AlfalfaGEDB | MS.gene32934

M. sativa cultivar XinJiangDaYe / MS.gene32934

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32934.t1	XP_003603071.2	77.6	456	40	1	1	394	2	457	1.10E-200	709.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32934.t1	Q9SND9	30.4	441	240	10	6	384	4	439	5.3e-50	199.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32934.t1	G7J6X8	77.6	456	40	1	1	394	2	457	8.0e-201	709.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene32934	MS.gene000763	PPI
MS.gene32934	MS.gene018509	PPI
MS.gene32934	MS.gene28251	PPI
MS.gene32934	MS.gene41964	PPI
MS.gene32934	MS.gene90491	PPI
MS.gene32934	MS.gene38526	PPI
MS.gene071205	MS.gene32934	PPI
MS.gene32934	MS.gene50384	PPI
MS.gene32934	MS.gene066701	PPI
MS.gene32934	MS.gene50355	PPI
MS.gene32934	MS.gene99218	PPI
MS.gene32934	MS.gene22910	PPI
MS.gene32934	MS.gene89988	PPI
MS.gene32934	MS.gene99511	PPI
MS.gene32261	MS.gene32934	PPI
MS.gene78121	MS.gene32934	PPI
MS.gene32934	MS.gene000743	PPI
MS.gene32934	MS.gene41950	PPI
MS.gene32934	MS.gene064101	PPI
MS.gene59166	MS.gene32934	PPI
MS.gene32934	MS.gene050495	PPI
MS.gene99219	MS.gene32934	PPI
MS.gene32934	MS.gene038274	PPI
MS.gene32934	MS.gene50356	PPI
MS.gene90496	MS.gene32934	PPI
MS.gene32934	MS.gene87536	PPI
MS.gene32934	MS.gene56797	PPI
MS.gene32934	MS.gene071208	PPI
MS.gene56795	MS.gene32934	PPI
MS.gene024605	MS.gene32934	PPI
MS.gene32934	MS.gene07708	PPI
MS.gene066657	MS.gene32934	PPI
MS.gene017058	MS.gene32934	PPI
MS.gene32934	MS.gene78818	PPI
MS.gene32934	MS.gene38527	PPI
MS.gene32934	MS.gene69496	PPI
MS.gene41585	MS.gene32934	PPI
MS.gene32934	MS.gene016532	PPI
MS.gene50379	MS.gene32934	PPI
MS.gene071211	MS.gene32934	PPI
MS.gene38977	MS.gene32934	PPI
MS.gene32934	MS.gene054235	PPI
MS.gene99236	MS.gene32934	PPI
MS.gene026147	MS.gene32934	PPI
MS.gene32934	MS.gene019178	PPI
MS.gene90492	MS.gene32934	PPI
MS.gene32934	MS.gene011799	PPI
MS.gene021238	MS.gene32934	PPI
MS.gene32934	MS.gene50420	PPI
MS.gene32934	MS.gene22889	PPI
MS.gene011837	MS.gene32934	PPI
MS.gene066634	MS.gene32934	PPI
MS.gene59174	MS.gene32934	PPI
MS.gene32934	MS.gene054214	PPI
MS.gene32934	MS.gene50382	PPI
MS.gene32934	MS.gene99514	PPI
MS.gene38528	MS.gene32934	PPI
MS.gene32934	MS.gene47721	PPI
MS.gene32934	MS.gene99516	PPI
MS.gene32934	MS.gene049466	PPI
MS.gene32934	MS.gene050494	PPI
MS.gene063865	MS.gene32934	PPI
MS.gene32934	MS.gene060028	PPI
MS.gene48027	MS.gene32934	PPI
MS.gene058903	MS.gene32934	PPI
MS.gene32934	MS.gene87191	PPI
MS.gene32934	MS.gene99234	PPI
MS.gene011796	MS.gene32934	PPI
MS.gene011801	MS.gene32934	PPI
MS.gene32934	MS.gene016477	PPI
MS.gene87537	MS.gene32934	PPI
MS.gene000764	MS.gene32934	PPI
MS.gene32934	MS.gene017054	PPI
MS.gene32934	MS.gene02573	PPI
MS.gene90493	MS.gene32934	PPI
MS.gene41583	MS.gene32934	PPI
MS.gene017053	MS.gene32934	PPI
MS.gene013787	MS.gene32934	PPI
MS.gene87532	MS.gene32934	PPI
MS.gene99223	MS.gene32934	PPI
MS.gene32934	MS.gene87577	PPI
MS.gene46265	MS.gene32934	PPI
MS.gene29897	MS.gene32934	PPI
MS.gene063868	MS.gene32934	PPI
MS.gene47716	MS.gene32934	PPI
MS.gene026143	MS.gene32934	PPI
MS.gene026145	MS.gene32934	PPI
MS.gene99244	MS.gene32934	PPI
MS.gene32934	MS.gene038271	PPI
MS.gene99221	MS.gene32934	PPI
MS.gene32934	MS.gene026146	PPI
MS.gene03202	MS.gene32934	PPI
MS.gene008570	MS.gene32934	PPI
MS.gene32934	MS.gene41582	PPI
MS.gene32934	MS.gene56798	PPI
MS.gene007831	MS.gene32934	PPI
