AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32936

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32936.t1 MTR_3g101960 93.640 456 29 0 1 456 1 456 0.0 902
MS.gene32936.t1 MTR_3g102020 86.771 446 58 1 1 445 1 446 0.0 814
MS.gene32936.t1 MTR_3g101970 87.668 446 40 2 1 445 1 432 0.0 806
MS.gene32936.t1 MTR_3g102010 80.846 449 78 2 1 445 2 446 0.0 755
MS.gene32936.t1 MTR_3g101900 69.265 449 127 4 1 445 2 443 0.0 629
MS.gene32936.t1 MTR_3g101930 67.937 446 129 5 9 445 10 450 0.0 622
MS.gene32936.t1 MTR_3g102030 75.472 318 52 2 86 403 29 320 2.55e-174 491
MS.gene32936.t1 MTR_4g015180 40.808 495 222 11 5 445 5 482 2.61e-103 317
MS.gene32936.t1 MTR_3g070170 39.175 485 221 14 5 445 11 465 3.05e-100 308
MS.gene32936.t1 MTR_5g081960 39.148 493 232 15 5 445 5 481 2.56e-95 296
MS.gene32936.t1 MTR_4g094842 38.950 457 251 11 3 445 1 443 7.34e-94 291
MS.gene32936.t1 MTR_4g022720 40.000 430 238 9 21 445 16 430 1.10e-88 277
MS.gene32936.t1 MTR_4g094835 38.876 427 242 7 25 445 28 441 1.86e-88 277
MS.gene32936.t1 MTR_8g041770 39.524 420 238 10 29 445 30 436 6.64e-88 275
MS.gene32936.t1 MTR_4g094838 38.621 435 228 9 29 454 28 432 1.07e-87 275
MS.gene32936.t1 MTR_4g022800 38.673 437 241 10 21 445 16 437 3.01e-87 274
MS.gene32936.t1 MTR_0190s0020 37.915 422 246 9 28 445 28 437 7.60e-85 268
MS.gene32936.t1 MTR_4g022670 37.844 436 245 10 21 445 16 436 3.53e-83 263
MS.gene32936.t1 MTR_4g022825 37.300 437 247 10 20 445 15 435 5.95e-83 263
MS.gene32936.t1 MTR_3g088420 37.995 429 242 10 23 445 18 428 9.35e-82 259
MS.gene32936.t1 MTR_7g026860 37.300 437 243 12 21 445 16 433 1.01e-79 254
MS.gene32936.t1 MTR_4g094828 37.020 443 243 14 8 431 6 431 3.32e-78 251
MS.gene32936.t1 MTR_3g026310 37.296 429 231 12 29 437 24 434 1.17e-75 244
MS.gene32936.t1 MTR_4g022290 35.476 420 239 10 23 429 24 424 2.86e-75 243
MS.gene32936.t1 MTR_4g094832 40.282 355 197 6 105 454 86 430 1.09e-72 236
MS.gene32936.t1 MTR_8g079710 36.706 425 236 15 21 431 21 426 6.50e-72 234
MS.gene32936.t1 MTR_4g022760 37.183 355 200 8 21 364 16 358 9.37e-66 217
MS.gene32936.t1 MTR_6g045553 32.008 478 268 12 5 445 13 470 2.46e-62 210
MS.gene32936.t1 MTR_6g016815 29.343 426 217 11 23 445 32 376 9.11e-42 152
MS.gene32936.t1 MTR_8g040780 35.897 234 141 4 234 459 14 246 3.17e-41 147
MS.gene32936.t1 MTR_8g075610 27.041 392 243 15 68 440 47 414 2.95e-31 124
MS.gene32936.t1 MTR_4g007540 25.708 459 283 19 6 440 3 427 1.57e-30 123
MS.gene32936.t1 MTR_2g064495 25.791 411 269 15 48 440 34 426 3.16e-30 122
MS.gene32936.t1 MTR_2g105330 25.547 411 270 15 48 440 34 426 1.14e-29 120
MS.gene32936.t1 MTR_7g026850 42.953 149 74 4 43 190 21 159 3.79e-29 113
MS.gene32936.t1 MTR_8g096530 29.524 315 191 11 62 362 53 350 8.49e-29 118
MS.gene32936.t1 MTR_7g026770 37.853 177 89 3 155 331 132 287 2.91e-28 114
MS.gene32936.t1 MTR_3g098980 28.333 300 194 8 68 358 66 353 7.39e-27 112
MS.gene32936.t1 MTR_8g040760 34.091 220 128 6 8 223 6 212 2.98e-26 106
MS.gene32936.t1 MTR_4g127650 26.154 455 250 19 19 431 30 440 4.09e-26 110
MS.gene32936.t1 MTR_3g049150 29.032 310 178 12 56 344 46 334 7.66e-26 110
MS.gene32936.t1 MTR_4g094825 35.429 175 98 4 280 454 232 391 4.25e-25 107
MS.gene32936.t1 MTR_4g094825 30.901 233 130 8 29 244 92 310 7.15e-23 100
MS.gene32936.t1 MTR_4g097090 25.513 439 263 17 29 431 30 440 8.66e-24 103
MS.gene32936.t1 MTR_3g026950 26.316 323 194 10 62 358 48 352 9.42e-24 103
MS.gene32936.t1 MTR_6g053810 31.271 291 174 13 68 344 71 349 1.39e-23 103
MS.gene32936.t1 MTR_5g076700 24.612 451 298 15 4 436 5 431 1.74e-23 103
MS.gene32936.t1 MTR_6g034495 39.189 148 82 5 49 190 13 158 5.73e-23 95.5
