AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026144.t1 MTR_1g040175 80.043 461 61 3 1 433 1 458 0.0 753
MS.gene026144.t1 MTR_1g040320 81.128 461 23 3 1 433 1 425 0.0 738
MS.gene026144.t1 MTR_1g040215 71.150 461 105 2 1 433 1 461 0.0 681
MS.gene026144.t1 MTR_1g040195 67.706 449 95 3 1 423 1 425 0.0 615
MS.gene026144.t1 MTR_1g040315 67.376 423 121 4 1 423 1 406 0.0 565
MS.gene026144.t1 MTR_7g114560 53.468 447 174 6 5 421 6 448 2.38e-170 486
MS.gene026144.t1 MTR_7g114550 51.868 455 185 6 2 426 3 453 7.18e-170 484
MS.gene026144.t1 MTR_1g081660 54.795 438 168 5 4 413 13 448 6.29e-169 482
MS.gene026144.t1 MTR_5g076700 52.183 458 184 6 5 431 6 459 1.01e-168 481
MS.gene026144.t1 MTR_7g114520 52.203 454 171 7 5 421 7 451 1.09e-166 476
MS.gene026144.t1 MTR_7g114470 51.957 460 183 7 5 431 7 461 2.31e-165 473
MS.gene026144.t1 MTR_7g114490 51.508 431 176 5 5 405 6 433 4.79e-156 451
MS.gene026144.t1 MTR_0042s0190 67.340 297 69 2 165 433 1 297 5.50e-141 405
MS.gene026144.t1 MTR_0042s0190 65.948 232 51 2 230 433 1 232 6.09e-101 300
MS.gene026144.t1 MTR_7g114510 46.154 312 123 6 146 421 7 309 2.64e-87 269
MS.gene026144.t1 MTR_0042s0230 84.354 147 23 0 1 147 1 147 3.90e-86 259
MS.gene026144.t1 MTR_0502s0010 53.306 242 78 4 100 310 1 238 2.92e-82 253
MS.gene026144.t1 MTR_3g035000 65.482 197 64 3 199 392 43 238 3.03e-79 248
MS.gene026144.t1 MTR_8g039950 79.688 128 26 0 1 128 1 128 5.26e-71 220
MS.gene026144.t1 MTR_3g098980 31.542 428 249 10 9 405 14 428 2.29e-67 221
MS.gene026144.t1 MTR_7g114420 42.963 270 128 6 165 421 1 257 6.08e-66 212
MS.gene026144.t1 MTR_7g114500 59.333 150 61 0 2 151 3 152 7.93e-64 202
MS.gene026144.t1 MTR_8g096530 30.876 434 255 11 1 403 1 420 2.28e-63 211
MS.gene026144.t1 MTR_7g027175 30.969 423 247 11 13 403 20 429 4.12e-62 207
MS.gene026144.t1 MTR_3g051790 62.329 146 55 0 2 147 6 151 2.03e-59 192
MS.gene026144.t1 MTR_1g053315 29.478 441 264 13 17 413 26 463 7.28e-47 167
MS.gene026144.t1 MTR_3g112180 31.088 386 217 12 17 364 20 394 2.61e-46 166
MS.gene026144.t1 MTR_4g007540 28.941 425 251 12 9 393 3 416 2.23e-45 163
MS.gene026144.t1 MTR_4g055170 27.556 450 282 9 3 410 6 453 3.57e-44 160
MS.gene026144.t1 MTR_2g105330 27.107 439 269 12 9 409 3 428 1.96e-41 152
MS.gene026144.t1 MTR_8g075610 27.230 426 239 10 16 393 1 403 3.67e-41 151
MS.gene026144.t1 MTR_2g064495 27.107 439 269 12 9 409 3 428 6.76e-41 151
MS.gene026144.t1 MTR_7g110610 30.151 398 218 13 5 384 1 356 2.04e-40 149
MS.gene026144.t1 MTR_7g084910 27.559 381 240 10 8 364 16 384 8.60e-37 140
MS.gene026144.t1 MTR_3g071320 27.342 395 244 12 9 364 5 395 5.94e-34 132
MS.gene026144.t1 MTR_7g085990 26.078 464 264 16 9 407 2 451 1.05e-33 132
MS.gene026144.t1 MTR_7g084940 26.087 391 247 9 8 364 15 397 1.47e-33 131
MS.gene026144.t1 MTR_4g088765 79.452 73 15 0 1 73 1 73 1.48e-33 122
MS.gene026144.t1 MTR_3g465990 26.338 467 274 15 3 412 4 457 1.29e-31 125
MS.gene026144.t1 MTR_2g015430 28.165 387 239 11 9 360 2 384 3.76e-31 124
MS.gene026144.t1 MTR_7g056087 26.351 444 254 16 16 407 11 433 3.83e-31 124
MS.gene026144.t1 MTR_3g465930 29.967 307 184 9 7 293 8 303 4.41e-31 124
MS.gene026144.t1 MTR_7g086000 27.317 410 216 15 17 358 12 407 4.98e-31 124
MS.gene026144.t1 MTR_7g085930 25.829 422 242 14 9 365 6 421 9.21e-31 123
MS.gene026144.t1 MTR_3g465980 26.681 461 268 16 9 412 10 457 1.25e-29 120
MS.gene026144.t1 MTR_3g465910 29.412 306 191 9 7 296 8 304 4.02e-29 119
MS.gene026144.t1 MTR_6g053810 23.605 466 292 12 3 412 5 462 7.12e-29 118
MS.gene026144.t1 MTR_3g465880 26.513 347 220 10 7 328 8 344 2.44e-26 110
MS.gene026144.t1 MTR_3g465950 28.146 302 196 9 7 293 8 303 1.42e-25 108
MS.gene026144.t1 MTR_3g465870 28.620 297 185 10 5 283 8 295 1.49e-25 108
MS.gene026144.t1 MTR_3g026950 23.833 407 245 10 17 365 10 409 3.69e-25 107
MS.gene026144.t1 MTR_4g127650 24.697 413 249 12 8 365 9 414 5.73e-25 107
MS.gene026144.t1 MTR_4g013050 23.556 450 220 15 17 403 12 400 2.87e-23 101
MS.gene026144.t1 MTR_4g097090 24.444 450 271 14 1 393 1 438 3.51e-23 101
MS.gene026144.t1 MTR_5g090830 23.656 465 247 16 7 400 8 435 1.92e-22 99.8
MS.gene026144.t1 MTR_5g090810 23.043 460 256 15 7 400 8 435 1.81e-21 96.7
