AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012961

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012961.t1 MTR_3g101930 75.810 463 100 5 1 461 2 454 0.0 730
MS.gene012961.t1 MTR_3g101900 74.298 463 104 5 1 463 2 449 0.0 703
MS.gene012961.t1 MTR_3g102020 67.177 457 139 5 9 465 9 454 0.0 632
MS.gene012961.t1 MTR_3g101960 67.929 449 132 6 9 457 9 445 0.0 627
MS.gene012961.t1 MTR_3g102010 65.385 468 144 7 1 465 2 454 0.0 615
MS.gene012961.t1 MTR_3g101970 64.615 455 136 5 9 463 9 438 0.0 613
MS.gene012961.t1 MTR_3g102030 56.707 328 106 6 88 415 29 320 3.02e-129 377
MS.gene012961.t1 MTR_4g015180 37.016 516 246 10 1 464 1 489 2.71e-91 286
MS.gene012961.t1 MTR_4g094842 39.479 461 247 14 9 459 7 445 2.37e-90 282
MS.gene012961.t1 MTR_3g070170 36.049 491 246 10 3 458 9 466 1.34e-88 278
MS.gene012961.t1 MTR_5g081960 35.547 512 246 12 1 458 1 482 5.06e-87 275
MS.gene012961.t1 MTR_3g088420 37.978 445 241 9 24 462 18 433 2.13e-85 269
MS.gene012961.t1 MTR_4g022800 37.225 454 245 12 22 462 16 442 7.22e-85 268
MS.gene012961.t1 MTR_0190s0020 36.726 452 253 10 18 462 17 442 8.85e-84 265
MS.gene012961.t1 MTR_8g041770 36.322 435 252 8 30 462 30 441 5.65e-82 260
MS.gene012961.t1 MTR_4g022670 37.528 453 244 12 22 462 16 441 1.36e-81 259
MS.gene012961.t1 MTR_4g022720 38.031 447 244 10 22 462 16 435 6.56e-81 258
MS.gene012961.t1 MTR_4g094835 36.591 440 247 10 26 458 28 442 2.04e-79 254
MS.gene012961.t1 MTR_4g094838 35.011 437 240 10 30 458 28 428 1.44e-77 249
MS.gene012961.t1 MTR_4g022825 36.629 445 242 11 30 462 24 440 3.24e-76 245
MS.gene012961.t1 MTR_3g026310 36.526 449 245 10 30 462 24 448 2.00e-75 244
MS.gene012961.t1 MTR_4g094828 35.920 451 250 12 27 463 26 451 4.40e-75 243
MS.gene012961.t1 MTR_7g026860 35.683 454 248 13 22 462 16 438 1.14e-74 241
MS.gene012961.t1 MTR_4g022290 32.962 449 260 10 24 460 24 443 6.70e-69 226
MS.gene012961.t1 MTR_4g094832 35.539 408 233 10 63 458 37 426 9.56e-68 223
MS.gene012961.t1 MTR_8g079710 34.615 442 249 14 22 451 21 434 2.77e-65 217
MS.gene012961.t1 MTR_4g022760 36.785 367 196 9 22 376 16 358 1.95e-61 206
MS.gene012961.t1 MTR_6g045553 29.268 492 273 10 10 461 18 474 1.41e-53 187
MS.gene012961.t1 MTR_6g016815 30.435 437 207 14 30 462 38 381 5.05e-40 148
MS.gene012961.t1 MTR_8g040780 36.842 209 128 3 252 457 20 227 9.70e-38 138
MS.gene012961.t1 MTR_4g007540 29.056 413 230 16 56 443 44 418 6.51e-37 140
MS.gene012961.t1 MTR_2g064495 28.605 423 236 15 56 452 44 426 2.77e-36 139
MS.gene012961.t1 MTR_2g105330 28.369 423 237 15 56 452 44 426 7.67e-36 138
MS.gene012961.t1 MTR_8g075610 27.865 384 242 12 70 443 47 405 8.55e-35 134
MS.gene012961.t1 MTR_3g098980 31.818 308 181 11 70 369 66 352 8.96e-34 132
MS.gene012961.t1 MTR_8g096530 29.936 314 192 9 64 369 53 346 1.36e-32 129
MS.gene012961.t1 MTR_3g049150 26.420 405 250 11 57 437 45 425 5.80e-31 124
MS.gene012961.t1 MTR_7g026770 38.547 179 87 2 165 343 132 287 4.93e-28 113
MS.gene012961.t1 MTR_3g026950 25.545 321 201 8 67 369 51 351 4.45e-27 113
MS.gene012961.t1 MTR_7g026850 40.625 160 71 5 44 200 21 159 5.37e-27 107
MS.gene012961.t1 MTR_4g127650 25.577 477 283 19 9 452 12 449 2.83e-26 111
MS.gene012961.t1 MTR_4g097090 23.913 460 286 14 13 443 16 440 5.41e-23 101
MS.gene012961.t1 MTR_4g094825 35.333 150 86 2 313 462 253 391 7.37e-23 100
MS.gene012961.t1 MTR_4g094825 33.526 173 101 4 30 202 92 250 4.60e-19 89.4
MS.gene012961.t1 MTR_7g027175 25.543 368 239 12 57 416 55 395 8.48e-23 100
MS.gene012961.t1 MTR_3g087640 27.570 428 246 15 56 443 53 456 9.79e-23 100
