AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012957

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012957.t1 MTR_3g102020 94.967 457 23 0 1 457 1 457 0.0 904
MS.gene012957.t1 MTR_3g101960 89.013 446 48 1 1 446 1 445 0.0 840
MS.gene012957.t1 MTR_3g102010 85.033 461 60 3 1 457 2 457 0.0 809
MS.gene012957.t1 MTR_3g101970 84.292 452 57 1 1 452 1 438 0.0 802
MS.gene012957.t1 MTR_3g101900 68.653 453 136 3 1 452 2 449 0.0 637
MS.gene012957.t1 MTR_3g101930 66.520 454 147 3 1 450 2 454 0.0 625
MS.gene012957.t1 MTR_3g102030 79.874 318 38 2 87 404 29 320 0.0 524
MS.gene012957.t1 MTR_3g070170 39.837 492 217 14 2 447 8 466 2.04e-104 319
MS.gene012957.t1 MTR_4g015180 39.604 505 233 12 1 451 1 487 2.19e-99 306
MS.gene012957.t1 MTR_5g081960 39.044 502 231 12 1 447 1 482 1.26e-98 305
MS.gene012957.t1 MTR_8g041770 39.408 439 247 10 16 451 19 441 3.80e-94 291
MS.gene012957.t1 MTR_4g094842 38.646 458 252 11 4 447 2 444 3.61e-91 284
MS.gene012957.t1 MTR_4g022800 38.409 440 241 11 25 451 20 442 1.70e-89 280
MS.gene012957.t1 MTR_4g022825 38.724 439 241 10 24 451 19 440 4.91e-89 278
MS.gene012957.t1 MTR_4g022720 39.723 433 240 9 24 451 19 435 5.78e-89 278
MS.gene012957.t1 MTR_4g094835 38.605 430 242 8 25 447 28 442 3.89e-88 276
MS.gene012957.t1 MTR_0190s0020 38.318 428 247 8 28 451 28 442 1.24e-87 275
MS.gene012957.t1 MTR_4g094838 38.462 429 228 8 28 447 27 428 6.45e-87 273
MS.gene012957.t1 MTR_3g088420 38.710 434 241 10 24 451 19 433 6.93e-87 272
MS.gene012957.t1 MTR_4g022670 38.497 439 243 10 24 451 19 441 1.13e-85 270
MS.gene012957.t1 MTR_7g026860 37.358 439 245 11 24 451 19 438 1.49e-82 261
MS.gene012957.t1 MTR_3g026310 37.698 443 238 12 29 451 24 448 9.35e-81 257
MS.gene012957.t1 MTR_4g094828 38.063 444 240 14 26 452 26 451 1.06e-79 254
MS.gene012957.t1 MTR_4g022290 36.905 420 232 11 24 430 25 424 1.29e-77 249
MS.gene012957.t1 MTR_8g079710 38.771 423 223 17 25 432 25 426 2.62e-74 240
MS.gene012957.t1 MTR_4g094832 42.120 349 187 8 106 447 86 426 4.49e-73 236
MS.gene012957.t1 MTR_4g022760 36.176 387 211 11 24 387 19 392 1.14e-66 219
MS.gene012957.t1 MTR_6g045553 31.875 480 275 12 5 450 13 474 3.95e-60 204
MS.gene012957.t1 MTR_6g016815 31.090 431 212 11 24 451 33 381 1.11e-47 168
MS.gene012957.t1 MTR_8g040780 36.607 224 137 4 235 454 14 236 4.23e-40 144
MS.gene012957.t1 MTR_8g075610 27.411 394 239 14 69 441 47 414 4.67e-33 129
MS.gene012957.t1 MTR_8g096530 31.329 316 177 11 63 359 53 347 3.51e-32 127
MS.gene012957.t1 MTR_4g007540 24.837 459 288 17 6 441 3 427 1.14e-31 126
MS.gene012957.t1 MTR_2g064495 26.753 385 256 13 69 441 56 426 7.35e-31 124
MS.gene012957.t1 MTR_2g105330 26.494 385 257 13 69 441 56 426 2.70e-30 122
MS.gene012957.t1 MTR_3g098980 30.033 303 185 9 69 359 66 353 1.03e-29 120
MS.gene012957.t1 MTR_7g026850 41.667 156 79 4 43 197 21 165 2.05e-29 114
MS.gene012957.t1 MTR_3g049150 27.233 459 252 19 22 441 25 440 2.05e-29 120
MS.gene012957.t1 MTR_8g040760 35.135 222 132 5 4 224 2 212 3.22e-29 114
MS.gene012957.t1 MTR_4g127650 26.464 461 252 19 19 437 30 445 7.81e-28 115
MS.gene012957.t1 MTR_7g026770 37.853 177 89 3 156 332 132 287 9.82e-28 112
MS.gene012957.t1 MTR_3g026950 24.468 470 272 18 17 446 20 446 1.27e-27 115
MS.gene012957.t1 MTR_4g097090 26.577 444 263 16 29 437 30 445 1.07e-25 109
MS.gene012957.t1 MTR_4g094825 38.667 150 81 2 302 451 253 391 3.67e-25 107
MS.gene012957.t1 MTR_4g094825 30.453 243 138 7 29 255 92 319 4.63e-22 98.2
