AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32933.t1 MTR_3g102020 94.541 458 24 1 1 458 1 457 0.0 901
MS.gene32933.t1 MTR_3g101960 88.367 447 50 1 1 447 1 445 0.0 837
MS.gene32933.t1 MTR_3g102010 84.199 462 63 3 1 458 2 457 0.0 806
MS.gene32933.t1 MTR_3g101970 83.664 453 59 2 1 453 1 438 0.0 797
MS.gene32933.t1 MTR_3g101900 67.621 454 140 3 1 453 2 449 0.0 632
MS.gene32933.t1 MTR_3g101930 66.154 455 148 4 1 451 2 454 0.0 622
MS.gene32933.t1 MTR_3g102030 79.245 318 40 2 88 405 29 320 0.0 522
MS.gene32933.t1 MTR_3g070170 39.959 493 216 15 2 448 8 466 1.69e-105 322
MS.gene32933.t1 MTR_5g081960 39.761 503 227 13 1 448 1 482 4.82e-100 308
MS.gene32933.t1 MTR_4g015180 39.328 506 234 13 1 452 1 487 1.28e-99 307
MS.gene32933.t1 MTR_8g041770 38.864 440 249 10 16 452 19 441 4.76e-93 288
MS.gene32933.t1 MTR_4g094842 39.216 459 249 11 4 448 2 444 1.96e-92 287
MS.gene32933.t1 MTR_4g022800 38.322 441 241 11 25 452 20 442 9.69e-90 280
MS.gene32933.t1 MTR_4g094835 38.979 431 240 8 25 448 28 442 1.11e-88 278
MS.gene32933.t1 MTR_0190s0020 38.228 429 247 8 28 452 28 442 3.32e-88 276
MS.gene32933.t1 MTR_4g094838 38.462 429 229 7 28 448 27 428 6.37e-87 273
MS.gene32933.t1 MTR_4g022825 38.182 440 243 10 24 452 19 440 8.95e-87 272
MS.gene32933.t1 MTR_3g088420 38.303 436 241 10 24 452 19 433 1.70e-86 271
MS.gene32933.t1 MTR_4g022720 38.710 434 244 9 24 452 19 435 2.03e-86 271
MS.gene32933.t1 MTR_4g022670 37.500 440 247 9 24 452 19 441 1.79e-84 266
MS.gene32933.t1 MTR_7g026860 36.818 440 247 10 24 452 19 438 6.43e-82 260
MS.gene32933.t1 MTR_3g026310 37.613 444 238 12 29 452 24 448 2.31e-80 256
MS.gene32933.t1 MTR_4g094828 37.753 445 241 13 26 453 26 451 8.49e-79 252
MS.gene32933.t1 MTR_4g022290 37.292 421 230 11 24 431 25 424 5.78e-78 249
MS.gene32933.t1 MTR_8g079710 38.915 424 222 17 25 433 25 426 9.07e-74 239
MS.gene32933.t1 MTR_4g094832 41.834 349 188 8 107 448 86 426 1.75e-72 235
MS.gene32933.t1 MTR_4g022760 36.340 388 210 11 24 388 19 392 3.52e-67 220
MS.gene32933.t1 MTR_6g045553 32.025 484 276 12 2 451 10 474 1.15e-61 208
MS.gene32933.t1 MTR_6g016815 30.858 431 214 11 24 452 33 381 7.99e-48 169
MS.gene32933.t1 MTR_8g040780 35.714 224 139 4 236 455 14 236 7.16e-39 141
MS.gene32933.t1 MTR_8g096530 31.646 316 176 11 64 360 53 347 1.27e-33 132
MS.gene32933.t1 MTR_8g075610 27.411 394 239 14 70 442 47 414 9.68e-33 129
MS.gene32933.t1 MTR_2g064495 27.013 385 255 13 70 442 56 426 3.56e-32 127
MS.gene32933.t1 MTR_2g105330 26.753 385 256 13 70 442 56 426 1.31e-31 125
MS.gene32933.t1 MTR_4g007540 26.904 394 245 13 70 442 56 427 1.41e-31 125
MS.gene32933.t1 MTR_3g098980 30.421 309 188 9 64 360 60 353 2.51e-31 125
MS.gene32933.t1 MTR_7g026850 41.401 157 79 4 43 198 21 165 2.64e-29 113
MS.gene32933.t1 MTR_3g049150 26.813 455 260 17 22 442 25 440 3.54e-29 119
MS.gene32933.t1 MTR_8g040760 34.978 223 132 5 4 225 2 212 6.15e-29 113
MS.gene32933.t1 MTR_4g127650 27.612 402 234 14 66 433 62 440 1.89e-27 114
MS.gene32933.t1 MTR_3g026950 24.788 472 269 19 17 447 20 446 2.20e-27 114
MS.gene32933.t1 MTR_7g026770 37.288 177 90 3 157 333 132 287 5.36e-27 110
MS.gene32933.t1 MTR_4g097090 27.295 403 235 15 66 433 61 440 1.04e-25 109
MS.gene32933.t1 MTR_4g094825 39.333 150 80 2 303 452 253 391 2.23e-25 107
MS.gene32933.t1 MTR_4g094825 34.706 170 98 4 29 195 92 251 1.85e-21 96.3
