AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38700.t1 MTR_3g101960 91.236 445 39 0 1 445 1 445 0.0 863
MS.gene38700.t1 MTR_3g102020 84.245 457 71 1 1 456 1 457 0.0 815
MS.gene38700.t1 MTR_3g101970 85.841 452 49 2 1 451 1 438 0.0 809
MS.gene38700.t1 MTR_3g102010 79.783 460 85 2 1 456 2 457 0.0 767
MS.gene38700.t1 MTR_3g101900 69.011 455 130 4 1 451 2 449 0.0 630
MS.gene38700.t1 MTR_3g101930 66.889 450 135 5 9 449 10 454 0.0 613
MS.gene38700.t1 MTR_3g102030 74.528 318 55 2 86 403 29 320 3.21e-173 488
MS.gene38700.t1 MTR_4g015180 41.833 502 221 11 5 452 5 489 6.41e-107 326
MS.gene38700.t1 MTR_3g070170 39.300 486 221 12 5 446 11 466 9.41e-102 312
MS.gene38700.t1 MTR_5g081960 40.040 497 224 13 5 446 5 482 3.07e-101 311
MS.gene38700.t1 MTR_4g094842 37.773 458 257 11 3 446 1 444 7.10e-90 280
MS.gene38700.t1 MTR_8g041770 39.059 425 243 9 29 450 30 441 3.54e-89 278
MS.gene38700.t1 MTR_4g022800 38.149 443 245 11 21 450 16 442 7.81e-89 278
MS.gene38700.t1 MTR_4g022720 39.130 437 242 11 21 450 16 435 3.05e-84 266
MS.gene38700.t1 MTR_0190s0020 37.850 428 248 10 28 450 28 442 1.48e-83 264
MS.gene38700.t1 MTR_4g094835 37.529 429 247 8 25 446 28 442 1.59e-83 264
MS.gene38700.t1 MTR_4g022825 36.199 442 255 9 20 450 15 440 1.33e-81 259
MS.gene38700.t1 MTR_4g094838 37.296 429 230 9 29 446 28 428 2.64e-81 258
MS.gene38700.t1 MTR_4g022670 36.054 441 256 9 21 450 16 441 9.37e-81 257
MS.gene38700.t1 MTR_3g088420 37.011 435 248 10 23 450 18 433 1.96e-79 253
MS.gene38700.t1 MTR_7g026860 35.973 442 252 11 21 450 16 438 2.74e-78 250
MS.gene38700.t1 MTR_4g094828 35.914 465 262 14 6 451 4 451 2.98e-78 251
MS.gene38700.t1 MTR_4g022290 35.238 420 240 10 23 429 24 424 1.53e-75 243
MS.gene38700.t1 MTR_3g026310 37.104 442 241 13 29 450 24 448 1.64e-75 243
MS.gene38700.t1 MTR_8g079710 35.811 444 239 16 21 445 21 437 4.51e-71 232
MS.gene38700.t1 MTR_4g094832 38.109 349 201 6 105 446 86 426 1.76e-68 224
MS.gene38700.t1 MTR_4g022760 34.961 389 218 10 21 386 16 392 2.51e-63 210
MS.gene38700.t1 MTR_6g045553 31.612 484 270 12 5 449 13 474 2.57e-62 209
MS.gene38700.t1 MTR_6g016815 30.357 448 227 14 5 450 17 381 2.48e-47 167
MS.gene38700.t1 MTR_8g040780 36.493 211 129 4 240 447 20 228 1.67e-39 143
MS.gene38700.t1 MTR_8g075610 28.030 396 234 13 68 440 47 414 1.05e-35 137
MS.gene38700.t1 MTR_2g064495 28.021 389 246 13 68 440 56 426 2.20e-34 133
MS.gene38700.t1 MTR_4g007540 26.652 454 285 17 6 440 3 427 2.98e-34 133
MS.gene38700.t1 MTR_2g105330 27.763 389 247 13 68 440 56 426 9.03e-34 132
MS.gene38700.t1 MTR_4g127650 26.667 450 254 17 19 431 30 440 4.21e-28 116
MS.gene38700.t1 MTR_7g026850 41.611 149 76 4 43 190 21 159 1.71e-27 108
MS.gene38700.t1 MTR_7g026770 37.853 177 89 3 155 331 132 287 3.67e-27 110
MS.gene38700.t1 MTR_4g097090 25.968 439 261 17 29 431 30 440 1.19e-26 112
MS.gene38700.t1 MTR_3g098980 28.477 302 191 10 68 358 66 353 1.02e-24 106
MS.gene38700.t1 MTR_8g040760 34.101 217 132 6 8 223 6 212 1.21e-24 101
MS.gene38700.t1 MTR_8g096530 27.070 314 200 9 62 362 53 350 2.23e-24 105
MS.gene38700.t1 MTR_3g049150 25.120 418 257 14 56 440 46 440 7.30e-24 103
MS.gene38700.t1 MTR_4g094825 36.667 150 84 2 301 450 253 391 1.00e-23 102
MS.gene38700.t1 MTR_4g094825 32.821 195 114 6 29 220 92 272 4.24e-21 95.1
MS.gene38700.t1 MTR_5g076700 24.945 457 289 18 4 436 5 431 2.11e-23 102
