AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38361

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38361.t1 MTR_3g101930 76.242 463 99 4 1 461 1 454 0.0 741
MS.gene38361.t1 MTR_3g101900 74.730 463 103 4 1 463 1 449 0.0 715
MS.gene38361.t1 MTR_3g102020 67.763 456 137 4 10 465 9 454 0.0 642
MS.gene38361.t1 MTR_3g101960 68.527 448 130 5 10 457 9 445 0.0 636
MS.gene38361.t1 MTR_3g102010 66.026 468 142 6 1 465 1 454 0.0 628
MS.gene38361.t1 MTR_3g101970 64.978 454 135 4 10 463 9 438 0.0 619
MS.gene38361.t1 MTR_3g102030 57.317 328 104 6 88 415 29 320 4.68e-131 382
MS.gene38361.t1 MTR_4g015180 37.282 515 245 10 2 464 1 489 2.05e-94 294
MS.gene38361.t1 MTR_4g094842 39.956 453 240 14 17 459 15 445 1.46e-92 288
MS.gene38361.t1 MTR_3g070170 35.976 492 244 10 4 458 9 466 4.20e-90 282
MS.gene38361.t1 MTR_5g081960 35.616 511 246 12 2 458 1 482 3.04e-89 281
MS.gene38361.t1 MTR_3g088420 38.202 445 240 9 24 462 18 433 2.68e-87 274
MS.gene38361.t1 MTR_4g022800 37.445 454 244 12 22 462 16 442 5.63e-87 273
MS.gene38361.t1 MTR_0190s0020 37.389 452 249 11 19 462 17 442 1.95e-85 270
MS.gene38361.t1 MTR_4g022670 37.748 453 243 12 22 462 16 441 6.04e-84 265
MS.gene38361.t1 MTR_8g041770 36.552 435 251 8 30 462 30 441 8.24e-84 265
MS.gene38361.t1 MTR_4g022720 38.479 447 242 10 22 462 16 435 9.59e-84 265
MS.gene38361.t1 MTR_4g094835 36.818 440 246 10 26 458 28 442 1.59e-81 259
MS.gene38361.t1 MTR_4g094838 35.240 437 239 10 30 458 28 428 2.46e-79 253
MS.gene38361.t1 MTR_4g022825 36.854 445 241 11 30 462 24 440 2.85e-78 251
MS.gene38361.t1 MTR_3g026310 36.748 449 244 11 30 462 24 448 3.14e-77 248
MS.gene38361.t1 MTR_7g026860 35.903 454 247 13 22 462 16 438 5.15e-77 248
MS.gene38361.t1 MTR_4g094828 35.532 470 262 13 10 463 7 451 6.21e-77 248
MS.gene38361.t1 MTR_4g022290 33.120 468 269 11 8 460 5 443 4.88e-71 232
MS.gene38361.t1 MTR_4g094832 35.784 408 232 10 63 458 37 426 2.03e-69 228
MS.gene38361.t1 MTR_8g079710 34.842 442 248 14 22 451 21 434 1.11e-66 221
MS.gene38361.t1 MTR_4g022760 37.057 367 195 9 22 376 16 358 1.24e-63 211
MS.gene38361.t1 MTR_6g045553 29.735 491 271 10 11 461 18 474 1.98e-56 194
MS.gene38361.t1 MTR_6g016815 30.664 437 206 14 30 462 38 381 4.66e-42 154
MS.gene38361.t1 MTR_8g040780 36.842 209 128 3 252 457 20 227 2.69e-37 137
MS.gene38361.t1 MTR_4g007540 28.571 413 232 16 56 443 44 418 7.79e-36 138
MS.gene38361.t1 MTR_8g075610 28.052 385 240 13 70 443 47 405 1.03e-35 137
MS.gene38361.t1 MTR_2g064495 28.369 423 237 15 56 452 44 426 1.30e-35 137
MS.gene38361.t1 MTR_2g105330 28.132 423 238 15 56 452 44 426 4.10e-35 135
MS.gene38361.t1 MTR_3g098980 31.818 308 181 11 70 369 66 352 3.90e-34 133
MS.gene38361.t1 MTR_8g096530 30.573 314 190 10 64 369 53 346 7.98e-33 129
MS.gene38361.t1 MTR_3g049150 26.420 405 250 11 57 437 45 425 2.56e-31 125
MS.gene38361.t1 MTR_7g026770 39.106 179 86 2 165 343 132 287 1.63e-29 117
MS.gene38361.t1 MTR_7g026850 41.250 160 70 5 44 200 21 159 9.27e-29 112
MS.gene38361.t1 MTR_3g026950 25.545 321 201 8 67 369 51 351 3.37e-27 114
MS.gene38361.t1 MTR_4g127650 25.474 475 285 19 10 452 12 449 1.66e-26 112
MS.gene38361.t1 MTR_3g087640 27.907 430 242 16 56 443 53 456 2.37e-23 102
MS.gene38361.t1 MTR_6g053810 27.796 313 197 10 70 373 71 363 4.63e-23 102
MS.gene38361.t1 MTR_7g114520 26.108 406 255 17 70 458 66 443 6.22e-23 101
MS.gene38361.t1 MTR_4g097090 24.236 458 286 14 14 443 16 440 6.96e-23 101
MS.gene38361.t1 MTR_4g094825 35.333 150 86 2 313 462 253 391 1.14e-22 100
MS.gene38361.t1 MTR_4g094825 34.682 173 99 4 30 202 92 250 1.49e-21 96.7