MS.gene32933.t1 MTR_5g076700 26.056 426 274 17 33 438 27 431 2.35e-25 108
MS.gene32933.t1 MTR_7g027175 30.159 315 194 10 58 360 56 356 1.16e-24 106
MS.gene32933.t1 MTR_6g034495 43.220 118 63 3 81 195 45 161 3.90e-23 96.3
MS.gene32933.t1 MTR_1g081660 26.573 429 275 16 29 439 31 437 4.89e-23 101
MS.gene32933.t1 MTR_3g112180 26.506 415 243 16 14 401 11 390 3.06e-22 99.4
MS.gene32933.t1 MTR_6g053810 31.359 287 179 12 70 346 71 349 3.43e-22 99.4
MS.gene32933.t1 MTR_7g114550 26.087 437 283 19 33 449 27 443 3.97e-22 99.0
MS.gene32933.t1 MTR_7g114520 26.697 442 278 20 33 450 28 447 1.48e-21 97.4
MS.gene32933.t1 MTR_3g071320 27.200 375 233 13 4 360 3 355 2.59e-21 96.3
MS.gene32933.t1 MTR_7g114490 26.637 443 283 19 33 455 27 447 2.73e-21 96.7
MS.gene32933.t1 MTR_7g086000 27.136 398 238 19 73 435 63 443 3.66e-21 96.3
MS.gene32933.t1 MTR_1g040175 27.248 367 230 17 67 421 61 402 7.28e-21 95.1
MS.gene32933.t1 MTR_1g040195 24.590 427 278 17 48 452 16 420 1.57e-20 94.0
MS.gene32933.t1 MTR_7g114560 25.858 437 284 19 33 449 27 443 2.51e-19 90.5
MS.gene32933.t1 MTR_3g465980 27.055 292 189 10 54 333 52 331 8.33e-19 89.0
MS.gene32933.t1 MTR_4g055170 26.619 278 177 8 70 333 69 333 9.13e-19 89.0
MS.gene32933.t1 MTR_2g015430 25.676 296 202 9 73 360 61 346 1.73e-18 87.8
MS.gene32933.t1 MTR_1g040215 28.522 291 188 11 67 349 61 339 4.61e-18 86.7
MS.gene32933.t1 MTR_6g034485 35.200 125 78 2 207 329 2 125 2.01e-17 78.6
MS.gene32933.t1 MTR_3g465990 26.370 292 191 10 54 333 52 331 7.84e-17 82.8
MS.gene32933.t1 MTR_6g015850 25.349 430 261 15 46 443 50 451 9.44e-17 82.8
MS.gene32933.t1 MTR_3g465930 27.500 280 177 9 53 318 52 319 1.88e-16 81.6
MS.gene32933.t1 MTR_7g084940 24.038 312 206 10 66 362 65 360 2.53e-16 81.3
MS.gene32933.t1 MTR_3g465950 27.742 310 199 9 27 323 27 324 2.96e-16 81.3
MS.gene32933.t1 MTR_4g013050 23.559 399 235 16 68 442 51 403 8.85e-16 79.3
MS.gene32933.t1 MTR_6g015830 24.408 422 278 14 46 443 50 454 1.09e-15 79.3
MS.gene32933.t1 MTR_5g026320 24.757 412 251 18 64 447 68 448 1.16e-15 79.3
MS.gene32933.t1 MTR_7g114960 24.747 396 273 10 67 448 60 444 6.11e-15 77.0
MS.gene32933.t1 MTR_7g026830 50.000 68 33 1 113 179 28 95 2.42e-14 68.9
MS.gene32933.t1 MTR_7g014620 23.864 440 282 21 29 443 15 426 2.84e-14 75.1
MS.gene32933.t1 MTR_0502s0010 31.250 240 141 11 113 339 2 230 3.78e-14 72.4
MS.gene32933.t1 MTR_3g049140 25.980 204 133 5 244 433 27 226 4.88e-14 72.4
MS.gene32933.t1 MTR_7g084910 25.283 265 173 7 66 317 66 318 2.81e-13 72.0
MS.gene32933.t1 MTR_7g085990 23.284 408 264 15 67 438 52 446 3.02e-13 72.0
MS.gene32933.t1 MTR_3g028210 23.617 470 298 18 6 438 8 453 4.95e-13 71.2
MS.gene32933.t1 MTR_6g015315 25.061 411 264 19 55 438 48 441 1.05e-12 70.1
MS.gene32933.t1 MTR_7g085930 23.399 406 258 14 66 433 55 445 1.08e-12 70.1
MS.gene32933.t1 MTR_8g079970 25.588 340 212 15 6 332 3 314 1.32e-12 69.7
MS.gene32933.t1 MTR_2g045540 32.955 88 51 1 244 331 27 106 1.19e-11 62.0
MS.gene32933.t1 MTR_7g014200 23.753 421 278 14 15 404 16 424 1.80e-11 66.2
MS.gene32933.t1 MTR_5g090830 24.013 304 190 14 65 344 61 347 8.54e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32933.t1 AT5G42830 56.532 444 183 2 13 453 14 450 0.0 528
MS.gene32933.t1 AT5G07850 53.982 452 205 2 2 452 4 453 0.0 515
MS.gene32933.t1 AT5G07860 53.778 450 199 4 4 451 6 448 1.98e-170 487