MS.gene012961.t1 MTR_6g053810 25.815 368 244 11 13 373 18 363 1.65e-22 100
MS.gene012961.t1 MTR_7g114520 26.108 406 255 17 70 458 66 443 3.04e-22 99.4
MS.gene012961.t1 MTR_7g114550 25.581 430 279 16 44 458 37 440 4.65e-22 99.0
MS.gene012961.t1 MTR_1g040195 25.872 344 225 11 92 430 58 376 2.25e-21 96.7
MS.gene012961.t1 MTR_8g040760 33.178 214 122 5 22 234 19 212 5.75e-21 91.7
MS.gene012961.t1 MTR_1g040175 26.629 353 219 15 70 411 64 387 7.24e-21 95.5
MS.gene012961.t1 MTR_5g076700 24.074 432 284 17 44 458 37 441 7.40e-21 95.1
MS.gene012961.t1 MTR_7g085990 25.339 442 252 18 56 452 42 450 2.65e-20 93.6
MS.gene012961.t1 MTR_1g053315 24.283 453 287 20 14 443 26 445 3.16e-20 93.6
MS.gene012961.t1 MTR_7g114490 26.941 438 253 20 44 453 37 435 9.80e-20 92.0
MS.gene012961.t1 MTR_7g086000 27.494 411 244 17 73 452 63 450 1.51e-19 91.3
MS.gene012961.t1 MTR_3g071320 27.586 319 202 8 69 375 59 360 1.85e-19 90.9
MS.gene012961.t1 MTR_7g114960 25.339 442 290 14 30 458 30 444 2.16e-19 90.9
MS.gene012961.t1 MTR_4g013050 25.939 293 167 8 68 357 51 296 6.43e-19 89.0
MS.gene012961.t1 MTR_3g465980 26.518 313 189 12 48 346 49 334 9.22e-19 89.0
MS.gene012961.t1 MTR_3g465990 27.063 303 183 11 58 346 56 334 1.17e-18 88.6
MS.gene012961.t1 MTR_1g081660 26.891 357 219 16 67 411 70 396 1.39e-18 88.2
MS.gene012961.t1 MTR_1g040215 24.091 440 290 17 1 431 1 405 2.67e-18 87.4
MS.gene012961.t1 MTR_7g085930 25.175 429 261 18 56 448 46 450 2.89e-18 87.4
MS.gene012961.t1 MTR_4g055170 26.736 288 180 9 70 346 69 336 2.98e-18 87.4
MS.gene012961.t1 MTR_6g034495 39.844 128 65 5 81 205 45 163 5.21e-18 81.6
MS.gene012961.t1 MTR_6g034485 34.677 124 81 0 216 339 2 125 1.11e-17 79.3
MS.gene012961.t1 MTR_7g114470 23.636 440 277 18 44 458 38 443 1.24e-17 85.5
MS.gene012961.t1 MTR_3g450710 23.238 383 229 14 64 411 59 411 4.30e-17 84.0
MS.gene012961.t1 MTR_3g112180 24.658 365 223 10 67 411 58 390 8.68e-17 82.8
MS.gene012961.t1 MTR_7g084940 25.466 322 207 11 64 374 63 362 1.30e-16 82.4
MS.gene012961.t1 MTR_2g089765 23.605 466 278 17 24 443 33 466 1.06e-15 79.7
MS.gene012961.t1 MTR_0502s0010 28.514 249 145 10 114 349 2 230 2.12e-15 75.9
MS.gene012961.t1 MTR_7g014330 25.547 411 255 16 30 413 26 412 4.23e-15 77.8
MS.gene012961.t1 MTR_1g040315 25.212 353 208 14 1 337 1 313 5.48e-15 77.0
MS.gene012961.t1 MTR_3g049140 26.214 206 139 4 245 437 18 223 7.47e-15 74.7
MS.gene012961.t1 MTR_3g028210 23.544 412 273 13 56 441 53 448 1.25e-14 76.3
MS.gene012961.t1 MTR_5g090810 23.421 380 220 16 70 411 66 412 1.88e-14 75.9
MS.gene012961.t1 MTR_6g015815 23.701 481 301 18 6 453 7 454 2.38e-14 75.5
MS.gene012961.t1 MTR_7g013850 23.261 460 279 18 31 441 30 464 2.59e-14 75.5
MS.gene012961.t1 MTR_3g465930 24.912 285 190 6 70 346 69 337 4.80e-14 74.3
MS.gene012961.t1 MTR_6g015850 24.419 430 257 15 57 453 57 451 6.12e-14 73.9
MS.gene012961.t1 MTR_3g450690 22.222 378 234 15 65 411 67 415 9.03e-14 73.6
MS.gene012961.t1 MTR_8g012750 24.368 435 257 19 19 409 15 421 9.61e-14 73.6
MS.gene012961.t1 MTR_7g026830 55.556 54 24 0 136 189 44 97 1.88e-13 66.6
MS.gene012961.t1 MTR_2g015430 23.077 312 198 10 73 369 61 345 2.48e-13 72.0
MS.gene012961.t1 MTR_2g089670 23.155 393 231 14 56 409 68 428 4.54e-13 71.6
MS.gene012961.t1 MTR_3g465910 25.000 292 177 10 71 346 69 334 4.69e-13 71.6
MS.gene012961.t1 MTR_3g465880 26.736 288 182 10 71 346 69 339 6.01e-13 71.2
MS.gene012961.t1 MTR_7g056087 23.145 337 220 13 56 374 45 360 1.61e-12 69.7
MS.gene012961.t1 MTR_7g084910 25.362 276 173 10 64 327 64 318 6.68e-12 67.8