MS.gene012957.t1 MTR_6g053810 32.168 286 176 12 70 345 72 349 3.30e-24 105
MS.gene012957.t1 MTR_3g112180 26.812 414 242 16 14 400 11 390 1.77e-23 102
MS.gene012957.t1 MTR_7g027175 29.841 315 195 10 57 359 56 356 2.63e-23 102
MS.gene012957.t1 MTR_6g034495 43.220 118 63 3 80 194 45 161 4.54e-23 95.9
MS.gene012957.t1 MTR_5g076700 25.117 426 277 17 33 437 27 431 2.01e-22 99.8
MS.gene012957.t1 MTR_7g114470 25.532 423 280 16 33 437 28 433 6.14e-22 98.6
MS.gene012957.t1 MTR_1g081660 25.679 405 263 14 66 454 70 452 1.07e-21 97.4
MS.gene012957.t1 MTR_7g114550 26.364 440 277 20 33 448 27 443 4.10e-21 95.9
MS.gene012957.t1 MTR_3g071320 26.933 375 235 14 3 359 2 355 5.06e-21 95.5
MS.gene012957.t1 MTR_1g040195 23.944 426 281 16 48 451 16 420 6.85e-21 95.1
MS.gene012957.t1 MTR_1g040175 27.248 367 230 17 66 420 61 402 3.01e-20 93.2
MS.gene012957.t1 MTR_4g055170 27.046 281 178 8 69 335 69 336 4.95e-20 92.8
MS.gene012957.t1 MTR_3g465980 27.458 295 190 10 53 335 52 334 6.10e-20 92.4
MS.gene012957.t1 MTR_7g086000 27.020 396 237 19 72 432 63 441 1.26e-19 91.7
MS.gene012957.t1 MTR_7g114560 25.571 438 285 19 33 449 27 444 1.29e-18 88.6
MS.gene012957.t1 MTR_3g465990 26.780 295 192 10 53 335 52 334 5.34e-18 86.7
MS.gene012957.t1 MTR_6g034485 35.897 117 68 2 206 318 2 115 1.37e-17 79.3
MS.gene012957.t1 MTR_3g465930 27.899 276 177 8 69 332 69 334 1.98e-17 84.7
MS.gene012957.t1 MTR_7g114960 25.815 399 265 11 66 447 60 444 5.48e-17 83.6
MS.gene012957.t1 MTR_7g084940 24.359 312 205 10 65 361 65 360 7.89e-17 82.8
MS.gene012957.t1 MTR_3g465950 28.302 265 169 7 70 322 69 324 9.51e-17 82.8
MS.gene012957.t1 MTR_7g014160 24.788 472 285 18 18 442 19 467 1.05e-16 82.8
MS.gene012957.t1 MTR_2g015430 25.000 296 204 9 72 359 61 346 1.25e-16 82.4
MS.gene012957.t1 MTR_4g013050 23.940 401 231 16 67 441 51 403 1.37e-16 82.0
MS.gene012957.t1 MTR_1g040215 27.835 291 190 11 66 348 61 339 2.50e-16 81.3
MS.gene012957.t1 MTR_3g465910 28.674 279 167 9 70 332 69 331 5.46e-16 80.5
MS.gene012957.t1 MTR_6g015850 25.420 417 249 15 61 442 62 451 6.76e-16 80.1
MS.gene012957.t1 MTR_5g026320 24.586 423 262 17 48 446 59 448 1.63e-15 79.0
MS.gene012957.t1 MTR_6g015830 24.450 409 266 14 61 442 62 454 1.02e-14 76.6
MS.gene012957.t1 MTR_7g026830 50.000 68 33 1 112 178 28 95 2.39e-14 68.9
MS.gene012957.t1 MTR_7g056087 23.670 376 248 14 6 362 4 359 3.51e-14 74.7
MS.gene012957.t1 MTR_7g014620 24.374 439 280 21 29 442 15 426 4.01e-14 74.3
MS.gene012957.t1 MTR_1g040315 26.645 304 197 11 33 326 26 313 4.57e-14 74.3
MS.gene012957.t1 MTR_3g049140 25.980 204 133 5 243 432 27 226 8.15e-14 71.6
MS.gene012957.t1 MTR_7g085930 23.358 411 262 13 65 437 55 450 1.35e-13 73.2
MS.gene012957.t1 MTR_7g013850 23.515 421 257 15 32 403 32 436 2.12e-13 72.4
MS.gene012957.t1 MTR_7g014200 24.762 420 274 18 15 403 16 424 2.74e-13 72.0
MS.gene012957.t1 MTR_3g450710 23.151 311 176 11 63 337 59 342 2.21e-12 69.3
MS.gene012957.t1 MTR_6g015315 24.818 411 265 19 54 437 48 441 3.22e-12 68.6
MS.gene012957.t1 MTR_8g079970 24.784 347 205 14 6 331 3 314 4.56e-12 68.2
MS.gene012957.t1 MTR_7g084910 23.765 324 207 10 18 316 10 318 5.04e-12 67.8
MS.gene012957.t1 MTR_5g090830 23.779 307 187 15 64 343 61 347 8.00e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012957.t1 AT5G42830 56.885 443 182 2 13 452 14 450 0.0 527
MS.gene012957.t1 AT5G07850 54.324 451 204 2 2 451 4 453 0.0 515
MS.gene012957.t1 AT5G07860 54.000 450 197 5 4 450 6 448 3.18e-169 484