MS.gene026144.t1 MTR_3g049150 25.000 408 231 13 17 363 10 403 2.15e-21 96.3
MS.gene026144.t1 MTR_5g090880 24.548 387 209 13 61 387 64 427 1.22e-20 94.4
MS.gene026144.t1 MTR_5g084870 22.556 399 227 14 15 361 9 377 4.62e-20 92.4
MS.gene026144.t1 MTR_3g450710 23.832 428 220 16 64 419 65 458 7.46e-20 92.0
MS.gene026144.t1 MTR_3g450690 24.242 396 202 14 64 395 72 433 1.19e-18 88.2
MS.gene026144.t1 MTR_3g086020 23.350 394 222 16 15 363 11 369 1.84e-18 87.4
MS.gene026144.t1 MTR_8g079970 25.817 306 179 10 15 299 9 287 1.98e-18 87.4
MS.gene026144.t1 MTR_3g070170 24.766 428 224 15 53 405 55 459 3.91e-18 86.7
MS.gene026144.t1 MTR_7g114417 67.308 52 16 1 89 139 29 80 1.90e-17 77.4
MS.gene026144.t1 MTR_8g079950 33.929 168 93 6 15 175 9 165 3.91e-17 83.6
MS.gene026144.t1 MTR_5g084850 24.427 262 172 8 15 269 9 251 1.14e-16 82.0
MS.gene026144.t1 MTR_4g094842 25.266 376 221 14 48 372 46 412 1.53e-16 81.6
MS.gene026144.t1 MTR_4g094828 23.881 402 248 13 51 405 49 439 2.47e-16 81.3
MS.gene026144.t1 MTR_0190s0020 24.198 405 240 18 48 405 47 431 7.24e-15 76.6
MS.gene026144.t1 MTR_3g051820 47.312 93 45 3 340 431 1 90 7.38e-15 70.1
MS.gene026144.t1 MTR_8g079710 24.000 350 209 12 59 360 54 394 7.71e-15 76.6
MS.gene026144.t1 MTR_3g101930 27.757 263 161 12 64 306 70 323 9.46e-15 76.3
MS.gene026144.t1 MTR_4g022290 22.222 396 243 14 59 405 55 434 2.58e-14 75.1
MS.gene026144.t1 MTR_5g084900 22.054 331 192 12 61 361 6 300 9.21e-14 72.8
MS.gene026144.t1 MTR_5g026320 26.408 284 177 12 22 293 33 296 2.04e-13 72.4
MS.gene026144.t1 MTR_3g101960 27.490 251 163 10 61 299 65 308 6.77e-13 70.5
MS.gene026144.t1 MTR_3g101900 24.351 308 183 11 61 350 63 338 8.08e-13 70.5
MS.gene026144.t1 MTR_4g022800 24.727 275 179 9 23 283 25 285 1.05e-12 70.1
MS.gene026144.t1 MTR_7g026860 27.092 251 161 11 48 283 42 285 3.99e-12 68.2
MS.gene026144.t1 MTR_2g007880 24.199 281 176 12 6 274 14 269 4.19e-12 68.2
MS.gene026144.t1 MTR_4g094832 27.315 216 138 8 94 300 85 290 5.04e-12 67.8
MS.gene026144.t1 MTR_6g016815 31.250 144 86 6 48 184 56 193 5.21e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026144.t1 AT5G17540 51.392 467 187 10 1 433 1 461 1.03e-157 454
MS.gene026144.t1 AT3G03480 49.550 444 182 10 5 414 15 450 1.28e-138 405
MS.gene026144.t1 AT5G41040 33.005 406 224 11 7 377 15 407 9.15e-69 225
MS.gene026144.t1 AT5G41040 33.005 406 224 11 7 377 31 423 1.02e-68 225
MS.gene026144.t1 AT5G63560 30.734 436 251 11 1 403 1 418 7.72e-68 222
MS.gene026144.t1 AT3G48720 29.032 434 265 10 1 403 1 422 2.33e-65 216
MS.gene026144.t1 AT3G62160 30.597 402 218 11 5 367 1 380 2.11e-46 166
MS.gene026144.t1 AT5G48930 28.315 445 260 13 8 409 2 430 1.38e-43 158
MS.gene026144.t1 AT1G27620 27.556 450 272 12 10 423 10 441 1.18e-42 156
MS.gene026144.t1 AT1G03390 30.435 391 226 13 14 364 27 411 1.13e-41 154
MS.gene026144.t1 AT1G28680 27.009 448 277 15 13 417 8 448 2.48e-39 147
MS.gene026144.t1 AT5G57840 27.361 413 238 14 8 370 2 402 1.46e-37 142
MS.gene026144.t1 AT2G40230 28.861 395 232 13 9 363 5 390 5.28e-35 135
MS.gene026144.t1 AT1G28680 27.869 366 223 12 13 341 8 369 6.35e-35 134
MS.gene026144.t1 AT1G65450 27.632 456 259 15 7 413 16 449 7.75e-35 134
MS.gene026144.t1 AT2G19070 25.735 408 244 12 12 365 7 409 8.86e-33 129
MS.gene026144.t1 AT3G47170 27.914 326 196 9 4 299 7 323 1.87e-31 125
MS.gene026144.t1 AT2G23510 27.195 467 264 18 1 412 5 450 2.40e-31 125
MS.gene026144.t1 AT5G07080 26.161 409 226 13 9 360 14 403 2.84e-30 122
MS.gene026144.t1 AT2G25150 26.374 455 273 17 9 414 13 454 7.23e-29 118
MS.gene026144.t1 AT1G78990 26.684 386 211 13 62 404 65 421 8.15e-29 118
MS.gene026144.t1 AT1G32910 27.228 404 228 14 62 414 63 451 1.48e-27 114
MS.gene026144.t1 AT1G31490 26.536 407 226 18 6 362 3 386 4.61e-24 104
MS.gene026144.t1 AT1G24420 26.835 395 216 13 15 361 11 380 1.03e-23 103
MS.gene026144.t1 AT1G24420 26.835 395 216 13 15 361 11 380 1.03e-23 103
MS.gene026144.t1 AT3G26040 25.063 395 230 15 15 363 9 383 2.84e-21 95.9
MS.gene026144.t1 AT4G31910 26.276 392 222 14 25 357 24 407 4.28e-21 95.5
MS.gene026144.t1 AT5G47980 25.652 460 258 19 15 420 11 440 8.12e-20 91.7
MS.gene026144.t1 AT5G16410 23.864 352 200 13 62 363 89 422 1.48e-17 85.1
