AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012960.t1 MTR_3g101930 93.612 454 29 0 1 454 2 455 0.0 896
MS.gene012960.t1 MTR_3g101900 72.467 454 116 3 1 453 2 447 0.0 687
MS.gene012960.t1 MTR_3g102020 66.740 454 146 3 1 453 1 450 0.0 629
MS.gene012960.t1 MTR_3g101960 67.621 454 133 5 1 449 1 445 0.0 626
MS.gene012960.t1 MTR_3g101970 65.639 454 137 3 1 453 1 436 0.0 617
MS.gene012960.t1 MTR_3g102010 63.736 455 157 5 1 453 2 450 0.0 594
MS.gene012960.t1 MTR_3g102030 56.522 322 110 4 87 408 29 320 2.22e-129 377
MS.gene012960.t1 MTR_4g094842 40.523 459 245 13 4 450 2 444 2.05e-99 305
MS.gene012960.t1 MTR_4g094835 40.553 434 231 9 25 450 28 442 3.44e-95 294
MS.gene012960.t1 MTR_0190s0020 39.683 441 245 9 18 453 17 441 4.56e-95 294
MS.gene012960.t1 MTR_3g070170 37.166 487 238 12 3 450 9 466 1.15e-94 293
MS.gene012960.t1 MTR_4g022800 39.013 446 239 12 21 453 16 441 5.31e-93 288
MS.gene012960.t1 MTR_8g041770 39.030 433 234 10 29 453 30 440 8.92e-92 285
MS.gene012960.t1 MTR_4g022720 39.726 438 238 10 21 452 16 433 2.84e-90 281
MS.gene012960.t1 MTR_4g094838 37.296 429 234 6 29 450 28 428 9.96e-90 280
MS.gene012960.t1 MTR_4g022670 37.978 445 244 10 21 453 16 440 2.01e-89 279
MS.gene012960.t1 MTR_3g088420 38.532 436 242 10 23 453 18 432 2.64e-89 278
MS.gene012960.t1 MTR_4g015180 37.278 507 243 12 1 453 1 486 5.90e-87 274
MS.gene012960.t1 MTR_7g026860 37.246 443 245 11 21 452 16 436 6.92e-87 273
MS.gene012960.t1 MTR_5g081960 35.178 506 248 12 1 450 1 482 2.21e-85 270
MS.gene012960.t1 MTR_4g022825 37.336 458 253 11 7 453 5 439 1.25e-84 266
MS.gene012960.t1 MTR_4g094832 41.547 349 191 4 107 450 86 426 1.28e-81 259
MS.gene012960.t1 MTR_3g026310 37.220 446 237 11 29 453 24 447 4.36e-81 258
MS.gene012960.t1 MTR_4g094828 37.303 445 241 14 26 453 26 449 9.86e-79 252
MS.gene012960.t1 MTR_4g022290 35.205 463 259 12 7 452 5 443 1.57e-76 246
MS.gene012960.t1 MTR_8g079710 36.384 437 241 12 21 443 21 434 1.18e-71 233
MS.gene012960.t1 MTR_4g022760 34.831 445 214 11 21 453 16 396 2.20e-71 231
MS.gene012960.t1 MTR_6g045553 29.668 482 290 11 3 453 11 474 1.91e-52 183
MS.gene012960.t1 MTR_6g016815 31.235 429 205 13 29 453 38 380 2.14e-49 173
MS.gene012960.t1 MTR_8g040780 36.598 194 120 2 245 435 20 213 2.74e-35 131
MS.gene012960.t1 MTR_8g075610 28.108 370 215 14 69 417 47 386 8.13e-34 132
MS.gene012960.t1 MTR_4g007540 27.957 372 228 13 55 409 44 392 1.95e-33 131
MS.gene012960.t1 MTR_8g096530 32.278 316 176 12 63 362 53 346 2.82e-33 130
MS.gene012960.t1 MTR_2g064495 26.601 406 259 12 55 444 44 426 1.11e-32 129
MS.gene012960.t1 MTR_7g026850 43.949 157 73 5 43 198 21 163 1.35e-32 122
MS.gene012960.t1 MTR_2g105330 26.570 414 249 14 55 444 44 426 2.67e-32 127
MS.gene012960.t1 MTR_3g098980 30.164 305 184 9 69 362 66 352 1.15e-31 126
MS.gene012960.t1 MTR_7g026770 41.209 182 83 5 157 338 132 289 1.43e-29 117
MS.gene012960.t1 MTR_3g026950 25.061 411 243 12 63 433 48 433 6.89e-29 118
MS.gene012960.t1 MTR_8g040760 35.683 227 128 6 4 228 2 212 2.74e-27 108
MS.gene012960.t1 MTR_3g049150 28.803 309 184 9 57 349 46 334 2.86e-27 114
MS.gene012960.t1 MTR_6g034495 44.262 122 63 3 80 198 45 164 2.95e-27 107
MS.gene012960.t1 MTR_4g127650 25.800 469 286 19 8 444 11 449 3.20e-27 114
MS.gene012960.t1 MTR_4g094825 37.241 145 81 1 306 450 253 387 2.26e-25 107
MS.gene012960.t1 MTR_4g094825 37.126 167 96 3 29 194 92 250 9.72e-24 102
MS.gene012960.t1 MTR_7g027175 27.094 406 251 14 56 444 55 432 5.23e-25 107
MS.gene012960.t1 MTR_3g112180 26.934 349 229 9 66 404 58 390 1.86e-23 102
MS.gene012960.t1 MTR_4g097090 25.000 456 268 18 1 417 1 421 4.62e-23 101
MS.gene012960.t1 MTR_7g084940 28.490 351 210 12 67 402 67 391 4.99e-23 101
