AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95242

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95242.t1 MTR_3g101970 89.823 452 31 2 1 451 1 438 0.0 842
MS.gene95242.t1 MTR_3g101960 89.663 445 46 0 1 445 1 445 0.0 839
MS.gene95242.t1 MTR_3g102020 84.902 457 68 1 1 456 1 457 0.0 813
MS.gene95242.t1 MTR_3g102010 79.565 460 86 2 1 456 2 457 0.0 760
MS.gene95242.t1 MTR_3g101900 66.813 455 140 4 1 451 2 449 0.0 614
MS.gene95242.t1 MTR_3g101930 66.000 450 139 5 9 449 10 454 0.0 603
MS.gene95242.t1 MTR_3g102030 72.327 318 62 2 86 403 29 320 4.00e-167 473
MS.gene95242.t1 MTR_4g015180 39.286 504 235 11 1 450 1 487 1.83e-97 301
MS.gene95242.t1 MTR_3g070170 38.272 486 226 13 5 446 11 466 2.59e-97 300
MS.gene95242.t1 MTR_5g081960 38.124 501 236 12 1 446 1 482 1.33e-93 291
MS.gene95242.t1 MTR_4g094842 38.210 458 255 11 3 446 1 444 7.37e-92 286
MS.gene95242.t1 MTR_0190s0020 38.642 427 246 8 28 450 28 442 1.68e-89 280
MS.gene95242.t1 MTR_8g041770 38.824 425 244 9 29 450 30 441 5.24e-88 275
MS.gene95242.t1 MTR_4g022720 39.080 435 245 9 21 450 16 435 3.45e-86 271
MS.gene95242.t1 MTR_4g022800 37.472 443 248 11 21 450 16 442 8.10e-86 270
MS.gene95242.t1 MTR_4g022825 37.330 442 250 9 20 450 15 440 3.23e-85 268
MS.gene95242.t1 MTR_4g094835 36.324 457 263 10 3 446 1 442 1.93e-83 264
MS.gene95242.t1 MTR_4g022670 36.961 441 252 10 21 450 16 441 4.83e-82 260
MS.gene95242.t1 MTR_7g026860 36.878 442 248 11 21 450 16 438 1.15e-81 259
MS.gene95242.t1 MTR_3g088420 37.500 432 250 9 23 450 18 433 2.29e-81 258
MS.gene95242.t1 MTR_4g094838 36.682 428 236 8 28 446 27 428 7.76e-80 254
MS.gene95242.t1 MTR_4g094828 36.285 463 259 14 8 451 6 451 5.75e-79 252
MS.gene95242.t1 MTR_3g026310 37.273 440 243 11 29 450 24 448 1.86e-78 251
MS.gene95242.t1 MTR_4g022290 35.952 420 237 10 23 429 24 424 6.78e-76 244
MS.gene95242.t1 MTR_8g079710 36.047 430 232 14 21 431 21 426 1.82e-69 228
MS.gene95242.t1 MTR_4g094832 38.329 347 203 5 105 446 86 426 4.11e-67 221
MS.gene95242.t1 MTR_4g022760 32.653 441 227 10 21 450 16 397 8.51e-66 216
MS.gene95242.t1 MTR_6g045553 30.785 484 274 12 5 449 13 474 1.67e-59 202
MS.gene95242.t1 MTR_6g016815 30.698 430 216 12 23 450 32 381 6.21e-48 169
MS.gene95242.t1 MTR_8g040780 36.697 218 133 4 240 453 20 236 3.20e-40 145
MS.gene95242.t1 MTR_4g007540 25.607 453 291 16 6 440 3 427 3.37e-32 127
MS.gene95242.t1 MTR_8g075610 27.595 395 237 14 68 440 47 414 5.37e-32 127
MS.gene95242.t1 MTR_2g064495 26.044 407 273 13 48 440 34 426 8.47e-31 123
MS.gene95242.t1 MTR_2g105330 25.799 407 274 13 48 440 34 426 1.93e-30 122
MS.gene95242.t1 MTR_7g026850 44.000 150 73 4 42 190 20 159 4.97e-30 115
MS.gene95242.t1 MTR_8g040760 35.945 217 128 6 8 223 6 212 6.92e-29 113
MS.gene95242.t1 MTR_8g096530 30.124 322 180 13 62 362 53 350 4.71e-28 116
MS.gene95242.t1 MTR_7g026770 37.288 177 90 3 155 331 132 287 3.17e-26 108
MS.gene95242.t1 MTR_3g098980 29.545 308 187 12 68 362 66 356 9.89e-26 109
MS.gene95242.t1 MTR_4g127650 27.069 447 256 18 19 431 30 440 1.07e-25 109
