AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38365

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38365.t1 MTR_3g102020 94.311 457 26 0 1 457 1 457 0.0 899
MS.gene38365.t1 MTR_3g101960 88.341 446 51 1 1 446 1 445 0.0 834
MS.gene38365.t1 MTR_3g102010 84.599 461 62 3 1 457 2 457 0.0 807
MS.gene38365.t1 MTR_3g101970 83.628 452 60 1 1 452 1 438 0.0 796
MS.gene38365.t1 MTR_3g101900 68.433 453 137 3 1 452 2 449 0.0 634
MS.gene38365.t1 MTR_3g101930 66.300 454 148 3 1 450 2 454 0.0 623
MS.gene38365.t1 MTR_3g102030 79.245 318 40 2 87 404 29 320 0.0 520
MS.gene38365.t1 MTR_3g070170 39.837 492 217 14 2 447 8 466 1.20e-104 319
MS.gene38365.t1 MTR_4g015180 39.604 505 233 12 1 451 1 487 7.97e-100 308
MS.gene38365.t1 MTR_5g081960 39.044 502 231 12 1 447 1 482 7.19e-99 305
MS.gene38365.t1 MTR_8g041770 39.408 439 247 10 16 451 19 441 1.79e-94 292
MS.gene38365.t1 MTR_4g094842 38.428 458 253 11 4 447 2 444 5.84e-90 281
MS.gene38365.t1 MTR_4g022800 38.182 440 242 11 25 451 20 442 1.58e-88 277
MS.gene38365.t1 MTR_4g022720 39.492 433 241 9 24 451 19 435 7.07e-88 275
MS.gene38365.t1 MTR_4g022825 38.497 439 242 10 24 451 19 440 7.43e-88 275
MS.gene38365.t1 MTR_4g094835 38.372 430 243 8 25 447 28 442 5.57e-87 273
MS.gene38365.t1 MTR_0190s0020 38.084 428 248 8 28 451 28 442 1.58e-86 272
MS.gene38365.t1 MTR_3g088420 38.710 434 241 10 24 451 19 433 4.12e-86 270
MS.gene38365.t1 MTR_4g094838 38.228 429 229 8 28 447 27 428 7.78e-86 270
MS.gene38365.t1 MTR_4g022670 38.269 439 244 10 24 451 19 441 1.88e-84 266
MS.gene38365.t1 MTR_7g026860 37.130 439 246 11 24 451 19 438 2.01e-81 258
MS.gene38365.t1 MTR_4g094828 38.063 444 240 14 26 452 26 451 7.61e-80 254
MS.gene38365.t1 MTR_3g026310 37.472 443 239 12 29 451 24 448 1.43e-79 254
MS.gene38365.t1 MTR_4g022290 36.905 420 232 11 24 430 25 424 7.57e-78 249
MS.gene38365.t1 MTR_8g079710 38.771 423 223 17 25 432 25 426 1.62e-74 241
MS.gene38365.t1 MTR_4g094832 41.834 349 188 8 106 447 86 426 1.02e-71 233
MS.gene38365.t1 MTR_4g022760 35.917 387 212 11 24 387 19 392 2.15e-65 216
MS.gene38365.t1 MTR_6g045553 31.875 480 275 12 5 450 13 474 9.83e-60 203
MS.gene38365.t1 MTR_6g016815 30.858 431 213 12 24 451 33 381 9.08e-47 166
MS.gene38365.t1 MTR_8g040780 36.607 224 137 4 235 454 14 236 2.77e-39 142
MS.gene38365.t1 MTR_8g075610 27.157 394 240 14 69 441 47 414 2.52e-32 127
MS.gene38365.t1 MTR_2g064495 26.753 385 256 13 69 441 56 426 1.34e-31 125
MS.gene38365.t1 MTR_8g096530 31.013 316 178 11 63 359 53 347 5.03e-31 124
MS.gene38365.t1 MTR_2g105330 26.494 385 257 13 69 441 56 426 5.92e-31 124
MS.gene38365.t1 MTR_4g007540 24.619 459 289 17 6 441 3 427 6.82e-31 124
MS.gene38365.t1 MTR_3g049150 27.233 459 252 19 22 441 25 440 1.27e-29 120
MS.gene38365.t1 MTR_7g026850 41.667 156 79 4 43 197 21 165 1.99e-29 114
MS.gene38365.t1 MTR_8g040760 34.685 222 133 5 4 224 2 212 1.11e-28 112
MS.gene38365.t1 MTR_3g098980 29.703 303 186 9 69 359 66 353 1.49e-28 117
MS.gene38365.t1 MTR_4g127650 26.464 461 252 19 19 437 30 445 4.78e-28 116
MS.gene38365.t1 MTR_7g026770 37.288 177 90 3 156 332 132 287 1.48e-26 109
MS.gene38365.t1 MTR_3g026950 24.255 470 273 18 17 446 20 446 1.67e-26 111
MS.gene38365.t1 MTR_4g097090 26.577 444 263 16 29 437 30 445 6.85e-26 110
MS.gene38365.t1 MTR_4g094825 38.000 150 82 2 302 451 253 391 4.24e-24 103
MS.gene38365.t1 MTR_4g094825 30.453 243 138 7 29 255 92 319 4.10e-22 98.2
