AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039597

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039597.t1 MTR_3g101970 90.929 452 26 2 1 451 1 438 0.0 852
MS.gene039597.t1 MTR_3g101960 88.315 445 52 0 1 445 1 445 0.0 833
MS.gene039597.t1 MTR_3g102020 85.120 457 67 1 1 456 1 457 0.0 816
MS.gene039597.t1 MTR_3g102010 79.565 460 86 2 1 456 2 457 0.0 758
MS.gene039597.t1 MTR_3g101900 67.253 455 138 4 1 451 2 449 0.0 619
MS.gene039597.t1 MTR_3g101930 66.000 450 139 5 9 449 10 454 0.0 602
MS.gene039597.t1 MTR_3g102030 72.956 318 60 2 86 403 29 320 1.21e-168 477
MS.gene039597.t1 MTR_4g015180 39.286 504 235 11 1 450 1 487 6.03e-98 303
MS.gene039597.t1 MTR_3g070170 38.066 486 227 13 5 446 11 466 2.00e-96 298
MS.gene039597.t1 MTR_5g081960 38.523 501 234 12 1 446 1 482 2.22e-96 298
MS.gene039597.t1 MTR_4g094842 39.301 458 250 11 3 446 1 444 2.71e-93 289
MS.gene039597.t1 MTR_4g022825 38.688 442 244 9 20 450 15 440 1.95e-89 279
MS.gene039597.t1 MTR_8g041770 39.529 425 241 9 29 450 30 441 3.94e-89 278
MS.gene039597.t1 MTR_4g022800 38.149 443 245 11 21 450 16 442 2.46e-88 276
MS.gene039597.t1 MTR_0190s0020 38.642 427 246 8 28 450 28 442 5.51e-88 275
MS.gene039597.t1 MTR_4g094835 36.980 457 260 10 3 446 1 442 4.92e-86 271
MS.gene039597.t1 MTR_4g022720 39.080 435 245 9 21 450 16 435 7.08e-86 270
MS.gene039597.t1 MTR_3g088420 38.621 435 241 10 23 450 18 433 3.56e-85 268
MS.gene039597.t1 MTR_4g022670 37.868 441 248 9 21 450 16 441 6.04e-85 268
MS.gene039597.t1 MTR_7g026860 36.961 441 249 10 21 450 16 438 1.09e-83 264
MS.gene039597.t1 MTR_4g094838 37.150 428 234 8 28 446 27 428 4.91e-82 260
MS.gene039597.t1 MTR_3g026310 38.182 440 239 11 29 450 24 448 1.97e-81 259
MS.gene039597.t1 MTR_4g094828 36.910 466 252 15 8 451 6 451 1.08e-80 257
MS.gene039597.t1 MTR_4g022290 36.429 420 235 10 23 429 24 424 6.53e-78 249
MS.gene039597.t1 MTR_8g079710 36.830 429 230 14 21 431 21 426 3.37e-72 234
MS.gene039597.t1 MTR_4g094832 38.968 349 198 6 105 446 86 426 3.46e-69 226
MS.gene039597.t1 MTR_4g022760 32.880 441 226 10 21 450 16 397 9.27e-65 214
MS.gene039597.t1 MTR_6g045553 31.405 484 271 12 5 449 13 474 2.44e-61 207
MS.gene039597.t1 MTR_6g016815 31.163 430 214 12 23 450 32 381 2.11e-48 170
MS.gene039597.t1 MTR_8g040780 38.073 218 130 4 240 453 20 236 4.93e-41 147
MS.gene039597.t1 MTR_4g007540 26.269 453 288 16 6 440 3 427 2.89e-34 133
MS.gene039597.t1 MTR_8g075610 28.607 402 224 15 68 440 47 414 9.39e-33 129
MS.gene039597.t1 MTR_2g064495 26.536 407 271 13 48 440 34 426 4.14e-31 124
MS.gene039597.t1 MTR_2g105330 26.290 407 272 13 48 440 34 426 1.28e-30 123
MS.gene039597.t1 MTR_8g040760 36.406 217 127 6 8 223 6 212 6.41e-30 116
MS.gene039597.t1 MTR_7g026850 43.333 150 74 4 42 190 20 159 8.79e-30 114
MS.gene039597.t1 MTR_8g096530 29.841 315 190 11 62 362 53 350 2.46e-28 117
MS.gene039597.t1 MTR_7g026770 37.640 178 90 3 155 332 132 288 5.46e-27 110
MS.gene039597.t1 MTR_3g098980 29.967 307 187 12 68 362 66 356 1.89e-26 111
MS.gene039597.t1 MTR_4g127650 26.742 445 260 17 19 431 30 440 2.00e-26 111
MS.gene039597.t1 MTR_3g049150 27.361 413 234 17 56 431 46 429 4.81e-26 110
MS.gene039597.t1 MTR_6g034495 44.915 118 61 3 79 193 45 161 4.60e-24 98.6
MS.gene039597.t1 MTR_4g094825 38.667 150 81 2 301 450 253 391 7.58e-24 103
MS.gene039597.t1 MTR_4g094825 33.846 195 112 6 29 220 92 272 1.23e-21 96.7
MS.gene039597.t1 MTR_1g040175 28.883 367 224 17 65 419 61 402 9.65e-24 103