MS.gene012961.t1 MTR_2g045540 32.990 97 57 1 245 341 18 106 9.61e-12 62.4
MS.gene012961.t1 MTR_6g015315 24.641 418 243 17 62 443 55 436 1.19e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012961.t1 AT5G42830 53.642 453 192 6 13 463 14 450 1.86e-168 482
MS.gene012961.t1 AT5G07850 52.165 462 208 8 2 462 4 453 1.86e-163 469
MS.gene012961.t1 AT5G07870 48.408 471 220 7 2 462 4 461 2.04e-143 419
MS.gene012961.t1 AT5G07860 49.119 454 214 7 9 461 11 448 1.99e-142 416
MS.gene012961.t1 AT2G39980 37.657 478 219 12 34 462 33 480 1.27e-92 289
MS.gene012961.t1 AT5G67150 36.087 460 271 8 9 464 8 448 5.87e-89 279
MS.gene012961.t1 AT5G01210 36.454 502 254 10 1 466 1 473 1.03e-85 271
MS.gene012961.t1 AT3G50270 33.120 468 273 13 9 463 9 449 7.34e-76 245
MS.gene012961.t1 AT3G50280 32.675 456 278 10 9 458 7 439 5.28e-75 242
MS.gene012961.t1 AT5G67160 33.045 463 267 13 9 464 8 434 3.92e-70 229
MS.gene012961.t1 AT3G50300 33.806 423 248 12 25 439 22 420 4.03e-68 224
MS.gene012961.t1 AT5G38130 33.710 442 260 15 9 443 27 442 1.03e-67 224
MS.gene012961.t1 AT3G50280 36.364 308 185 5 156 458 8 309 5.46e-59 196
MS.gene012961.t1 AT5G23940 30.369 461 292 9 9 458 6 448 1.30e-55 192
MS.gene012961.t1 AT3G50290 39.464 261 143 6 157 408 15 269 2.51e-52 178
MS.gene012961.t1 AT5G48930 29.250 400 242 13 68 452 54 427 4.66e-37 141
MS.gene012961.t1 AT3G48720 31.897 348 202 12 17 357 17 336 2.53e-35 136
MS.gene012961.t1 AT5G41040 28.609 381 223 17 64 428 63 410 2.72e-34 134
MS.gene012961.t1 AT5G41040 28.609 381 223 17 64 428 79 426 2.98e-34 134
MS.gene012961.t1 AT5G63560 28.431 408 238 16 17 412 17 382 2.06e-32 128
MS.gene012961.t1 AT2G19070 28.097 452 264 20 30 452 25 444 8.66e-32 127
MS.gene012961.t1 AT5G57840 27.021 470 270 21 8 449 4 428 1.93e-22 100
MS.gene012961.t1 AT1G03940 24.885 434 261 21 57 453 53 458 2.71e-21 96.7
MS.gene012961.t1 AT2G40230 26.020 392 251 12 40 421 35 397 3.50e-21 96.3
MS.gene012961.t1 AT1G28680 24.919 309 201 12 73 374 65 349 2.84e-20 92.8
MS.gene012961.t1 AT1G28680 24.919 309 201 12 73 374 65 349 3.16e-20 93.6
MS.gene012961.t1 AT1G78990 25.474 369 213 13 68 411 65 396 8.56e-20 92.0
MS.gene012961.t1 AT1G03495 28.481 316 169 18 57 338 53 345 9.37e-20 90.9
MS.gene012961.t1 AT1G65450 28.041 296 176 11 68 347 70 344 6.39e-19 89.4
MS.gene012961.t1 AT1G31490 26.353 425 250 18 56 457 48 432 1.65e-18 88.2
MS.gene012961.t1 AT3G26040 24.396 414 257 13 67 463 56 430 4.42e-18 86.7
MS.gene012961.t1 AT1G03390 24.697 413 264 15 55 448 64 448 5.69e-17 83.6
MS.gene012961.t1 AT3G29635 25.542 415 236 20 30 409 29 405 2.31e-16 81.6
MS.gene012961.t1 AT1G27620 24.931 361 218 12 70 411 58 384 3.06e-16 81.3
MS.gene012961.t1 AT5G17540 25.545 321 199 12 44 349 36 331 3.05e-15 78.2
MS.gene012961.t1 AT5G07080 23.841 302 190 11 55 341 55 331 6.39e-15 77.0
MS.gene012961.t1 AT2G25150 25.714 315 199 11 70 371 67 359 5.78e-14 74.3
MS.gene012961.t1 AT1G65450 28.877 187 111 6 176 347 1 180 2.79e-13 70.9
MS.gene012961.t1 AT5G39090 22.973 444 288 17 30 451 28 439 5.93e-13 71.2
MS.gene012961.t1 AT3G29590 24.422 389 248 15 77 443 68 432 2.56e-12 68.9
MS.gene012961.t1 AT5G39080 21.199 467 282 15 30 451 28 453 1.26e-11 67.0

Find 100 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTGATCTATTGTTTA+TGG 0.126098 3.4:+89759375 None:intergenic
AAATAATTTCTCTCTCAATT+TGG 0.143020 3.4:+89759558 None:intergenic
ATTTGTATTGGATTCTTTAT+TGG 0.177909 3.4:+89759495 None:intergenic
TGCTTTGAATCTTCAATAAT+AGG 0.188678 3.4:+89760246 None:intergenic