MS.gene012960.t1 MTR_1g040195 28.969 359 213 16 77 423 48 376 1.44e-22 100
MS.gene012960.t1 MTR_7g086000 29.904 311 181 13 72 362 63 356 1.09e-21 97.8
MS.gene012960.t1 MTR_5g076700 25.243 412 253 17 66 451 62 444 1.37e-21 97.4
MS.gene012960.t1 MTR_6g015815 26.501 483 275 20 5 443 6 452 2.14e-21 96.7
MS.gene012960.t1 MTR_7g114520 28.293 410 238 21 69 451 66 446 1.15e-20 94.7
MS.gene012960.t1 MTR_1g053315 24.582 419 271 17 14 415 26 416 2.52e-20 93.6
MS.gene012960.t1 MTR_6g053810 28.720 289 188 10 69 349 71 349 4.49e-20 92.8
MS.gene012960.t1 MTR_4g013050 25.862 290 165 9 67 350 51 296 1.61e-19 90.9
MS.gene012960.t1 MTR_3g071320 26.136 352 218 12 68 400 59 387 1.63e-19 90.9
MS.gene012960.t1 MTR_7g114470 25.362 414 245 19 69 451 66 446 4.06e-19 89.7
MS.gene012960.t1 MTR_1g040175 26.420 352 215 13 69 404 64 387 8.19e-19 89.0
MS.gene012960.t1 MTR_7g085990 25.349 430 260 20 55 444 42 450 2.08e-18 87.8
MS.gene012960.t1 MTR_7g114550 26.044 407 255 18 66 451 62 443 2.84e-18 87.4
MS.gene012960.t1 MTR_7g026830 51.429 70 33 1 113 181 28 97 3.33e-18 80.1
MS.gene012960.t1 MTR_6g015850 25.620 484 274 17 5 443 7 449 3.43e-18 87.0
MS.gene012960.t1 MTR_7g014330 25.791 411 240 16 29 402 26 408 6.36e-18 86.3
MS.gene012960.t1 MTR_3g465980 27.397 292 180 10 57 334 56 329 6.50e-18 86.3
MS.gene012960.t1 MTR_3g028210 24.760 416 268 14 55 440 53 453 7.27e-18 86.3
MS.gene012960.t1 MTR_8g012750 27.083 384 234 17 55 402 48 421 7.75e-18 86.3
MS.gene012960.t1 MTR_7g084910 28.358 268 161 8 67 320 68 318 9.35e-18 85.5
MS.gene012960.t1 MTR_7g114490 26.914 405 232 19 69 444 65 434 1.05e-17 85.9
MS.gene012960.t1 MTR_6g015830 24.711 433 262 13 46 443 49 452 1.34e-17 85.1
MS.gene012960.t1 MTR_3g465990 27.740 292 179 11 57 334 56 329 1.57e-17 85.1
MS.gene012960.t1 MTR_8g079970 26.359 368 226 17 55 406 46 384 1.66e-17 84.7
MS.gene012960.t1 MTR_1g040215 26.667 375 229 17 66 424 61 405 1.70e-17 85.1
MS.gene012960.t1 MTR_4g055170 26.879 346 216 11 3 334 9 331 1.75e-17 85.1
MS.gene012960.t1 MTR_3g450710 24.332 374 230 14 63 404 59 411 1.83e-17 84.7
MS.gene012960.t1 MTR_7g085930 25.622 402 232 16 55 412 46 424 5.09e-17 83.6
MS.gene012960.t1 MTR_1g081660 25.763 295 189 12 66 349 70 345 5.12e-17 83.6
MS.gene012960.t1 MTR_6g034485 37.391 115 71 1 209 322 1 115 5.99e-17 77.4
MS.gene012960.t1 MTR_3g465910 27.240 279 171 10 70 334 69 329 1.90e-15 79.0
MS.gene012960.t1 MTR_7g014230 26.150 413 236 17 29 402 28 410 2.09e-15 78.6
MS.gene012960.t1 MTR_3g465930 25.000 276 185 6 69 334 69 332 3.32e-15 77.8
MS.gene012960.t1 MTR_2g089765 24.429 438 236 19 23 402 33 433 5.51e-15 77.4
MS.gene012960.t1 MTR_5g090880 25.134 374 216 16 69 404 67 414 1.22e-14 76.3
MS.gene012960.t1 MTR_2g089755 24.122 427 244 17 29 406 37 432 2.36e-14 75.5
MS.gene012960.t1 MTR_7g056087 23.795 332 218 12 55 367 45 360 2.57e-14 75.1
MS.gene012960.t1 MTR_7g014250 26.056 426 237 19 19 402 19 408 2.76e-14 75.1
MS.gene012960.t1 MTR_0502s0010 30.992 242 142 11 113 342 2 230 4.67e-14 72.0
MS.gene012960.t1 MTR_3g450690 23.056 373 233 14 63 404 66 415 7.13e-14 73.9
MS.gene012960.t1 MTR_3g465880 26.866 268 167 10 69 321 68 321 1.16e-13 73.2
MS.gene012960.t1 MTR_3g465950 24.427 262 179 7 70 322 69 320 1.29e-13 73.2
MS.gene012960.t1 MTR_6g015315 24.880 418 251 16 61 443 55 444 1.32e-13 72.8
MS.gene012960.t1 MTR_5g090830 23.306 369 228 13 69 404 66 412 4.80e-13 71.2
MS.gene012960.t1 MTR_5g090810 23.035 369 229 13 69 404 66 412 7.15e-12 67.8
MS.gene012960.t1 MTR_2g089670 22.396 384 239 13 55 402 68 428 8.40e-12 67.4