MS.gene32934	MS.gene41580	PPI
MS.gene008574	MS.gene32934	PPI
MS.gene32934	MS.gene008572	PPI
MS.gene48021	MS.gene32934	PPI
MS.gene56796	MS.gene32934	PPI
MS.gene32934	MS.gene071209	PPI
MS.gene32934	MS.gene027262	PPI
MS.gene32934	MS.gene22199	PPI
MS.gene48023	MS.gene32934	PPI
MS.gene32934	MS.gene59169	PPI
MS.gene026144	MS.gene32934	PPI
MS.gene32934	MS.gene066658	PPI
MS.gene32934	MS.gene87496	PPI
MS.gene32934	MS.gene070019	PPI
MS.gene32934	MS.gene99515	PPI
MS.gene99222	MS.gene32934	PPI
MS.gene38522	MS.gene32934	PPI
MS.gene011798	MS.gene32934	PPI
MS.gene32934	MS.gene47719	PPI
MS.gene032349	MS.gene32934	PPI
MS.gene066656	MS.gene32934	PPI
MS.gene32934	MS.gene024597	PPI
MS.gene066660	MS.gene32934	PPI
MS.gene32934	MS.gene56799	PPI
MS.gene38529	MS.gene32934	PPI
MS.gene024611	MS.gene32934	PPI
MS.gene050499	MS.gene32934	PPI
MS.gene32934	MS.gene000507	PPI
MS.gene32934	MS.gene59170	PPI
MS.gene32934	MS.gene069767	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32934.t1	MTR_3g102010	76.974	456	43	4	1	394	2	457	0.0	694
MS.gene32934.t1	MTR_3g101960	67.191	445	84	4	1	383	1	445	0.0	595
MS.gene32934.t1	MTR_3g101970	66.438	438	98	4	1	389	1	438	0.0	578
MS.gene32934.t1	MTR_3g101900	55.211	451	137	7	1	389	2	449	6.10e-166	473
MS.gene32934.t1	MTR_3g101930	55.286	454	135	9	1	387	2	454	7.84e-164	467
MS.gene32934.t1	MTR_3g102030	57.560	377	67	4	1	341	1	320	8.16e-143	409
MS.gene32934.t1	MTR_3g102020	77.253	233	44	3	1	228	1	229	8.79e-125	368
MS.gene32934.t1	MTR_3g102020	81.768	181	33	0	214	394	277	457	2.68e-104	316
MS.gene32934.t1	MTR_4g094838	35.556	405	208	8	29	384	28	428	1.53e-69	225
MS.gene32934.t1	MTR_8g041770	33.333	417	216	9	29	388	30	441	2.81e-66	217
MS.gene32934.t1	MTR_4g094835	34.679	421	208	9	25	384	28	442	7.68e-66	216
MS.gene32934.t1	MTR_0190s0020	33.890	419	215	9	28	388	28	442	1.70e-65	215
MS.gene32934.t1	MTR_4g022800	33.257	436	214	12	21	388	16	442	3.22e-62	206
MS.gene32934.t1	MTR_8g079710	34.383	413	200	11	21	369	21	426	1.41e-60	202
MS.gene32934.t1	MTR_4g022290	33.824	408	200	10	23	367	24	424	2.96e-60	201
MS.gene32934.t1	MTR_4g022670	31.954	435	220	10	21	388	16	441	4.61e-59	198
MS.gene32934.t1	MTR_4g022720	32.867	429	218	11	21	388	16	435	4.64e-59	198
MS.gene32934.t1	MTR_4g094842	31.767	447	231	13	6	384	4	444	1.34e-58	197
MS.gene32934.t1	MTR_7g026860	32.644	435	214	13	21	388	16	438	7.30e-56	190
MS.gene32934.t1	MTR_4g094825	33.609	363	175	8	29	388	92	391	4.21e-51	176
MS.gene32934.t1	MTR_3g070170	47.368	171	89	1	214	384	297	466	3.72e-49	173
MS.gene32934.t1	MTR_3g070170	35.472	265	139	8	1	243	1	255	1.64e-35	136
MS.gene32934.t1	MTR_4g094832	33.237	346	164	8	101	384	86	426	1.39e-47	167
MS.gene32934.t1	MTR_5g081960	46.199	171	91	1	214	384	313	482	2.28e-46	166
MS.gene32934.t1	MTR_5g081960	43.915	189	92	7	1	186	1	178	3.40e-34	132
MS.gene32934.t1	MTR_4g015180	43.750	176	98	1	213	388	313	487	1.25e-43	158
MS.gene32934.t1	MTR_4g015180	40.845	213	109	8	1	209	1	200	2.80e-33	130
MS.gene32934.t1	MTR_4g094828	40.566	212	103	7	6	211	4	198	3.60e-36	137
MS.gene32934.t1	MTR_4g094828	35.754	179	110	5	214	389	275	451	1.93e-21	95.9
MS.gene32934.t1	MTR_4g022760	35.931	231	124	7	21	239	16	234	1.38e-33	129
MS.gene32934.t1	MTR_6g016815	27.907	387	209	12	29	388	38	381	8.10e-32	124
MS.gene32934.t1	MTR_4g022825	40.000	185	97	5	29	212	24	195	1.26e-30	122
MS.gene32934.t1	MTR_4g022825	37.079	178	104	5	214	388	268	440	2.09e-27	113