MS.gene38365.t1 MTR_3g112180 26.812 414 242 16 14 400 11 390 1.77e-23 102
MS.gene38365.t1 MTR_7g027175 29.841 315 195 10 57 359 56 356 2.68e-23 102
MS.gene38365.t1 MTR_6g053810 31.818 286 177 12 70 345 72 349 4.76e-23 101
MS.gene38365.t1 MTR_6g034495 43.220 118 63 3 80 194 45 161 4.86e-23 95.9
MS.gene38365.t1 MTR_5g076700 25.117 426 277 17 33 437 27 431 1.07e-22 100
MS.gene38365.t1 MTR_7g114470 25.532 423 280 16 33 437 28 433 5.40e-22 98.6
MS.gene38365.t1 MTR_3g071320 26.400 375 237 13 3 359 2 355 3.62e-21 95.9
MS.gene38365.t1 MTR_7g114550 26.018 442 278 20 33 449 27 444 7.82e-21 95.1
MS.gene38365.t1 MTR_1g081660 25.185 405 265 14 66 454 70 452 4.69e-20 92.8
MS.gene38365.t1 MTR_1g040195 23.709 426 282 16 48 451 16 420 7.38e-20 92.0
MS.gene38365.t1 MTR_1g040175 26.975 367 231 17 66 420 61 402 7.90e-20 92.0
MS.gene38365.t1 MTR_3g465980 26.712 292 196 8 53 335 52 334 3.00e-19 90.5
MS.gene38365.t1 MTR_4g055170 26.690 281 179 8 69 335 69 336 5.10e-19 89.7
MS.gene38365.t1 MTR_7g086000 26.768 396 238 19 72 432 63 441 1.46e-18 88.2
MS.gene38365.t1 MTR_7g114560 25.285 439 285 19 33 449 27 444 3.36e-18 87.0
MS.gene38365.t1 MTR_3g465930 27.899 276 177 8 69 332 69 334 1.38e-17 85.5
MS.gene38365.t1 MTR_3g465990 26.621 293 195 9 53 335 52 334 2.03e-17 84.7
MS.gene38365.t1 MTR_7g114960 25.815 399 265 11 66 447 60 444 2.94e-17 84.3
MS.gene38365.t1 MTR_3g465950 28.302 265 169 7 70 322 69 324 3.68e-17 84.0
MS.gene38365.t1 MTR_2g015430 25.000 296 204 9 72 359 61 346 9.71e-17 82.4
MS.gene38365.t1 MTR_6g034485 35.043 117 69 2 206 318 2 115 1.38e-16 76.3
MS.gene38365.t1 MTR_6g015850 25.420 417 249 15 61 442 62 451 1.43e-16 82.0
MS.gene38365.t1 MTR_1g040215 27.835 291 190 11 66 348 61 339 1.79e-16 82.0
MS.gene38365.t1 MTR_7g014160 24.788 472 285 18 18 442 19 467 4.06e-16 80.9
MS.gene38365.t1 MTR_3g465910 28.674 279 167 9 70 332 69 331 4.53e-16 80.9
MS.gene38365.t1 MTR_5g026320 24.586 423 262 17 48 446 59 448 4.89e-16 80.5
MS.gene38365.t1 MTR_7g084940 24.038 312 206 10 65 361 65 360 1.01e-15 79.3
MS.gene38365.t1 MTR_4g013050 23.691 401 232 16 67 441 51 403 1.37e-15 79.0
MS.gene38365.t1 MTR_6g015830 24.450 409 266 14 61 442 62 454 2.24e-15 78.6
MS.gene38365.t1 MTR_7g026830 50.000 68 33 1 112 178 28 95 2.42e-14 68.9
MS.gene38365.t1 MTR_7g056087 23.670 376 248 14 6 362 4 359 2.78e-14 75.1
MS.gene38365.t1 MTR_7g014200 23.965 459 306 19 15 442 16 462 7.81e-14 73.6
MS.gene38365.t1 MTR_3g049140 25.980 204 133 5 243 432 27 226 8.46e-14 71.6
MS.gene38365.t1 MTR_7g014620 24.374 439 280 21 29 442 15 426 1.24e-13 72.8
MS.gene38365.t1 MTR_1g040315 26.316 304 198 11 33 326 26 313 4.09e-13 71.2
MS.gene38365.t1 MTR_7g085990 23.284 408 264 14 66 437 52 446 4.52e-13 71.2
MS.gene38365.t1 MTR_6g015320 23.529 425 273 16 42 437 42 443 4.65e-13 71.2
MS.gene38365.t1 MTR_3g450710 23.473 311 175 11 63 337 59 342 5.27e-13 71.2
MS.gene38365.t1 MTR_7g013850 23.278 421 258 15 32 403 32 436 2.10e-12 69.3
MS.gene38365.t1 MTR_6g015315 24.574 411 266 19 54 437 48 441 2.62e-11 65.9
MS.gene38365.t1 MTR_8g079970 24.496 347 206 14 6 331 3 314 3.92e-11 65.1
MS.gene38365.t1 MTR_5g090830 23.779 307 187 15 64 343 61 347 6.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38365.t1 AT5G42830 56.659 443 183 2 13 452 14 450 0.0 525
MS.gene38365.t1 AT5G07850 54.102 451 205 2 2 451 4 453 1.24e-180 513
MS.gene38365.t1 AT5G07860 53.778 450 198 5 4 450 6 448 3.28e-168 481