MS.gene039597.t1 MTR_6g053810 31.488 289 174 12 69 344 72 349 1.01e-23 103
MS.gene039597.t1 MTR_4g097090 24.554 448 274 16 29 440 30 449 7.45e-23 101
MS.gene039597.t1 MTR_3g026950 25.432 405 248 15 62 431 48 433 4.01e-22 99.0
MS.gene039597.t1 MTR_3g112180 27.666 347 225 11 65 399 58 390 4.96e-22 98.6
MS.gene039597.t1 MTR_5g076700 24.837 459 291 17 2 436 3 431 6.84e-22 98.2
MS.gene039597.t1 MTR_1g040195 24.762 420 277 16 50 450 21 420 1.25e-20 94.4
MS.gene039597.t1 MTR_7g027175 28.840 319 200 11 56 362 56 359 5.69e-20 92.4
MS.gene039597.t1 MTR_1g081660 26.923 390 246 15 65 437 70 437 8.30e-20 92.0
MS.gene039597.t1 MTR_7g114550 25.727 447 276 20 30 447 24 443 1.48e-19 91.3
MS.gene039597.t1 MTR_3g465980 26.149 348 215 13 6 334 10 334 3.06e-19 90.1
MS.gene039597.t1 MTR_7g114520 26.151 478 288 19 2 448 4 447 3.43e-19 90.1
MS.gene039597.t1 MTR_1g040215 27.011 348 217 15 13 347 16 339 3.67e-19 90.1
MS.gene039597.t1 MTR_7g086000 27.020 396 241 19 71 433 63 443 7.85e-19 89.0
MS.gene039597.t1 MTR_7g014160 25.214 468 293 20 15 441 16 467 1.89e-18 87.8
MS.gene039597.t1 MTR_6g034485 33.065 124 82 1 205 327 2 125 1.38e-17 79.3
MS.gene039597.t1 MTR_3g465990 25.723 346 219 13 6 334 10 334 1.76e-17 85.1
MS.gene039597.t1 MTR_4g055170 24.911 281 184 8 68 334 69 336 2.14e-17 84.7
MS.gene039597.t1 MTR_1g040315 27.362 307 196 11 30 325 23 313 2.23e-17 84.3
MS.gene039597.t1 MTR_7g114960 25.758 396 269 10 65 446 60 444 2.57e-17 84.3
MS.gene039597.t1 MTR_7g084940 25.524 286 183 10 64 334 65 335 9.22e-17 82.8
MS.gene039597.t1 MTR_6g015320 25.553 407 252 15 60 436 58 443 2.29e-16 81.6
MS.gene039597.t1 MTR_7g013850 24.823 423 252 16 30 402 30 436 3.98e-16 80.9
MS.gene039597.t1 MTR_4g013050 24.688 401 220 16 66 436 51 399 6.26e-16 79.7
MS.gene039597.t1 MTR_3g465930 27.338 278 176 9 68 331 69 334 6.43e-16 80.1
MS.gene039597.t1 MTR_7g026830 50.000 70 34 1 111 179 28 97 7.84e-15 70.5
MS.gene039597.t1 MTR_3g049140 26.108 203 134 4 242 431 27 226 9.75e-15 74.3
MS.gene039597.t1 MTR_2g015430 24.638 345 227 13 71 401 61 386 1.84e-14 75.5
MS.gene039597.t1 MTR_7g085930 23.333 420 265 15 50 431 45 445 5.70e-14 74.3
MS.gene039597.t1 MTR_3g465910 26.316 285 166 11 69 331 69 331 9.31e-14 73.6
MS.gene039597.t1 MTR_3g465950 26.882 279 175 8 69 331 69 334 3.06e-13 72.0
MS.gene039597.t1 MTR_5g090830 22.997 387 240 17 48 399 49 412 4.06e-13 71.6
MS.gene039597.t1 MTR_7g014620 23.821 424 282 18 29 432 15 417 4.72e-13 71.2
MS.gene039597.t1 MTR_2g045540 35.227 88 49 1 242 329 27 106 5.98e-13 65.5
MS.gene039597.t1 MTR_5g090810 22.312 372 234 15 63 399 61 412 1.78e-12 69.3
MS.gene039597.t1 MTR_0502s0010 29.200 250 133 12 111 337 2 230 2.02e-12 67.4
MS.gene039597.t1 MTR_6g015315 25.000 416 260 14 55 439 50 444 2.56e-12 68.9
MS.gene039597.t1 MTR_7g014610 22.494 449 294 17 29 436 29 464 2.77e-12 68.9
MS.gene039597.t1 MTR_5g090880 23.784 370 230 17 64 399 63 414 4.23e-12 68.2
MS.gene039597.t1 MTR_7g084910 23.765 324 206 11 18 315 10 318 1.25e-11 66.6
MS.gene039597.t1 MTR_7g056087 22.427 379 248 14 6 361 4 359 1.41e-11 66.6
MS.gene039597.t1 MTR_7g014630 30.233 215 136 7 2 209 3 210 2.26e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039597.t1 AT5G42830 56.054 446 180 4 13 451 14 450 0.0 513
MS.gene039597.t1 AT5G07850 53.363 446 207 1 6 450 8 453 2.13e-172 492
MS.gene039597.t1 AT5G07860 53.125 448 199 5 6 449 8 448 1.68e-162 466
MS.gene039597.t1 AT5G07870 50.437 458 210 3 6 450 8 461 1.33e-159 459
