AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32938

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32938.t1 MTR_3g101930 74.473 427 98 4 1 425 37 454 0.0 659
MS.gene32938.t1 MTR_3g101900 72.600 427 103 4 1 427 37 449 0.0 636
MS.gene32938.t1 MTR_3g102020 66.200 429 135 4 1 429 36 454 0.0 587
MS.gene32938.t1 MTR_3g101960 67.221 421 127 5 1 421 36 445 0.0 583
MS.gene32938.t1 MTR_3g102010 65.278 432 133 6 1 429 37 454 0.0 568
MS.gene32938.t1 MTR_3g101970 63.466 427 132 4 1 427 36 438 0.0 564
MS.gene32938.t1 MTR_3g102030 56.098 328 108 6 52 379 29 320 8.26e-129 375
MS.gene32938.t1 MTR_3g070170 36.659 461 229 8 1 427 40 471 1.88e-90 282
MS.gene32938.t1 MTR_4g015180 38.958 480 217 12 1 428 34 489 1.85e-89 280
MS.gene32938.t1 MTR_5g081960 36.134 476 223 11 1 422 34 482 2.39e-88 277
MS.gene32938.t1 MTR_4g094842 40.654 428 217 16 8 422 41 444 9.79e-88 274
MS.gene32938.t1 MTR_0190s0020 38.498 426 229 10 8 426 43 442 9.04e-84 264
MS.gene32938.t1 MTR_8g041770 37.055 421 240 8 8 426 44 441 2.01e-83 263
MS.gene32938.t1 MTR_4g022800 37.209 430 234 12 8 426 38 442 7.24e-83 261
MS.gene32938.t1 MTR_3g088420 38.263 426 226 10 8 426 38 433 2.24e-82 260
MS.gene32938.t1 MTR_4g022720 37.915 422 233 9 9 426 39 435 4.29e-81 257
MS.gene32938.t1 MTR_4g022670 37.355 431 231 12 8 426 38 441 5.01e-79 251
MS.gene32938.t1 MTR_4g094835 35.915 426 241 10 4 422 42 442 7.11e-79 251
MS.gene32938.t1 MTR_4g094838 34.895 427 234 10 4 422 38 428 1.30e-76 245
MS.gene32938.t1 MTR_4g022825 36.427 431 234 11 8 426 38 440 6.13e-76 243
MS.gene32938.t1 MTR_4g094828 35.616 438 243 12 4 427 39 451 4.22e-74 239
MS.gene32938.t1 MTR_7g026860 35.349 430 238 12 8 426 38 438 6.91e-73 236
MS.gene32938.t1 MTR_3g026310 36.758 438 231 13 8 426 38 448 3.51e-72 234
MS.gene32938.t1 MTR_4g094832 37.430 358 200 7 72 422 86 426 1.68e-68 224
MS.gene32938.t1 MTR_4g022290 33.256 430 246 10 7 424 43 443 5.29e-68 223
MS.gene32938.t1 MTR_8g079710 35.392 421 232 15 7 415 42 434 8.74e-63 209
MS.gene32938.t1 MTR_4g022760 36.443 343 186 9 8 340 38 358 6.74e-59 198
MS.gene32938.t1 MTR_6g045553 30.501 459 255 12 3 425 44 474 1.26e-53 186
MS.gene32938.t1 MTR_6g016815 30.521 403 200 12 24 426 59 381 2.93e-41 150
MS.gene32938.t1 MTR_2g064495 28.605 423 236 15 20 416 44 426 3.65e-37 140
MS.gene32938.t1 MTR_8g040780 37.321 209 127 3 216 421 20 227 4.01e-37 136
MS.gene32938.t1 MTR_2g105330 28.369 423 237 15 20 416 44 426 8.26e-37 140
MS.gene32938.t1 MTR_4g007540 28.916 415 228 16 20 407 44 418 1.62e-36 139
MS.gene32938.t1 MTR_8g075610 27.889 398 248 13 21 407 36 405 3.22e-35 135
MS.gene32938.t1 MTR_3g049150 26.914 405 248 11 21 401 45 425 1.75e-32 128
MS.gene32938.t1 MTR_8g096530 29.618 314 193 11 28 333 53 346 2.55e-31 125
MS.gene32938.t1 MTR_3g098980 31.419 296 174 11 34 321 66 340 3.45e-31 124
MS.gene32938.t1 MTR_3g026950 27.244 312 188 9 21 314 42 332 3.15e-29 119
MS.gene32938.t1 MTR_7g026850 39.873 158 75 4 8 164 21 159 1.58e-28 110
MS.gene32938.t1 MTR_7g026770 35.071 211 98 4 113 307 100 287 4.88e-28 113
MS.gene32938.t1 MTR_4g127650 26.116 448 235 17 14 416 53 449 4.81e-26 110
MS.gene32938.t1 MTR_4g097090 24.709 429 271 13 8 407 35 440 1.40e-23 102