MS.gene012960.t1 MTR_1g094790 26.596 282 157 10 62 322 53 305 3.62e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012960.t1 AT5G42830 53.167 442 199 3 13 453 14 448 5.69e-168 480
MS.gene012960.t1 AT5G07850 51.542 454 213 5 2 453 4 452 7.30e-162 465
MS.gene012960.t1 AT5G07860 48.673 452 221 6 4 453 6 448 6.70e-144 419
MS.gene012960.t1 AT5G07870 47.323 467 221 5 2 453 4 460 1.23e-142 416
MS.gene012960.t1 AT5G67150 38.961 462 243 13 8 453 7 445 1.89e-98 303
MS.gene012960.t1 AT2G39980 37.367 471 221 11 33 453 33 479 4.63e-94 292
MS.gene012960.t1 AT3G50270 35.760 467 266 10 1 453 1 447 9.61e-89 278
MS.gene012960.t1 AT5G01210 37.602 492 244 13 1 453 1 468 7.77e-88 276
MS.gene012960.t1 AT3G50280 36.080 449 264 9 9 450 7 439 1.15e-85 270
MS.gene012960.t1 AT3G50300 34.056 461 271 10 8 454 6 447 1.12e-79 254
MS.gene012960.t1 AT5G38130 36.009 436 252 13 8 435 26 442 8.97e-79 252
MS.gene012960.t1 AT5G67160 35.294 425 245 11 29 449 29 427 6.31e-73 236
MS.gene012960.t1 AT3G50280 38.730 315 182 4 141 450 1 309 3.74e-66 215
MS.gene012960.t1 AT5G23940 30.786 458 290 11 7 450 4 448 9.99e-59 200
MS.gene012960.t1 AT3G50290 40.769 260 142 5 149 401 15 269 5.16e-52 177
MS.gene012960.t1 AT5G41040 28.851 409 240 16 63 451 63 440 2.80e-36 139
MS.gene012960.t1 AT5G41040 28.851 409 240 16 63 451 79 456 3.58e-36 139
MS.gene012960.t1 AT2G19070 30.166 421 235 18 59 444 48 444 1.84e-35 137
MS.gene012960.t1 AT5G48930 27.404 416 244 13 55 444 44 427 3.65e-35 135
MS.gene012960.t1 AT5G63560 28.018 439 262 17 21 444 22 421 1.48e-30 122
MS.gene012960.t1 AT3G48720 28.019 414 250 14 47 444 44 425 2.66e-29 119
MS.gene012960.t1 AT5G57840 26.452 465 253 20 28 451 23 439 1.97e-24 105
MS.gene012960.t1 AT2G40230 27.838 370 222 13 64 414 54 397 3.06e-24 105
MS.gene012960.t1 AT1G31490 27.100 369 212 17 55 401 48 381 3.21e-21 96.3
MS.gene012960.t1 AT1G03390 26.260 377 236 15 54 415 64 413 1.84e-20 94.0
MS.gene012960.t1 AT3G29635 25.115 434 284 14 29 440 29 443 4.49e-20 92.8
MS.gene012960.t1 AT1G65450 29.195 298 172 14 63 340 66 344 5.34e-20 92.4
MS.gene012960.t1 AT1G27620 28.479 309 187 10 69 362 58 347 3.00e-19 90.1
MS.gene012960.t1 AT1G03940 24.146 410 256 16 56 427 53 445 5.94e-18 86.7
MS.gene012960.t1 AT5G39090 23.555 467 250 19 29 443 28 439 1.26e-16 82.4
MS.gene012960.t1 AT1G78990 24.586 362 219 14 67 404 65 396 1.33e-16 82.4
MS.gene012960.t1 AT3G29590 25.393 382 244 13 77 435 69 432 2.34e-16 81.6
MS.gene012960.t1 AT1G03495 27.928 333 193 17 29 331 30 345 2.68e-16 80.5
MS.gene012960.t1 AT1G32910 24.093 386 226 16 64 417 60 410 1.57e-15 79.0
MS.gene012960.t1 AT1G28680 26.821 302 192 12 72 362 65 348 1.99e-15 78.2
MS.gene012960.t1 AT1G28680 26.821 302 192 12 72 362 65 348 2.18e-15 78.6
MS.gene012960.t1 AT1G24420 25.322 466 286 19 6 452 5 427 3.19e-15 77.8
MS.gene012960.t1 AT1G24420 25.322 466 286 19 6 452 5 427 3.19e-15 77.8
MS.gene012960.t1 AT3G03480 28.125 320 183 18 43 342 46 338 1.07e-14 76.3
MS.gene012960.t1 AT3G26040 25.806 403 248 18 66 450 56 425 1.11e-14 76.3
MS.gene012960.t1 AT5G61160 24.537 432 263 18 46 446 41 440 2.63e-14 75.1
MS.gene012960.t1 AT2G25150 24.068 295 192 8 69 349 67 343 3.02e-14 75.1
MS.gene012960.t1 AT4G15390 26.398 322 195 14 7 320 9 296 1.20e-13 73.2
MS.gene012960.t1 AT4G15390 26.398 322 195 14 7 320 9 296 1.20e-13 73.2
MS.gene012960.t1 AT5G39050 23.385 325 199 11 155 443 149 459 2.04e-13 72.4
MS.gene012960.t1 AT5G47980 24.390 246 158 7 68 309 60 281 1.68e-11 66.2
MS.gene012960.t1 AT5G23970 24.878 410 255 18 56 450 46 417 3.55e-11 65.5

Find 104 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCGACGGCACCTCTTTC+TGG 0.148559 3.4:-89762743 MS.gene012960:CDS
TTCCATTTCCATCCTCTTTC+CGG 0.167351 3.4:-89763038 MS.gene012960:CDS