MS.gene039597.t1 AT5G67150 39.868 454 250 12 8 451 7 447 1.67e-101 310
MS.gene039597.t1 AT2G39980 40.127 471 214 10 29 450 29 480 3.58e-100 308
MS.gene039597.t1 AT5G01210 38.525 488 241 12 3 450 1 469 5.83e-90 281
MS.gene039597.t1 AT3G50270 36.404 456 264 12 8 451 8 449 3.12e-84 266
MS.gene039597.t1 AT3G50280 35.841 452 269 11 3 446 1 439 9.13e-80 254
MS.gene039597.t1 AT3G50300 35.780 436 257 12 6 432 4 425 5.47e-77 247
MS.gene039597.t1 AT5G38130 35.308 439 253 12 6 432 24 443 1.23e-71 234
MS.gene039597.t1 AT5G67160 35.280 428 251 11 28 451 28 433 4.26e-70 229
MS.gene039597.t1 AT5G23940 33.696 460 276 12 6 448 3 450 1.00e-65 219
MS.gene039597.t1 AT3G50280 38.762 307 177 6 146 446 8 309 2.24e-56 189
MS.gene039597.t1 AT3G50290 37.452 259 149 6 147 396 15 269 5.47e-43 153
MS.gene039597.t1 AT5G48930 26.535 456 283 16 6 440 3 427 7.49e-34 132
MS.gene039597.t1 AT2G19070 27.982 436 256 16 29 431 25 435 4.24e-33 130
MS.gene039597.t1 AT5G41040 29.661 354 212 13 62 399 63 395 1.30e-28 117
MS.gene039597.t1 AT5G41040 29.661 354 212 13 62 399 79 411 1.90e-28 117
MS.gene039597.t1 AT3G48720 29.341 334 193 15 18 334 18 325 1.54e-23 102
MS.gene039597.t1 AT1G31490 28.162 419 245 21 58 455 52 435 1.52e-21 97.1
MS.gene039597.t1 AT5G63560 28.344 314 190 12 62 361 55 347 1.03e-20 94.4
MS.gene039597.t1 AT1G65450 28.477 302 177 12 56 335 60 344 1.19e-20 94.4
MS.gene039597.t1 AT2G40230 26.190 378 214 14 63 409 54 397 6.72e-19 89.0
MS.gene039597.t1 AT1G28680 26.280 293 193 12 71 353 65 344 1.46e-18 87.8
MS.gene039597.t1 AT1G28680 26.280 293 193 12 71 353 65 344 2.13e-18 87.8
MS.gene039597.t1 AT3G26040 25.470 479 278 23 6 454 3 432 3.33e-17 84.0
MS.gene039597.t1 AT1G27620 27.129 317 179 10 68 358 58 348 1.20e-16 82.4
MS.gene039597.t1 AT1G78990 23.625 309 195 12 48 342 52 333 1.95e-16 81.6
MS.gene039597.t1 AT5G16410 23.981 417 259 16 66 455 89 474 1.32e-15 79.3
MS.gene039597.t1 AT3G03480 25.915 328 211 13 30 342 33 343 3.50e-15 77.8
MS.gene039597.t1 AT1G03390 24.570 407 244 18 56 431 67 441 1.04e-14 76.6
MS.gene039597.t1 AT2G25150 26.364 330 209 14 61 374 60 371 2.44e-14 75.5
MS.gene039597.t1 AT1G65450 28.723 188 108 6 166 335 1 180 1.01e-13 72.0
MS.gene039597.t1 AT5G39080 21.712 479 306 17 3 439 2 453 6.86e-11 64.7

Find 106 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.1:-9120140 MS.gene039597:CDS
CAAAATGATATTCCTGTTTA+TGG 0.111562 3.1:-9120485 MS.gene039597:CDS
AATACGTATGGAAATGAATT+TGG 0.183687 3.1:-9119900 MS.gene039597:CDS
TTAAATGGAAGGTTGATAAT+TGG 0.249266 3.1:+9120410 None:intergenic
AACTCGTGGTGATGTTTAAA+TGG 0.296206 3.1:+9120395 None:intergenic
AATCTTTATCAGCATTAGTT+TGG 0.307683 3.1:-9120250 MS.gene039597:CDS
CCACTTATCATCACAGTATA+AGG 0.318537 3.1:+9119936 None:intergenic
CACGAGTTTGTTCACACATA+TGG 0.341216 3.1:-9120380 MS.gene039597:CDS
AATGTGTTAAATGACATATT+TGG 0.351013 3.1:+9120987 None:intergenic
ATAATTGGACCATAACCTTC+TGG 0.355852 3.1:+9120425 None:intergenic
TCATGCACGTCGACTACAAC+AGG 0.363744 3.1:-9120216 MS.gene039597:CDS
AACATGCAGAAACGATGTTT+AGG 0.378660 3.1:+9121044 None:intergenic
ACAATGGGTGGGACATCAAT+GGG 0.389297 3.1:+9120683 None:intergenic
TCTGTGATGGAACATCTTAT+TGG 0.391840 3.1:-9120538 MS.gene039597:CDS
GGATATGCTAACAAATTTGA+TGG 0.406437 3.1:-9119849 MS.gene039597:CDS
CTCCCTCCTCCTTCTTGACC+TGG 0.408040 3.1:+9119810 None:intergenic
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.1:-9120434 MS.gene039597:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.415536 3.1:-9120830 MS.gene039597:CDS