ATGAAATCTTGTTGGTTATT+TGG 0.209059 3.4:+89760135 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.211414 3.4:-89759106 MS.gene012961:CDS
GAATTGCTTGAGAATGATTT+AGG 0.262759 3.4:-89759283 MS.gene012961:CDS
AAGCAAATTTGTTACTTAAC+AGG 0.274512 3.4:-89760228 MS.gene012961:CDS
ATCTTCCTTCCAATCTTTAT+CGG 0.278893 3.4:-89759467 MS.gene012961:CDS
AATAATTTCTCTCTCAATTT+GGG 0.299647 3.4:+89759559 None:intergenic
GCTTTGAATCTTCAATAATA+GGG 0.313536 3.4:+89760247 None:intergenic
TTAGTGGACCGTAACCTTCT+AGG 0.322324 3.4:+89759635 None:intergenic
ATCAACAAACATGGTATAAG+AGG 0.329053 3.4:+89760011 None:intergenic
AATCTTTATCGGCATTTGTT+TGG 0.337842 3.4:-89759456 MS.gene012961:CDS
AGATTTCATTCAGAATCTAT+TGG 0.344488 3.4:-89760121 MS.gene012961:CDS
CATAATCGGTGGTTCCTAGA+AGG 0.366417 3.4:-89759649 MS.gene012961:CDS
TCACCAAGGTTCAATATGTA+TGG 0.368741 3.4:-89759118 MS.gene012961:CDS
GATTCTGAATGAAATCTTGT+TGG 0.378418 3.4:+89760127 None:intergenic
AGGAGGAGGAAGCATGGATT+TGG 0.380210 3.4:-89759005 MS.gene012961:CDS
CATGCCGTTGCTATCCATTC+AGG 0.380410 3.4:-89759824 MS.gene012961:CDS
AATGAGAACATCATCGCCTT+TGG 0.401999 3.4:+89759687 None:intergenic
AAACAAATGCCGATAAAGAT+TGG 0.404666 3.4:+89759458 None:intergenic
CACTCATTGCATTAGGCAAA+AGG 0.405527 3.4:+89758975 None:intergenic
TCAATGCACTACATCCAAAA+AGG 0.406674 3.4:-89760186 MS.gene012961:CDS
CCATGCTGAAGGGATCAAAA+TGG 0.415288 3.4:+89759152 None:intergenic
GTTTATGGCTAACTTGCAAC+TGG 0.422888 3.4:+89759390 None:intergenic
TGAAATTCTTTGAGTGATAG+AGG 0.426454 3.4:+89760293 None:intergenic
GGATATGCAAACAAATTTGA+TGG 0.429813 3.4:-89759055 MS.gene012961:CDS
TGGTTATTTGGTGAAGTTGT+TGG 0.431090 3.4:+89760147 None:intergenic
ATTTGAAGGAGGAGGAAGCA+TGG 0.434087 3.4:-89759011 MS.gene012961:CDS
AGTTTCCAAAGTACTCTTGC+GGG 0.444358 3.4:+89759341 None:intergenic
CCAAAGTACTCTTGCGGGAG+AGG 0.455688 3.4:+89759346 None:intergenic
TGCTTGAGAATGATTTAGGT+TGG 0.465724 3.4:-89759279 MS.gene012961:CDS
CCTCTCCCGCAAGAGTACTT+TGG 0.478058 3.4:-89759346 MS.gene012961:CDS
GCTTGAGAATGATTTAGGTT+GGG 0.480817 3.4:-89759278 MS.gene012961:CDS
ACGATTGGCGGAGCATCAAC+CGG 0.481258 3.4:+89759895 None:intergenic
TCTAGCGCACTCATTGCATT+AGG 0.481834 3.4:+89758968 None:intergenic
AAATTTGTTACTTAACAGGC+TGG 0.483511 3.4:-89760224 MS.gene012961:CDS
TCCTCCTTCAAATCCCTCAT+AGG 0.483907 3.4:+89759021 None:intergenic
TATCGATAAAGCAATCAATC+AGG 0.488699 3.4:-89759926 MS.gene012961:CDS
CGACCAAGTTGGTAGACAAC+CGG 0.489315 3.4:+89759175 None:intergenic
TTGAACGGAAGATTAATTAG+TGG 0.490884 3.4:+89759619 None:intergenic
TAGATTCATCTATGCAACTT+TGG 0.497446 3.4:-89759965 MS.gene012961:CDS
AGGAACCACCGATTATGAAA+AGG 0.505726 3.4:+89759655 None:intergenic
TTCCCATACATATTGAACCT+TGG 0.519386 3.4:+89759115 None:intergenic
CGGTGGTTCCTAGAAGGTTA+CGG 0.519415 3.4:-89759643 MS.gene012961:CDS
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.4:-89759100 MS.gene012961:CDS
CACTAGCTCAGTGACCTGAA+TGG 0.523158 3.4:+89759810 None:intergenic
ACAGCCTATGAGGGATTTGA+AGG 0.524136 3.4:-89759025 MS.gene012961:CDS
TCAAACAACGAGTCCACGAT+TGG 0.525042 3.4:+89759880 None:intergenic
TGGCAATGTGACAGCCTATG+AGG 0.538642 3.4:-89759035 MS.gene012961:CDS
TGCATAGATGAATCTAGCTC+CGG 0.547120 3.4:+89759972 None:intergenic
TTTAGGTTGGGCAGCGTGGA+AGG 0.547420 3.4:-89759266 MS.gene012961:CDS
ACTTGACATCACCATGCTGA+AGG 0.551570 3.4:+89759141 None:intergenic
AAATTGCATTCATAAAATCC+TGG 0.553575 3.4:+89758939 None:intergenic
CATTCAGGTCACTGAGCTAG+TGG 0.558225 3.4:-89759809 MS.gene012961:CDS
AATCAATCAGGATGCTCCAC+CGG 0.558802 3.4:-89759914 MS.gene012961:CDS
ATGGAAAAGAGCATTAGAGA+GGG 0.559413 3.4:+89760083 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.4:-89759117 MS.gene012961:CDS