MS.gene32934.t1	MTR_3g088420	36.872	179	109	3	211	388	258	433	4.25e-30	120
MS.gene32934.t1	MTR_3g088420	41.361	191	98	6	23	212	18	195	7.99e-29	117
MS.gene32934.t1	MTR_7g026850	46.207	145	71	5	43	186	21	159	1.08e-29	113
MS.gene32934.t1	MTR_6g045553	36.571	175	108	2	214	387	302	474	5.00e-28	115
MS.gene32934.t1	MTR_6g045553	28.022	182	119	4	6	186	14	184	6.45e-14	73.6
MS.gene32934.t1	MTR_3g026310	38.710	186	101	6	29	213	24	197	9.04e-27	111
MS.gene32934.t1	MTR_3g026310	38.122	181	106	5	211	388	271	448	5.87e-26	108
MS.gene32934.t1	MTR_8g040760	34.615	208	125	6	7	213	5	202	9.09e-26	103
MS.gene32934.t1	MTR_8g040780	32.418	182	118	4	214	391	56	236	3.26e-25	103
MS.gene32934.t1	MTR_6g034495	43.478	115	61	3	75	186	45	158	1.46e-21	91.3
MS.gene32934.t1	MTR_7g026830	47.500	80	40	2	97	175	19	97	1.13e-15	72.4
MS.gene32934.t1	MTR_2g064495	27.976	168	117	2	214	378	260	426	1.77e-15	78.2
MS.gene32934.t1	MTR_2g105330	27.976	168	117	2	214	378	260	426	3.85e-15	77.0
MS.gene32934.t1	MTR_4g127650	28.814	177	105	3	214	374	274	445	1.32e-13	72.4
MS.gene32934.t1	MTR_4g097090	28.144	167	109	3	214	369	274	440	1.05e-12	69.7
MS.gene32934.t1	MTR_8g075610	25.749	167	120	2	214	378	250	414	2.08e-12	68.6
MS.gene32934.t1	MTR_4g007540	25.000	168	122	2	214	378	261	427	2.62e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32934.t1	AT5G07860	44.820	444	180	8	6	387	8	448	8.54e-119	353
MS.gene32934.t1	AT5G07850	43.111	450	185	6	6	388	8	453	5.10e-117	348
MS.gene32934.t1	AT5G67150	34.222	450	223	10	6	389	5	447	1.10e-81	257
MS.gene32934.t1	AT5G42830	53.636	220	92	4	13	228	14	227	6.95e-69	224
MS.gene32934.t1	AT5G42830	56.818	176	76	0	214	389	275	450	1.60e-66	218
MS.gene32934.t1	AT3G50270	31.567	453	242	10	1	389	1	449	1.72e-68	223
MS.gene32934.t1	AT3G50280	31.519	441	235	10	6	384	4	439	2.43e-62	207
MS.gene32934.t1	AT5G07870	52.604	192	85	1	2	187	4	195	1.44e-61	206
MS.gene32934.t1	AT5G07870	52.571	175	83	0	214	388	287	461	4.96e-56	191
MS.gene32934.t1	AT3G50300	30.047	426	233	8	6	370	4	425	1.55e-59	200
MS.gene32934.t1	AT5G67160	31.206	423	211	14	29	389	29	433	1.20e-53	184
MS.gene32934.t1	AT5G38130	30.070	429	227	12	6	370	24	443	2.06e-50	176
MS.gene32934.t1	AT5G01210	46.591	176	93	1	213	388	295	469	5.67e-47	167
MS.gene32934.t1	AT5G01210	35.115	262	138	9	1	238	1	254	4.04e-29	118
MS.gene32934.t1	AT2G39980	45.714	175	94	1	214	388	307	480	8.67e-46	164
MS.gene32934.t1	AT2G39980	50.943	159	69	4	29	186	29	179	3.37e-41	152
MS.gene32934.t1	AT3G50280	31.683	303	146	6	142	384	8	309	2.03e-37	138
MS.gene32934.t1	AT5G23940	36.782	174	107	2	214	386	279	450	2.51e-25	107
MS.gene32934.t1	AT5G23940	29.323	266	161	8	6	260	3	252	5.47e-19	89.0
MS.gene32934.t1	AT3G50290	28.405	257	117	5	143	334	15	269	3.13e-22	95.9
MS.gene32934.t1	AT2G19070	31.034	174	103	3	214	374	271	440	3.41e-14	74.3
MS.gene32934.t1	AT5G48930	27.381	168	118	2	214	378	261	427	2.82e-13	71.2

Find 93 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTTCCTAAAGACTATTT+TGG	0.102514	3.1:-80968993	MS.gene32934:CDS
AACATGTATGGGAATGAATT+TGG	0.201238	3.1:-80968753	MS.gene32934:CDS
TCTTGAAAAGAAGGCCCTTT+TGG	0.296275	3.1:+80969786	None:intergenic
TCAAAGAACGACTTGTAAAT+TGG	0.296666	3.1:-80969045	MS.gene32934:CDS
TGATCCTTACTCTGTGATAA+TGG	0.310340	3.1:-80968793	MS.gene32934:CDS
TTGGTGATGGAACTGCTTAT+TGG	0.332359	3.1:-80969403	MS.gene32934:CDS
TCACCAAGGTTTAACATGTA+TGG	0.340932	3.1:-80968765	MS.gene32934:CDS
ACCAACCTATTCACAATAGA+TGG	0.341627	3.1:-80969304	MS.gene32934:CDS