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.1:+9120460 None:intergenic
TCAGAGAACAACTTGCAAAA+TGG 0.421540 3.1:-9120192 MS.gene039597:CDS
ATCTACACATTATAACCAAA+AGG 0.424756 3.1:-9120948 MS.gene039597:CDS
ATTTGGAATGGGAAAAGCTT+TGG 0.427618 3.1:-9119883 MS.gene039597:CDS
AATATGTCATTTAACACATT+GGG 0.429631 3.1:-9120984 MS.gene039597:CDS
ACATGCAGAAACGATGTTTA+GGG 0.431483 3.1:+9121045 None:intergenic
ATCAACCTATCCACAATAGA+TGG 0.431811 3.1:-9120451 MS.gene039597:CDS
ATTTGGTTTGAGTCTTCGAT+TGG 0.434962 3.1:+9121004 None:intergenic
TGTGAAATGGGACCATAAAC+AGG 0.441469 3.1:+9120473 None:intergenic
ATCACAGAGACAATGATTCA+TGG 0.442497 3.1:+9120553 None:intergenic
TTTGGTTTGAGTCTTCGATT+GGG 0.443458 3.1:+9121005 None:intergenic
TGCATGAATAAATTTAGCTC+CGG 0.444458 3.1:+9120739 None:intergenic
AATGGAAAAGCGTGAGGGAA+AGG 0.449937 3.1:+9120846 None:intergenic
AGTATAAGAATGAGGATCTT+CGG 0.458409 3.1:+9120787 None:intergenic
TATGGAAATGAATTTGGAAT+GGG 0.460328 3.1:-9119894 MS.gene039597:CDS
ATGGAAAAGCGTGAGGGAAA+GGG 0.461186 3.1:+9120847 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.465501 3.1:+9120891 None:intergenic
GACAATGGGTGGGACATCAA+TGG 0.465845 3.1:+9120682 None:intergenic
TCAAAGAACGATTGGACAAT+GGG 0.466912 3.1:+9120668 None:intergenic
GAGGTGGTTCCATTCTTGTT+CGG 0.467336 3.1:+9120159 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.1:+9120140 None:intergenic
ATGATAATAAAGTGGTGCTT+AGG 0.471109 3.1:-9120016 MS.gene039597:CDS
GAGTTGCTAGAGAATGATCT+AGG 0.474371 3.1:-9120077 MS.gene039597:CDS
CAAGTCACAGAATTAGTAGA+TGG 0.474789 3.1:-9120593 MS.gene039597:CDS
GAATTCCCAAAATATTCCTT+AGG 0.477642 3.1:+9120134 None:intergenic
GGAAAAGTAATTTCATATCC+AGG 0.482689 3.1:-9119828 MS.gene039597:CDS
TCACCGAGGTTTAATACGTA+TGG 0.489403 3.1:-9119912 MS.gene039597:CDS
AAATATGTCATTTAACACAT+TGG 0.493987 3.1:-9120985 MS.gene039597:CDS
AGTGTTTCAGTTTGTCCACC+AGG 0.507243 3.1:+9120870 None:intergenic
TGTTGGTTTAGTAATGATGT+TGG 0.510991 3.1:+9120908 None:intergenic
CAATGGGTGGGACATCAATG+GGG 0.513002 3.1:+9120684 None:intergenic
TGCTAGAGAATGATCTAGGA+TGG 0.520529 3.1:-9120073 MS.gene039597:CDS
TGCAAATCATGATAATAAAG+TGG 0.522035 3.1:-9120024 MS.gene039597:CDS
ACCTGAGAAAATGATGGTGC+TGG 0.523005 3.1:-9119763 MS.gene039597:CDS
TACAAACAACCGAACAAGAA+TGG 0.524034 3.1:-9120168 MS.gene039597:CDS
TGTCATGATCAAAGAACGAT+TGG 0.527909 3.1:+9120660 None:intergenic
GCATGAATAAATTTAGCTCC+GGG 0.529301 3.1:+9120740 None:intergenic
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.1:+9120461 None:intergenic
GTATGGAAATGAATTTGGAA+TGG 0.534748 3.1:-9119895 MS.gene039597:CDS
CCTTATACTGTGATGATAAG+TGG 0.535234 3.1:-9119936 MS.gene039597:CDS
AATGGAACCACCTCTTCCTA+AGG 0.542226 3.1:-9120150 MS.gene039597:CDS
TGTAGTGAGTGCTGAAACGA+CGG 0.546049 3.1:-9120105 MS.gene039597:CDS
TCTACACATTATAACCAAAA+GGG 0.546861 3.1:-9120947 MS.gene039597:CDS
GGAAACCATCTATTGTGGAT+AGG 0.548035 3.1:+9120446 None:intergenic
CGTGGTGATGTTTAAATGGA+AGG 0.548108 3.1:+9120399 None:intergenic
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.1:+9120817 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.1:+9120828 None:intergenic
AAAGCTTTGGCAGTTAGAAG+TGG 0.567341 3.1:-9119870 MS.gene039597:CDS
AATGGGTGGGACATCAATGG+GGG 0.568371 3.1:+9120685 None:intergenic
TTGGTTTGAGTCTTCGATTG+GGG 0.568932 3.1:+9121006 None:intergenic
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.1:-9120064 MS.gene039597:CDS