MS.gene026144.t1 AT1G24430 26.923 260 161 8 15 268 11 247 2.30e-17 84.3
MS.gene026144.t1 AT5G07850 24.444 360 217 12 64 375 70 422 1.51e-15 79.0
MS.gene026144.t1 AT5G38130 25.373 402 224 18 23 372 48 425 3.44e-15 77.8
MS.gene026144.t1 AT3G50280 23.611 360 213 14 64 372 59 407 8.45e-15 76.6
MS.gene026144.t1 AT3G30280 25.347 288 177 12 7 280 4 267 8.68e-15 76.3
MS.gene026144.t1 AT5G67160 23.596 445 273 16 13 410 2 426 4.09e-13 71.2
MS.gene026144.t1 AT5G47950 24.910 277 171 10 7 274 4 252 1.49e-12 69.3
MS.gene026144.t1 AT1G65450 33.333 147 93 4 7 151 16 159 2.35e-12 65.5
MS.gene026144.t1 AT3G23840 26.214 206 114 6 80 270 81 263 3.01e-12 68.6

Find 98 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGCAATGATTTCAAAAT+TGG 0.167630 1.2:+24699544 None:intergenic
GGGTCTTTCCCTGCCATATT+TGG 0.199762 1.2:+24701449 None:intergenic
TCTCCTTTATCATTTGTAAA+AGG 0.217002 1.2:+24699093 None:intergenic
TTTGATGGAGTTATCAATTC+AGG 0.239710 1.2:+24701566 None:intergenic
GATATTACTCTTAAACAATT+TGG 0.242406 1.2:-24701287 MS.gene026144:CDS
GTTCCTTTGTTGTCGGGTTC+TGG 0.275311 1.2:+24699671 None:intergenic
TCATCCTCCATTTCCTTGCT+TGG 0.281673 1.2:-24701255 MS.gene026144:CDS
CACTTTGAATACTAGAGATT+TGG 0.294557 1.2:+24701601 None:intergenic
TTATAACCATGATCCAAATA+TGG 0.301048 1.2:-24701462 MS.gene026144:CDS
TATAATCCAGCAGTAGTTAA+AGG 0.314645 1.2:+24699240 None:intergenic
ATCCATTTGCAGGTAGGTTA+AGG 0.320236 1.2:-24701379 MS.gene026144:CDS
GAACTTCTTTATGATGTTCC+TGG 0.323967 1.2:-24701230 MS.gene026144:CDS
AAATCATTGCTGCATACTTT+TGG 0.324455 1.2:-24699535 MS.gene026144:CDS
CGATGAGTACATGTTACTTT+TGG 0.324557 1.2:+24699704 None:intergenic
GGATTTAGAGATGTTGATTT+TGG 0.336184 1.2:-24699192 MS.gene026144:CDS
GCATTCAGTGGCCGATTTCA+TGG 0.342688 1.2:-24699280 MS.gene026144:CDS
GGAGGGGATGGTGCTCTTCC+TGG 0.355512 1.2:-24699135 MS.gene026144:CDS
GTTCACCGCACTTTCTTCAT+TGG 0.359527 1.2:-24699626 MS.gene026144:CDS
TTTGTCAGAATCTAGTTGTA+AGG 0.385494 1.2:+24699496 None:intergenic
CGTCCTCCTTTAACTACTGC+TGG 0.389428 1.2:-24699246 MS.gene026144:CDS
CTTCAATTCTTGATTGCCTT+AGG 0.397733 1.2:-24699803 MS.gene026144:CDS
TCCATTTGCAGGTAGGTTAA+GGG 0.408126 1.2:-24701378 MS.gene026144:CDS
ACAAATGATAAAGGAGAGAA+AGG 0.408216 1.2:-24699087 MS.gene026144:CDS
AACATGGTTCCTTTGTTGTC+GGG 0.413970 1.2:+24699665 None:intergenic
AGTTCATCCAAGCAAGGAAA+TGG 0.414422 1.2:+24701248 None:intergenic
ACCCTTGATAACCATGAAAT+CGG 0.418991 1.2:+24699269 None:intergenic
TCCCTGCCATATTTGGATCA+TGG 0.420285 1.2:+24701456 None:intergenic
GCGGTGAACCATGTCGTCTA+AGG 0.420378 1.2:+24699640 None:intergenic
CTTGCCAACGTAACGAATCT+TGG 0.424099 1.2:+24701501 None:intergenic
AGGTCCTAGTAGGAAACTTA+TGG 0.427564 1.2:-24701354 MS.gene026144:CDS
CTCCACACCGGTGAGATTGA+AGG 0.429413 1.2:+24699755 None:intergenic
GCGAATTGCAGCTACCTTAG+TGG 0.432300 1.2:+24699601 None:intergenic
TCCCTTAACCTACCTGCAAA+TGG 0.437418 1.2:+24701377 None:intergenic
CAACATTAACCAGCCTACTA+AGG 0.446515 1.2:-24698989 MS.gene026144:CDS
GGCCGATTTCATGGTTATCA+AGG 0.450203 1.2:-24699271 MS.gene026144:CDS
AAAATCAACATCTCTAAATC+CGG 0.451883 1.2:+24699194 None:intergenic
CACTCCATTGCTACTTATCC+AGG 0.458160 1.2:-24701189 MS.gene026144:intron
GGAGAGGTTGGTTAAAGAGT+TGG 0.469038 1.2:-24699028 MS.gene026144:CDS
AACCATGATCCAAATATGGC+AGG 0.473833 1.2:-24701458 MS.gene026144:CDS
CTGATGCATCACTCATTGTA+TGG 0.478329 1.2:+24699831 None:intergenic
AAACATGGTTCCTTTGTTGT+CGG 0.483476 1.2:+24699664 None:intergenic
CCATTGTGATCACCCTTAGT+AGG 0.484592 1.2:+24698976 None:intergenic
AAAACTTATTGAAAATCCAT+TGG 0.488114 1.2:-24699358 MS.gene026144:CDS
AAACTTATGGTTGATTGTAA+TGG 0.490791 1.2:-24701341 MS.gene026144:CDS
GCACCATCCCCTCCTTTAGC+TGG 0.492161 1.2:+24699144 None:intergenic
TGTGATCACCCTTAGTAGGC+TGG 0.505166 1.2:+24698980 None:intergenic
TTGATGGAGTTATCAATTCA+GGG 0.506860 1.2:+24701567 None:intergenic
TTTCTTCATTGGTCCCACTA+AGG 0.516843 1.2:-24699615 MS.gene026144:CDS
TGGTTATAAAATTGTATCAA+AGG 0.519033 1.2:+24701476 None:intergenic
GGTTAAGGGAAGGTCCTAGT+AGG 0.521835 1.2:-24701364 MS.gene026144:CDS
TGATAAAGGAGAGAAAGGTT+TGG 0.523809 1.2:-24699082 MS.gene026144:CDS
TATTGTTGCACTTGGTGAGT+TGG 0.527334 1.2:+24699569 None:intergenic
