GGG 0.352932 4.1:-28985242 None:intergenic
CGGTGGAGGTGGTGGTGTTA+GGG 0.379415 4.1:-28982853 None:intergenic
TGCTGTTATCTCAGCACTGC+TGG 0.389855 4.1:-28985265 None:intergenic
GGAGGTAGGTGGATCGGCGG+TGG 0.391333 4.1:-28982870 None:intergenic
AGATTACCTTGTTCACCATC+TGG 0.397013 4.1:-28983996 None:intergenic
GGTAGGTGGATCGGCGGTGG+AGG 0.397936 4.1:-28982867 None:intergenic
TAGCTGTATCTGCAGAAAAC+GGG 0.413562 4.1:+28984558 MS.gene003428:intron
TGCTGCTACTAGGCGTGTTA+AGG 0.413675 4.1:+28983247 MS.gene003428:CDS
TGGATCGGCGGTGGAGGTGG+TGG 0.423688 4.1:-28982861 None:intergenic
TCAGGATCCGGAACAGCAAC+AGG 0.431444 4.1:+28982758 MS.gene003428:CDS
GGTGGTGTTAGGGTTTGGAT+TGG 0.441623 4.1:-28982843 None:intergenic
AGACTGTGGTATGCTGGCTT+TGG 0.446290 4.1:-28985343 None:intergenic
TCAACCTATTTGCTTCCATA+CGG 0.452181 4.1:-28985299 None:intergenic
GAGCCCGTATGGAAGCAAAT+AGG 0.454008 4.1:+28985295 MS.gene003428:CDS
CTCAGCACTGCTGGACAGAC+TGG 0.455206 4.1:-28985256 None:intergenic
TCTGGGATGTTGTCCACAAC+TGG 0.469784 4.1:-28983978 None:intergenic
GAGAAAGAGTTGCAAATGGC+GGG 0.470312 4.1:+28983931 MS.gene003428:CDS
CTAGGAAGACTGTGGTATGC+TGG 0.471259 4.1:-28985349 None:intergenic
GAAGAGAAAATGTCAGGATC+CGG 0.473828 4.1:+28982746 None:intergenic
ACCTCCACCTTCAAACCCTC+TGG 0.474558 4.1:+28982887 MS.gene003428:CDS
AGAAGAGAAGAGAAAATGTC+AGG 0.483482 4.1:+28982740 None:intergenic
CAACCTATTTGCTTCCATAC+GGG 0.491149 4.1:-28985298 None:intergenic
AGCAGAGCAGAGAGCCCGTA+TGG 0.494494 4.1:+28985284 MS.gene003428:CDS
TCTGGGTCCAGAGGGTTTGA+AGG 0.495023 4.1:-28982894 None:intergenic
TTGGGATTTGGGGCGGTGAA+AGG 0.495060 4.1:-28982824 None:intergenic
GTTTGGATTGGGATTTGGGG+CGG 0.497587 4.1:-28982831 None:intergenic
TTGAGAAAGTTGCTGCTACT+AGG 0.498945 4.1:+28983237 MS.gene003428:CDS
TCATCAGAGAGAAGAAGCTC+GGG 0.503800 4.1:-28982968 None:intergenic
GTCATCAGAGAGAAGAAGCT+CGG 0.508978 4.1:-28982969 None:intergenic
GATTACCTTGTTCACCATCT+GGG 0.514063 4.1:-28983995 None:intergenic
TGTTACAAATGCGGCAGACC+AGG 0.517761 4.1:+28982782 MS.gene003428:CDS
AGGGTTTGGATTGGGATTTG+GGG 0.518659 4.1:-28982834 None:intergenic
GGTTTGAAGGTGGAGGTAGG+TGG 0.525605 4.1:-28982881 None:intergenic
GAAGGTGGAGGTAGGTGGAT+CGG 0.539108 4.1:-28982876 None:intergenic
TATGGAAGCAAATAGGTTGA+AGG 0.541904 4.1:+28985302 MS.gene003428:CDS
TGCTTGACTTATACTCTGAC+TGG 0.544504 4.1:+28983165 MS.gene003428:CDS
ATAGGTTGAAGGCACTAGAA+AGG 0.545276 4.1:+28985313 MS.gene003428:CDS
AACTTCAAATACCACGGTCG+CGG 0.560735 4.1:+28983025 MS.gene003428:CDS
CCGTGGTATTTGAAGTTGCG+AGG 0.565970 4.1:-28983019 None:intergenic
CAGTCGCGTGACCAGTGACC+TGG 0.568375 4.1:-28982800 None:intergenic
GGTGGTTCACGCAACTTCGA+TGG 0.570994 4.1:-28983957 None:intergenic
GGGTCCAGAGGGTTTGAAGG+TGG 0.574431 4.1:-28982891 None:intergenic
TCCAGAGGGTTTGAAGGTGG+AGG 0.577332 4.1:-28982888 None:intergenic
AATTGATTGAAAGAGTAATA+CGG 0.577716 4.1:-28983203 None:intergenic