AATGGAAAAGAGCATTAGAG+AGG 0.561847 3.4:+89760082 None:intergenic
AACTCGTCAAAATGTTTGAA+CGG 0.562459 3.4:+89759604 None:intergenic
GGTTGATGCTCCGCCAATCG+TGG 0.563064 3.4:-89759893 MS.gene012961:CDS
TATGAGGGATTTGAAGGAGG+AGG 0.567796 3.4:-89759019 MS.gene012961:CDS
AAATGCCGATAAAGATTGGA+AGG 0.570447 3.4:+89759462 None:intergenic
ACTCATTGCATTAGGCAAAA+GGG 0.570495 3.4:+89758976 None:intergenic
ATGAACCATTGTGTTGGTGA+TGG 0.571518 3.4:-89759763 MS.gene012961:CDS
GATCAAAATGGCGACCAAGT+TGG 0.573234 3.4:+89759164 None:intergenic
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.4:-89759101 MS.gene012961:CDS
TCTCCGGTTGTCTACCAACT+TGG 0.573892 3.4:-89759178 MS.gene012961:CDS
TGAACCATTGTGTTGGTGAT+GGG 0.579011 3.4:-89759762 MS.gene012961:CDS
GATAGAGCAATCAATCATGA+TGG 0.585089 3.4:-89759853 MS.gene012961:CDS
AAGTACTCTTGCGGGAGAGG+TGG 0.588714 3.4:+89759349 None:intergenic
TCTTCAATAATAGGGTGTAG+TGG 0.589086 3.4:+89760255 None:intergenic
CAGGTCACTGAGCTAGTGGA+TGG 0.594154 3.4:-89759805 MS.gene012961:CDS
AATTTGTTACTTAACAGGCT+GGG 0.594863 3.4:-89760223 MS.gene012961:CDS
AACAACGAGTCCACGATTGG+CGG 0.603442 3.4:+89759883 None:intergenic
TGAATTTGGAATGGGAAAAG+CGG 0.603934 3.4:-89759092 MS.gene012961:CDS
AAAGCGGTTGCAGTTCTAAG+TGG 0.606695 3.4:-89759076 MS.gene012961:CDS
CTTTGGAAACTCAGTTGACG+TGG 0.608160 3.4:-89759329 MS.gene012961:CDS
ATGATTTAGGTTGGGCAGCG+TGG 0.611214 3.4:-89759270 MS.gene012961:CDS
ATATATCAAGCTCAAGCCAA+AGG 0.614342 3.4:-89759703 MS.gene012961:CDS
TCAACAAACATGGTATAAGA+GGG 0.616324 3.4:+89760012 None:intergenic
GAGTTTCCAAAGTACTCTTG+CGG 0.617099 3.4:+89759340 None:intergenic
ACAAACATGGTATAAGAGGG+AGG 0.618261 3.4:+89760015 None:intergenic
CTTGACATCACCATGCTGAA+GGG 0.623581 3.4:+89759142 None:intergenic
GTGACCTGAATGGATAGCAA+CGG 0.634165 3.4:+89759820 None:intergenic
GCCTATGAGGGATTTGAAGG+AGG 0.640344 3.4:-89759022 MS.gene012961:CDS
GGCAATGTGACAGCCTATGA+GGG 0.641579 3.4:-89759034 MS.gene012961:CDS
TATTGCAAAACTGAAAGCAA+AGG 0.644608 3.4:-89759518 MS.gene012961:CDS
TGTGAATCACGACGATAGAG+AGG 0.645383 3.4:-89759230 MS.gene012961:CDS
ACCACCGATTATGAAAAGGT+TGG 0.648796 3.4:+89759659 None:intergenic
GAGTGCGCTAGAATCAGACC+AGG 0.652006 3.4:-89758957 MS.gene012961:CDS
TGTTCAATGAACCATTGTGT+TGG 0.653933 3.4:-89759769 MS.gene012961:CDS
CAAACATGGTATAAGAGGGA+GGG 0.664234 3.4:+89760016 None:intergenic
AAGTCCCATCACCAACACAA+TGG 0.687793 3.4:+89759758 None:intergenic
ATTGGCGGAGCATCAACCGG+TGG 0.689437 3.4:+89759898 None:intergenic
GTTACTGCAATCAACAAACA+TGG 0.692327 3.4:+89760002 None:intergenic
CTGAATGGATAGCAACGGCA+TGG 0.698801 3.4:+89759825 None:intergenic
GATTGCAGTAACGATAACAC+CGG 0.716514 3.4:-89759991 MS.gene012961:CDS
TGGTGATGTCAAGTTCACCA+AGG 0.737551 3.4:-89759132 MS.gene012961:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGAATTTCTTCAATATA+TGG - chr3.4:89759478-89759497 MS.gene012961:CDS 15.0%
!! AAATAATTTCTCTCTCAATT+TGG + chr3.4:89759655-89759674 None:intergenic 20.0%
!! AATAATTTCTCTCTCAATTT+GGG + chr3.4:89759654-89759673 None:intergenic 20.0%
!! ATTTGTATTGGATTCTTTAT+TGG + chr3.4:89759718-89759737 None:intergenic 20.0%
!!! GGAAGATATTTTATTTGTAT+TGG + chr3.4:89759730-89759749 None:intergenic 20.0%
!!! TTGTTGGTTTTTTGAATAAT+AGG + chr3.4:89759050-89759069 None:intergenic 20.0%
! AAATTGCATTCATAAAATCC+TGG + chr3.4:89760274-89760293 None:intergenic 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.4:89760104-89760123 MS.gene012961:CDS 25.0%
! AGATTTCATTCAGAATCTAT+TGG - chr3.4:89759089-89759108 MS.gene012961:CDS 25.0%