MS.gene32933.t1 AT5G07870 50.649 462 213 2 2 452 4 461 3.72e-166 476
MS.gene32933.t1 AT2G39980 40.169 473 213 8 29 452 29 480 5.87e-106 323
MS.gene32933.t1 AT5G67150 39.207 454 255 10 8 453 7 447 3.78e-99 305
MS.gene32933.t1 AT5G01210 39.431 492 235 13 1 452 1 469 9.64e-95 294
MS.gene32933.t1 AT3G50270 34.979 466 273 12 1 453 1 449 3.65e-82 261
MS.gene32933.t1 AT3G50280 35.099 453 271 11 4 448 2 439 1.98e-79 253
MS.gene32933.t1 AT3G50300 33.713 439 264 9 6 434 4 425 3.12e-75 243
MS.gene32933.t1 AT5G67160 34.884 430 252 11 28 453 28 433 9.19e-72 233
MS.gene32933.t1 AT5G38130 34.247 438 261 12 6 434 24 443 5.11e-71 232
MS.gene32933.t1 AT5G23940 33.839 461 276 13 6 450 3 450 2.71e-64 215
MS.gene32933.t1 AT3G50280 38.111 307 179 6 148 448 8 309 6.09e-58 194
MS.gene32933.t1 AT3G50290 37.452 259 149 6 149 398 15 269 2.27e-43 154
MS.gene32933.t1 AT2G19070 29.412 442 254 18 29 438 25 440 3.40e-35 136
MS.gene32933.t1 AT5G48930 25.546 458 287 16 6 442 3 427 2.07e-32 128
MS.gene32933.t1 AT5G41040 29.526 359 206 15 64 401 63 395 3.79e-32 127
MS.gene32933.t1 AT5G41040 29.526 359 206 15 64 401 79 411 4.46e-32 127
MS.gene32933.t1 AT3G48720 26.316 418 256 17 18 422 18 396 6.72e-23 100
MS.gene32933.t1 AT5G63560 28.125 320 195 12 53 360 49 345 1.79e-22 99.8
MS.gene32933.t1 AT2G40230 27.105 380 208 14 65 411 54 397 2.81e-22 99.4
MS.gene32933.t1 AT1G65450 27.245 323 200 12 58 360 60 367 1.14e-21 97.4
MS.gene32933.t1 AT1G28680 26.689 296 198 11 73 360 65 349 2.67e-21 95.9
MS.gene32933.t1 AT1G28680 26.689 296 198 11 73 360 65 349 4.19e-21 95.9
MS.gene32933.t1 AT5G57840 26.190 420 252 16 46 438 39 427 2.04e-20 94.0
MS.gene32933.t1 AT3G26040 26.582 474 281 23 6 456 3 432 4.08e-19 89.7
MS.gene32933.t1 AT5G07080 25.806 279 171 11 71 331 71 331 1.27e-15 79.3
MS.gene32933.t1 AT2G25150 27.000 300 187 12 63 346 60 343 6.33e-15 77.0
MS.gene32933.t1 AT1G32910 22.930 314 196 13 48 344 52 336 2.26e-14 75.5
MS.gene32933.t1 AT1G78990 23.390 295 197 10 61 344 57 333 9.51e-14 73.6
MS.gene32933.t1 AT1G24420 25.800 469 296 19 5 458 4 435 1.22e-13 73.2
MS.gene32933.t1 AT1G24420 25.800 469 296 19 5 458 4 435 1.22e-13 73.2
MS.gene32933.t1 AT3G03480 25.532 329 200 15 46 357 56 356 1.30e-13 73.2
MS.gene32933.t1 AT1G03390 24.566 403 255 18 55 433 64 441 2.47e-13 72.4
MS.gene32933.t1 AT1G03940 22.727 418 278 18 58 443 54 458 3.45e-13 71.6
MS.gene32933.t1 AT5G39090 22.944 462 305 16 5 441 4 439 7.24e-13 70.9
MS.gene32933.t1 AT3G29590 22.365 389 256 13 77 438 68 437 2.71e-12 68.9
MS.gene32933.t1 AT4G15390 23.765 324 214 12 3 321 5 300 9.47e-11 63.9
MS.gene32933.t1 AT4G15390 23.765 324 214 12 3 321 5 300 9.47e-11 63.9

Find 100 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.1:-80971462 MS.gene32933:CDS
CATTCAAGTTACTGAATTAT+TGG 0.137906 3.1:-80971919 MS.gene32933:CDS
AATATGTATGGGAATGAATT+TGG 0.204491 3.1:-80971222 MS.gene32933:CDS
AATGTGATAAATGGCATATT+TGG 0.269841 3.1:+80972315 None:intergenic
AATATTCTCTCTCTCAAAAT+AGG 0.288986 3.1:+80971675 None:intergenic
TAATATCCCAATGTGATAAA+TGG 0.297305 3.1:+80972306 None:intergenic
AATCTTTATCAGCACTAGTT+TGG 0.302845 3.1:-80971572 MS.gene32933:CDS
TCACCAAGGTTTAATATGTA+TGG 0.306646 3.1:-80971234 MS.gene32933:CDS
CATTCTCGTTCGGTTGTTTA+CGG 0.328680 3.1:+80971491 None:intergenic
CTACCCATCATCACAGTATA+AGG 0.338076 3.1:+80971258 None:intergenic
