AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77076

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77076.t1 MTR_7g029115 91.238 719 46 1 52 770 1 702 0.0 1295
MS.gene77076.t1 MTR_3g115250 70.891 797 180 9 3 754 1 790 0.0 1085
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77076.t1 AT1G42440 58.020 798 280 11 3 753 1 790 0.0 914

Find 157 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATGCCTTTCTGTATTT+AGG 0.205408 7.3:-71088432 MS.gene77076:CDS
AGAGGATGTTAAATTCTTTA+AGG 0.213886 7.3:-71082442 MS.gene77076:intron
AAGAATCTGTCCTTCATTTC+AGG 0.226133 7.3:-71083350 MS.gene77076:intron
AGTCTATTCGGAATTCTATA+TGG 0.254313 7.3:-71083699 MS.gene77076:CDS
GAATCTGAAGCAGAGCTTAA+AGG 0.271947 7.3:+71082003 None:intergenic
TTTGTTCAGGTTCATGTATC+AGG 0.277316 7.3:-71087569 MS.gene77076:intron
TTTGTTCCATCAGCCTCATT+TGG 0.281858 7.3:+71084740 None:intergenic
TAACTCTTCCTTGGATTTGA+TGG 0.304439 7.3:+71083175 None:intergenic
GACGCGGTTTGCAAAATATA+AGG 0.305864 7.3:-71083728 MS.gene77076:CDS
CGATATTCTAATCTTATATT+CGG 0.320602 7.3:+71088981 None:intergenic
CTTTCTGTATTTAGGTCTCT+TGG 0.321593 7.3:-71088424 MS.gene77076:CDS
CCGTCAGAATAACGCGCTTC+AGG 0.325079 7.3:+71082827 None:intergenic
TACCAACCGGTTCTTTAATT+CGG 0.327324 7.3:+71082257 None:intergenic
TAATATTTCATGTTGGGTTT+CGG 0.340526 7.3:-71083154 MS.gene77076:CDS
TCCGGGAACATCAGAATATC+AGG 0.340978 7.3:-71084669 MS.gene77076:intron
TCTCAGTACATTGATTCGTT+TGG 0.345900 7.3:-71088457 MS.gene77076:CDS
GATGAAGAGGAGTCAGATTA+TGG 0.348630 7.3:-71084215 MS.gene77076:CDS
GCACCAGAAGTAGCTGCTTT+GGG 0.354608 7.3:-71082883 MS.gene77076:CDS
TTAAAGAATTTAACATCCTC+TGG 0.355436 7.3:+71082444 None:intergenic
ATGAAATATTAACTCTTCCT+TGG 0.358498 7.3:+71083166 None:intergenic
TCGATTACAGAGAAATAAAA+TGG 0.359523 7.3:-71090128 MS.gene77076:intron
AAAAGATGGTTTGGCCTCTT+GGG 0.360897 7.3:+71082027 None:intergenic
GGAAAGGGAGAAAATGAAAT+AGG 0.361457 7.3:+71082940 None:intergenic
GAGCCCAAAGCAGCTACTTC+TGG 0.367122 7.3:+71082880 None:intergenic
TGCACCAGAAGTAGCTGCTT+TGG 0.374949 7.3:-71082884 MS.gene77076:CDS
GGAGGATCAAAATGCAGTTT+AGG 0.379707 7.3:-71081968 None:intergenic
GTTGTAGGTTGCTGATCTAC+TGG 0.383059 7.3:-71088521 MS.gene77076:intron
GGTTTCGGCAATTTGTTTGC+AGG 0.386760 7.3:-71083139 MS.gene77076:intron
TTTGCAAACCGCGTCCTAGC+TGG 0.390492 7.3:+71083736 None:intergenic
TTGTTCCATCAGCCTCATTT+GGG 0.391052 7.3:+71084741 None:intergenic
CGAATATAAGATTAGAATAT+CGG 0.392691 7.3:-71088980 MS.gene77076:intron
TTCCAGTTGGCTCATACATT+AGG 0.393373 7.3:-71083473 MS.gene77076:CDS
TTCAGATTCACTTCCATGTA+TGG 0.394866 7.3:-71081989 MS.gene77076:CDS
TTGAATTCGCATGAGAAAAT+CGG 0.400083 7.3:+71083033 None:intergenic
GGTTCCAGATCGTCAAAATC+TGG 0.400296 7.3:-71085041 MS.gene77076:CDS
TCTTGCTAAAGCTTTAGAAA+TGG 0.404433 7.3:-71083520 MS.gene77076:CDS
ACCTGATATTCTGATGTTCC+CGG 0.405785 7.3:+71084668 None:intergenic
TACAGATACGTGAACAGAAA+AGG 0.406730 7.3:-71089511 MS.gene77076:intron
AAGAGTTAATATTTCATGTT+GGG 0.407651 7.3:-71083160 MS.gene77076:CDS