MS.gene32938.t1 MTR_6g053810 26.032 315 198 9 35 337 72 363 1.86e-23 102
MS.gene32938.t1 MTR_4g094825 34.667 150 87 2 277 426 253 391 4.27e-23 100
MS.gene32938.t1 MTR_4g094825 34.969 163 92 4 4 166 102 250 6.23e-19 88.6
MS.gene32938.t1 MTR_6g034495 36.420 162 86 5 14 169 13 163 1.27e-21 91.7
MS.gene32938.t1 MTR_7g114550 25.926 405 261 15 31 422 62 440 2.38e-21 96.3
MS.gene32938.t1 MTR_3g071320 26.966 356 216 11 33 371 59 387 1.25e-20 94.0
MS.gene32938.t1 MTR_3g465980 28.571 294 176 10 29 310 63 334 1.60e-20 94.0
MS.gene32938.t1 MTR_7g027175 24.185 368 244 15 21 380 55 395 1.60e-20 93.6
MS.gene32938.t1 MTR_7g086000 27.007 411 246 17 37 416 63 450 4.59e-20 92.4
MS.gene32938.t1 MTR_7g085990 26.389 432 260 18 20 416 42 450 4.66e-20 92.4
MS.gene32938.t1 MTR_8g040760 34.896 192 104 5 8 198 41 212 9.10e-20 87.8
MS.gene32938.t1 MTR_3g087640 26.107 429 251 16 20 407 53 456 9.61e-20 91.7
MS.gene32938.t1 MTR_3g465990 27.891 294 178 10 29 310 63 334 1.38e-19 91.3
MS.gene32938.t1 MTR_7g114490 27.002 437 254 19 8 417 37 435 1.68e-19 90.9
MS.gene32938.t1 MTR_1g081660 27.451 357 217 16 31 375 70 396 2.27e-19 90.5
MS.gene32938.t1 MTR_4g013050 27.241 290 161 9 32 318 51 293 2.92e-19 89.7
MS.gene32938.t1 MTR_1g053315 25.542 415 251 20 19 407 63 445 5.75e-19 89.4
MS.gene32938.t1 MTR_1g040195 25.405 370 239 14 32 394 37 376 1.23e-18 88.2
MS.gene32938.t1 MTR_4g055170 26.042 288 182 9 34 310 69 336 1.41e-18 88.2
MS.gene32938.t1 MTR_3g450710 23.760 383 227 14 28 375 59 411 7.11e-18 85.9
MS.gene32938.t1 MTR_7g114960 24.460 417 278 11 21 422 50 444 1.03e-17 85.5
MS.gene32938.t1 MTR_1g040175 25.212 353 224 15 34 375 64 387 2.37e-17 84.3
MS.gene32938.t1 MTR_6g034485 34.677 124 81 0 180 303 2 125 2.44e-17 78.2
MS.gene32938.t1 MTR_3g112180 24.932 365 222 10 31 375 58 390 2.50e-17 84.3
MS.gene32938.t1 MTR_3g465930 27.018 285 184 7 34 310 69 337 5.30e-17 83.2
MS.gene32938.t1 MTR_7g114470 23.636 440 277 18 8 422 38 443 8.37e-17 82.8
MS.gene32938.t1 MTR_1g040215 25.936 374 233 17 34 395 64 405 1.59e-16 81.6
MS.gene32938.t1 MTR_6g015815 23.251 443 266 14 11 417 50 454 7.71e-16 79.7
MS.gene32938.t1 MTR_7g084940 25.496 353 224 12 32 373 67 391 1.21e-15 79.0
MS.gene32938.t1 MTR_7g014330 26.425 386 227 16 20 373 48 408 3.07e-15 77.8
MS.gene32938.t1 MTR_8g079970 24.819 415 256 19 31 427 56 432 8.08e-15 76.6
MS.gene32938.t1 MTR_3g049140 26.190 210 134 5 209 401 18 223 1.35e-14 73.9
MS.gene32938.t1 MTR_2g089765 23.272 434 259 16 20 407 61 466 1.58e-14 75.9
MS.gene32938.t1 MTR_1g040315 26.578 301 173 13 8 292 36 304 2.08e-14 75.1
MS.gene32938.t1 MTR_3g465910 27.465 284 180 9 35 310 69 334 2.80e-14 75.1
MS.gene32938.t1 MTR_2g015430 24.342 304 204 10 37 333 61 345 3.79e-14 74.3
MS.gene32938.t1 MTR_3g465880 26.280 293 177 9 35 310 69 339 4.99e-14 74.3
MS.gene32938.t1 MTR_3g028210 23.445 418 266 15 20 405 53 448 5.03e-14 74.3
MS.gene32938.t1 MTR_6g015320 23.990 396 240 14 7 373 42 405 6.36e-13 70.9
MS.gene32938.t1 MTR_7g056087 23.077 338 219 13 20 338 45 360 7.06e-13 70.5
MS.gene32938.t1 MTR_7g026830 51.852 54 26 0 100 153 44 97 1.11e-12 64.3