MS.gene38361.t1 MTR_7g027175 25.272 368 240 12 57 416 55 395 1.28e-22 100
MS.gene38361.t1 MTR_7g114550 25.581 430 279 16 44 458 37 440 2.00e-22 100
MS.gene38361.t1 MTR_8g040760 32.743 226 131 5 10 234 7 212 9.00e-22 94.0
MS.gene38361.t1 MTR_1g040195 25.946 370 237 13 68 430 37 376 4.64e-21 95.9
MS.gene38361.t1 MTR_5g076700 24.074 432 284 17 44 458 37 441 6.87e-21 95.5
MS.gene38361.t1 MTR_1g040175 26.346 353 220 15 70 411 64 387 1.16e-20 94.7
MS.gene38361.t1 MTR_7g086000 27.494 411 244 17 73 452 63 450 4.81e-20 92.8
MS.gene38361.t1 MTR_3g071320 27.119 354 218 10 69 407 59 387 5.29e-20 92.4
MS.gene38361.t1 MTR_1g053315 24.724 453 284 21 15 443 26 445 7.07e-20 92.4
MS.gene38361.t1 MTR_6g034495 40.625 128 64 5 81 205 45 163 7.61e-20 87.0
MS.gene38361.t1 MTR_7g114560 25.058 431 280 16 44 458 37 440 1.10e-19 91.7
MS.gene38361.t1 MTR_7g114960 25.339 442 290 14 30 458 30 444 1.92e-19 90.9
MS.gene38361.t1 MTR_1g081660 27.171 357 218 16 67 411 70 396 2.97e-19 90.5
MS.gene38361.t1 MTR_3g465980 26.518 313 189 12 48 346 49 334 4.37e-19 90.1
MS.gene38361.t1 MTR_3g465990 27.063 303 183 11 58 346 56 334 5.29e-19 89.7
MS.gene38361.t1 MTR_4g013050 25.939 293 167 8 68 357 51 296 7.87e-19 89.0
MS.gene38361.t1 MTR_7g085930 24.256 437 255 16 56 448 46 450 9.37e-19 89.0
MS.gene38361.t1 MTR_4g055170 26.736 288 180 9 70 346 69 336 1.40e-18 88.6
MS.gene38361.t1 MTR_1g040215 24.208 442 286 19 2 431 1 405 2.00e-18 87.8
MS.gene38361.t1 MTR_7g114470 23.636 440 277 18 44 458 38 443 2.32e-18 87.8
MS.gene38361.t1 MTR_7g084940 25.776 322 206 11 64 374 63 362 7.32e-18 86.3
MS.gene38361.t1 MTR_6g034485 34.677 124 81 0 216 339 2 125 2.10e-17 78.6
MS.gene38361.t1 MTR_3g112180 24.658 365 223 10 67 411 58 390 3.19e-17 84.3
MS.gene38361.t1 MTR_3g450710 23.238 383 229 14 64 411 59 411 4.38e-17 84.0
MS.gene38361.t1 MTR_3g028210 23.922 464 303 16 6 441 7 448 8.29e-16 80.1
MS.gene38361.t1 MTR_7g014330 25.791 411 254 16 30 413 26 412 2.03e-15 78.6
MS.gene38361.t1 MTR_3g049140 25.714 210 135 5 245 437 18 223 3.79e-15 75.5
MS.gene38361.t1 MTR_6g015815 23.819 487 304 19 1 453 1 454 4.16e-15 77.8
MS.gene38361.t1 MTR_1g040315 25.284 352 208 14 2 337 1 313 7.58e-15 76.6
MS.gene38361.t1 MTR_7g013850 23.261 460 279 18 31 441 30 464 1.10e-14 76.6
MS.gene38361.t1 MTR_5g090810 23.421 380 220 16 70 411 66 412 1.80e-14 75.9
MS.gene38361.t1 MTR_3g465930 24.912 285 190 6 70 346 69 337 1.95e-14 75.9
MS.gene38361.t1 MTR_6g015850 24.769 432 253 16 57 453 57 451 4.44e-14 74.7
MS.gene38361.t1 MTR_3g450690 22.222 378 234 15 65 411 67 415 8.87e-14 73.6
MS.gene38361.t1 MTR_2g015430 23.077 312 198 10 73 369 61 345 9.81e-14 73.6
MS.gene38361.t1 MTR_8g079970 25.234 428 259 22 56 463 46 432 1.18e-13 73.2
MS.gene38361.t1 MTR_3g465910 25.000 292 177 10 71 346 69 334 1.87e-13 72.8
MS.gene38361.t1 MTR_7g026830 55.556 54 24 0 136 189 44 97 1.88e-13 66.6
MS.gene38361.t1 MTR_6g015830 23.223 422 275 13 57 453 57 454 2.88e-13 72.0
MS.gene38361.t1 MTR_7g056087 23.145 337 220 13 56 374 45 360 3.09e-13 72.0
MS.gene38361.t1 MTR_7g084910 25.725 276 172 10 64 327 64 318 3.86e-13 71.6
MS.gene38361.t1 MTR_2g089650 22.897 428 250 19 30 413 29 420 4.81e-13 71.2
MS.gene38361.t1 MTR_3g465880 26.736 288 182 10 71 346 69 339 8.73e-13 70.5
MS.gene38361.t1 MTR_2g089670 23.177 384 242 15 56 409 68 428 1.23e-12 70.1
MS.gene38361.t1 MTR_2g045540 32.990 97 57 1 245 341 18 106 6.97e-12 62.8
MS.gene38361.t1 MTR_6g015315 24.641 418 243 17 62 443 55 436 2.18e-11 66.2
MS.gene38361.t1 MTR_7g014620 24.222 450 261 20 30 444 15 419 8.17e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38361.t1 AT5G42830 54.204 452 190 5 14 463 14 450 6.14e-172 491