MS.gene012957.t1 AT5G07870 50.649 462 212 3 2 451 4 461 3.43e-165 474
MS.gene012957.t1 AT2G39980 40.254 472 213 9 29 451 29 480 3.39e-104 318
MS.gene012957.t1 AT5G67150 39.434 459 256 11 3 452 2 447 2.83e-101 310
MS.gene012957.t1 AT5G01210 38.900 491 238 12 1 451 1 469 1.04e-92 289
MS.gene012957.t1 AT3G50270 35.345 464 273 12 1 452 1 449 6.81e-83 263
MS.gene012957.t1 AT3G50280 34.956 452 272 11 4 447 2 439 1.88e-77 248
MS.gene012957.t1 AT3G50300 32.969 458 279 9 6 450 4 446 5.23e-75 242
MS.gene012957.t1 AT5G38130 34.932 438 259 12 6 434 24 444 1.49e-72 236
MS.gene012957.t1 AT5G67160 35.198 429 251 11 28 452 28 433 2.88e-71 232
MS.gene012957.t1 AT5G23940 33.696 460 277 11 6 449 3 450 2.59e-66 220
MS.gene012957.t1 AT3G50280 37.785 307 180 6 147 447 8 309 7.76e-56 188
MS.gene012957.t1 AT3G50290 37.838 259 148 6 148 397 15 269 1.45e-44 157
MS.gene012957.t1 AT2G19070 29.478 441 254 18 29 437 25 440 7.56e-35 135
MS.gene012957.t1 AT5G48930 25.821 457 286 16 6 441 3 427 2.68e-33 130
MS.gene012957.t1 AT5G41040 29.526 359 206 15 63 400 63 395 3.77e-32 127
MS.gene012957.t1 AT5G41040 29.526 359 206 15 63 400 79 411 5.45e-32 127
MS.gene012957.t1 AT3G48720 26.790 433 268 17 1 421 1 396 6.00e-24 103
MS.gene012957.t1 AT2G40230 27.177 379 209 13 64 410 54 397 8.01e-23 100
MS.gene012957.t1 AT5G63560 29.167 312 185 12 63 359 55 345 2.84e-22 99.0
MS.gene012957.t1 AT1G65450 26.935 323 201 12 57 359 60 367 3.16e-21 96.3
MS.gene012957.t1 AT1G27620 26.984 315 182 10 69 359 58 348 3.44e-20 93.2
MS.gene012957.t1 AT1G28680 26.351 296 199 11 72 359 65 349 3.75e-20 92.4
MS.gene012957.t1 AT5G57840 26.190 420 251 16 46 437 39 427 3.76e-20 92.8
MS.gene012957.t1 AT1G28680 26.351 296 199 11 72 359 65 349 5.30e-20 92.4
MS.gene012957.t1 AT3G26040 26.763 482 269 24 6 455 3 432 4.82e-19 89.7
MS.gene012957.t1 AT2G25150 27.667 300 185 12 62 345 60 343 6.38e-16 80.1
MS.gene012957.t1 AT5G16410 22.747 466 280 17 24 456 56 474 2.38e-15 78.6
MS.gene012957.t1 AT1G78990 24.430 307 196 11 48 343 52 333 2.68e-15 78.2
MS.gene012957.t1 AT1G03390 25.310 403 252 18 54 432 64 441 5.66e-15 77.4
MS.gene012957.t1 AT5G07080 25.448 279 172 11 70 330 71 331 6.39e-15 77.0
MS.gene012957.t1 AT1G65450 25.837 209 133 6 167 359 1 203 1.77e-13 71.2
MS.gene012957.t1 AT3G03480 25.672 335 204 15 43 356 46 356 4.58e-13 71.2
MS.gene012957.t1 AT2G23510 27.059 255 170 7 69 317 69 313 8.39e-13 70.5
MS.gene012957.t1 AT1G03940 23.239 426 266 21 57 442 54 458 1.90e-12 69.3
MS.gene012957.t1 AT3G29590 23.514 387 254 14 76 437 68 437 2.90e-12 68.9
MS.gene012957.t1 AT5G23970 24.622 463 280 19 6 447 3 417 1.20e-11 67.0

Find 104 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.4:-89778742 MS.gene012957:CDS
CATTCAAGTTACTGAATTAT+TGG 0.137906 3.4:-89779199 MS.gene012957:CDS
AATATGTATGGGAATGAATT+TGG 0.211414 3.4:-89778502 MS.gene012957:CDS
AATGTGATAAATGGCATATT+TGG 0.269841 3.4:+89779592 None:intergenic
AATATTCTCTCTCTCAAAAT+AGG 0.288986 3.4:+89778955 None:intergenic
AGGAAGAGGTGGTTCCATTC+TGG 0.292655 3.4:+89778756 None:intergenic
TAATATCCCAATGTGATAAA+TGG 0.297305 3.4:+89779583 None:intergenic
AATCTTTATCAGCACTAGTT+TGG 0.302845 3.4:-89778852 MS.gene012957:CDS
TCAACGGACAACTTGCAAAT+TGG 0.320331 3.4:-89778794 MS.gene012957:CDS
CTACCCATCATCACAGTATA+AGG 0.343091 3.4:+89778538 None:intergenic
CATTCTGGTTCGGTTGTTTA+CGG 0.353477 3.4:+89778771 None:intergenic
GGAAACCATCTATTGTGAAT+AGG 0.358486 3.4:+89779048 None:intergenic