ATAAGGGACTCAAGTCTATT+CGG 0.408251 7.3:-71083711 MS.gene77076:CDS
CAACTGGAACACAGTCTTCT+CGG 0.421252 7.3:+71083487 None:intergenic
ATCTTACCTCATGGCTCATA+AGG 0.433597 7.3:-71087990 MS.gene77076:intron
ATACATTAGGCTGCATATCA+TGG 0.436002 7.3:-71083460 MS.gene77076:CDS
GAAGATACAAGATGAACTTA+AGG 0.436203 7.3:-71084022 MS.gene77076:CDS
GCATCTCATCTGAACCCATT+AGG 0.438120 7.3:+71087471 None:intergenic
TATACCAAACGCAGTATTCG+TGG 0.443764 7.3:-71082280 MS.gene77076:CDS
TTTACGTTTCGACACTCGTT+GGG 0.446883 7.3:+71082494 None:intergenic
ACTGTTGGTATTGCAAATCC+TGG 0.447961 7.3:+71089012 None:intergenic
GCTCTCTTGAGGATTATTGT+AGG 0.451656 7.3:+71082919 None:intergenic
TGTCAAGAATGCTGGAGAGC+AGG 0.453277 7.3:-71085008 MS.gene77076:intron
TGAAAACTGTTGATCGAGAT+AGG 0.455302 7.3:-71082855 MS.gene77076:CDS
TCTACTCTCATGCATGGAAA+TGG 0.456856 7.3:-71088639 MS.gene77076:CDS
AGCAGACCACATGCTGTAAC+CGG 0.458582 7.3:+71083387 None:intergenic
GATATAGTTGTCAAGAATGC+TGG 0.465249 7.3:-71085016 MS.gene77076:CDS
TCGGCCACGAATACTGCGTT+TGG 0.468726 7.3:+71082276 None:intergenic
AACTCTTCCTTGGATTTGAT+GGG 0.469335 7.3:+71083176 None:intergenic
GTCTATTCGGAATTCTATAT+GGG 0.472019 7.3:-71083698 MS.gene77076:CDS
TTGAGGATTATTGTAGGAAA+GGG 0.473102 7.3:+71082925 None:intergenic
GAATTCTATATGGGATCCTA+TGG 0.478428 7.3:-71083689 MS.gene77076:intron
CAGCCTCTCTGTGAATCAAA+TGG 0.478691 7.3:-71087654 MS.gene77076:intron
AAAGATAACCAGCGAGAAGA+AGG 0.482820 7.3:+71087684 None:intergenic
AACATACCGTGTGTACCAAC+CGG 0.484373 7.3:+71082244 None:intergenic
CATAACTATACCTGAAATGA+AGG 0.486849 7.3:+71083340 None:intergenic
TCGTGGCCGAATTAAAGAAC+CGG 0.487872 7.3:-71082263 MS.gene77076:CDS
ACAGATACGTGAACAGAAAA+GGG 0.490199 7.3:-71089510 MS.gene77076:intron
TCTTCTAAATCTTCTCTCAA+AGG 0.496466 7.3:-71090298 MS.gene77076:intron
GGTTACAGCATGTGGTCTGC+TGG 0.496699 7.3:-71083385 MS.gene77076:CDS
CTTTGCTCTATGTGCTTCTG+TGG 0.498498 7.3:-71089097 MS.gene77076:CDS
AATGGCTTAAAGTTCCTCAT+TGG 0.499435 7.3:-71088024 MS.gene77076:CDS
CGATGAAGTGGATACGCCAT+TGG 0.501229 7.3:-71083764 MS.gene77076:CDS
GAAGAGTTAATATTTCATGT+TGG 0.502923 7.3:-71083161 MS.gene77076:CDS
TTTACTTGAGTTCAAAAGAA+GGG 0.502985 7.3:+71087502 None:intergenic
AGATCATCCTTTGTATCAGC+TGG 0.504240 7.3:+71088200 None:intergenic
CTTTACGTTTCGACACTCGT+TGG 0.505082 7.3:+71082493 None:intergenic
TTGCAAACCGCGTCCTAGCT+GGG 0.505760 7.3:+71083737 None:intergenic
CTTGAGGATTATTGTAGGAA+AGG 0.506116 7.3:+71082924 None:intergenic
CTGAAGCGCGTTATTCTGAC+GGG 0.506308 7.3:-71082826 MS.gene77076:CDS
CTTCATAATTGCAGTTCTTG+TGG 0.506384 7.3:-71088060 MS.gene77076:intron
CAGGTTCATGTATCAGGTGC+TGG 0.509397 7.3:-71087563 MS.gene77076:CDS
CGTTTGGTATAAATCTCTAC+AGG 0.510956 7.3:+71082292 None:intergenic
ACGCGGTTTGCAAAATATAA+GGG 0.514778 7.3:-71083727 MS.gene77076:CDS
ACAATAATCCTCAAGAGAGC+TGG 0.517105 7.3:-71082916 MS.gene77076:CDS
ATGGAGAGGTTTCTCCATGC+TGG 0.517625 7.3:-71082991 MS.gene77076:CDS
CAATGGCGTATCCACTTCAT+CGG 0.518162 7.3:+71083765 None:intergenic