MS.gene38361.t1 AT5G07850 52.495 461 207 7 3 462 4 453 2.04e-166 477
MS.gene38361.t1 AT5G07870 48.723 470 219 6 3 462 4 461 2.83e-146 426
MS.gene38361.t1 AT5G07860 49.448 453 213 6 10 461 11 448 1.93e-145 424
MS.gene38361.t1 AT2G39980 37.866 478 218 12 34 462 33 480 1.00e-94 295
MS.gene38361.t1 AT5G67150 36.304 460 269 9 10 464 8 448 3.47e-91 285
MS.gene38361.t1 AT5G01210 36.853 502 252 11 1 466 1 473 6.29e-89 280
MS.gene38361.t1 AT3G50270 32.976 467 274 12 10 463 9 449 2.99e-79 254
MS.gene38361.t1 AT3G50280 32.747 455 278 9 10 458 7 439 2.28e-77 248
MS.gene38361.t1 AT5G67160 34.004 447 242 14 30 464 29 434 3.37e-71 232
MS.gene38361.t1 AT3G50300 32.653 441 259 12 10 439 7 420 6.34e-71 232
MS.gene38361.t1 AT5G38130 33.560 441 261 14 10 443 27 442 1.19e-69 229
MS.gene38361.t1 AT3G50280 36.688 308 184 5 156 458 8 309 1.42e-60 201
MS.gene38361.t1 AT5G23940 31.087 460 289 9 10 458 6 448 1.00e-58 201
MS.gene38361.t1 AT3G50290 39.464 261 143 6 157 408 15 269 5.16e-53 179
MS.gene38361.t1 AT5G48930 28.750 400 244 13 68 452 54 427 5.69e-36 138
MS.gene38361.t1 AT3G48720 32.440 336 192 12 29 357 29 336 3.99e-35 136
MS.gene38361.t1 AT5G41040 28.609 381 223 17 64 428 63 410 6.00e-34 133
MS.gene38361.t1 AT5G41040 28.609 381 223 17 64 428 79 426 7.39e-34 133
MS.gene38361.t1 AT5G63560 28.536 403 234 16 22 412 22 382 2.19e-31 125
MS.gene38361.t1 AT2G19070 27.876 452 265 20 30 452 25 444 4.88e-31 125
MS.gene38361.t1 AT5G57840 27.171 449 256 20 29 449 23 428 1.63e-22 100
MS.gene38361.t1 AT2G40230 26.718 393 247 13 40 421 35 397 5.94e-22 98.6
MS.gene38361.t1 AT1G03940 25.287 435 258 23 57 453 53 458 1.36e-21 97.8
MS.gene38361.t1 AT1G03495 28.481 316 169 18 57 338 53 345 5.63e-20 91.3
MS.gene38361.t1 AT1G28680 24.595 309 202 12 73 374 65 349 5.97e-20 92.0
MS.gene38361.t1 AT1G28680 24.595 309 202 12 73 374 65 349 7.42e-20 92.4
MS.gene38361.t1 AT1G78990 25.474 369 213 13 68 411 65 396 7.53e-20 92.4
MS.gene38361.t1 AT1G31490 26.118 425 251 18 56 457 48 432 2.96e-18 87.4
MS.gene38361.t1 AT3G26040 24.155 414 258 13 67 463 56 430 4.26e-18 87.0
MS.gene38361.t1 AT3G29635 25.783 415 235 20 30 409 29 405 6.49e-17 83.2
MS.gene38361.t1 AT1G03390 24.939 413 263 15 55 448 64 448 6.63e-17 83.2
MS.gene38361.t1 AT1G27620 24.931 361 218 12 70 411 58 384 1.05e-16 82.8
MS.gene38361.t1 AT1G24420 26.452 465 289 21 10 463 8 430 1.55e-16 82.0
MS.gene38361.t1 AT1G24420 26.452 465 289 21 10 463 8 430 1.55e-16 82.0
MS.gene38361.t1 AT5G07080 24.172 302 189 11 55 341 55 331 3.06e-16 81.3
MS.gene38361.t1 AT5G17540 25.545 321 199 12 44 349 36 331 1.37e-15 79.3
MS.gene38361.t1 AT2G25150 26.032 315 198 11 70 371 67 359 4.21e-15 77.8
MS.gene38361.t1 AT1G65450 29.412 187 110 6 176 347 1 180 7.20e-14 72.4
MS.gene38361.t1 AT3G29590 24.679 389 247 15 77 443 68 432 5.49e-13 71.2
MS.gene38361.t1 AT5G39080 21.413 467 281 15 30 451 28 453 9.68e-13 70.5

Find 105 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTGATCTATTGTTTA+TGG 0.126098 3.2:+84843320 None:intergenic
AAATAATTTCTCTCTCAATT+TGG 0.143020 3.2:+84843503 None:intergenic
TTCCATTTCTATCCTCTTTC+CGG 0.165652 3.2:-84844011 MS.gene38361:CDS
ATTTGTATTGGATTCTTTAT+TGG 0.177909 3.2:+84843440 None:intergenic
ATGAAATCTTGTTGGTTATT+TGG 0.209059 3.2:+84844080 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.211414 3.2:-84843051 MS.gene38361:CDS
TTTGGAATTTCTTCAATATA+TGG 0.257098 3.2:-84843677 MS.gene38361:CDS
GAATTGCTTGAGAATGATTT+AGG 0.262759 3.2:-84843228 MS.gene38361:CDS