ATAAACCTATTCACAATAGA+TGG 0.359431 3.4:-89779053 MS.gene012957:CDS
TCAAAGAATGATTGAACAAT+AGG 0.361615 3.4:+89779270 None:intergenic
ATGATTGGACCATAACCTTC+TGG 0.364910 3.4:+89779027 None:intergenic
AGATGAAGAGTTCATGAATT+CGG 0.367038 3.4:-89778338 MS.gene012957:CDS
ATCTGAGAAAATGATGTTGC+TGG 0.375240 3.4:-89778365 MS.gene012957:CDS
TCACCAAGGTTCAATATGTA+TGG 0.377799 3.4:-89778514 MS.gene012957:CDS
TTCACCACTCACCGCATAGA+TGG 0.382856 3.4:+89778714 None:intergenic
TGAATAGGTTTATGTGAAAT+CGG 0.391414 3.4:+89779063 None:intergenic
TTGGTGATGGAACTGGTTAT+TGG 0.394500 3.4:-89779140 MS.gene012957:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3.4:+89779155 None:intergenic
GGATATGCTAACAAATTTGA+TGG 0.406437 3.4:-89778451 MS.gene012957:CDS
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.4:-89779036 MS.gene012957:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.425196 3.4:-89779432 MS.gene012957:CDS
ACAATAGGTGGCACATCAAC+AGG 0.427451 3.4:+89779285 None:intergenic
AATATGCCATTTATCACATT+GGG 0.437912 3.4:-89779589 MS.gene012957:CDS
AGTGTTTCAGTTTCTCTACT+AGG 0.438345 3.4:+89779472 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3.4:+89779493 None:intergenic
ATTTGGTTCAAGTCTTCGAT+TGG 0.440400 3.4:+89779609 None:intergenic
TGATCCTTATACTGTGATGA+TGG 0.442813 3.4:-89778542 MS.gene012957:CDS
TTTGGTTCAAGTCTTCGATT+GGG 0.445265 3.4:+89779610 None:intergenic
CTTCCTCCTCCTTCTTGACC+TGG 0.449239 3.4:+89778412 None:intergenic
CATTGTATTGGTGATGGAAC+TGG 0.455215 3.4:-89779147 MS.gene012957:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.4:+89779389 None:intergenic
AATGGAAAAGTGTGAGGGAA+AGG 0.463707 3.4:+89779448 None:intergenic
TTGAAAGGAAGGTTGATGAT+TGG 0.467597 3.4:+89779012 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.4:+89778742 None:intergenic
GAGGTGGTTCCATTCTGGTT+CGG 0.471982 3.4:+89778761 None:intergenic
TGATAAAGTGGTGCGAAATT+CGG 0.474906 3.4:-89778614 MS.gene012957:CDS
AGAGTTCATGAATTCGGTTT+CGG 0.476951 3.4:-89778332 MS.gene012957:CDS
GAATTCCCAAAATATTCCTT+AGG 0.479625 3.4:+89778736 None:intergenic
CATCATAATTTGCAATAGCC+AGG 0.479956 3.4:+89778635 None:intergenic
CAATAATTCAGTAACTTGAA+TGG 0.486762 3.4:+89779200 None:intergenic
AACAACGGTAGGGGAATTGC+TGG 0.488208 3.4:-89778692 MS.gene012957:CDS
CGTCGTGATGTTTGAAAGGA+AGG 0.488568 3.4:+89779001 None:intergenic
ATGGAAAAGTGTGAGGGAAA+GGG 0.490102 3.4:+89779449 None:intergenic
AAATATGCCATTTATCACAT+TGG 0.490158 3.4:-89779590 MS.gene012957:CDS
GGTTTATGTGAAATCGGAAC+AGG 0.490426 3.4:+89779069 None:intergenic
ATCTGCATACTATATCCAAA+AGG 0.491658 3.4:-89779553 MS.gene012957:CDS
TGTTCCATGAATCATTGTAT+TGG 0.493564 3.4:-89779159 MS.gene012957:CDS
AGGTGAGAGAATATCATTGA+TGG 0.504727 3.4:+89779305 None:intergenic
GAATTGCTGGAAAATGATCT+AGG 0.510030 3.4:-89778679 MS.gene012957:CDS
CAAGTTACTGAATTATTGGA+TGG 0.511663 3.4:-89779195 MS.gene012957:CDS
TGCTGGAAAATGATCTAGGA+TGG 0.517189 3.4:-89778675 MS.gene012957:CDS
TTCCCATACATATTGAACCT+TGG 0.519386 3.4:+89778511 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.4:-89778496 MS.gene012957:CDS
AGATCCATTCGATACACAAA+AGG 0.526309 3.4:+89778571 None:intergenic
ATTTGGAATGGGAAAAGCAT+TGG 0.528592 3.4:-89778485 MS.gene012957:CDS
TGGTTTAGTGATGATGATGT+TGG 0.530066 3.4:+89779513 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.4:-89779153 MS.gene012957:CDS
GCATGAATAAATTTAGCTCC+AGG 0.532002 3.4:+89779342 None:intergenic
GGAAAAGTAACATCATATCC+AGG 0.542909 3.4:-89778430 MS.gene012957:CDS
AACTCGTCGTGATGTTTGAA+AGG 0.543380 3.4:+89778997 None:intergenic