MS.gene32938.t1 MTR_6g015850 22.917 432 261 14 21 417 57 451 1.18e-12 69.7
MS.gene32938.t1 MTR_2g045540 31.959 97 58 1 209 305 18 106 3.75e-12 63.2
MS.gene32938.t1 MTR_2g089670 22.308 390 238 13 20 373 68 428 3.78e-12 68.6
MS.gene32938.t1 MTR_7g013850 22.838 451 272 20 5 405 40 464 5.54e-12 67.8
MS.gene32938.t1 MTR_6g015315 23.040 421 262 16 26 415 55 444 1.18e-11 66.6
MS.gene32938.t1 MTR_7g084910 25.735 272 169 10 32 291 68 318 1.87e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32938.t1 AT5G42830 54.312 429 179 5 1 427 37 450 1.59e-163 468
MS.gene32938.t1 AT5G07850 51.991 427 193 7 1 426 38 453 1.93e-153 442
MS.gene32938.t1 AT5G07870 49.083 436 200 6 1 426 38 461 2.14e-138 405
MS.gene32938.t1 AT5G07860 49.292 424 199 6 3 425 40 448 3.18e-136 399
MS.gene32938.t1 AT2G39980 37.474 475 218 12 1 426 36 480 8.45e-94 291
MS.gene32938.t1 AT5G67150 36.300 427 251 8 5 428 40 448 4.61e-88 275
MS.gene32938.t1 AT5G01210 38.593 469 220 12 1 430 34 473 3.62e-86 271
MS.gene32938.t1 AT3G50270 33.793 435 249 11 6 427 41 449 2.04e-74 240
MS.gene32938.t1 AT3G50280 33.333 423 254 10 6 422 39 439 1.17e-72 235
MS.gene32938.t1 AT3G50300 32.952 437 249 13 4 422 37 447 1.42e-68 224
MS.gene32938.t1 AT5G67160 34.018 438 230 14 6 428 41 434 2.14e-68 224
MS.gene32938.t1 AT5G38130 34.307 411 238 14 4 407 57 442 4.64e-66 218
MS.gene32938.t1 AT3G50280 35.714 308 187 5 120 422 8 309 5.78e-59 196
MS.gene32938.t1 AT5G23940 30.324 432 272 8 3 422 34 448 5.91e-54 187
MS.gene32938.t1 AT3G50290 39.847 261 142 6 121 372 15 269 1.77e-53 180
MS.gene32938.t1 AT5G48930 29.612 412 247 14 20 416 44 427 3.03e-38 144
MS.gene32938.t1 AT3G48720 32.716 324 182 12 7 321 40 336 1.34e-32 128
MS.gene32938.t1 AT5G41040 28.421 380 225 18 28 392 79 426 3.19e-32 127
MS.gene32938.t1 AT5G41040 28.421 380 225 18 28 392 63 410 3.80e-32 127
MS.gene32938.t1 AT5G63560 30.028 353 210 14 32 376 59 382 6.30e-30 120
MS.gene32938.t1 AT2G19070 27.962 422 250 19 24 416 48 444 2.39e-29 119
MS.gene32938.t1 AT2G40230 26.650 394 246 16 4 385 35 397 5.68e-22 98.2
MS.gene32938.t1 AT5G57840 27.252 433 242 19 11 413 39 428 1.37e-21 97.1
MS.gene32938.t1 AT1G78990 26.016 369 211 14 32 375 65 396 1.39e-20 94.0
MS.gene32938.t1 AT1G28680 25.987 304 198 11 37 333 65 348 4.54e-20 92.4
MS.gene32938.t1 AT1G28680 26.316 304 197 12 37 333 65 348 5.42e-20 91.7
MS.gene32938.t1 AT1G32910 24.557 395 231 17 7 375 46 399 8.33e-19 89.0
MS.gene32938.t1 AT1G65450 28.716 296 174 12 32 311 70 344 1.86e-18 87.8
MS.gene32938.t1 AT1G27620 25.989 354 223 12 34 375 58 384 5.77e-18 86.3
MS.gene32938.t1 AT3G26040 24.155 414 258 13 31 427 56 430 1.04e-17 85.5
MS.gene32938.t1 AT1G03390 25.182 413 262 15 19 412 64 448 3.66e-17 84.0
MS.gene32938.t1 AT5G07080 24.662 296 195 11 19 305 55 331 4.19e-17 83.6
MS.gene32938.t1 AT2G25150 25.000 320 209 9 27 335 60 359 1.55e-16 82.0
MS.gene32938.t1 AT3G29635 24.462 372 236 14 24 373 57 405 8.80e-15 76.6
MS.gene32938.t1 AT1G65450 29.412 187 110 6 140 311 1 180 6.37e-12 66.2

Find 86 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATTTCTATCCTCTTTC+TGG 0.061838 3.1:-80931267 MS.gene32938:CDS
ATTTGTATTGGATTCTTTAT+TGG 0.177909 3.1:+80930696 None:intergenic