MS.gene38700.t1 MTR_3g026950 24.590 427 251 15 62 445 48 446 1.76e-22 99.8
MS.gene38700.t1 MTR_6g053810 30.293 307 188 13 53 344 54 349 4.57e-22 99.0
MS.gene38700.t1 MTR_3g112180 26.276 392 256 11 18 399 22 390 1.14e-21 97.4
MS.gene38700.t1 MTR_7g114550 26.258 457 261 21 30 447 24 443 1.58e-21 97.1
MS.gene38700.t1 MTR_7g114470 25.761 427 279 16 30 436 25 433 2.07e-21 96.7
MS.gene38700.t1 MTR_6g034495 41.525 118 65 3 79 193 45 161 2.78e-21 90.9
MS.gene38700.t1 MTR_7g114490 26.818 440 266 19 30 441 24 435 1.80e-20 94.4
MS.gene38700.t1 MTR_7g114520 26.964 471 262 21 30 456 25 457 2.57e-20 93.6
MS.gene38700.t1 MTR_7g114560 26.185 443 279 18 30 447 24 443 7.74e-20 92.0
MS.gene38700.t1 MTR_7g027175 27.619 315 202 10 56 358 56 356 1.03e-19 91.7
MS.gene38700.t1 MTR_7g114960 25.310 403 262 12 65 446 60 444 1.37e-19 91.3
MS.gene38700.t1 MTR_7g086000 27.114 402 233 18 71 433 63 443 6.31e-19 89.4
MS.gene38700.t1 MTR_1g081660 26.515 396 252 15 65 443 70 443 9.67e-19 88.6
MS.gene38700.t1 MTR_6g034485 36.290 124 78 1 205 327 2 125 1.12e-18 82.4
MS.gene38700.t1 MTR_1g040175 26.757 370 228 17 65 419 61 402 1.38e-18 88.2
MS.gene38700.t1 MTR_1g040195 25.916 382 251 15 48 418 16 376 1.84e-18 87.8
MS.gene38700.t1 MTR_3g465980 26.901 342 206 14 13 334 17 334 8.05e-18 85.9
MS.gene38700.t1 MTR_3g071320 25.060 415 256 18 5 395 4 387 1.19e-17 85.1
MS.gene38700.t1 MTR_4g055170 25.267 281 183 8 68 334 69 336 1.32e-16 82.4
MS.gene38700.t1 MTR_3g465990 26.822 343 205 15 13 334 17 334 3.34e-16 80.9
MS.gene38700.t1 MTR_7g084940 23.975 317 199 11 64 363 65 356 1.16e-14 76.3
MS.gene38700.t1 MTR_3g465930 26.821 302 183 11 50 331 51 334 2.47e-14 75.1
MS.gene38700.t1 MTR_7g026830 47.143 70 36 1 111 179 28 97 2.56e-13 66.2
MS.gene38700.t1 MTR_5g090830 23.824 319 199 14 48 342 49 347 3.59e-13 71.6
MS.gene38700.t1 MTR_2g015430 24.172 302 199 9 71 358 61 346 3.68e-13 71.6
MS.gene38700.t1 MTR_7g085990 25.749 334 199 16 57 363 44 355 4.19e-13 71.6
MS.gene38700.t1 MTR_7g085930 24.189 339 206 14 57 367 48 363 6.67e-13 70.9
MS.gene38700.t1 MTR_5g090810 23.511 319 200 14 48 342 49 347 7.05e-13 70.9
MS.gene38700.t1 MTR_3g465910 27.333 300 178 11 52 331 52 331 1.01e-12 70.5
MS.gene38700.t1 MTR_3g049140 24.242 198 136 4 242 425 27 224 1.62e-12 67.8
MS.gene38700.t1 MTR_3g465950 26.523 279 176 8 69 331 69 334 2.55e-12 68.9
MS.gene38700.t1 MTR_7g013850 22.673 419 266 13 30 402 30 436 4.85e-12 68.2
MS.gene38700.t1 MTR_7g014620 22.477 436 291 14 29 441 15 426 1.25e-11 66.6
MS.gene38700.t1 MTR_5g090880 22.703 370 234 16 64 399 63 414 2.89e-11 65.9
MS.gene38700.t1 MTR_6g015320 22.018 436 291 15 29 436 29 443 6.81e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38700.t1 AT5G42830 55.830 446 181 4 13 451 14 450 1.57e-179 509
MS.gene38700.t1 AT5G07850 53.363 446 207 1 6 450 8 453 2.13e-176 502
MS.gene38700.t1 AT5G07860 53.125 448 199 5 6 449 8 448 9.26e-164 470
MS.gene38700.t1 AT5G07870 50.655 458 209 3 6 450 8 461 9.65e-162 465
MS.gene38700.t1 AT2G39980 40.202 495 226 11 5 450 7 480 6.65e-105 320
MS.gene38700.t1 AT5G01210 40.535 486 232 11 5 452 5 471 1.21e-97 301
MS.gene38700.t1 AT5G67150 38.546 454 258 11 8 452 7 448 9.32e-97 298
MS.gene38700.t1 AT3G50270 35.526 456 272 11 6 451 6 449 1.43e-82 262
MS.gene38700.t1 AT3G50280 34.513 452 275 11 3 446 1 439 6.51e-77 247
MS.gene38700.t1 AT3G50300 33.871 434 268 8 6 432 4 425 5.19e-75 242