GAATTCCCAAAATAGTCTTT+AGG	0.346933	3.1:+80968987	None:intergenic
TGAATAGGTTGGTGTGAAAT+AGG	0.352905	3.1:+80969314	None:intergenic
AGATGAAGAGTTCATGAATT+CGG	0.367038	3.1:-80968589	MS.gene32934:CDS
CAGAGTTGTGCGACAATTAG+TGG	0.368231	3.1:-80968862	MS.gene32934:CDS
ACCTGAGAAAATGATGTTGC+TGG	0.375240	3.1:-80968616	MS.gene32934:CDS
GGAAACCATCTATTGTGAAT+AGG	0.381041	3.1:+80969299	None:intergenic
ATTTGCTTTGAGTCTTCAAT+TGG	0.388102	3.1:+80969857	None:intergenic
AATGGAAAAGTGTGAGAGAA+AGG	0.389200	3.1:+80969711	None:intergenic
CAAGTCACTGAATTATTAGA+TGG	0.399474	3.1:-80969458	MS.gene32934:CDS
GGATATGCTAACAAATTTGA+TGG	0.406437	3.1:-80968702	MS.gene32934:CDS
ACAATTGGTGGAACATCAAT+GGG	0.407395	3.1:+80969548	None:intergenic
GTCTGCAAATTATATCCAAA+AGG	0.411777	3.1:-80969801	MS.gene32934:CDS
TACAAGTCGTTCTTTGATCA+TGG	0.412492	3.1:+80969051	None:intergenic
TGTTCCATGAACCACGCTAT+TGG	0.418751	3.1:-80969422	MS.gene32934:CDS
AACAATTGGTGGAACATCAA+TGG	0.423468	3.1:+80969547	None:intergenic
TTCCATTTCTATCCCCTCTC+AGG	0.425196	3.1:-80969695	MS.gene32934:CDS
ATCACCAATAGCGTGGTTCA+TGG	0.426862	3.1:+80969418	None:intergenic
ATAGTGTAAGAATGAGGGTC+TGG	0.439263	3.1:+80969650	None:intergenic
GATCCTTACTCTGTGATAAT+GGG	0.440468	3.1:-80968792	MS.gene32934:CDS
AACGACGACAAGGGAATTGC+TGG	0.442900	3.1:-80968943	MS.gene32934:CDS
AATTAGTGGAAGAGTGGTTA+TGG	0.443528	3.1:-80968848	MS.gene32934:CDS
GGCAGCATGGAAGATTCATT+TGG	0.449442	3.1:-80968904	MS.gene32934:CDS
GGTTGGTGTGAAATAGGAAC+AGG	0.454254	3.1:+80969320	None:intergenic
GAGACGGTTCCATTCTCGTT+CGG	0.454579	3.1:+80969012	None:intergenic
TTGATGTTCCACCAATTGTT+CGG	0.461529	3.1:-80969544	MS.gene32934:CDS
ATGTTGGTTTCTTGAAAAGA+AGG	0.461814	3.1:+80969777	None:intergenic
TAGTGTAAGAATGAGGGTCT+GGG	0.461817	3.1:+80969651	None:intergenic
GAGTCTTCAATTGGAGGGTA+AGG	0.463582	3.1:+80969866	None:intergenic
TGCTTTGAGTCTTCAATTGG+AGG	0.465416	3.1:+80969860	None:intergenic
AATATGCTATTTAGCACCTT+GGG	0.466491	3.1:-80969837	MS.gene32934:CDS
ACAAGGGAATTGCTGGAGAA+TGG	0.475211	3.1:-80968936	MS.gene32934:CDS
CTACCCATTATCACAGAGTA+AGG	0.475829	3.1:+80968789	None:intergenic
AGAGTTCATGAATTCGGTTT+CGG	0.476951	3.1:-80968583	MS.gene32934:CDS
CACCAAGGTTTAACATGTAT+GGG	0.478963	3.1:-80968764	MS.gene32934:CDS
AAATATGCTATTTAGCACCT+TGG	0.488698	3.1:-80969838	MS.gene32934:CDS
ATGGAAAAGTGTGAGAGAAA+GGG	0.500507	3.1:+80969712	None:intergenic
TATAAACAACCGAACGAGAA+TGG	0.505724	3.1:-80969021	MS.gene32934:CDS
TTCCCATACATGTTAAACCT+TGG	0.506983	3.1:+80968762	None:intergenic
CATGGTTGACTGCTCTATCA+TGG	0.512989	3.1:+80969510	None:intergenic
TATGGGAATGAATTTGGAAT+GGG	0.522099	3.1:-80968747	MS.gene32934:CDS
ACTTATGGTGGTATCTAAAG+TGG	0.522360	3.1:+80969583	None:intergenic
TCTGCAAATTATATCCAAAA+GGG	0.527217	3.1:-80969800	MS.gene32934:CDS
GTCTCCTACAGTGGTTCGAA+TGG	0.527384	3.1:-80968826	MS.gene32934:CDS
ATTTGGAATGGGAAAAGCAT+TGG	0.528592	3.1:-80968736	MS.gene32934:CDS
CTCCCACCTCCTTCTTGACC+AGG	0.534392	3.1:+80968663	None:intergenic
ATGAACCACGCTATTGGTGA+TGG	0.536345	3.1:-80969416	MS.gene32934:CDS
CACAATAGATGGTTTCCTGA+AGG	0.536832	3.1:-80969293	MS.gene32934:intron
GGAAAAGTAACATCATATCC+TGG	0.542782	3.1:-80968681	MS.gene32934:CDS
TGCTGGAGAATGGACTAGGA+TGG	0.552507	3.1:-80968926	MS.gene32934:CDS
GACCTGAGAGGGGATAGAAA+TGG	0.554535	3.1:+80969693	None:intergenic
GGTAACAAGACGACCTGAGA+GGG	0.558547	3.1:+80969682	None:intergenic
CTCATCAAAGACTAAAACAA+AGG	0.560969	3.1:+80968552	None:intergenic