AAGAAAGAGAAAGCTTCCTC+CGG 0.519515 7.3:-71084687 MS.gene77076:CDS
GGATAACATTTACAATCCTC+AGG 0.523806 7.3:+71088221 None:intergenic
CATCCTCTCCAGCTCTCTTG+AGG 0.525164 7.3:+71082908 None:intergenic
TGTAGAGTTTCCCGATGAAG+TGG 0.533637 7.3:-71083776 MS.gene77076:intron
AAATGCACCCTTCTTCTCGC+TGG 0.534053 7.3:-71087692 MS.gene77076:CDS
AGCCTAATGTATGAGCCAAC+TGG 0.534711 7.3:+71083471 None:intergenic
ACAGAACGCAACGTTGGTAA+AGG 0.539454 7.3:-71090163 MS.gene77076:CDS
AATGGCGTATCCACTTCATC+GGG 0.540957 7.3:+71083766 None:intergenic
TGTGGCTCTTTAAAGAGCAA+TGG 0.548868 7.3:-71088042 MS.gene77076:CDS
TAACTTTGAAGCAACACCGC+AGG 0.556209 7.3:+71083431 None:intergenic
AAGTGACAAAGTTTCAATGA+TGG 0.557684 7.3:-71084159 MS.gene77076:intron
GTCCTAGCTGGGACATCCAA+TGG 0.559338 7.3:+71083748 None:intergenic
GAACTTCAACTTTACACAGT+CGG 0.559401 7.3:+71087534 None:intergenic
GATTCTTCTACAGTACTCCC+AGG 0.560569 7.3:-71089030 MS.gene77076:CDS
AAAGATGGTTTGGCCTCTTG+GGG 0.560815 7.3:+71082028 None:intergenic
AGAAGCTCATGCTGCAGATG+AGG 0.562053 7.3:-71083992 MS.gene77076:CDS
GATATTGATGATTGTACTGA+TGG 0.562305 7.3:-71087725 MS.gene77076:CDS
TATGGCATTGAAGATGATGA+TGG 0.567288 7.3:-71084197 MS.gene77076:CDS
GTTCAAGAACTCCGTTGAGA+AGG 0.567385 7.3:+71082091 None:intergenic
ATGTTATCCAGCTGATACAA+AGG 0.568034 7.3:-71088207 MS.gene77076:CDS
TTACCAGACACGCCCAAATG+AGG 0.568389 7.3:-71084753 MS.gene77076:intron
TATTCGGAAGAAGCAACTGT+TGG 0.570763 7.3:+71088997 None:intergenic
AGAAAGAGAAAGCTTCCTCC+GGG 0.574353 7.3:-71084686 MS.gene77076:CDS
TTACTTGAGTTCAAAAGAAG+GGG 0.574385 7.3:+71087503 None:intergenic
TGGATGTCCCAGCTAGGACG+CGG 0.575299 7.3:-71083744 MS.gene77076:CDS
CAAGAGACCTAAATACAGAA+AGG 0.576022 7.3:+71088425 None:intergenic
CATAGCAGAATGGGAATGAG+TGG 0.578376 7.3:-71090361 None:intergenic
AGATGAGATGCACGATGCGA+AGG 0.582163 7.3:-71087459 MS.gene77076:intron
CCTGAAGCGCGTTATTCTGA+CGG 0.584069 7.3:-71082827 MS.gene77076:CDS
CGCCATTGGATGTCCCAGCT+AGG 0.584453 7.3:-71083750 MS.gene77076:CDS
TGGTGATCTACTCTCATGCA+TGG 0.586716 7.3:-71088645 MS.gene77076:CDS
ACACGCCCAAATGAGGCTGA+TGG 0.587587 7.3:-71084746 MS.gene77076:CDS
GAAGCTCATGCTGCAGATGA+GGG 0.590665 7.3:-71083991 MS.gene77076:intron
AACTTTGAAGCAACACCGCA+GGG 0.597089 7.3:+71083432 None:intergenic
CTGTTGGTATTGCAAATCCT+GGG 0.598130 7.3:+71089013 None:intergenic
ATGGGATGAATTCGATGAAG+AGG 0.601876 7.3:-71084228 MS.gene77076:CDS
CTCATGCATGGAAATGGTCA+AGG 0.602514 7.3:-71088633 MS.gene77076:intron
ACACTACCGGTTACAGCATG+TGG 0.608337 7.3:-71083393 MS.gene77076:CDS
AAATGAGGCTGATGGAACAA+AGG 0.609177 7.3:-71084738 MS.gene77076:CDS
AACTCAACGATCTTACCTCA+TGG 0.611394 7.3:-71087999 MS.gene77076:CDS
AAACGCAGACAAGAACAAGA+TGG 0.613118 7.3:-71083010 MS.gene77076:CDS
AATCCTCAAGAGAGCTGGAG+AGG 0.613954 7.3:-71082911 MS.gene77076:CDS
CGAGAAGACTGTGTTCCAGT+TGG 0.614137 7.3:-71083486 MS.gene77076:CDS
CGAACGAAGAGAACAGTGCT+TGG 0.617692 7.3:+71088397 None:intergenic
TCACGCGCCCATCAAATCCA+AGG 0.619601 7.3:-71083183 MS.gene77076:CDS