AATATGTATGGCAATGAATT+TGG 0.188581 3.1:-80930307 MS.gene32938:CDS
CCTCTCCCACAAGAGTATTT+TGG 0.230083 3.1:-80930547 MS.gene32938:CDS
TTTGGAATTTCTTCAATATA+TGG 0.257098 3.1:-80930933 MS.gene32938:CDS
GAATTGCTTGAGAATGATTT+AGG 0.262759 3.1:-80930484 MS.gene32938:CDS
ATCTTCCTTCCAATCTTTAT+CGG 0.278893 3.1:-80930668 MS.gene32938:CDS
CGTGCCATTGCTATCCATTC+AGG 0.335903 3.1:-80931025 MS.gene32938:CDS
AATCTTTATCGGCATTTGTT+TGG 0.337842 3.1:-80930657 MS.gene32938:CDS
GGAAGATATTCCATTTGTAT+TGG 0.356628 3.1:+80930684 None:intergenic
CATAATCGGTGGTTCCTAGA+AGG 0.366417 3.1:-80930850 MS.gene32938:CDS
TTAGTGGACCGTAACCTTCT+AGG 0.370679 3.1:+80930836 None:intergenic
AGGAGGAGGAAGCATGGATT+TGG 0.380210 3.1:-80930206 MS.gene32938:CDS
AAACAAATGCCGATAAAGAT+TGG 0.404666 3.1:+80930659 None:intergenic
CCACACTGAAGGGATCAAAA+TGG 0.413713 3.1:+80930353 None:intergenic
TCTATGCACTACATCCAAAA+AGG 0.416467 3.1:-80931387 MS.gene32938:CDS
GTTTATCGCTAACTTGCAAC+TGG 0.422888 3.1:+80930591 None:intergenic
TGGTTATTTGGTGAAGTTGT+TGG 0.431090 3.1:+80931348 None:intergenic
ATTTGAAGGAGGAGGAAGCA+TGG 0.434087 3.1:-80930212 MS.gene32938:CDS
AAAGAGGATAGAAATAGAAA+AGG 0.445181 3.1:+80931271 None:intergenic
TTTAGGTTGGGCAGCATTGA+AGG 0.446052 3.1:-80930467 MS.gene32938:CDS
AATAAAGAATCCAATACAAA+TGG 0.447104 3.1:-80930694 MS.gene32938:CDS
ATCACCAATACAGTGGTTCA+TGG 0.449644 3.1:+80930966 None:intergenic
AAATAATTTCTCTCTCAACT+TGG 0.456187 3.1:+80930759 None:intergenic
GGATACGCAAACAAATTTGA+TGG 0.459085 3.1:-80930256 MS.gene32938:CDS
TGCTTGAGAATGATTTAGGT+TGG 0.465724 3.1:-80930480 MS.gene32938:CDS
CCAAAATACTCTTGTGGGAG+AGG 0.467713 3.1:+80930547 None:intergenic
GCTTGAGAATGATTTAGGTT+GGG 0.480817 3.1:-80930479 MS.gene32938:CDS
TCTAGCGCACTCATTGCATT+AGG 0.481834 3.1:+80930169 None:intergenic
TCCTCCTTCAAATCCCTCAT+AGG 0.483907 3.1:+80930222 None:intergenic
GGTTGATGCTCCACCTATCA+TGG 0.487108 3.1:-80931094 MS.gene32938:CDS
TATCGATAAAGCAATCAATC+AGG 0.488699 3.1:-80931127 MS.gene32938:CDS
TCACCGAGGTTCAATATGTA+TGG 0.489006 3.1:-80930319 MS.gene32938:CDS
CGACCAAGTTGGTAGACAAC+CGG 0.489315 3.1:+80930376 None:intergenic
AAGAGGATAGAAATAGAAAA+GGG 0.491247 3.1:+80931272 None:intergenic
TGGCAACGTGACAGCCTATG+AGG 0.496952 3.1:-80930236 MS.gene32938:CDS
TCAAACAATGAGTCCATGAT+AGG 0.499726 3.1:+80931081 None:intergenic
ATGATAGGTGGAGCATCAAC+CGG 0.505702 3.1:+80931096 None:intergenic
AGGAACCACCGATTATGAAA+AGG 0.505726 3.1:+80930856 None:intergenic
AGTTTCCAAAATACTCTTGT+GGG 0.512647 3.1:+80930542 None:intergenic
CGGTGGTTCCTAGAAGGTTA+CGG 0.519415 3.1:-80930844 MS.gene32938:CDS
TATGGCAATGAATTTGGAAT+GGG 0.522099 3.1:-80930301 MS.gene32938:CDS
AATAGAAAAGGGCATGAGAG+AGG 0.530285 3.1:+80931283 None:intergenic
ATAGAAAAGGGCATGAGAGA+GGG 0.532663 3.1:+80931284 None:intergenic
TTGAACGGAAGATCAATTAG+TGG 0.535580 3.1:+80930820 None:intergenic
ACAGCCTATGAGGGATTTGA+AGG 0.535914 3.1:-80930226 MS.gene32938:CDS
AATAATTTCTCTCTCAACTT+GGG 0.539322 3.1:+80930760 None:intergenic
CACAAGCTCAGTGACCTGAA+TGG 0.539507 3.1:+80931011 None:intergenic
CATTCAGGTCACTGAGCTTG+TGG 0.541263 3.1:-80931010 MS.gene32938:CDS
AGGAGGAAGCATGGATTTGG+CGG 0.541497 3.1:-80930203 MS.gene32938:CDS