GTATGGGAATGAATTTGGAA+TGG	0.561756	3.1:-80968748	MS.gene32934:CDS
ACATCATATCCTGGTCAAGA+AGG	0.563921	3.1:-80968672	MS.gene32934:CDS
GCTTTGAGTCTTCAATTGGA+GGG	0.569743	3.1:+80969861	None:intergenic
GAATTGCTGGAGAATGGACT+AGG	0.570746	3.1:-80968930	MS.gene32934:CDS
AGTGTAAGAATGAGGGTCTG+GGG	0.572728	3.1:+80969652	None:intergenic
GCTGGAGAATGGACTAGGAT+GGG	0.576643	3.1:-80968925	MS.gene32934:CDS
TATCCTGGTCAAGAAGGAGG+TGG	0.578330	3.1:-80968666	MS.gene32934:CDS
GATAGAGCAGTCAACCATGA+TGG	0.580149	3.1:-80969506	MS.gene32934:CDS
GTGGTTATGGTCTCCTACAG+TGG	0.588913	3.1:-80968835	MS.gene32934:CDS
AAATAGTCTTTAGGAAGAGA+CGG	0.591105	3.1:+80968996	None:intergenic
ACCATCTATTGTGAATAGGT+TGG	0.592072	3.1:+80969303	None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG	0.597903	3.1:-80968723	MS.gene32934:CDS
GATATAATTTGCAGACAACA+TGG	0.598066	3.1:+80969808	None:intergenic
TGTTGATTTAGAGATGATGT+TGG	0.598567	3.1:+80969761	None:intergenic
GTCGTTCTTTGATCATGGTG+TGG	0.603113	3.1:+80969056	None:intergenic
ATGGACTAGGATGGGCAGCA+TGG	0.605164	3.1:-80968917	MS.gene32934:CDS
TGCGACAATTAGTGGAAGAG+TGG	0.606479	3.1:-80968854	MS.gene32934:CDS
ATCCTGGTCAAGAAGGAGGT+GGG	0.618629	3.1:-80968665	MS.gene32934:CDS
TGAGTACTGAAACGACGACA+AGG	0.622315	3.1:-80968953	MS.gene32934:CDS
ATGTCCATTCGAACCACTGT+AGG	0.635665	3.1:+80968822	None:intergenic
GACAACATGGCAATATCCCA+AGG	0.637095	3.1:+80969821	None:intergenic
TCATATCCTGGTCAAGAAGG+AGG	0.649960	3.1:-80968669	MS.gene32934:CDS
AAGACATGGTGTGACCATCA+TGG	0.667161	3.1:+80969492	None:intergenic
AGTGGTTCGAATGGACATGT+TGG	0.668853	3.1:-80968817	MS.gene32934:CDS
GGAGAGGATATCACTTATGG+TGG	0.678914	3.1:+80969571	None:intergenic
CAGTTCCATCACCAATAGCG+TGG	0.680180	3.1:+80969411	None:intergenic
TGATAATGGGTAGTTCACCA+AGG	0.690999	3.1:-80968779	MS.gene32934:CDS
GGGTAACAAGACGACCTGAG+AGG	0.695039	3.1:+80969681	None:intergenic
GAGTACTGAAACGACGACAA+GGG	0.699661	3.1:-80968952	MS.gene32934:CDS
TTGAATAGACAACAAAGACA+TGG	0.705301	3.1:+80969478	None:intergenic
AATTGGTGGAACATCAATGG+GGG	0.711890	3.1:+80969550	None:intergenic
CAATTGGTGGAACATCAATG+GGG	0.723019	3.1:+80969549	None:intergenic
GTAACAAGACGACCTGAGAG+GGG	0.731044	3.1:+80969683	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTCTGAGATATTTGAAA+AGG	-	chr3.1:80969108-80969127	MS.gene32934:intron	20.0%
!	ACAAAAATAGTGTAAGAATG+AGG	+	chr3.1:80968833-80968852	None:intergenic	25.0%
!	CAAAAATAGTGTAAGAATGA+GGG	+	chr3.1:80968832-80968851	None:intergenic	25.0%
!	TCTGCAAATTATATCCAAAA+GGG	-	chr3.1:80968674-80968693	MS.gene32934:CDS	25.0%
!!	TTATTAGATGGTGTTTTCAT+AGG	-	chr3.1:80969028-80969047	MS.gene32934:CDS	25.0%
	AACATGTATGGGAATGAATT+TGG	-	chr3.1:80969721-80969740	MS.gene32934:CDS	30.0%
	AAGATTCTCTCTCTCAAAAT+GGG	+	chr3.1:80969271-80969290	None:intergenic	30.0%
	AGATGAAGAGTTCATGAATT+CGG	-	chr3.1:80969885-80969904	MS.gene32934:CDS	30.0%
	CAAGTCACTGAATTATTAGA+TGG	-	chr3.1:80969016-80969035	MS.gene32934:CDS	30.0%
	CTCTTCGTTTCAATCTTTAT+CGG	-	chr3.1:80969360-80969379	MS.gene32934:CDS	30.0%
	GAATTCCCAAAATAGTCTTT+AGG	+	chr3.1:80969490-80969509	None:intergenic	30.0%
	GGATATGCTAACAAATTTGA+TGG	-	chr3.1:80969772-80969791	MS.gene32934:CDS	30.0%
	GTCTGCAAATTATATCCAAA+AGG	-	chr3.1:80968673-80968692	MS.gene32934:CDS	30.0%
	GTTGATTGCAAAAATAGTCT+TGG	-	chr3.1:80968851-80968870	MS.gene32934:CDS	30.0%
	TCAAAAAATGACCGAACAAT+TGG	+	chr3.1:80968944-80968963	None:intergenic	30.0%
	TCAAAGAACGACTTGTAAAT+TGG	-	chr3.1:80969429-80969448	MS.gene32934:CDS	30.0%