MS.gene95242.t1 MTR_3g049150 26.521 411 240 16 56 431 46 429 7.60e-25 107
MS.gene95242.t1 MTR_4g094825 33.918 171 102 2 280 450 232 391 3.37e-24 104
MS.gene95242.t1 MTR_4g094825 33.333 195 113 6 29 220 92 272 7.38e-21 94.4
MS.gene95242.t1 MTR_6g053810 30.986 284 182 10 69 344 72 349 7.61e-24 103
MS.gene95242.t1 MTR_6g034495 44.068 118 62 3 79 193 45 161 3.00e-23 96.3
MS.gene95242.t1 MTR_4g097090 25.221 452 266 17 29 440 30 449 7.04e-23 101
MS.gene95242.t1 MTR_5g076700 24.945 457 293 17 2 436 3 431 7.13e-23 101
MS.gene95242.t1 MTR_3g112180 29.143 350 216 13 65 399 58 390 2.05e-22 99.8
MS.gene95242.t1 MTR_3g026950 24.752 404 252 14 62 431 48 433 1.35e-21 97.4
MS.gene95242.t1 MTR_1g040175 28.338 367 226 17 65 419 61 402 1.57e-21 97.1
MS.gene95242.t1 MTR_7g114470 25.397 441 281 18 33 447 28 446 1.07e-20 94.7
MS.gene95242.t1 MTR_3g465980 26.301 346 217 13 6 334 10 334 2.77e-20 93.6
MS.gene95242.t1 MTR_1g040195 25.459 436 254 19 50 450 21 420 5.16e-20 92.4
MS.gene95242.t1 MTR_7g086000 26.835 395 243 17 71 433 63 443 1.46e-19 91.3
MS.gene95242.t1 MTR_1g081660 26.667 390 247 15 65 437 70 437 2.04e-19 90.9
MS.gene95242.t1 MTR_4g055170 25.623 281 182 8 68 334 69 336 2.65e-19 90.5
MS.gene95242.t1 MTR_7g114960 26.010 396 268 10 65 446 60 444 3.29e-19 90.1
MS.gene95242.t1 MTR_7g027175 28.272 382 233 15 56 420 56 413 3.65e-19 89.7
MS.gene95242.t1 MTR_6g015320 25.980 408 249 15 60 436 58 443 4.42e-18 86.7
MS.gene95242.t1 MTR_3g465990 25.723 346 219 13 6 334 10 334 6.53e-18 86.3
MS.gene95242.t1 MTR_7g114520 25.519 482 286 20 2 448 4 447 6.99e-18 86.3
MS.gene95242.t1 MTR_7g014160 24.786 468 295 20 15 441 16 467 1.20e-17 85.5
MS.gene95242.t1 MTR_7g084940 25.397 315 197 11 64 363 65 356 2.30e-17 84.3
MS.gene95242.t1 MTR_1g040315 27.687 307 195 11 30 325 23 313 2.49e-17 84.3
MS.gene95242.t1 MTR_6g034485 35.088 114 73 1 205 317 2 115 4.45e-17 77.8
MS.gene95242.t1 MTR_3g465930 27.437 277 175 9 69 331 70 334 5.93e-16 80.1
MS.gene95242.t1 MTR_2g015430 24.916 297 203 9 71 358 61 346 2.27e-15 78.6
MS.gene95242.t1 MTR_4g013050 23.678 397 229 16 66 436 51 399 2.55e-15 78.2
MS.gene95242.t1 MTR_7g013850 24.594 431 243 17 30 402 30 436 2.97e-15 78.2
MS.gene95242.t1 MTR_7g026830 51.429 70 33 1 111 179 28 97 3.26e-15 71.6
MS.gene95242.t1 MTR_3g049140 26.108 203 134 4 242 431 27 226 1.48e-14 73.9
MS.gene95242.t1 MTR_3g465910 26.481 287 163 9 69 331 69 331 3.27e-14 75.1
MS.gene95242.t1 MTR_6g015315 25.547 411 264 14 55 439 50 444 4.68e-14 74.3
MS.gene95242.t1 MTR_3g465950 26.909 275 180 7 69 331 69 334 5.87e-14 73.9
MS.gene95242.t1 MTR_7g014620 23.543 429 277 20 29 432 15 417 1.13e-13 73.2
MS.gene95242.t1 MTR_5g090880 24.054 370 229 16 64 399 63 414 5.48e-13 71.2
MS.gene95242.t1 MTR_7g085930 24.490 343 210 12 50 367 45 363 1.19e-12 70.1
MS.gene95242.t1 MTR_7g014610 22.173 451 293 19 29 436 29 464 3.16e-12 68.9
MS.gene95242.t1 MTR_2g089755 22.384 411 268 13 29 402 37 433 9.54e-12 67.4
MS.gene95242.t1 MTR_2g045540 34.091 88 50 1 242 329 27 106 9.73e-12 62.0
MS.gene95242.t1 MTR_0502s0010 28.571 252 132 12 111 337 2 230 1.88e-11 64.3
