AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38699.t1 MTR_3g102020 95.186 457 22 0 1 457 1 457 0.0 905
MS.gene38699.t1 MTR_3g101960 89.013 446 48 1 1 446 1 445 0.0 840
MS.gene38699.t1 MTR_3g102010 85.249 461 59 3 1 457 2 457 0.0 811
MS.gene38699.t1 MTR_3g101970 84.292 452 57 1 1 452 1 438 0.0 802
MS.gene38699.t1 MTR_3g101900 68.653 453 136 3 1 452 2 449 0.0 637
MS.gene38699.t1 MTR_3g101930 66.520 454 147 3 1 450 2 454 0.0 625
MS.gene38699.t1 MTR_3g102030 79.874 318 38 2 87 404 29 320 0.0 524
MS.gene38699.t1 MTR_3g070170 39.837 492 217 14 2 447 8 466 2.15e-104 319
MS.gene38699.t1 MTR_4g015180 39.604 505 233 12 1 451 1 487 2.15e-99 306
MS.gene38699.t1 MTR_5g081960 39.044 502 231 12 1 447 1 482 1.30e-98 305
MS.gene38699.t1 MTR_8g041770 39.408 439 247 10 16 451 19 441 3.52e-94 291
MS.gene38699.t1 MTR_4g094842 38.646 458 252 11 4 447 2 444 3.54e-91 284
MS.gene38699.t1 MTR_4g022800 38.409 440 241 11 25 451 20 442 1.93e-89 279
MS.gene38699.t1 MTR_4g022825 38.724 439 241 10 24 451 19 440 4.56e-89 278
MS.gene38699.t1 MTR_4g022720 39.723 433 240 9 24 451 19 435 6.10e-89 278
MS.gene38699.t1 MTR_4g094835 38.605 430 242 8 25 447 28 442 4.33e-88 276
MS.gene38699.t1 MTR_0190s0020 38.318 428 247 8 28 451 28 442 1.13e-87 275
MS.gene38699.t1 MTR_4g094838 38.462 429 228 8 28 447 27 428 6.66e-87 272
MS.gene38699.t1 MTR_3g088420 38.710 434 241 10 24 451 19 433 8.59e-87 272
MS.gene38699.t1 MTR_4g022670 38.497 439 243 10 24 451 19 441 1.19e-85 270
MS.gene38699.t1 MTR_7g026860 37.358 439 245 11 24 451 19 438 1.64e-82 261
MS.gene38699.t1 MTR_3g026310 37.698 443 238 12 29 451 24 448 8.49e-81 257
MS.gene38699.t1 MTR_4g094828 38.063 444 240 14 26 452 26 451 1.04e-79 254
MS.gene38699.t1 MTR_4g022290 36.905 420 232 11 24 430 25 424 1.30e-77 249
MS.gene38699.t1 MTR_8g079710 38.771 423 223 17 25 432 25 426 2.62e-74 240
MS.gene38699.t1 MTR_4g094832 42.120 349 187 8 106 447 86 426 4.79e-73 236
MS.gene38699.t1 MTR_4g022760 36.176 387 211 11 24 387 19 392 1.23e-66 219
MS.gene38699.t1 MTR_6g045553 31.677 483 278 12 2 450 10 474 4.39e-60 204
MS.gene38699.t1 MTR_6g016815 31.090 431 212 11 24 451 33 381 1.24e-47 168
MS.gene38699.t1 MTR_8g040780 36.607 224 137 4 235 454 14 236 4.23e-40 144
MS.gene38699.t1 MTR_8g075610 27.411 394 239 14 69 441 47 414 4.81e-33 129
MS.gene38699.t1 MTR_8g096530 31.329 316 177 11 63 359 53 347 3.00e-32 128
MS.gene38699.t1 MTR_4g007540 24.837 459 288 17 6 441 3 427 1.07e-31 126
MS.gene38699.t1 MTR_2g064495 26.753 385 256 13 69 441 56 426 6.93e-31 124
MS.gene38699.t1 MTR_2g105330 26.494 385 257 13 69 441 56 426 2.63e-30 122
MS.gene38699.t1 MTR_3g098980 29.773 309 190 9 63 359 60 353 1.20e-29 120
MS.gene38699.t1 MTR_3g049150 27.233 459 252 19 22 441 25 440 1.79e-29 120
MS.gene38699.t1 MTR_7g026850 41.667 156 79 4 43 197 21 165 2.03e-29 114
MS.gene38699.t1 MTR_8g040760 35.135 222 132 5 4 224 2 212 3.00e-29 114
MS.gene38699.t1 MTR_4g127650 26.464 461 252 19 19 437 30 445 6.89e-28 115
MS.gene38699.t1 MTR_7g026770 37.853 177 89 3 156 332 132 287 9.63e-28 112
MS.gene38699.t1 MTR_3g026950 24.468 470 272 18 17 446 20 446 1.34e-27 114
MS.gene38699.t1 MTR_4g097090 26.577 444 263 16 29 437 30 445 9.64e-26 109
MS.gene38699.t1 MTR_4g094825 38.667 150 81 2 302 451 253 391 3.47e-25 107
MS.gene38699.t1 MTR_4g094825 30.453 243 138 7 29 255 92 319 4.81e-22 97.8
MS.gene38699.t1 MTR_6g053810 32.168 286 176 12 70 345 72 349 3.36e-24 105