AAGCAAATTTGTTACTTAAC+AGG 0.274512 3.2:-84844173 MS.gene38361:CDS
ATCTTCCTTCCAATCTTTAT+CGG 0.278893 3.2:-84843412 MS.gene38361:CDS
ATTTGCTTTGAATCTTCAAT+AGG 0.290643 3.2:+84844188 None:intergenic
AATAATTTCTCTCTCAATTT+GGG 0.299647 3.2:+84843504 None:intergenic
TTAGTGGACCGTAACCTTCT+AGG 0.322324 3.2:+84843580 None:intergenic
ATCAACAAACATGGTATAAG+AGG 0.329053 3.2:+84843956 None:intergenic
AATCTTTATCGGCATTTGTT+TGG 0.337842 3.2:-84843401 MS.gene38361:CDS
AGATTTCATTCAGAATCTAT+TGG 0.344488 3.2:-84844066 MS.gene38361:CDS
CATAATCGGTGGTTCCTAGA+AGG 0.366417 3.2:-84843594 MS.gene38361:CDS
TCACCAAGGTTCAATATGTA+TGG 0.368741 3.2:-84843063 MS.gene38361:CDS
TTTGCTTTGAATCTTCAATA+GGG 0.371311 3.2:+84844189 None:intergenic
GATTCTGAATGAAATCTTGT+TGG 0.378418 3.2:+84844072 None:intergenic
AGGAGGAGGAAGCATGGATT+TGG 0.380210 3.2:-84842950 MS.gene38361:CDS
CATGCCGTTGCTATCCATTC+AGG 0.380410 3.2:-84843769 MS.gene38361:CDS
AATGAGAACATCATCGCCTT+TGG 0.401999 3.2:+84843632 None:intergenic
AAACAAATGCCGATAAAGAT+TGG 0.404666 3.2:+84843403 None:intergenic
CACTCATTGCATTAGGCAAA+AGG 0.405527 3.2:+84842920 None:intergenic
TCAATGCACTACATCCAAAA+AGG 0.406674 3.2:-84844131 MS.gene38361:CDS
CCACGCTGAAGGGATCAAAA+TGG 0.419054 3.2:+84843097 None:intergenic
GTTTATGGCTAACTTGCAAC+TGG 0.422888 3.2:+84843335 None:intergenic
TGAAATTCTTTGAGTGATAG+AGG 0.426454 3.2:+84844235 None:intergenic
GGATATGCAAACAAATTTGA+TGG 0.429813 3.2:-84843000 MS.gene38361:CDS
TGGTTATTTGGTGAAGTTGT+TGG 0.431090 3.2:+84844092 None:intergenic
ATTTGAAGGAGGAGGAAGCA+TGG 0.434087 3.2:-84842956 MS.gene38361:CDS
AGTTTCCAAAGTACTCTTGC+GGG 0.444358 3.2:+84843286 None:intergenic
CCAAAGTACTCTTGCGGGAG+AGG 0.455688 3.2:+84843291 None:intergenic
TGCTTGAGAATGATTTAGGT+TGG 0.465724 3.2:-84843224 MS.gene38361:CDS
CCTCTCCCGCAAGAGTACTT+TGG 0.478058 3.2:-84843291 MS.gene38361:CDS
GCTTGAGAATGATTTAGGTT+GGG 0.480817 3.2:-84843223 MS.gene38361:CDS
ACGATTGGCGGAGCATCAAC+CGG 0.481258 3.2:+84843840 None:intergenic
TCTAGCGCACTCATTGCATT+AGG 0.481834 3.2:+84842913 None:intergenic
GGCCGGAAAGAGGATAGAAA+TGG 0.482201 3.2:+84844009 None:intergenic
AAATTTGTTACTTAACAGGC+TGG 0.483511 3.2:-84844169 MS.gene38361:CDS
TCCTCCTTCAAATCCCTCAT+AGG 0.483907 3.2:+84842966 None:intergenic
TATCGATAAAGCAATCAATC+AGG 0.488699 3.2:-84843871 MS.gene38361:CDS
CGACCAAGTTGGTAGACAAC+CGG 0.489315 3.2:+84843120 None:intergenic
TTGAACGGAAGATTAATTAG+TGG 0.490884 3.2:+84843564 None:intergenic
TAGATTCATCTATGCAACTT+TGG 0.497446 3.2:-84843910 MS.gene38361:CDS
AGGAACCACCGATTATGAAA+AGG 0.505726 3.2:+84843600 None:intergenic
TATTGCAAAACTGAAAGAAA+AGG 0.514598 3.2:-84843463 MS.gene38361:CDS
TTCCCATACATATTGAACCT+TGG 0.519386 3.2:+84843060 None:intergenic
CGGTGGTTCCTAGAAGGTTA+CGG 0.519415 3.2:-84843588 MS.gene38361:CDS
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.2:-84843045 MS.gene38361:CDS
CACTAGCTCAGTGACCTGAA+TGG 0.523158 3.2:+84843755 None:intergenic
ACAGCCTATGAGGGATTTGA+AGG 0.524136 3.2:-84842970 MS.gene38361:CDS
TCAAACAACGAGTCCACGAT+TGG 0.525042 3.2:+84843825 None:intergenic
TGGCAATGTGACAGCCTATG+AGG 0.538642 3.2:-84842980 MS.gene38361:CDS
GAATCTTCAATAGGGTGTAG+TGG 0.541386 3.2:+84844197 None:intergenic
TGCATAGATGAATCTAGCTC+CGG 0.547120 3.2:+84843917 None:intergenic
TTTAGGTTGGGCAGCGTGGA+AGG 0.547420 3.2:-84843211 MS.gene38361:CDS
AAATTGCATTCATAAAATCC+TGG 0.553575 3.2:+84842884 None:intergenic
GTGACAAGACGGCCGGAAAG+AGG 0.554301 3.2:+84843999 None:intergenic
CATTCAGGTCACTGAGCTAG+TGG 0.558225 3.2:-84843754 MS.gene38361:CDS
AATCAATCAGGATGCTCCAC+CGG 0.558802 3.2:-84843859 MS.gene38361:CDS