GGAAACCATCTATTGTGAAT+AGG 0.358486 3.1:+80971768 None:intergenic
ATAAACCTATTCACAATAGA+TGG 0.359431 3.1:-80971773 MS.gene32933:CDS
TCAACGAACAACTTGCAAAT+TGG 0.359564 3.1:-80971514 MS.gene32933:CDS
TCAAAGAATGATTGAACAAT+AGG 0.361615 3.1:+80971990 None:intergenic
ATGATTGGACCATAACCTTC+TGG 0.364910 3.1:+80971747 None:intergenic
AACAACAGCAGGGGAATTGC+TGG 0.367373 3.1:-80971412 MS.gene32933:CDS
ACCTGAGAAAATGATGTTGC+TGG 0.375240 3.1:-80971085 MS.gene32933:CDS
TGAATAGGTTTATGTGAAAT+CGG 0.391414 3.1:+80971783 None:intergenic
TTGGTGATGGAACTGGTTAT+TGG 0.394500 3.1:-80971860 MS.gene32933:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3.1:+80971875 None:intergenic
GGATATGCTAACAAATTTGA+TGG 0.406437 3.1:-80971171 MS.gene32933:CDS
TTCACCACTCACAGCGTAGA+TGG 0.412187 3.1:+80971434 None:intergenic
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.1:-80971756 MS.gene32933:CDS
CTTCGTGATGTTTGAAAGGA+AGG 0.418279 3.1:+80971721 None:intergenic
TTCCATTTCTATCCCCTCTC+AGG 0.425196 3.1:-80972152 MS.gene32933:CDS
ACAATAGGTGGCACATCAAC+AGG 0.427451 3.1:+80972005 None:intergenic
AATATGCCATTTATCACATT+GGG 0.437912 3.1:-80972312 MS.gene32933:CDS
AGTGTTTCAGTTTCTCTACT+AGG 0.438345 3.1:+80972192 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3.1:+80972213 None:intergenic
ATTTGGTTCAAGTCTTCGAT+TGG 0.440400 3.1:+80972332 None:intergenic
TGATCCTTATACTGTGATGA+TGG 0.442813 3.1:-80971262 MS.gene32933:CDS
GCGTGCACGTCAGTTACAAC+AGG 0.443295 3.1:-80971538 MS.gene32933:CDS
TTTGGTTCAAGTCTTCGATT+GGG 0.445265 3.1:+80972333 None:intergenic
CATTGTATTGGTGATGGAAC+TGG 0.455215 3.1:-80971867 MS.gene32933:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.1:+80972109 None:intergenic
AATGGAAAAGTGTGAGGGAA+AGG 0.463707 3.1:+80972168 None:intergenic
CGTAAACAACCGAACGAGAA+TGG 0.464267 3.1:-80971490 MS.gene32933:CDS
TTGAAAGGAAGGTTGATGAT+TGG 0.467597 3.1:+80971732 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.1:+80971462 None:intergenic
GAGGTGGTTCCATTCTCGTT+CGG 0.474421 3.1:+80971481 None:intergenic
TGATAAAGTGGTGCGAAATT+CGG 0.474906 3.1:-80971334 MS.gene32933:CDS
TTTAGTGATGATGATGATGT+TGG 0.478634 3.1:+80972236 None:intergenic
CATCATAATTTGCAATAGCC+AGG 0.479956 3.1:+80971355 None:intergenic
GAATTGCTGGAAAATGATAT+AGG 0.483480 3.1:-80971399 MS.gene32933:CDS
CTTCCTCCTCCTTCTTGACC+AGG 0.483660 3.1:+80971132 None:intergenic
CAATAATTCAGTAACTTGAA+TGG 0.486762 3.1:+80971920 None:intergenic
GAGTTCCCAAAATATTCCTT+AGG 0.486855 3.1:+80971456 None:intergenic
ATGGAAAAGTGTGAGGGAAA+GGG 0.490102 3.1:+80972169 None:intergenic
AAATATGCCATTTATCACAT+TGG 0.490158 3.1:-80972313 MS.gene32933:CDS
GGTTTATGTGAAATCGGAAC+AGG 0.490426 3.1:+80971789 None:intergenic
ATCTGCATACTATATCCAAA+AGG 0.491658 3.1:-80972276 MS.gene32933:CDS
TGTTCCATGAATCATTGTAT+TGG 0.493564 3.1:-80971879 MS.gene32933:CDS
CACCAAGGTTTAATATGTAT+GGG 0.497351 3.1:-80971233 MS.gene32933:CDS
TTCCCATACATATTAAACCT+TGG 0.501336 3.1:+80971231 None:intergenic
TGCTGGAAAATGATATAGGA+TGG 0.502278 3.1:-80971395 MS.gene32933:CDS
AGGTGAGAGAATATCATTGA+TGG 0.504727 3.1:+80972025 None:intergenic
CAAGTTACTGAATTATTGGA+TGG 0.511663 3.1:-80971915 MS.gene32933:CDS
AACTCTTCGTGATGTTTGAA+AGG 0.512389 3.1:+80971717 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.1:-80971216 MS.gene32933:CDS
AGATCCATTCGATACACAAA+AGG 0.526309 3.1:+80971291 None:intergenic