TTGAGAGAAAGAGTTGCAAA+TGG 0.578919 4.1:+28983927 MS.gene003428:CDS
AATGCGGCAGACCAGGTCAC+TGG 0.581408 4.1:+28982789 MS.gene003428:CDS
CAACCTCATGGCCGCGACCG+TGG 0.581868 4.1:-28983036 None:intergenic
GACGGCGGATCTGGGTCCAG+AGG 0.589738 4.1:-28982903 None:intergenic
CGTGGTATTTGAAGTTGCGA+GGG 0.591626 4.1:-28983018 None:intergenic
GAGGGTTTGAAGGTGGAGGT+AGG 0.595710 4.1:-28982884 None:intergenic
AACATCCCAGATGGTGAACA+AGG 0.595960 4.1:+28983990 MS.gene003428:CDS
ACGGCGGATCTGGGTCCAGA+GGG 0.600152 4.1:-28982902 None:intergenic
GAGCTTCTTCTCTCTGATGA+CGG 0.601771 4.1:+28982971 MS.gene003428:CDS
GGTGGAGGTAGGTGGATCGG+CGG 0.604040 4.1:-28982873 None:intergenic
AGAGAAAGAGTTGCAAATGG+CGG 0.622059 4.1:+28983930 MS.gene003428:CDS
AGAAAGAGTTGCAAATGGCG+GGG 0.622135 4.1:+28983932 MS.gene003428:CDS
GTTGCGTGAACCACCAGTTG+TGG 0.628493 4.1:+28983965 MS.gene003428:CDS
ATACCACGGTCGCGGCCATG+AGG 0.648998 4.1:+28983033 MS.gene003428:CDS
GGGATGTTGTCCACAACTGG+TGG 0.651915 4.1:-28983975 None:intergenic
AGCTGTATCTGCAGAAAACG+GGG 0.656668 4.1:+28984559 MS.gene003428:intron
GTTGTGGACAACATCCCAGA+TGG 0.677611 4.1:+28983981 MS.gene003428:CDS
CCTCGCAACTTCAAATACCA+CGG 0.686072 4.1:+28983019 MS.gene003428:CDS
GCAACAGGTTGTTACAAATG+CGG 0.691238 4.1:+28982773 MS.gene003428:CDS
AATAGCGAAGAACGTAACCA+AGG 0.694872 4.1:-28982994 None:intergenic
TATCAAAGCTAGGAAGACTG+TGG 0.695853 4.1:-28985357 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACAATTAATTAAGAAAA+AGG - chr4.1:28983075-28983094 None:intergenic 10.0%
!! AATATAAATAAAACTCACTT+CGG - chr4.1:28984684-28984703 None:intergenic 15.0%
!! AATAACGAAGCTTAAAAATA+AGG + chr4.1:28983425-28983444 MS.gene003428:intron 20.0%
!! AATTGATTGAAAGAGTAATA+CGG - chr4.1:28983206-28983225 None:intergenic 20.0%
!! TGAAAAAAGGAAAATTAAGA+GGG - chr4.1:28984258-28984277 None:intergenic 20.0%
!! TTGAAAAAAGGAAAATTAAG+AGG - chr4.1:28984259-28984278 None:intergenic 20.0%
!!! ACTTAATTGTTTGTTTTGTT+AGG + chr4.1:28983124-28983143 MS.gene003428:intron 20.0%
!!! ATGCATATTTTTCTTTCAAT+TGG + chr4.1:28983685-28983704 MS.gene003428:intron 20.0%
!!! ATTTTTAAGCTTCGTTATTA+TGG - chr4.1:28983424-28983443 None:intergenic 20.0%
!!! GAAATTTGTTAAATCACTTT+TGG + chr4.1:28984786-28984805 MS.gene003428:intron 20.0%
!!! TTCAATTACACTCAAAATTT+TGG - chr4.1:28983357-28983376 None:intergenic 20.0%
!!! TTTTTAAGCTTCGTTATTAT+GGG - chr4.1:28983423-28983442 None:intergenic 20.0%
! AAAGCATAAAAAAATAGCAG+AGG - chr4.1:28983745-28983764 None:intergenic 25.0%
! AACAAACAATTAAGTGATTG+CGG - chr4.1:28983119-28983138 None:intergenic 25.0%
! AAGCTTAAAAATAAGGACTT+TGG + chr4.1:28983432-28983451 MS.gene003428:intron 25.0%
! ACAATTATTAATTCAAACGC+AGG - chr4.1:28984072-28984091 None:intergenic 25.0%
! AGATGAGTACAAATATGATT+AGG + chr4.1:28983376-28983395 MS.gene003428:intron 25.0%