TGCATAGATGAATCTAGCTC+CGG 0.545101 3.1:+80931173 None:intergenic
TGTTCCATGAACCACTGTAT+TGG 0.553473 3.1:-80930970 MS.gene32938:CDS
AACTCGTCAAAATGTTTGAA+CGG 0.562459 3.1:+80930805 None:intergenic
TATGAGGGATTTGAAGGAGG+AGG 0.567796 3.1:-80930220 MS.gene32938:CDS
AAATGCCGATAAAGATTGGA+AGG 0.570447 3.1:+80930663 None:intergenic
GTGACAAGACGGCCAGAAAG+AGG 0.571143 3.1:+80931255 None:intergenic
GATCAAAATGGCGACCAAGT+TGG 0.573234 3.1:+80930365 None:intergenic
GTATGGCAATGAATTTGGAA+TGG 0.573460 3.1:-80930302 MS.gene32938:CDS
TCTCCGGTTGTCTACCAACT+TGG 0.573892 3.1:-80930379 MS.gene32938:CDS
CAGGTCACTGAGCTTGTGGA+TGG 0.574257 3.1:-80931006 MS.gene32938:CDS
AAATACTCTTGTGGGAGAGG+TGG 0.583943 3.1:+80930550 None:intergenic
GAGTTTCCAAAATACTCTTG+TGG 0.591607 3.1:+80930541 None:intergenic
ATGAACCACTGTATTGGTGA+TGG 0.594810 3.1:-80930964 MS.gene32938:CDS
CTTGACATCACCACACTGAA+GGG 0.595257 3.1:+80930343 None:intergenic
GATAGAGCAATCAATCATGA+TGG 0.595544 3.1:-80931054 MS.gene32938:CDS
ATATATCAAGCTCAAGCGAA+AGG 0.596890 3.1:-80930904 MS.gene32938:CDS
AGCTAGATTCATCTATGCAA+CGG 0.600026 3.1:-80931169 MS.gene32938:CDS
AACAATGAGTCCATGATAGG+TGG 0.601529 3.1:+80931084 None:intergenic
TGAACCACTGTATTGGTGAT+GGG 0.605754 3.1:-80930963 MS.gene32938:CDS
ACTTGACATCACCACACTGA+AGG 0.607473 3.1:+80930342 None:intergenic
AATCAATCAGGATGATCCAC+CGG 0.617323 3.1:-80931115 MS.gene32938:CDS
GTAAGTACAGAAGCGACTGT+AGG 0.620746 3.1:-80930508 MS.gene32938:CDS
GTGACCTGAATGGATAGCAA+TGG 0.627382 3.1:+80931021 None:intergenic
CTGAATGGATAGCAATGGCA+CGG 0.630426 3.1:+80931026 None:intergenic
GGCAACGTGACAGCCTATGA+GGG 0.632849 3.1:-80930235 MS.gene32938:CDS
TTGCCATACATATTGAACCT+CGG 0.635766 3.1:+80930316 None:intergenic
GCCTATGAGGGATTTGAAGG+AGG 0.640344 3.1:-80930223 MS.gene32938:CDS
TATTGCAAAACTGAAAGCAA+AGG 0.644608 3.1:-80930719 MS.gene32938:CDS
ACCACCGATTATGAAAAGGT+TGG 0.648796 3.1:+80930860 None:intergenic
TGTGAATCATGATGATAGAG+AGG 0.662674 3.1:-80930431 MS.gene32938:CDS
ATAGGTGGAGCATCAACCGG+TGG 0.689108 3.1:+80931099 None:intergenic
GATTGCAGTAATGATAACAC+CGG 0.699640 3.1:-80931192 MS.gene32938:CDS
AAGTCCCATCACCAATACAG+TGG 0.718052 3.1:+80930959 None:intergenic
GAGTGCGCTAGAATCAGACG+AGG 0.735827 3.1:-80930158 MS.gene32938:CDS
TAGATTCATCTATGCAACGG+TGG 0.776095 3.1:-80931166 MS.gene32938:CDS
TGGTGATGTCAAGTTCACCG+AGG 0.799442 3.1:-80930333 MS.gene32938:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATCCAATACAAA+TGG - chr3.1:80930810-80930829 MS.gene32938:CDS 20.0%
!! ATTTGTATTGGATTCTTTAT+TGG + chr3.1:80930811-80930830 None:intergenic 20.0%
!!! ATGAAATTTTGTTGGTTATT+TGG + chr3.1:80930171-80930190 None:intergenic 20.0%
!!! GATTATCAATGAAATTTTGT+TGG + chr3.1:80930179-80930198 None:intergenic 20.0%
!!! TTGTTGGTTTTTTGAATAAT+AGG + chr3.1:80930143-80930162 None:intergenic 20.0%
!!! TTTGGAATTTCTTCAATATA+TGG - chr3.1:80930571-80930590 MS.gene32938:CDS 20.0%
! AAAGAGGATAGAAATAGAAA+AGG + chr3.1:80930236-80930255 None:intergenic 25.0%
! AAATAATTTCTCTCTCAACT+TGG + chr3.1:80930748-80930767 None:intergenic 25.0%
! AAGAGGATAGAAATAGAAAA+GGG + chr3.1:80930235-80930254 None:intergenic 25.0%
! AATAATTTCTCTCTCAACTT+GGG + chr3.1:80930747-80930766 None:intergenic 25.0%