MS.gene38365.t1 AT5G07870 50.433 462 213 3 2 451 4 461 2.52e-164 471
MS.gene38365.t1 AT2G39980 40.254 472 213 9 29 451 29 480 1.33e-104 320
MS.gene38365.t1 AT5G67150 39.216 459 257 11 3 452 2 447 1.99e-100 308
MS.gene38365.t1 AT5G01210 38.900 491 238 12 1 451 1 469 3.49e-93 290
MS.gene38365.t1 AT3G50270 35.129 464 274 12 1 452 1 449 4.93e-82 260
MS.gene38365.t1 AT3G50280 34.735 452 273 11 4 447 2 439 1.11e-76 246
MS.gene38365.t1 AT3G50300 32.751 458 280 9 6 450 4 446 4.22e-74 240
MS.gene38365.t1 AT5G38130 35.068 442 253 14 6 434 24 444 7.41e-73 237
MS.gene38365.t1 AT5G67160 34.965 429 252 11 28 452 28 433 2.10e-70 230
MS.gene38365.t1 AT5G23940 33.913 460 276 11 6 449 3 450 4.03e-66 220
MS.gene38365.t1 AT3G50280 37.459 307 181 6 147 447 8 309 1.35e-54 185
MS.gene38365.t1 AT3G50290 37.452 259 149 6 148 397 15 269 2.99e-43 154
MS.gene38365.t1 AT5G48930 25.821 457 286 16 6 441 3 427 1.26e-33 131
MS.gene38365.t1 AT2G19070 29.025 441 256 18 29 437 25 440 2.96e-33 130
MS.gene38365.t1 AT5G41040 29.526 359 206 15 63 400 63 395 3.52e-32 127
MS.gene38365.t1 AT5G41040 29.526 359 206 15 63 400 79 411 4.94e-32 127
MS.gene38365.t1 AT3G48720 26.790 433 268 17 1 421 1 396 3.85e-24 104
MS.gene38365.t1 AT2G40230 27.632 380 206 14 64 410 54 397 8.48e-23 100
MS.gene38365.t1 AT5G63560 29.167 312 185 12 63 359 55 345 2.63e-22 99.4
MS.gene38365.t1 AT1G65450 26.935 323 201 12 57 359 60 367 2.48e-21 96.7
MS.gene38365.t1 AT1G27620 26.984 315 182 10 69 359 58 348 2.73e-20 93.2
MS.gene38365.t1 AT5G57840 26.190 420 251 16 46 437 39 427 4.32e-20 92.8
MS.gene38365.t1 AT1G28680 26.014 296 200 11 72 359 65 349 4.76e-19 89.4
MS.gene38365.t1 AT1G28680 26.014 296 200 11 72 359 65 349 5.97e-19 89.4
MS.gene38365.t1 AT5G16410 22.747 466 280 17 24 456 56 474 1.59e-15 79.0
MS.gene38365.t1 AT1G03390 25.310 403 252 18 54 432 64 441 1.98e-15 78.6
MS.gene38365.t1 AT1G78990 24.430 307 196 11 48 343 52 333 2.03e-15 78.6
MS.gene38365.t1 AT2G25150 27.667 300 185 12 62 345 60 343 2.03e-15 78.6
MS.gene38365.t1 AT1G24420 26.552 467 292 19 6 457 5 435 6.21e-15 77.0
MS.gene38365.t1 AT1G24420 26.552 467 292 19 6 457 5 435 6.21e-15 77.0
MS.gene38365.t1 AT5G07080 25.090 279 173 11 70 330 71 331 7.05e-14 73.9
MS.gene38365.t1 AT1G65450 25.837 209 133 6 167 359 1 203 1.49e-13 71.2
MS.gene38365.t1 AT2G23510 26.667 255 171 7 69 317 69 313 8.55e-12 67.4
MS.gene38365.t1 AT5G23970 23.965 459 288 19 6 447 3 417 1.01e-11 67.0
MS.gene38365.t1 AT1G03940 23.005 426 267 21 57 442 54 458 1.01e-11 67.4
MS.gene38365.t1 AT5G39090 22.560 461 307 16 5 440 4 439 1.28e-11 67.0
MS.gene38365.t1 AT3G29590 22.997 387 256 14 76 437 68 437 8.32e-11 64.3

Find 104 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTTCCTAAGGAATATTT+TGG 0.126132 3.2:-84862712 MS.gene38365:CDS
CATTCAAGTTACTGAATTAT+TGG 0.137906 3.2:-84863169 MS.gene38365:CDS
AATATGTATGGGAATGAATT+TGG 0.211414 3.2:-84862472 MS.gene38365:CDS
AATGTGATAAATGGCATATT+TGG 0.269841 3.2:+84863562 None:intergenic
AATATTCTCTCTCTCAAAAT+AGG 0.288986 3.2:+84862925 None:intergenic
AGGAAGAGCTGGTTCCATTC+TGG 0.290483 3.2:+84862726 None:intergenic
TAATATCCCAATGTGATAAA+TGG 0.297305 3.2:+84863553 None:intergenic
AATCTTTATCAGCACTAGTT+TGG 0.302845 3.2:-84862822 MS.gene38365:CDS
TCAACGGACAACTTGCAAAT+TGG 0.320331 3.2:-84862764 MS.gene38365:CDS
CTACCCATCATCACAGTATA+AGG 0.343091 3.2:+84862508 None:intergenic
CATTCTGGTTCGGTTGTTTA+CGG 0.353477 3.2:+84862741 None:intergenic