MS.gene38700.t1 AT5G38130 33.790 438 269 11 2 432 20 443 3.23e-71 233
MS.gene38700.t1 AT5G67160 34.000 450 270 12 8 452 7 434 8.89e-69 225
MS.gene38700.t1 AT5G23940 33.043 460 279 12 6 448 3 450 1.61e-63 213
MS.gene38700.t1 AT3G50280 37.217 309 183 6 144 446 6 309 6.03e-54 183
MS.gene38700.t1 AT3G50290 37.066 259 150 6 147 396 15 269 9.71e-43 152
MS.gene38700.t1 AT5G48930 28.009 457 275 17 6 440 3 427 4.38e-38 144
MS.gene38700.t1 AT2G19070 28.864 440 247 18 29 431 25 435 8.53e-32 127
MS.gene38700.t1 AT5G41040 28.896 308 197 8 62 358 63 359 2.33e-28 117
MS.gene38700.t1 AT5G41040 28.896 308 197 8 62 358 79 375 2.91e-28 117
MS.gene38700.t1 AT3G48720 27.512 418 249 19 18 420 18 396 7.31e-23 100
MS.gene38700.t1 AT5G63560 27.606 355 215 13 18 358 19 345 4.17e-22 98.6
MS.gene38700.t1 AT2G40230 27.200 375 214 13 63 409 54 397 4.41e-22 98.6
MS.gene38700.t1 AT5G57840 25.450 444 299 15 6 436 3 427 6.38e-21 95.1
MS.gene38700.t1 AT1G65450 27.815 302 179 12 56 335 60 344 9.87e-20 91.7
MS.gene38700.t1 AT1G31490 26.406 409 244 20 62 445 56 432 1.12e-17 85.5
MS.gene38700.t1 AT1G78990 23.548 310 194 12 48 342 52 333 4.24e-16 80.9
MS.gene38700.t1 AT1G32910 23.718 312 194 13 48 342 52 336 1.32e-15 79.3
MS.gene38700.t1 AT1G28680 24.497 298 202 10 71 358 65 349 2.06e-15 78.2
MS.gene38700.t1 AT1G28680 24.497 298 202 10 71 358 65 349 3.22e-15 78.2
MS.gene38700.t1 AT1G24420 26.419 458 301 17 5 454 4 433 6.58e-15 77.0
MS.gene38700.t1 AT1G24420 26.419 458 301 17 5 454 4 433 6.58e-15 77.0
MS.gene38700.t1 AT1G65450 26.596 188 112 6 166 335 1 180 1.06e-11 65.9
MS.gene38700.t1 AT2G25150 25.497 302 189 13 61 344 60 343 4.75e-11 65.1

Find 107 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGATGTTCCTGTTTA+TGG 0.097445 3.3:-84426998 MS.gene38700:CDS
TCTCTTCCTATGGAATATTT+TGG 0.104357 3.3:-84426653 MS.gene38700:CDS
GAAGATTCTCTCTCTTAAAA+TGG 0.176499 3.3:+84426865 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.204491 3.3:-84426413 MS.gene38700:CDS
ACATGCAGAAACGATGTTTA+TGG 0.230729 3.3:+84427558 None:intergenic
ATTTGCTTTGAGTCTTTGAT+TGG 0.285933 3.3:+84427517 None:intergenic
TTGAATGGAAGGTTGATAAT+TGG 0.293176 3.3:+84426923 None:intergenic
AATCTTTATCGACATTAGTT+TGG 0.302477 3.3:-84426763 MS.gene38700:CDS
TCACCAAGGTTTAATATGTA+TGG 0.306646 3.3:-84426425 MS.gene38700:CDS
AGGAAGAGACGGTTCCATTC+TGG 0.332243 3.3:+84426667 None:intergenic
ATAATTGGACCATAACCTTC+TGG 0.355852 3.3:+84426938 None:intergenic
CAGTTTCTGTGGTTAATCCT+TGG 0.367610 3.3:-84426229 MS.gene38700:CDS
TCAGAGAACAACTTGCAAAA+TGG 0.387167 3.3:-84426705 MS.gene38700:CDS
TTTGCTTTGAGTCTTTGATT+GGG 0.393523 3.3:+84427518 None:intergenic
ACCTGAGAAAATGATGTTGC+TGG 0.395295 3.3:-84426276 MS.gene38700:CDS
TGTCATGATCAAAGAACAAT+TGG 0.397325 3.3:+84427173 None:intergenic
GGATATGCTAACAAATTTGA+TGG 0.406437 3.3:-84426362 MS.gene38700:CDS
ACAGTGGGTGGGACATCAAT+GGG 0.409396 3.3:+84427196 None:intergenic
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.3:-84426947 MS.gene38700:CDS
TATTCAAGTCACTGAATTAG+TGG 0.413794 3.3:-84427110 MS.gene38700:CDS
TGATCCTTACACTGTTATGA+TGG 0.415418 3.3:-84426453 MS.gene38700:CDS
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.3:+84426973 None:intergenic
TCGGTGATGGAACAGCTTAT+TGG 0.419188 3.3:-84427051 MS.gene38700:CDS
ATCACCGATACAATGATTCA+TGG 0.430891 3.3:+84427066 None:intergenic