CACAGAGCTGTCAACCATGA+TGG 0.543528 3.4:-89779243 MS.gene012957:CDS
CACAATAGATGGTTTCCAGA+AGG 0.548245 3.4:-89779042 MS.gene012957:CDS
CGTAAACAACCGAACCAGAA+TGG 0.549236 3.4:-89778770 MS.gene012957:CDS
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.4:+89779419 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.4:+89779430 None:intergenic
TGCAAATTATGATGATAAAG+TGG 0.556314 3.4:-89778626 MS.gene012957:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.557831 3.4:-89778666 MS.gene012957:CDS
CTCATCAAAGACTAAAACAA+AGG 0.560969 3.4:+89778301 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.4:-89778513 MS.gene012957:CDS
TCTGCATACTATATCCAAAA+GGG 0.562084 3.4:-89779552 MS.gene012957:CDS
GTCATTTACAACATGGTCAA+CGG 0.571152 3.4:-89778810 MS.gene012957:CDS
AATGGAACCACCTCTTCCTA+AGG 0.571584 3.4:-89778752 MS.gene012957:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.4:-89778497 MS.gene012957:CDS
GAGTGGTGAAACAACGGTAG+GGG 0.575446 3.4:-89778701 MS.gene012957:CDS
ACATCATATCCAGGTCAAGA+AGG 0.586316 3.4:-89778421 MS.gene012957:CDS
TGATAAAACATTCAGAAACA+AGG 0.589803 3.4:+89779643 None:intergenic
GATCCTTATACTGTGATGAT+GGG 0.590324 3.4:-89778541 MS.gene012957:CDS
GGCAGCATGGAAGATTCACC+TGG 0.593449 3.4:-89778653 MS.gene012957:CDS
AAGAATGATTGAACAATAGG+TGG 0.594549 3.4:+89779273 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.4:+89778745 None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG 0.597903 3.4:-89778472 MS.gene012957:CDS
TGAGTGGTGAAACAACGGTA+GGG 0.603070 3.4:-89778702 MS.gene012957:CDS
TTGGTTCAAGTCTTCGATTG+GGG 0.606286 3.4:+89779611 None:intergenic
TGCGAAATTCGGTTGAAGAG+TGG 0.613912 3.4:-89778603 MS.gene012957:CDS
GATAGAAATGGAAAAGTGTG+AGG 0.622911 3.4:+89779442 None:intergenic
TATCCAGGTCAAGAAGGAGG+AGG 0.624408 3.4:-89778415 MS.gene012957:CDS
GCTGGAAAATGATCTAGGAT+GGG 0.626677 3.4:-89778674 MS.gene012957:CDS
CGTGCACGTCATTTACAACA+TGG 0.627245 3.4:-89778817 MS.gene012957:CDS
TGCGGTGAGTGGTGAAACAA+CGG 0.630833 3.4:-89778707 MS.gene012957:CDS
GTGAGTGGTGAAACAACGGT+AGG 0.635845 3.4:-89778703 MS.gene012957:CDS
AAGACATGGTGTGTCCATCA+TGG 0.638582 3.4:+89779229 None:intergenic
TGGTTCAAGTCTTCGATTGG+GGG 0.657471 3.4:+89779612 None:intergenic
TCATATCCAGGTCAAGAAGG+AGG 0.665076 3.4:-89778418 MS.gene012957:CDS
TTGAATGGACAACAAAGACA+TGG 0.674790 3.4:+89779215 None:intergenic
AATTCCATCTATGCGGTGAG+TGG 0.681160 3.4:-89778718 MS.gene012957:CDS
ATAGAAATGGAAAAGTGTGA+GGG 0.681846 3.4:+89779443 None:intergenic
CATCATGGTTGACAGCTCTG+TGG 0.688486 3.4:+89779244 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.4:+89779418 None:intergenic
TGATGATGGGTAGCTCACCA+AGG 0.697945 3.4:-89778528 MS.gene012957:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.4:+89779420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGTATGGGAATGAATT+TGG - chr3.4:89779473-89779492 MS.gene012957:CDS 25.0%
! AATATTCTCTCTCTCAAAAT+AGG + chr3.4:89779023-89779042 None:intergenic 25.0%
! AATGTGATAAATGGCATATT+TGG + chr3.4:89778386-89778405 None:intergenic 25.0%
! ATAAACCTATTCACAATAGA+TGG - chr3.4:89778922-89778941 MS.gene012957:CDS 25.0%
! CAATAATTCAGTAACTTGAA+TGG + chr3.4:89778778-89778797 None:intergenic 25.0%
! TAATATCCCAATGTGATAAA+TGG + chr3.4:89778395-89778414 None:intergenic 25.0%
! TCAAAGAATGATTGAACAAT+AGG + chr3.4:89778708-89778727 None:intergenic 25.0%
! TGAATAGGTTTATGTGAAAT+CGG + chr3.4:89778915-89778934 None:intergenic 25.0%
! TGATAAAACATTCAGAAACA+AGG + chr3.4:89778335-89778354 None:intergenic 25.0%