GGAAACCATCTATTGTGAAT+AGG 0.358486 3.2:+84863018 None:intergenic
ATAAACCTATTCACAATAGA+TGG 0.359431 3.2:-84863023 MS.gene38365:CDS
CTTCCTCCTCCTTCTTTACC+TGG 0.359731 3.2:+84862382 None:intergenic
TCAAAGAATGATTGAACAAT+AGG 0.361615 3.2:+84863240 None:intergenic
ATGATTGGACCATAACCTTC+TGG 0.364910 3.2:+84862997 None:intergenic
AGATGAAGAGTTCATGAATT+CGG 0.367038 3.2:-84862308 MS.gene38365:CDS
ATCTGAGAAAATGATGTTGC+TGG 0.375240 3.2:-84862335 MS.gene38365:CDS
TCACCAAGGTTCAATATGTA+TGG 0.377799 3.2:-84862484 MS.gene38365:CDS
TTCACCACTCACCGCATAGA+TGG 0.382856 3.2:+84862684 None:intergenic
TGAATAGGTTTATGTGAAAT+CGG 0.391414 3.2:+84863033 None:intergenic
TTGGTGATGGAACTGGTTAT+TGG 0.394500 3.2:-84863110 MS.gene38365:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3.2:+84863125 None:intergenic
GTCGATTGCGAAAACAGTCC+TGG 0.405346 3.2:-84863330 MS.gene38365:CDS
GGATATGCTAACAAATTTGA+TGG 0.406437 3.2:-84862421 MS.gene38365:CDS
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.2:-84863006 MS.gene38365:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.425196 3.2:-84863402 MS.gene38365:CDS
ACAATAGGTGGCACATCAAC+AGG 0.427451 3.2:+84863255 None:intergenic
AATATGCCATTTATCACATT+GGG 0.437912 3.2:-84863559 MS.gene38365:CDS
AGTGTTTCAGTTTCTCTACT+AGG 0.438345 3.2:+84863442 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3.2:+84863463 None:intergenic
ATTTGGTTCAAGTCTTCGAT+TGG 0.440400 3.2:+84863579 None:intergenic
TGATCCTTATACTGTGATGA+TGG 0.442813 3.2:-84862512 MS.gene38365:CDS
GAGCTGGTTCCATTCTGGTT+CGG 0.444220 3.2:+84862731 None:intergenic
TTTGGTTCAAGTCTTCGATT+GGG 0.445265 3.2:+84863580 None:intergenic
CATTGTATTGGTGATGGAAC+TGG 0.455215 3.2:-84863117 MS.gene38365:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.2:+84863359 None:intergenic
AATGGAAAAGTGTGAGGGAA+AGG 0.463707 3.2:+84863418 None:intergenic
TTGAAAGGAAGGTTGATGAT+TGG 0.467597 3.2:+84862982 None:intergenic
TGATAAAGTGGTGCGAAATT+CGG 0.474906 3.2:-84862584 MS.gene38365:CDS
AGAGTTCATGAATTCGGTTT+CGG 0.476951 3.2:-84862302 MS.gene38365:CDS
GAATTCCCAAAATATTCCTT+AGG 0.479625 3.2:+84862706 None:intergenic
CATCATAATTTGCAATAGCC+AGG 0.479956 3.2:+84862605 None:intergenic
CAATAATTCAGTAACTTGAA+TGG 0.486762 3.2:+84863170 None:intergenic
AACAACGGTAGGGGAATTGC+TGG 0.488208 3.2:-84862662 MS.gene38365:CDS
CGTCGTGATGTTTGAAAGGA+AGG 0.488568 3.2:+84862971 None:intergenic
ATGGAAAAGTGTGAGGGAAA+GGG 0.490102 3.2:+84863419 None:intergenic
AAATATGCCATTTATCACAT+TGG 0.490158 3.2:-84863560 MS.gene38365:CDS
GGTTTATGTGAAATCGGAAC+AGG 0.490426 3.2:+84863039 None:intergenic
ATCTGCATACTATATCCAAA+AGG 0.491658 3.2:-84863523 MS.gene38365:CDS
TGTTCCATGAATCATTGTAT+TGG 0.493564 3.2:-84863129 MS.gene38365:CDS
AAATATTCCTTAGGAAGAGC+TGG 0.504661 3.2:+84862715 None:intergenic
AGGTGAGAGAATATCATTGA+TGG 0.504727 3.2:+84863275 None:intergenic
GAATTGCTGGAAAATGATCT+AGG 0.510030 3.2:-84862649 MS.gene38365:CDS
CAAGTTACTGAATTATTGGA+TGG 0.511663 3.2:-84863165 MS.gene38365:CDS
TGCTGGAAAATGATCTAGGA+TGG 0.517189 3.2:-84862645 MS.gene38365:CDS
TTCCCATACATATTGAACCT+TGG 0.519386 3.2:+84862481 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.2:-84862466 MS.gene38365:CDS
AGATCCATTCGATACACAAA+AGG 0.526309 3.2:+84862541 None:intergenic
ATTTGGAATGGGAAAAGCAT+TGG 0.528592 3.2:-84862455 MS.gene38365:CDS
TGGTTTAGTGATGATGATGT+TGG 0.530066 3.2:+84863483 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.2:-84863123 MS.gene38365:CDS
GCATGAATAAATTTAGCTCC+AGG 0.532002 3.2:+84863312 None:intergenic