ATCAACCTATCCACAATAGA+TGG 0.431811 3.3:-84426964 MS.gene38700:CDS
AACGACGGTAGGGGAGTTGC+TGG 0.437091 3.3:-84426603 MS.gene38700:CDS
TTCCAGTTCTATCCCCTCTC+AGG 0.437513 3.3:-84427343 MS.gene38700:CDS
ACTGGAAAAGTGTGAGGGAA+AGG 0.445710 3.3:+84427359 None:intergenic
TGTGAAATGGGACCATAAAC+AGG 0.450206 3.3:+84426986 None:intergenic
AATGGAACCGTCTCTTCCTA+TGG 0.454576 3.3:-84426663 MS.gene38700:CDS
AATATGCTATTTAACACATT+GGG 0.455053 3.3:-84427497 MS.gene38700:CDS
ATCTACACACTATATCCAAA+AGG 0.455900 3.3:-84427461 MS.gene38700:CDS
GACAGTGGGTGGGACATCAA+TGG 0.468510 3.3:+84427195 None:intergenic
AAGATTCTCTCTCTTAAAAT+GGG 0.470281 3.3:+84426866 None:intergenic
CTGGAAAAGTGTGAGGGAAA+GGG 0.483200 3.3:+84427360 None:intergenic
CTACCCATCATAACAGTGTA+AGG 0.483421 3.3:+84426449 None:intergenic
TTGCTTTGAGTCTTTGATTG+GGG 0.486605 3.3:+84427519 None:intergenic
CAGTGGGTGGGACATCAATG+GGG 0.494770 3.3:+84427197 None:intergenic
GAGTTGCTGGAGAATGATCT+AGG 0.495895 3.3:-84426590 MS.gene38700:CDS
CACCAAGGTTTAATATGTAT+GGG 0.497351 3.3:-84426424 MS.gene38700:CDS
AAATATGCTATTTAACACAT+TGG 0.500179 3.3:-84427498 MS.gene38700:CDS
TTCCCATACATATTAAACCT+TGG 0.501336 3.3:+84426422 None:intergenic
TGCAGTGAGTGCTGAAACGA+CGG 0.506205 3.3:-84426618 MS.gene38700:CDS
AGTAACAAGGCGACCTGAGA+GGG 0.513480 3.3:+84427330 None:intergenic
GACAAAGCAATCAGCCATGA+TGG 0.516278 3.3:-84427154 MS.gene38700:CDS
GGTTCTCAATGAAGTGATGT+TGG 0.521934 3.3:+84427404 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.3:-84426407 MS.gene38700:CDS
TGCTTTGAGTCTTTGATTGG+GGG 0.522475 3.3:+84427520 None:intergenic
TGCTGGAGAATGATCTAGGA+TGG 0.522825 3.3:-84426586 MS.gene38700:CDS
AAATCTGTTTGATACACAAA+AGG 0.526852 3.3:+84426482 None:intergenic
ATTTGGAATGGGAAAAGCAT+TGG 0.528592 3.3:-84426396 MS.gene38700:CDS
ATGAATCATTGTATCGGTGA+TGG 0.531203 3.3:-84427064 MS.gene38700:CDS
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.3:+84426974 None:intergenic
CTCCCACCTCCTTCTTGACC+AGG 0.534392 3.3:+84426323 None:intergenic
TGTTGGTTTAGTGATGATGA+TGG 0.539861 3.3:+84427421 None:intergenic
GTTCATGAATTCAGTTTCTG+TGG 0.541865 3.3:-84426240 MS.gene38700:CDS
GGAAAAGTAACATCATATCC+TGG 0.542782 3.3:-84426341 MS.gene38700:CDS
AACTCGTGGTGATGTTTGAA+TGG 0.546781 3.3:+84426908 None:intergenic
GGAAACCATCTATTGTGGAT+AGG 0.548035 3.3:+84426959 None:intergenic
GATCCTTACACTGTTATGAT+GGG 0.549602 3.3:-84426452 MS.gene38700:CDS
ACGAAAACAGTGTAAGAATG+AGG 0.552044 3.3:+84427292 None:intergenic
CGTGGTGATGTTTGAATGGA+AGG 0.554655 3.3:+84426912 None:intergenic
TGAGTGCTGAAACGACGGTA+GGG 0.557272 3.3:-84426613 MS.gene38700:CDS
AAGAACAATTGGACAGTGGG+TGG 0.561237 3.3:+84427184 None:intergenic
ACATCATATCCTGGTCAAGA+AGG 0.563921 3.3:-84426332 MS.gene38700:CDS
TATATTGAAACTTAAAGCAA+AGG 0.566629 3.3:-84426825 MS.gene38700:CDS
TCTACACACTATATCCAAAA+GGG 0.567974 3.3:-84427460 MS.gene38700:CDS
TACAAACAATCGAACCAGAA+TGG 0.569053 3.3:-84426681 MS.gene38700:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.3:-84426577 MS.gene38700:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.3:-84426408 MS.gene38700:CDS
TATCCTGGTCAAGAAGGAGG+TGG 0.578330 3.3:-84426326 MS.gene38700:CDS
ATCAAAGAACAATTGGACAG+TGG 0.579544 3.3:+84427180 None:intergenic
AGTGGGTGGGACATCAATGG+GGG 0.580847 3.3:+84427198 None:intergenic
TCAAAGAACAATTGGACAGT+GGG 0.583509 3.3:+84427181 None:intergenic
AGTAAATCATGATGATAAAG+TGG 0.584307 3.3:-84426537 MS.gene38700:CDS