! TGCAAATTATGATGATAAAG+TGG - chr3.4:89779349-89779368 MS.gene012957:CDS 25.0%
!! AAATATGCCATTTATCACAT+TGG - chr3.4:89778385-89778404 MS.gene012957:CDS 25.0%
!! AATATGCCATTTATCACATT+GGG - chr3.4:89778386-89778405 MS.gene012957:CDS 25.0%
!! CATTCAAGTTACTGAATTAT+TGG - chr3.4:89778776-89778795 MS.gene012957:CDS 25.0%
!!! TTATTGGATGGTGTTTTTAT+AGG - chr3.4:89778792-89778811 MS.gene012957:CDS 25.0%
AAGAATGATTGAACAATAGG+TGG + chr3.4:89778705-89778724 None:intergenic 30.0%
AATCTTTATCAGCACTAGTT+TGG - chr3.4:89779123-89779142 MS.gene012957:CDS 30.0%
ACAAAAACAGTGTAAGAATG+AGG + chr3.4:89778597-89778616 None:intergenic 30.0%
AGATGAAGAGTTCATGAATT+CGG - chr3.4:89779637-89779656 MS.gene012957:CDS 30.0%
ATCACCAATACAATGATTCA+TGG + chr3.4:89778823-89778842 None:intergenic 30.0%
ATCTGCATACTATATCCAAA+AGG - chr3.4:89778422-89778441 MS.gene012957:CDS 30.0%
CAAGTTACTGAATTATTGGA+TGG - chr3.4:89778780-89778799 MS.gene012957:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.4:89779242-89779261 None:intergenic 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.4:89779524-89779543 MS.gene012957:CDS 30.0%
TCTGCATACTATATCCAAAA+GGG - chr3.4:89778423-89778442 MS.gene012957:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.4:89778816-89778835 MS.gene012957:CDS 30.0%
! ATAGAAATGGAAAAGTGTGA+GGG + chr3.4:89778535-89778554 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.4:89778822-89778841 MS.gene012957:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.4:89779234-89779253 MS.gene012957:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.4:89779479-89779498 MS.gene012957:CDS 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.4:89779233-89779252 None:intergenic 35.0%
AGAGTTCATGAATTCGGTTT+CGG - chr3.4:89779643-89779662 MS.gene012957:CDS 35.0%
AGATCCATTCGATACACAAA+AGG + chr3.4:89779407-89779426 None:intergenic 35.0%
AGGTGAGAGAATATCATTGA+TGG + chr3.4:89778673-89778692 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.4:89778589-89778608 None:intergenic 35.0%
AGTGTTTCAGTTTCTCTACT+AGG + chr3.4:89778506-89778525 None:intergenic 35.0%
ATCTGAGAAAATGATGTTGC+TGG - chr3.4:89779610-89779629 MS.gene012957:CDS 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.4:89779462-89779481 MS.gene012957:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.4:89779236-89779255 None:intergenic 35.0%
GAATTGCTGGAAAATGATCT+AGG - chr3.4:89779296-89779315 MS.gene012957:CDS 35.0%
GATCCTTATACTGTGATGAT+GGG - chr3.4:89779434-89779453 MS.gene012957:CDS 35.0%
GGAAAAGTAACATCATATCC+AGG - chr3.4:89779545-89779564 MS.gene012957:CDS 35.0%
GGAAACCATCTATTGTGAAT+AGG + chr3.4:89778930-89778949 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.4:89778485-89778504 None:intergenic 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.4:89779478-89779497 MS.gene012957:CDS 35.0%
GTCATTTACAACATGGTCAA+CGG - chr3.4:89779165-89779184 MS.gene012957:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.4:89779461-89779480 MS.gene012957:CDS 35.0%
TGATAAAGTGGTGCGAAATT+CGG - chr3.4:89779361-89779380 MS.gene012957:CDS 35.0%
TGATCCTTATACTGTGATGA+TGG - chr3.4:89779433-89779452 MS.gene012957:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.4:89779467-89779486 None:intergenic 35.0%
TTGAATGGACAACAAAGACA+TGG + chr3.4:89778763-89778782 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCAT+TGG - chr3.4:89779490-89779509 MS.gene012957:CDS 35.0%