ATTTGGAATGGGAAAAGCAT+TGG 0.528592 3.1:-80971205 MS.gene32933:CDS
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.1:-80971873 MS.gene32933:CDS
GCATGAATAAATTTAGCTCC+AGG 0.532002 3.1:+80972062 None:intergenic
GGAAAAGTAACATCATATCC+TGG 0.542782 3.1:-80971150 MS.gene32933:CDS
CACAGAGCTGTCAACCATGA+TGG 0.543528 3.1:-80971963 MS.gene32933:CDS
CACAATAGATGGTTTCCAGA+AGG 0.548245 3.1:-80971762 MS.gene32933:CDS
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.1:+80972139 None:intergenic
ATTGTCAAAGATTAGAACAA+AGG 0.552804 3.1:+80971021 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.1:+80972150 None:intergenic
TGCAAATTATGATGATAAAG+TGG 0.556314 3.1:-80971346 MS.gene32933:CDS
TCTGCATACTATATCCAAAA+GGG 0.562084 3.1:-80972275 MS.gene32933:CDS
ACATCATATCCTGGTCAAGA+AGG 0.563921 3.1:-80971141 MS.gene32933:CDS
ATGATATAGGATGGGCAGCA+TGG 0.564359 3.1:-80971386 MS.gene32933:CDS
AATGGAACCACCTCTTCCTA+AGG 0.571584 3.1:-80971472 MS.gene32933:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.1:-80971217 MS.gene32933:CDS
GTGAGTGGTGAAACAACAGC+AGG 0.584213 3.1:-80971423 MS.gene32933:CDS
TGATAAAACATTCAGAAACA+AGG 0.589803 3.1:+80972366 None:intergenic
GATCCTTATACTGTGATGAT+GGG 0.590324 3.1:-80971261 MS.gene32933:CDS
GGCAGCATGGAAGATTCACC+TGG 0.593449 3.1:-80971373 MS.gene32933:CDS
AAGAATGATTGAACAATAGG+TGG 0.594549 3.1:+80971993 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.1:+80971465 None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG 0.597903 3.1:-80971192 MS.gene32933:CDS
GCTGGAAAATGATATAGGAT+GGG 0.602886 3.1:-80971394 MS.gene32933:CDS
TTGGTTCAAGTCTTCGATTG+GGG 0.606286 3.1:+80972334 None:intergenic
TGCGAAATTCGGTTGAAGAG+TGG 0.613912 3.1:-80971323 MS.gene32933:CDS
GATAGAAATGGAAAAGTGTG+AGG 0.622911 3.1:+80972162 None:intergenic
AACTCCATCTACGCTGTGAG+TGG 0.636071 3.1:-80971438 MS.gene32933:CDS
AAGACATGGTGTGTCCATCA+TGG 0.638582 3.1:+80971949 None:intergenic
TCATATCCTGGTCAAGAAGG+AGG 0.641492 3.1:-80971138 MS.gene32933:CDS
TATCCTGGTCAAGAAGGAGG+AGG 0.643667 3.1:-80971135 MS.gene32933:CDS
TGGTTCAAGTCTTCGATTGG+GGG 0.657471 3.1:+80972335 None:intergenic
TGAGTGGTGAAACAACAGCA+GGG 0.669868 3.1:-80971422 MS.gene32933:CDS
TTGAATGGACAACAAAGACA+TGG 0.674790 3.1:+80971935 None:intergenic
ATAGAAATGGAAAAGTGTGA+GGG 0.681846 3.1:+80972163 None:intergenic
CATCATGGTTGACAGCTCTG+TGG 0.688486 3.1:+80971964 None:intergenic
GAGTGGTGAAACAACAGCAG+GGG 0.692267 3.1:-80971421 MS.gene32933:CDS
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.1:+80972138 None:intergenic
TGATGATGGGTAGTTCACCA+AGG 0.700661 3.1:-80971248 MS.gene32933:CDS
CGTGCACGTCAGTTACAACA+GGG 0.707992 3.1:-80971537 MS.gene32933:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.1:+80972140 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGTATGGGAATGAATT+TGG - chr3.1:80972196-80972215 MS.gene32933:CDS 25.0%
! AATATTCTCTCTCTCAAAAT+AGG + chr3.1:80971746-80971765 None:intergenic 25.0%
! AATGTGATAAATGGCATATT+TGG + chr3.1:80971106-80971125 None:intergenic 25.0%
! ATAAACCTATTCACAATAGA+TGG - chr3.1:80971645-80971664 MS.gene32933:CDS 25.0%
! CAATAATTCAGTAACTTGAA+TGG + chr3.1:80971501-80971520 None:intergenic 25.0%
! TAATATCCCAATGTGATAAA+TGG + chr3.1:80971115-80971134 None:intergenic 25.0%
! TCAAAGAATGATTGAACAAT+AGG + chr3.1:80971431-80971450 None:intergenic 25.0%
! TGAATAGGTTTATGTGAAAT+CGG + chr3.1:80971638-80971657 None:intergenic 25.0%