! GATTCTTGATCTATTGTTTA+TGG + chr3.4:89759838-89759857 None:intergenic 25.0%
!! ATGAAATCTTGTTGGTTATT+TGG + chr3.4:89759078-89759097 None:intergenic 25.0%
!! GCTTTGAATCTTCAATAATA+GGG + chr3.4:89758966-89758985 None:intergenic 25.0%
!! TGCTTTGAATCTTCAATAAT+AGG + chr3.4:89758967-89758986 None:intergenic 25.0%
!! TTTTATGAATGCAATTTCTG+TGG - chr3.4:89760277-89760296 MS.gene012961:CDS 25.0%
!! TTTTGAATAATAGGCCTTTT+TGG + chr3.4:89759041-89759060 None:intergenic 25.0%
!!! AAGCAAATTTGTTACTTAAC+AGG - chr3.4:89758982-89759001 MS.gene012961:CDS 25.0%
AAACAAATGCCGATAAAGAT+TGG + chr3.4:89759755-89759774 None:intergenic 30.0%
AACTCGTCAAAATGTTTGAA+CGG + chr3.4:89759609-89759628 None:intergenic 30.0%
AATCTTTATCGGCATTTGTT+TGG - chr3.4:89759754-89759773 MS.gene012961:CDS 30.0%
ATCAACAAACATGGTATAAG+AGG + chr3.4:89759202-89759221 None:intergenic 30.0%
ATCTTCCTTCCAATCTTTAT+CGG - chr3.4:89759743-89759762 MS.gene012961:CDS 30.0%
GAATTGCTTGAGAATGATTT+AGG - chr3.4:89759927-89759946 MS.gene012961:CDS 30.0%
GATTCTGAATGAAATCTTGT+TGG + chr3.4:89759086-89759105 None:intergenic 30.0%
GGATATGCAAACAAATTTGA+TGG - chr3.4:89760155-89760174 MS.gene012961:CDS 30.0%
TAGATTCATCTATGCAACTT+TGG - chr3.4:89759245-89759264 MS.gene012961:CDS 30.0%
TATCGATAAAGCAATCAATC+AGG - chr3.4:89759284-89759303 MS.gene012961:CDS 30.0%
TATTGCAAAACTGAAAGCAA+AGG - chr3.4:89759692-89759711 MS.gene012961:CDS 30.0%
TCAACAAACATGGTATAAGA+GGG + chr3.4:89759201-89759220 None:intergenic 30.0%
TGAAATTCTTTGAGTGATAG+AGG + chr3.4:89758920-89758939 None:intergenic 30.0%
TTGAACGGAAGATTAATTAG+TGG + chr3.4:89759594-89759613 None:intergenic 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.4:89760110-89760129 MS.gene012961:CDS 30.0%
! TTCCATTTCTATCCTCTTTT+TGG - chr3.4:89759144-89759163 MS.gene012961:CDS 30.0%
!! AAATTTGTTACTTAACAGGC+TGG - chr3.4:89758986-89759005 MS.gene012961:CDS 30.0%
!! AATTTGTTACTTAACAGGCT+GGG - chr3.4:89758987-89759006 MS.gene012961:CDS 30.0%
AAATGCCGATAAAGATTGGA+AGG + chr3.4:89759751-89759770 None:intergenic 35.0%
ACTCATTGCATTAGGCAAAA+GGG + chr3.4:89760237-89760256 None:intergenic 35.0%
ATATATCAAGCTCAAGCCAA+AGG - chr3.4:89759507-89759526 MS.gene012961:CDS 35.0%
CAAAAATTGGTTAGAGTCTC+CGG - chr3.4:89760016-89760035 MS.gene012961:CDS 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.4:89760093-89760112 MS.gene012961:CDS 35.0%
GATAGAGCAATCAATCATGA+TGG - chr3.4:89759357-89759376 MS.gene012961:CDS 35.0%
GTAAGTACAAAAACGACTGT+AGG - chr3.4:89759903-89759922 MS.gene012961:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.4:89760109-89760128 MS.gene012961:CDS 35.0%
GTTACTGCAATCAACAAACA+TGG + chr3.4:89759211-89759230 None:intergenic 35.0%
TCAATGCACTACATCCAAAA+AGG - chr3.4:89759024-89759043 MS.gene012961:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.4:89760092-89760111 MS.gene012961:CDS 35.0%
TCTTCAATAATAGGGTGTAG+TGG + chr3.4:89758958-89758977 None:intergenic 35.0%
TGAGAGAGATGCTCAAAAAT+TGG - chr3.4:89760003-89760022 MS.gene012961:CDS 35.0%
TGCTTGAGAATGATTTAGGT+TGG - chr3.4:89759931-89759950 MS.gene012961:CDS 35.0%
TGTTCAATGAACCATTGTGT+TGG - chr3.4:89759441-89759460 MS.gene012961:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.4:89760098-89760117 None:intergenic 35.0%
! GCTTGAGAATGATTTAGGTT+GGG - chr3.4:89759932-89759951 MS.gene012961:CDS 35.0%
! TGAATTTGGAATGGGAAAAG+CGG - chr3.4:89760118-89760137 MS.gene012961:CDS 35.0%
! TGGTTATTTGGTGAAGTTGT+TGG + chr3.4:89759066-89759085 None:intergenic 35.0%
!! AATGGAAAAGAGCATTAGAG+AGG + chr3.4:89759131-89759150 None:intergenic 35.0%