MS.gene32936.t1 MTR_7g027175 26.554 354 229 13 56 397 56 390 4.06e-22 99.0
MS.gene32936.t1 MTR_7g114470 25.926 432 266 18 33 436 28 433 6.07e-22 98.6
MS.gene32936.t1 MTR_1g040175 28.533 368 224 17 65 419 61 402 2.42e-21 96.7
MS.gene32936.t1 MTR_3g112180 26.904 394 251 13 18 399 22 390 1.02e-20 94.7
MS.gene32936.t1 MTR_7g114550 25.522 431 273 18 30 436 24 430 4.51e-20 92.8
MS.gene32936.t1 MTR_1g040195 24.938 405 265 14 50 436 21 404 6.61e-20 92.0
MS.gene32936.t1 MTR_3g465980 26.648 349 212 14 6 334 10 334 3.81e-19 90.1
MS.gene32936.t1 MTR_7g114560 25.522 431 273 18 30 436 24 430 9.90e-19 89.0
MS.gene32936.t1 MTR_1g040215 27.586 348 215 14 13 347 16 339 1.35e-18 88.6
MS.gene32936.t1 MTR_6g034485 37.607 117 66 2 205 317 2 115 1.63e-18 81.6
MS.gene32936.t1 MTR_3g071320 25.416 421 247 17 5 395 4 387 2.17e-18 87.4
MS.gene32936.t1 MTR_1g081660 26.736 288 191 10 65 344 70 345 2.23e-18 87.8
MS.gene32936.t1 MTR_7g114960 25.758 396 267 11 65 445 60 443 3.23e-18 87.0
MS.gene32936.t1 MTR_7g086000 26.303 403 235 18 71 433 63 443 9.96e-18 85.9
MS.gene32936.t1 MTR_4g055170 25.267 281 183 8 68 334 69 336 3.32e-17 84.3
MS.gene32936.t1 MTR_3g465990 26.074 349 214 14 6 334 10 334 4.16e-17 84.0
MS.gene32936.t1 MTR_3g465930 28.058 278 174 9 68 331 69 334 4.99e-17 83.6
MS.gene32936.t1 MTR_1g040315 27.541 305 198 10 30 325 23 313 9.78e-16 79.3
MS.gene32936.t1 MTR_7g084940 22.841 359 239 12 17 360 25 360 1.03e-15 79.3
MS.gene32936.t1 MTR_7g013850 23.095 420 263 13 30 402 30 436 4.06e-15 77.8
MS.gene32936.t1 MTR_7g026830 50.000 70 34 1 111 179 28 97 5.45e-15 70.9
MS.gene32936.t1 MTR_2g015430 24.585 301 205 10 68 358 58 346 1.69e-14 75.9
MS.gene32936.t1 MTR_3g465950 27.240 279 174 8 69 331 69 334 3.52e-14 74.7
MS.gene32936.t1 MTR_5g090830 23.292 322 197 13 48 342 49 347 1.10e-13 73.2
MS.gene32936.t1 MTR_2g045540 35.227 88 49 1 242 329 27 106 2.41e-13 66.6
MS.gene32936.t1 MTR_7g014620 23.007 439 285 19 29 441 15 426 2.51e-13 72.0
MS.gene32936.t1 MTR_3g028210 23.769 467 299 17 6 436 8 453 2.89e-13 72.0
MS.gene32936.t1 MTR_5g090810 23.292 322 197 14 48 342 49 347 3.45e-13 71.6
MS.gene32936.t1 MTR_8g079970 26.176 340 208 15 6 330 3 314 1.82e-12 69.3
MS.gene32936.t1 MTR_3g049140 24.631 203 137 4 242 431 27 226 2.29e-12 67.4
MS.gene32936.t1 MTR_5g090880 23.057 386 243 17 48 399 49 414 2.51e-12 68.9
MS.gene32936.t1 MTR_0502s0010 29.832 238 147 11 111 337 2 230 3.73e-12 66.6
MS.gene32936.t1 MTR_7g085930 25.291 344 206 14 50 367 45 363 4.10e-12 68.6
MS.gene32936.t1 MTR_7g085990 25.444 338 205 14 50 363 41 355 4.84e-12 68.2
MS.gene32936.t1 MTR_2g089755 22.628 411 267 14 29 402 37 433 4.88e-12 68.2
MS.gene32936.t1 MTR_7g014200 23.571 420 278 17 15 402 16 424 7.79e-12 67.4
MS.gene32936.t1 MTR_7g014630 29.557 203 129 7 14 209 15 210 1.06e-11 66.6
MS.gene32936.t1 MTR_6g015320 23.874 444 275 18 29 438 29 443 1.37e-11 66.6
MS.gene32936.t1 MTR_7g015860 21.718 419 247 16 29 399 28 413 5.92e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32936.t1 AT5G42830 56.818 440 174 4 13 445 14 444 0.0 514
MS.gene32936.t1 AT5G07850 54.649 441 199 1 6 445 8 448 1.70e-178 508
MS.gene32936.t1 AT5G07860 53.378 444 196 5 6 445 8 444 2.88e-164 471
MS.gene32936.t1 AT5G07870 50.331 453 208 3 6 445 8 456 4.84e-161 463
MS.gene32936.t1 AT2G39980 40.795 478 215 9 17 445 17 475 4.76e-105 321
MS.gene32936.t1 AT5G67150 40.045 447 247 11 8 445 7 441 2.13e-99 305
MS.gene32936.t1 AT5G01210 39.875 479 231 11 5 445 5 464 3.82e-96 298
MS.gene32936.t1 AT3G50270 35.938 448 265 11 8 445 8 443 2.48e-81 259