ATGAAATCTTGTTGGTTATT+TGG 0.171092 3.4:+89763107 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.211414 3.4:-89762096 MS.gene012960:CDS
CCTTTGCCACAAGAGTATTT+TGG 0.259472 3.4:-89762336 MS.gene012960:CDS
AAGCAAATTTGTTATTTAAC+AGG 0.266191 3.4:-89763200 MS.gene012960:CDS
CGTCGCCAATACAGTGATTC+AGG 0.271847 3.4:+89762757 None:intergenic
ATTTGCTTTGAATCTTCAAT+AGG 0.290643 3.4:+89763215 None:intergenic
TGTTTGAAGGGAAGGTTAAT+AGG 0.322567 3.4:+89762603 None:intergenic
ACTGAAAGTAAAGGCGAATA+AGG 0.331646 3.4:-89762499 MS.gene012960:CDS
AGATTTCATTGAGAATCTAT+TGG 0.350242 3.4:-89763093 MS.gene012960:CDS
GGAATTGCTTCAGAATAATC+TGG 0.355550 3.4:-89762274 MS.gene012960:CDS
TGAATCTTGCACCGGGGTTA+TGG 0.358731 3.4:+89762952 None:intergenic
CAATAGACTCCGGTGCAAAA+TGG 0.366621 3.4:+89762526 None:intergenic
TCACCAAGGTTCAATATGTA+TGG 0.368741 3.4:-89762108 MS.gene012960:CDS
TTTGCTTTGAATCTTCAATA+GGG 0.371311 3.4:+89763216 None:intergenic
AGGCTTTATCAGCACATGTT+TGG 0.374339 3.4:-89762446 MS.gene012960:CDS
GATTCTCAATGAAATCTTGT+TGG 0.378418 3.4:+89763099 None:intergenic
ATCAACGAAAATAGTATAAG+TGG 0.381161 3.4:+89762983 None:intergenic
ACTTGACACTGTGATACTTT+GGG 0.394360 3.4:+89762131 None:intergenic
GTGAAAAGGTAGACGCGAAA+TGG 0.400218 3.4:+89762656 None:intergenic
AACTTGACACTGTGATACTT+TGG 0.402547 3.4:+89762130 None:intergenic
TCAATGCACTACATCCAAAA+AGG 0.406674 3.4:-89763158 MS.gene012960:CDS
CCCTCATTGCATTAGGTGTA+AGG 0.407739 3.4:+89761968 None:intergenic
TATAAATAATCGACTAAGAT+TGG 0.409859 3.4:-89762364 MS.gene012960:CDS
TCCAACGCCCTCATTGCATT+AGG 0.417797 3.4:+89761961 None:intergenic
ATTTATAGCTAACTTACAAC+TGG 0.418010 3.4:+89762380 None:intergenic
TGAAAAGGTAGACGCGAAAT+GGG 0.427390 3.4:+89762657 None:intergenic
CACGATCGTTGGTTTCTACA+AGG 0.434379 3.4:-89762636 MS.gene012960:CDS
AAATTTGTTATTTAACAGGC+TGG 0.446269 3.4:-89763196 MS.gene012960:CDS
GGATATGCAAACAAATTTGA+TGG 0.446592 3.4:-89762045 MS.gene012960:CDS
TGAGCATGGAAGCATAGATT+TGG 0.460362 3.4:-89761998 MS.gene012960:CDS
AAACTCCTCATGATGTTTGA+AGG 0.463035 3.4:+89762590 None:intergenic
CGTTGGTTTCTACAAGGTTA+CGG 0.463520 3.4:-89762630 MS.gene012960:CDS
AAAGAGGATGGAAATGGAAA+AGG 0.466379 3.4:+89763042 None:intergenic
GGTAATGTATCATCATATCA+AGG 0.467204 3.4:-89762024 MS.gene012960:CDS
GGCCGGAAAGAGGATGGAAA+TGG 0.469243 3.4:+89763036 None:intergenic
CCAAAATACTCTTGTGGCAA+AGG 0.474802 3.4:+89762336 None:intergenic
ACAATTGGAGGAACATCAAC+CGG 0.476837 3.4:+89762888 None:intergenic
TGTTCCCTGAATCACTGTAT+TGG 0.487778 3.4:-89762762 MS.gene012960:CDS
CGGAGAGAGGATATCAGATA+TGG 0.489115 3.4:+89762908 None:intergenic
CGACCGAGTTGGTATACAAC+AGG 0.497202 3.4:+89762165 None:intergenic
CCTCATGATGTTTGAAGGGA+AGG 0.506492 3.4:+89762595 None:intergenic
AAGAATCTATCTCTCAAGTT+CGG 0.508887 3.4:+89762549 None:intergenic
TTCCCATACATATTGAACCT+TGG 0.519386 3.4:+89762105 None:intergenic
AATGGAAAAGGGCATGAGAG+AGG 0.519524 3.4:+89763054 None:intergenic
TCTGGGATGGGCAGCATGGA+AGG 0.526844 3.4:-89762256 MS.gene012960:CDS
TGCATAGATGAATCTTGCAC+CGG 0.532803 3.4:+89762944 None:intergenic
TGCTTCAGAATAATCTGGGA+TGG 0.534999 3.4:-89762269 MS.gene012960:CDS
AATTCAAGTCACAGAGCTAG+TGG 0.536764 3.4:-89762802 MS.gene012960:CDS
AAGAGGATGGAAATGGAAAA+GGG 0.539593 3.4:+89763043 None:intergenic
GAATCTTCAATAGGGTGTAG+TGG 0.541386 3.4:+89763224 None:intergenic
AGAAACCAACGATCGTGAAA+AGG 0.544715 3.4:+89762642 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.545045 3.4:-89762090 MS.gene012960:CDS
ACCTAATGCAATGAGGGCGT+TGG 0.546046 3.4:-89761962 MS.gene012960:CDS
ATGGAAAAGGGCATGAGAGA+GGG 0.546368 3.4:+89763055 None:intergenic
GTGACAAGACGGCCGGAAAG+AGG 0.547124 3.4:+89763026 None:intergenic
TATTGCAAAACTGAAAGTAA+AGG 0.547981 3.4:-89762508 MS.gene012960:CDS