GAAGAGAAACGAGACTTGTG+AGG 0.623454 7.3:+71090316 None:intergenic
AGATTCACTTCCATGTATGG+AGG 0.623829 7.3:-71081986 MS.gene77076:CDS
ACAATCCTCAGGAAATTCTG+AGG 0.624314 7.3:+71088232 None:intergenic
TATACTTGCAAAGACACTAC+CGG 0.630488 7.3:-71083406 MS.gene77076:CDS
AAGATAACCAGCGAGAAGAA+GGG 0.631208 7.3:+71087685 None:intergenic
TCTTGCCTCAGAATTTCCTG+AGG 0.643770 7.3:-71088237 MS.gene77076:CDS
ATAGCAGAATGGGAATGAGT+GGG 0.646682 7.3:-71090360 None:intergenic
CAGGTACTAAAAGCACCTCA+TGG 0.646730 7.3:-71088665 MS.gene77076:CDS
AAAGAACCGGTTGGTACACA+CGG 0.647276 7.3:-71082250 MS.gene77076:intron
TTCACATGAAGACTTTGCCG+AGG 0.648716 7.3:+71088489 None:intergenic
GATCGTTGAGTTCTCCAATG+AGG 0.650248 7.3:+71088010 None:intergenic
GCAATGAAGTGCCTTCTCAA+CGG 0.651139 7.3:-71082102 MS.gene77076:CDS
TTACCATTTGATTCACAGAG+AGG 0.651783 7.3:+71087651 None:intergenic
AGATGATGATGGAGATTCTG+AGG 0.652330 7.3:-71084186 MS.gene77076:CDS
ACAAACGTGCATTCCCCAAG+AGG 0.657234 7.3:-71082041 MS.gene77076:CDS
CTACTGAAAAGCGTCCAGCA+TGG 0.659911 7.3:+71082977 None:intergenic
GGCCGAATTAAAGAACCGGT+TGG 0.664191 7.3:-71082259 MS.gene77076:CDS
TTACTCACAATTACACGAGG+AGG 0.673161 7.3:+71089443 None:intergenic
TACAAAGGATGATCTGCACA+AGG 0.676894 7.3:-71088192 MS.gene77076:intron
GTGAAAACAGAACGCAACGT+TGG 0.678738 7.3:-71090169 MS.gene77076:CDS
CAGACAAGAACAAGATGGAG+AGG 0.687695 7.3:-71083005 MS.gene77076:CDS
TATTTACTCACAATTACACG+AGG 0.705049 7.3:+71089440 None:intergenic
CATATCATGGACGTACCCTG+CGG 0.712045 7.3:-71083447 MS.gene77076:CDS
TCCAAACCTTATGAGCCATG+AGG 0.713107 7.3:+71087984 None:intergenic
TGATATTCTGATGTTCCCGG+AGG 0.735991 7.3:+71084671 None:intergenic
TGAAGCGCGTTATTCTGACG+GGG 0.739162 7.3:-71082825 MS.gene77076:intron
AAACAGAATAGAGAACTCAG+TGG 0.757960 7.3:-71089476 MS.gene77076:CDS
CATGAGAGTAGATCACCATG+AGG 0.766830 7.3:+71088650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.3 gene 71081969 71090374 71081969 ID=MS.gene77076
chr7.3 mRNA 71081969 71090374 71081969 ID=MS.gene77076.t1;Parent=MS.gene77076
chr7.3 exon 71090299 71090374 71090299 ID=MS.gene77076.t1.exon1;Parent=MS.gene77076.t1
chr7.3 CDS 71090299 71090374 71090299 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71090129 71090208 71090129 ID=MS.gene77076.t1.exon2;Parent=MS.gene77076.t1
chr7.3 CDS 71090129 71090208 71090129 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71089451 71089528 71089451 ID=MS.gene77076.t1.exon3;Parent=MS.gene77076.t1
chr7.3 CDS 71089451 71089528 71089451 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71088981 71089124 71088981 ID=MS.gene77076.t1.exon4;Parent=MS.gene77076.t1
chr7.3 CDS 71088981 71089124 71088981 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71088634 71088684 71088634 ID=MS.gene77076.t1.exon5;Parent=MS.gene77076.t1
chr7.3 CDS 71088634 71088684 71088634 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71088396 71088536 71088396 ID=MS.gene77076.t1.exon6;Parent=MS.gene77076.t1