CACAATAGATGGTTTCCAGA+AGG 0.584706 3.3:-84426953 MS.gene38700:CDS
GAGTGCTGAAACGACGGTAG+GGG 0.587996 3.3:-84426612 MS.gene38700:CDS
GCAGAAACGATGTTTATGGA+AGG 0.592037 3.3:+84427562 None:intergenic
GACCTGAGAGGGGATAGAAC+TGG 0.592219 3.3:+84427341 None:intergenic
CAGAAACGATGTTTATGGAA+GGG 0.593651 3.3:+84427563 None:intergenic
TGCGTAGGTCGGTTGAAGAG+TGG 0.594966 3.3:-84426514 MS.gene38700:CDS
CTTCTGGAAACCATCTATTG+TGG 0.595445 3.3:+84426954 None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG 0.597903 3.3:-84426383 MS.gene38700:CDS
CAAGTCACTGAATTAGTGGA+TGG 0.598818 3.3:-84427106 MS.gene38700:CDS
ATAGTCAAAGACTAAAACCA+AGG 0.608026 3.3:+84426212 None:intergenic
GAATTCCCAAAATATTCCAT+AGG 0.609589 3.3:+84426647 None:intergenic
GTGAGTGCTGAAACGACGGT+AGG 0.617964 3.3:-84426614 MS.gene38700:CDS
ATCCTGGTCAAGAAGGAGGT+GGG 0.618629 3.3:-84426325 MS.gene38700:CDS
CGAAAACAGTGTAAGAATGA+GGG 0.618698 3.3:+84427293 None:intergenic
AAATATTCCATAGGAAGAGA+CGG 0.619061 3.3:+84426656 None:intergenic
AAGACATGGTATGACCATCA+TGG 0.620179 3.3:+84427140 None:intergenic
AGAACAATTGGACAGTGGGT+GGG 0.620480 3.3:+84427185 None:intergenic
GATATAGTGTGTAGATAACA+TGG 0.622938 3.3:+84427468 None:intergenic
GCTGGAGAATGATCTAGGAT+GGG 0.629384 3.3:-84426585 MS.gene38700:CDS
TGTTCCATGAATCATTGTAT+CGG 0.636726 3.3:-84427070 MS.gene38700:CDS
GATAGAACTGGAAAAGTGTG+AGG 0.638589 3.3:+84427353 None:intergenic
ATGATGATAAAGTGGTGCGT+AGG 0.641507 3.3:-84426529 MS.gene38700:CDS
TCATATCCTGGTCAAGAAGG+AGG 0.649960 3.3:-84426329 MS.gene38700:CDS
TGATAAAGTGGTGCGTAGGT+CGG 0.663573 3.3:-84426525 MS.gene38700:CDS
GTAACAAGGCGACCTGAGAG+GGG 0.673777 3.3:+84427331 None:intergenic
TAGTAACAAGGCGACCTGAG+AGG 0.674012 3.3:+84427329 None:intergenic
ATAGAACTGGAAAAGTGTGA+GGG 0.681846 3.3:+84427354 None:intergenic
TGTATCTGTGAACAAACTCG+TGG 0.703902 3.3:+84426894 None:intergenic
TTGAATAGATAACAAAGACA+TGG 0.705301 3.3:+84427126 None:intergenic
AGTGTTTGAGTTTCTCCACG+AGG 0.705768 3.3:+84427383 None:intergenic
TTATGATGGGTAGTTCACCA+AGG 0.706961 3.3:-84426439 MS.gene38700:CDS
TCACTTCATTGAGAACCTCG+TGG 0.716185 3.3:-84427398 MS.gene38700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTGAAACTTAAAGCAA+AGG - chr3.3:84426969-84426988 MS.gene38700:CDS 20.0%
!!! AAATATGCTATTTAACACAT+TGG - chr3.3:84426296-84426315 MS.gene38700:CDS 20.0%
!!! AATATGCTATTTAACACATT+GGG - chr3.3:84426297-84426316 MS.gene38700:CDS 20.0%
! AAATCTGTTTGATACACAAA+AGG + chr3.3:84427315-84427334 None:intergenic 25.0%
! AAGATTCTCTCTCTTAAAAT+GGG + chr3.3:84426931-84426950 None:intergenic 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.3:84427381-84427400 MS.gene38700:CDS 25.0%
! AATCTTTATCGACATTAGTT+TGG - chr3.3:84427031-84427050 MS.gene38700:CDS 25.0%
! AGTAAATCATGATGATAAAG+TGG - chr3.3:84427257-84427276 MS.gene38700:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.3:84426671-84426690 None:intergenic 25.0%
!!! CTTCAGATTTTTTAGTAACA+AGG + chr3.3:84426480-84426499 None:intergenic 25.0%
AAATATTCCATAGGAAGAGA+CGG + chr3.3:84427141-84427160 None:intergenic 30.0%
ATATAGACTCTGCTGAAAAA+TGG + chr3.3:84426954-84426973 None:intergenic 30.0%
ATCTACACACTATATCCAAA+AGG - chr3.3:84426333-84426352 MS.gene38700:CDS 30.0%
CAAAATGATGTTCCTGTTTA+TGG - chr3.3:84426796-84426815 MS.gene38700:CDS 30.0%
CACCAAGGTTTAATATGTAT+GGG - chr3.3:84427370-84427389 MS.gene38700:CDS 30.0%