AACTCAAGTCATGGAGAGGT+TGG 0.531928 1.2:-24699040 MS.gene026144:CDS
AACCTCTCCATGACTTGAGT+TGG 0.536373 1.2:+24699042 None:intergenic
TCAACCATAAGTTTCCTACT+AGG 0.537063 1.2:+24701350 None:intergenic
CATGTTTCCCTTAGACGACA+TGG 0.537703 1.2:-24699648 MS.gene026144:CDS
CGAATTGCAGCTACCTTAGT+GGG 0.542842 1.2:+24699602 None:intergenic
CCCACGAGATATCTCTCCTA+AGG 0.551843 1.2:+24699787 None:intergenic
GTGTCTGATGTTACACGTGC+CGG 0.552169 1.2:-24699213 MS.gene026144:CDS
ACCATGATCCAAATATGGCA+GGG 0.552731 1.2:-24701457 MS.gene026144:CDS
CTCTAGTATTCAAAGTGAAG+AGG 0.559433 1.2:-24701595 MS.gene026144:CDS
GTTTATGGAGGACCAGCTAA+AGG 0.560640 1.2:-24699156 MS.gene026144:CDS
CGGTGAACCATGTCGTCTAA+GGG 0.561272 1.2:+24699641 None:intergenic
CATACAATGAGTGATGCATC+AGG 0.566369 1.2:-24699830 MS.gene026144:CDS
AAATTCAATGCATACTCCAA+TGG 0.567686 1.2:+24699342 None:intergenic
TTTACTATCCATTTGCAGGT+AGG 0.573968 1.2:-24701385 MS.gene026144:CDS
GCCGATTTCATGGTTATCAA+GGG 0.574726 1.2:-24699270 MS.gene026144:CDS
TGCCAACTCAAGTCATGGAG+AGG 0.581695 1.2:-24699044 MS.gene026144:CDS
CAGATTTACTATACGTGCAT+CGG 0.586133 1.2:+24699451 None:intergenic
AATTGGAGTATTGTTGCACT+TGG 0.588655 1.2:+24699561 None:intergenic
GAGTAAACCTTCAATCTCAC+CGG 0.590238 1.2:-24699762 MS.gene026144:CDS
AACATTAACCAGCCTACTAA+GGG 0.592477 1.2:-24698988 MS.gene026144:CDS
GCCTTAGGAGAGATATCTCG+TGG 0.593459 1.2:-24699788 MS.gene026144:CDS
GGACCAGCTAAAGGAGGGGA+TGG 0.594000 1.2:-24699147 MS.gene026144:CDS
CATGTCGTCTAAGGGAAACA+TGG 0.598974 1.2:+24699649 None:intergenic
ATGGAGGACCAGCTAAAGGA+GGG 0.603039 1.2:-24699152 MS.gene026144:CDS
TATACCTGGATAAGTAGCAA+TGG 0.605830 1.2:+24701185 None:intergenic
ATATGAAAGACAGCTTGACC+AGG 0.606019 1.2:+24699117 None:intergenic
AGATAAAACCACCACACTTG+AGG 0.612567 1.2:+24699870 None:intergenic
TAAAGAAGTTCATCCAAGCA+AGG 0.615141 1.2:+24701242 None:intergenic
TCATCCAAGCAAGGAAATGG+AGG 0.615425 1.2:+24701251 None:intergenic
ATGACCAAGATTCGTTACGT+TGG 0.616650 1.2:-24701505 MS.gene026144:CDS
CAGGTAACACGCCTCAAGTG+TGG 0.617684 1.2:-24699881 MS.gene026144:CDS
ATGGTTGATTGTAATGGTGA+AGG 0.618125 1.2:-24701335 MS.gene026144:CDS
TATGGAGGACCAGCTAAAGG+AGG 0.622508 1.2:-24699153 MS.gene026144:CDS
GTTGCACTTGGTGAGTTGGA+AGG 0.622784 1.2:+24699573 None:intergenic
GAACCAGAACCCGACAACAA+AGG 0.627957 1.2:-24699674 MS.gene026144:CDS
AACCTTCAATCTCACCGGTG+TGG 0.645966 1.2:-24699757 MS.gene026144:CDS
CAAAGAGTATATGCATTCAG+TGG 0.647914 1.2:-24699292 MS.gene026144:CDS
TTTGCAGGTAGGTTAAGGGA+AGG 0.651163 1.2:-24701374 MS.gene026144:CDS
CCTACTAAGGGTGATCACAA+TGG 0.657807 1.2:-24698976 MS.gene026144:CDS
TTAATCACATCTGAAGAACC+AGG 0.665982 1.2:+24701212 None:intergenic
AATCCAGCAGTAGTTAAAGG+AGG 0.671945 1.2:+24699243 None:intergenic
TGGGACCAATGAAGAAAGTG+CGG 0.687559 1.2:+24699621 None:intergenic
AGAAGCTCTCTTCTCCACAC+CGG 0.690241 1.2:+24699743 None:intergenic
GTAACACGCCTCAAGTGTGG+TGG 0.692143 1.2:-24699878 MS.gene026144:CDS
TGGAGGACCAGCTAAAGGAG+GGG 0.700713 1.2:-24699151 MS.gene026144:CDS
CCTTAGGAGAGATATCTCGT+GGG 0.713373 1.2:-24699787 MS.gene026144:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTAAAATTTTGTG+GGG - chr1.2:24700106-24700125 MS.gene026144:intron 10.0%
!!! AAATATTTTTTTATAAGGAT+TGG - chr1.2:24699792-24699811 MS.gene026144:CDS 10.0%
!!! AAATTTTTTATTAATTGAAG+TGG - chr1.2:24700482-24700501 MS.gene026144:intron 10.0%
!!! TTTAAACCTTATTTTTAATA+TGG + chr1.2:24699642-24699661 None:intergenic 10.0%
!! AAAATGATTCAAATATATTG+TGG - chr1.2:24700163-24700182 MS.gene026144:intron 15.0%
!! ATAAAAAGTAGTATATTTGT+AGG - chr1.2:24699885-24699904 MS.gene026144:intron 15.0%
!!! AAGTTGAAGTAATATTTTAT+AGG - chr1.2:24700194-24700213 MS.gene026144:intron 15.0%
!!! AGTTGAAGTAATATTTTATA+GGG - chr1.2:24700195-24700214 MS.gene026144:intron 15.0%
!!! ATATTTGAATCATTTTTTGA+AGG + chr1.2:24700159-24700178 None:intergenic 15.0%
!!! CAATTTATTTTATCTTTTCA+AGG - chr1.2:24699829-24699848 MS.gene026144:CDS 15.0%
!!! TTCAATTCATTTTAACTTTT+AGG + chr1.2:24699928-24699947 None:intergenic 15.0%
!! AAAACTTATTGAAAATCCAT+TGG - chr1.2:24701201-24701220 MS.gene026144:CDS 20.0%
!! CATGTTAAAAAATTGTTACA+AGG + chr1.2:24700385-24700404 None:intergenic 20.0%
!! GATATTACTCTTAAACAATT+TGG - chr1.2:24699272-24699291 MS.gene026144:CDS 20.0%