! ATGAGTACAAATATGATTAG+GGG + chr4.1:28983378-28983397 MS.gene003428:intron 25.0%
! ATTCGCATACAATTGAAAAA+AGG - chr4.1:28984271-28984290 None:intergenic 25.0%
! ATTTCCTTTCACAAAAGTAA+AGG - chr4.1:28984771-28984790 None:intergenic 25.0%
! CAGGTGATTAATTCAATAAA+TGG - chr4.1:28984195-28984214 None:intergenic 25.0%
! GATGAGTACAAATATGATTA+GGG + chr4.1:28983377-28983396 MS.gene003428:intron 25.0%
! GTTTATCACTAAATAGATGT+GGG - chr4.1:28985077-28985096 None:intergenic 25.0%
! TAAAAAAATAGCAGAGGAAT+CGG - chr4.1:28983739-28983758 None:intergenic 25.0%
! TAAAACACAAAGTTTGAACA+AGG - chr4.1:28983865-28983884 None:intergenic 25.0%
! TCAACATATATAAAGCCATA+GGG - chr4.1:28983813-28983832 None:intergenic 25.0%
! TGATTGATGGAAAATAGTTA+TGG - chr4.1:28983655-28983674 None:intergenic 25.0%
! TTGATCAAATTGTCAATTGT+TGG - chr4.1:28984325-28984344 None:intergenic 25.0%
!! AACTGATTCATATTTCAAAC+AGG + chr4.1:28984409-28984428 MS.gene003428:intron 25.0%
!! ACTGATTCATATTTCAAACA+GGG + chr4.1:28984410-28984429 MS.gene003428:intron 25.0%
!! ATGAAGGAATACATAGATTT+TGG + chr4.1:28984377-28984396 MS.gene003428:intron 25.0%
!! TAATTGTTTTCTTAAATCCG+TGG + chr4.1:28984085-28984104 MS.gene003428:intron 25.0%
!!! TTGAGTTGATAATTGTTTCT+AGG - chr4.1:28984149-28984168 None:intergenic 25.0%
AAAAGGAAAATTAAGAGGGA+AGG - chr4.1:28984254-28984273 None:intergenic 30.0%
AACAAATAGATCTTCTTACG+TGG - chr4.1:28984726-28984745 None:intergenic 30.0%
ACAACATCCTATAGTAAAGT+TGG + chr4.1:28984123-28984142 MS.gene003428:intron 30.0%
AGAAATCTAGAGACTTAAGA+AGG - chr4.1:28985015-28985034 None:intergenic 30.0%
ATCTGATGCAAAGTTTGTAA+CGG + chr4.1:28985113-28985132 MS.gene003428:intron 30.0%
CAATATTCCAGTGAACTATA+TGG - chr4.1:28984449-28984468 None:intergenic 30.0%
CATCATTAATAACACACTTC+TGG - chr4.1:28984908-28984927 None:intergenic 30.0%
CATTAGTTGAAGATGATTGA+TGG - chr4.1:28983668-28983687 None:intergenic 30.0%
CTCAACATATATAAAGCCAT+AGG - chr4.1:28983814-28983833 None:intergenic 30.0%
GATAAACCAAATCGTAAACA+AGG + chr4.1:28985090-28985109 MS.gene003428:intron 30.0%
GCATAATGTTAAAAGTTCAG+TGG + chr4.1:28983316-28983335 MS.gene003428:intron 30.0%
GGTTTATCACTAAATAGATG+TGG - chr4.1:28985078-28985097 None:intergenic 30.0%
TAATAACACACTTCTGGATA+AGG - chr4.1:28984902-28984921 None:intergenic 30.0%
TACTCACAAATTTGTGAGAA+TGG - chr4.1:28984833-28984852 None:intergenic 30.0%
TCAAATGAAGTCCCAAATAA+TGG + chr4.1:28985231-28985250 MS.gene003428:CDS 30.0%
TGAGATTAATTACAACCTCA+TGG - chr4.1:28983051-28983070 None:intergenic 30.0%
! AGTGTGTTATTAATGATGCA+AGG + chr4.1:28984910-28984929 MS.gene003428:intron 30.0%
! ATTACCTTCTCTTTTATTGC+AGG + chr4.1:28985137-28985156 MS.gene003428:intron 30.0%
! GTGTGTTATTAATGATGCAA+GGG + chr4.1:28984911-28984930 MS.gene003428:intron 30.0%
! TTTTGTTAGGTTCGTGATTT+GGG + chr4.1:28983137-28983156 MS.gene003428:intron 30.0%
!! TGAACCTTTACTTTTGTGAA+AGG + chr4.1:28984764-28984783 MS.gene003428:intron 30.0%