ATCAAAGAACGATTGGACAA+TGG 0.570569 3.1:+9120667 None:intergenic
ACGAAAACAGTATAAGAATG+AGG 0.571532 3.1:+9120779 None:intergenic
GACAAAGCAGTCAACCATGA+TGG 0.574887 3.1:-9120641 MS.gene039597:CDS
ATGAATCATTGTCTCTGTGA+TGG 0.575359 3.1:-9120551 MS.gene039597:CDS
TGCTTAGGTCGGTTGAAGAG+TGG 0.577268 3.1:-9120001 MS.gene039597:CDS
TATAGCGAAACTTAAAGCAA+AGG 0.578915 3.1:-9120312 MS.gene039597:CDS
TATCCAGGTCAAGAAGGAGG+AGG 0.584517 3.1:-9119813 MS.gene039597:CDS
CACAATAGATGGTTTCCAGA+AGG 0.584706 3.1:-9120440 MS.gene039597:CDS
ATTTCATATCCAGGTCAAGA+AGG 0.586316 3.1:-9119819 MS.gene039597:CDS
CAGAAACGATGTTTAGGGAA+GGG 0.587217 3.1:+9121050 None:intergenic
GTTATAATGTGTAGATAACA+TGG 0.589863 3.1:+9120955 None:intergenic
ATAGTCAAAGATTAGAACAA+AGG 0.594150 3.1:+9119699 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.1:+9120143 None:intergenic
CTTCTGGAAACCATCTATTG+TGG 0.595445 3.1:+9120441 None:intergenic
ATCCAGGTCAAGAAGGAGGA+GGG 0.602246 3.1:-9119812 MS.gene039597:CDS
GCAGAAACGATGTTTAGGGA+AGG 0.605789 3.1:+9121049 None:intergenic
TTTCCATACGTATTAAACCT+CGG 0.609189 3.1:+9119909 None:intergenic
ACAACATTTCATAGAGAACC+TGG 0.610615 3.1:-9120888 MS.gene039597:CDS
AAGAACGATTGGACAATGGG+TGG 0.614907 3.1:+9120671 None:intergenic
AGAACGATTGGACAATGGGT+GGG 0.617288 3.1:+9120672 None:intergenic
TAATAAAGTGGTGCTTAGGT+CGG 0.624945 3.1:-9120012 MS.gene039597:CDS
GCTAGAGAATGATCTAGGAT+GGG 0.627088 3.1:-9120072 MS.gene039597:CDS
TGGTTTGAGTCTTCGATTGG+GGG 0.638810 3.1:+9121007 None:intergenic
GATAGAAATGGAAAAGCGTG+AGG 0.641029 3.1:+9120840 None:intergenic
TCTTTCACCTGAGAAAATGA+TGG 0.654677 3.1:-9119769 MS.gene039597:CDS
TCATATCCAGGTCAAGAAGG+AGG 0.656740 3.1:-9119816 MS.gene039597:CDS
AAGACATGGTGTGACCATCA+TGG 0.667161 3.1:+9120627 None:intergenic
GGCAGCATGGAAAATTCATG+TGG 0.671300 3.1:-9120051 MS.gene039597:CDS
GTGAGTGCTGAAACGACGGT+AGG 0.677302 3.1:-9120101 MS.gene039597:CDS
ATAGAAATGGAAAAGCGTGA+GGG 0.687050 3.1:+9120841 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.1:+9120816 None:intergenic
CATGCACGTCGACTACAACA+GGG 0.700954 3.1:-9120215 MS.gene039597:CDS
TTGAATAGATAACAAAGACA+TGG 0.705301 3.1:+9120613 None:intergenic
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.1:+9120818 None:intergenic
ACATTTCATAGAGAACCTGG+TGG 0.735789 3.1:-9120885 MS.gene039597:CDS
TGATGATAAGTGGTTCACCG+AGG 0.741768 3.1:-9119926 MS.gene039597:CDS
CATATGTGTGAACAAACTCG+TGG 0.754058 3.1:+9120381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTAATCGATACAAAAA+AGG + chr3.1:9120802-9120821 None:intergenic 20.0%
!! AATGTGTTAAATGACATATT+TGG + chr3.1:9119784-9119803 None:intergenic 20.0%
!!! AAATATGTCATTTAACACAT+TGG - chr3.1:9119783-9119802 MS.gene039597:CDS 20.0%
!!! AAGATTCTCTCTTTTAAAAT+GGG + chr3.1:9120418-9120437 None:intergenic 20.0%
!!! AATATGTCATTTAACACATT+GGG - chr3.1:9119784-9119803 MS.gene039597:CDS 20.0%
! AATACGTATGGAAATGAATT+TGG - chr3.1:9120868-9120887 MS.gene039597:CDS 25.0%
! AATCTTTATCAGCATTAGTT+TGG - chr3.1:9120518-9120537 MS.gene039597:CDS 25.0%
! ATCTACACATTATAACCAAA+AGG - chr3.1:9119820-9119839 MS.gene039597:CDS 25.0%
! CAAAATGATATTCCTGTTTA+TGG - chr3.1:9120283-9120302 MS.gene039597:CDS 25.0%
! GTTATAATGTGTAGATAACA+TGG + chr3.1:9119816-9119835 None:intergenic 25.0%
! TCTACACATTATAACCAAAA+GGG - chr3.1:9119821-9119840 MS.gene039597:CDS 25.0%
! TGCAAATCATGATAATAAAG+TGG - chr3.1:9120744-9120763 MS.gene039597:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.1:9120158-9120177 None:intergenic 25.0%