! ATTTGGTTCAAGTCTTCGAT+TGG + chr3.4:89778369-89778388 None:intergenic 35.0%
! CATCATAATTTGCAATAGCC+AGG + chr3.4:89779343-89779362 None:intergenic 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.4:89779233-89779252 MS.gene012957:CDS 35.0%
! GATAGAAATGGAAAAGTGTG+AGG + chr3.4:89778536-89778555 None:intergenic 35.0%
! GCATGAATAAATTTAGCTCC+AGG + chr3.4:89778636-89778655 None:intergenic 35.0%
! TGGTTTAGTGATGATGATGT+TGG + chr3.4:89778465-89778484 None:intergenic 35.0%
! TTCTCCTTTTGTGTATCGAA+TGG - chr3.4:89779400-89779419 MS.gene012957:CDS 35.0%
! TTTGGTTCAAGTCTTCGATT+GGG + chr3.4:89778368-89778387 None:intergenic 35.0%
!! TTGAAAGGAAGGTTGATGAT+TGG + chr3.4:89778966-89778985 None:intergenic 35.0%
!! TTTTGAAGAGAAGACCCTTT+TGG + chr3.4:89778440-89778459 None:intergenic 35.0%
!! TTTTGGGAATTCCATCTATG+CGG - chr3.4:89779250-89779269 MS.gene012957:CDS 35.0%
AACTCGTCGTGATGTTTGAA+AGG + chr3.4:89778981-89779000 None:intergenic 40.0%
ACATCATATCCAGGTCAAGA+AGG - chr3.4:89779554-89779573 MS.gene012957:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.4:89778939-89778958 MS.gene012957:CDS 40.0%
ATGATTGGACCATAACCTTC+TGG + chr3.4:89778951-89778970 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.4:89778933-89778952 MS.gene012957:CDS 40.0%
CTACCCATCATCACAGTATA+AGG + chr3.4:89779440-89779459 None:intergenic 40.0%
GGTTTATGTGAAATCGGAAC+AGG + chr3.4:89778909-89778928 None:intergenic 40.0%
TCAACGGACAACTTGCAAAT+TGG - chr3.4:89779181-89779200 MS.gene012957:CDS 40.0%
TGCTGGAAAATGATCTAGGA+TGG - chr3.4:89779300-89779319 MS.gene012957:CDS 40.0%
TTGGTTCAAGTCTTCGATTG+GGG + chr3.4:89778367-89778386 None:intergenic 40.0%
! AATGGAAAAGTGTGAGGGAA+AGG + chr3.4:89778530-89778549 None:intergenic 40.0%
! ATGGAAAAGTGTGAGGGAAA+GGG + chr3.4:89778529-89778548 None:intergenic 40.0%
! GCTGGAAAATGATCTAGGAT+GGG - chr3.4:89779301-89779320 MS.gene012957:CDS 40.0%
!! AAAGCATTGGCAGTTAGAAG+TGG - chr3.4:89779503-89779522 MS.gene012957:CDS 40.0%
!! CATTCTGGTTCGGTTGTTTA+CGG + chr3.4:89779207-89779226 None:intergenic 40.0%
!! CATTGTATTGGTGATGGAAC+TGG - chr3.4:89778828-89778847 MS.gene012957:CDS 40.0%
!! TTGGTGATGGAACTGGTTAT+TGG - chr3.4:89778835-89778854 MS.gene012957:CDS 40.0%
AAGACATGGTGTGTCCATCA+TGG + chr3.4:89778749-89778768 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.4:89779223-89779242 MS.gene012957:CDS 45.0%
AATTCCATCTATGCGGTGAG+TGG - chr3.4:89779257-89779276 MS.gene012957:CDS 45.0%
ACAATAGGTGGCACATCAAC+AGG + chr3.4:89778693-89778712 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.4:89778559-89778578 None:intergenic 45.0%
CGTAAACAACCGAACCAGAA+TGG - chr3.4:89779205-89779224 MS.gene012957:CDS 45.0%
CGTCGTGATGTTTGAAAGGA+AGG + chr3.4:89778977-89778996 None:intergenic 45.0%
CGTGCACGTCATTTACAACA+TGG - chr3.4:89779158-89779177 MS.gene012957:CDS 45.0%
GTCGATTGCAAAAACAGTCC+TGG - chr3.4:89778615-89778634 MS.gene012957:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.4:89778560-89778579 None:intergenic 45.0%
TCATATCCAGGTCAAGAAGG+AGG - chr3.4:89779557-89779576 MS.gene012957:CDS 45.0%
TGAGTGGTGAAACAACGGTA+GGG - chr3.4:89779273-89779292 MS.gene012957:CDS 45.0%
TGCGAAATTCGGTTGAAGAG+TGG - chr3.4:89779372-89779391 MS.gene012957:CDS 45.0%
TGGTTCAAGTCTTCGATTGG+GGG + chr3.4:89778366-89778385 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.4:89778543-89778562 MS.gene012957:CDS 45.0%
AACAACGGTAGGGGAATTGC+TGG - chr3.4:89779283-89779302 MS.gene012957:CDS 50.0%
AAGTCTTCGATTGGGGGACA+TGG + chr3.4:89778360-89778379 None:intergenic 50.0%