chr7.3 CDS 71088396 71088536 71088396 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71088193 71088315 71088193 ID=MS.gene77076.t1.exon7;Parent=MS.gene77076.t1
chr7.3 CDS 71088193 71088315 71088193 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71087991 71088068 71087991 ID=MS.gene77076.t1.exon8;Parent=MS.gene77076.t1
chr7.3 CDS 71087991 71088068 71087991 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71087655 71087750 71087655 ID=MS.gene77076.t1.exon9;Parent=MS.gene77076.t1
chr7.3 CDS 71087655 71087750 71087655 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71087460 71087582 71087460 ID=MS.gene77076.t1.exon10;Parent=MS.gene77076.t1
chr7.3 CDS 71087460 71087582 71087460 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71085009 71085077 71085009 ID=MS.gene77076.t1.exon11;Parent=MS.gene77076.t1
chr7.3 CDS 71085009 71085077 71085009 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71084670 71084768 71084670 ID=MS.gene77076.t1.exon12;Parent=MS.gene77076.t1
chr7.3 CDS 71084670 71084768 71084670 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71084160 71084255 71084160 ID=MS.gene77076.t1.exon13;Parent=MS.gene77076.t1
chr7.3 CDS 71084160 71084255 71084160 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71083992 71084067 71083992 ID=MS.gene77076.t1.exon14;Parent=MS.gene77076.t1
chr7.3 CDS 71083992 71084067 71083992 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71083690 71083793 71083690 ID=MS.gene77076.t1.exon15;Parent=MS.gene77076.t1
chr7.3 CDS 71083690 71083793 71083690 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71083351 71083610 71083351 ID=MS.gene77076.t1.exon16;Parent=MS.gene77076.t1
chr7.3 CDS 71083351 71083610 71083351 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71083140 71083226 71083140 ID=MS.gene77076.t1.exon17;Parent=MS.gene77076.t1
chr7.3 CDS 71083140 71083226 71083140 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71082826 71083056 71082826 ID=MS.gene77076.t1.exon18;Parent=MS.gene77076.t1
chr7.3 CDS 71082826 71083056 71082826 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71082443 71082521 71082443 ID=MS.gene77076.t1.exon19;Parent=MS.gene77076.t1
chr7.3 CDS 71082443 71082521 71082443 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71082251 71082314 71082251 ID=MS.gene77076.t1.exon20;Parent=MS.gene77076.t1
chr7.3 CDS 71082251 71082314 71082251 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
chr7.3 exon 71081969 71082126 71081969 ID=MS.gene77076.t1.exon21;Parent=MS.gene77076.t1
chr7.3 CDS 71081969 71082126 71081969 ID=cds.MS.gene77076.t1;Parent=MS.gene77076.t1
Gene Sequence

>MS.gene77076