MS.gene38699.t1 MTR_3g112180 26.812 414 242 16 14 400 11 390 1.74e-23 102
MS.gene38699.t1 MTR_7g027175 29.841 315 195 10 57 359 56 356 2.59e-23 102
MS.gene38699.t1 MTR_6g034495 43.220 118 63 3 80 194 45 161 4.68e-23 95.9
MS.gene38699.t1 MTR_5g076700 25.117 426 277 17 33 437 27 431 1.93e-22 99.8
MS.gene38699.t1 MTR_7g114470 25.532 423 280 16 33 437 28 433 6.26e-22 98.6
MS.gene38699.t1 MTR_1g081660 25.679 405 263 14 66 454 70 452 9.94e-22 97.8
MS.gene38699.t1 MTR_7g114550 26.364 440 277 20 33 448 27 443 3.74e-21 95.9
MS.gene38699.t1 MTR_3g071320 27.005 374 234 14 4 359 3 355 5.11e-21 95.5
MS.gene38699.t1 MTR_1g040195 23.944 426 281 16 48 451 16 420 6.85e-21 95.1
MS.gene38699.t1 MTR_1g040175 27.248 367 230 17 66 420 61 402 3.09e-20 93.2
MS.gene38699.t1 MTR_4g055170 27.046 281 178 8 69 335 69 336 4.78e-20 92.8
MS.gene38699.t1 MTR_3g465980 27.458 295 190 10 53 335 52 334 6.56e-20 92.4
MS.gene38699.t1 MTR_7g086000 27.020 396 237 19 72 432 63 441 1.28e-19 91.7
MS.gene38699.t1 MTR_7g114560 25.571 438 285 19 33 449 27 444 1.33e-18 88.2
MS.gene38699.t1 MTR_3g465990 26.780 295 192 10 53 335 52 334 5.15e-18 86.7
MS.gene38699.t1 MTR_6g034485 35.897 117 68 2 206 318 2 115 1.37e-17 79.3
MS.gene38699.t1 MTR_3g465930 27.899 276 177 8 69 332 69 334 1.87e-17 84.7
MS.gene38699.t1 MTR_7g114960 25.815 399 265 11 66 447 60 444 5.19e-17 83.6
MS.gene38699.t1 MTR_3g465950 28.302 265 169 7 70 322 69 324 8.09e-17 82.8
MS.gene38699.t1 MTR_7g084940 24.359 312 205 10 65 361 65 360 8.10e-17 82.8
MS.gene38699.t1 MTR_7g014160 24.788 472 285 18 18 442 19 467 9.71e-17 82.8
MS.gene38699.t1 MTR_2g015430 25.589 297 201 9 72 359 61 346 1.16e-16 82.4
MS.gene38699.t1 MTR_4g013050 23.940 401 231 16 67 441 51 403 1.33e-16 82.0
MS.gene38699.t1 MTR_1g040215 27.835 291 190 11 66 348 61 339 2.52e-16 81.3
MS.gene38699.t1 MTR_3g465910 28.674 279 167 9 70 332 69 331 5.76e-16 80.5
MS.gene38699.t1 MTR_6g015850 25.420 417 249 15 61 442 62 451 6.88e-16 80.1
MS.gene38699.t1 MTR_5g026320 24.586 423 262 17 48 446 59 448 1.89e-15 78.6
MS.gene38699.t1 MTR_6g015830 24.450 409 266 14 61 442 62 454 9.47e-15 76.6
MS.gene38699.t1 MTR_7g026830 50.000 68 33 1 112 178 28 95 2.44e-14 68.9
MS.gene38699.t1 MTR_7g056087 23.670 376 248 14 6 362 4 359 3.77e-14 74.7
MS.gene38699.t1 MTR_7g014620 24.374 439 280 21 29 442 15 426 3.97e-14 74.3
MS.gene38699.t1 MTR_1g040315 26.645 304 197 11 33 326 26 313 4.66e-14 74.3
MS.gene38699.t1 MTR_3g049140 25.980 204 133 5 243 432 27 226 7.28e-14 71.6
MS.gene38699.t1 MTR_7g085930 23.358 411 262 13 65 437 55 450 1.54e-13 72.8
MS.gene38699.t1 MTR_7g013850 23.515 421 257 15 32 403 32 436 2.03e-13 72.4
MS.gene38699.t1 MTR_7g014200 24.762 420 274 18 15 403 16 424 2.84e-13 72.0
MS.gene38699.t1 MTR_3g450710 23.151 311 176 11 63 337 59 342 2.17e-12 69.3
MS.gene38699.t1 MTR_6g015315 24.818 411 265 19 54 437 48 441 3.43e-12 68.6
MS.gene38699.t1 MTR_8g079970 24.784 347 205 14 6 331 3 314 4.56e-12 68.2
MS.gene38699.t1 MTR_7g084910 24.528 265 175 7 65 316 66 318 5.04e-12 67.8
MS.gene38699.t1 MTR_5g090830 23.779 307 187 15 64 343 61 347 7.79e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38699.t1 AT5G42830 56.885 443 182 2 13 452 14 450 0.0 527
MS.gene38699.t1 AT5G07850 54.324 451 204 2 2 451 4 453 0.0 515
MS.gene38699.t1 AT5G07860 54.000 450 197 5 4 450 6 448 3.36e-169 484