AGGAAGAGGTGGTTCCATTC+TGG + chr3.4:89779222-89779241 None:intergenic 50.0%
CACAGAGCTGTCAACCATGA+TGG - chr3.4:89778732-89778751 MS.gene012957:CDS 50.0%
CATCATGGTTGACAGCTCTG+TGG + chr3.4:89778734-89778753 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.4:89778548-89778567 None:intergenic 50.0%
GAGTGGTGAAACAACGGTAG+GGG - chr3.4:89779274-89779293 MS.gene012957:CDS 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.4:89778558-89778577 None:intergenic 50.0%
GTGAGTGGTGAAACAACGGT+AGG - chr3.4:89779272-89779291 MS.gene012957:CDS 50.0%
TATCCAGGTCAAGAAGGAGG+AGG - chr3.4:89779560-89779579 MS.gene012957:CDS 50.0%
TGCGGTGAGTGGTGAAACAA+CGG - chr3.4:89779268-89779287 MS.gene012957:CDS 50.0%
TTCACCACTCACCGCATAGA+TGG + chr3.4:89779264-89779283 None:intergenic 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.4:89779309-89779328 MS.gene012957:CDS 50.0%
! GAGGTGGTTCCATTCTGGTT+CGG + chr3.4:89779217-89779236 None:intergenic 50.0%
!! TGATGATGGGTAGCTCACCA+AGG - chr3.4:89779447-89779466 MS.gene012957:CDS 50.0%
CTTCCTCCTCCTTCTTGACC+TGG + chr3.4:89779566-89779585 None:intergenic 55.0%
GGCAGCATGGAAGATTCACC+TGG - chr3.4:89779322-89779341 MS.gene012957:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89778312 89779685 89778312 ID=MS.gene012957
chr3.4 mRNA 89778312 89779685 89778312 ID=MS.gene012957.t1;Parent=MS.gene012957
chr3.4 exon 89778312 89779685 89778312 ID=MS.gene012957.t1.exon1;Parent=MS.gene012957.t1
chr3.4 CDS 89778312 89779685 89778312 ID=cds.MS.gene012957.t1;Parent=MS.gene012957.t1
Gene Sequence

>MS.gene012957

ATGAACGCTTCTTCTCTAAACCTTGTTTCTGAATGTTTTATCAAACCATGTCCCCCAATCGAAGACTTGAACCAAATATGCCATTTATCACATTGGGATATTAGCATGTTATCTGCATACTATATCCAAAAGGGTCTTCTCTTCAAAAAACCAACATCATCATCACTAAACCAACAACATTTCATAGAGAACCTAGTAGAGAAACTGAAACACTCCCTTTCCCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTTGTCGATTGCAAAAACAGTCCTGGAGCTAAATTTATTCATGCTACTTTAGATATCACCATCAATGATATTCTCTCACCTGTTGATGTGCCACCTATTGTTCAATCATTCTTTGATCACCACAGAGCTGTCAACCATGATGGACACACCATGTCTTTGTTGTCCATTCAAGTTACTGAATTATTGGATGGTGTTTTTATAGGTTGTTCCATGAATCATTGTATTGGTGATGGAACTGGTTATTGGAATTTCTTTAACTCATTTTCTGAGATATTTCAAAATGATGTTCATGTTCCTGTTCCGATTTCACATAAACCTATTCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATCATCAACCTTCCTTTCAAACATCACGACGAGTTTATTCATAGATATGAAACACCTATTTTGAGAGAGAGAATATTTCATTTTTCAGCAGAGTCTATTGCAAAACTTAAAGCAAAAGCTAACAAAGAGTCTGATACAAACAAAATCTCTTCTTTTCAATCTTTATCAGCACTAGTTTGGAGATCTCTAACGCGTGCACGTCATTTACAACATGGTCAACGGACAACTTGCAAATTGGCCGTAAACAACCGAACCAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCCATCTATGCGGTGAGTGGTGAAACAACGGTAGGGGAATTGCTGGAAAATGATCTAGGATGGGCAGCATGGAAGATTCACCTGGCTATTGCAAATTATGATGATAAAGTGGTGCGAAATTCGGTTGAAGAGTGGTTACGTTCTCCTTTTGTGTATCGAATGGATCTGTTCTTTGATCCTTATACTGTGATGATGGGTAGCTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCAGGTCAAGAAGGAGGAGGAAGCATTGATCTTGAAGTGTGTCTTTCATCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCGGTTTCGGTGTTTAATCCTTTGTTTTAG
Protein sequence

>MS.gene012957.t1

MNASSLNLVSECFIKPCPPIEDLNQICHLSHWDISMLSAYYIQKGLLFKKPTSSSLNQQHFIENLVEKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILSPVDVPPIVQSFFDHHRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHCIGDGTGYWNFFNSFSEIFQNDVHVPVPISHKPIHNRWFPEGYGPIINLPFKHHDEFIHRYETPILRERIFHFSAESIAKLKAKANKESDTNKISSFQSLSALVWRSLTRARHLQHGQRTTCKLAVNNRTRMEPPLPKEYFGNSIYAVSGETTVGELLENDLGWAAWKIHLAIANYDDKVVRNSVEEWLRSPFVYRMDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGQEGGGSIDLEVCLSSEKMMLLETDEEFMNSVSVFNPLF