ATGGAAAAGAGCATTAGAGA+GGG 0.559413 3.2:+84844028 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.2:-84843062 MS.gene38361:CDS
AATGGAAAAGAGCATTAGAG+AGG 0.561847 3.2:+84844027 None:intergenic
AACTCGTCAAAATGTTTGAA+CGG 0.562459 3.2:+84843549 None:intergenic
GGTTGATGCTCCGCCAATCG+TGG 0.563064 3.2:-84843838 MS.gene38361:CDS
TATGAGGGATTTGAAGGAGG+AGG 0.567796 3.2:-84842964 MS.gene38361:CDS
AAATGCCGATAAAGATTGGA+AGG 0.570447 3.2:+84843407 None:intergenic
ACTCATTGCATTAGGCAAAA+GGG 0.570495 3.2:+84842921 None:intergenic
CTTGACATCACCACGCTGAA+GGG 0.571359 3.2:+84843087 None:intergenic
ATGAACCATTGTGTTGGTGA+TGG 0.571518 3.2:-84843708 MS.gene38361:CDS
GATCAAAATGGCGACCAAGT+TGG 0.573234 3.2:+84843109 None:intergenic
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.2:-84843046 MS.gene38361:CDS
TCTCCGGTTGTCTACCAACT+TGG 0.573892 3.2:-84843123 MS.gene38361:CDS
TGAACCATTGTGTTGGTGAT+GGG 0.579011 3.2:-84843707 MS.gene38361:CDS
GATAGAGCAATCAATCATGA+TGG 0.585089 3.2:-84843798 MS.gene38361:CDS
ACTTGACATCACCACGCTGA+AGG 0.585986 3.2:+84843086 None:intergenic
AAGTACTCTTGCGGGAGAGG+TGG 0.588714 3.2:+84843294 None:intergenic
TTTGTGAGTGACAAGACGGC+CGG 0.593267 3.2:+84843992 None:intergenic
CAGGTCACTGAGCTAGTGGA+TGG 0.594154 3.2:-84843750 MS.gene38361:CDS
AATTTGTTACTTAACAGGCT+GGG 0.594863 3.2:-84844168 MS.gene38361:CDS
AACAACGAGTCCACGATTGG+CGG 0.603442 3.2:+84843828 None:intergenic
TGAATTTGGAATGGGAAAAG+CGG 0.603934 3.2:-84843037 MS.gene38361:CDS
AAAGCGGTTGCAGTTCTAAG+TGG 0.606695 3.2:-84843021 MS.gene38361:CDS
CTTTGGAAACTCAGTTGACG+TGG 0.608160 3.2:-84843274 MS.gene38361:CDS
ATGATTTAGGTTGGGCAGCG+TGG 0.611214 3.2:-84843215 MS.gene38361:CDS
ATATATCAAGCTCAAGCCAA+AGG 0.614342 3.2:-84843648 MS.gene38361:CDS
TCAACAAACATGGTATAAGA+GGG 0.616324 3.2:+84843957 None:intergenic
GAGTTTCCAAAGTACTCTTG+CGG 0.617099 3.2:+84843285 None:intergenic
ACAAACATGGTATAAGAGGG+AGG 0.618261 3.2:+84843960 None:intergenic
GTGACCTGAATGGATAGCAA+CGG 0.634165 3.2:+84843765 None:intergenic
GCCTATGAGGGATTTGAAGG+AGG 0.640344 3.2:-84842967 MS.gene38361:CDS
GGCAATGTGACAGCCTATGA+GGG 0.641579 3.2:-84842979 MS.gene38361:CDS
TGTGAATCACGACGATAGAG+AGG 0.645383 3.2:-84843175 MS.gene38361:CDS
ACCACCGATTATGAAAAGGT+TGG 0.648796 3.2:+84843604 None:intergenic
GAGTGCGCTAGAATCAGACC+AGG 0.652006 3.2:-84842902 MS.gene38361:CDS
TGTTCAATGAACCATTGTGT+TGG 0.653933 3.2:-84843714 MS.gene38361:CDS
CAAACATGGTATAAGAGGGA+GGG 0.664234 3.2:+84843961 None:intergenic
AAGTCCCATCACCAACACAA+TGG 0.687793 3.2:+84843703 None:intergenic
ATTGGCGGAGCATCAACCGG+TGG 0.689437 3.2:+84843843 None:intergenic
GTTACTGCAATCAACAAACA+TGG 0.692327 3.2:+84843947 None:intergenic
CTGAATGGATAGCAACGGCA+TGG 0.698801 3.2:+84843770 None:intergenic
GATTGCAGTAACGATAACAC+CGG 0.716514 3.2:-84843936 MS.gene38361:CDS
TGGTGATGTCAAGTTCACCA+AGG 0.737551 3.2:-84843077 MS.gene38361:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTTCTCTCTCAATT+TGG + chr3.2:84843600-84843619 None:intergenic 20.0%
!! AATAATTTCTCTCTCAATTT+GGG + chr3.2:84843599-84843618 None:intergenic 20.0%
!! ATTTGTATTGGATTCTTTAT+TGG + chr3.2:84843663-84843682 None:intergenic 20.0%
!!! GGAAGATATTTTATTTGTAT+TGG + chr3.2:84843675-84843694 None:intergenic 20.0%
!!! TTGTTGGTTTTTTGAATAAT+AGG + chr3.2:84842995-84843014 None:intergenic 20.0%
!!! TTTGGAATTTCTTCAATATA+TGG - chr3.2:84843423-84843442 MS.gene38361:CDS 20.0%
! AAATTGCATTCATAAAATCC+TGG + chr3.2:84844219-84844238 None:intergenic 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.2:84844049-84844068 MS.gene38361:CDS 25.0%
! AGATTTCATTCAGAATCTAT+TGG - chr3.2:84843034-84843053 MS.gene38361:CDS 25.0%