!! TATGGAAATGAATTTGGAAT+GGG - chr3.1:9120874-9120893 MS.gene039597:CDS 25.0%
!!! GAAGATTCTCTCTTTTAAAA+TGG + chr3.1:9120419-9120438 None:intergenic 25.0%
!!! TTAAATGGAAGGTTGATAAT+TGG + chr3.1:9120361-9120380 None:intergenic 25.0%
ACGAAAACAGTATAAGAATG+AGG + chr3.1:9119992-9120011 None:intergenic 30.0%
AGTATAAGAATGAGGATCTT+CGG + chr3.1:9119984-9120003 None:intergenic 30.0%
ATGATAATAAAGTGGTGCTT+AGG - chr3.1:9120752-9120771 MS.gene039597:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.1:9120637-9120656 None:intergenic 30.0%
GGAAAAGTAATTTCATATCC+AGG - chr3.1:9120940-9120959 MS.gene039597:CDS 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.1:9120919-9120938 MS.gene039597:CDS 30.0%
GTATGGAAATGAATTTGGAA+TGG - chr3.1:9120873-9120892 MS.gene039597:CDS 30.0%
TATAGCGAAACTTAAAGCAA+AGG - chr3.1:9120456-9120475 MS.gene039597:CDS 30.0%
TTTCCATACGTATTAAACCT+CGG + chr3.1:9120862-9120881 None:intergenic 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.1:9120629-9120648 MS.gene039597:CDS 30.0%
! TGCATGAATAAATTTAGCTC+CGG + chr3.1:9120032-9120051 None:intergenic 30.0%
! TTAGTAGATGGTGTTTTCAT+TGG - chr3.1:9120187-9120206 MS.gene039597:CDS 30.0%
!! TGTTGGTTTAGTAATGATGT+TGG + chr3.1:9119863-9119882 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.1:9120628-9120647 None:intergenic 35.0%
AACATGCAGAAACGATGTTT+AGG + chr3.1:9119727-9119746 None:intergenic 35.0%
ACAACATTTCATAGAGAACC+TGG - chr3.1:9119880-9119899 MS.gene039597:CDS 35.0%
ACATGCAGAAACGATGTTTA+GGG + chr3.1:9119726-9119745 None:intergenic 35.0%
ATAATTGGACCATAACCTTC+TGG + chr3.1:9120346-9120365 None:intergenic 35.0%
ATCAAAGAACGATTGGACAA+TGG + chr3.1:9120104-9120123 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.1:9120317-9120336 MS.gene039597:CDS 35.0%
ATCACAGAGACAATGATTCA+TGG + chr3.1:9120218-9120237 None:intergenic 35.0%
ATGAATCATTGTCTCTGTGA+TGG - chr3.1:9120217-9120236 MS.gene039597:CDS 35.0%
ATTTCATATCCAGGTCAAGA+AGG - chr3.1:9120949-9120968 MS.gene039597:CDS 35.0%
CAAGTCACAGAATTAGTAGA+TGG - chr3.1:9120175-9120194 MS.gene039597:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.1:9120631-9120650 None:intergenic 35.0%
CCACTTATCATCACAGTATA+AGG + chr3.1:9120835-9120854 None:intergenic 35.0%
CCTTATACTGTGATGATAAG+TGG - chr3.1:9120832-9120851 MS.gene039597:CDS 35.0%
CTATAGATTCCGCTGAAAAA+TGG + chr3.1:9120441-9120460 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.1:9119880-9119899 None:intergenic 35.0%
TAATAAAGTGGTGCTTAGGT+CGG - chr3.1:9120756-9120775 MS.gene039597:CDS 35.0%
TACAAACAACCGAACAAGAA+TGG - chr3.1:9120600-9120619 MS.gene039597:CDS 35.0%
TCAAAGAACGATTGGACAAT+GGG + chr3.1:9120103-9120122 None:intergenic 35.0%
TCAGAGAACAACTTGCAAAA+TGG - chr3.1:9120576-9120595 MS.gene039597:CDS 35.0%
TCTTTCACCTGAGAAAATGA+TGG - chr3.1:9120999-9121018 MS.gene039597:CDS 35.0%
TGTCATGATCAAAGAACGAT+TGG + chr3.1:9120111-9120130 None:intergenic 35.0%
! AACTCGTGGTGATGTTTAAA+TGG + chr3.1:9120376-9120395 None:intergenic 35.0%
! ATAGAAATGGAAAAGCGTGA+GGG + chr3.1:9119930-9119949 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCTT+TGG - chr3.1:9120885-9120904 MS.gene039597:CDS 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.1:9120628-9120647 MS.gene039597:CDS 35.0%
! GCATGAATAAATTTAGCTCC+GGG + chr3.1:9120031-9120050 None:intergenic 35.0%
! TCTGTGATGGAACATCTTAT+TGG - chr3.1:9120230-9120249 MS.gene039597:CDS 35.0%
!! ATTTGGTTTGAGTCTTCGAT+TGG + chr3.1:9119767-9119786 None:intergenic 35.0%