	TTGAATAGACAACAAAGACA+TGG	+	chr3.1:80968999-80969018	None:intergenic	30.0%
!	AAATAGTCTTTAGGAAGAGA+CGG	+	chr3.1:80969481-80969500	None:intergenic	30.0%
!	AAATATGCTATTTAGCACCT+TGG	-	chr3.1:80968636-80968655	MS.gene32934:CDS	30.0%
!	AATATGCTATTTAGCACCTT+GGG	-	chr3.1:80968637-80968656	MS.gene32934:CDS	30.0%
!	ATTTGCTTTGAGTCTTCAAT+TGG	+	chr3.1:80968620-80968639	None:intergenic	30.0%
!	CTCTTCCTAAAGACTATTTT+GGG	-	chr3.1:80969482-80969501	MS.gene32934:CDS	30.0%
!	GATATAATTTGCAGACAACA+TGG	+	chr3.1:80968669-80968688	None:intergenic	30.0%
!	TATGGGAATGAATTTGGAAT+GGG	-	chr3.1:80969727-80969746	MS.gene32934:CDS	30.0%
!	TCTCTTCCTAAAGACTATTT+TGG	-	chr3.1:80969481-80969500	MS.gene32934:CDS	30.0%
!!	ATGTTGGTTTCTTGAAAAGA+AGG	+	chr3.1:80968700-80968719	None:intergenic	30.0%
!!	TGTTGATTTAGAGATGATGT+TGG	+	chr3.1:80968716-80968735	None:intergenic	30.0%
	AAAAATGACCGAACAATTGG+TGG	+	chr3.1:80968941-80968960	None:intergenic	35.0%
	AACAATTGGTGGAACATCAA+TGG	+	chr3.1:80968930-80968949	None:intergenic	35.0%
	AACTTGTCGTGATGTTTGAA+AGG	+	chr3.1:80969229-80969248	None:intergenic	35.0%
	AATCTTTATCGGCACTAGTT+TGG	-	chr3.1:80969371-80969390	MS.gene32934:CDS	35.0%
	AATTAGTGGAAGAGTGGTTA+TGG	-	chr3.1:80969626-80969645	MS.gene32934:CDS	35.0%
	ACAATTGGTGGAACATCAAT+GGG	+	chr3.1:80968929-80968948	None:intergenic	35.0%
	ACCAACCTATTCACAATAGA+TGG	-	chr3.1:80969170-80969189	MS.gene32934:intron	35.0%
	ACCATCTATTGTGAATAGGT+TGG	+	chr3.1:80969174-80969193	None:intergenic	35.0%
	ACTTATGGTGGTATCTAAAG+TGG	+	chr3.1:80968894-80968913	None:intergenic	35.0%
	ACTTGTCGTGATGTTTGAAA+GGG	+	chr3.1:80969228-80969247	None:intergenic	35.0%
	AGAGTTCATGAATTCGGTTT+CGG	-	chr3.1:80969891-80969910	MS.gene32934:CDS	35.0%
	ATAATTGGACCATAACCTTC+AGG	+	chr3.1:80969199-80969218	None:intergenic	35.0%
	CACCAAGGTTTAACATGTAT+GGG	-	chr3.1:80969710-80969729	MS.gene32934:CDS	35.0%
	GAAGATTCTCTCTCTCAAAA+TGG	+	chr3.1:80969272-80969291	None:intergenic	35.0%
	GATCCTTACTCTGTGATAAT+GGG	-	chr3.1:80969682-80969701	MS.gene32934:CDS	35.0%
	GGAAAAGTAACATCATATCC+TGG	-	chr3.1:80969793-80969812	MS.gene32934:CDS	35.0%
	GGAAACCATCTATTGTGAAT+AGG	+	chr3.1:80969178-80969197	None:intergenic	35.0%
	GTATGGGAATGAATTTGGAA+TGG	-	chr3.1:80969726-80969745	MS.gene32934:CDS	35.0%
	TACAAGTCGTTCTTTGATCA+TGG	+	chr3.1:80969426-80969445	None:intergenic	35.0%
	TATAAACAACCGAACGAGAA+TGG	-	chr3.1:80969453-80969472	MS.gene32934:CDS	35.0%
	TCACCAAGGTTTAACATGTA+TGG	-	chr3.1:80969709-80969728	MS.gene32934:CDS	35.0%
	TGATCCTTACTCTGTGATAA+TGG	-	chr3.1:80969681-80969700	MS.gene32934:CDS	35.0%
	TTCCCATACATGTTAAACCT+TGG	+	chr3.1:80969715-80969734	None:intergenic	35.0%
	TTGATGTTCCACCAATTGTT+CGG	-	chr3.1:80968930-80968949	MS.gene32934:CDS	35.0%
!	AATGGAAAAGTGTGAGAGAA+AGG	+	chr3.1:80968766-80968785	None:intergenic	35.0%
!	AGAAACGAGTTTTACACAGT+GGG	+	chr3.1:80968573-80968592	None:intergenic	35.0%
!	ATGGAAAAGTGTGAGAGAAA+GGG	+	chr3.1:80968765-80968784	None:intergenic	35.0%
!	ATTTGGAATGGGAAAAGCAT+TGG	-	chr3.1:80969738-80969757	MS.gene32934:CDS	35.0%
!!	TGAATAGGTTGGTGTGAAAT+AGG	+	chr3.1:80969163-80969182	None:intergenic	35.0%
!!	TTGAAAGGGAGGTTGATAAT+TGG	+	chr3.1:80969214-80969233	None:intergenic	35.0%
	AATTGGTGGAACATCAATGG+GGG	+	chr3.1:80968927-80968946	None:intergenic	40.0%
	ACATCATATCCTGGTCAAGA+AGG	-	chr3.1:80969802-80969821	MS.gene32934:CDS	40.0%
	ACCTGAGAAAATGATGTTGC+TGG	-	chr3.1:80969858-80969877	MS.gene32934:CDS	40.0%