MS.gene32936.t1 AT3G50300 35.484 434 261 9 6 432 4 425 4.37e-78 250
MS.gene32936.t1 AT3G50280 35.385 455 273 11 3 449 1 442 4.88e-78 250
MS.gene32936.t1 AT5G38130 34.703 438 265 11 2 432 20 443 3.90e-72 235
MS.gene32936.t1 AT5G67160 34.753 446 264 12 6 446 5 428 2.59e-71 232
MS.gene32936.t1 AT5G23940 33.260 457 276 12 6 445 3 447 2.91e-65 218
MS.gene32936.t1 AT3G50280 38.312 308 179 6 144 445 6 308 1.39e-55 187
MS.gene32936.t1 AT3G50290 40.467 257 144 6 147 396 15 269 4.69e-47 164
MS.gene32936.t1 AT5G48930 26.258 457 283 17 6 440 3 427 2.76e-32 128
MS.gene32936.t1 AT2G19070 27.540 443 259 18 29 436 25 440 4.03e-29 119
MS.gene32936.t1 AT5G41040 28.339 307 200 7 62 358 63 359 1.88e-27 114
MS.gene32936.t1 AT5G41040 28.339 307 200 7 62 358 79 375 2.91e-27 114
MS.gene32936.t1 AT3G48720 26.634 413 259 16 18 420 18 396 2.85e-24 105
MS.gene32936.t1 AT2G40230 26.933 375 215 13 63 409 54 397 3.14e-21 96.3
MS.gene32936.t1 AT5G63560 27.246 345 209 13 28 358 29 345 4.12e-21 95.9
MS.gene32936.t1 AT1G28680 24.590 366 249 12 3 358 1 349 8.51e-20 91.7
MS.gene32936.t1 AT1G28680 24.590 366 249 12 3 358 1 349 1.58e-19 91.3
MS.gene32936.t1 AT1G31490 25.485 412 254 18 58 446 52 433 9.70e-18 85.9
MS.gene32936.t1 AT1G78990 23.548 310 194 12 48 342 52 333 3.54e-17 84.0
MS.gene32936.t1 AT1G32910 23.397 312 195 13 48 342 52 336 1.52e-16 82.0
MS.gene32936.t1 AT2G25150 26.557 305 182 13 61 344 60 343 1.07e-13 73.6
MS.gene32936.t1 AT5G16410 22.189 338 207 12 24 342 56 356 3.94e-13 71.6
MS.gene32936.t1 AT1G03390 24.566 403 255 19 53 431 64 441 8.47e-13 70.5
MS.gene32936.t1 AT1G65450 27.419 186 113 6 166 335 1 180 8.16e-12 66.2
MS.gene32936.t1 AT1G03495 24.719 356 213 15 7 326 9 345 2.26e-11 65.5
MS.gene32936.t1 AT5G39080 21.769 441 269 14 3 397 2 412 2.78e-11 65.9

Find 116 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.1:-80956028 MS.gene32936:CDS
CAAAATGATGTTCCTGTTTA+TGG 0.097445 3.1:-80956373 MS.gene32936:CDS
GAAGATTCTCTCTCTTAAAA+TGG 0.174060 3.1:+80956240 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.204491 3.1:-80955788 MS.gene32936:CDS
AATGTGTTAAATAACATATT+TGG 0.276151 3.1:+80956875 None:intergenic
TTGAATGGAAGGTTGATAAT+TGG 0.293176 3.1:+80956298 None:intergenic
GGGAGTGAGGATATCATTTA+TGG 0.298715 3.1:+80956591 None:intergenic
TCACCAAGGTTTAATATGTA+TGG 0.306646 3.1:-80955800 MS.gene32936:CDS
AATCTTTATCAGCATTAGTT+TGG 0.307683 3.1:-80956138 MS.gene32936:CDS
ATTTGGTTTGAGTCTTTGAT+TGG 0.311493 3.1:+80956892 None:intergenic
CTATAGACTCAGCTGGAAAA+TGG 0.328484 3.1:+80956218 None:intergenic
TCTTGAAGAGAAGTCCCTTT+TGG 0.328911 3.1:+80956821 None:intergenic
AACATGCAGAAACGATGTTT+AGG 0.345991 3.1:+80956932 None:intergenic
AGATGGTTTCCACAAGGTTA+TGG 0.364094 3.1:-80956322 MS.gene32936:CDS
TTGCTCCCATGTTCTAGTCT+TGG 0.366045 3.1:-80955599 MS.gene32936:CDS
AATGGAAAAGTGTGAGAGAA+AGG 0.389200 3.1:+80956734 None:intergenic
TTTGGTTTGAGTCTTTGATT+GGG 0.389491 3.1:+80956893 None:intergenic
CTTCGTCCTCCTTCTTTACC+TGG 0.390289 3.1:+80955698 None:intergenic
GCCGGAGAAAATGATGTTGC+TGG 0.398572 3.1:-80955651 MS.gene32936:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3.1:+80956441 None:intergenic
TTGGTGATGGAACAGCTTAT+TGG 0.406142 3.1:-80956426 MS.gene32936:CDS
TTTCCATTTCTATCCCCTCT+CGG 0.413697 3.1:-80956719 MS.gene32936:CDS
AATATGTTATTTAACACATT+GGG 0.417754 3.1:-80956872 MS.gene32936:CDS
ACATGCAGAAACGATGTTTA+GGG 0.418061 3.1:+80956933 None:intergenic
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.1:+80956348 None:intergenic
CAGATGAAGAGTTCAACTTC+TGG 0.424021 3.1:-80955625 MS.gene32936:CDS
ATTTGGAATGGGAAAAGCTT+TGG 0.427618 3.1:-80955771 MS.gene32936:CDS