!! GAGAATCTTCCATTTTTCAG+CGG - chr3.1:9120429-9120448 MS.gene039597:CDS 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.1:9120310-9120329 None:intergenic 35.0%
!! TTTGGTTTGAGTCTTCGATT+GGG + chr3.1:9119766-9119785 None:intergenic 35.0%
!! TTTTGAAGAGAAGTCCCTTT+TGG + chr3.1:9119838-9119857 None:intergenic 35.0%
ACATTTCATAGAGAACCTGG+TGG - chr3.1:9119883-9119902 MS.gene039597:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.1:9120334-9120353 MS.gene039597:CDS 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.1:9120328-9120347 MS.gene039597:CDS 40.0%
CAGAAACGATGTTTAGGGAA+GGG + chr3.1:9119721-9119740 None:intergenic 40.0%
CATATGTGTGAACAAACTCG+TGG + chr3.1:9120390-9120409 None:intergenic 40.0%
GAGTTGCTAGAGAATGATCT+AGG - chr3.1:9120691-9120710 MS.gene039597:CDS 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.1:9120325-9120344 None:intergenic 40.0%
TCACCGAGGTTTAATACGTA+TGG - chr3.1:9120856-9120875 MS.gene039597:CDS 40.0%
TGCTAGAGAATGATCTAGGA+TGG - chr3.1:9120695-9120714 MS.gene039597:CDS 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.1:9120298-9120317 None:intergenic 40.0%
! AAAGCTTTGGCAGTTAGAAG+TGG - chr3.1:9120898-9120917 MS.gene039597:CDS 40.0%
! CACGAGTTTGTTCACACATA+TGG - chr3.1:9120388-9120407 MS.gene039597:CDS 40.0%
! CGTGGTGATGTTTAAATGGA+AGG + chr3.1:9120372-9120391 None:intergenic 40.0%
! CTTCTGGAAACCATCTATTG+TGG + chr3.1:9120330-9120349 None:intergenic 40.0%
! GATAGAAATGGAAAAGCGTG+AGG + chr3.1:9119931-9119950 None:intergenic 40.0%
! GCTAGAGAATGATCTAGGAT+GGG - chr3.1:9120696-9120715 MS.gene039597:CDS 40.0%
! TTGGTTTGAGTCTTCGATTG+GGG + chr3.1:9119765-9119784 None:intergenic 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.1:9120311-9120330 None:intergenic 40.0%
AAGAACGATTGGACAATGGG+TGG + chr3.1:9120100-9120119 None:intergenic 45.0%
AAGACATGGTGTGACCATCA+TGG + chr3.1:9120144-9120163 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.1:9120618-9120637 MS.gene039597:CDS 45.0%
ACAATGGGTGGGACATCAAT+GGG + chr3.1:9120088-9120107 None:intergenic 45.0%
AGAACGATTGGACAATGGGT+GGG + chr3.1:9120099-9120118 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.1:9119954-9119973 None:intergenic 45.0%
AGTGTTTCAGTTTGTCCACC+AGG + chr3.1:9119901-9119920 None:intergenic 45.0%
GACAAAGCAGTCAACCATGA+TGG - chr3.1:9120127-9120146 MS.gene039597:CDS 45.0%
GAGGTGGTTCCATTCTTGTT+CGG + chr3.1:9120612-9120631 None:intergenic 45.0%
GCAGAAACGATGTTTAGGGA+AGG + chr3.1:9119722-9119741 None:intergenic 45.0%
GGCAGCATGGAAAATTCATG+TGG - chr3.1:9120717-9120736 MS.gene039597:CDS 45.0%
GGGGGTGAGGATATCATTTA+TGG + chr3.1:9120068-9120087 None:intergenic 45.0%
GTCGATTGCAAAAACAGTCC+CGG - chr3.1:9120010-9120029 MS.gene039597:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.1:9119955-9119974 None:intergenic 45.0%
TCATATCCAGGTCAAGAAGG+AGG - chr3.1:9120952-9120971 MS.gene039597:CDS 45.0%
TGATGATAAGTGGTTCACCG+AGG - chr3.1:9120842-9120861 MS.gene039597:CDS 45.0%
TGGTTTGAGTCTTCGATTGG+GGG + chr3.1:9119764-9119783 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.1:9119938-9119957 MS.gene039597:CDS 45.0%
! AATGGAAAAGCGTGAGGGAA+AGG + chr3.1:9119925-9119944 None:intergenic 45.0%
! ACCTGAGAAAATGATGGTGC+TGG - chr3.1:9121005-9121024 MS.gene039597:CDS 45.0%
! ATGGAAAAGCGTGAGGGAAA+GGG + chr3.1:9119924-9119943 None:intergenic 45.0%
! TCCAGCACCATCATTTTCTC+AGG + chr3.1:9121009-9121028 None:intergenic 45.0%
!! TGTAGTGAGTGCTGAAACGA+CGG - chr3.1:9120663-9120682 MS.gene039597:CDS 45.0%