! GATTCTTGATCTATTGTTTA+TGG + chr3.2:84843783-84843802 None:intergenic 25.0%
! TATTGCAAAACTGAAAGAAA+AGG - chr3.2:84843637-84843656 MS.gene38361:CDS 25.0%
!! ATGAAATCTTGTTGGTTATT+TGG + chr3.2:84843023-84843042 None:intergenic 25.0%
!! ATTTGCTTTGAATCTTCAAT+AGG + chr3.2:84842915-84842934 None:intergenic 25.0%
!! TTTGCTTTGAATCTTCAATA+GGG + chr3.2:84842914-84842933 None:intergenic 25.0%
!! TTTTATGAATGCAATTTCTG+TGG - chr3.2:84844222-84844241 MS.gene38361:CDS 25.0%
!! TTTTGAATAATAGGCCTTTT+TGG + chr3.2:84842986-84843005 None:intergenic 25.0%
!!! AAGCAAATTTGTTACTTAAC+AGG - chr3.2:84842927-84842946 MS.gene38361:CDS 25.0%
AAACAAATGCCGATAAAGAT+TGG + chr3.2:84843700-84843719 None:intergenic 30.0%
AACTCGTCAAAATGTTTGAA+CGG + chr3.2:84843554-84843573 None:intergenic 30.0%
AATCTTTATCGGCATTTGTT+TGG - chr3.2:84843699-84843718 MS.gene38361:CDS 30.0%
ATCAACAAACATGGTATAAG+AGG + chr3.2:84843147-84843166 None:intergenic 30.0%
ATCTTCCTTCCAATCTTTAT+CGG - chr3.2:84843688-84843707 MS.gene38361:CDS 30.0%
GAATTGCTTGAGAATGATTT+AGG - chr3.2:84843872-84843891 MS.gene38361:CDS 30.0%
GATTCTGAATGAAATCTTGT+TGG + chr3.2:84843031-84843050 None:intergenic 30.0%
GGATATGCAAACAAATTTGA+TGG - chr3.2:84844100-84844119 MS.gene38361:CDS 30.0%
TAGATTCATCTATGCAACTT+TGG - chr3.2:84843190-84843209 MS.gene38361:CDS 30.0%
TATCGATAAAGCAATCAATC+AGG - chr3.2:84843229-84843248 MS.gene38361:CDS 30.0%
TCAACAAACATGGTATAAGA+GGG + chr3.2:84843146-84843165 None:intergenic 30.0%
TGAAATTCTTTGAGTGATAG+AGG + chr3.2:84842868-84842887 None:intergenic 30.0%
TTGAACGGAAGATTAATTAG+TGG + chr3.2:84843539-84843558 None:intergenic 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.2:84844055-84844074 MS.gene38361:CDS 30.0%
!! AAATTTGTTACTTAACAGGC+TGG - chr3.2:84842931-84842950 MS.gene38361:CDS 30.0%
!! AATTTGTTACTTAACAGGCT+GGG - chr3.2:84842932-84842951 MS.gene38361:CDS 30.0%
AAATGCCGATAAAGATTGGA+AGG + chr3.2:84843696-84843715 None:intergenic 35.0%
ACTCATTGCATTAGGCAAAA+GGG + chr3.2:84844182-84844201 None:intergenic 35.0%
ATATATCAAGCTCAAGCCAA+AGG - chr3.2:84843452-84843471 MS.gene38361:CDS 35.0%
CAAAAATTGGTTAGAGTCTC+CGG - chr3.2:84843961-84843980 MS.gene38361:CDS 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.2:84844038-84844057 MS.gene38361:CDS 35.0%
GATAGAGCAATCAATCATGA+TGG - chr3.2:84843302-84843321 MS.gene38361:CDS 35.0%
GTAAGTACAAAAACGACTGT+AGG - chr3.2:84843848-84843867 MS.gene38361:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.2:84844054-84844073 MS.gene38361:CDS 35.0%
GTTACTGCAATCAACAAACA+TGG + chr3.2:84843156-84843175 None:intergenic 35.0%
TCAATGCACTACATCCAAAA+AGG - chr3.2:84842969-84842988 MS.gene38361:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.2:84844037-84844056 MS.gene38361:CDS 35.0%
TGAGAGAGATGCTCAAAAAT+TGG - chr3.2:84843948-84843967 MS.gene38361:CDS 35.0%
TGCTTGAGAATGATTTAGGT+TGG - chr3.2:84843876-84843895 MS.gene38361:CDS 35.0%
TGTTCAATGAACCATTGTGT+TGG - chr3.2:84843386-84843405 MS.gene38361:CDS 35.0%
TTCCATTTCTATCCTCTTTC+CGG - chr3.2:84843089-84843108 MS.gene38361:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.2:84844043-84844062 None:intergenic 35.0%
! GCTTGAGAATGATTTAGGTT+GGG - chr3.2:84843877-84843896 MS.gene38361:CDS 35.0%
! TGAATTTGGAATGGGAAAAG+CGG - chr3.2:84844063-84844082 MS.gene38361:CDS 35.0%
! TGGTTATTTGGTGAAGTTGT+TGG + chr3.2:84843011-84843030 None:intergenic 35.0%
!! AATGGAAAAGAGCATTAGAG+AGG + chr3.2:84843076-84843095 None:intergenic 35.0%
!! ATGGAAAAGAGCATTAGAGA+GGG + chr3.2:84843075-84843094 None:intergenic 35.0%
AATGAGAACATCATCGCCTT+TGG + chr3.2:84843471-84843490 None:intergenic 40.0%