! TGATAAAACATTCAGAAACA+AGG + chr3.1:80971055-80971074 None:intergenic 25.0%
! TGCAAATTATGATGATAAAG+TGG - chr3.1:80972072-80972091 MS.gene32933:CDS 25.0%
!! AAATATGCCATTTATCACAT+TGG - chr3.1:80971105-80971124 MS.gene32933:CDS 25.0%
!! AATATGCCATTTATCACATT+GGG - chr3.1:80971106-80971125 MS.gene32933:CDS 25.0%
!! CATTCAAGTTACTGAATTAT+TGG - chr3.1:80971499-80971518 MS.gene32933:CDS 25.0%
!!! TTATTGGATGGTGTTTTTAT+AGG - chr3.1:80971515-80971534 MS.gene32933:CDS 25.0%
AAGAATGATTGAACAATAGG+TGG + chr3.1:80971428-80971447 None:intergenic 30.0%
AATCTTTATCAGCACTAGTT+TGG - chr3.1:80971846-80971865 MS.gene32933:CDS 30.0%
ACAAAAACAGTGTAAGAATG+AGG + chr3.1:80971320-80971339 None:intergenic 30.0%
ATCACCAATACAATGATTCA+TGG + chr3.1:80971546-80971565 None:intergenic 30.0%
ATCTGCATACTATATCCAAA+AGG - chr3.1:80971142-80971161 MS.gene32933:CDS 30.0%
CAAGTTACTGAATTATTGGA+TGG - chr3.1:80971503-80971522 MS.gene32933:CDS 30.0%
CACCAAGGTTTAATATGTAT+GGG - chr3.1:80972185-80972204 MS.gene32933:CDS 30.0%
GAATTGCTGGAAAATGATAT+AGG - chr3.1:80972019-80972038 MS.gene32933:CDS 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.1:80972247-80972266 MS.gene32933:CDS 30.0%
TCACCAAGGTTTAATATGTA+TGG - chr3.1:80972184-80972203 MS.gene32933:CDS 30.0%
TCTGCATACTATATCCAAAA+GGG - chr3.1:80971143-80971162 MS.gene32933:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.1:80971539-80971558 MS.gene32933:CDS 30.0%
TTCCCATACATATTAAACCT+TGG + chr3.1:80972190-80972209 None:intergenic 30.0%
! ATAGAAATGGAAAAGTGTGA+GGG + chr3.1:80971258-80971277 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.1:80971545-80971564 MS.gene32933:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.1:80971957-80971976 MS.gene32933:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.1:80972202-80972221 MS.gene32933:CDS 30.0%
! TTTAGTGATGATGATGATGT+TGG + chr3.1:80971185-80971204 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.1:80971956-80971975 None:intergenic 35.0%
AACTCTTCGTGATGTTTGAA+AGG + chr3.1:80971704-80971723 None:intergenic 35.0%
AGATCCATTCGATACACAAA+AGG + chr3.1:80972130-80972149 None:intergenic 35.0%
AGGTGAGAGAATATCATTGA+TGG + chr3.1:80971396-80971415 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.1:80971312-80971331 None:intergenic 35.0%
AGTGTTTCAGTTTCTCTACT+AGG + chr3.1:80971229-80971248 None:intergenic 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.1:80971959-80971978 None:intergenic 35.0%
GAGTTCCCAAAATATTCCTT+AGG + chr3.1:80971965-80971984 None:intergenic 35.0%
GATCCTTATACTGTGATGAT+GGG - chr3.1:80972157-80972176 MS.gene32933:CDS 35.0%
GCTGGAAAATGATATAGGAT+GGG - chr3.1:80972024-80972043 MS.gene32933:CDS 35.0%
GGAAAAGTAACATCATATCC+TGG - chr3.1:80972268-80972287 MS.gene32933:CDS 35.0%
GGAAACCATCTATTGTGAAT+AGG + chr3.1:80971653-80971672 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.1:80971208-80971227 None:intergenic 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.1:80972201-80972220 MS.gene32933:CDS 35.0%
TCAACGAACAACTTGCAAAT+TGG - chr3.1:80971904-80971923 MS.gene32933:CDS 35.0%
TGATAAAGTGGTGCGAAATT+CGG - chr3.1:80972084-80972103 MS.gene32933:CDS 35.0%
TGATCCTTATACTGTGATGA+TGG - chr3.1:80972156-80972175 MS.gene32933:CDS 35.0%
TGCTGGAAAATGATATAGGA+TGG - chr3.1:80972023-80972042 MS.gene32933:CDS 35.0%