! AATATGTATGGCAATGAATT+TGG - chr3.1:80931197-80931216 MS.gene32938:CDS 25.0%
! ATTACTGCAATCAACAAAAA+TGG + chr3.1:80930304-80930323 None:intergenic 25.0%
!! TTTTGAATAATAGGCCTTTT+TGG + chr3.1:80930134-80930153 None:intergenic 25.0%
AAACAAATGCCGATAAAGAT+TGG + chr3.1:80930848-80930867 None:intergenic 30.0%
AACTCGTCAAAATGTTTGAA+CGG + chr3.1:80930702-80930721 None:intergenic 30.0%
AATCTTTATCGGCATTTGTT+TGG - chr3.1:80930847-80930866 MS.gene32938:CDS 30.0%
ACAAAAATGGTATAAGAGTG+AGG + chr3.1:80930291-80930310 None:intergenic 30.0%
AGTTTCCAAAATACTCTTGT+GGG + chr3.1:80930965-80930984 None:intergenic 30.0%
ATCTTCCTTCCAATCTTTAT+CGG - chr3.1:80930836-80930855 MS.gene32938:CDS 30.0%
GAATTGCTTGAGAATGATTT+AGG - chr3.1:80931020-80931039 MS.gene32938:CDS 30.0%
GGAAGATATTCCATTTGTAT+TGG + chr3.1:80930823-80930842 None:intergenic 30.0%
TATCGATAAAGCAATCAATC+AGG - chr3.1:80930377-80930396 MS.gene32938:CDS 30.0%
TATTGCAAAACTGAAAGCAA+AGG - chr3.1:80930785-80930804 MS.gene32938:CDS 30.0%
! TATGGCAATGAATTTGGAAT+GGG - chr3.1:80931203-80931222 MS.gene32938:CDS 30.0%
! TTTTATGAATGCAGTTTCTG+TGG - chr3.1:80931370-80931389 MS.gene32938:CDS 30.0%
!! TTCTATTTCTATCCTCTTTC+TGG - chr3.1:80930237-80930256 MS.gene32938:CDS 30.0%
AAATGCCGATAAAGATTGGA+AGG + chr3.1:80930844-80930863 None:intergenic 35.0%
AGCTAGATTCATCTATGCAA+CGG - chr3.1:80930335-80930354 MS.gene32938:CDS 35.0%
ATATATCAAGCTCAAGCGAA+AGG - chr3.1:80930600-80930619 MS.gene32938:CDS 35.0%
CAAAAATTGGTTAGAGTCTC+CGG - chr3.1:80931109-80931128 MS.gene32938:CDS 35.0%
GAGTTTCCAAAATACTCTTG+TGG + chr3.1:80930966-80930985 None:intergenic 35.0%
GATAGAGCAATCAATCATGA+TGG - chr3.1:80930450-80930469 MS.gene32938:CDS 35.0%
GATTGCAGTAATGATAACAC+CGG - chr3.1:80930312-80930331 MS.gene32938:CDS 35.0%
GGATACGCAAACAAATTTGA+TGG - chr3.1:80931248-80931267 MS.gene32938:CDS 35.0%
GTATGGCAATGAATTTGGAA+TGG - chr3.1:80931202-80931221 MS.gene32938:CDS 35.0%
TCAAACAATGAGTCCATGAT+AGG + chr3.1:80930426-80930445 None:intergenic 35.0%
TCTATGCACTACATCCAAAA+AGG - chr3.1:80930117-80930136 MS.gene32938:CDS 35.0%
TGAGAGAGATGCTCAAAAAT+TGG - chr3.1:80931096-80931115 MS.gene32938:CDS 35.0%
TGCTTGAGAATGATTTAGGT+TGG - chr3.1:80931024-80931043 MS.gene32938:CDS 35.0%
TGTGAATCATGATGATAGAG+AGG - chr3.1:80931073-80931092 MS.gene32938:CDS 35.0%
TTGAACGGAAGATCAATTAG+TGG + chr3.1:80930687-80930706 None:intergenic 35.0%
TTGCCATACATATTGAACCT+CGG + chr3.1:80931191-80931210 None:intergenic 35.0%
! AAAGCAGTTGCGATTTTAAG+TGG - chr3.1:80931227-80931246 MS.gene32938:CDS 35.0%
! GCTTGAGAATGATTTAGGTT+GGG - chr3.1:80931025-80931044 MS.gene32938:CDS 35.0%
! TGGTTATTTGGTGAAGTTGT+TGG + chr3.1:80930159-80930178 None:intergenic 35.0%
AACAATGAGTCCATGATAGG+TGG + chr3.1:80930423-80930442 None:intergenic 40.0%
AATCAATCAGGATGATCCAC+CGG - chr3.1:80930389-80930408 MS.gene32938:CDS 40.0%
ATCACCAATACAGTGGTTCA+TGG + chr3.1:80930541-80930560 None:intergenic 40.0%
TAGATTCATCTATGCAACGG+TGG - chr3.1:80930338-80930357 MS.gene32938:CDS 40.0%
TCACCGAGGTTCAATATGTA+TGG - chr3.1:80931185-80931204 MS.gene32938:CDS 40.0%
TGTTCCATGAACCACTGTAT+TGG - chr3.1:80930534-80930553 MS.gene32938:CDS 40.0%