ACATCATATCCAGGTAAAGA+AGG 0.534700 3.2:-84862391 MS.gene38365:CDS
GGAAAAGTAACATCATATCC+AGG 0.542909 3.2:-84862400 MS.gene38365:CDS
AACTCGTCGTGATGTTTGAA+AGG 0.543380 3.2:+84862967 None:intergenic
CACAGAGCTGTCAACCATGA+TGG 0.543528 3.2:-84863213 MS.gene38365:CDS
CACAATAGATGGTTTCCAGA+AGG 0.548245 3.2:-84863012 MS.gene38365:CDS
CGTAAACAACCGAACCAGAA+TGG 0.549236 3.2:-84862740 MS.gene38365:CDS
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.2:+84863389 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.2:+84863400 None:intergenic
TGCAAATTATGATGATAAAG+TGG 0.556314 3.2:-84862596 MS.gene38365:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.557831 3.2:-84862636 MS.gene38365:CDS
CTCATCAAAGACTAAAACAA+AGG 0.560969 3.2:+84862271 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.2:-84862483 MS.gene38365:CDS
TCTGCATACTATATCCAAAA+GGG 0.562084 3.2:-84863522 MS.gene38365:CDS
AATGGAACCAGCTCTTCCTA+AGG 0.569685 3.2:-84862722 MS.gene38365:CDS
GTCATTTACAACATGGTCAA+CGG 0.571152 3.2:-84862780 MS.gene38365:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.2:-84862467 MS.gene38365:CDS
GAGTGGTGAAACAACGGTAG+GGG 0.575446 3.2:-84862671 MS.gene38365:CDS
TGATAAAACATTCAGAAACA+AGG 0.589803 3.2:+84863613 None:intergenic
GATCCTTATACTGTGATGAT+GGG 0.590324 3.2:-84862511 MS.gene38365:CDS
GGCAGCATGGAAGATTCACC+TGG 0.593449 3.2:-84862623 MS.gene38365:CDS
AAGAATGATTGAACAATAGG+TGG 0.594549 3.2:+84863243 None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG 0.597903 3.2:-84862442 MS.gene38365:CDS
TATCCAGGTAAAGAAGGAGG+AGG 0.601869 3.2:-84862385 MS.gene38365:CDS
TGAGTGGTGAAACAACGGTA+GGG 0.603070 3.2:-84862672 MS.gene38365:CDS
TTGGTTCAAGTCTTCGATTG+GGG 0.606286 3.2:+84863581 None:intergenic
TGCGAAATTCGGTTGAAGAG+TGG 0.613912 3.2:-84862573 MS.gene38365:CDS
GATAGAAATGGAAAAGTGTG+AGG 0.622911 3.2:+84863412 None:intergenic
GCTGGAAAATGATCTAGGAT+GGG 0.626677 3.2:-84862644 MS.gene38365:CDS
CGTGCACGTCATTTACAACA+TGG 0.627245 3.2:-84862787 MS.gene38365:CDS
TGCGGTGAGTGGTGAAACAA+CGG 0.630833 3.2:-84862677 MS.gene38365:CDS
GTGAGTGGTGAAACAACGGT+AGG 0.635845 3.2:-84862673 MS.gene38365:CDS
AAGACATGGTGTGTCCATCA+TGG 0.638582 3.2:+84863199 None:intergenic
TCATATCCAGGTAAAGAAGG+AGG 0.645386 3.2:-84862388 MS.gene38365:CDS
TGGTTCAAGTCTTCGATTGG+GGG 0.657471 3.2:+84863582 None:intergenic
TTGAATGGACAACAAAGACA+TGG 0.674790 3.2:+84863185 None:intergenic
AATTCCATCTATGCGGTGAG+TGG 0.681160 3.2:-84862688 MS.gene38365:CDS
ATAGAAATGGAAAAGTGTGA+GGG 0.681846 3.2:+84863413 None:intergenic
CATCATGGTTGACAGCTCTG+TGG 0.688486 3.2:+84863214 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.2:+84863388 None:intergenic
TGATGATGGGTAGCTCACCA+AGG 0.697945 3.2:-84862498 MS.gene38365:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.2:+84863390 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGTATGGGAATGAATT+TGG - chr3.2:84863443-84863462 MS.gene38365:CDS 25.0%
! AATATTCTCTCTCTCAAAAT+AGG + chr3.2:84862993-84863012 None:intergenic 25.0%
! AATGTGATAAATGGCATATT+TGG + chr3.2:84862356-84862375 None:intergenic 25.0%
! ATAAACCTATTCACAATAGA+TGG - chr3.2:84862892-84862911 MS.gene38365:CDS 25.0%
! CAATAATTCAGTAACTTGAA+TGG + chr3.2:84862748-84862767 None:intergenic 25.0%
! TAATATCCCAATGTGATAAA+TGG + chr3.2:84862365-84862384 None:intergenic 25.0%
! TCAAAGAATGATTGAACAAT+AGG + chr3.2:84862678-84862697 None:intergenic 25.0%
! TGAATAGGTTTATGTGAAAT+CGG + chr3.2:84862885-84862904 None:intergenic 25.0%