CATATCTGATATCCTCTCTC+CGG 0.547986 3.4:-89762907 MS.gene012960:CDS
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.4:-89762107 MS.gene012960:CDS
AATTTGTTATTTAACAGGCT+GGG 0.563430 3.4:-89763195 MS.gene012960:CDS
AACTCCTCATGATGTTTGAA+GGG 0.567259 3.4:+89762591 None:intergenic
CGAAAATAGTATAAGTGGGA+GGG 0.567849 3.4:+89762988 None:intergenic
CAAGACGGCCGGAAAGAGGA+TGG 0.572452 3.4:+89763030 None:intergenic
GTCGCCAATACAGTGATTCA+GGG 0.572933 3.4:+89762758 None:intergenic
GAATTGCTTCAGAATAATCT+GGG 0.575502 3.4:-89762273 MS.gene012960:CDS
GCATAGATGAATCTTGCACC+GGG 0.575886 3.4:+89762945 None:intergenic
AAATACTCTTGTGGCAAAGG+TGG 0.579415 3.4:+89762339 None:intergenic
TCATATCAAGGGTATGAGCA+TGG 0.589477 3.4:-89762012 MS.gene012960:CDS
CCTTACACCTAATGCAATGA+GGG 0.589516 3.4:-89761968 MS.gene012960:CDS
TTTGTGAGTGACAAGACGGC+CGG 0.593267 3.4:+89763019 None:intergenic
CCTTCCCTTCAAACATCATG+AGG 0.593883 3.4:-89762595 MS.gene012960:CDS
GTATGGGAATGAATTTGGAA+TGG 0.594042 3.4:-89762091 MS.gene012960:CDS
TCAACGAAAATAGTATAAGT+GGG 0.595520 3.4:+89762984 None:intergenic
CCTCATTGCATTAGGTGTAA+GGG 0.599303 3.4:+89761969 None:intergenic
GCTTCAGAATAATCTGGGAT+GGG 0.600304 3.4:-89762268 MS.gene012960:CDS
CTTGACACTGTGATACTTTG+GGG 0.607315 3.4:+89762132 None:intergenic
GATCAAAGTGGCGACCGAGT+TGG 0.615671 3.4:+89762154 None:intergenic
ATAATCTGGGATGGGCAGCA+TGG 0.616155 3.4:-89762260 MS.gene012960:CDS
CCCTTACACCTAATGCAATG+AGG 0.616533 3.4:-89761969 MS.gene012960:CDS
GTGTTTCCAAAATACTCTTG+TGG 0.617301 3.4:+89762330 None:intergenic
CTGAATCACTGTATTGGCGA+CGG 0.619022 3.4:-89762756 MS.gene012960:CDS
CAAGTCACAGAGCTAGTGGA+TGG 0.621329 3.4:-89762798 MS.gene012960:CDS
ACGAAAATAGTATAAGTGGG+AGG 0.621520 3.4:+89762987 None:intergenic
GTAATGTATCATCATATCAA+GGG 0.623652 3.4:-89762023 MS.gene012960:CDS
ATGGAAGGTTAATGTGGCTG+TGG 0.624262 3.4:-89762241 MS.gene012960:CDS
GAGGAACATCAACCGGAGAG+AGG 0.629237 3.4:+89762895 None:intergenic
TTGAAGGGAAGGTTAATAGG+TGG 0.632790 3.4:+89762606 None:intergenic
TGATACTTTGGGGATCAAAG+TGG 0.645918 3.4:+89762142 None:intergenic
TCTCCTGTTGTATACCAACT+CGG 0.651878 3.4:-89762168 MS.gene012960:CDS
AGATGTGTCACACGTGCACG+CGG 0.655089 3.4:-89762423 MS.gene012960:CDS
GTGACTTGAATTGATAACAA+CGG 0.655100 3.4:+89762813 None:intergenic
GGCAGCATGGAAGGTTAATG+TGG 0.663013 3.4:-89762247 MS.gene012960:CDS
CGAGGAGTTCATGAATACTG+TGG 0.664706 3.4:-89761932 MS.gene012960:CDS
TAGTGCAGAAACGACTGTGG+AGG 0.677510 3.4:-89762295 MS.gene012960:CDS
GGTTAGTGCAGAAACGACTG+TGG 0.691168 3.4:-89762298 MS.gene012960:CDS
TTGAATTGATAACAACGGCA+TGG 0.701648 3.4:+89762818 None:intergenic
GATTGCGGTAACCATAACCC+CGG 0.707381 3.4:-89762963 MS.gene012960:CDS
AAAGCGATAGCAGTTCTGAG+TGG 0.710377 3.4:-89762066 MS.gene012960:CDS
AACAAAGCAATCAACCACGA+CGG 0.711672 3.4:-89762846 MS.gene012960:CDS
GAGGGCGTTGGAATTAGACG+AGG 0.733168 3.4:-89761950 MS.gene012960:CDS
ACGGCATGGTGTGACCGTCG+TGG 0.733894 3.4:+89762832 None:intergenic
TCACAGTGTCAAGTTCACCA+AGG 0.739939 3.4:-89762122 MS.gene012960:CDS
CATAGATGAATCTTGCACCG+GGG 0.755506 3.4:+89762946 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTGGAATTTTTTTAATATA+TGG - chr3.4:89762471-89762490 MS.gene012960:CDS 15.0%
!!! TTGTTTGTTTTTTGAATAAT+AGG + chr3.4:89762064-89762083 None:intergenic 15.0%
!! ATTAAAAAAATTCCAGAAAG+AGG + chr3.4:89762468-89762487 None:intergenic 20.0%
!! TATAAATAATCGACTAAGAT+TGG - chr3.4:89762832-89762851 MS.gene012960:CDS 20.0%
!!! AAGCAAATTTGTTATTTAAC+AGG - chr3.4:89761996-89762015 MS.gene012960:CDS 20.0%
! AACAAAAATATCTTCGTTTC+AGG - chr3.4:89762730-89762749 MS.gene012960:CDS 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.4:89763100-89763119 MS.gene012960:CDS 25.0%