AATGGGTGGGACATCAATGG+GGG + chr3.1:9120086-9120105 None:intergenic 50.0%
ATCCAGGTCAAGAAGGAGGA+GGG - chr3.1:9120956-9120975 MS.gene039597:CDS 50.0%
CAATGGGTGGGACATCAATG+GGG + chr3.1:9120087-9120106 None:intergenic 50.0%
GACAATGGGTGGGACATCAA+TGG + chr3.1:9120089-9120108 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.1:9119943-9119962 None:intergenic 50.0%
GAGTCTTCGATTGGGGGATA+TGG + chr3.1:9119758-9119777 None:intergenic 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.1:9119953-9119972 None:intergenic 50.0%
TATCCAGGTCAAGAAGGAGG+AGG - chr3.1:9120955-9120974 MS.gene039597:CDS 50.0%
TGCTTAGGTCGGTTGAAGAG+TGG - chr3.1:9120767-9120786 MS.gene039597:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.1:9120704-9120723 MS.gene039597:CDS 50.0%
! CATGCACGTCGACTACAACA+GGG - chr3.1:9120553-9120572 MS.gene039597:CDS 50.0%
! TCATGCACGTCGACTACAAC+AGG - chr3.1:9120552-9120571 MS.gene039597:CDS 50.0%
!! GTGAGTGCTGAAACGACGGT+AGG - chr3.1:9120667-9120686 MS.gene039597:CDS 55.0%
CTCCCTCCTCCTTCTTGACC+TGG + chr3.1:9120961-9120980 None:intergenic 60.0%
GTGGGACATCAATGGGGGTG+AGG + chr3.1:9120081-9120100 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 9119710 9121080 9119710 ID=MS.gene039597
chr3.1 mRNA 9119710 9121080 9119710 ID=MS.gene039597.t1;Parent=MS.gene039597
chr3.1 exon 9119710 9121080 9119710 ID=MS.gene039597.t1.exon1;Parent=MS.gene039597.t1
chr3.1 CDS 9119710 9121080 9119710 ID=cds.MS.gene039597.t1;Parent=MS.gene039597.t1
Gene Sequence

>MS.gene039597

ATGAGCATCCCTTCCCTAAACATCGTTTCTGCATGTTTCATTAAACCATATCCCCCAATCGAAGACTCAAACCAAATATGTCATTTAACACATTGGGATATTGCCATGTTATCTACACATTATAACCAAAAGGGACTTCTCTTCAAAAAACCAACATCATTACTAAACCAACAACATTTCATAGAGAACCTGGTGGACAAACTGAAACACTCCCTTTCCCTCACGCTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTATACTGTTTTCGTCGATTGCAAAAACAGTCCCGGAGCTAAATTTATTCATGCAACTTTAGATATCACCATAAATGATATCCTCACCCCCATTGATGTCCCACCCATTGTCCAATCGTTCTTTGATCATGACAAAGCAGTCAACCATGATGGTCACACCATGTCTTTGTTATCTATTCAAGTCACAGAATTAGTAGATGGTGTTTTCATTGGTTGTTCCATGAATCATTGTCTCTGTGATGGAACATCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATATTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATTATCAACCTTCCATTTAAACATCACCACGAGTTTGTTCACACATATGGAATACCCATTTTAAAAGAGAGAATCTTCCATTTTTCAGCGGAATCTATAGCGAAACTTAAAGCAAAGGCAAATGCAGAGTCTAATACAAACAAAATTTCTTCGTTTCAATCTTTATCAGCATTAGTTTGGAGATCTATAACTCATGCACGTCGACTACAACAGGGTCAGAGAACAACTTGCAAAATGGCTACAAACAACCGAACAAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCAATTTATGTAGTGAGTGCTGAAACGACGGTAGGAGAGTTGCTAGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATGTGGCTATTGCAAATCATGATAATAAAGTGGTGCTTAGGTCGGTTGAAGAGTGGTTTCGTTCTCCTTTTTTGTATCGATTAGATTTGTTTTTTGATCCTTATACTGTGATGATAAGTGGTTCACCGAGGTTTAATACGTATGGAAATGAATTTGGAATGGGAAAAGCTTTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAATTTCATATCCAGGTCAAGAAGGAGGAGGGAGCATAGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGGTGCTGGAGACAGATGAAGAGTTCATGAACTCAGTTTCTGTGTTTAATCCTTTGTTCTAA
Protein sequence

>MS.gene039597.t1

MSIPSLNIVSACFIKPYPPIEDSNQICHLTHWDIAMLSTHYNQKGLLFKKPTSLLNQQHFIENLVDKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILTPIDVPPIVQSFFDHDKAVNHDGHTMSLLSIQVTELVDGVFIGCSMNHCLCDGTSYWNFFNTFSEIFQNDIPVYGPISHQPIHNRWFPEGYGPIINLPFKHHHEFVHTYGIPILKERIFHFSAESIAKLKAKANAESNTNKISSFQSLSALVWRSITHARRLQQGQRTTCKMATNNRTRMEPPLPKEYFGNSIYVVSAETTVGELLENDLGWAAWKIHVAIANHDNKVVLRSVEEWFRSPFLYRLDLFFDPYTVMISGSPRFNTYGNEFGMGKALAVRSGYANKFDGKVISYPGQEGGGSIDLEVCLSPEKMMVLETDEEFMNSVSVFNPLF