GGAGAGAACAACTTGCAAAA+TGG 0.431719 3.1:-80956080 MS.gene32936:CDS
ATCAACCTATCCACAATAGA+TGG 0.431811 3.1:-80956339 MS.gene32936:CDS
GACAATGGGTGGGACGTCAA+TGG 0.437426 3.1:+80956570 None:intergenic
TTCCATTTCTATCCCCTCTC+GGG 0.439902 3.1:-80956718 MS.gene32936:CDS
TCTAGTCTTGGAGTATGTTA+TGG 0.446784 3.1:-80955587 MS.gene32936:CDS
ACAATGGGTGGGACGTCAAT+GGG 0.448722 3.1:+80956571 None:intergenic
TGTGAAATGGGACCATAAAC+AGG 0.450206 3.1:+80956361 None:intergenic
TATTCAAGTCACGGAATTAG+TGG 0.454807 3.1:-80956485 MS.gene32936:CDS
TGATCCTTGCACTGTTATGA+TGG 0.455482 3.1:-80955828 MS.gene32936:CDS
ATCTACACACTATATCCAAA+AGG 0.455900 3.1:-80956836 MS.gene32936:CDS
TACAAATAACCGTACGAGAA+TGG 0.458161 3.1:-80956056 MS.gene32936:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.1:+80956675 None:intergenic
TCAAAGAACGATTGGACAAT+GGG 0.466912 3.1:+80956556 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.1:+80956028 None:intergenic
AAGATTCTCTCTCTTAAAAT+GGG 0.467841 3.1:+80956241 None:intergenic
GAATTCCCAAAATATTCCTT+AGG 0.480035 3.1:+80956022 None:intergenic
TGTTCCATGAATCATTGTAT+TGG 0.480232 3.1:-80956445 MS.gene32936:CDS
AAATATGTTATTTAACACAT+TGG 0.480800 3.1:-80956873 MS.gene32936:CDS
AACTCATCGTGATGTTTGAA+TGG 0.482751 3.1:+80956283 None:intergenic
GACCCGAGAGGGGATAGAAA+TGG 0.492196 3.1:+80956716 None:intergenic
AGTTTCGCTATAGACTCAGC+TGG 0.493398 3.1:+80956211 None:intergenic
GAATTGCTGGAGAATGATCT+AGG 0.495485 3.1:-80955965 MS.gene32936:CDS
CACCAAGGTTTAATATGTAT+GGG 0.497351 3.1:-80955799 MS.gene32936:CDS
ATGGAAAAGTGTGAGAGAAA+GGG 0.500507 3.1:+80956735 None:intergenic
TTCCCATACATATTAAACCT+TGG 0.501336 3.1:+80955797 None:intergenic
GTGCACGTCAACTACAACAC+CGG 0.505056 3.1:-80956102 MS.gene32936:CDS
TTGGTTTGAGTCTTTGATTG+GGG 0.506402 3.1:+80956894 None:intergenic
TGTTGGTTTAGTGATGATGT+TGG 0.506905 3.1:+80956796 None:intergenic
GACAAAGCAGTCAGCCATGA+TGG 0.509313 3.1:-80956529 MS.gene32936:CDS
AGTGTTTGAGTTTGTCCACC+AGG 0.515596 3.1:+80956758 None:intergenic
TTTGTTATCTATTCAAGTCA+CGG 0.519202 3.1:-80956494 MS.gene32936:CDS
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.1:-80955782 MS.gene32936:CDS
TGCTGGAGAATGATCTAGGA+TGG 0.523136 3.1:-80955961 MS.gene32936:CDS
GGTTCTCAATGAAGTGCTGT+TGG 0.527425 3.1:+80956779 None:intergenic
TGTCATGATCAAAGAACGAT+TGG 0.527909 3.1:+80956548 None:intergenic
AAATCTGTTTGATAAACAAA+AGG 0.527952 3.1:+80955857 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.1:-80956439 MS.gene32936:CDS
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.1:+80956349 None:intergenic
ACATCATATCCAGGTAAAGA+AGG 0.534700 3.1:-80955707 MS.gene32936:CDS
CATCGTGATGTTTGAATGGA+AGG 0.541756 3.1:+80956287 None:intergenic
GGAAAAGTAACATCATATCC+AGG 0.542909 3.1:-80955716 MS.gene32936:CDS
GGATATGCTAACAAATTCGA+TGG 0.547641 3.1:-80955737 MS.gene32936:CDS
GATCCTTGCACTGTTATGAT+GGG 0.548259 3.1:-80955827 MS.gene32936:CDS
TCTTGAAGTGTGTCTTTCGC+CGG 0.548668 3.1:-80955669 MS.gene32936:CDS
TGCGGTGAGTGCTGAAACGA+CGG 0.549616 3.1:-80955993 MS.gene32936:CDS
AATAAATCATGATGATAAAG+TGG 0.561465 3.1:-80955912 MS.gene32936:CDS
AAAGCTTTGGCAGTTAGAAG+TGG 0.567341 3.1:-80955758 MS.gene32936:CDS
TCTACACACTATATCCAAAA+GGG 0.567974 3.1:-80956835 MS.gene32936:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.1:-80955952 MS.gene32936:CDS
ATCAAAGAACGATTGGACAA+TGG 0.570569 3.1:+80956555 None:intergenic
AATGGAACCACCTCTTCCTA+AGG 0.571584 3.1:-80956038 MS.gene32936:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.1:-80955783 MS.gene32936:CDS
ACAGCACTTCATTGAGAACC+TGG 0.575720 3.1:-80956776 MS.gene32936:CDS
TGGTTTGAGTCTTTGATTGG+GGG 0.580337 3.1:+80956895 None:intergenic