MS.gene95242.t1 MTR_7g014630 30.233 215 136 7 2 209 3 210 3.07e-11 65.1
MS.gene95242.t1 MTR_1g094790 25.610 328 209 11 6 320 3 308 6.68e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95242.t1 AT5G42830 56.054 446 180 4 13 451 14 450 0.0 513
MS.gene95242.t1 AT5G07850 54.036 446 204 1 6 450 8 453 2.43e-175 499
MS.gene95242.t1 AT5G07860 53.348 448 198 5 6 449 8 448 1.44e-162 467
MS.gene95242.t1 AT5G07870 50.437 458 210 3 6 450 8 461 1.11e-159 460
MS.gene95242.t1 AT5G67150 40.265 452 251 11 8 451 7 447 4.67e-102 312
MS.gene95242.t1 AT2G39980 40.127 471 214 10 29 450 29 480 1.90e-99 306
MS.gene95242.t1 AT5G01210 38.525 488 241 12 3 450 1 469 6.79e-89 279
MS.gene95242.t1 AT3G50270 36.564 454 266 11 8 451 8 449 1.59e-83 264
MS.gene95242.t1 AT3G50280 35.604 455 266 13 3 446 1 439 1.70e-79 254
MS.gene95242.t1 AT3G50300 35.388 438 262 10 3 432 1 425 1.10e-75 244
MS.gene95242.t1 AT5G38130 35.909 440 249 14 6 432 24 443 6.24e-71 232
MS.gene95242.t1 AT5G67160 34.813 428 253 11 28 451 28 433 1.17e-68 225
MS.gene95242.t1 AT5G23940 33.043 460 279 12 6 448 3 450 2.50e-63 213
MS.gene95242.t1 AT3G50280 38.141 312 176 8 144 446 6 309 7.58e-56 188
MS.gene95242.t1 AT3G50290 37.838 259 148 6 147 396 15 269 3.68e-42 150
MS.gene95242.t1 AT5G48930 25.877 456 286 16 6 440 3 427 4.75e-32 127
MS.gene95242.t1 AT2G19070 27.397 438 256 18 29 431 25 435 6.61e-30 121
MS.gene95242.t1 AT5G41040 28.771 358 210 14 62 399 63 395 3.34e-27 113
MS.gene95242.t1 AT5G41040 28.771 358 210 14 62 399 79 411 4.90e-27 113
MS.gene95242.t1 AT3G48720 27.336 428 237 21 18 420 18 396 1.31e-22 100
MS.gene95242.t1 AT1G65450 28.333 300 180 12 56 335 60 344 1.33e-21 97.4
MS.gene95242.t1 AT5G63560 26.786 392 237 16 68 440 61 421 5.33e-20 92.4
MS.gene95242.t1 AT2G40230 26.667 375 216 13 63 409 54 397 9.73e-20 91.7
MS.gene95242.t1 AT1G31490 27.273 418 250 21 58 455 52 435 8.23e-19 89.0
MS.gene95242.t1 AT1G28680 25.503 298 199 11 71 358 65 349 6.96e-18 85.9
MS.gene95242.t1 AT1G27620 28.155 309 186 10 68 358 58 348 8.82e-18 85.9
MS.gene95242.t1 AT1G28680 25.503 298 199 11 71 358 65 349 1.04e-17 85.5
MS.gene95242.t1 AT3G26040 25.941 478 277 22 6 454 3 432 1.13e-17 85.5
MS.gene95242.t1 AT1G03390 24.691 405 246 18 56 431 67 441 4.19e-16 80.9
MS.gene95242.t1 AT1G78990 22.977 309 197 12 48 342 52 333 5.51e-16 80.5
MS.gene95242.t1 AT5G57840 24.236 458 287 18 6 436 3 427 1.77e-15 78.6
MS.gene95242.t1 AT2G25150 26.667 300 188 12 61 344 60 343 2.79e-15 78.2
MS.gene95242.t1 AT5G16410 24.402 418 256 17 66 455 89 474 1.22e-14 76.3
MS.gene95242.t1 AT3G03480 25.915 328 211 13 30 342 33 343 1.61e-14 75.9
MS.gene95242.t1 AT1G24420 26.407 462 298 19 6 456 5 435 1.33e-13 72.8
MS.gene95242.t1 AT1G24420 26.407 462 298 19 6 456 5 435 1.33e-13 72.8
MS.gene95242.t1 AT3G29590 23.177 384 259 13 75 436 68 437 6.94e-13 70.9
MS.gene95242.t1 AT5G39080 21.339 478 309 15 3 439 2 453 4.65e-11 65.1

Find 108 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.2:-6781429 MS.gene95242:CDS
CAAAATGATATTCCTGTTTA+TGG 0.111562 3.2:-6781774 MS.gene95242:CDS