ACCATAGGTAGACAAAATGA+AGG + chr4.1:28984361-28984380 MS.gene003428:intron 35.0%
ATACAGCTAAACCCAATGTA+GGG - chr4.1:28984547-28984566 None:intergenic 35.0%
ATTTGATCAATGAAGCTCAC+TGG + chr4.1:28984335-28984354 MS.gene003428:intron 35.0%
CTCTTTCTCTCAATTCTCTA+AGG - chr4.1:28983920-28983939 None:intergenic 35.0%
GTGAACTATATGGAATCAGT+TGG - chr4.1:28984439-28984458 None:intergenic 35.0%
GTTCTCTAAGTTTACAACCA+CGG - chr4.1:28984105-28984124 None:intergenic 35.0%
TCAACCTATTTGCTTCCATA+CGG - chr4.1:28985302-28985321 None:intergenic 35.0%
TCACCTGTTATGAAGTTGTT+CGG + chr4.1:28984208-28984227 MS.gene003428:intron 35.0%
TTAAGCTTCGTTATTATGGG+TGG - chr4.1:28983420-28983439 None:intergenic 35.0%
TTAGCTGTATCTGCAGAAAA+CGG + chr4.1:28984557-28984576 MS.gene003428:intron 35.0%
TTAGTCTCGTGTATGCAAAA+AGG + chr4.1:28983584-28983603 MS.gene003428:intron 35.0%
TTCTAGGCCAACTTTACTAT+AGG - chr4.1:28984133-28984152 None:intergenic 35.0%
TTGAGAGAAAGAGTTGCAAA+TGG + chr4.1:28983927-28983946 MS.gene003428:CDS 35.0%
TTGCTAAGTATCCCTACATT+GGG + chr4.1:28984533-28984552 MS.gene003428:intron 35.0%
TTTCCGAACAACTTCATAAC+AGG - chr4.1:28984214-28984233 None:intergenic 35.0%
TTTGCTAAGTATCCCTACAT+TGG + chr4.1:28984532-28984551 MS.gene003428:intron 35.0%
! ACAGAAATCACATTTTGCAC+GGG - chr4.1:28985174-28985193 None:intergenic 35.0%
! ACAGACTGGTTCCATTATTT+GGG - chr4.1:28985245-28985264 None:intergenic 35.0%
! ACTGATTCCATATAGTTCAC+TGG + chr4.1:28984439-28984458 MS.gene003428:intron 35.0%
! CACAGAAATCACATTTTGCA+CGG - chr4.1:28985175-28985194 None:intergenic 35.0%
! TATGGAAGCAAATAGGTTGA+AGG + chr4.1:28985302-28985321 MS.gene003428:CDS 35.0%
! TCAGATCCTTGTTTACGATT+TGG - chr4.1:28985099-28985118 None:intergenic 35.0%
! TCATTTTGTCTACCTATGGT+TGG - chr4.1:28984361-28984380 None:intergenic 35.0%
! TCCTTCATTTTGTCTACCTA+TGG - chr4.1:28984365-28984384 None:intergenic 35.0%
! TTTTATTGCAGGAACCTTCT+CGG + chr4.1:28985148-28985167 MS.gene003428:intron 35.0%
!! ACATGCTGACTTCATAACAA+AGG - chr4.1:28985048-28985067 None:intergenic 35.0%
!! ATTGTTGGTTCTAATCTCTC+TGG - chr4.1:28984310-28984329 None:intergenic 35.0%
!! GTTTTGTTAGGTTCGTGATT+TGG + chr4.1:28983136-28983155 MS.gene003428:intron 35.0%
!!! TTCTCAGTTGTGCTACTTTT+GGG - chr4.1:28985207-28985226 None:intergenic 35.0%
AATAGCGAAGAACGTAACCA+AGG - chr4.1:28982997-28983016 None:intergenic 40.0%
AATCCCTTCTAACGGCAATT+GGG - chr4.1:28983508-28983527 None:intergenic 40.0%
AGAGAAAGAGTTGCAAATGG+CGG + chr4.1:28983930-28983949 MS.gene003428:CDS 40.0%
AGATTACCTTGTTCACCATC+TGG - chr4.1:28983999-28984018 None:intergenic 40.0%
CAACCTATTTGCTTCCATAC+GGG - chr4.1:28985301-28985320 None:intergenic 40.0%
CGACAGTTAATCCCTTCTAA+CGG - chr4.1:28983516-28983535 None:intergenic 40.0%
CGTTAGAAGGGATTAACTGT+CGG + chr4.1:28983514-28983533 MS.gene003428:intron 40.0%