! TGATAAAACATTCAGAAACA+AGG + chr3.2:84862305-84862324 None:intergenic 25.0%
! TGCAAATTATGATGATAAAG+TGG - chr3.2:84863319-84863338 MS.gene38365:CDS 25.0%
!! AAATATGCCATTTATCACAT+TGG - chr3.2:84862355-84862374 MS.gene38365:CDS 25.0%
!! AATATGCCATTTATCACATT+GGG - chr3.2:84862356-84862375 MS.gene38365:CDS 25.0%
!! CATTCAAGTTACTGAATTAT+TGG - chr3.2:84862746-84862765 MS.gene38365:CDS 25.0%
!!! TTATTGGATGGTGTTTTTAT+AGG - chr3.2:84862762-84862781 MS.gene38365:CDS 25.0%
AAGAATGATTGAACAATAGG+TGG + chr3.2:84862675-84862694 None:intergenic 30.0%
AATCTTTATCAGCACTAGTT+TGG - chr3.2:84863093-84863112 MS.gene38365:CDS 30.0%
ACAAAAACAGTGTAAGAATG+AGG + chr3.2:84862567-84862586 None:intergenic 30.0%
AGATGAAGAGTTCATGAATT+CGG - chr3.2:84863607-84863626 MS.gene38365:CDS 30.0%
ATCACCAATACAATGATTCA+TGG + chr3.2:84862793-84862812 None:intergenic 30.0%
ATCTGCATACTATATCCAAA+AGG - chr3.2:84862392-84862411 MS.gene38365:CDS 30.0%
CAAGTTACTGAATTATTGGA+TGG - chr3.2:84862750-84862769 MS.gene38365:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.2:84863212-84863231 None:intergenic 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.2:84863494-84863513 MS.gene38365:CDS 30.0%
TCTGCATACTATATCCAAAA+GGG - chr3.2:84862393-84862412 MS.gene38365:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.2:84862786-84862805 MS.gene38365:CDS 30.0%
! ATAGAAATGGAAAAGTGTGA+GGG + chr3.2:84862505-84862524 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.2:84862792-84862811 MS.gene38365:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.2:84863204-84863223 MS.gene38365:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.2:84863449-84863468 MS.gene38365:CDS 30.0%
AAATATTCCTTAGGAAGAGC+TGG + chr3.2:84863203-84863222 None:intergenic 35.0%
ACATCATATCCAGGTAAAGA+AGG - chr3.2:84863524-84863543 MS.gene38365:CDS 35.0%
AGAGTTCATGAATTCGGTTT+CGG - chr3.2:84863613-84863632 MS.gene38365:CDS 35.0%
AGATCCATTCGATACACAAA+AGG + chr3.2:84863377-84863396 None:intergenic 35.0%
AGGTGAGAGAATATCATTGA+TGG + chr3.2:84862643-84862662 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.2:84862559-84862578 None:intergenic 35.0%
AGTGTTTCAGTTTCTCTACT+AGG + chr3.2:84862476-84862495 None:intergenic 35.0%
ATCTGAGAAAATGATGTTGC+TGG - chr3.2:84863580-84863599 MS.gene38365:CDS 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.2:84863432-84863451 MS.gene38365:CDS 35.0%
GAATTGCTGGAAAATGATCT+AGG - chr3.2:84863266-84863285 MS.gene38365:CDS 35.0%
GATCCTTATACTGTGATGAT+GGG - chr3.2:84863404-84863423 MS.gene38365:CDS 35.0%
GGAAAAGTAACATCATATCC+AGG - chr3.2:84863515-84863534 MS.gene38365:CDS 35.0%
GGAAACCATCTATTGTGAAT+AGG + chr3.2:84862900-84862919 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.2:84862455-84862474 None:intergenic 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.2:84863448-84863467 MS.gene38365:CDS 35.0%
GTCATTTACAACATGGTCAA+CGG - chr3.2:84863135-84863154 MS.gene38365:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.2:84863431-84863450 MS.gene38365:CDS 35.0%
TGATAAAGTGGTGCGAAATT+CGG - chr3.2:84863331-84863350 MS.gene38365:CDS 35.0%
TGATCCTTATACTGTGATGA+TGG - chr3.2:84863403-84863422 MS.gene38365:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.2:84863437-84863456 None:intergenic 35.0%
TTGAATGGACAACAAAGACA+TGG + chr3.2:84862733-84862752 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCAT+TGG - chr3.2:84863460-84863479 MS.gene38365:CDS 35.0%