! AGATTTCATTGAGAATCTAT+TGG - chr3.4:89762103-89762122 MS.gene012960:CDS 25.0%
! ATCAACGAAAATAGTATAAG+TGG + chr3.4:89762216-89762235 None:intergenic 25.0%
! ATTTATAGCTAACTTACAAC+TGG + chr3.4:89762819-89762838 None:intergenic 25.0%
! GTAATGTATCATCATATCAA+GGG - chr3.4:89763173-89763192 MS.gene012960:CDS 25.0%
! TATTGCAAAACTGAAAGTAA+AGG - chr3.4:89762688-89762707 MS.gene012960:CDS 25.0%
! TCAAAAAATGACTCAACAAT+TGG + chr3.4:89762326-89762345 None:intergenic 25.0%
! TCAACGAAAATAGTATAAGT+GGG + chr3.4:89762215-89762234 None:intergenic 25.0%
!! ATGAAATCTTGTTGGTTATT+TGG + chr3.4:89762092-89762111 None:intergenic 25.0%
!! ATTTGCTTTGAATCTTCAAT+AGG + chr3.4:89761984-89762003 None:intergenic 25.0%
!! TTTGCTTTGAATCTTCAATA+GGG + chr3.4:89761983-89762002 None:intergenic 25.0%
!! TTTTGAATAATAGGCCTTTT+TGG + chr3.4:89762055-89762074 None:intergenic 25.0%
!!! AAATTTGTTATTTAACAGGC+TGG - chr3.4:89762000-89762019 MS.gene012960:CDS 25.0%
!!! AATTTGTTATTTAACAGGCT+GGG - chr3.4:89762001-89762020 MS.gene012960:CDS 25.0%
!!! TATACTATTTTCGTTGATTG+CGG - chr3.4:89762218-89762237 MS.gene012960:CDS 25.0%
AAAAATGACTCAACAATTGG+AGG + chr3.4:89762323-89762342 None:intergenic 30.0%
AAGAATCTATCTCTCAAGTT+CGG + chr3.4:89762650-89762669 None:intergenic 30.0%
GAATTGCTTCAGAATAATCT+GGG - chr3.4:89762923-89762942 MS.gene012960:CDS 30.0%
GATTCTCAATGAAATCTTGT+TGG + chr3.4:89762100-89762119 None:intergenic 30.0%
GGATATGCAAACAAATTTGA+TGG - chr3.4:89763151-89763170 MS.gene012960:CDS 30.0%
GGTAATGTATCATCATATCA+AGG - chr3.4:89763172-89763191 MS.gene012960:CDS 30.0%
! GTGACTTGAATTGATAACAA+CGG + chr3.4:89762386-89762405 None:intergenic 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.4:89763106-89763125 MS.gene012960:CDS 30.0%
AAACTCCTCATGATGTTTGA+AGG + chr3.4:89762609-89762628 None:intergenic 35.0%
AAAGAGGATGGAAATGGAAA+AGG + chr3.4:89762157-89762176 None:intergenic 35.0%
AACTCCTCATGATGTTTGAA+GGG + chr3.4:89762608-89762627 None:intergenic 35.0%
AACTTGACACTGTGATACTT+TGG + chr3.4:89763069-89763088 None:intergenic 35.0%
AAGAGGATGGAAATGGAAAA+GGG + chr3.4:89762156-89762175 None:intergenic 35.0%
ACGAAAATAGTATAAGTGGG+AGG + chr3.4:89762212-89762231 None:intergenic 35.0%
ACTGAAAGTAAAGGCGAATA+AGG - chr3.4:89762697-89762716 MS.gene012960:CDS 35.0%
ACTTGACACTGTGATACTTT+GGG + chr3.4:89763068-89763087 None:intergenic 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.4:89763089-89763108 MS.gene012960:CDS 35.0%
CGAAAATAGTATAAGTGGGA+GGG + chr3.4:89762211-89762230 None:intergenic 35.0%
GGAATTGCTTCAGAATAATC+TGG - chr3.4:89762922-89762941 MS.gene012960:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.4:89763105-89763124 MS.gene012960:CDS 35.0%
GTGTTTCCAAAATACTCTTG+TGG + chr3.4:89762869-89762888 None:intergenic 35.0%
TCAATGCACTACATCCAAAA+AGG - chr3.4:89762038-89762057 MS.gene012960:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.4:89763088-89763107 MS.gene012960:CDS 35.0%
TGCGAAAGATGCTGAAAAAA+TGG - chr3.4:89762999-89763018 MS.gene012960:CDS 35.0%
TGTTTGAAGGGAAGGTTAAT+AGG + chr3.4:89762596-89762615 None:intergenic 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.4:89763094-89763113 None:intergenic 35.0%
! TAGATTCTTCCATTTTGCAC+CGG - chr3.4:89762661-89762680 MS.gene012960:CDS 35.0%
! TTCAGTTTTGCAATAGACTC+CGG + chr3.4:89762683-89762702 None:intergenic 35.0%
! TTGAATTGATAACAACGGCA+TGG + chr3.4:89762381-89762400 None:intergenic 35.0%
AAATACTCTTGTGGCAAAGG+TGG + chr3.4:89762860-89762879 None:intergenic 40.0%
AACAAAGCAATCAACCACGA+CGG - chr3.4:89762350-89762369 MS.gene012960:CDS 40.0%
AATTCAAGTCACAGAGCTAG+TGG - chr3.4:89762394-89762413 MS.gene012960:CDS 40.0%