GAGTGCTGAAACGACGGTGG+GGG 0.585490 3.1:-80955987 MS.gene32936:CDS
ACGAAAACAGTGTAAGAATG+AGG 0.585728 3.1:+80956667 None:intergenic
CACAATAGATGGTTTCCACA+AGG 0.586552 3.1:-80956328 MS.gene32936:CDS
CAGAAACGATGTTTAGGGAA+GGG 0.589656 3.1:+80956938 None:intergenic
GAGGTGGTTCCATTCTCGTA+CGG 0.591000 3.1:+80956047 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.1:+80956031 None:intergenic
TGCGTAGGTCGGTTGAAGAG+TGG 0.594966 3.1:-80955889 MS.gene32936:CDS
TGCACGTCAACTACAACACC+GGG 0.595711 3.1:-80956101 MS.gene32936:CDS
GTGAGTGCTGAAACGACGGT+GGG 0.603452 3.1:-80955989 MS.gene32936:CDS
AGTAACAAGACGACCCGAGA+GGG 0.603722 3.1:+80956705 None:intergenic
CATACTCCAAGACTAGAACA+TGG 0.604636 3.1:+80955593 None:intergenic
GCAGAAACGATGTTTAGGGA+AGG 0.605789 3.1:+80956937 None:intergenic
ATACTCCAAGACTAGAACAT+GGG 0.613734 3.1:+80955594 None:intergenic
TGAGTGCTGAAACGACGGTG+GGG 0.614267 3.1:-80955988 MS.gene32936:CDS
CTACCCATCATAACAGTGCA+AGG 0.614444 3.1:+80955824 None:intergenic
AAGAACGATTGGACAATGGG+TGG 0.614907 3.1:+80956559 None:intergenic
AAGACATGGTATGACCATCA+TGG 0.620179 3.1:+80956515 None:intergenic
GGAAACCATCTATTGTGGAT+AGG 0.621301 3.1:+80956334 None:intergenic
GATATAGTGTGTAGATAACA+TGG 0.622938 3.1:+80956843 None:intergenic
AGAACGATTGGACAATGGGT+GGG 0.626217 3.1:+80956560 None:intergenic
GCTGGAGAATGATCTAGGAT+GGG 0.628974 3.1:-80955960 MS.gene32936:CDS
GTGGGACGTCAATGGGAGTG+AGG 0.636734 3.1:+80956578 None:intergenic
ATGATGATAAAGTGGTGCGT+AGG 0.641507 3.1:-80955904 MS.gene32936:CDS
ATAATTGGACCATAACCTTG+TGG 0.645522 3.1:+80956313 None:intergenic
TATAGCGAAACTGAAAGCAA+AGG 0.650352 3.1:-80956200 MS.gene32936:CDS
TCATATCCAGGTAAAGAAGG+AGG 0.654196 3.1:-80955704 MS.gene32936:CDS
CTTGTGGAAACCATCTATTG+TGG 0.654333 3.1:+80956329 None:intergenic
TGATAAAGTGGTGCGTAGGT+CGG 0.663573 3.1:-80955900 MS.gene32936:CDS
GGTGAGTGCTGAAACGACGG+TGG 0.672156 3.1:-80955990 MS.gene32936:CDS
CAAGTCACGGAATTAGTGGA+CGG 0.679556 3.1:-80956481 MS.gene32936:CDS
GTAACAAGACGACCCGAGAG+GGG 0.682817 3.1:+80956706 None:intergenic
TTGAATAGATAACAAAGACA+TGG 0.705301 3.1:+80956501 None:intergenic
TTATGATGGGTAGTTCACCA+AGG 0.706961 3.1:-80955814 MS.gene32936:CDS
GCACTTCATTGAGAACCTGG+TGG 0.715971 3.1:-80956773 MS.gene32936:CDS
TAGTAACAAGACGACCCGAG+AGG 0.734460 3.1:+80956704 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTGTTAAATAACATATT+TGG + chr3.1:80955654-80955673 None:intergenic 15.0%
!!! AAATATGTTATTTAACACAT+TGG - chr3.1:80955653-80955672 MS.gene32936:CDS 15.0%
!!! AATATGTTATTTAACACATT+GGG - chr3.1:80955654-80955673 MS.gene32936:CDS 15.0%
!! AAATCTGTTTGATAAACAAA+AGG + chr3.1:80956672-80956691 None:intergenic 20.0%
!! AATAAATCATGATGATAAAG+TGG - chr3.1:80956614-80956633 MS.gene32936:CDS 20.0%
! AAGATTCTCTCTCTTAAAAT+GGG + chr3.1:80956288-80956307 None:intergenic 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.1:80956738-80956757 MS.gene32936:CDS 25.0%
! AATCTTTATCAGCATTAGTT+TGG - chr3.1:80956388-80956407 MS.gene32936:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.1:80956028-80956047 None:intergenic 25.0%
!! TTTGTTATCTATTCAAGTCA+CGG - chr3.1:80956032-80956051 MS.gene32936:CDS 25.0%
ATCACCAATACAATGATTCA+TGG + chr3.1:80956088-80956107 None:intergenic 30.0%
ATCTACACACTATATCCAAA+AGG - chr3.1:80955690-80955709 MS.gene32936:CDS 30.0%
CAAAATGATGTTCCTGTTTA+TGG - chr3.1:80956153-80956172 MS.gene32936:CDS 30.0%
CACCAAGGTTTAATATGTAT+GGG - chr3.1:80956727-80956746 MS.gene32936:CDS 30.0%
GAAGATTCTCTCTCTTAAAA+TGG + chr3.1:80956289-80956308 None:intergenic 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.1:80956507-80956526 None:intergenic 30.0%