GATACAGCTAAACCCAATGT+AGG - chr4.1:28984548-28984567 None:intergenic 40.0%
GATTACCTTGTTCACCATCT+GGG - chr4.1:28983998-28984017 None:intergenic 40.0%
GCAACAGGTTGTTACAAATG+CGG + chr4.1:28982773-28982792 MS.gene003428:CDS 40.0%
GGTTCCTGCAATAAAAGAGA+AGG - chr4.1:28985144-28985163 None:intergenic 40.0%
TAATCCCTTCTAACGGCAAT+TGG - chr4.1:28983509-28983528 None:intergenic 40.0%
TAGCTGTATCTGCAGAAAAC+GGG + chr4.1:28984558-28984577 MS.gene003428:intron 40.0%
TGCTTGACTTATACTCTGAC+TGG + chr4.1:28983165-28983184 MS.gene003428:CDS 40.0%
TTGAGAAAGTTGCTGCTACT+AGG + chr4.1:28983237-28983256 MS.gene003428:CDS 40.0%
! GACAGACTGGTTCCATTATT+TGG - chr4.1:28985246-28985265 None:intergenic 40.0%
! TAGGGTTTGGATTGGGATTT+GGG - chr4.1:28982838-28982857 None:intergenic 40.0%
! TCGACGTCGTTAACATTTTC+AGG - chr4.1:28984620-28984639 None:intergenic 40.0%
! TTAGGGTTTGGATTGGGATT+TGG - chr4.1:28982839-28982858 None:intergenic 40.0%
!! AGTTCAGTGGATTAGTGTTC+AGG + chr4.1:28983329-28983348 MS.gene003428:intron 40.0%
!! ATAGGTTGAAGGCACTAGAA+AGG + chr4.1:28985313-28985332 MS.gene003428:CDS 40.0%
!! TCAATTCTCTAAGGCTAGTC+TGG - chr4.1:28983911-28983930 None:intergenic 40.0%
!!! CTTCTCAGTTGTGCTACTTT+TGG - chr4.1:28985208-28985227 None:intergenic 40.0%
AACATCCCAGATGGTGAACA+AGG + chr4.1:28983990-28984009 MS.gene003428:CDS 45.0%
AACTTCAAATACCACGGTCG+CGG + chr4.1:28983025-28983044 MS.gene003428:CDS 45.0%
AGAAAGAGTTGCAAATGGCG+GGG + chr4.1:28983932-28983951 MS.gene003428:CDS 45.0%
AGCTGTATCTGCAGAAAACG+GGG + chr4.1:28984559-28984578 MS.gene003428:intron 45.0%
ATCCCTTCTAACGGCAATTG+GGG - chr4.1:28983507-28983526 None:intergenic 45.0%
CCTCGCAACTTCAAATACCA+CGG + chr4.1:28983019-28983038 MS.gene003428:CDS 45.0%
GAGAAAGAGTTGCAAATGGC+GGG + chr4.1:28983931-28983950 MS.gene003428:CDS 45.0%
GAGCTTCTTCTCTCTGATGA+CGG + chr4.1:28982971-28982990 MS.gene003428:CDS 45.0%
GTCATCAGAGAGAAGAAGCT+CGG - chr4.1:28982972-28982991 None:intergenic 45.0%
TCATCAGAGAGAAGAAGCTC+GGG - chr4.1:28982971-28982990 None:intergenic 45.0%
TTAGAGACTACTCGAACGTC+TGG + chr4.1:28984969-28984988 MS.gene003428:intron 45.0%
TTTACGCGTGAGTCAACTCT+AGG + chr4.1:28983468-28983487 MS.gene003428:intron 45.0%
! AGGGTTTGGATTGGGATTTG+GGG - chr4.1:28982837-28982856 None:intergenic 45.0%
! CGTGGTATTTGAAGTTGCGA+GGG - chr4.1:28983021-28983040 None:intergenic 45.0%
! GTAACAACCTGTTGCTGTTC+CGG - chr4.1:28982768-28982787 None:intergenic 45.0%
! GTGGTGTTAGGGTTTGGATT+GGG - chr4.1:28982845-28982864 None:intergenic 45.0%
!! AGGCTTTTTGGGCTTTTCGA+CGG - chr4.1:28982924-28982943 None:intergenic 45.0%
!! GAGGGAAGGTGTTCTAGTTA+AGG - chr4.1:28984240-28984259 None:intergenic 45.0%
!! TCTAAGGCTAGTCTGGTTAG+AGG - chr4.1:28983904-28983923 None:intergenic 45.0%
CTAACGGCAATTGGGGACAA+TGG - chr4.1:28983500-28983519 None:intergenic 50.0%
CTAGGAAGACTGTGGTATGC+TGG - chr4.1:28985352-28985371 None:intergenic 50.0%