MS.gene38699.t1 AT5G07870 50.649 462 212 3 2 451 4 461 3.25e-165 474
MS.gene38699.t1 AT2G39980 40.254 472 213 9 29 451 29 480 3.50e-104 318
MS.gene38699.t1 AT5G67150 39.868 454 251 11 8 452 7 447 6.02e-101 309
MS.gene38699.t1 AT5G01210 38.900 491 238 12 1 451 1 469 1.08e-92 289
MS.gene38699.t1 AT3G50270 35.345 464 273 12 1 452 1 449 1.44e-82 262
MS.gene38699.t1 AT3G50280 34.956 452 272 11 4 447 2 439 1.64e-77 248
MS.gene38699.t1 AT3G50300 32.969 458 279 9 6 450 4 446 6.83e-75 242
MS.gene38699.t1 AT5G38130 34.932 438 259 12 6 434 24 444 1.66e-72 236
MS.gene38699.t1 AT5G67160 35.198 429 251 11 28 452 28 433 2.56e-71 232
MS.gene38699.t1 AT5G23940 33.696 460 277 11 6 449 3 450 2.67e-66 220
MS.gene38699.t1 AT3G50280 37.785 307 180 6 147 447 8 309 7.60e-56 188
MS.gene38699.t1 AT3G50290 37.838 259 148 6 148 397 15 269 1.46e-44 157
MS.gene38699.t1 AT2G19070 29.478 441 254 18 29 437 25 440 8.51e-35 135
MS.gene38699.t1 AT5G48930 25.821 457 286 16 6 441 3 427 3.02e-33 130
MS.gene38699.t1 AT5G41040 29.526 359 206 15 63 400 63 395 3.56e-32 127
MS.gene38699.t1 AT5G41040 29.526 359 206 15 63 400 79 411 5.72e-32 127
MS.gene38699.t1 AT3G48720 26.683 416 256 17 18 421 18 396 1.68e-23 102
MS.gene38699.t1 AT2G40230 27.177 379 209 13 64 410 54 397 7.65e-23 100
MS.gene38699.t1 AT5G63560 29.167 312 185 12 63 359 55 345 3.11e-22 99.0
MS.gene38699.t1 AT1G65450 26.935 323 201 12 57 359 60 367 3.27e-21 96.3
MS.gene38699.t1 AT5G57840 26.190 420 251 16 46 437 39 427 3.69e-20 92.8
MS.gene38699.t1 AT1G28680 26.351 296 199 11 72 359 65 349 3.72e-20 92.4
MS.gene38699.t1 AT1G27620 26.984 315 182 10 69 359 58 348 4.06e-20 92.8
MS.gene38699.t1 AT1G28680 26.351 296 199 11 72 359 65 349 4.97e-20 92.8
MS.gene38699.t1 AT3G26040 26.763 482 269 24 6 455 3 432 4.90e-19 89.7
MS.gene38699.t1 AT2G25150 27.667 300 185 12 62 345 60 343 6.26e-16 80.1
MS.gene38699.t1 AT5G16410 22.747 466 280 17 24 456 56 474 2.46e-15 78.6
MS.gene38699.t1 AT1G78990 24.430 307 196 11 48 343 52 333 2.54e-15 78.2
MS.gene38699.t1 AT1G03390 25.310 403 252 18 54 432 64 441 5.81e-15 77.4
MS.gene38699.t1 AT5G07080 25.448 279 172 11 70 330 71 331 6.39e-15 77.0
MS.gene38699.t1 AT1G65450 25.837 209 133 6 167 359 1 203 1.78e-13 71.2
MS.gene38699.t1 AT3G03480 25.672 335 204 15 43 356 46 356 4.50e-13 71.2
MS.gene38699.t1 AT2G23510 27.059 255 170 7 69 317 69 313 8.62e-13 70.5
MS.gene38699.t1 AT1G03940 23.239 426 266 21 57 442 54 458 2.09e-12 69.3
MS.gene38699.t1 AT3G29590 23.514 387 254 14 76 437 68 437 3.06e-12 68.9
MS.gene38699.t1 AT5G23970 24.622 463 280 19 6 447 3 417 1.23e-11 66.6

Find 103 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.3:-84441379 MS.gene38699:CDS
CATTCAAGTTACTGAATTAT+TGG 0.137906 3.3:-84441836 MS.gene38699:CDS
AATGTGATAAATGGCATATT+TGG 0.269841 3.3:+84442229 None:intergenic
AATATTCTCTCTCTCAAAAT+AGG 0.288986 3.3:+84441592 None:intergenic
AGGAAGAGGTGGTTCCATTC+TGG 0.292655 3.3:+84441393 None:intergenic
TAATATCCCAATGTGATAAA+TGG 0.297305 3.3:+84442220 None:intergenic
AATCTTTATCAGCACTAGTT+TGG 0.302845 3.3:-84441489 MS.gene38699:CDS
TCACCAAGGTTTAATATGTA+TGG 0.306646 3.3:-84441151 MS.gene38699:CDS
TCAACGGACAACTTGCAAAT+TGG 0.320331 3.3:-84441431 MS.gene38699:CDS
AATATGTATGGGAATGAATT+CGG 0.348276 3.3:-84441139 MS.gene38699:CDS
CATTCTGGTTCGGTTGTTTA+CGG 0.353477 3.3:+84441408 None:intergenic
GGAAACCATCTATTGTGAAT+AGG 0.358486 3.3:+84441685 None:intergenic