GAAGATTCTCTCTCTTAAAA+TGG + chr3.3:84426932-84426951 None:intergenic 30.0%
GAATTCCCAAAATATTCCAT+AGG + chr3.3:84427150-84427169 None:intergenic 30.0%
GATATAGTGTGTAGATAACA+TGG + chr3.3:84426329-84426348 None:intergenic 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.3:84427432-84427451 MS.gene38700:CDS 30.0%
TATTCAAGTCACTGAATTAG+TGG - chr3.3:84426684-84426703 MS.gene38700:CDS 30.0%
TCACCAAGGTTTAATATGTA+TGG - chr3.3:84427369-84427388 MS.gene38700:CDS 30.0%
TCTACACACTATATCCAAAA+GGG - chr3.3:84426334-84426353 MS.gene38700:CDS 30.0%
TGTCATGATCAAAGAACAAT+TGG + chr3.3:84426624-84426643 None:intergenic 30.0%
TGTTCCATGAATCATTGTAT+CGG - chr3.3:84426724-84426743 MS.gene38700:CDS 30.0%
TTCCCATACATATTAAACCT+TGG + chr3.3:84427375-84427394 None:intergenic 30.0%
! ATTTGCTTTGAGTCTTTGAT+TGG + chr3.3:84426280-84426299 None:intergenic 30.0%
! CTCTTCCTATGGAATATTTT+GGG - chr3.3:84427142-84427161 MS.gene38700:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.3:84427387-84427406 MS.gene38700:CDS 30.0%
! TCTCTTCCTATGGAATATTT+TGG - chr3.3:84427141-84427160 MS.gene38700:CDS 30.0%
! TTTGCTTTGAGTCTTTGATT+GGG + chr3.3:84426279-84426298 None:intergenic 30.0%
!! TTGAATGGAAGGTTGATAAT+TGG + chr3.3:84426874-84426893 None:intergenic 30.0%
ACATGCAGAAACGATGTTTA+TGG + chr3.3:84426239-84426258 None:intergenic 35.0%
ACGAAAACAGTGTAAGAATG+AGG + chr3.3:84426505-84426524 None:intergenic 35.0%
ATAATTGGACCATAACCTTC+TGG + chr3.3:84426859-84426878 None:intergenic 35.0%
ATCAAAGAACAATTGGACAG+TGG + chr3.3:84426617-84426636 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.3:84426830-84426849 MS.gene38700:CDS 35.0%
ATCACCGATACAATGATTCA+TGG + chr3.3:84426731-84426750 None:intergenic 35.0%
CAGAAACGATGTTTATGGAA+GGG + chr3.3:84426234-84426253 None:intergenic 35.0%
CGAAAACAGTGTAAGAATGA+GGG + chr3.3:84426504-84426523 None:intergenic 35.0%
GATCCTTACACTGTTATGAT+GGG - chr3.3:84427342-84427361 MS.gene38700:CDS 35.0%
GGAAAAGTAACATCATATCC+TGG - chr3.3:84427453-84427472 MS.gene38700:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.3:84427386-84427405 MS.gene38700:CDS 35.0%
GTTCATGAATTCAGTTTCTG+TGG - chr3.3:84427554-84427573 MS.gene38700:CDS 35.0%
TACAAACAATCGAACCAGAA+TGG - chr3.3:84427113-84427132 MS.gene38700:CDS 35.0%
TCAAAGAACAATTGGACAGT+GGG + chr3.3:84426616-84426635 None:intergenic 35.0%
TCAGAGAACAACTTGCAAAA+TGG - chr3.3:84427089-84427108 MS.gene38700:CDS 35.0%
TGATCCTTACACTGTTATGA+TGG - chr3.3:84427341-84427360 MS.gene38700:CDS 35.0%
! ATAGAACTGGAAAAGTGTGA+GGG + chr3.3:84426443-84426462 None:intergenic 35.0%
! ATGAATCATTGTATCGGTGA+TGG - chr3.3:84426730-84426749 MS.gene38700:CDS 35.0%
! ATTTGGAATGGGAAAAGCAT+TGG - chr3.3:84427398-84427417 MS.gene38700:CDS 35.0%
! TTGCTTTGAGTCTTTGATTG+GGG + chr3.3:84426278-84426297 None:intergenic 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.3:84426823-84426842 None:intergenic 35.0%
!! TGTTGGTTTAGTGATGATGA+TGG + chr3.3:84426376-84426395 None:intergenic 35.0%
!! TTAGTGGATGGTGTTTTCAT+TGG - chr3.3:84426700-84426719 MS.gene38700:CDS 35.0%
!! TTTTGAAGAGAAGTCCCTTT+TGG + chr3.3:84426351-84426370 None:intergenic 35.0%
AAGACATGGTATGACCATCA+TGG + chr3.3:84426657-84426676 None:intergenic 40.0%
ACATCATATCCTGGTCAAGA+AGG - chr3.3:84427462-84427481 MS.gene38700:CDS 40.0%
ACCTGAGAAAATGATGTTGC+TGG - chr3.3:84427518-84427537 MS.gene38700:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.3:84426847-84426866 MS.gene38700:CDS 40.0%