	AGATGGTTTCCTGAAGGTTA+TGG	-	chr3.1:80969187-80969206	MS.gene32934:intron	40.0%
	ATAGTGTAAGAATGAGGGTC+TGG	+	chr3.1:80968827-80968846	None:intergenic	40.0%
	CAATTGGTGGAACATCAATG+GGG	+	chr3.1:80968928-80968947	None:intergenic	40.0%
	CACAATAGATGGTTTCCTGA+AGG	-	chr3.1:80969181-80969200	MS.gene32934:intron	40.0%
	CGATAGACTCTGCTGAAAAA+TGG	+	chr3.1:80969294-80969313	None:intergenic	40.0%
	CTACCCATTATCACAGAGTA+AGG	+	chr3.1:80969688-80969707	None:intergenic	40.0%
	TAGTGTAAGAATGAGGGTCT+GGG	+	chr3.1:80968826-80968845	None:intergenic	40.0%
!	CAGAAACGAGTTTTACACAG+TGG	+	chr3.1:80968574-80968593	None:intergenic	40.0%
!	GCTTTGAGTCTTCAATTGGA+GGG	+	chr3.1:80968616-80968635	None:intergenic	40.0%
!	TCCAGCAACATCATTTTCTC+AGG	+	chr3.1:80969862-80969881	None:intergenic	40.0%
!	TCTTGAAAAGAAGGCCCTTT+TGG	+	chr3.1:80968691-80968710	None:intergenic	40.0%
!	TGCTTTGAGTCTTCAATTGG+AGG	+	chr3.1:80968617-80968636	None:intergenic	40.0%
!!	AAAGCATTGGCAGTTAGAAG+TGG	-	chr3.1:80969751-80969770	MS.gene32934:CDS	40.0%
!!	TGATAATGGGTAGTTCACCA+AGG	-	chr3.1:80969695-80969714	MS.gene32934:CDS	40.0%
!!	TTGGTGATGGAACTGCTTAT+TGG	-	chr3.1:80969071-80969090	MS.gene32934:CDS	40.0%
	AAGACATGGTGTGACCATCA+TGG	+	chr3.1:80968985-80969004	None:intergenic	45.0%
	ACAAGGGAATTGCTGGAGAA+TGG	-	chr3.1:80969538-80969557	MS.gene32934:CDS	45.0%
	AGTGGTTCGAATGGACATGT+TGG	-	chr3.1:80969657-80969676	MS.gene32934:CDS	45.0%
	AGTGTAAGAATGAGGGTCTG+GGG	+	chr3.1:80968825-80968844	None:intergenic	45.0%
	ATCACCAATAGCGTGGTTCA+TGG	+	chr3.1:80969059-80969078	None:intergenic	45.0%
	ATGAACCACGCTATTGGTGA+TGG	-	chr3.1:80969058-80969077	MS.gene32934:CDS	45.0%
	ATGTCCATTCGAACCACTGT+AGG	+	chr3.1:80969655-80969674	None:intergenic	45.0%
	CAGAGTTGTGCGACAATTAG+TGG	-	chr3.1:80969612-80969631	MS.gene32934:CDS	45.0%
	CATGGTTGACTGCTCTATCA+TGG	+	chr3.1:80968967-80968986	None:intergenic	45.0%
	GACAACATGGCAATATCCCA+AGG	+	chr3.1:80968656-80968675	None:intergenic	45.0%
	GAGTCTTCAATTGGAGGGTA+AGG	+	chr3.1:80968611-80968630	None:intergenic	45.0%
	GATAGAGCAGTCAACCATGA+TGG	-	chr3.1:80968968-80968987	MS.gene32934:CDS	45.0%
	GGAGAGGATATCACTTATGG+TGG	+	chr3.1:80968906-80968925	None:intergenic	45.0%
	GGCAGCATGGAAGATTCATT+TGG	-	chr3.1:80969570-80969589	MS.gene32934:CDS	45.0%
	GTCGTTCTTTGATCATGGTG+TGG	+	chr3.1:80969421-80969440	None:intergenic	45.0%
	TCATATCCTGGTCAAGAAGG+AGG	-	chr3.1:80969805-80969824	MS.gene32934:CDS	45.0%
	TGCGACAATTAGTGGAAGAG+TGG	-	chr3.1:80969620-80969639	MS.gene32934:CDS	45.0%
	TGTCGTGATGTTTGAAAGGG+AGG	+	chr3.1:80969225-80969244	None:intergenic	45.0%
	TGTTCCATGAACCACGCTAT+TGG	-	chr3.1:80969052-80969071	MS.gene32934:CDS	45.0%
	TTCCATTTCTATCCCCTCTC+AGG	-	chr3.1:80968779-80968798	MS.gene32934:CDS	45.0%
!	GAATTGCTGGAGAATGGACT+AGG	-	chr3.1:80969544-80969563	MS.gene32934:CDS	45.0%
!	GGTTGGTGTGAAATAGGAAC+AGG	+	chr3.1:80969157-80969176	None:intergenic	45.0%
!!	GAGTACTGAAACGACGACAA+GGG	-	chr3.1:80969522-80969541	MS.gene32934:CDS	45.0%
!!	TGAGTACTGAAACGACGACA+AGG	-	chr3.1:80969521-80969540	MS.gene32934:CDS	45.0%
!!!	AATGAGGGTCTGGGGTTTTT+TGG	+	chr3.1:80968817-80968836	None:intergenic	45.0%
!!!	ATGAGGGTCTGGGGTTTTTT+GGG	+	chr3.1:80968816-80968835	None:intergenic	45.0%
	AACGACGACAAGGGAATTGC+TGG	-	chr3.1:80969531-80969550	MS.gene32934:CDS	50.0%
	ATCCTGGTCAAGAAGGAGGT+GGG	-	chr3.1:80969809-80969828	MS.gene32934:CDS	50.0%
	CAGTTCCATCACCAATAGCG+TGG	+	chr3.1:80969066-80969085	None:intergenic	50.0%