ATAAACCTATTCACAATAGA+TGG 0.359431 3.3:-84441690 MS.gene38699:CDS
TCAAAGAATGATTGAACAAT+AGG 0.361615 3.3:+84441907 None:intergenic
ATGATTGGACCATAACCTTC+TGG 0.364910 3.3:+84441664 None:intergenic
AGATGAAGAGTTCATGAATT+CGG 0.367038 3.3:-84440975 MS.gene38699:CDS
ATCTGAGAAAATGATGTTGC+TGG 0.375240 3.3:-84441002 MS.gene38699:CDS
TTCACCACTCACCGCATAGA+TGG 0.382856 3.3:+84441351 None:intergenic
TGAATAGGTTTATGTGAAAT+CGG 0.391414 3.3:+84441700 None:intergenic
TTGGTGATGGAACTGGTTAT+TGG 0.394500 3.3:-84441777 MS.gene38699:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3.3:+84441792 None:intergenic
GGATATGCTAACAAATTTGA+TGG 0.406437 3.3:-84441088 MS.gene38699:CDS
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.3:-84441673 MS.gene38699:CDS
TGATCCTTACACTGTTATGA+TGG 0.415418 3.3:-84441179 MS.gene38699:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.425196 3.3:-84442069 MS.gene38699:CDS
ACAATAGGTGGCACATCAAC+AGG 0.427451 3.3:+84441922 None:intergenic
AATATGCCATTTATCACATT+GGG 0.437912 3.3:-84442226 MS.gene38699:CDS
AGTGTTTCAGTTTCTCTACT+AGG 0.438345 3.3:+84442109 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3.3:+84442130 None:intergenic
ATTTGGTTCAAGTCTTCGAT+TGG 0.440400 3.3:+84442246 None:intergenic
TTTGGTTCAAGTCTTCGATT+GGG 0.445265 3.3:+84442247 None:intergenic
CTTCCTCCTCCTTCTTGACC+TGG 0.449239 3.3:+84441049 None:intergenic
CATTGTATTGGTGATGGAAC+TGG 0.455215 3.3:-84441784 MS.gene38699:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.3:+84442026 None:intergenic
AATGGAAAAGTGTGAGGGAA+AGG 0.463707 3.3:+84442085 None:intergenic
TTGAAAGGAAGGTTGATGAT+TGG 0.467597 3.3:+84441649 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.3:+84441379 None:intergenic
GAGGTGGTTCCATTCTGGTT+CGG 0.471982 3.3:+84441398 None:intergenic
TGATAAAGTGGTGCGAAATT+CGG 0.474906 3.3:-84441251 MS.gene38699:CDS
AGAGTTCATGAATTCGGTTT+CGG 0.476951 3.3:-84440969 MS.gene38699:CDS
GAATTCCCAAAATATTCCTT+AGG 0.479625 3.3:+84441373 None:intergenic
CATCATAATTTGCAATAGCC+AGG 0.479956 3.3:+84441272 None:intergenic
TATGGGAATGAATTCGGAAT+GGG 0.482191 3.3:-84441133 MS.gene38699:CDS
CTACCCATCATAACAGTGTA+AGG 0.483421 3.3:+84441175 None:intergenic
CAATAATTCAGTAACTTGAA+TGG 0.486762 3.3:+84441837 None:intergenic
AACAACGGTAGGGGAATTGC+TGG 0.488208 3.3:-84441329 MS.gene38699:CDS
CGTCGTGATGTTTGAAAGGA+AGG 0.488568 3.3:+84441638 None:intergenic
ATGGAAAAGTGTGAGGGAAA+GGG 0.490102 3.3:+84442086 None:intergenic
AAATATGCCATTTATCACAT+TGG 0.490158 3.3:-84442227 MS.gene38699:CDS
GGTTTATGTGAAATCGGAAC+AGG 0.490426 3.3:+84441706 None:intergenic
ATCTGCATACTATATCCAAA+AGG 0.491658 3.3:-84442190 MS.gene38699:CDS
TGTTCCATGAATCATTGTAT+TGG 0.493564 3.3:-84441796 MS.gene38699:CDS
CACCAAGGTTTAATATGTAT+GGG 0.497351 3.3:-84441150 MS.gene38699:CDS
TTCCCATACATATTAAACCT+TGG 0.501336 3.3:+84441148 None:intergenic
AGGTGAGAGAATATCATTGA+TGG 0.504727 3.3:+84441942 None:intergenic
CACAGAGCTGTTAACCATGA+TGG 0.508932 3.3:-84441880 MS.gene38699:CDS
GAATTGCTGGAAAATGATCT+AGG 0.510030 3.3:-84441316 MS.gene38699:CDS
CAAGTTACTGAATTATTGGA+TGG 0.511663 3.3:-84441832 MS.gene38699:CDS
TGCTGGAAAATGATCTAGGA+TGG 0.517189 3.3:-84441312 MS.gene38699:CDS
AGATCCATTCGATACACAAA+AGG 0.519489 3.3:+84441208 None:intergenic
TGGTTTAGTGATGATGATGT+TGG 0.530066 3.3:+84442150 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.3:-84441790 MS.gene38699:CDS
GCATGAATAAATTTAGCTCC+AGG 0.532002 3.3:+84441979 None:intergenic