! ATTTGGTTCAAGTCTTCGAT+TGG + chr3.2:84862339-84862358 None:intergenic 35.0%
! CATCATAATTTGCAATAGCC+AGG + chr3.2:84863313-84863332 None:intergenic 35.0%
! GATAGAAATGGAAAAGTGTG+AGG + chr3.2:84862506-84862525 None:intergenic 35.0%
! GCATGAATAAATTTAGCTCC+AGG + chr3.2:84862606-84862625 None:intergenic 35.0%
! GCTCTTCCTAAGGAATATTT+TGG - chr3.2:84863203-84863222 MS.gene38365:CDS 35.0%
! TGGTTTAGTGATGATGATGT+TGG + chr3.2:84862435-84862454 None:intergenic 35.0%
! TTCTCCTTTTGTGTATCGAA+TGG - chr3.2:84863370-84863389 MS.gene38365:CDS 35.0%
! TTTGGTTCAAGTCTTCGATT+GGG + chr3.2:84862338-84862357 None:intergenic 35.0%
!! TTGAAAGGAAGGTTGATGAT+TGG + chr3.2:84862936-84862955 None:intergenic 35.0%
!! TTTTGAAGAGAAGACCCTTT+TGG + chr3.2:84862410-84862429 None:intergenic 35.0%
!! TTTTGGGAATTCCATCTATG+CGG - chr3.2:84863220-84863239 MS.gene38365:CDS 35.0%
AACTCGTCGTGATGTTTGAA+AGG + chr3.2:84862951-84862970 None:intergenic 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.2:84862909-84862928 MS.gene38365:CDS 40.0%
ATGATTGGACCATAACCTTC+TGG + chr3.2:84862921-84862940 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.2:84862903-84862922 MS.gene38365:CDS 40.0%
CTACCCATCATCACAGTATA+AGG + chr3.2:84863410-84863429 None:intergenic 40.0%
GGTTTATGTGAAATCGGAAC+AGG + chr3.2:84862879-84862898 None:intergenic 40.0%
TCAACGGACAACTTGCAAAT+TGG - chr3.2:84863151-84863170 MS.gene38365:CDS 40.0%
TCATATCCAGGTAAAGAAGG+AGG - chr3.2:84863527-84863546 MS.gene38365:CDS 40.0%
TGCTGGAAAATGATCTAGGA+TGG - chr3.2:84863270-84863289 MS.gene38365:CDS 40.0%
TTGGTTCAAGTCTTCGATTG+GGG + chr3.2:84862337-84862356 None:intergenic 40.0%
! AATGGAAAAGTGTGAGGGAA+AGG + chr3.2:84862500-84862519 None:intergenic 40.0%
! ATGGAAAAGTGTGAGGGAAA+GGG + chr3.2:84862499-84862518 None:intergenic 40.0%
! GCTGGAAAATGATCTAGGAT+GGG - chr3.2:84863271-84863290 MS.gene38365:CDS 40.0%
!! AAAGCATTGGCAGTTAGAAG+TGG - chr3.2:84863473-84863492 MS.gene38365:CDS 40.0%
!! CATTCTGGTTCGGTTGTTTA+CGG + chr3.2:84863177-84863196 None:intergenic 40.0%
!! CATTGTATTGGTGATGGAAC+TGG - chr3.2:84862798-84862817 MS.gene38365:CDS 40.0%
!! TTGGTGATGGAACTGGTTAT+TGG - chr3.2:84862805-84862824 MS.gene38365:CDS 40.0%
AAGACATGGTGTGTCCATCA+TGG + chr3.2:84862719-84862738 None:intergenic 45.0%
AATGGAACCAGCTCTTCCTA+AGG - chr3.2:84863193-84863212 MS.gene38365:CDS 45.0%
AATTCCATCTATGCGGTGAG+TGG - chr3.2:84863227-84863246 MS.gene38365:CDS 45.0%
ACAATAGGTGGCACATCAAC+AGG + chr3.2:84862663-84862682 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.2:84862529-84862548 None:intergenic 45.0%
CGTAAACAACCGAACCAGAA+TGG - chr3.2:84863175-84863194 MS.gene38365:CDS 45.0%
CGTCGTGATGTTTGAAAGGA+AGG + chr3.2:84862947-84862966 None:intergenic 45.0%
CGTGCACGTCATTTACAACA+TGG - chr3.2:84863128-84863147 MS.gene38365:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.2:84862530-84862549 None:intergenic 45.0%
TATCCAGGTAAAGAAGGAGG+AGG - chr3.2:84863530-84863549 MS.gene38365:CDS 45.0%
TGAGTGGTGAAACAACGGTA+GGG - chr3.2:84863243-84863262 MS.gene38365:CDS 45.0%
TGCGAAATTCGGTTGAAGAG+TGG - chr3.2:84863342-84863361 MS.gene38365:CDS 45.0%
TGGTTCAAGTCTTCGATTGG+GGG + chr3.2:84862336-84862355 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.2:84862513-84862532 MS.gene38365:CDS 45.0%
AACAACGGTAGGGGAATTGC+TGG - chr3.2:84863253-84863272 MS.gene38365:CDS 50.0%
AAGTCTTCGATTGGGGGACA+TGG + chr3.2:84862330-84862349 None:intergenic 50.0%