!! ATGGAAAAGAGCATTAGAGA+GGG + chr3.4:89759130-89759149 None:intergenic 35.0%
AATGAGAACATCATCGCCTT+TGG + chr3.4:89759526-89759545 None:intergenic 40.0%
ACAAACATGGTATAAGAGGG+AGG + chr3.4:89759198-89759217 None:intergenic 40.0%
CAAACATGGTATAAGAGGGA+GGG + chr3.4:89759197-89759216 None:intergenic 40.0%
CACTCATTGCATTAGGCAAA+AGG + chr3.4:89760238-89760257 None:intergenic 40.0%
GATTGCAGTAACGATAACAC+CGG - chr3.4:89759219-89759238 MS.gene012961:CDS 40.0%
GGCCAAAAAGAGGATAGAAA+TGG + chr3.4:89759149-89759168 None:intergenic 40.0%
! ACCAACCTTTTCATAATCGG+TGG - chr3.4:89759550-89759569 MS.gene012961:CDS 40.0%
! ACCACCGATTATGAAAAGGT+TGG + chr3.4:89759554-89759573 None:intergenic 40.0%
! AGGAACCACCGATTATGAAA+AGG + chr3.4:89759558-89759577 None:intergenic 40.0%
! AGTTTCCAAAGTACTCTTGC+GGG + chr3.4:89759872-89759891 None:intergenic 40.0%
! ATGAACCATTGTGTTGGTGA+TGG - chr3.4:89759447-89759466 MS.gene012961:CDS 40.0%
! CGCACCAACCTTTTCATAAT+CGG - chr3.4:89759547-89759566 MS.gene012961:CDS 40.0%
! GAGTTTCCAAAGTACTCTTG+CGG + chr3.4:89759873-89759892 None:intergenic 40.0%
! GTTTATGGCTAACTTGCAAC+TGG + chr3.4:89759823-89759842 None:intergenic 40.0%
! TGCATAGATGAATCTAGCTC+CGG + chr3.4:89759241-89759260 None:intergenic 40.0%
!! CTAGTGGATGGTGTTTTCAT+AGG - chr3.4:89759417-89759436 MS.gene012961:CDS 40.0%
!! GAGTTTTGTGAGTGACAAGA+CGG + chr3.4:89759170-89759189 None:intergenic 40.0%
!! TGAACCATTGTGTTGGTGAT+GGG - chr3.4:89759448-89759467 MS.gene012961:CDS 40.0%
AAGTCCCATCACCAACACAA+TGG + chr3.4:89759455-89759474 None:intergenic 45.0%
AATCAATCAGGATGCTCCAC+CGG - chr3.4:89759296-89759315 MS.gene012961:CDS 45.0%
ACAGCCTATGAGGGATTTGA+AGG - chr3.4:89760185-89760204 MS.gene012961:CDS 45.0%
ACTTGACATCACCATGCTGA+AGG + chr3.4:89760072-89760091 None:intergenic 45.0%
ATTTGAAGGAGGAGGAAGCA+TGG - chr3.4:89760199-89760218 MS.gene012961:CDS 45.0%
CATAATCGGTGGTTCCTAGA+AGG - chr3.4:89759561-89759580 MS.gene012961:CDS 45.0%
CCATGCTGAAGGGATCAAAA+TGG + chr3.4:89760061-89760080 None:intergenic 45.0%
CTTGACATCACCATGCTGAA+GGG + chr3.4:89760071-89760090 None:intergenic 45.0%
GATCAAAATGGCGACCAAGT+TGG + chr3.4:89760049-89760068 None:intergenic 45.0%
GTGACCTGAATGGATAGCAA+CGG + chr3.4:89759393-89759412 None:intergenic 45.0%
TATGAGGGATTTGAAGGAGG+AGG - chr3.4:89760191-89760210 MS.gene012961:CDS 45.0%
TCAAACAACGAGTCCACGAT+TGG + chr3.4:89759333-89759352 None:intergenic 45.0%
TCCTCCTTCAAATCCCTCAT+AGG + chr3.4:89760192-89760211 None:intergenic 45.0%
TCTAGCGCACTCATTGCATT+AGG + chr3.4:89760245-89760264 None:intergenic 45.0%
TGTGAATCACGACGATAGAG+AGG - chr3.4:89759980-89759999 MS.gene012961:CDS 45.0%
! CTTTGGAAACTCAGTTGACG+TGG - chr3.4:89759881-89759900 MS.gene012961:CDS 45.0%
! TGGTGATGTCAAGTTCACCA+AGG - chr3.4:89760078-89760097 MS.gene012961:CDS 45.0%
! TTAGTGGACCGTAACCTTCT+AGG + chr3.4:89759578-89759597 None:intergenic 45.0%
!! AAAGCGGTTGCAGTTCTAAG+TGG - chr3.4:89760134-89760153 MS.gene012961:CDS 45.0%
!! CCATTTTGATCCCTTCAGCA+TGG - chr3.4:89760058-89760077 MS.gene012961:CDS 45.0%
AACAACGAGTCCACGATTGG+CGG + chr3.4:89759330-89759349 None:intergenic 50.0%
CACTAGCTCAGTGACCTGAA+TGG + chr3.4:89759403-89759422 None:intergenic 50.0%
CATGCCGTTGCTATCCATTC+AGG - chr3.4:89759386-89759405 MS.gene012961:CDS 50.0%
CATTCAGGTCACTGAGCTAG+TGG - chr3.4:89759401-89759420 MS.gene012961:CDS 50.0%
CGGTGGTTCCTAGAAGGTTA+CGG - chr3.4:89759567-89759586 MS.gene012961:CDS 50.0%
CTGAATGGATAGCAACGGCA+TGG + chr3.4:89759388-89759407 None:intergenic 50.0%
GCCTATGAGGGATTTGAAGG+AGG - chr3.4:89760188-89760207 MS.gene012961:CDS 50.0%