GGAAAAGTAACATCTTATCC+AGG 0.542909 3.3:-84441067 MS.gene38699:CDS
AACTCGTCGTGATGTTTGAA+AGG 0.543380 3.3:+84441634 None:intergenic
CACAATAGATGGTTTCCAGA+AGG 0.548245 3.3:-84441679 MS.gene38699:CDS
CGTAAACAACCGAACCAGAA+TGG 0.549236 3.3:-84441407 MS.gene38699:CDS
GATCCTTACACTGTTATGAT+GGG 0.549602 3.3:-84441178 MS.gene38699:CDS
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.3:+84442056 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.3:+84442067 None:intergenic
TGCAAATTATGATGATAAAG+TGG 0.556314 3.3:-84441263 MS.gene38699:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.557831 3.3:-84441303 MS.gene38699:CDS
CTCATCAAAGACTAAAACAA+AGG 0.560969 3.3:+84440938 None:intergenic
TCTGCATACTATATCCAAAA+GGG 0.562084 3.3:-84442189 MS.gene38699:CDS
GTATGGGAATGAATTCGGAA+TGG 0.568846 3.3:-84441134 MS.gene38699:CDS
GTCATTTACAACATGGTCAA+CGG 0.571152 3.3:-84441447 MS.gene38699:CDS
AATGGAACCACCTCTTCCTA+AGG 0.571584 3.3:-84441389 MS.gene38699:CDS
AAGACATGGTGTGTCCATCA+TGG 0.573232 3.3:+84441866 None:intergenic
GAGTGGTGAAACAACGGTAG+GGG 0.575446 3.3:-84441338 MS.gene38699:CDS
AAAGCTTTAGCAGTTAGAAG+TGG 0.578010 3.3:-84441109 MS.gene38699:CDS
ACATCTTATCCAGGTCAAGA+AGG 0.586316 3.3:-84441058 MS.gene38699:CDS
TGATAAAACATTCAGAAACA+AGG 0.589803 3.3:+84442280 None:intergenic
GGCAGCATGGAAGATTCACC+TGG 0.593449 3.3:-84441290 MS.gene38699:CDS
AAGAATGATTGAACAATAGG+TGG 0.594549 3.3:+84441910 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.3:+84441382 None:intergenic
TGAGTGGTGAAACAACGGTA+GGG 0.603070 3.3:-84441339 MS.gene38699:CDS
TTGGTTCAAGTCTTCGATTG+GGG 0.606286 3.3:+84442248 None:intergenic
TGCGAAATTCGGTTGAAGAG+TGG 0.613912 3.3:-84441240 MS.gene38699:CDS
GATAGAAATGGAAAAGTGTG+AGG 0.622911 3.3:+84442079 None:intergenic
TATCCAGGTCAAGAAGGAGG+AGG 0.624408 3.3:-84441052 MS.gene38699:CDS
GCTGGAAAATGATCTAGGAT+GGG 0.626677 3.3:-84441311 MS.gene38699:CDS
CGTGCACGTCATTTACAACA+TGG 0.627245 3.3:-84441454 MS.gene38699:CDS
TGCGGTGAGTGGTGAAACAA+CGG 0.630833 3.3:-84441344 MS.gene38699:CDS
GTGAGTGGTGAAACAACGGT+AGG 0.635845 3.3:-84441340 MS.gene38699:CDS
TGGTTCAAGTCTTCGATTGG+GGG 0.657471 3.3:+84442249 None:intergenic
TCTTATCCAGGTCAAGAAGG+AGG 0.670221 3.3:-84441055 MS.gene38699:CDS
TTGAATGGACAACAAAGACA+TGG 0.674790 3.3:+84441852 None:intergenic
AATTCCATCTATGCGGTGAG+TGG 0.681160 3.3:-84441355 MS.gene38699:CDS
ATAGAAATGGAAAAGTGTGA+GGG 0.681846 3.3:+84442080 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.3:+84442055 None:intergenic
TTATGATGGGTAGTTCACCA+AGG 0.706961 3.3:-84441165 MS.gene38699:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.3:+84442057 None:intergenic
CATCATGGTTAACAGCTCTG+TGG 0.747659 3.3:+84441881 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGTATGGGAATGAATT+CGG - chr3.3:84442110-84442129 MS.gene38699:CDS 25.0%
! AATATTCTCTCTCTCAAAAT+AGG + chr3.3:84441660-84441679 None:intergenic 25.0%
! AATGTGATAAATGGCATATT+TGG + chr3.3:84441023-84441042 None:intergenic 25.0%
! ATAAACCTATTCACAATAGA+TGG - chr3.3:84441559-84441578 MS.gene38699:CDS 25.0%
! CAATAATTCAGTAACTTGAA+TGG + chr3.3:84441415-84441434 None:intergenic 25.0%
! TAATATCCCAATGTGATAAA+TGG + chr3.3:84441032-84441051 None:intergenic 25.0%
! TCAAAGAATGATTGAACAAT+AGG + chr3.3:84441345-84441364 None:intergenic 25.0%
! TGAATAGGTTTATGTGAAAT+CGG + chr3.3:84441552-84441571 None:intergenic 25.0%
! TGATAAAACATTCAGAAACA+AGG + chr3.3:84440972-84440991 None:intergenic 25.0%