ACAATTGGAGGAACATCAAC+CGG + chr3.4:89762311-89762330 None:intergenic 40.0%
AGAAACCAACGATCGTGAAA+AGG + chr3.4:89762557-89762576 None:intergenic 40.0%
CATATCTGATATCCTCTCTC+CGG - chr3.4:89762289-89762308 MS.gene012960:CDS 40.0%
CCAAAATACTCTTGTGGCAA+AGG + chr3.4:89762863-89762882 None:intergenic 40.0%
CCTCATTGCATTAGGTGTAA+GGG + chr3.4:89763230-89763249 None:intergenic 40.0%
CCTTACACCTAATGCAATGA+GGG - chr3.4:89763228-89763247 MS.gene012960:CDS 40.0%
CTTGACACTGTGATACTTTG+GGG + chr3.4:89763067-89763086 None:intergenic 40.0%
GAATCTTCAATAGGGTGTAG+TGG + chr3.4:89761975-89761994 None:intergenic 40.0%
TCTCCTGTTGTATACCAACT+CGG - chr3.4:89763028-89763047 MS.gene012960:CDS 40.0%
TGAAAAGGTAGACGCGAAAT+GGG + chr3.4:89762542-89762561 None:intergenic 40.0%
TGCATAGATGAATCTTGCAC+CGG + chr3.4:89762255-89762274 None:intergenic 40.0%
TGCTTCAGAATAATCTGGGA+TGG - chr3.4:89762927-89762946 MS.gene012960:CDS 40.0%
TGTTCCCTGAATCACTGTAT+TGG - chr3.4:89762434-89762453 MS.gene012960:CDS 40.0%
TTCCATTTCCATCCTCTTTC+CGG - chr3.4:89762158-89762177 MS.gene012960:CDS 40.0%
TTGAAGGGAAGGTTAATAGG+TGG + chr3.4:89762593-89762612 None:intergenic 40.0%
! AGGCTTTATCAGCACATGTT+TGG - chr3.4:89762750-89762769 MS.gene012960:CDS 40.0%
! GCTTCAGAATAATCTGGGAT+GGG - chr3.4:89762928-89762947 MS.gene012960:CDS 40.0%
! TCATATCAAGGGTATGAGCA+TGG - chr3.4:89763184-89763203 MS.gene012960:CDS 40.0%
! TGAGCATGGAAGCATAGATT+TGG - chr3.4:89763198-89763217 MS.gene012960:CDS 40.0%
! TGATACTTTGGGGATCAAAG+TGG + chr3.4:89763057-89763076 None:intergenic 40.0%
!! CGTTGGTTTCTACAAGGTTA+CGG - chr3.4:89762566-89762585 MS.gene012960:CDS 40.0%
!! CTAGTGGATGGTGTTTTCAT+AGG - chr3.4:89762410-89762429 MS.gene012960:CDS 40.0%
!! GAGTTTTGTGAGTGACAAGA+CGG + chr3.4:89762184-89762203 None:intergenic 40.0%
!! TTTTGGAAACACAGTTGACG+TGG - chr3.4:89762877-89762896 MS.gene012960:CDS 40.0%
!!! CCTTTGCCACAAGAGTATTT+TGG - chr3.4:89762860-89762879 MS.gene012960:CDS 40.0%
ATGGAAGGTTAATGTGGCTG+TGG - chr3.4:89762955-89762974 MS.gene012960:CDS 45.0%
CAATAGACTCCGGTGCAAAA+TGG + chr3.4:89762673-89762692 None:intergenic 45.0%
CATAGATGAATCTTGCACCG+GGG + chr3.4:89762253-89762272 None:intergenic 45.0%
CCCTCATTGCATTAGGTGTA+AGG + chr3.4:89763231-89763250 None:intergenic 45.0%
CCCTTACACCTAATGCAATG+AGG - chr3.4:89763227-89763246 MS.gene012960:CDS 45.0%
CCTCATGATGTTTGAAGGGA+AGG + chr3.4:89762604-89762623 None:intergenic 45.0%
CCTTCCCTTCAAACATCATG+AGG - chr3.4:89762601-89762620 MS.gene012960:CDS 45.0%
CGAGGAGTTCATGAATACTG+TGG - chr3.4:89763264-89763283 MS.gene012960:CDS 45.0%
CGGAGAGAGGATATCAGATA+TGG + chr3.4:89762291-89762310 None:intergenic 45.0%
GCATAGATGAATCTTGCACC+GGG + chr3.4:89762254-89762273 None:intergenic 45.0%
GTCGCCAATACAGTGATTCA+GGG + chr3.4:89762441-89762460 None:intergenic 45.0%
GTGAAAAGGTAGACGCGAAA+TGG + chr3.4:89762543-89762562 None:intergenic 45.0%
TCACAGTGTCAAGTTCACCA+AGG - chr3.4:89763074-89763093 MS.gene012960:CDS 45.0%
! AATGGAAAAGGGCATGAGAG+AGG + chr3.4:89762145-89762164 None:intergenic 45.0%
! ATGGAAAAGGGCATGAGAGA+GGG + chr3.4:89762144-89762163 None:intergenic 45.0%
! CTGAATCACTGTATTGGCGA+CGG - chr3.4:89762440-89762459 MS.gene012960:CDS 45.0%
! GTCTACCTTTTCACGATCGT+TGG - chr3.4:89762549-89762568 MS.gene012960:CDS 45.0%
!! AAAGCGATAGCAGTTCTGAG+TGG - chr3.4:89763130-89763149 MS.gene012960:CDS 45.0%
!! CACGATCGTTGGTTTCTACA+AGG - chr3.4:89762560-89762579 MS.gene012960:CDS 45.0%
CAAGTCACAGAGCTAGTGGA+TGG - chr3.4:89762398-89762417 MS.gene012960:CDS 50.0%
CGTCGCCAATACAGTGATTC+AGG + chr3.4:89762442-89762461 None:intergenic 50.0%
GATTGCGGTAACCATAACCC+CGG - chr3.4:89762233-89762252 MS.gene012960:CDS 50.0%
GGCAGCATGGAAGGTTAATG+TGG - chr3.4:89762949-89762968 MS.gene012960:CDS 50.0%