CACAGAGCTGTCAACCATGA+TGG - chr3.2:84862702-84862721 MS.gene38365:CDS 50.0%
CATCATGGTTGACAGCTCTG+TGG + chr3.2:84862704-84862723 None:intergenic 50.0%
CTTCCTCCTCCTTCTTTACC+TGG + chr3.2:84863536-84863555 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.2:84862518-84862537 None:intergenic 50.0%
GAGTGGTGAAACAACGGTAG+GGG - chr3.2:84863244-84863263 MS.gene38365:CDS 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.2:84862528-84862547 None:intergenic 50.0%
GTCGATTGCGAAAACAGTCC+TGG - chr3.2:84862585-84862604 MS.gene38365:CDS 50.0%
GTGAGTGGTGAAACAACGGT+AGG - chr3.2:84863242-84863261 MS.gene38365:CDS 50.0%
TGCGGTGAGTGGTGAAACAA+CGG - chr3.2:84863238-84863257 MS.gene38365:CDS 50.0%
TTCACCACTCACCGCATAGA+TGG + chr3.2:84863234-84863253 None:intergenic 50.0%
! AGGAAGAGCTGGTTCCATTC+TGG + chr3.2:84863192-84863211 None:intergenic 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.2:84863279-84863298 MS.gene38365:CDS 50.0%
! GAGCTGGTTCCATTCTGGTT+CGG + chr3.2:84863187-84863206 None:intergenic 50.0%
!! TGATGATGGGTAGCTCACCA+AGG - chr3.2:84863417-84863436 MS.gene38365:CDS 50.0%
GGCAGCATGGAAGATTCACC+TGG - chr3.2:84863292-84863311 MS.gene38365:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 84862282 84863655 84862282 ID=MS.gene38365
chr3.2 mRNA 84862282 84863655 84862282 ID=MS.gene38365.t1;Parent=MS.gene38365
chr3.2 exon 84862282 84863655 84862282 ID=MS.gene38365.t1.exon1;Parent=MS.gene38365.t1
chr3.2 CDS 84862282 84863655 84862282 ID=cds.MS.gene38365.t1;Parent=MS.gene38365.t1
Gene Sequence

>MS.gene38365

ATGAACGCTTCTTCTCTAAACCTTGTTTCTGAATGTTTTATCAAACCATGTCCCCCAATCGAAGACTTGAACCAAATATGCCATTTATCACATTGGGATATTAGCATGTTATCTGCATACTATATCCAAAAGGGTCTTCTCTTCAAAAAACCAACATCATCATCACTAAACCAACAACATTTCATAGAGAACCTAGTAGAGAAACTGAAACACTCCCTTTCCCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTTGTCGATTGCGAAAACAGTCCTGGAGCTAAATTTATTCATGCTACTTTAGATATCACCATCAATGATATTCTCTCACCTGTTGATGTGCCACCTATTGTTCAATCATTCTTTGATCACCACAGAGCTGTCAACCATGATGGACACACCATGTCTTTGTTGTCCATTCAAGTTACTGAATTATTGGATGGTGTTTTTATAGGTTGTTCCATGAATCATTGTATTGGTGATGGAACTGGTTATTGGAATTTCTTTAACTCATTTTCTGAGATATTTCAAAATGATGTTCATGTTCCTGTTCCGATTTCACATAAACCTATTCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATCATCAACCTTCCTTTCAAACATCACGACGAGTTTATTCATAGATATGAAACACCTATTTTGAGAGAGAGAATATTTCATTTTTCAGCAGAGTCTATTGCAAAACTTAAAGCAAAAGCTAACAAAGAGTCTGATACAAACAAAATCTCTTCTTTTCAATCTTTATCAGCACTAGTTTGGAGATCTCTAACGCGTGCACGTCATTTACAACATGGTCAACGGACAACTTGCAAATTGGCCGTAAACAACCGAACCAGAATGGAACCAGCTCTTCCTAAGGAATATTTTGGGAATTCCATCTATGCGGTGAGTGGTGAAACAACGGTAGGGGAATTGCTGGAAAATGATCTAGGATGGGCAGCATGGAAGATTCACCTGGCTATTGCAAATTATGATGATAAAGTGGTGCGAAATTCGGTTGAAGAGTGGTTACGTTCTCCTTTTGTGTATCGAATGGATCTGTTCTTTGATCCTTATACTGTGATGATGGGTAGCTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCAGGTAAAGAAGGAGGAGGAAGCATTGATCTTGAAGTGTGTCTTTCATCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCGGTTTCGGTGTTTAATCCTTTGTTTTAG
Protein sequence

>MS.gene38365.t1

MNASSLNLVSECFIKPCPPIEDLNQICHLSHWDISMLSAYYIQKGLLFKKPTSSSLNQQHFIENLVEKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCENSPGAKFIHATLDITINDILSPVDVPPIVQSFFDHHRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHCIGDGTGYWNFFNSFSEIFQNDVHVPVPISHKPIHNRWFPEGYGPIINLPFKHHDEFIHRYETPILRERIFHFSAESIAKLKAKANKESDTNKISSFQSLSALVWRSLTRARHLQHGQRTTCKLAVNNRTRMEPALPKEYFGNSIYAVSGETTVGELLENDLGWAAWKIHLAIANYDDKVVRNSVEEWLRSPFVYRMDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGKEGGGSIDLEVCLSSEKMMLLETDEEFMNSVSVFNPLF