! TGCAAATTATGATGATAAAG+TGG - chr3.3:84441986-84442005 MS.gene38699:CDS 25.0%
!! AAATATGCCATTTATCACAT+TGG - chr3.3:84441022-84441041 MS.gene38699:CDS 25.0%
!! AATATGCCATTTATCACATT+GGG - chr3.3:84441023-84441042 MS.gene38699:CDS 25.0%
!! CATTCAAGTTACTGAATTAT+TGG - chr3.3:84441413-84441432 MS.gene38699:CDS 25.0%
!!! TTATTGGATGGTGTTTTTAT+AGG - chr3.3:84441429-84441448 MS.gene38699:CDS 25.0%
AAGAATGATTGAACAATAGG+TGG + chr3.3:84441342-84441361 None:intergenic 30.0%
AATCTTTATCAGCACTAGTT+TGG - chr3.3:84441760-84441779 MS.gene38699:CDS 30.0%
ACAAAAACAGTGTAAGAATG+AGG + chr3.3:84441234-84441253 None:intergenic 30.0%
AGATGAAGAGTTCATGAATT+CGG - chr3.3:84442274-84442293 MS.gene38699:CDS 30.0%
ATCACCAATACAATGATTCA+TGG + chr3.3:84441460-84441479 None:intergenic 30.0%
ATCTGCATACTATATCCAAA+AGG - chr3.3:84441059-84441078 MS.gene38699:CDS 30.0%
CAAGTTACTGAATTATTGGA+TGG - chr3.3:84441417-84441436 MS.gene38699:CDS 30.0%
CACCAAGGTTTAATATGTAT+GGG - chr3.3:84442099-84442118 MS.gene38699:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.3:84441879-84441898 None:intergenic 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.3:84442161-84442180 MS.gene38699:CDS 30.0%
TCACCAAGGTTTAATATGTA+TGG - chr3.3:84442098-84442117 MS.gene38699:CDS 30.0%
TCTGCATACTATATCCAAAA+GGG - chr3.3:84441060-84441079 MS.gene38699:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.3:84441453-84441472 MS.gene38699:CDS 30.0%
TTCCCATACATATTAAACCT+TGG + chr3.3:84442104-84442123 None:intergenic 30.0%
! ATAGAAATGGAAAAGTGTGA+GGG + chr3.3:84441172-84441191 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.3:84441459-84441478 MS.gene38699:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.3:84441871-84441890 MS.gene38699:CDS 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.3:84441870-84441889 None:intergenic 35.0%
AGAGTTCATGAATTCGGTTT+CGG - chr3.3:84442280-84442299 MS.gene38699:CDS 35.0%
AGATCCATTCGATACACAAA+AGG + chr3.3:84442044-84442063 None:intergenic 35.0%
AGGTGAGAGAATATCATTGA+TGG + chr3.3:84441310-84441329 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.3:84441226-84441245 None:intergenic 35.0%
AGTGTTTCAGTTTCTCTACT+AGG + chr3.3:84441143-84441162 None:intergenic 35.0%
ATCTGAGAAAATGATGTTGC+TGG - chr3.3:84442247-84442266 MS.gene38699:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.3:84441873-84441892 None:intergenic 35.0%
GAATTGCTGGAAAATGATCT+AGG - chr3.3:84441933-84441952 MS.gene38699:CDS 35.0%
GATCCTTACACTGTTATGAT+GGG - chr3.3:84442071-84442090 MS.gene38699:CDS 35.0%
GGAAAAGTAACATCTTATCC+AGG - chr3.3:84442182-84442201 MS.gene38699:CDS 35.0%
GGAAACCATCTATTGTGAAT+AGG + chr3.3:84441567-84441586 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.3:84441122-84441141 None:intergenic 35.0%
GTCATTTACAACATGGTCAA+CGG - chr3.3:84441802-84441821 MS.gene38699:CDS 35.0%
TATGGGAATGAATTCGGAAT+GGG - chr3.3:84442116-84442135 MS.gene38699:CDS 35.0%
TGATAAAGTGGTGCGAAATT+CGG - chr3.3:84441998-84442017 MS.gene38699:CDS 35.0%
TGATCCTTACACTGTTATGA+TGG - chr3.3:84442070-84442089 MS.gene38699:CDS 35.0%
TTGAATGGACAACAAAGACA+TGG + chr3.3:84441400-84441419 None:intergenic 35.0%
! AAAGCTTTAGCAGTTAGAAG+TGG - chr3.3:84442140-84442159 MS.gene38699:CDS 35.0%
! ATTTGGTTCAAGTCTTCGAT+TGG + chr3.3:84441006-84441025 None:intergenic 35.0%
! CATCATAATTTGCAATAGCC+AGG + chr3.3:84441980-84441999 None:intergenic 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.3:84441870-84441889 MS.gene38699:CDS 35.0%