! AATAGAAAAGGGCATGAGAG+AGG + chr3.1:80930224-80930243 None:intergenic 40.0%
! ACCAACCTTTTCATAATCGG+TGG - chr3.1:80930643-80930662 MS.gene32938:CDS 40.0%
! ACCACCGATTATGAAAAGGT+TGG + chr3.1:80930647-80930666 None:intergenic 40.0%
! AGGAACCACCGATTATGAAA+AGG + chr3.1:80930651-80930670 None:intergenic 40.0%
! AGTGAGGATCTTGAGTTTTG+TGG + chr3.1:80930275-80930294 None:intergenic 40.0%
! ATAGAAAAGGGCATGAGAGA+GGG + chr3.1:80930223-80930242 None:intergenic 40.0%
! ATGAACCACTGTATTGGTGA+TGG - chr3.1:80930540-80930559 MS.gene32938:CDS 40.0%
! CGCACCAACCTTTTCATAAT+CGG - chr3.1:80930640-80930659 MS.gene32938:CDS 40.0%
! CTTGTGGATGGTGTTTTCAT+AGG - chr3.1:80930510-80930529 MS.gene32938:CDS 40.0%
! GTTTATCGCTAACTTGCAAC+TGG + chr3.1:80930916-80930935 None:intergenic 40.0%
! TGAACCACTGTATTGGTGAT+GGG - chr3.1:80930541-80930560 MS.gene32938:CDS 40.0%
! TGCATAGATGAATCTAGCTC+CGG + chr3.1:80930334-80930353 None:intergenic 40.0%
!! GTGAGGATCTTGAGTTTTGT+GGG + chr3.1:80930274-80930293 None:intergenic 40.0%
!! TTTTGGAAACTCAGTTGACG+TGG - chr3.1:80930974-80930993 MS.gene32938:CDS 40.0%
!!! TTGGTGATGGGACTTCTTTT+TGG - chr3.1:80930553-80930572 MS.gene32938:CDS 40.0%
AAATACTCTTGTGGGAGAGG+TGG + chr3.1:80930957-80930976 None:intergenic 45.0%
AAGTCCCATCACCAATACAG+TGG + chr3.1:80930548-80930567 None:intergenic 45.0%
ACAGCCTATGAGGGATTTGA+AGG - chr3.1:80931278-80931297 MS.gene32938:CDS 45.0%
ACTTGACATCACCACACTGA+AGG + chr3.1:80931165-80931184 None:intergenic 45.0%
ATTTGAAGGAGGAGGAAGCA+TGG - chr3.1:80931292-80931311 MS.gene32938:CDS 45.0%
CATAATCGGTGGTTCCTAGA+AGG - chr3.1:80930654-80930673 MS.gene32938:CDS 45.0%
CCAAAATACTCTTGTGGGAG+AGG + chr3.1:80930960-80930979 None:intergenic 45.0%
CCACACTGAAGGGATCAAAA+TGG + chr3.1:80931154-80931173 None:intergenic 45.0%
CTGAATGGATAGCAATGGCA+CGG + chr3.1:80930481-80930500 None:intergenic 45.0%
CTTGACATCACCACACTGAA+GGG + chr3.1:80931164-80931183 None:intergenic 45.0%
GATCAAAATGGCGACCAAGT+TGG + chr3.1:80931142-80931161 None:intergenic 45.0%
GTAAGTACAGAAGCGACTGT+AGG - chr3.1:80930996-80931015 MS.gene32938:CDS 45.0%
GTGACCTGAATGGATAGCAA+TGG + chr3.1:80930486-80930505 None:intergenic 45.0%
TATGAGGGATTTGAAGGAGG+AGG - chr3.1:80931284-80931303 MS.gene32938:CDS 45.0%
TCCTCCTTCAAATCCCTCAT+AGG + chr3.1:80931285-80931304 None:intergenic 45.0%
TCTAGCGCACTCATTGCATT+AGG + chr3.1:80931338-80931357 None:intergenic 45.0%
! ATGATAGGTGGAGCATCAAC+CGG + chr3.1:80930411-80930430 None:intergenic 45.0%
! TTAGTGGACCGTAACCTTCT+AGG + chr3.1:80930671-80930690 None:intergenic 45.0%
!! CCATTTTGATCCCTTCAGTG+TGG - chr3.1:80931151-80931170 MS.gene32938:CDS 45.0%
!! GAGTTTTGTGGGTGACAAGA+CGG + chr3.1:80930263-80930282 None:intergenic 45.0%
!! TTTAGGTTGGGCAGCATTGA+AGG - chr3.1:80931037-80931056 MS.gene32938:CDS 45.0%
!!! CCTCTCCCACAAGAGTATTT+TGG - chr3.1:80930957-80930976 MS.gene32938:CDS 45.0%
CACAAGCTCAGTGACCTGAA+TGG + chr3.1:80930496-80930515 None:intergenic 50.0%
CATTCAGGTCACTGAGCTTG+TGG - chr3.1:80930494-80930513 MS.gene32938:CDS 50.0%
CGGTGGTTCCTAGAAGGTTA+CGG - chr3.1:80930660-80930679 MS.gene32938:CDS 50.0%
GCCTATGAGGGATTTGAAGG+AGG - chr3.1:80931281-80931300 MS.gene32938:CDS 50.0%