GATATAGTGTGTAGATAACA+TGG + chr3.1:80955686-80955705 None:intergenic 30.0%
TCACCAAGGTTTAATATGTA+TGG - chr3.1:80956726-80956745 MS.gene32936:CDS 30.0%
TCTACACACTATATCCAAAA+GGG - chr3.1:80955691-80955710 MS.gene32936:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.1:80956081-80956100 MS.gene32936:CDS 30.0%
TTCCCATACATATTAAACCT+TGG + chr3.1:80956732-80956751 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.1:80956087-80956106 MS.gene32936:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.1:80956499-80956518 MS.gene32936:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.1:80956744-80956763 MS.gene32936:CDS 30.0%
!! ATTTGGTTTGAGTCTTTGAT+TGG + chr3.1:80955637-80955656 None:intergenic 30.0%
!! TTGAATGGAAGGTTGATAAT+TGG + chr3.1:80956231-80956250 None:intergenic 30.0%
!! TTTGGTTTGAGTCTTTGATT+GGG + chr3.1:80955636-80955655 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.1:80956498-80956517 None:intergenic 35.0%
AACATGCAGAAACGATGTTT+AGG + chr3.1:80955597-80955616 None:intergenic 35.0%
AACTCATCGTGATGTTTGAA+TGG + chr3.1:80956246-80956265 None:intergenic 35.0%
ACATCATATCCAGGTAAAGA+AGG - chr3.1:80956819-80956838 MS.gene32936:CDS 35.0%
ACATGCAGAAACGATGTTTA+GGG + chr3.1:80955596-80955615 None:intergenic 35.0%
ACGAAAACAGTGTAAGAATG+AGG + chr3.1:80955862-80955881 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.1:80955854-80955873 None:intergenic 35.0%
ATAATTGGACCATAACCTTG+TGG + chr3.1:80956216-80956235 None:intergenic 35.0%
ATACTCCAAGACTAGAACAT+GGG + chr3.1:80956935-80956954 None:intergenic 35.0%
ATCAAAGAACGATTGGACAA+TGG + chr3.1:80955974-80955993 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.1:80956187-80956206 MS.gene32936:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.1:80956501-80956520 None:intergenic 35.0%
GGAAAAGTAACATCATATCC+AGG - chr3.1:80956810-80956829 MS.gene32936:CDS 35.0%
GGATATGCTAACAAATTCGA+TGG - chr3.1:80956789-80956808 MS.gene32936:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.1:80956743-80956762 MS.gene32936:CDS 35.0%
TACAAATAACCGTACGAGAA+TGG - chr3.1:80956470-80956489 MS.gene32936:CDS 35.0%
TATAGCGAAACTGAAAGCAA+AGG - chr3.1:80956326-80956345 MS.gene32936:CDS 35.0%
TATTCAAGTCACGGAATTAG+TGG - chr3.1:80956041-80956060 MS.gene32936:CDS 35.0%
TCAAAGAACGATTGGACAAT+GGG + chr3.1:80955973-80955992 None:intergenic 35.0%
TGTCATGATCAAAGAACGAT+TGG + chr3.1:80955981-80956000 None:intergenic 35.0%
! AATGGAAAAGTGTGAGAGAA+AGG + chr3.1:80955795-80955814 None:intergenic 35.0%
! ATGGAAAAGTGTGAGAGAAA+GGG + chr3.1:80955794-80955813 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCTT+TGG - chr3.1:80956755-80956774 MS.gene32936:CDS 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.1:80956498-80956517 MS.gene32936:CDS 35.0%
! TTGGTTTGAGTCTTTGATTG+GGG + chr3.1:80955635-80955654 None:intergenic 35.0%
!! TCTAGTCTTGGAGTATGTTA+TGG - chr3.1:80956939-80956958 MS.gene32936:CDS 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.1:80956180-80956199 None:intergenic 35.0%
!! TGTTGGTTTAGTGATGATGT+TGG + chr3.1:80955733-80955752 None:intergenic 35.0%
!! TTTTGGGAATTCACTTCATG+CGG - chr3.1:80956515-80956534 MS.gene32936:CDS 35.0%
AAGACATGGTATGACCATCA+TGG + chr3.1:80956014-80956033 None:intergenic 40.0%
AGATGGTTTCCACAAGGTTA+TGG - chr3.1:80956204-80956223 MS.gene32936:CDS 40.0%
ATGATGATAAAGTGGTGCGT+AGG - chr3.1:80956622-80956641 MS.gene32936:CDS 40.0%
CACAATAGATGGTTTCCACA+AGG - chr3.1:80956198-80956217 MS.gene32936:CDS 40.0%
CAGAAACGATGTTTAGGGAA+GGG + chr3.1:80955591-80955610 None:intergenic 40.0%
CAGATGAAGAGTTCAACTTC+TGG - chr3.1:80956901-80956920 MS.gene32936:CDS 40.0%