	GACCTGAGAGGGGATAGAAA+TGG	+	chr3.1:80968784-80968803	None:intergenic	50.0%
	GAGACGGTTCCATTCTCGTT+CGG	+	chr3.1:80969465-80969484	None:intergenic	50.0%
	GGGGGAGAGGATATCACTTA+TGG	+	chr3.1:80968909-80968928	None:intergenic	50.0%
	GGTAACAAGACGACCTGAGA+GGG	+	chr3.1:80968795-80968814	None:intergenic	50.0%
	GTAACAAGACGACCTGAGAG+GGG	+	chr3.1:80968794-80968813	None:intergenic	50.0%
	GTCTCCTACAGTGGTTCGAA+TGG	-	chr3.1:80969648-80969667	MS.gene32934:CDS	50.0%
	GTGGTTATGGTCTCCTACAG+TGG	-	chr3.1:80969639-80969658	MS.gene32934:CDS	50.0%
	TATCCTGGTCAAGAAGGAGG+TGG	-	chr3.1:80969808-80969827	MS.gene32934:CDS	50.0%
!!	GCTGGAGAATGGACTAGGAT+GGG	-	chr3.1:80969549-80969568	MS.gene32934:CDS	50.0%
!!	TGCTGGAGAATGGACTAGGA+TGG	-	chr3.1:80969548-80969567	MS.gene32934:CDS	50.0%
	GGGTAACAAGACGACCTGAG+AGG	+	chr3.1:80968796-80968815	None:intergenic	55.0%
	GTGGAACATCAATGGGGGAG+AGG	+	chr3.1:80968922-80968941	None:intergenic	55.0%
!!	ATGGACTAGGATGGGCAGCA+TGG	-	chr3.1:80969557-80969576	MS.gene32934:CDS	55.0%
	CTCCCACCTCCTTCTTGACC+AGG	+	chr3.1:80969814-80969833	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	80968563	80969933	80968563	ID=MS.gene32934
chr3.1	mRNA	80968563	80969933	80968563	ID=MS.gene32934.t1;Parent=MS.gene32934
chr3.1	exon	80969294	80969933	80969294	ID=MS.gene32934.t1.exon1;Parent=MS.gene32934.t1
chr3.1	CDS	80969294	80969933	80969294	ID=cds.MS.gene32934.t1;Parent=MS.gene32934.t1
chr3.1	exon	80968563	80969107	80968563	ID=MS.gene32934.t1.exon2;Parent=MS.gene32934.t1
chr3.1	CDS	80968563	80969107	80968563	ID=cds.MS.gene32934.t1;Parent=MS.gene32934.t1

Gene Sequence

>MS.gene32934

ATGAGCACCCACTGTGTAAAACTCGTTTCTGAATGCTTTATCAAACCTTACCCTCCAATTGAAGACTCAAAGCAAATATGCTATTTAGCACCTTGGGATATTGCCATGTTGTCTGCAAATTATATCCAAAAGGGCCTTCTTTTCAAGAAACCAACATCATCTCTAAATCAACAATATTTCATTGACAAACTCAAACACTCCCTTTCTCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACCCAAAAAACCCCAGACCCTCATTCTTACACTATTTTTGTTGATTGCAAAAATAGTCTTGGAGCTAAATTTCGTCATGCCACTTTAGATACCACCATAAGTGATATCCTCTCCCCCATTGATGTTCCACCAATTGTTCGGTCATTTTTTGACCATGATAGAGCAGTCAACCATGATGGTCACACCATGTCTTTGTTGTCTATTCAAGTCACTGAATTATTAGATGGTGTTTTCATAGGTTGTTCCATGAACCACGCTATTGGTGATGGAACTGCTTATTGGAATTTCTTTAACACATTTTCTGAGATATTTGAAAAGGATGTTGATGTTGATGTTCATATTCCTGTTCCTATTTCACACCAACCTATTCACAATAGATGGTTTCCTGAAGGTTATGGTCCAATTATCAACCTCCCTTTCAAACATCACGACAAGTTTATTGATAGATATGAAGCACCCATTTTGAGAGAGAGAATCTTCCATTTTTCAGCAGAGTCTATCGCGAAACTTAAAGCAAAAGCAAACAAAGAGAGCAAGACAACGCAAATCTCTTCGTTTCAATCTTTATCGGCACTAGTTTGGAGATCTATAACTCGTGTGCGTCAACCACACCATGATCAAAGAACGACTTGTAAATTGGCTATAAACAACCGAACGAGAATGGAACCGTCTCTTCCTAAAGACTATTTTGGGAATTCAATCTACGCAGTGAGTACTGAAACGACGACAAGGGAATTGCTGGAGAATGGACTAGGATGGGCAGCATGGAAGATTCATTTGGCTATTGCAAAATATGATCACAGAGTTGTGCGACAATTAGTGGAAGAGTGGTTATGGTCTCCTACAGTGGTTCGAATGGACATGTTGGTTGATCCTTACTCTGTGATAATGGGTAGTTCACCAAGGTTTAACATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCTGGTCAAGAAGGAGGTGGGAGCGTTGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCGGTTTCGGTGTTTAATCCTTTGTTTTAG

Protein sequence

>MS.gene32934.t1

MSTHCVKLVSECFIKPYPPIEDSKQICYLAPWDIAMLSANYIQKGLLFKKPTSSLNQQYFIDKLKHSLSLTLFHFYPLSGRLVTQKTPDPHSYTIFVDCKNSLGAKFRHATLDTTISDILSPIDVPPIVRSFFDHDRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHAIGDGTAYWNFFNTFSEIFEKDVDVDVHIPVPISHQPIHNRWFPEVWRSITRVRQPHHDQRTTCKLAINNRTRMEPSLPKDYFGNSIYAVSTETTTRELLENGLGWAAWKIHLAIAKYDHRVVRQLVEEWLWSPTVVRMDMLVDPYSVIMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGQEGGGSVDLEVCLSPEKMMLLETDEEFMNSVSVFNPLF