GAGCCCGTATGGAAGCAAAT+AGG + chr4.1:28985295-28985314 MS.gene003428:CDS 50.0%
GCTCTGTATCTCTCTCCCTA+TGG + chr4.1:28983795-28983814 MS.gene003428:intron 50.0%
GGGAGAGAGATACAGAGCAA+TGG - chr4.1:28983793-28983812 None:intergenic 50.0%
GTCCCCAATTGCCGTTAGAA+GGG + chr4.1:28983502-28983521 MS.gene003428:intron 50.0%
GTTGTGGACAACATCCCAGA+TGG + chr4.1:28983981-28984000 MS.gene003428:CDS 50.0%
TGCTGCTACTAGGCGTGTTA+AGG + chr4.1:28983247-28983266 MS.gene003428:CDS 50.0%
TGCTGTTATCTCAGCACTGC+TGG - chr4.1:28985268-28985287 None:intergenic 50.0%
TGTCCCCAATTGCCGTTAGA+AGG + chr4.1:28983501-28983520 MS.gene003428:intron 50.0%
TGTTACAAATGCGGCAGACC+AGG + chr4.1:28982782-28982801 MS.gene003428:CDS 50.0%
TTAGGGGCCTGACAGTTACT+TGG + chr4.1:28983394-28983413 MS.gene003428:intron 50.0%
! AGAAGCTCGGGAGTGAGTTT+TGG - chr4.1:28982959-28982978 None:intergenic 50.0%
! CCGTGGTATTTGAAGTTGCG+AGG - chr4.1:28983022-28983041 None:intergenic 50.0%
! GGTGGTGTTAGGGTTTGGAT+TGG - chr4.1:28982846-28982865 None:intergenic 50.0%
! GTTTGGATTGGGATTTGGGG+CGG - chr4.1:28982834-28982853 None:intergenic 50.0%
! TCTGGGATGTTGTCCACAAC+TGG - chr4.1:28983981-28984000 None:intergenic 50.0%
!! AGACTGTGGTATGCTGGCTT+TGG - chr4.1:28985346-28985365 None:intergenic 50.0%
!! CTTTTTGGGCTTTTCGACGG+CGG - chr4.1:28982921-28982940 None:intergenic 50.0%
ACCTCCACCTTCAAACCCTC+TGG + chr4.1:28982887-28982906 MS.gene003428:CDS 55.0%
ACGGCAATTGGGGACAATGG+CGG - chr4.1:28983497-28983516 None:intergenic 55.0%
GAAGCTCACTGGCCAACCAT+AGG + chr4.1:28984346-28984365 MS.gene003428:intron 55.0%
GAAGGTGGAGGTAGGTGGAT+CGG - chr4.1:28982879-28982898 None:intergenic 55.0%
GAGGGTTTGAAGGTGGAGGT+AGG - chr4.1:28982887-28982906 None:intergenic 55.0%
GGGATGTTGTCCACAACTGG+TGG - chr4.1:28983978-28983997 None:intergenic 55.0%
GGTGGTTCACGCAACTTCGA+TGG - chr4.1:28983960-28983979 None:intergenic 55.0%
GGTTTGAAGGTGGAGGTAGG+TGG - chr4.1:28982884-28982903 None:intergenic 55.0%
GTTGCGTGAACCACCAGTTG+TGG + chr4.1:28983965-28983984 MS.gene003428:CDS 55.0%
TCAGGATCCGGAACAGCAAC+AGG + chr4.1:28982758-28982777 MS.gene003428:CDS 55.0%
TCCAGAGGGTTTGAAGGTGG+AGG - chr4.1:28982891-28982910 None:intergenic 55.0%
TCTGGGTCCAGAGGGTTTGA+AGG - chr4.1:28982897-28982916 None:intergenic 55.0%
TGGGTGGCCAAGTAACTGTC+AGG - chr4.1:28983404-28983423 None:intergenic 55.0%
! ACATTTTGCACGGGCCGAGA+AGG - chr4.1:28985165-28985184 None:intergenic 55.0%
! ACGGGTACGTGGAGGCTTTT+TGG - chr4.1:28982936-28982955 None:intergenic 55.0%
! CGGGTACGTGGAGGCTTTTT+GGG - chr4.1:28982935-28982954 None:intergenic 55.0%
! GAGGTGGTGGTGTTAGGGTT+TGG - chr4.1:28982851-28982870 None:intergenic 55.0%
! TTGGGATTTGGGGCGGTGAA+AGG - chr4.1:28982827-28982846 None:intergenic 55.0%
!! CTTCTCTCTGATGACGGCCT+TGG + chr4.1:28982977-28982996 MS.gene003428:CDS 55.0%
!!! AGTTTTGGACGGGTACGTGG+AGG - chr4.1:28982944-28982963 None:intergenic 55.0%