TACGCACCACTTTATAATTA+TGG 0.182461 3.2:+6781307 None:intergenic
AATACGTATGGAAATGAATT+TGG 0.183687 3.2:-6781189 MS.gene95242:CDS
TTTGCGATGGAACATCTTAT+TGG 0.230790 3.2:-6781827 MS.gene95242:CDS
TTAAATGGAAGGTTGATAAT+TGG 0.249266 3.2:+6781699 None:intergenic
AACTCGTGGTGATGTTTAAA+TGG 0.296206 3.2:+6781684 None:intergenic
AATCTTTATCAGCATTAGTT+TGG 0.307683 3.2:-6781539 MS.gene95242:CDS
CCACTTATCATCACAGTATA+AGG 0.318537 3.2:+6781225 None:intergenic
CACGAGTTTGTTCACACATA+TGG 0.341216 3.2:-6781669 MS.gene95242:CDS
AATGTGTTAAATGACATATT+TGG 0.351013 3.2:+6782276 None:intergenic
ATAATTGGACCATAACCTTC+TGG 0.355852 3.2:+6781714 None:intergenic
AACATGCAGAAACGATGTTT+AGG 0.378660 3.2:+6782333 None:intergenic
ACAATGGGTGGGACATCAAT+GGG 0.389297 3.2:+6781972 None:intergenic
TCAGAGAACAACTTGCAAAA+TGG 0.401348 3.2:-6781481 MS.gene95242:CDS
GGATATGCTAACAAATTTGA+TGG 0.406437 3.2:-6781138 MS.gene95242:CDS
CTCCCTCCTCCTTCTTGACC+TGG 0.408040 3.2:+6781099 None:intergenic
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.2:-6781723 MS.gene95242:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.415536 3.2:-6782119 MS.gene95242:CDS
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.2:+6781749 None:intergenic
ATCTACACATTATAACCAAA+AGG 0.424756 3.2:-6782237 MS.gene95242:CDS
ATTTGGAATGGGAAAAGCTT+TGG 0.427618 3.2:-6781172 MS.gene95242:CDS
ATCGCAAAGACAATGATTCA+TGG 0.428075 3.2:+6781842 None:intergenic
AATATGTCATTTAACACATT+GGG 0.429631 3.2:-6782273 MS.gene95242:CDS
ACATGCAGAAACGATGTTTA+GGG 0.431483 3.2:+6782334 None:intergenic
ATCAACCTATCCACAATAGA+TGG 0.431811 3.2:-6781740 MS.gene95242:CDS
ATTTGGTTTGAGTCTTCGAT+TGG 0.434962 3.2:+6782293 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3.2:+6782180 None:intergenic
TGTGAAATGGGACCATAAAC+AGG 0.441469 3.2:+6781762 None:intergenic
TTTGGTTTGAGTCTTCGATT+GGG 0.443458 3.2:+6782294 None:intergenic
TGCATGAATAAATTTAGCTC+CGG 0.444458 3.2:+6782028 None:intergenic
AATGGAAAAGCGTGAGGGAA+AGG 0.449937 3.2:+6782135 None:intergenic
AACGACGGTAGAGGAATTGC+TGG 0.453571 3.2:-6781379 MS.gene95242:CDS
AGTATAAGAATGAGGATCTT+CGG 0.458409 3.2:+6782076 None:intergenic
TATGGAAATGAATTTGGAAT+GGG 0.460328 3.2:-6781183 MS.gene95242:CDS
ATGGAAAAGCGTGAGGGAAA+GGG 0.461186 3.2:+6782136 None:intergenic
GACAATGGGTGGGACATCAA+TGG 0.465845 3.2:+6781971 None:intergenic
AGTGTTTCAGTTTGTCCACT+AGG 0.466815 3.2:+6782159 None:intergenic
GAGGTGGTTCCATTCTTGTT+CGG 0.467336 3.2:+6781448 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.2:+6781429 None:intergenic
CACAAAGCAGTCAGCCATGA+TGG 0.473653 3.2:-6781930 MS.gene95242:CDS
CAAGTCACAGAATTAGTAGA+TGG 0.474789 3.2:-6781882 MS.gene95242:CDS
GAATTGCTGGAGAATGATCT+AGG 0.477520 3.2:-6781366 MS.gene95242:CDS
GAATTCCCAAAATATTCCTT+AGG 0.477642 3.2:+6781423 None:intergenic
TCAAAGAACGATTGGACAAT+GGG 0.480071 3.2:+6781957 None:intergenic
GGAAAAGTAATTTCATATCC+AGG 0.482689 3.2:-6781117 MS.gene95242:CDS
AAATCTGTTCGATACACAAA+TGG 0.488612 3.2:+6781258 None:intergenic
TCACCGAGGTTTAATACGTA+TGG 0.489403 3.2:-6781201 MS.gene95242:CDS
AAATATGTCATTTAACACAT+TGG 0.493987 3.2:-6782274 MS.gene95242:CDS