ACAAACATGGTATAAGAGGG+AGG + chr3.2:84843143-84843162 None:intergenic 40.0%
CAAACATGGTATAAGAGGGA+GGG + chr3.2:84843142-84843161 None:intergenic 40.0%
CACTCATTGCATTAGGCAAA+AGG + chr3.2:84844183-84844202 None:intergenic 40.0%
GAATCTTCAATAGGGTGTAG+TGG + chr3.2:84842906-84842925 None:intergenic 40.0%
GATTGCAGTAACGATAACAC+CGG - chr3.2:84843164-84843183 MS.gene38361:CDS 40.0%
! ACCAACCTTTTCATAATCGG+TGG - chr3.2:84843495-84843514 MS.gene38361:CDS 40.0%
! ACCACCGATTATGAAAAGGT+TGG + chr3.2:84843499-84843518 None:intergenic 40.0%
! AGGAACCACCGATTATGAAA+AGG + chr3.2:84843503-84843522 None:intergenic 40.0%
! AGTTTCCAAAGTACTCTTGC+GGG + chr3.2:84843817-84843836 None:intergenic 40.0%
! ATGAACCATTGTGTTGGTGA+TGG - chr3.2:84843392-84843411 MS.gene38361:CDS 40.0%
! CGCACCAACCTTTTCATAAT+CGG - chr3.2:84843492-84843511 MS.gene38361:CDS 40.0%
! GAGTTTCCAAAGTACTCTTG+CGG + chr3.2:84843818-84843837 None:intergenic 40.0%
! GTTTATGGCTAACTTGCAAC+TGG + chr3.2:84843768-84843787 None:intergenic 40.0%
! TGCATAGATGAATCTAGCTC+CGG + chr3.2:84843186-84843205 None:intergenic 40.0%
!! CTAGTGGATGGTGTTTTCAT+AGG - chr3.2:84843362-84843381 MS.gene38361:CDS 40.0%
!! GAGTTTTGTGAGTGACAAGA+CGG + chr3.2:84843115-84843134 None:intergenic 40.0%
!! TGAACCATTGTGTTGGTGAT+GGG - chr3.2:84843393-84843412 MS.gene38361:CDS 40.0%
!!! TTGGTGATGGGACTTCTTTT+TGG - chr3.2:84843405-84843424 MS.gene38361:CDS 40.0%
AAGTCCCATCACCAACACAA+TGG + chr3.2:84843400-84843419 None:intergenic 45.0%
AATCAATCAGGATGCTCCAC+CGG - chr3.2:84843241-84843260 MS.gene38361:CDS 45.0%
ACAGCCTATGAGGGATTTGA+AGG - chr3.2:84844130-84844149 MS.gene38361:CDS 45.0%
ATTTGAAGGAGGAGGAAGCA+TGG - chr3.2:84844144-84844163 MS.gene38361:CDS 45.0%
CATAATCGGTGGTTCCTAGA+AGG - chr3.2:84843506-84843525 MS.gene38361:CDS 45.0%
GATCAAAATGGCGACCAAGT+TGG + chr3.2:84843994-84844013 None:intergenic 45.0%
GTGACCTGAATGGATAGCAA+CGG + chr3.2:84843338-84843357 None:intergenic 45.0%
TATGAGGGATTTGAAGGAGG+AGG - chr3.2:84844136-84844155 MS.gene38361:CDS 45.0%
TCAAACAACGAGTCCACGAT+TGG + chr3.2:84843278-84843297 None:intergenic 45.0%
TCCTCCTTCAAATCCCTCAT+AGG + chr3.2:84844137-84844156 None:intergenic 45.0%
TCTAGCGCACTCATTGCATT+AGG + chr3.2:84844190-84844209 None:intergenic 45.0%
TGTGAATCACGACGATAGAG+AGG - chr3.2:84843925-84843944 MS.gene38361:CDS 45.0%
! CTTTGGAAACTCAGTTGACG+TGG - chr3.2:84843826-84843845 MS.gene38361:CDS 45.0%
! TGGTGATGTCAAGTTCACCA+AGG - chr3.2:84844023-84844042 MS.gene38361:CDS 45.0%
! TTAGTGGACCGTAACCTTCT+AGG + chr3.2:84843523-84843542 None:intergenic 45.0%
!! AAAGCGGTTGCAGTTCTAAG+TGG - chr3.2:84844079-84844098 MS.gene38361:CDS 45.0%
AACAACGAGTCCACGATTGG+CGG + chr3.2:84843275-84843294 None:intergenic 50.0%
ACTTGACATCACCACGCTGA+AGG + chr3.2:84844017-84844036 None:intergenic 50.0%
CACTAGCTCAGTGACCTGAA+TGG + chr3.2:84843348-84843367 None:intergenic 50.0%
CATGCCGTTGCTATCCATTC+AGG - chr3.2:84843331-84843350 MS.gene38361:CDS 50.0%
CATTCAGGTCACTGAGCTAG+TGG - chr3.2:84843346-84843365 MS.gene38361:CDS 50.0%
CCACGCTGAAGGGATCAAAA+TGG + chr3.2:84844006-84844025 None:intergenic 50.0%
CGGTGGTTCCTAGAAGGTTA+CGG - chr3.2:84843512-84843531 MS.gene38361:CDS 50.0%
CTGAATGGATAGCAACGGCA+TGG + chr3.2:84843333-84843352 None:intergenic 50.0%
CTTGACATCACCACGCTGAA+GGG + chr3.2:84844016-84844035 None:intergenic 50.0%
GCCTATGAGGGATTTGAAGG+AGG - chr3.2:84844133-84844152 MS.gene38361:CDS 50.0%
GGCAATGTGACAGCCTATGA+GGG - chr3.2:84844121-84844140 MS.gene38361:CDS 50.0%
GGCCGGAAAGAGGATAGAAA+TGG + chr3.2:84843094-84843113 None:intergenic 50.0%
TCTCCGGTTGTCTACCAACT+TGG - chr3.2:84843977-84843996 MS.gene38361:CDS 50.0%