GGCAATGTGACAGCCTATGA+GGG - chr3.4:89760176-89760195 MS.gene012961:CDS 50.0%
GTGACAAGACGGCCAAAAAG+AGG + chr3.4:89759159-89759178 None:intergenic 50.0%
TCTCCGGTTGTCTACCAACT+TGG - chr3.4:89760032-89760051 MS.gene012961:CDS 50.0%
TGGCAATGTGACAGCCTATG+AGG - chr3.4:89760175-89760194 MS.gene012961:CDS 50.0%
! AGGAGGAGGAAGCATGGATT+TGG - chr3.4:89760205-89760224 MS.gene012961:CDS 50.0%
!! ATGATTTAGGTTGGGCAGCG+TGG - chr3.4:89759940-89759959 MS.gene012961:CDS 50.0%
!! CGACCAAGTTGGTAGACAAC+CGG + chr3.4:89760038-89760057 None:intergenic 50.0%
AAGTACTCTTGCGGGAGAGG+TGG + chr3.4:89759864-89759883 None:intergenic 55.0%
CAGGTCACTGAGCTAGTGGA+TGG - chr3.4:89759405-89759424 MS.gene012961:CDS 55.0%
GAGTGCGCTAGAATCAGACC+AGG - chr3.4:89760253-89760272 MS.gene012961:CDS 55.0%
! ACGATTGGCGGAGCATCAAC+CGG + chr3.4:89759318-89759337 None:intergenic 55.0%
! CCAAAGTACTCTTGCGGGAG+AGG + chr3.4:89759867-89759886 None:intergenic 55.0%
!! CCTCTCCCGCAAGAGTACTT+TGG - chr3.4:89759864-89759883 MS.gene012961:CDS 55.0%
!! TTTAGGTTGGGCAGCGTGGA+AGG - chr3.4:89759944-89759963 MS.gene012961:CDS 55.0%
! ATTGGCGGAGCATCAACCGG+TGG + chr3.4:89759315-89759334 None:intergenic 60.0%
! GGTTGATGCTCCGCCAATCG+TGG - chr3.4:89759317-89759336 MS.gene012961:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89758910 89760322 89758910 ID=MS.gene012961
chr3.4 mRNA 89758910 89760322 89758910 ID=MS.gene012961.t1;Parent=MS.gene012961
chr3.4 exon 89758910 89760322 89758910 ID=MS.gene012961.t1.exon1;Parent=MS.gene012961.t1
chr3.4 CDS 89758910 89760322 89758910 ID=cds.MS.gene012961.t1;Parent=MS.gene012961.t1
Gene Sequence

>MS.gene012961

ATGACTTCCTCTATCACTCAAAGAATTTCAGAATGTTATATCAAACCACTACACCCTATTATTGAAGATTCAAAGCAAATTTGTTACTTAACAGGCTGGGATATTGCTATGTCATCAATGCACTACATCCAAAAAGGCCTATTATTCAAAAAACCAACAACTTCACCAAATAACCAACAAGATTTCATTCAGAATCTATTGGAGAAGCTTAAACACTCCCTCTCTAATGCTCTTTTCCATTTCTATCCTCTTTTTGGCCGTCTTGTCACTCACAAAACTCAAGACCCTCCCTCTTATACCATGTTTGTTGATTGCAGTAACGATAACACCGGAGCTAGATTCATCTATGCAACTTTGGATGCAGCTATATATGATATCGATAAAGCAATCAATCAGGATGCTCCACCGGTTGATGCTCCGCCAATCGTGGACTCGTTGTTTGATCTTGATAGAGCAATCAATCATGATGGTCACACCATGCCGTTGCTATCCATTCAGGTCACTGAGCTAGTGGATGGTGTTTTCATAGGCTGTTCAATGAACCATTGTGTTGGTGATGGGACTTCTTTTTTGAATTTCTTCAATATATGGTCTGAAATATATCAAGCTCAAGCCAAAGGCGATGATGTTCTCATTTCGCACCAACCTTTTCATAATCGGTGGTTCCTAGAAGGTTACGGTCCACTAATTAATCTTCCGTTCAAACATTTTGACGAGTTTACAAGCAGATTTGAAGCAGTACCCAAATTGAGAGAGAAATTATTTCACTTTAGTGCAGAGTCTATTGCAAAACTGAAAGCAAAGGCCAATAAAGAATCCAATACAAATAAAATATCTTCCTTCCAATCTTTATCGGCATTTGTTTGGAGAAGTGTGACTCGTGCACGAAGACTAAAACATGATCAAAAAACCAGTTGCAAGTTAGCCATAAACAATAGATCAAGAATCAAACCACCTCTCCCGCAAGAGTACTTTGGAAACTCAGTTGACGTGGTAAGTACAAAAACGACTGTAGGCGAATTGCTTGAGAATGATTTAGGTTGGGCAGCGTGGAAGGTGCATACACTTGTTGTGAATCACGACGATAGAGAGGTGAGAGAGATGCTCAAAAATTGGTTAGAGTCTCCGGTTGTCTACCAACTTGGTCGCCATTTTGATCCCTTCAGCATGGTGATGTCAAGTTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGAAAAGCGGTTGCAGTTCTAAGTGGATATGCAAACAAATTTGATGGCAATGTGACAGCCTATGAGGGATTTGAAGGAGGAGGAAGCATGGATTTGGCAGTGTCCCTTTTGCCTAATGCAATGAGTGCGCTAGAATCAGACCAGGATTTTATGAATGCAATTTCTGTGGTCAATTTTTCTAATATTCTGTGA
Protein sequence

>MS.gene012961.t1

MTSSITQRISECYIKPLHPIIEDSKQICYLTGWDIAMSSMHYIQKGLLFKKPTTSPNNQQDFIQNLLEKLKHSLSNALFHFYPLFGRLVTHKTQDPPSYTMFVDCSNDNTGARFIYATLDAAIYDIDKAINQDAPPVDAPPIVDSLFDLDRAINHDGHTMPLLSIQVTELVDGVFIGCSMNHCVGDGTSFLNFFNIWSEIYQAQAKGDDVLISHQPFHNRWFLEGYGPLINLPFKHFDEFTSRFEAVPKLREKLFHFSAESIAKLKAKANKESNTNKISSFQSLSAFVWRSVTRARRLKHDQKTSCKLAINNRSRIKPPLPQEYFGNSVDVVSTKTTVGELLENDLGWAAWKVHTLVVNHDDREVREMLKNWLESPVVYQLGRHFDPFSMVMSSSPRFNMYGNEFGMGKAVAVLSGYANKFDGNVTAYEGFEGGGSMDLAVSLLPNAMSALESDQDFMNAISVVNFSNIL