TGCGTAGGTCGGTTGAAGAA+TGG 0.503382 3.2:-6781290 MS.gene95242:CDS
TGCAGTGAGTGCTGAAACGA+CGG 0.506205 3.2:-6781394 MS.gene95242:CDS
TGTTGGTTTAGTAATGATGT+TGG 0.510991 3.2:+6782197 None:intergenic
CAATGGGTGGGACATCAATG+GGG 0.513002 3.2:+6781973 None:intergenic
ACCTGAGAAAATGATGGTGC+TGG 0.523005 3.2:-6781052 MS.gene95242:CDS
TGCTGGAGAATGATCTAGGA+TGG 0.523136 3.2:-6781362 MS.gene95242:CDS
TACAAACAACCGAACAAGAA+TGG 0.524034 3.2:-6781457 MS.gene95242:CDS
GCATGAATAAATTTAGCTCC+GGG 0.529301 3.2:+6782029 None:intergenic
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.2:+6781750 None:intergenic
GTATGGAAATGAATTTGGAA+TGG 0.534748 3.2:-6781184 MS.gene95242:CDS
CCTTATACTGTGATGATAAG+TGG 0.535234 3.2:-6781225 MS.gene95242:CDS
TGTGATGGTCAAAGAACGAT+TGG 0.541390 3.2:+6781949 None:intergenic
AATGGAACCACCTCTTCCTA+AGG 0.542226 3.2:-6781439 MS.gene95242:CDS
TGTAAACCATAATTATAAAG+TGG 0.543456 3.2:-6781313 MS.gene95242:CDS
ATGAATCATTGTCTTTGCGA+TGG 0.545555 3.2:-6781840 MS.gene95242:CDS
TCTACACATTATAACCAAAA+GGG 0.546861 3.2:-6782236 MS.gene95242:CDS
GGAAACCATCTATTGTGGAT+AGG 0.548035 3.2:+6781735 None:intergenic
CGTGGTGATGTTTAAATGGA+AGG 0.548108 3.2:+6781688 None:intergenic
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.2:+6782106 None:intergenic
GAGTGCTGAAACGACGGTAG+AGG 0.552736 3.2:-6781388 MS.gene95242:CDS
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.2:+6782117 None:intergenic
AAAGCTTTGGCAGTTAGAAG+TGG 0.567341 3.2:-6781159 MS.gene95242:CDS
AATGGGTGGGACATCAATGG+GGG 0.568371 3.2:+6781974 None:intergenic
TTGGTTTGAGTCTTCGATTG+GGG 0.568932 3.2:+6782295 None:intergenic
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.2:-6781353 MS.gene95242:CDS
GTCAAAGAACGATTGGACAA+TGG 0.570569 3.2:+6781956 None:intergenic
ACGAAAACAGTATAAGAATG+AGG 0.571532 3.2:+6782068 None:intergenic
CATGGCTGACTGCTTTGTGA+TGG 0.574372 3.2:+6781934 None:intergenic
TATAGCGAAACTTAAAGCAA+AGG 0.578915 3.2:-6781601 MS.gene95242:CDS
TATCCAGGTCAAGAAGGAGG+AGG 0.584517 3.2:-6781102 MS.gene95242:CDS
CACAATAGATGGTTTCCAGA+AGG 0.584706 3.2:-6781729 MS.gene95242:CDS
ATTTCATATCCAGGTCAAGA+AGG 0.586316 3.2:-6781108 MS.gene95242:CDS
CAGAAACGATGTTTAGGGAA+GGG 0.587217 3.2:+6782339 None:intergenic
GTTATAATGTGTAGATAACA+TGG 0.589863 3.2:+6782244 None:intergenic
ATAGTCAAAGATTAGAACAA+AGG 0.594150 3.2:+6780988 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.2:+6781432 None:intergenic
CTTCTGGAAACCATCTATTG+TGG 0.595445 3.2:+6781730 None:intergenic
TTATAAAGTGGTGCGTAGGT+CGG 0.595845 3.2:-6781301 MS.gene95242:CDS
ATCCAGGTCAAGAAGGAGGA+GGG 0.602246 3.2:-6781101 MS.gene95242:CDS
GCAGAAACGATGTTTAGGGA+AGG 0.605789 3.2:+6782338 None:intergenic
TTTCCATACGTATTAAACCT+CGG 0.609189 3.2:+6781198 None:intergenic
AAGAACGATTGGACAATGGG+TGG 0.614907 3.2:+6781960 None:intergenic
ATAATTATAAAGTGGTGCGT+AGG 0.616143 3.2:-6781305 MS.gene95242:CDS
AGAACGATTGGACAATGGGT+GGG 0.617288 3.2:+6781961 None:intergenic
GCTGGAGAATGATCTAGGAT+GGG 0.628974 3.2:-6781361 MS.gene95242:CDS
AAGACATGGTGTGTCCATCA+TGG 0.634174 3.2:+6781916 None:intergenic
TGGTTTGAGTCTTCGATTGG+GGG 0.638810 3.2:+6782296 None:intergenic