ATGATGATAAAGTGGTGCGT+AGG - chr3.3:84427265-84427284 MS.gene38700:CDS 40.0%
CAAGTCACTGAATTAGTGGA+TGG - chr3.3:84426688-84426707 MS.gene38700:CDS 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.3:84426841-84426860 MS.gene38700:CDS 40.0%
CAGTTTCTGTGGTTAATCCT+TGG - chr3.3:84427565-84427584 MS.gene38700:CDS 40.0%
CTACCCATCATAACAGTGTA+AGG + chr3.3:84427348-84427367 None:intergenic 40.0%
GCAGAAACGATGTTTATGGA+AGG + chr3.3:84426235-84426254 None:intergenic 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.3:84426838-84426857 None:intergenic 40.0%
GGTTCTCAATGAAGTGATGT+TGG + chr3.3:84426393-84426412 None:intergenic 40.0%
TGTATCTGTGAACAAACTCG+TGG + chr3.3:84426903-84426922 None:intergenic 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.3:84426811-84426830 None:intergenic 40.0%
! AACTCGTGGTGATGTTTGAA+TGG + chr3.3:84426889-84426908 None:intergenic 40.0%
! CTTCTGGAAACCATCTATTG+TGG + chr3.3:84426843-84426862 None:intergenic 40.0%
! GATAGAACTGGAAAAGTGTG+AGG + chr3.3:84426444-84426463 None:intergenic 40.0%
! TCCAGCAACATCATTTTCTC+AGG + chr3.3:84427522-84427541 None:intergenic 40.0%
! TGCTTTGAGTCTTTGATTGG+GGG + chr3.3:84426277-84426296 None:intergenic 40.0%
!! AAAGCATTGGCAGTTAGAAG+TGG - chr3.3:84427411-84427430 MS.gene38700:CDS 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.3:84426824-84426843 None:intergenic 40.0%
!! TTATGATGGGTAGTTCACCA+AGG - chr3.3:84427355-84427374 MS.gene38700:CDS 40.0%
AAGAACAATTGGACAGTGGG+TGG + chr3.3:84426613-84426632 None:intergenic 45.0%
AATGGAACCGTCTCTTCCTA+TGG - chr3.3:84427131-84427150 MS.gene38700:CDS 45.0%
AGAACAATTGGACAGTGGGT+GGG + chr3.3:84426612-84426631 None:intergenic 45.0%
GACAAAGCAATCAGCCATGA+TGG - chr3.3:84426640-84426659 MS.gene38700:CDS 45.0%
GAGTCTTTGATTGGGGGATA+AGG + chr3.3:84426271-84426290 None:intergenic 45.0%
GAGTTGCTGGAGAATGATCT+AGG - chr3.3:84427204-84427223 MS.gene38700:CDS 45.0%
GTCGATTGCAAAAACAGTCG+CGG - chr3.3:84426523-84426542 MS.gene38700:CDS 45.0%
TCACTTCATTGAGAACCTCG+TGG - chr3.3:84426396-84426415 MS.gene38700:CDS 45.0%
TCATATCCTGGTCAAGAAGG+AGG - chr3.3:84427465-84427484 MS.gene38700:CDS 45.0%
TGATAAAGTGGTGCGTAGGT+CGG - chr3.3:84427269-84427288 MS.gene38700:CDS 45.0%
TGCTGGAGAATGATCTAGGA+TGG - chr3.3:84427208-84427227 MS.gene38700:CDS 45.0%
! ACTGGAAAAGTGTGAGGGAA+AGG + chr3.3:84426438-84426457 None:intergenic 45.0%
! AGTGTTTGAGTTTCTCCACG+AGG + chr3.3:84426414-84426433 None:intergenic 45.0%
! CGTGGTGATGTTTGAATGGA+AGG + chr3.3:84426885-84426904 None:intergenic 45.0%
! CTGGAAAAGTGTGAGGGAAA+GGG + chr3.3:84426437-84426456 None:intergenic 45.0%
! GCTGGAGAATGATCTAGGAT+GGG - chr3.3:84427209-84427228 MS.gene38700:CDS 45.0%
!! TCGGTGATGGAACAGCTTAT+TGG - chr3.3:84426743-84426762 MS.gene38700:CDS 45.0%
ACAGTGGGTGGGACATCAAT+GGG + chr3.3:84426601-84426620 None:intergenic 50.0%
AGGAAGAGACGGTTCCATTC+TGG + chr3.3:84427130-84427149 None:intergenic 50.0%
AGTAACAAGGCGACCTGAGA+GGG + chr3.3:84426467-84426486 None:intergenic 50.0%
ATCCTGGTCAAGAAGGAGGT+GGG - chr3.3:84427469-84427488 MS.gene38700:CDS 50.0%
GGGGGTGAGGATATCATCTA+TGG + chr3.3:84426581-84426600 None:intergenic 50.0%
TAGTAACAAGGCGACCTGAG+AGG + chr3.3:84426468-84426487 None:intergenic 50.0%
TATCCTGGTCAAGAAGGAGG+TGG - chr3.3:84427468-84427487 MS.gene38700:CDS 50.0%