CATACTCCAAGACTAGAACA+TGG + chr3.1:80956936-80956955 None:intergenic 40.0%
CATCGTGATGTTTGAATGGA+AGG + chr3.1:80956242-80956261 None:intergenic 40.0%
CTATAGACTCAGCTGGAAAA+TGG + chr3.1:80956311-80956330 None:intergenic 40.0%
CTTGTGGAAACCATCTATTG+TGG + chr3.1:80956200-80956219 None:intergenic 40.0%
GAATTGCTGGAGAATGATCT+AGG - chr3.1:80956561-80956580 MS.gene32936:CDS 40.0%
GATCCTTGCACTGTTATGAT+GGG - chr3.1:80956699-80956718 MS.gene32936:CDS 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.1:80956195-80956214 None:intergenic 40.0%
GGAGAGAACAACTTGCAAAA+TGG - chr3.1:80956446-80956465 MS.gene32936:CDS 40.0%
GGGAGTGAGGATATCATTTA+TGG + chr3.1:80955938-80955957 None:intergenic 40.0%
TCATATCCAGGTAAAGAAGG+AGG - chr3.1:80956822-80956841 MS.gene32936:CDS 40.0%
TGATCCTTGCACTGTTATGA+TGG - chr3.1:80956698-80956717 MS.gene32936:CDS 40.0%
TGGTTTGAGTCTTTGATTGG+GGG + chr3.1:80955634-80955653 None:intergenic 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.1:80956168-80956187 None:intergenic 40.0%
TTTCCATTTCTATCCCCTCT+CGG - chr3.1:80955807-80955826 MS.gene32936:CDS 40.0%
! AAAGCTTTGGCAGTTAGAAG+TGG - chr3.1:80956768-80956787 MS.gene32936:CDS 40.0%
! TCCAGCAACATCATTTTCTC+CGG + chr3.1:80956879-80956898 None:intergenic 40.0%
! TCTTGAAGAGAAGTCCCTTT+TGG + chr3.1:80955708-80955727 None:intergenic 40.0%
! TTTTGCAAGTTGTTCTCTCC+CGG + chr3.1:80956446-80956465 None:intergenic 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.1:80956181-80956200 None:intergenic 40.0%
!! TTAGTGGACGGTGTTTTCAT+TGG - chr3.1:80956057-80956076 MS.gene32936:CDS 40.0%
!! TTATGATGGGTAGTTCACCA+AGG - chr3.1:80956712-80956731 MS.gene32936:CDS 40.0%
!! TTGGTGATGGAACAGCTTAT+TGG - chr3.1:80956100-80956119 MS.gene32936:CDS 40.0%
AAGAACGATTGGACAATGGG+TGG + chr3.1:80955970-80955989 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.1:80956488-80956507 MS.gene32936:CDS 45.0%
ACAGCACTTCATTGAGAACC+TGG - chr3.1:80955750-80955769 MS.gene32936:CDS 45.0%
AGAACGATTGGACAATGGGT+GGG + chr3.1:80955969-80955988 None:intergenic 45.0%
AGTTTCGCTATAGACTCAGC+TGG + chr3.1:80956318-80956337 None:intergenic 45.0%
CAAGTCACGGAATTAGTGGA+CGG - chr3.1:80956045-80956064 MS.gene32936:CDS 45.0%
CTACCCATCATAACAGTGCA+AGG + chr3.1:80956705-80956724 None:intergenic 45.0%
GAGTCTTTGATTGGGGGATA+CGG + chr3.1:80955628-80955647 None:intergenic 45.0%
GCAGAAACGATGTTTAGGGA+AGG + chr3.1:80955592-80955611 None:intergenic 45.0%
GTCGATTGCAAAAACAGTCG+CGG - chr3.1:80955880-80955899 MS.gene32936:CDS 45.0%
TCTTGAAGTGTGTCTTTCGC+CGG - chr3.1:80956857-80956876 MS.gene32936:CDS 45.0%
TGATAAAGTGGTGCGTAGGT+CGG - chr3.1:80956626-80956645 MS.gene32936:CDS 45.0%
TGCTGGAGAATGATCTAGGA+TGG - chr3.1:80956565-80956584 MS.gene32936:CDS 45.0%
TTCCATTTCTATCCCCTCTC+GGG - chr3.1:80955808-80955827 MS.gene32936:CDS 45.0%
TTGCTCCCATGTTCTAGTCT+TGG - chr3.1:80956927-80956946 MS.gene32936:CDS 45.0%
! AGTGTTTGAGTTTGTCCACC+AGG + chr3.1:80955771-80955790 None:intergenic 45.0%
! GCTGGAGAATGATCTAGGAT+GGG - chr3.1:80956566-80956585 MS.gene32936:CDS 45.0%
!! GGTTCTCAATGAAGTGCTGT+TGG + chr3.1:80955750-80955769 None:intergenic 45.0%
ACAATGGGTGGGACGTCAAT+GGG + chr3.1:80955958-80955977 None:intergenic 50.0%
AGTAACAAGACGACCCGAGA+GGG + chr3.1:80955824-80955843 None:intergenic 50.0%
CTTCGTCCTCCTTCTTTACC+TGG + chr3.1:80956831-80956850 None:intergenic 50.0%
GACAAAGCAGTCAGCCATGA+TGG - chr3.1:80955997-80956016 MS.gene32936:CDS 50.0%
GAGGTGGTTCCATTCTCGTA+CGG + chr3.1:80956482-80956501 None:intergenic 50.0%
GCACTTCATTGAGAACCTGG+TGG - chr3.1:80955753-80955772 MS.gene32936:CDS 50.0%