! GATAGAAATGGAAAAGTGTG+AGG + chr3.3:84441173-84441192 None:intergenic 35.0%
! GCATGAATAAATTTAGCTCC+AGG + chr3.3:84441273-84441292 None:intergenic 35.0%
! TGGTTTAGTGATGATGATGT+TGG + chr3.3:84441102-84441121 None:intergenic 35.0%
! TTCTCCTTTTGTGTATCGAA+TGG - chr3.3:84442037-84442056 MS.gene38699:CDS 35.0%
! TTTGGTTCAAGTCTTCGATT+GGG + chr3.3:84441005-84441024 None:intergenic 35.0%
!! TTGAAAGGAAGGTTGATGAT+TGG + chr3.3:84441603-84441622 None:intergenic 35.0%
!! TTTTGAAGAGAAGACCCTTT+TGG + chr3.3:84441077-84441096 None:intergenic 35.0%
!! TTTTGGGAATTCCATCTATG+CGG - chr3.3:84441887-84441906 MS.gene38699:CDS 35.0%
AACTCGTCGTGATGTTTGAA+AGG + chr3.3:84441618-84441637 None:intergenic 40.0%
ACATCTTATCCAGGTCAAGA+AGG - chr3.3:84442191-84442210 MS.gene38699:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.3:84441576-84441595 MS.gene38699:CDS 40.0%
ATGATTGGACCATAACCTTC+TGG + chr3.3:84441588-84441607 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.3:84441570-84441589 MS.gene38699:CDS 40.0%
CTACCCATCATAACAGTGTA+AGG + chr3.3:84442077-84442096 None:intergenic 40.0%
GGTTTATGTGAAATCGGAAC+AGG + chr3.3:84441546-84441565 None:intergenic 40.0%
GTATGGGAATGAATTCGGAA+TGG - chr3.3:84442115-84442134 MS.gene38699:CDS 40.0%
TCAACGGACAACTTGCAAAT+TGG - chr3.3:84441818-84441837 MS.gene38699:CDS 40.0%
TGCTGGAAAATGATCTAGGA+TGG - chr3.3:84441937-84441956 MS.gene38699:CDS 40.0%
TTGGTTCAAGTCTTCGATTG+GGG + chr3.3:84441004-84441023 None:intergenic 40.0%
! AATGGAAAAGTGTGAGGGAA+AGG + chr3.3:84441167-84441186 None:intergenic 40.0%
! ATGGAAAAGTGTGAGGGAAA+GGG + chr3.3:84441166-84441185 None:intergenic 40.0%
! GCTGGAAAATGATCTAGGAT+GGG - chr3.3:84441938-84441957 MS.gene38699:CDS 40.0%
!! CATTCTGGTTCGGTTGTTTA+CGG + chr3.3:84441844-84441863 None:intergenic 40.0%
!! CATTGTATTGGTGATGGAAC+TGG - chr3.3:84441465-84441484 MS.gene38699:CDS 40.0%
!! TTATGATGGGTAGTTCACCA+AGG - chr3.3:84442084-84442103 MS.gene38699:CDS 40.0%
!! TTGGTGATGGAACTGGTTAT+TGG - chr3.3:84441472-84441491 MS.gene38699:CDS 40.0%
AAGACATGGTGTGTCCATCA+TGG + chr3.3:84441386-84441405 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.3:84441860-84441879 MS.gene38699:CDS 45.0%
AATTCCATCTATGCGGTGAG+TGG - chr3.3:84441894-84441913 MS.gene38699:CDS 45.0%
ACAATAGGTGGCACATCAAC+AGG + chr3.3:84441330-84441349 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.3:84441196-84441215 None:intergenic 45.0%
CACAGAGCTGTTAACCATGA+TGG - chr3.3:84441369-84441388 MS.gene38699:CDS 45.0%
CATCATGGTTAACAGCTCTG+TGG + chr3.3:84441371-84441390 None:intergenic 45.0%
CGTAAACAACCGAACCAGAA+TGG - chr3.3:84441842-84441861 MS.gene38699:CDS 45.0%
CGTCGTGATGTTTGAAAGGA+AGG + chr3.3:84441614-84441633 None:intergenic 45.0%
CGTGCACGTCATTTACAACA+TGG - chr3.3:84441795-84441814 MS.gene38699:CDS 45.0%
GTCGATTGCAAAAACAGTCC+TGG - chr3.3:84441252-84441271 MS.gene38699:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.3:84441197-84441216 None:intergenic 45.0%
TCTTATCCAGGTCAAGAAGG+AGG - chr3.3:84442194-84442213 MS.gene38699:CDS 45.0%
TGAGTGGTGAAACAACGGTA+GGG - chr3.3:84441910-84441929 MS.gene38699:CDS 45.0%
TGCGAAATTCGGTTGAAGAG+TGG - chr3.3:84442009-84442028 MS.gene38699:CDS 45.0%
TGGTTCAAGTCTTCGATTGG+GGG + chr3.3:84441003-84441022 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.3:84441180-84441199 MS.gene38699:CDS 45.0%