!! TAGAAAGTTCAAATCTTTAT+GGG + chr1.2:24700533-24700552 None:intergenic 20.0%
!! TATAACAAATGTAACATCAA+AGG + chr1.2:24700556-24700575 None:intergenic 20.0%
!! TGGTTATAAAATTGTATCAA+AGG + chr1.2:24699086-24699105 None:intergenic 20.0%
!! TTTAGGCCATATTAAAAATA+AGG - chr1.2:24699633-24699652 MS.gene026144:CDS 20.0%
!!! ATTATTTTAACAAGTGATGA+TGG + chr1.2:24700093-24700112 None:intergenic 20.0%
!!! ATTCCTTTTACAAATGATAA+AGG - chr1.2:24701463-24701482 MS.gene026144:CDS 20.0%
! AAAATCAACATCTCTAAATC+CGG + chr1.2:24701368-24701387 None:intergenic 25.0%
! AAACTTATGGTTGATTGTAA+TGG - chr1.2:24699218-24699237 MS.gene026144:CDS 25.0%
! AATCATAAAAGGTTTAAGCT+CGG + chr1.2:24700067-24700086 None:intergenic 25.0%
! AGAAAGTTCAAATCTTTATG+GGG + chr1.2:24700532-24700551 None:intergenic 25.0%
! ATGATTCAAATATATTGTGG+TGG - chr1.2:24700166-24700185 MS.gene026144:intron 25.0%
! GTAGAAAGTTCAAATCTTTA+TGG + chr1.2:24700534-24700553 None:intergenic 25.0%
! GTCCTTTCAATACTTATTTA+AGG + chr1.2:24699729-24699748 None:intergenic 25.0%
! TATGCAGTGAATTAAACTTA+AGG + chr1.2:24700599-24700618 None:intergenic 25.0%
! TCTCCTTTATCATTTGTAAA+AGG + chr1.2:24701469-24701488 None:intergenic 25.0%
! TGATTCAAATATATTGTGGT+GGG - chr1.2:24700167-24700186 MS.gene026144:intron 25.0%
! TTATAACCATGATCCAAATA+TGG - chr1.2:24699097-24699116 MS.gene026144:CDS 25.0%
!! AAAATGAATTGAAGCCTTAT+CGG - chr1.2:24699935-24699954 MS.gene026144:intron 25.0%
!! AAAGTTTTCCGATAATATGT+AGG + chr1.2:24699416-24699435 None:intergenic 25.0%
!! AAGTTTTCCGATAATATGTA+GGG + chr1.2:24699415-24699434 None:intergenic 25.0%
!! ACATTTTTATTGAGATCACA+CGG - chr1.2:24699485-24699504 MS.gene026144:CDS 25.0%
!!! ATCAAGAGTATTTAAAAGCT+TGG + chr1.2:24699968-24699987 None:intergenic 25.0%
!!! GATGATGGATTAATCATAAA+AGG + chr1.2:24700078-24700097 None:intergenic 25.0%
!!! GTCCTTAAATAAGTATTGAA+AGG - chr1.2:24699724-24699743 MS.gene026144:CDS 25.0%
!!! TTATTTTAAAATTGGGCAAG+TGG - chr1.2:24700459-24700478 MS.gene026144:intron 25.0%
AAATTCAATGCATACTCCAA+TGG + chr1.2:24701220-24701239 None:intergenic 30.0%
ACAAATGATAAAGGAGAGAA+AGG - chr1.2:24701472-24701491 MS.gene026144:CDS 30.0%
ATCTTTATGGGGGTTAAAAA+TGG + chr1.2:24700521-24700540 None:intergenic 30.0%
CACTTTGAATACTAGAGATT+TGG + chr1.2:24698961-24698980 None:intergenic 30.0%
GAAAAGATCAATTGTATACC+TGG + chr1.2:24699391-24699410 None:intergenic 30.0%
GAAAGTTCAAATCTTTATGG+GGG + chr1.2:24700531-24700550 None:intergenic 30.0%
TATAATCCAGCAGTAGTTAA+AGG + chr1.2:24701322-24701341 None:intergenic 30.0%
TGCAGCAATGATTTCAAAAT+TGG + chr1.2:24701018-24701037 None:intergenic 30.0%
TTGATGGAGTTATCAATTCA+GGG + chr1.2:24698995-24699014 None:intergenic 30.0%
TTTGATGGAGTTATCAATTC+AGG + chr1.2:24698996-24699015 None:intergenic 30.0%
TTTGTCAGAATCTAGTTGTA+AGG + chr1.2:24701066-24701085 None:intergenic 30.0%
! AAATCATTGCTGCATACTTT+TGG - chr1.2:24701024-24701043 MS.gene026144:intron 30.0%
! CTTTTCACCCTACATATTAT+CGG - chr1.2:24699405-24699424 MS.gene026144:intron 30.0%
! CTTTTCGAATAACATCAACA+GGG + chr1.2:24699133-24699152 None:intergenic 30.0%
!! AAGAGTATTTAAAAGCTTGG+TGG + chr1.2:24699965-24699984 None:intergenic 30.0%
!!! GGATTTAGAGATGTTGATTT+TGG - chr1.2:24701367-24701386 MS.gene026144:CDS 30.0%
!!! TAGAGATGTTGATTTTGGTT+GGG - chr1.2:24701372-24701391 MS.gene026144:CDS 30.0%
!!! TTAGAGATGTTGATTTTGGT+TGG - chr1.2:24701371-24701390 MS.gene026144:CDS 30.0%
AACATTAACCAGCCTACTAA+GGG - chr1.2:24701571-24701590 MS.gene026144:CDS 35.0%
ACGGGTGTGTATAAGTATAA+GGG + chr1.2:24700269-24700288 None:intergenic 35.0%
AGTAACCATTTGGAAGTTGT+TGG + chr1.2:24701138-24701157 None:intergenic 35.0%
ATGGTTGATTGTAATGGTGA+AGG - chr1.2:24699224-24699243 MS.gene026144:CDS 35.0%
CAACTTCCAAATGGTTACTA+TGG - chr1.2:24701139-24701158 MS.gene026144:intron 35.0%
CAGATTTACTATACGTGCAT+CGG + chr1.2:24701111-24701130 None:intergenic 35.0%
CGATCACAATCAAACACTTA+CGG + chr1.2:24700288-24700307 None:intergenic 35.0%
CTCTAGTATTCAAAGTGAAG+AGG - chr1.2:24698964-24698983 MS.gene026144:CDS 35.0%
CTTCAATTCTTGATTGCCTT+AGG - chr1.2:24700756-24700775 MS.gene026144:intron 35.0%
GAACTTCTTTATGATGTTCC+TGG - chr1.2:24699329-24699348 MS.gene026144:CDS 35.0%