GGTTAGTGCAGAAACGACTG+TGG - chr3.4:89762898-89762917 MS.gene012960:CDS 50.0%
TAGTGCAGAAACGACTGTGG+AGG - chr3.4:89762901-89762920 MS.gene012960:CDS 50.0%
TCCAACGCCCTCATTGCATT+AGG + chr3.4:89763238-89763257 None:intergenic 50.0%
TGAATCTTGCACCGGGGTTA+TGG + chr3.4:89762247-89762266 None:intergenic 50.0%
TTTGTGAGTGACAAGACGGC+CGG + chr3.4:89762180-89762199 None:intergenic 50.0%
! ACCTAATGCAATGAGGGCGT+TGG - chr3.4:89763234-89763253 MS.gene012960:CDS 50.0%
! ATAATCTGGGATGGGCAGCA+TGG - chr3.4:89762936-89762955 MS.gene012960:CDS 50.0%
!! CGACCGAGTTGGTATACAAC+AGG + chr3.4:89763034-89763053 None:intergenic 50.0%
AGATGTGTCACACGTGCACG+CGG - chr3.4:89762773-89762792 MS.gene012960:CDS 55.0%
GAGGAACATCAACCGGAGAG+AGG + chr3.4:89762304-89762323 None:intergenic 55.0%
GATCAAAGTGGCGACCGAGT+TGG + chr3.4:89763045-89763064 None:intergenic 55.0%
GGCCGGAAAGAGGATGGAAA+TGG + chr3.4:89762163-89762182 None:intergenic 55.0%
!! GAGGGCGTTGGAATTAGACG+AGG - chr3.4:89763246-89763265 MS.gene012960:CDS 55.0%
CAAGACGGCCGGAAAGAGGA+TGG + chr3.4:89762169-89762188 None:intergenic 60.0%
GTGACAAGACGGCCGGAAAG+AGG + chr3.4:89762173-89762192 None:intergenic 60.0%
TTGGCGACGGCACCTCTTTC+TGG - chr3.4:89762453-89762472 MS.gene012960:CDS 60.0%
! TCTGGGATGGGCAGCATGGA+AGG - chr3.4:89762940-89762959 MS.gene012960:CDS 60.0%
ACGGCATGGTGTGACCGTCG+TGG + chr3.4:89762367-89762386 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89761927 89763291 89761927 ID=MS.gene012960
chr3.4 mRNA 89761927 89763291 89761927 ID=MS.gene012960.t1;Parent=MS.gene012960
chr3.4 exon 89761927 89763291 89761927 ID=MS.gene012960.t1.exon1;Parent=MS.gene012960.t1
chr3.4 CDS 89761927 89763291 89761927 ID=cds.MS.gene012960.t1;Parent=MS.gene012960.t1
Gene Sequence

>MS.gene012960

ATGACTTCTTCTAGCGCTCAAACAGTTTCAGAATGTTTTATCAAACCACTACACCCTATTGAAGATTCAAAGCAAATTTGTTATTTAACAGGCTGGGATATTGCTATGTCATCAATGCACTACATCCAAAAAGGCCTATTATTCAAAAAACAAACAACTTCACCAAATAACCAACAAGATTTCATTGAGAATCTATTGGAGAAGCTTAAACACTCCCTCTCTCATGCCCTTTTCCATTTCCATCCTCTTTCCGGCCGTCTTGTCACTCACAAAACTCAAAACCCTCCCACTTATACTATTTTCGTTGATTGCGGTAACCATAACCCCGGTGCAAGATTCATCTATGCAACTGCAAACGTGACCATATCTGATATCCTCTCTCCGGTTGATGTTCCTCCAATTGTTGAGTCATTTTTTGATCTCAACAAAGCAATCAACCACGACGGTCACACCATGCCGTTGTTATCAATTCAAGTCACAGAGCTAGTGGATGGTGTTTTCATAGGTTGTTCCCTGAATCACTGTATTGGCGACGGCACCTCTTTCTGGAATTTTTTTAATATATGGTCTGAGATATTTCAATCTCAAAGTTGTGAAAATGAAAACGATGTTCCCATTTCGCGTCTACCTTTTCACGATCGTTGGTTTCTACAAGGTTACGGTCCACCTATTAACCTTCCCTTCAAACATCATGAGGAGTTTATCAGCAGATATGAATCTCCGAACTTGAGAGATAGATTCTTCCATTTTGCACCGGAGTCTATTGCAAAACTGAAAGTAAAGGCGAATAAGGAGTTTAACACAACAAAAATATCTTCGTTTCAGGCTTTATCAGCACATGTTTGGAGATGTGTCACACGTGCACGCGGACTAACAAATGATCGAAAAACCAGTTGTAAGTTAGCTATAAATAATCGACTAAGATTGGAACCACCTTTGCCACAAGAGTATTTTGGAAACACAGTTGACGTGGTTAGTGCAGAAACGACTGTGGAGGAATTGCTTCAGAATAATCTGGGATGGGCAGCATGGAAGGTTAATGTGGCTGTGGTGAATCATGACGATAAAGAAGTGCGAAAGATGCTGAAAAAATGGTTAGAGTCTCCTGTTGTATACCAACTCGGTCGCCACTTTGATCCCCAAAGTATCACAGTGTCAAGTTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGTAAAGCGATAGCAGTTCTGAGTGGATATGCAAACAAATTTGATGGTAATGTATCATCATATCAAGGGTATGAGCATGGAAGCATAGATTTGGCGTTGACCCTTACACCTAATGCAATGAGGGCGTTGGAATTAGACGAGGAGTTCATGAATACTGTGGTTTGA
Protein sequence

>MS.gene012960.t1

MTSSSAQTVSECFIKPLHPIEDSKQICYLTGWDIAMSSMHYIQKGLLFKKQTTSPNNQQDFIENLLEKLKHSLSHALFHFHPLSGRLVTHKTQNPPTYTIFVDCGNHNPGARFIYATANVTISDILSPVDVPPIVESFFDLNKAINHDGHTMPLLSIQVTELVDGVFIGCSLNHCIGDGTSFWNFFNIWSEIFQSQSCENENDVPISRLPFHDRWFLQGYGPPINLPFKHHEEFISRYESPNLRDRFFHFAPESIAKLKVKANKEFNTTKISSFQALSAHVWRCVTRARGLTNDRKTSCKLAINNRLRLEPPLPQEYFGNTVDVVSAETTVEELLQNNLGWAAWKVNVAVVNHDDKEVRKMLKKWLESPVVYQLGRHFDPQSITVSSSPRFNMYGNEFGMGKAIAVLSGYANKFDGNVSSYQGYEHGSIDLALTLTPNAMRALELDEEFMNTVV