AACAACGGTAGGGGAATTGC+TGG - chr3.3:84441920-84441939 MS.gene38699:CDS 50.0%
AAGTCTTCGATTGGGGGACA+TGG + chr3.3:84440997-84441016 None:intergenic 50.0%
AGGAAGAGGTGGTTCCATTC+TGG + chr3.3:84441859-84441878 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.3:84441185-84441204 None:intergenic 50.0%
GAGTGGTGAAACAACGGTAG+GGG - chr3.3:84441911-84441930 MS.gene38699:CDS 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.3:84441195-84441214 None:intergenic 50.0%
GTGAGTGGTGAAACAACGGT+AGG - chr3.3:84441909-84441928 MS.gene38699:CDS 50.0%
TATCCAGGTCAAGAAGGAGG+AGG - chr3.3:84442197-84442216 MS.gene38699:CDS 50.0%
TGCGGTGAGTGGTGAAACAA+CGG - chr3.3:84441905-84441924 MS.gene38699:CDS 50.0%
TTCACCACTCACCGCATAGA+TGG + chr3.3:84441901-84441920 None:intergenic 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.3:84441946-84441965 MS.gene38699:CDS 50.0%
! GAGGTGGTTCCATTCTGGTT+CGG + chr3.3:84441854-84441873 None:intergenic 50.0%
CTTCCTCCTCCTTCTTGACC+TGG + chr3.3:84442203-84442222 None:intergenic 55.0%
GGCAGCATGGAAGATTCACC+TGG - chr3.3:84441959-84441978 MS.gene38699:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 84440949 84442322 84440949 ID=MS.gene38699
chr3.3 mRNA 84440949 84442322 84440949 ID=MS.gene38699.t1;Parent=MS.gene38699
chr3.3 exon 84440949 84442322 84440949 ID=MS.gene38699.t1.exon1;Parent=MS.gene38699.t1
chr3.3 CDS 84440949 84442322 84440949 ID=cds.MS.gene38699.t1;Parent=MS.gene38699.t1
Gene Sequence

>MS.gene38699

ATGAACACTTCTTCTCTAAACCTTGTTTCTGAATGTTTTATCAAACCATGTCCCCCAATCGAAGACTTGAACCAAATATGCCATTTATCACATTGGGATATTAGCATGTTATCTGCATACTATATCCAAAAGGGTCTTCTCTTCAAAAAACCAACATCATCATCACTAAACCAACAACATTTCATAGAGAACCTAGTAGAGAAACTGAAACACTCCCTTTCCCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTTGTCGATTGCAAAAACAGTCCTGGAGCTAAATTTATTCATGCTACTTTAGATATCACCATCAATGATATTCTCTCACCTGTTGATGTGCCACCTATTGTTCAATCATTCTTTGATCACCACAGAGCTGTTAACCATGATGGACACACCATGTCTTTGTTGTCCATTCAAGTTACTGAATTATTGGATGGTGTTTTTATAGGTTGTTCCATGAATCATTGTATTGGTGATGGAACTGGTTATTGGAATTTCTTTAACTCATTTTCTGAGATATTTCAAAATGATGTTCATGTTCCTGTTCCGATTTCACATAAACCTATTCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATCATCAACCTTCCTTTCAAACATCACGACGAGTTTATTCATAGATATGAAACACCTATTTTGAGAGAGAGAATATTTCATTTTTCAGCAGAGTCTATTGCAAAACTTAAAGCAAAAGCTAACAAAGAGTCTGATACAAACAAAATCTCTTCTTTTCAATCTTTATCAGCACTAGTTTGGAGATCTCTAACGCGTGCACGTCATTTACAACATGGTCAACGGACAACTTGCAAATTGGCCGTAAACAACCGAACCAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCCATCTATGCGGTGAGTGGTGAAACAACGGTAGGGGAATTGCTGGAAAATGATCTAGGATGGGCAGCATGGAAGATTCACCTGGCTATTGCAAATTATGATGATAAAGTGGTGCGAAATTCGGTTGAAGAGTGGTTACGTTCTCCTTTTGTGTATCGAATGGATCTGTTTTTTGATCCTTACACTGTTATGATGGGTAGTTCACCAAGGTTTAATATGTATGGGAATGAATTCGGAATGGGAAAAGCTTTAGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCTTATCCAGGTCAAGAAGGAGGAGGAAGCATTGATCTTGAAGTGTGTCTTTCATCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCGGTTTCGGTGTTTAATCCTTTGTTTTAG
Protein sequence

>MS.gene38699.t1

MNTSSLNLVSECFIKPCPPIEDLNQICHLSHWDISMLSAYYIQKGLLFKKPTSSSLNQQHFIENLVEKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILSPVDVPPIVQSFFDHHRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHCIGDGTGYWNFFNSFSEIFQNDVHVPVPISHKPIHNRWFPEGYGPIINLPFKHHDEFIHRYETPILRERIFHFSAESIAKLKAKANKESDTNKISSFQSLSALVWRSLTRARHLQHGQRTTCKLAVNNRTRMEPPLPKEYFGNSIYAVSGETTVGELLENDLGWAAWKIHLAIANYDDKVVRNSVEEWLRSPFVYRMDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGQEGGGSIDLEVCLSSEKMMLLETDEEFMNSVSVFNPLF