GATCACAATCAAACACTTAC+GGG + chr1.2:24700287-24700306 None:intergenic 35.0%
GGAAAACTTTGCATTATAGC+CGG - chr1.2:24699426-24699445 MS.gene026144:intron 35.0%
TAAAGAAGTTCATCCAAGCA+AGG + chr1.2:24699320-24699339 None:intergenic 35.0%
TACGGGTGTGTATAAGTATA+AGG + chr1.2:24700270-24700289 None:intergenic 35.0%
TATACCTGGATAAGTAGCAA+TGG + chr1.2:24699377-24699396 None:intergenic 35.0%
TCAACCATAAGTTTCCTACT+AGG + chr1.2:24699212-24699231 None:intergenic 35.0%
TGATAAAGGAGAGAAAGGTT+TGG - chr1.2:24701477-24701496 MS.gene026144:CDS 35.0%
TTAATCACATCTGAAGAACC+AGG + chr1.2:24699350-24699369 None:intergenic 35.0%
TTTACTATCCATTTGCAGGT+AGG - chr1.2:24699174-24699193 MS.gene026144:CDS 35.0%
! AAACATGGTTCCTTTGTTGT+CGG + chr1.2:24700898-24700917 None:intergenic 35.0%
! ACCCTTGATAACCATGAAAT+CGG + chr1.2:24701293-24701312 None:intergenic 35.0%
! CAAAGAGTATATGCATTCAG+TGG - chr1.2:24701267-24701286 MS.gene026144:CDS 35.0%
! GAAAGCGTAAAAATCATGAC+CGG + chr1.2:24699448-24699467 None:intergenic 35.0%
! GCTTTTCGAATAACATCAAC+AGG + chr1.2:24699134-24699153 None:intergenic 35.0%
! GTGTTTTACTATCCATTTGC+AGG - chr1.2:24699170-24699189 MS.gene026144:CDS 35.0%
!! AATTGGAGTATTGTTGCACT+TGG + chr1.2:24701001-24701020 None:intergenic 35.0%
!! ACTTGAGTTGGCAAAAAAAC+TGG + chr1.2:24701508-24701527 None:intergenic 35.0%
!!! AGAGATGTTGATTTTGGTTG+GGG - chr1.2:24701373-24701392 MS.gene026144:CDS 35.0%
!!! AGTTTTGTTTCATGAGGTGT+GGG + chr1.2:24699020-24699039 None:intergenic 35.0%
!!! CGATGAGTACATGTTACTTT+TGG + chr1.2:24700858-24700877 None:intergenic 35.0%
!!! GAGAGTAGTTTTGTTTCATG+AGG + chr1.2:24699026-24699045 None:intergenic 35.0%
!!! TAGTTTTGTTTCATGAGGTG+TGG + chr1.2:24699021-24699040 None:intergenic 35.0%
!!! TGAGTACATGTTACTTTTGG+TGG + chr1.2:24700855-24700874 None:intergenic 35.0%
!!! TTTTTTGCCAACTCAAGTCA+TGG - chr1.2:24701510-24701529 MS.gene026144:CDS 35.0%
AACCATGATCCAAATATGGC+AGG - chr1.2:24699101-24699120 MS.gene026144:CDS 40.0%
AATCCAGCAGTAGTTAAAGG+AGG + chr1.2:24701319-24701338 None:intergenic 40.0%
ACCATGATCCAAATATGGCA+GGG - chr1.2:24699102-24699121 MS.gene026144:CDS 40.0%
AGATAAAACCACCACACTTG+AGG + chr1.2:24700692-24700711 None:intergenic 40.0%
AGGTCCTAGTAGGAAACTTA+TGG - chr1.2:24699205-24699224 MS.gene026144:CDS 40.0%
AGTTCATCCAAGCAAGGAAA+TGG + chr1.2:24699314-24699333 None:intergenic 40.0%
ATATGAAAGACAGCTTGACC+AGG + chr1.2:24701445-24701464 None:intergenic 40.0%
ATCCATTTGCAGGTAGGTTA+AGG - chr1.2:24699180-24699199 MS.gene026144:CDS 40.0%
ATGACCAAGATTCGTTACGT+TGG - chr1.2:24699054-24699073 MS.gene026144:CDS 40.0%
CAACATTAACCAGCCTACTA+AGG - chr1.2:24701570-24701589 MS.gene026144:CDS 40.0%
CACAAACACATCGAAACGAA+AGG + chr1.2:24699557-24699576 None:intergenic 40.0%
CATACAATGAGTGATGCATC+AGG - chr1.2:24700729-24700748 MS.gene026144:intron 40.0%
CTGATGCATCACTCATTGTA+TGG + chr1.2:24700731-24700750 None:intergenic 40.0%
CTTGAATTGTAACTACGTGC+AGG - chr1.2:24700659-24700678 MS.gene026144:intron 40.0%
GAGTAAACCTTCAATCTCAC+CGG - chr1.2:24700797-24700816 MS.gene026144:intron 40.0%
GCGTTACCATAGTAACCATT+TGG + chr1.2:24701148-24701167 None:intergenic 40.0%
GTTGACCAACAACTTCCAAA+TGG - chr1.2:24701130-24701149 MS.gene026144:intron 40.0%
TCCATTTGCAGGTAGGTTAA+GGG - chr1.2:24699181-24699200 MS.gene026144:CDS 40.0%
TTTCTTCATTGGTCCCACTA+AGG - chr1.2:24700944-24700963 MS.gene026144:intron 40.0%
! AACATGGTTCCTTTGTTGTC+GGG + chr1.2:24700897-24700916 None:intergenic 40.0%
! GCCGATTTCATGGTTATCAA+GGG - chr1.2:24701289-24701308 MS.gene026144:CDS 40.0%
! TATTGTTGCACTTGGTGAGT+TGG + chr1.2:24700993-24701012 None:intergenic 40.0%
!!! GAGATGTTGATTTTGGTTGG+GGG - chr1.2:24701374-24701393 MS.gene026144:CDS 40.0%
AACCTCTCCATGACTTGAGT+TGG + chr1.2:24701520-24701539 None:intergenic 45.0%
AACTCAAGTCATGGAGAGGT+TGG - chr1.2:24701519-24701538 MS.gene026144:CDS 45.0%
CATGTCGTCTAAGGGAAACA+TGG + chr1.2:24700913-24700932 None:intergenic 45.0%
CATGTTTCCCTTAGACGACA+TGG - chr1.2:24700911-24700930 MS.gene026144:intron 45.0%
CCATTGTGATCACCCTTAGT+AGG + chr1.2:24701586-24701605 None:intergenic 45.0%
CCTACTAAGGGTGATCACAA+TGG - chr1.2:24701583-24701602 MS.gene026144:CDS 45.0%
CCTTAGGAGAGATATCTCGT+GGG - chr1.2:24700772-24700791 MS.gene026144:intron 45.0%