GATAGAAATGGAAAAGCGTG+AGG 0.641029 3.2:+6782129 None:intergenic
CATGCACGTCGACTACAACA+TGG 0.651574 3.2:-6781504 MS.gene95242:CDS
TCTTTCACCTGAGAAAATGA+TGG 0.654677 3.2:-6781058 MS.gene95242:CDS
TCATATCCAGGTCAAGAAGG+AGG 0.656740 3.2:-6781105 MS.gene95242:CDS
ATAGAAATGGAAAAGCGTGA+GGG 0.687050 3.2:+6782130 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.2:+6782105 None:intergenic
GGCAGCATGGAAAATTCATG+TGG 0.705154 3.2:-6781340 MS.gene95242:CDS
TTGAATAGATAACAAAGACA+TGG 0.705301 3.2:+6781902 None:intergenic
ACATTTCATAGAGAACCTAG+TGG 0.711661 3.2:-6782174 MS.gene95242:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.2:+6782107 None:intergenic
CATATGTGTGAACAAACTCG+TGG 0.741219 3.2:+6781670 None:intergenic
TGATGATAAGTGGTTCACCG+AGG 0.741768 3.2:-6781215 MS.gene95242:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTGTTAAATGACATATT+TGG + chr3.2:6781073-6781092 None:intergenic 20.0%
!! TGTAAACCATAATTATAAAG+TGG - chr3.2:6782033-6782052 MS.gene95242:CDS 20.0%
!!! AAATATGTCATTTAACACAT+TGG - chr3.2:6781072-6781091 MS.gene95242:CDS 20.0%
!!! AAGATTCTCTCTTTTAAAAT+GGG + chr3.2:6781707-6781726 None:intergenic 20.0%
!!! AATATGTCATTTAACACATT+GGG - chr3.2:6781073-6781092 MS.gene95242:CDS 20.0%
! AATACGTATGGAAATGAATT+TGG - chr3.2:6782157-6782176 MS.gene95242:CDS 25.0%
! AATCTTTATCAGCATTAGTT+TGG - chr3.2:6781807-6781826 MS.gene95242:CDS 25.0%
! ATCTACACATTATAACCAAA+AGG - chr3.2:6781109-6781128 MS.gene95242:CDS 25.0%
! CAAAATGATATTCCTGTTTA+TGG - chr3.2:6781572-6781591 MS.gene95242:CDS 25.0%
! GTTATAATGTGTAGATAACA+TGG + chr3.2:6781105-6781124 None:intergenic 25.0%
! TCTACACATTATAACCAAAA+GGG - chr3.2:6781110-6781129 MS.gene95242:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.2:6781447-6781466 None:intergenic 25.0%
!! TATGGAAATGAATTTGGAAT+GGG - chr3.2:6782163-6782182 MS.gene95242:CDS 25.0%
!!! GAAGATTCTCTCTTTTAAAA+TGG + chr3.2:6781708-6781727 None:intergenic 25.0%
!!! TTAAATGGAAGGTTGATAAT+TGG + chr3.2:6781650-6781669 None:intergenic 25.0%
AAATCTGTTCGATACACAAA+TGG + chr3.2:6782091-6782110 None:intergenic 30.0%
ACGAAAACAGTATAAGAATG+AGG + chr3.2:6781281-6781300 None:intergenic 30.0%
AGTATAAGAATGAGGATCTT+CGG + chr3.2:6781273-6781292 None:intergenic 30.0%
ATAATTATAAAGTGGTGCGT+AGG - chr3.2:6782041-6782060 MS.gene95242:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.2:6781926-6781945 None:intergenic 30.0%
GGAAAAGTAATTTCATATCC+AGG - chr3.2:6782229-6782248 MS.gene95242:CDS 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.2:6782208-6782227 MS.gene95242:CDS 30.0%
GTATGGAAATGAATTTGGAA+TGG - chr3.2:6782162-6782181 MS.gene95242:CDS 30.0%
TACGCACCACTTTATAATTA+TGG + chr3.2:6782042-6782061 None:intergenic 30.0%
TATAGCGAAACTTAAAGCAA+AGG - chr3.2:6781745-6781764 MS.gene95242:CDS 30.0%
TTTCCATACGTATTAAACCT+CGG + chr3.2:6782151-6782170 None:intergenic 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.2:6781918-6781937 MS.gene95242:CDS 30.0%
! TGCATGAATAAATTTAGCTC+CGG + chr3.2:6781321-6781340 None:intergenic 30.0%
! TTAGTAGATGGTGTTTTCAT+TGG - chr3.2:6781476-6781495 MS.gene95242:CDS 30.0%