ATGGGAATGAGTGGGACACGAGTGCAATTGAATAAACCTCACAAGTCTCGTTTCTCTTCTAAATCTTCTCTCAAAGGTTTGTCACGTCATGATTTTGATCATCACGTATAATCTTCATGTATTGCTTTGATTAACGGTTTGTTTGCTTTTTTATTGTGTTGTTTAGATAGAATAATTCCAGTTGTGAAAACAGAACGCAACGTTGGTAAAGGAGCACGTGCTGCTCGATTACAGAGAAATAAAATGGTACCTATTATATGTTCTTGTTTGTGCTTTACTCTATACTTACTTGGAGCACCGACACTTCTGATCAAAGGTGTGTTTTTTGTCCGACACAGACACATATAATTACATTCAATTATTCAAAATAGTACCGGTGTCGAAGTGCAGTGTTGGGCTGCTTCATAGGTGCTTTATCGTTGTATTTAGAAAGAAATAAAAAAAAAAAAAAAAACACAATTTAGTACCAAGTATATGAAAACCATCAAAAAATTTGTCGAGCCTTGCGGCAAAACTCACATGCCAAGTGTTTGAGAAGTGGAAAATTGTGGGCTCGAATCTGCGGCCAACTTTTCAAATGTCCCTGCAGTATTCTGAGTAGGATACTGAAAATCGTCAATTTAGTCCTTGTAGTTATGAAAATTTGTAATGTCAGTCCCTAACATCTAAATGACGGAGAAAAAATTGATGATTTTTATAACTTTAGGAACTAATTATTATATTTGTATACTTCAGAGACTAAATTAGATAGAGAGTGATACATCAAAGATTAATTGATGGTTTATTTATTCTTGTAGTTACTATTTCCAGAGTTGTCTCTGATCGATCTGTGAATTTACAATACAGATACGTGAACAGAAAAGGGCTGCAGTATTGAAACAGAATAGAGAACTCAGTGGCTCAACAACTCCTCCTCGTGTAATTGTGAGTAAATATTAATTCCGGCTGTCTTTTGTTAAATTGAATTTCACACTTATTTTCAGTATGATACCGTGTGTCACACGTCACTCGTTGGTTTTTACGATTTTTCACATAATACTAACTGTGCAGCATAGGTATTAAATATTCACAAGTACTTCAATTCTTTTTACATAATTGCCTATTTTCAAACATGGTTAAATCTTATTTGTGTCAAATAAATGAATATCCTTTCCAATATACATTCAAATGAAAGTATTTCCTGCAAAATTAGAGGTCAAGTTATTAAGTACAAAAGTCGACATTGTTGACATTTTAATGTGCCGTCACAGGTTCTCTTTGCTCTATGTGCTTCTGTGGATTTAGAATCACTTGCAAATGAGCTCTCATCATTATTATCTAAAGATTCTTCTACAGTACTCCCAGGATTTGCAATACCAACAGTTGCTTCTTCCGAATATAAGATTAGAATATCGGTACATTCATCTGATTGATTACTGCCTTATTATTATCTGATAATTAGAAAATTTATTGTACATGAATTGAGTTGTTGATAGCTAAAATAGACTTTCGAATTTAATATTTAATGATATGAAGTCATGAATTTAATAATTTATTATTCATTCTCATATATCAGAATATTGACCTGCATTTGGGAAACTAGGACGACAATTGTAATTTAAACATGTTTTCGAATATATAATGATCCTTTTCCCAAGCAAGTTGTTATGGATGGGATTTGACATGAACTTTGTATGCTACATGGCTACAGGTACTAAAAGCACCTCATGGTGATCTACTCTCATGCATGGAAATGGTCAAGGTATCAATTTTTACATTTGCGTTGCCTCCATGACTTCTGTTCATGGCTGTGGAATGGGTTTTGATTACTGCTGTGTTGGACACTGTTTATGTTGTAGGTTGCTGATCTACTGGTTTTTGTGGCCTCGGCAAAGTCTTCATGTGAAGAAACTGCTTCTCAGTACATTGATTCGTTTGGAAATCAATGCCTTTCTGTATTTAGGTCTCTTGGTTTACCAAGCACTGTTCTCTTCGTTCGTGTAAGTTAGGATGGATCATATTTTTGGAAGCTATGAATTGAGTTACACGGACATCTAGTCATTTCATTTTATGCTTTTAGGATCTTCCCACTGAGCTAAAACAAAGAAATGAACTAAAGAAGTCGTGCTCATCAAGTCTTGCCTCAGAATTTCCTGAGGATTGTAAATGTTATCCAGCTGATACAAAGGATGATCTGCACAAGGTAATTATTTCAACACTTGTTGGCTGAAAAATTTATTTGTAACCGTTAAGCTTATCATCTATTGTATATCCAGTTTGATAGTTTAACGTTAAGCCTTTCACTCCTTCCGTCTTCATAATTGCAGTTCTTGTGGCTCTTTAAAGAGCAATGGCTTAAAGTTCCTCATTGGAGAACTCAACGATCTTACCTCATGGCTCATAAGGTTTGGATTCAACAGCCTAGTCATATTGGTTTCGATCATGTTTTCTAAGATAATTTATTAGTATATGTAGTTTTGTAGCACTAAACAATCCACAATATCATAACTTTTTTAACATGGGTGAATAAATAATTTCTGACCATATTATATTCACCTTTTCTGTAAAGCCCTTATGCAATTTGATGAAGATGTTGATTGATGATATTGCATTGAGCTTATCATGTTTTCAACCGTAAATTTCAGGTCGATATTGATGATTGTACTGATGGTAATTCTGACAAATGCACCCTTCTTCTCGCTGGTTATCTTTGTGCTCACAGCCTCTCTGTGAATCAAATGGTAATGAGCAAGATCATATAATAGGTTACAGTTTCATTAAATTGTTGGTAGTCTCACATTTTGTTTGTTCAGGTTCATGTATCAGGTGCTGGAGATTTCCGACTGTGTAAAGTTGAAGTTCTCGAAGATCCCCTTCTTTTGAACTCAAGTAAAAATCAAGACCTAATGGGTTCAGATGAGATGCACGATGCGAAGGTATAGAGTTTTACAGAATTTTTTTAGCCATTAAGGGTTTCTACATATATTATTTAATTATATGACAGTAAGGTTAAAACATATAATATGCATATTTATAGGGTCGGCTATGAGCCTGTGCAAAGAGGCTTGTAGCACGCAACCTCGAAGTTGTGAGGACCCCAAATTCTCAAAAAATCATGAGTTTAAACGACCTTGCATCGAAGCTGTGTTTTCTATCAGTTTTGAGGGGCCCAAATTCATAACTGTATTGGGAAATGTTCAATGCTTATAAGAAGGTTTTTAATTCTGTCAACTTATTCATAGACATCTTCCCTGTCTTTTGCTTGCCAAACTATCTGACGTGGGACTTACATCTTTGTTTAGTATGATGTTCAATGCAAACTTGTGTAGCAACTGGTGAACAAGATTCCCCATTTGCGCCTTGAACTTCTAGAGTTGGCCTTGGGACTTTCACTTTCATATTCCGAGTTTGACGACCAACTACTAGGGGAACAGTTACGTTAGCTGCCTGATTCATTTGATTCTCTAGTGTTGACTTACAAACAAAATCCTCCACACCAACAACACAAGAATCCTTCATAAGAAAACCCACTTGAGGATCTTGAAGCTTGGATAACTTCATGAAAGATTCAGAACCAAAGGCCATTGAACTTGCATTGAACTCATGATTAAGTTCTTTTGTAATTGTCATGTTGGTATTGAGTTGATTGAATAAAAGCAACATAAGTTCCACATCCCTTTGACAAATTAGCAGTTTTCATAGCCTCCAAATAAATTGATAAGAAGTTGTCTCCTTTGTGCCCTGTTGATACAGAAGAAAACTCCATGCACAGCCGCCAACGACTAAAGACTCAGAGTAAATCTTCTCAACATTCAACCGAGAGAAATTCTCAATCTTCCATGTGAATTTCTCAAAAATCTCAACACTTGTTTCTTGATGCTCTACTTCTACAAACTCAATAAGATTGACGCAGCCAGCTCCTAAGAACGGCCAGACTTTGAAAGAGAAAGAAGTCACACTTACTGAGTTTGTAGAAATGGAACATCAAGAAACAATTGTTGAAATAGTTGAGAAATTCACATGGAAGATTGAAAACTTCTCTCGCTTGAATGTTGAGAAGATTTACTCTGAGCCTTTCGTTGTTGGTGGCTATCCATGGAAGATTCTTCTGTATCCACACAGGGCACAAAGGAGACACCATCTTATCAATTTATTTGGTGGCTATGTGAACTGCCAATATGCCAAAGGGATGAAGTAGAGATGTGAAACTAAGTTTTTTTTATTCAATCAATTCAATACCAACATTACAATTTCGAAAGAACTTAACCATGATTTCAATGCACGTTCGATAGCTCAGGGCTTCAGGGCTCCAGATCTTTCATGAAGTTATCTAAGCTTCAAGATCCGCAAATGTGTTTTCTTGCGAAAGATGCTTGTATTGTTGGTACGGAGAATTTTCATTTTAAGTCAACACTTGAGAATCAAGTGGATCAAACAACTAACGTAACTGTTTCCCTATGAGGTGGAAGTCAAACTGGGCATATGAAAGTAGAAAGTCCAATGCCAAATCCAGAAGTTCAAGGCCAAAATGTGGAAACTCATTCACCAATTTCTACACAAGTTTGTAACTCCGTCATCAGACAGTTTGGCAATAAGTTGTGTAGAATGGACTTCAGAGTGTGCAAACAGAGATTAATGCACTTACAGTCCCAGTATTCAGAACCTTGTTATAAGTGTTGAACACTTCACAATACAGTTATTAATTTTTTTGTTTTTATAATAGTTTCTATCTCTGATTTGTGTTTTGAATTAGAAAATCTGATGTAAAACCTCATTTAAACTCGTCACTTTTTTAGAATTTTGGGCCCTCAAAATTTTGAGGCCTTGTGATATGGGTCTGTTTGTACAAGCACATAGCCGCCACCTGCCTAATACTATCAAGGACCGAAATTCATCTGCTGTAGAACACAATATCTCAGGAGTGTTAGGTGGGTTATTCCATAGCCTTTAAGAAAAAAGTAACACAAGTAGGAGACTCTTTTTATATACGTAAGGCCGTCTTTAACATTTTGGAGGTCCTGCTGCTCTAATCTAAAAGACGGCCTTGCATATTTAAGCATGTAAATAAAATCATGGAATCTGAATATTGATATAACACTTTTAATTGGTGAGGTCATTTTTTAGTCTATTAGAAAACACTGTCACCCTTCCCCCTTTTAAGTTTGATATAATGAAATTTTAAGTCTACTGTGCATGCAACATTTGGACATTGTGATCTTGCAAAATTTTTACCTTTTTAATCTCATACGTTCTTTAACTATTTGTCTATATCTACACTTTAATTTTTCCCTCTACTTTCCTGCTTTATAATGTTTTAGGTCATTCATTCTCTGGTTCCAGATCGTCAAAATCTGGAAGATATAGTTGTCAAGAATGCTGGAGAGCAGGTAGATAAGGAGATCCTATTTCTAATTCTGCCATAACTATTTGTTAATCGTAAGATTTGATTTTTGAGATTTTCTACTACTAATTTCTGAATTGACATCTTTTAATTGAATACAAGAGTACTCCTTAATTTTACTTTTTGCACTACGACAAAATGATAGGATGAGCCGTTACTGCCTATCAAATTAGTTGCAAGATTTTTTTTCCTTAGCATTTTGAGTGATATCATTCCCTATTACCAGACACGCCCAAATGAGGCTGATGGAACAAAGGCAGATGAAGATCATGAGCAAAAGAAAACAAGAAAGAGAAAGCTTCCTCCGGGAACATCAGAATATCAGGTTTGAGCTTGTTTTCATGATACAGTTATTTCTGAAAGATTTTTGTTACCCTTATTATTTTGATTATTTTTCCTGATGTTTTCTGTCATATTTATGCTTACCAAGTAGGTATTACCTACGTCTCATATTATATGTCTTTTTAAGATAATGCACATGTCCTAAGAAAATGATTAGTTATTTTGAATTCAATAGTAAAATAGTGTGTATAGTGTATATAGTCTAAACTACACATATTAGTTAGAGGTTGAAAGTAGCAGTAGTAGTTGTAGTGGTGAAATAGTTCGGTGGACTACTTAATTAAATGACAAATAACATGATACAAAAATGTTTCAAATGAAACATTTATAATGAGACATAGCCACGGAGGTAGTATCTAATATGGAACAAATTTTTGGCCTTTATTTTGATTTTTAGGCTGCATGGGATGAATTCGATGAAGAGGAGTCAGATTATGGCATTGAAGATGATGATGGAGATTCTGAGGAAGAAAGTGACAAAGTTTCAATGATGGTGAGTTTGTAACAACTTAATACTGAAGTGTTTTGGCATCATTGGATAGTCTCCGGTCATTAATGCATGATTACTTTTTTGTTGGATTTCAGGAAGTAGATGACATGACTGAAGAGAAGATACAAGATGAACTTAAGGCAATTAAAGAAGCTCATGCTGCAGATGAGGGTAATTTTTATTATTTTATGTTCTTATGTTTGTCTGTGTGTCACATTTGCTTTTTATTAGAAAAAACCATAAAGTTGAAGAGTTTTGCGTAGGTGACATGGAATGTACCTGTCTGTGGATGATTTGCAATTGTATCGCATTGATGATATAATGATTTTAAATAATGATTTGTTGCTTGATATATGTTTTTTGTTGTAGAGTTTCCCGATGAAGTGGATACGCCATTGGATGTCCCAGCTAGGACGCGGTTTGCAAAATATAAGGGACTCAAGTCTATTCGGAATTCTATATGGGATCCTATGGTATTTTATCACTTCGTTATTCTTTAATTAGTCGTTTCTATTAACAGCAAAAAATTCTGACATTTCTAATTTTTATCAGGAATCTCTACCCCAAGATTATCCCAAAAATAACAATTATCATAGTTTCAAAAGAACACAAAAACAGGTTCTTGCTAAAGCTTTAGAAATGGAACGAGAAAACCGAGAAGACTGTGTTCCAGTTGGCTCATACATTAGGCTGCATATCATGGACGTACCCTGCGGTGTTGCTTCAAAGTTATGTATACTTGCAAAGACACTACCGGTTACAGCATGTGGTCTGCTGGAACACGAAAACGAAGAATCTGTCCTTCATTTCAGGTATAGTTATGCTTTATCATTTTACTATTTCATTTTTAGTGCTTTTCATACTGTACTAATTAAAAAGTGATCCTCTGAATATTCAAAAGACGTGTCATGAAATAAAGTCGCTCATGTTTTGTAGTGTGAAGAAGCACGAAATGTATCACGCGCCCATCAAATCCAAGGAAGAGTTAATATTTCATGTTGGGTTTCGGCAATTTGTTTGCAGGTATGGTATTCAATTTAAACAACCATGACATAATAAAACTCGTTAGGAAAAAAAAGTATAAGAAATGGTGACAAATTTTGCAGGCCGATTTTCTCATGCGAATTCAAAAACGCAGACAAGAACAAGATGGAGAGGTTTCTCCATGCTGGACGCTTTTCAGTAGGTTCAGTTTATGCACCTATTTCATTTTCTCCCTTTCCTACAATAATCCTCAAGAGAGCTGGAGAGGATGCTGCACCAGAAGTAGCTGCTTTGGGCTCACTGAAAACTGTTGATCGAGATAGGATAATCCTGAAGCGCGTTATTCTGACGGGGTATGTTTGTTGATGTTTGCCATAACCTATCAATTAAAAGCTGTTAGATATGAAAATATCTCATCATATATCAAAATTGTTTTATAATGTCTTAGGTTATCTATTTGCATTAGTTTCTATATTTCAATATTTCTACATTGTTATCGCCTATTACTTCAACAAAAATCCTTGCACGTTACTTTTTTGTCGACGCCTTTTCAATACTGATTCTGGCAACTTTGTAGTTATTGTCATTTAAGAGTTGGGACAATCCCCAATAAATTTGTTTTACTAATTAGAAGCTTTTAAACATTTTGTGGTGCAGTTACGCCCAACGAGTGTCGAAACGTAAAGCTTCTGTGAAACACATGTTTTATAATCCAGAGGATGTTAAATTCTTTAAGGTAAGAAAAAATGGTACCCAGTTTCTGAAATCATTACTTTCTTTCCATGATGAATTGGATAGAATTGACATTAATTTGTTCTCAGTTTTCACACTAACGAGATTTCTTAATGTGGGTGGTAATTGCAGCCTGTAGAGATTTATACCAAACGCAGTATTCGTGGCCGAATTAAAGAACCGGTTGGTACACACGGTATGTTATGTTAGAAATCTTTACCTTGTATCTTTCAAGGTCATTTATTTATATTTCAGATTATATAGCATTCATATAAATCTATTATATCCTGATGGTATTATTCTGTTTATTGTTTGTTCAGGAGCAATGAAGTGCCTTCTCAACGGAGTTCTTGAACAGAGTGATACTGTATGCATGAATTTATACAAACGTGCATTCCCCAAGAGGCCAAACCATCTTTTTCCTTTAAGCTCTGCTTCAGATTCACTTCCATGTATGGAGGATCAAAATGCAGTTTAG
Protein sequence

>MS.gene77076.t1

MGMSGTRVQLNKPHKSRFSSKSSLKDRIIPVVKTERNVGKGARAARLQRNKMIREQKRAAVLKQNRELSGSTTPPRVIVLFALCASVDLESLANELSSLLSKDSSTVLPGFAIPTVASSEYKIRISVLKAPHGDLLSCMEMVKVADLLVFVASAKSSCEETASQYIDSFGNQCLSVFRSLGLPSTVLFVRDLPTELKQRNELKKSCSSSLASEFPEDCKCYPADTKDDLHKFLWLFKEQWLKVPHWRTQRSYLMAHKVDIDDCTDGNSDKCTLLLAGYLCAHSLSVNQMVHVSGAGDFRLCKVEVLEDPLLLNSSKNQDLMGSDEMHDAKVIHSLVPDRQNLEDIVVKNAGEQTRPNEADGTKADEDHEQKKTRKRKLPPGTSEYQAAWDEFDEEESDYGIEDDDGDSEEESDKVSMMEVDDMTEEKIQDELKAIKEAHAADEEFPDEVDTPLDVPARTRFAKYKGLKSIRNSIWDPMESLPQDYPKNNNYHSFKRTQKQVLAKALEMERENREDCVPVGSYIRLHIMDVPCGVASKLCILAKTLPVTACGLLEHENEESVLHFSVKKHEMYHAPIKSKEELIFHVGFRQFVCRPIFSCEFKNADKNKMERFLHAGRFSVGSVYAPISFSPFPTIILKRAGEDAAPEVAALGSLKTVDRDRIILKRVILTGYAQRVSKRKASVKHMFYNPEDVKFFKPVEIYTKRSIRGRIKEPVGTHGAMKCLLNGVLEQSDTVCMNLYKRAFPKRPNHLFPLSSASDSLPCMEDQNAV