TTGAATGGACAACAAAGACA+TGG + chr3.1:80971486-80971505 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCAT+TGG - chr3.1:80972213-80972232 MS.gene32933:CDS 35.0%
! ATTTGGTTCAAGTCTTCGAT+TGG + chr3.1:80971089-80971108 None:intergenic 35.0%
! CATCATAATTTGCAATAGCC+AGG + chr3.1:80972066-80972085 None:intergenic 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.1:80971956-80971975 MS.gene32933:CDS 35.0%
! GATAGAAATGGAAAAGTGTG+AGG + chr3.1:80971259-80971278 None:intergenic 35.0%
! GCATGAATAAATTTAGCTCC+AGG + chr3.1:80971359-80971378 None:intergenic 35.0%
! TTCTCCTTTTGTGTATCGAA+TGG - chr3.1:80972123-80972142 MS.gene32933:CDS 35.0%
! TTTGGTTCAAGTCTTCGATT+GGG + chr3.1:80971088-80971107 None:intergenic 35.0%
!! TTGAAAGGAAGGTTGATGAT+TGG + chr3.1:80971689-80971708 None:intergenic 35.0%
!! TTTTGAAGAGAAGACCCTTT+TGG + chr3.1:80971160-80971179 None:intergenic 35.0%
ACATCATATCCTGGTCAAGA+AGG - chr3.1:80972277-80972296 MS.gene32933:CDS 40.0%
ACCTGAGAAAATGATGTTGC+TGG - chr3.1:80972333-80972352 MS.gene32933:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.1:80971662-80971681 MS.gene32933:CDS 40.0%
ATGATTGGACCATAACCTTC+TGG + chr3.1:80971674-80971693 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.1:80971656-80971675 MS.gene32933:CDS 40.0%
CATTCTCGTTCGGTTGTTTA+CGG + chr3.1:80971930-80971949 None:intergenic 40.0%
CTACCCATCATCACAGTATA+AGG + chr3.1:80972163-80972182 None:intergenic 40.0%
CTTCGTGATGTTTGAAAGGA+AGG + chr3.1:80971700-80971719 None:intergenic 40.0%
GGTTTATGTGAAATCGGAAC+AGG + chr3.1:80971632-80971651 None:intergenic 40.0%
TTGGTTCAAGTCTTCGATTG+GGG + chr3.1:80971087-80971106 None:intergenic 40.0%
! AATGGAAAAGTGTGAGGGAA+AGG + chr3.1:80971253-80971272 None:intergenic 40.0%
! ATGGAAAAGTGTGAGGGAAA+GGG + chr3.1:80971252-80971271 None:intergenic 40.0%
! TCCAGCAACATCATTTTCTC+AGG + chr3.1:80972337-80972356 None:intergenic 40.0%
!! AAAGCATTGGCAGTTAGAAG+TGG - chr3.1:80972226-80972245 MS.gene32933:CDS 40.0%
!! CATTGTATTGGTGATGGAAC+TGG - chr3.1:80971551-80971570 MS.gene32933:CDS 40.0%
!! TTGGTGATGGAACTGGTTAT+TGG - chr3.1:80971558-80971577 MS.gene32933:CDS 40.0%
AAGACATGGTGTGTCCATCA+TGG + chr3.1:80971472-80971491 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.1:80971946-80971965 MS.gene32933:CDS 45.0%
ACAATAGGTGGCACATCAAC+AGG + chr3.1:80971416-80971435 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.1:80971282-80971301 None:intergenic 45.0%
ATGATATAGGATGGGCAGCA+TGG - chr3.1:80972032-80972051 MS.gene32933:CDS 45.0%
CGTAAACAACCGAACGAGAA+TGG - chr3.1:80971928-80971947 MS.gene32933:CDS 45.0%
GTCGATTGCAAAAACAGTCC+TGG - chr3.1:80971338-80971357 MS.gene32933:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.1:80971283-80971302 None:intergenic 45.0%
TCATATCCTGGTCAAGAAGG+AGG - chr3.1:80972280-80972299 MS.gene32933:CDS 45.0%
TGAGTGGTGAAACAACAGCA+GGG - chr3.1:80971996-80972015 MS.gene32933:CDS 45.0%
TGCGAAATTCGGTTGAAGAG+TGG - chr3.1:80972095-80972114 MS.gene32933:CDS 45.0%
TGGTTCAAGTCTTCGATTGG+GGG + chr3.1:80971086-80971105 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.1:80971266-80971285 MS.gene32933:CDS 45.0%
!! TGATGATGGGTAGTTCACCA+AGG - chr3.1:80972170-80972189 MS.gene32933:CDS 45.0%
AACAACAGCAGGGGAATTGC+TGG - chr3.1:80972006-80972025 MS.gene32933:CDS 50.0%
AACTCCATCTACGCTGTGAG+TGG - chr3.1:80971980-80971999 MS.gene32933:CDS 50.0%