!!! CTCGGGAGTGAGTTTTGGAC+GGG - chr4.1:28982954-28982973 None:intergenic 55.0%
!!! GCTCGGGAGTGAGTTTTGGA+CGG - chr4.1:28982955-28982974 None:intergenic 55.0%
!!! GTGAGTTTTGGACGGGTACG+TGG - chr4.1:28982947-28982966 None:intergenic 55.0%
AATGCGGCAGACCAGGTCAC+TGG + chr4.1:28982789-28982808 MS.gene003428:CDS 60.0%
AGCAGAGCAGAGAGCCCGTA+TGG + chr4.1:28985284-28985303 MS.gene003428:CDS 60.0%
CTCAGCACTGCTGGACAGAC+TGG - chr4.1:28985259-28985278 None:intergenic 60.0%
GGGTCCAGAGGGTTTGAAGG+TGG - chr4.1:28982894-28982913 None:intergenic 60.0%
! CGGTGGAGGTGGTGGTGTTA+GGG - chr4.1:28982856-28982875 None:intergenic 60.0%
! GGCTTTTCGACGGCGGATCT+GGG - chr4.1:28982914-28982933 None:intergenic 60.0%
ACGGCGGATCTGGGTCCAGA+GGG - chr4.1:28982905-28982924 None:intergenic 65.0%
ATACCACGGTCGCGGCCATG+AGG + chr4.1:28983033-28983052 MS.gene003428:CDS 65.0%
CAGTCGCGTGACCAGTGACC+TGG - chr4.1:28982803-28982822 None:intergenic 65.0%
GGTGGAGGTAGGTGGATCGG+CGG - chr4.1:28982876-28982895 None:intergenic 65.0%
! GCGGTGGAGGTGGTGGTGTT+AGG - chr4.1:28982857-28982876 None:intergenic 65.0%
! GGGCTTTTCGACGGCGGATC+TGG - chr4.1:28982915-28982934 None:intergenic 65.0%
AGGTGGATCGGCGGTGGAGG+TGG - chr4.1:28982867-28982886 None:intergenic 70.0%
CAACCTCATGGCCGCGACCG+TGG - chr4.1:28983039-28983058 None:intergenic 70.0%
GACGGCGGATCTGGGTCCAG+AGG - chr4.1:28982906-28982925 None:intergenic 70.0%
GGAGGTAGGTGGATCGGCGG+TGG - chr4.1:28982873-28982892 None:intergenic 70.0%
GGTAGGTGGATCGGCGGTGG+AGG - chr4.1:28982870-28982889 None:intergenic 70.0%
TGGATCGGCGGTGGAGGTGG+TGG - chr4.1:28982864-28982883 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28982755 28985371 28982755 ID=MS.gene003428
chr4.1 mRNA 28982755 28985371 28982755 ID=MS.gene003428.t1;Parent=MS.gene003428
chr4.1 exon 28982755 28983054 28982755 ID=MS.gene003428.t1.exon1;Parent=MS.gene003428.t1
chr4.1 CDS 28982755 28983054 28982755 ID=cds.MS.gene003428.t1;Parent=MS.gene003428.t1
chr4.1 exon 28983146 28983268 28983146 ID=MS.gene003428.t1.exon2;Parent=MS.gene003428.t1
chr4.1 CDS 28983146 28983268 28983146 ID=cds.MS.gene003428.t1;Parent=MS.gene003428.t1
chr4.1 exon 28983912 28984011 28983912 ID=MS.gene003428.t1.exon3;Parent=MS.gene003428.t1
chr4.1 CDS 28983912 28984011 28983912 ID=cds.MS.gene003428.t1;Parent=MS.gene003428.t1
chr4.1 exon 28984573 28984685 28984573 ID=MS.gene003428.t1.exon4;Parent=MS.gene003428.t1
chr4.1 CDS 28984573 28984685 28984573 ID=cds.MS.gene003428.t1;Parent=MS.gene003428.t1
chr4.1 exon 28985159 28985371 28985159 ID=MS.gene003428.t1.exon5;Parent=MS.gene003428.t1
chr4.1 CDS 28985159 28985371 28985159 ID=cds.MS.gene003428.t1;Parent=MS.gene003428.t1
Gene Sequence

>MS.gene003428