TTCCAGTTCTATCCCCTCTC+AGG - chr3.3:84426451-84426470 MS.gene38700:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.3:84427217-84427236 MS.gene38700:CDS 50.0%
!! TGAGTGCTGAAACGACGGTA+GGG - chr3.3:84427181-84427200 MS.gene38700:CDS 50.0%
!! TGCAGTGAGTGCTGAAACGA+CGG - chr3.3:84427176-84427195 MS.gene38700:CDS 50.0%
AGTGGGTGGGACATCAATGG+GGG + chr3.3:84426599-84426618 None:intergenic 55.0%
CAGTGGGTGGGACATCAATG+GGG + chr3.3:84426600-84426619 None:intergenic 55.0%
GACAGTGGGTGGGACATCAA+TGG + chr3.3:84426602-84426621 None:intergenic 55.0%
GACCTGAGAGGGGATAGAAC+TGG + chr3.3:84426456-84426475 None:intergenic 55.0%
GTAACAAGGCGACCTGAGAG+GGG + chr3.3:84426466-84426485 None:intergenic 55.0%
TGCGTAGGTCGGTTGAAGAG+TGG - chr3.3:84427280-84427299 MS.gene38700:CDS 55.0%
!! GAGTGCTGAAACGACGGTAG+GGG - chr3.3:84427182-84427201 MS.gene38700:CDS 55.0%
!! GTGAGTGCTGAAACGACGGT+AGG - chr3.3:84427180-84427199 MS.gene38700:CDS 55.0%
AACGACGGTAGGGGAGTTGC+TGG - chr3.3:84427191-84427210 MS.gene38700:CDS 60.0%
CTCCCACCTCCTTCTTGACC+AGG + chr3.3:84427474-84427493 None:intergenic 60.0%
GTGGGACATCAATGGGGGTG+AGG + chr3.3:84426594-84426613 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 84426223 84427593 84426223 ID=MS.gene38700
chr3.3 mRNA 84426223 84427593 84426223 ID=MS.gene38700.t1;Parent=MS.gene38700
chr3.3 exon 84426223 84427593 84426223 ID=MS.gene38700.t1.exon1;Parent=MS.gene38700.t1
chr3.3 CDS 84426223 84427593 84426223 ID=cds.MS.gene38700.t1;Parent=MS.gene38700.t1
Gene Sequence

>MS.gene38700

ATGAGGATCCCTTCCATAAACATCGTTTCTGCATGTTTTATTAAACCTTATCCCCCAATCAAAGACTCAAAGCAAATATGCTATTTAACACATTGGGATATTGCCATGTTATCTACACACTATATCCAAAAGGGACTTCTCTTCAAAAAACCATCATCATCACTAAACCAACATCACTTCATTGAGAACCTCGTGGAGAAACTCAAACACTCCCTTTCCCTCACACTTTTCCAGTTCTATCCCCTCTCAGGTCGCCTTGTTACTAAAAAATCTGAAGACCCTCATTCTTACACTGTTTTCGTCGATTGCAAAAACAGTCGCGGAGCTAGATTTATTCATGCAACTTTAGATATCACCATAGATGATATCCTCACCCCCATTGATGTCCCACCCACTGTCCAATTGTTCTTTGATCATGACAAAGCAATCAGCCATGATGGTCATACCATGTCTTTGTTATCTATTCAAGTCACTGAATTAGTGGATGGTGTTTTCATTGGTTGTTCCATGAATCATTGTATCGGTGATGGAACAGCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATGTTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATTATCAACCTTCCATTCAAACATCACCACGAGTTTGTTCACAGATACAAAATACCCATTTTAAGAGAGAGAATCTTCCATTTTTCAGCAGAGTCTATATTGAAACTTAAAGCAAAGGCAAATGCAGAGTCTAATACAAACAAAATTTCTTCGTTTCAATCTTTATCGACATTAGTTTGGAGATCTATAACTCGTGCACGTCAACTACAACACGATCAGAGAACAACTTGCAAAATGGCTACAAACAATCGAACCAGAATGGAACCGTCTCTTCCTATGGAATATTTTGGGAATTCACTTTATGCAGTGAGTGCTGAAACGACGGTAGGGGAGTTGCTGGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATCTAGCTGTAGTAAATCATGATGATAAAGTGGTGCGTAGGTCGGTTGAAGAGTGGTTTCGTTCTCCTTTTGTGTATCAAACAGATTTGTTTTTTGATCCTTACACTGTTATGATGGGTAGTTCACCAAGGTTTAATATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCTGGTCAAGAAGGAGGTGGGAGCGTTGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGTTGCTGGAAGCAGATGAAGAGTTCATGAATTCAGTTTCTGTGGTTAATCCTTGGTTTTAG
Protein sequence

>MS.gene38700.t1

MRIPSINIVSACFIKPYPPIKDSKQICYLTHWDIAMLSTHYIQKGLLFKKPSSSLNQHHFIENLVEKLKHSLSLTLFQFYPLSGRLVTKKSEDPHSYTVFVDCKNSRGARFIHATLDITIDDILTPIDVPPTVQLFFDHDKAISHDGHTMSLLSIQVTELVDGVFIGCSMNHCIGDGTAYWNFFNTFSEIFQNDVPVYGPISHQPIHNRWFPEGYGPIINLPFKHHHEFVHRYKIPILRERIFHFSAESILKLKAKANAESNTNKISSFQSLSTLVWRSITRARQLQHDQRTTCKMATNNRTRMEPSLPMEYFGNSLYAVSAETTVGELLENDLGWAAWKIHLAVVNHDDKVVRRSVEEWFRSPFVYQTDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGQEGGGSVDLEVCLSPEKMMLLEADEEFMNSVSVVNPWF