TGGCAATGTGACAGCCTATG+AGG - chr3.2:84844120-84844139 MS.gene38361:CDS 50.0%
TTTGTGAGTGACAAGACGGC+CGG + chr3.2:84843111-84843130 None:intergenic 50.0%
! AGGAGGAGGAAGCATGGATT+TGG - chr3.2:84844150-84844169 MS.gene38361:CDS 50.0%
!! ATGATTTAGGTTGGGCAGCG+TGG - chr3.2:84843885-84843904 MS.gene38361:CDS 50.0%
!! CCATTTTGATCCCTTCAGCG+TGG - chr3.2:84844003-84844022 MS.gene38361:CDS 50.0%
!! CGACCAAGTTGGTAGACAAC+CGG + chr3.2:84843983-84844002 None:intergenic 50.0%
AAGTACTCTTGCGGGAGAGG+TGG + chr3.2:84843809-84843828 None:intergenic 55.0%
CAGGTCACTGAGCTAGTGGA+TGG - chr3.2:84843350-84843369 MS.gene38361:CDS 55.0%
GAGTGCGCTAGAATCAGACC+AGG - chr3.2:84844198-84844217 MS.gene38361:CDS 55.0%
! ACGATTGGCGGAGCATCAAC+CGG + chr3.2:84843263-84843282 None:intergenic 55.0%
! CCAAAGTACTCTTGCGGGAG+AGG + chr3.2:84843812-84843831 None:intergenic 55.0%
!! CCTCTCCCGCAAGAGTACTT+TGG - chr3.2:84843809-84843828 MS.gene38361:CDS 55.0%
!! TTTAGGTTGGGCAGCGTGGA+AGG - chr3.2:84843889-84843908 MS.gene38361:CDS 55.0%
GTGACAAGACGGCCGGAAAG+AGG + chr3.2:84843104-84843123 None:intergenic 60.0%
! ATTGGCGGAGCATCAACCGG+TGG + chr3.2:84843260-84843279 None:intergenic 60.0%
! GGTTGATGCTCCGCCAATCG+TGG - chr3.2:84843262-84843281 MS.gene38361:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 84842855 84844267 84842855 ID=MS.gene38361
chr3.2 mRNA 84842855 84844267 84842855 ID=MS.gene38361.t1;Parent=MS.gene38361
chr3.2 exon 84842855 84844267 84842855 ID=MS.gene38361.t1.exon1;Parent=MS.gene38361.t1
chr3.2 CDS 84842855 84844267 84842855 ID=cds.MS.gene38361.t1;Parent=MS.gene38361.t1
Gene Sequence

>MS.gene38361

ATGATGACTTCCTCTATCACTCAAAGAATTTCAGAATGTTATATCAAACCACTACACCCTATTGAAGATTCAAAGCAAATTTGTTACTTAACAGGCTGGGATATTGCTATGTCATCAATGCACTACATCCAAAAAGGCCTATTATTCAAAAAACCAACAACTTCACCAAATAACCAACAAGATTTCATTCAGAATCTATTGGAGAAGCTTAAACACTCCCTCTCTAATGCTCTTTTCCATTTCTATCCTCTTTCCGGCCGTCTTGTCACTCACAAAACTCAAGACCCTCCCTCTTATACCATGTTTGTTGATTGCAGTAACGATAACACCGGAGCTAGATTCATCTATGCAACTTTGGATGCAGCTATATATGATATCGATAAAGCAATCAATCAGGATGCTCCACCGGTTGATGCTCCGCCAATCGTGGACTCGTTGTTTGATCTTGATAGAGCAATCAATCATGATGGTCACACCATGCCGTTGCTATCCATTCAGGTCACTGAGCTAGTGGATGGTGTTTTCATAGGCTGTTCAATGAACCATTGTGTTGGTGATGGGACTTCTTTTTGGAATTTCTTCAATATATGGTCTGAAATATATCAAGCTCAAGCCAAAGGCGATGATGTTCTCATTTCGCACCAACCTTTTCATAATCGGTGGTTCCTAGAAGGTTACGGTCCACTAATTAATCTTCCGTTCAAACATTTTGACGAGTTTACAAGCAGATTTGAAGCAGTACCCAAATTGAGAGAGAAATTATTTCACTTTAGTGCAGAGTCTATTGCAAAACTGAAAGAAAAGGCCAATAAAGAATCCAATACAAATAAAATATCTTCCTTCCAATCTTTATCGGCATTTGTTTGGAGAAGTGTGACTCGTGCACGAAGACTAAAACATGATCAAAAAACCAGTTGCAAGTTAGCCATAAACAATAGATCAAGAATCAAACCACCTCTCCCGCAAGAGTACTTTGGAAACTCAGTTGACGTGGTAAGTACAAAAACGACTGTAGGCGAATTGCTTGAGAATGATTTAGGTTGGGCAGCGTGGAAGGTGCATACACTTGTTGTGAATCACGACGATAGAGAGGTGAGAGAGATGCTCAAAAATTGGTTAGAGTCTCCGGTTGTCTACCAACTTGGTCGCCATTTTGATCCCTTCAGCGTGGTGATGTCAAGTTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGAAAAGCGGTTGCAGTTCTAAGTGGATATGCAAACAAATTTGATGGCAATGTGACAGCCTATGAGGGATTTGAAGGAGGAGGAAGCATGGATTTGGCAGTGTCCCTTTTGCCTAATGCAATGAGTGCGCTAGAATCAGACCAGGATTTTATGAATGCAATTTCTGTGGTCAATTTTTCTAATATTCTGTGA
Protein sequence

>MS.gene38361.t1

MMTSSITQRISECYIKPLHPIEDSKQICYLTGWDIAMSSMHYIQKGLLFKKPTTSPNNQQDFIQNLLEKLKHSLSNALFHFYPLSGRLVTHKTQDPPSYTMFVDCSNDNTGARFIYATLDAAIYDIDKAINQDAPPVDAPPIVDSLFDLDRAINHDGHTMPLLSIQVTELVDGVFIGCSMNHCVGDGTSFWNFFNIWSEIYQAQAKGDDVLISHQPFHNRWFLEGYGPLINLPFKHFDEFTSRFEAVPKLREKLFHFSAESIAKLKEKANKESNTNKISSFQSLSAFVWRSVTRARRLKHDQKTSCKLAINNRSRIKPPLPQEYFGNSVDVVSTKTTVGELLENDLGWAAWKVHTLVVNHDDREVREMLKNWLESPVVYQLGRHFDPFSVVMSSSPRFNMYGNEFGMGKAVAVLSGYANKFDGNVTAYEGFEGGGSMDLAVSLLPNAMSALESDQDFMNAISVVNFSNIL