TCTCCGGTTGTCTACCAACT+TGG - chr3.1:80931125-80931144 MS.gene32938:CDS 50.0%
! AGGAGGAAGCATGGATTTGG+CGG - chr3.1:80931301-80931320 MS.gene32938:CDS 50.0%
! AGGAGGAGGAAGCATGGATT+TGG - chr3.1:80931298-80931317 MS.gene32938:CDS 50.0%
! CGTGCCATTGCTATCCATTC+AGG - chr3.1:80930479-80930498 MS.gene32938:CDS 50.0%
! GGTTGATGCTCCACCTATCA+TGG - chr3.1:80930410-80930429 MS.gene32938:CDS 50.0%
! TGGTGATGTCAAGTTCACCG+AGG - chr3.1:80931171-80931190 MS.gene32938:CDS 50.0%
!! CGACCAAGTTGGTAGACAAC+CGG + chr3.1:80931131-80931150 None:intergenic 50.0%
CAGGTCACTGAGCTTGTGGA+TGG - chr3.1:80930498-80930517 MS.gene32938:CDS 55.0%
GAGTGCGCTAGAATCAGACG+AGG - chr3.1:80931346-80931365 MS.gene32938:CDS 55.0%
GGCAACGTGACAGCCTATGA+GGG - chr3.1:80931269-80931288 MS.gene32938:CDS 55.0%
GTGACAAGACGGCCAGAAAG+AGG + chr3.1:80930252-80930271 None:intergenic 55.0%
TGGCAACGTGACAGCCTATG+AGG - chr3.1:80931268-80931287 MS.gene32938:CDS 55.0%
! ATAGGTGGAGCATCAACCGG+TGG + chr3.1:80930408-80930427 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 80930111 80931415 80930111 ID=MS.gene32938
chr3.1 mRNA 80930111 80931415 80930111 ID=MS.gene32938.t1;Parent=MS.gene32938
chr3.1 exon 80930111 80931415 80930111 ID=MS.gene32938.t1.exon1;Parent=MS.gene32938.t1
chr3.1 CDS 80930111 80931415 80930111 ID=cds.MS.gene32938.t1;Parent=MS.gene32938.t1
Gene Sequence

>MS.gene32938

ATGTCATCTATGCACTACATCCAAAAAGGCCTATTATTCAAAAAACCAACAACTTCACCAAATAACCAACAAAATTTCATTGATAATCTGTTAGAGAAGCTCAAACACTCCCTCTCTCATGCCCTTTTCTATTTCTATCCTCTTTCTGGCCGTCTTGTCACCCACAAAACTCAAGATCCTCACTCTTATACCATTTTTGTTGATTGCAGTAATGATAACACCGGAGCTAGATTCATCTATGCAACGGTGGATGCAACAATATATGATATCGATAAAGCAATCAATCAGGATGATCCACCGGTTGATGCTCCACCTATCATGGACTCATTGTTTGATCTCGATAGAGCAATCAATCATGATGGTCATGCCGTGCCATTGCTATCCATTCAGGTCACTGAGCTTGTGGATGGTGTTTTCATAGGCTGTTCCATGAACCACTGTATTGGTGATGGGACTTCTTTTTGGAATTTCTTCAATATATGGTCTGAAATATATCAAGCTCAAGCGAAAGGTGATGATGTTCTCATTTCGCACCAACCTTTTCATAATCGGTGGTTCCTAGAAGGTTACGGTCCACTAATTGATCTTCCGTTCAAACATTTTGACGAGTTTACAAGCAGATTTGAAGCAGTACCCAAGTTGAGAGAGAAATTATTTCACTTTAGTGCAGAGTCTATTGCAAAACTGAAAGCAAAGGCCAATAAAGAATCCAATACAAATGGAATATCTTCCTTCCAATCTTTATCGGCATTTGTTTGGAGAAGTGTCGCACGTGCACGAAGACTAAAACATGATCAAATAACCAGTTGCAAGTTAGCGATAAACAATAGATCAAGAATCAAACCACCTCTCCCACAAGAGTATTTTGGAAACTCAGTTGACGTGGTAAGTACAGAAGCGACTGTAGGCGAATTGCTTGAGAATGATTTAGGTTGGGCAGCATTGAAGGTACATACACTTGTTGTGAATCATGATGATAGAGAGGTGAGAGAGATGCTCAAAAATTGGTTAGAGTCTCCGGTTGTCTACCAACTTGGTCGCCATTTTGATCCCTTCAGTGTGGTGATGTCAAGTTCACCGAGGTTCAATATGTATGGCAATGAATTTGGAATGGGAAAAGCAGTTGCGATTTTAAGTGGATACGCAAACAAATTTGATGGCAACGTGACAGCCTATGAGGGATTTGAAGGAGGAGGAAGCATGGATTTGGCGGTGTCTCTTTTGCCTAATGCAATGAGTGCGCTAGAATCAGACGAGGATTTTATGAATGCAGTTTCTGTGGTCAATTTTTCTAATATTCTGTGA
Protein sequence

>MS.gene32938.t1

MSSMHYIQKGLLFKKPTTSPNNQQNFIDNLLEKLKHSLSHALFYFYPLSGRLVTHKTQDPHSYTIFVDCSNDNTGARFIYATVDATIYDIDKAINQDDPPVDAPPIMDSLFDLDRAINHDGHAVPLLSIQVTELVDGVFIGCSMNHCIGDGTSFWNFFNIWSEIYQAQAKGDDVLISHQPFHNRWFLEGYGPLIDLPFKHFDEFTSRFEAVPKLREKLFHFSAESIAKLKAKANKESNTNGISSFQSLSAFVWRSVARARRLKHDQITSCKLAINNRSRIKPPLPQEYFGNSVDVVSTEATVGELLENDLGWAALKVHTLVVNHDDREVREMLKNWLESPVVYQLGRHFDPFSVVMSSSPRFNMYGNEFGMGKAVAILSGYANKFDGNVTAYEGFEGGGSMDLAVSLLPNAMSALESDEDFMNAVSVVNFSNIL