ATGTCAGGATCCGGAACAGCAACAGGTTGTTACAAATGCGGCAGACCAGGTCACTGGTCACGCGACTGTCCTTTCACCGCCCCAAATCCCAATCCAAACCCTAACACCACCACCTCCACCGCCGATCCACCTACCTCCACCTTCAAACCCTCTGGACCCAGATCCGCCGTCGAAAAGCCCAAAAAGCCTCCACGTACCCGTCCAAAACTCACTCCCGAGCTTCTTCTCTCTGATGACGGCCTTGGTTACGTTCTTCGCTATTTCCCTCGCAACTTCAAATACCACGGTCGCGGCCATGAGGTTGTAATTAATCTCATCCTTTTTCTTAATTAATTGTTAATTCATTTTCGATTAATATTAACCGCAATCACTTAATTGTTTGTTTTGTTAGGTTCGTGATTTGGGTAAGTTGCTTGACTTATACTCTGACTGGCACTCTCGTTTGCTACCGTATTACTCTTTCAATCAATTTGTCAATAAAGTTGAGAAAGTTGCTGCTACTAGGCGTGTTAAGGTTAGTGCTTTCTTCAATGTTGAATTGTACATAATTATTTAAATTTAGCATAATGTTAAAAGTTCAGTGGATTAGTGTTCAGGTGCCAAAATTTTGAGTGTAATTGAAGATGAGTACAAATATGATTAGGGGCCTGACAGTTACTTGGCCACCCATAATAACGAAGCTTAAAAATAAGGACTTTGGCTGCAACTATTCTTTTACGCGTGAGTCAACTCTAGGAGACCGCCATTGTCCCCAATTGCCGTTAGAAGGGATTAACTGTCGGCTATTTGCTTGTTGTAGAAAGTAGTTTCTTGCTGTTGAACATGCATGTTAGTCTCGTGTATGCAAAAAGGATGTGACATTGACTTATTTAATTTATAAACTCAACATCTGTGTTTCCATAACTATTTTCCATCAATCATCTTCAACTAATGCATATTTTTCTTTCAATTGGACATGCATGATACAAGTATATAAGTGTTCCGATTCCTCTGCTATTTTTTTATGCTTTTATACACATACATTGATATGTTTTGCCATTGCTCTGTATCTCTCTCCCTATGGCTTTATATATGTTGAGAGAGTGTGTTTGTTGCATACTTGCATGTCCTTGTTCAAACTTTGTGTTTTAACATTTATTTGTACGTCCTCTAACCAGACTAGCCTTAGAGAATTGAGAGAAAGAGTTGCAAATGGCGGGGATCCATCGAAGTTGCGTGAACCACCAGTTGTGGACAACATCCCAGATGGTGAACAAGGTAATCTACTATCAATGTATTTGCACTTCGCTATCAACTCAAACTCAAAATACATTACCTGCGTTTGAATTAATAATTGTTTTCTTAAATCCGTGGTTGTAAACTTAGAGAACAACATCCTATAGTAAAGTTGGCCTAGAAACAATTATCAACTCAACAAGTTAACAATGTTGACAAGTACCATTTATTGAATTAATCACCTGTTATGAAGTTGTTCGGAAATATCCTTAACTAGAACACCTTCCCTCTTAATTTTCCTTTTTTCAATTGTATGCGAATTTTTAAATGTTAATTTCCAGAGAGATTAGAACCAACAATTGACAATTTGATCAATGAAGCTCACTGGCCAACCATAGGTAGACAAAATGAAGGAATACATAGATTTTGGAAAACTTCGAACTGATTCATATTTCAAACAGGGTAGCCAACTGATTCCATATAGTTCACTGGAATATTGCTATAAAGTTCAACTAAAAGAACTAATTAGATTTTATTTATTTTATTGACTTTATTTAGTATCTTTGCTAAGTATCCCTACATTGGGTTTAGCTGTATCTGCAGAAAACGGGGAAGCAAGTCATCAAGATAATGAGATGTTTGCAGAACCTGAAAATGTTAACGACGTCGACATTCAAGAAGATTTGTTCAATGATATATATGATAAAGCTACCGAAGTGAGTTTTATTTATATTAAACATTGTTCTTTTATCACCACGTAAGAAGATCTATTTGTTTCTTTGTTAAAACACATCTGAACCTTTACTTTTGTGAAAGGAAATTTGTTAAATCACTTTTGGTAACTTGTCTTCTATATATGACCATTCTCACAAATTTGTGAGTAATCTGAATTGTTTAGCAAATGTTTGTCAGTCAGATTTAGTTTTTATCCTTATCCAGAAGTGTGTTATTAATGATGCAAGGGTAACATTAATCTCTCAAGATTTTTGTATTCTTTCATTAGAGACTACTCGAACGTCTGGTTTATACTTAATTTTTCTTTCCTTCTTAAGTCTCTAGATTTCTTGTAATTATTCCTTTGTTATGAAGTCAGCATGTAAGATGCCCACATCTATTTAGTGATAAACCAAATCGTAAACAAGGATCTGATGCAAAGTTTGTAACGGCATTACCTTCTCTTTTATTGCAGGAACCTTCTCGGCCCGTGCAAAATGTGATTTCTGTGAGTACTGATCCCAAAAGTAGCACAACTGAGAAGACATCAAATGAAGTCCCAAATAATGGAACCAGTCTGTCCAGCAGTGCTGAGATAACAGCAGAGCAGAGAGCCCGTATGGAAGCAAATAGGTTGAAGGCACTAGAAAGGCGTGCAGCCAAAGCCAGCATACCACAGTCTTCCTAG
Protein sequence

>MS.gene003428.t1

MSGSGTATGCYKCGRPGHWSRDCPFTAPNPNPNPNTTTSTADPPTSTFKPSGPRSAVEKPKKPPRTRPKLTPELLLSDDGLGYVLRYFPRNFKYHGRGHEVRDLGKLLDLYSDWHSRLLPYYSFNQFVNKVEKVAATRRVKTSLRELRERVANGGDPSKLREPPVVDNIPDGEQENGEASHQDNEMFAEPENVNDVDIQEDLFNDIYDKATEEPSRPVQNVISVSTDPKSSTTEKTSNEVPNNGTSLSSSAEITAEQRARMEANRLKALERRAAKASIPQSS