CGAATTGCAGCTACCTTAGT+GGG + chr1.2:24700960-24700979 None:intergenic 45.0%
CTTGCCAACGTAACGAATCT+TGG + chr1.2:24699061-24699080 None:intergenic 45.0%
GTTCACCGCACTTTCTTCAT+TGG - chr1.2:24700933-24700952 MS.gene026144:intron 45.0%
GTTTATGGAGGACCAGCTAA+AGG - chr1.2:24701403-24701422 MS.gene026144:CDS 45.0%
TACGCTTTCACGTTGTCTCT+AGG - chr1.2:24699459-24699478 MS.gene026144:intron 45.0%
TCATCCAAGCAAGGAAATGG+AGG + chr1.2:24699311-24699330 None:intergenic 45.0%
TCATCCTCCATTTCCTTGCT+TGG - chr1.2:24699304-24699323 MS.gene026144:CDS 45.0%
TCCCTGCCATATTTGGATCA+TGG + chr1.2:24699106-24699125 None:intergenic 45.0%
TCCCTTAACCTACCTGCAAA+TGG + chr1.2:24699185-24699204 None:intergenic 45.0%
TGGGACCAATGAAGAAAGTG+CGG + chr1.2:24700941-24700960 None:intergenic 45.0%
TTTGCAGGTAGGTTAAGGGA+AGG - chr1.2:24699185-24699204 MS.gene026144:CDS 45.0%
! AGCTTGGTGGTATTCCGATA+AGG + chr1.2:24699952-24699971 None:intergenic 45.0%
! CACTCCATTGCTACTTATCC+AGG - chr1.2:24699370-24699389 MS.gene026144:intron 45.0%
! GGAGAGGTTGGTTAAAGAGT+TGG - chr1.2:24701531-24701550 MS.gene026144:CDS 45.0%
! GGCCGATTTCATGGTTATCA+AGG - chr1.2:24701288-24701307 MS.gene026144:CDS 45.0%
!! TCATGAGGTGTGGGTTTTGA+TGG + chr1.2:24699011-24699030 None:intergenic 45.0%
!!! AAAATAATTTAAAATTTTGT+GGG - chr1.2:24700105-24700124 MS.gene026144:intron 5.0%
!!! ACATAAAATATTTTTTTATA+AGG - chr1.2:24699787-24699806 MS.gene026144:CDS 5.0%
!!! ACTAAAATTTTAAATTTTAT+AGG - chr1.2:24700225-24700244 MS.gene026144:intron 5.0%
!!! ATTTTATATCATTTAATTTT+AGG - chr1.2:24699616-24699635 MS.gene026144:CDS 5.0%
!!! CAAATTTTTATTTTAAAATT+GGG - chr1.2:24700452-24700471 MS.gene026144:intron 5.0%
!!! TAAAATAATTTAAAATTTTG+TGG - chr1.2:24700104-24700123 MS.gene026144:intron 5.0%
!!! TATAAAAAAATATTTTATGT+TGG + chr1.2:24699787-24699806 None:intergenic 5.0%
!!! TCAAATTTTTATTTTAAAAT+TGG - chr1.2:24700451-24700470 MS.gene026144:intron 5.0%
!!! TTTTAAAATAAAAATTTGAT+AGG + chr1.2:24700450-24700469 None:intergenic 5.0%
AACCTTCAATCTCACCGGTG+TGG - chr1.2:24700802-24700821 MS.gene026144:intron 50.0%
AGAAGCTCTCTTCTCCACAC+CGG + chr1.2:24700819-24700838 None:intergenic 50.0%
ATGGAGGACCAGCTAAAGGA+GGG - chr1.2:24701407-24701426 MS.gene026144:CDS 50.0%
CCCACGAGATATCTCTCCTA+AGG + chr1.2:24700775-24700794 None:intergenic 50.0%
CGCTTTAGCATTTCCCGTTG+CGG - chr1.2:24701165-24701184 MS.gene026144:intron 50.0%
CGGTGAACCATGTCGTCTAA+GGG + chr1.2:24700921-24700940 None:intergenic 50.0%
CGTCCTCCTTTAACTACTGC+TGG - chr1.2:24701313-24701332 MS.gene026144:CDS 50.0%
GAACCAGAACCCGACAACAA+AGG - chr1.2:24700885-24700904 MS.gene026144:intron 50.0%
GCCTTAGGAGAGATATCTCG+TGG - chr1.2:24700771-24700790 MS.gene026144:intron 50.0%
GCGAATTGCAGCTACCTTAG+TGG + chr1.2:24700961-24700980 None:intergenic 50.0%
GGGTCTTTCCCTGCCATATT+TGG + chr1.2:24699113-24699132 None:intergenic 50.0%
GGTTAAGGGAAGGTCCTAGT+AGG - chr1.2:24699195-24699214 MS.gene026144:CDS 50.0%
GGTTGGGGGAAAGCTGTTTA+TGG - chr1.2:24701388-24701407 MS.gene026144:CDS 50.0%
GTGTCTGATGTTACACGTGC+CGG - chr1.2:24701346-24701365 MS.gene026144:CDS 50.0%
TATGGAGGACCAGCTAAAGG+AGG - chr1.2:24701406-24701425 MS.gene026144:CDS 50.0%
TCCTGCAGTTGTAACCGCAA+CGG + chr1.2:24701182-24701201 None:intergenic 50.0%
TGCCAACTCAAGTCATGGAG+AGG - chr1.2:24701515-24701534 MS.gene026144:CDS 50.0%
TGGGGGAAAGCTGTTTATGG+AGG - chr1.2:24701391-24701410 MS.gene026144:CDS 50.0%
TGTGATCACCCTTAGTAGGC+TGG + chr1.2:24701582-24701601 None:intergenic 50.0%
! GCATTCAGTGGCCGATTTCA+TGG - chr1.2:24701279-24701298 MS.gene026144:CDS 50.0%
! GTTCCTTTGTTGTCGGGTTC+TGG + chr1.2:24700891-24700910 None:intergenic 50.0%
! GTTGCACTTGGTGAGTTGGA+AGG + chr1.2:24700989-24701008 None:intergenic 50.0%
CAGGTAACACGCCTCAAGTG+TGG - chr1.2:24700678-24700697 MS.gene026144:intron 55.0%
CCTGCAGTTGTAACCGCAAC+GGG + chr1.2:24701181-24701200 None:intergenic 55.0%
CTCCACACCGGTGAGATTGA+AGG + chr1.2:24700807-24700826 None:intergenic 55.0%
GCGGTGAACCATGTCGTCTA+AGG + chr1.2:24700922-24700941 None:intergenic 55.0%
GTAACACGCCTCAAGTGTGG+TGG - chr1.2:24700681-24700700 MS.gene026144:intron 55.0%
TGGAGGACCAGCTAAAGGAG+GGG - chr1.2:24701408-24701427 MS.gene026144:CDS 55.0%
CCCGTTGCGGTTACAACTGC+AGG - chr1.2:24701178-24701197 MS.gene026144:intron 60.0%