AAGTCTTCGATTGGGGGACA+TGG + chr3.1:80971080-80971099 None:intergenic 50.0%
CACAGAGCTGTCAACCATGA+TGG - chr3.1:80971455-80971474 MS.gene32933:CDS 50.0%
CATCATGGTTGACAGCTCTG+TGG + chr3.1:80971457-80971476 None:intergenic 50.0%
CGTGCACGTCAGTTACAACA+GGG - chr3.1:80971881-80971900 MS.gene32933:CDS 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.1:80971271-80971290 None:intergenic 50.0%
GAGGTGGTTCCATTCTCGTT+CGG + chr3.1:80971940-80971959 None:intergenic 50.0%
GAGTGGTGAAACAACAGCAG+GGG - chr3.1:80971997-80972016 MS.gene32933:CDS 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.1:80971281-80971300 None:intergenic 50.0%
GTGAGTGGTGAAACAACAGC+AGG - chr3.1:80971995-80972014 MS.gene32933:CDS 50.0%
TATCCTGGTCAAGAAGGAGG+AGG - chr3.1:80972283-80972302 MS.gene32933:CDS 50.0%
TTCACCACTCACAGCGTAGA+TGG + chr3.1:80971987-80972006 None:intergenic 50.0%
CTTCCTCCTCCTTCTTGACC+AGG + chr3.1:80972289-80972308 None:intergenic 55.0%
GCGTGCACGTCAGTTACAAC+AGG - chr3.1:80971880-80971899 MS.gene32933:CDS 55.0%
GGCAGCATGGAAGATTCACC+TGG - chr3.1:80972045-80972064 MS.gene32933:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 80971032 80972408 80971032 ID=MS.gene32933
chr3.1 mRNA 80971032 80972408 80971032 ID=MS.gene32933.t1;Parent=MS.gene32933
chr3.1 exon 80971032 80972408 80971032 ID=MS.gene32933.t1.exon1;Parent=MS.gene32933.t1
chr3.1 CDS 80971032 80972408 80971032 ID=cds.MS.gene32933.t1;Parent=MS.gene32933.t1
Gene Sequence

>MS.gene32933

ATGAACACTTCTTCTCTAAACCTTGTTTCTGAATGTTTTATCAAACCATGTCCCCCAATCGAAGACTTGAACCAAATATGCCATTTATCACATTGGGATATTAGCATGTTATCTGCATACTATATCCAAAAGGGTCTTCTCTTCAAAAAACCAACATCATCATCATCACTAAACCAACAACATTTCATAGAGAACCTAGTAGAGAAACTGAAACACTCCCTTTCCCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTTGTCGATTGCAAAAACAGTCCTGGAGCTAAATTTATTCATGCTACTTTAGATATCACCATCAATGATATTCTCTCACCTGTTGATGTGCCACCTATTGTTCAATCATTCTTTGATCACCACAGAGCTGTCAACCATGATGGACACACCATGTCTTTGTTGTCCATTCAAGTTACTGAATTATTGGATGGTGTTTTTATAGGTTGTTCCATGAATCATTGTATTGGTGATGGAACTGGTTATTGGAATTTCTTTAACTCATTTTCTGAGATATTTCAAAATGATGTTCATGTTCCTGTTCCGATTTCACATAAACCTATTCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATCATCAACCTTCCTTTCAAACATCACGAAGAGTTTATTCATAGATATGAAACACCTATTTTGAGAGAGAGAATATTTCATTTTTCAACAGAGTCTATTGCAAAACTTAAAGCAAAAGCTAACAAAGAGTCTGATATAAACAAAATCTCTTCTTTTCAATCTTTATCAGCACTAGTTTGGAGATCTCTAACGCGTGCACGTCAGTTACAACAGGGTCAACGAACAACTTGCAAATTGGCCGTAAACAACCGAACGAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAACTCCATCTACGCTGTGAGTGGTGAAACAACAGCAGGGGAATTGCTGGAAAATGATATAGGATGGGCAGCATGGAAGATTCACCTGGCTATTGCAAATTATGATGATAAAGTGGTGCGAAATTCGGTTGAAGAGTGGTTACGTTCTCCTTTTGTGTATCGAATGGATCTGTTCTTTGATCCTTATACTGTGATGATGGGTAGTTCACCAAGGTTTAATATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCTGGTCAAGAAGGAGGAGGAAGCATTGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCAGTGTCTGTGTTTAATCCTTTGTTCTAA
Protein sequence

>MS.gene32933.t1

MNTSSLNLVSECFIKPCPPIEDLNQICHLSHWDISMLSAYYIQKGLLFKKPTSSSSLNQQHFIENLVEKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILSPVDVPPIVQSFFDHHRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHCIGDGTGYWNFFNSFSEIFQNDVHVPVPISHKPIHNRWFPEGYGPIINLPFKHHEEFIHRYETPILRERIFHFSTESIAKLKAKANKESDINKISSFQSLSALVWRSLTRARQLQQGQRTTCKLAVNNRTRMEPPLPKEYFGNSIYAVSGETTAGELLENDIGWAAWKIHLAIANYDDKVVRNSVEEWLRSPFVYRMDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGQEGGGSIDLEVCLSPEKMMLLETDEEFMNSVSVFNPLF