GCCGGAGAAAATGATGTTGC+TGG - chr3.1:80956875-80956894 MS.gene32936:CDS 50.0%
GTGCACGTCAACTACAACAC+CGG - chr3.1:80956424-80956443 MS.gene32936:CDS 50.0%
TAGTAACAAGACGACCCGAG+AGG + chr3.1:80955825-80955844 None:intergenic 50.0%
TGCACGTCAACTACAACACC+GGG - chr3.1:80956425-80956444 MS.gene32936:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.1:80956574-80956593 MS.gene32936:CDS 50.0%
GACAATGGGTGGGACGTCAA+TGG + chr3.1:80955959-80955978 None:intergenic 55.0%
GACCCGAGAGGGGATAGAAA+TGG + chr3.1:80955813-80955832 None:intergenic 55.0%
GTAACAAGACGACCCGAGAG+GGG + chr3.1:80955823-80955842 None:intergenic 55.0%
TGCGTAGGTCGGTTGAAGAG+TGG - chr3.1:80956637-80956656 MS.gene32936:CDS 55.0%
!! GTGAGTGCTGAAACGACGGT+GGG - chr3.1:80956537-80956556 MS.gene32936:CDS 55.0%
!! TGAGTGCTGAAACGACGGTG+GGG - chr3.1:80956538-80956557 MS.gene32936:CDS 55.0%
!! TGCGGTGAGTGCTGAAACGA+CGG - chr3.1:80956533-80956552 MS.gene32936:CDS 55.0%
AACGACGGTGGGGGAATTGC+TGG - chr3.1:80956548-80956567 MS.gene32936:CDS 60.0%
GTGGGACGTCAATGGGAGTG+AGG + chr3.1:80955951-80955970 None:intergenic 60.0%
!! GAGTGCTGAAACGACGGTGG+GGG - chr3.1:80956539-80956558 MS.gene32936:CDS 60.0%
!! GGTGAGTGCTGAAACGACGG+TGG - chr3.1:80956536-80956555 MS.gene32936:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 80955580 80956968 80955580 ID=MS.gene32936
chr3.1 mRNA 80955580 80956968 80955580 ID=MS.gene32936.t1;Parent=MS.gene32936
chr3.1 exon 80955580 80956968 80955580 ID=MS.gene32936.t1.exon1;Parent=MS.gene32936.t1
chr3.1 CDS 80955580 80956968 80955580 ID=cds.MS.gene32936.t1;Parent=MS.gene32936.t1
Gene Sequence

>MS.gene32936

ATGAGGATCCCTTCCCTAAACATCGTTTCTGCATGTTTTATTAAACCGTATCCCCCAATCAAAGACTCAAACCAAATATGTTATTTAACACATTGGGATATTGCCATGTTATCTACACACTATATCCAAAAGGGACTTCTCTTCAAGAAACCAACATCATCACTAAACCAACAGCACTTCATTGAGAACCTGGTGGACAAACTCAAACACTCCCTTTCTCTCACACTTTTCCATTTCTATCCCCTCTCGGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTCGTCGATTGCAAAAACAGTCGCGGAGCTAAATTTATTCATGCAACTTTAGATATCACCATAAATGATATCCTCACTCCCATTGACGTCCCACCCATTGTCCAATCGTTCTTTGATCATGACAAAGCAGTCAGCCATGATGGTCATACCATGTCTTTGTTATCTATTCAAGTCACGGAATTAGTGGACGGTGTTTTCATTGGTTGTTCCATGAATCATTGTATTGGTGATGGAACAGCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATGTTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCACAAGGTTATGGTCCAATTATCAACCTTCCATTCAAACATCACGATGAGTTTGTTCACAGATATGAAACACCCATTTTAAGAGAGAGAATCTTCCATTTTCCAGCTGAGTCTATAGCGAAACTGAAAGCAAAGGCAAATGAAGAGTCTAATACAAGCAAAATTTCTTCGTTTCAATCTTTATCAGCATTAGTTTGGAGATCTATAACTCGTGCACGTCAACTACAACACCGGGAGAGAACAACTTGCAAAATGGCTACAAATAACCGTACGAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCACTTCATGCGGTGAGTGCTGAAACGACGGTGGGGGAATTGCTGGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATCTAGCTGTAATAAATCATGATGATAAAGTGGTGCGTAGGTCGGTTGAAGAGTGGTTTCGTTCTCCTTTTGTTTATCAAACAGATTTGTTTTTTGATCCTTGCACTGTTATGATGGGTAGTTCACCAAGGTTTAATATGTATGGGAATGAATTTGGAATGGGAAAAGCTTTGGCAGTTAGAAGTGGATATGCTAACAAATTCGATGGAAAAGTAACATCATATCCAGGTAAAGAAGGAGGACGAAGCATTGATCTTGAAGTGTGTCTTTCGCCGGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCAACTTCTGGTGTTTGCTCCCATGTTCTAGTCTTGGAGTATGTTATGGTGTTTAG
Protein sequence

>MS.gene32936.t1

MRIPSLNIVSACFIKPYPPIKDSNQICYLTHWDIAMLSTHYIQKGLLFKKPTSSLNQQHFIENLVDKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSRGAKFIHATLDITINDILTPIDVPPIVQSFFDHDKAVSHDGHTMSLLSIQVTELVDGVFIGCSMNHCIGDGTAYWNFFNTFSEIFQNDVPVYGPISHQPIHNRWFPQGYGPIINLPFKHHDEFVHRYETPILRERIFHFPAESIAKLKAKANEESNTSKISSFQSLSALVWRSITRARQLQHRERTTCKMATNNRTRMEPPLPKEYFGNSLHAVSAETTVGELLENDLGWAAWKIHLAVINHDDKVVRRSVEEWFRSPFVYQTDLFFDPCTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGKEGGRSIDLEVCLSPEKMMLLETDEEFNFWCLLPCSSLGVCYGV