!! TGTTGGTTTAGTAATGATGT+TGG + chr3.2:6781152-6781171 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.2:6781917-6781936 None:intergenic 35.0%
AACATGCAGAAACGATGTTT+AGG + chr3.2:6781016-6781035 None:intergenic 35.0%
ACATGCAGAAACGATGTTTA+GGG + chr3.2:6781015-6781034 None:intergenic 35.0%
ACATTTCATAGAGAACCTAG+TGG - chr3.2:6781172-6781191 MS.gene95242:CDS 35.0%
ATAATTGGACCATAACCTTC+TGG + chr3.2:6781635-6781654 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.2:6781606-6781625 MS.gene95242:CDS 35.0%
ATCGCAAAGACAATGATTCA+TGG + chr3.2:6781507-6781526 None:intergenic 35.0%
ATGAATCATTGTCTTTGCGA+TGG - chr3.2:6781506-6781525 MS.gene95242:CDS 35.0%
ATTTCATATCCAGGTCAAGA+AGG - chr3.2:6782238-6782257 MS.gene95242:CDS 35.0%
CAAGTCACAGAATTAGTAGA+TGG - chr3.2:6781464-6781483 MS.gene95242:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.2:6781920-6781939 None:intergenic 35.0%
CCACTTATCATCACAGTATA+AGG + chr3.2:6782124-6782143 None:intergenic 35.0%
CCTTATACTGTGATGATAAG+TGG - chr3.2:6782121-6782140 MS.gene95242:CDS 35.0%
CTATAGATTCCGCTGAAAAA+TGG + chr3.2:6781730-6781749 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.2:6781169-6781188 None:intergenic 35.0%
TACAAACAACCGAACAAGAA+TGG - chr3.2:6781889-6781908 MS.gene95242:CDS 35.0%
TCAAAGAACGATTGGACAAT+GGG + chr3.2:6781392-6781411 None:intergenic 35.0%
TCAGAGAACAACTTGCAAAA+TGG - chr3.2:6781865-6781884 MS.gene95242:CDS 35.0%
TCTTTCACCTGAGAAAATGA+TGG - chr3.2:6782288-6782307 MS.gene95242:CDS 35.0%
TTTGCGATGGAACATCTTAT+TGG - chr3.2:6781519-6781538 MS.gene95242:CDS 35.0%
! AACTCGTGGTGATGTTTAAA+TGG + chr3.2:6781665-6781684 None:intergenic 35.0%
! ATAGAAATGGAAAAGCGTGA+GGG + chr3.2:6781219-6781238 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCTT+TGG - chr3.2:6782174-6782193 MS.gene95242:CDS 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.2:6781917-6781936 MS.gene95242:CDS 35.0%
! GCATGAATAAATTTAGCTCC+GGG + chr3.2:6781320-6781339 None:intergenic 35.0%
!! ATTTGGTTTGAGTCTTCGAT+TGG + chr3.2:6781056-6781075 None:intergenic 35.0%
!! GAGAATCTTCCATTTTTCAG+CGG - chr3.2:6781718-6781737 MS.gene95242:CDS 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.2:6781599-6781618 None:intergenic 35.0%
!! TTTGGTTTGAGTCTTCGATT+GGG + chr3.2:6781055-6781074 None:intergenic 35.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.2:6781623-6781642 MS.gene95242:CDS 40.0%
AGTGTTTCAGTTTGTCCACT+AGG + chr3.2:6781190-6781209 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.2:6781617-6781636 MS.gene95242:CDS 40.0%
CAGAAACGATGTTTAGGGAA+GGG + chr3.2:6781010-6781029 None:intergenic 40.0%
CATATGTGTGAACAAACTCG+TGG + chr3.2:6781679-6781698 None:intergenic 40.0%
GAATTGCTGGAGAATGATCT+AGG - chr3.2:6781980-6781999 MS.gene95242:CDS 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.2:6781614-6781633 None:intergenic 40.0%
GTCAAAGAACGATTGGACAA+TGG + chr3.2:6781393-6781412 None:intergenic 40.0%
TCACCGAGGTTTAATACGTA+TGG - chr3.2:6782145-6782164 MS.gene95242:CDS 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.2:6781587-6781606 None:intergenic 40.0%