GCACCATCCCCTCCTTTAGC+TGG + chr1.2:24701418-24701437 None:intergenic 60.0%
GGACCAGCTAAAGGAGGGGA+TGG - chr1.2:24701412-24701431 MS.gene026144:CDS 60.0%
GGAGGGGATGGTGCTCTTCC+TGG - chr1.2:24701424-24701443 MS.gene026144:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 24698954 24701627 24698954 ID=MS.gene026144
chr1.2 mRNA 24698954 24701627 24698954 ID=MS.gene026144.t1;Parent=MS.gene026144
chr1.2 exon 24701190 24701627 24701190 ID=MS.gene026144.t1.exon1;Parent=MS.gene026144.t1
chr1.2 CDS 24701190 24701627 24701190 ID=cds.MS.gene026144.t1;Parent=MS.gene026144.t1
chr1.2 exon 24699465 24699900 24699465 ID=MS.gene026144.t1.exon2;Parent=MS.gene026144.t1
chr1.2 CDS 24699465 24699900 24699465 ID=cds.MS.gene026144.t1;Parent=MS.gene026144.t1
chr1.2 exon 24698954 24699381 24698954 ID=MS.gene026144.t1.exon3;Parent=MS.gene026144.t1
chr1.2 CDS 24698954 24699381 24698954 ID=cds.MS.gene026144.t1;Parent=MS.gene026144.t1
Gene Sequence

>MS.gene026144

ATGGCCAAATCTCTAGTATTCAAAGTGAAGAGGCGTGACCCTGAATTGATAACTCCATCAAAACCCACACCTCATGAAACAAAACTACTCTCAGACATTGATGACCAAGATTCGTTACGTTGGCAAGTTCCTTTGATACAATTTTATAACCATGATCCAAATATGGCAGGGAAAGACCCTGTTGATGTTATTCGAAAAGCATTAGCAAAAACACTTGTGTTTTACTATCCATTTGCAGGTAGGTTAAGGGAAGGTCCTAGTAGGAAACTTATGGTTGATTGTAATGGTGAAGGTGTTTTGTTCATTGAAGCTGATGCTGATATTACTCTTAAACAATTTGGTGATGCTCTTCATCCTCCATTTCCTTGCTTGGATGAACTTCTTTATGATGTTCCTGGTTCTTCAGATGTGATTAACACTCCATTGCTACTTATCCAGGTATACAATTGATCTTTTCACCCTACATATTATCGGAAAACTTTGCATTATAGCCGGTCATGATTTTTACGCTTTCACGTTGTCTCTAGGTGAACATTTTTATTGAGATCACACGGTTCATATTTTAAAATTTTTCTTCAAAATCTGCTTTGAATTTTGACTCCTTTCGTTTCGATGTGTTTGTGTGATTCTAATTTTTTTATTTGATTTTAAGTCTCTAAAATATTTTATATCATTTAATTTTAGGCCATATTAAAAATAAGGTTTAAATATAGTTTTGTTACTTGCAAATATACAATTTTTTTTTGTTTTAATCTTTACAAAATTTGTTAGTCCTTAAATAAGTATTGAAAGGACGAAAACTAAGTATTCTACATATTACAAGATCAATTCCAACATAAAATATTTTTTTATAAGGATTGGATTATGATGAAAAGCAATTTATTTTATCTTTTCAAGGTCTAAATAAAAGATATTTTCACAAGTATGAAAAATAAAAAGTAGTATATTTGTAGGACTAAAAGATATTTAAACCTAAAAGTTAAAATGAATTGAAGCCTTATCGGAATACCACCAAGCTTTTAAATACTCTTGATGTGTTATCATAGAAGTCATTGTCAAAACGATCTCAAAATTTGATTTTTAGATCTATATTTTAGTTTCTTGAGTTTTCCGAGCTTAAACCTTTTATGATTAATCCATCATCACTTGTTAAAATAATTTAAAATTTTGTGGGGAGTGTTGTTTATAATGTTCTAAACATGCCTTCAAAAAATGATTCAAATATATTGTGGTGGGCAAAAAGTTGAAGTAATATTTTATAGGGACTAAAGACTAAAATTTTAAATTTTATAGGCACTAAAAATATATTTAACCCTTATACTTATACACACCCGTAAGTGTTTGATTGTGATCGAATTTTTATTATTTTGTAATTTATAACAATTTTTATTTTTTCACTATTTTTATGCATTTATCAAATCATATTCACCTTGTAACAATTTTTTAACATGTCTTAGAAATTAACATTATGTGTATAAATAAATTATGCACTGCCTATCAAATTTTTATTTTAAAATTGGGCAAGTGGAAATTTTTTATTAATTGAAGTGGTACTATTGAATGACCATTTTTAACCCCCATAAAGATTTGAACTTTCTACCTTTGATGTTACATTTGTTATATTTTTTCAACTAAGCTAGAACCTTAAGTTTAATTCACTGCATATCAATTGATCTTAATAAATCTTTAATCATTCATTAACATACTTGAATTGTAACTACGTGCAGGTAACACGCCTCAAGTGTGGTGGTTTTATCTTTGCTCTTCGTATGAACCATACAATGAGTGATGCATCAGGTATGCTTCAATTCTTGATTGCCTTAGGAGAGATATCTCGTGGGATGAGTAAACCTTCAATCTCACCGGTGTGGAGAAGAGAGCTTCTTAACGCAAGAGATCCACCAAAAGTAACATGTACTCATCGTGAATATGAACCAGAACCCGACAACAAAGGAACCATGTTTCCCTTAGACGACATGGTTCACCGCACTTTCTTCATTGGTCCCACTAAGGTAGCTGCAATTCGCTCTCTCCTTCCAACTCACCAAGTGCAACAATACTCCAATTTTGAAATCATTGCTGCATACTTTTGGCGTTGTCGTACAAAAGCCTTACAACTAGATTCTGACAAAGAAGTTCGCATAATCTGCATAGCCGATGCACGTATAGTAAATCTGTTGACCAACAACTTCCAAATGGTTACTATGGTAACGCTTTAGCATTTCCCGTTGCGGTTACAACTGCAGGAAAACTTATTGAAAATCCATTGGAGTATGCATTGAATTTAGTTAAGAAAGCAAAAACTAATGTCAACAAAGAGTATATGCATTCAGTGGCCGATTTCATGGTTATCAAGGGTCGTCCTCCTTTAACTACTGCTGGATTATATTTAGTGTCTGATGTTACACGTGCCGGATTTAGAGATGTTGATTTTGGTTGGGGGAAAGCTGTTTATGGAGGACCAGCTAAAGGAGGGGATGGTGCTCTTCCTGGTCAAGCTGTCTTTCATATTCCTTTTACAAATGATAAAGGAGAGAAAGGTTTGGTTATACCAGTTTTTTTGCCAACTCAAGTCATGGAGAGGTTGGTTAAAGAGTTGGATAGTGTTCTTAAGAACAACATTAACCAGCCTACTAAGGGTGATCACAATGGCATCATTAAATCTTCGTTGTAG
Protein sequence

>MS.gene026144.t1

MAKSLVFKVKRRDPELITPSKPTPHETKLLSDIDDQDSLRWQVPLIQFYNHDPNMAGKDPVDVIRKALAKTLVFYYPFAGRLREGPSRKLMVDCNGEGVLFIEADADITLKQFGDALHPPFPCLDELLYDVPGSSDVINTPLLLIQVTRLKCGGFIFALRMNHTMSDASGMLQFLIALGEISRGMSKPSISPVWRRELLNARDPPKVTCTHREYEPEPDNKGTMFPLDDMVHRTFFIGPTKVAAIRSLLPTHQVQQYSNFEIIAAYFWRCRTKALQLDSDKEVRIICIADARKLIENPLEYALNLVKKAKTNVNKEYMHSVADFMVIKGRPPLTTAGLYLVSDVTRAGFRDVDFGWGKAVYGGPAKGGDGALPGQAVFHIPFTNDKGEKGLVIPVFLPTQVMERLVKELDSVLKNNINQPTKGDHNGIIKSSL