TGTGATGGTCAAAGAACGAT+TGG + chr3.2:6781400-6781419 None:intergenic 40.0%
TTATAAAGTGGTGCGTAGGT+CGG - chr3.2:6782045-6782064 MS.gene95242:CDS 40.0%
! AAAGCTTTGGCAGTTAGAAG+TGG - chr3.2:6782187-6782206 MS.gene95242:CDS 40.0%
! CACGAGTTTGTTCACACATA+TGG - chr3.2:6781677-6781696 MS.gene95242:CDS 40.0%
! CGTGGTGATGTTTAAATGGA+AGG + chr3.2:6781661-6781680 None:intergenic 40.0%
! CTTCTGGAAACCATCTATTG+TGG + chr3.2:6781619-6781638 None:intergenic 40.0%
! GATAGAAATGGAAAAGCGTG+AGG + chr3.2:6781220-6781239 None:intergenic 40.0%
! TTGGTTTGAGTCTTCGATTG+GGG + chr3.2:6781054-6781073 None:intergenic 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.2:6781600-6781619 None:intergenic 40.0%
!! TTTTGAAGAGCAGTCCCTTT+TGG + chr3.2:6781127-6781146 None:intergenic 40.0%
AAGAACGATTGGACAATGGG+TGG + chr3.2:6781389-6781408 None:intergenic 45.0%
AAGACATGGTGTGTCCATCA+TGG + chr3.2:6781433-6781452 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.2:6781907-6781926 MS.gene95242:CDS 45.0%
ACAATGGGTGGGACATCAAT+GGG + chr3.2:6781377-6781396 None:intergenic 45.0%
AGAACGATTGGACAATGGGT+GGG + chr3.2:6781388-6781407 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.2:6781243-6781262 None:intergenic 45.0%
GAGGTGGTTCCATTCTTGTT+CGG + chr3.2:6781901-6781920 None:intergenic 45.0%
GCAGAAACGATGTTTAGGGA+AGG + chr3.2:6781011-6781030 None:intergenic 45.0%
GGCAGCATGGAAAATTCATG+TGG - chr3.2:6782006-6782025 MS.gene95242:CDS 45.0%
GTCGATTGCAAAAACAGTCC+CGG - chr3.2:6781299-6781318 MS.gene95242:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.2:6781244-6781263 None:intergenic 45.0%
TCATATCCAGGTCAAGAAGG+AGG - chr3.2:6782241-6782260 MS.gene95242:CDS 45.0%
TGATGATAAGTGGTTCACCG+AGG - chr3.2:6782131-6782150 MS.gene95242:CDS 45.0%
TGCTGGAGAATGATCTAGGA+TGG - chr3.2:6781984-6782003 MS.gene95242:CDS 45.0%
TGGTTTGAGTCTTCGATTGG+GGG + chr3.2:6781053-6781072 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.2:6781227-6781246 MS.gene95242:CDS 45.0%
! AATGGAAAAGCGTGAGGGAA+AGG + chr3.2:6781214-6781233 None:intergenic 45.0%
! ACCTGAGAAAATGATGGTGC+TGG - chr3.2:6782294-6782313 MS.gene95242:CDS 45.0%
! ATGGAAAAGCGTGAGGGAAA+GGG + chr3.2:6781213-6781232 None:intergenic 45.0%
! GCTGGAGAATGATCTAGGAT+GGG - chr3.2:6781985-6782004 MS.gene95242:CDS 45.0%
! TCCAGCACCATCATTTTCTC+AGG + chr3.2:6782298-6782317 None:intergenic 45.0%
AACGACGGTAGAGGAATTGC+TGG - chr3.2:6781967-6781986 MS.gene95242:CDS 50.0%
AATGGGTGGGACATCAATGG+GGG + chr3.2:6781375-6781394 None:intergenic 50.0%
ATCCAGGTCAAGAAGGAGGA+GGG - chr3.2:6782245-6782264 MS.gene95242:CDS 50.0%
CAATGGGTGGGACATCAATG+GGG + chr3.2:6781376-6781395 None:intergenic 50.0%
CACAAAGCAGTCAGCCATGA+TGG - chr3.2:6781416-6781435 MS.gene95242:CDS 50.0%
CATGGCTGACTGCTTTGTGA+TGG + chr3.2:6781415-6781434 None:intergenic 50.0%
GACAATGGGTGGGACATCAA+TGG + chr3.2:6781378-6781397 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.2:6781232-6781251 None:intergenic 50.0%
GAGTCTTCGATTGGGGGATA+TGG + chr3.2:6781047-6781066 None:intergenic 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.2:6781242-6781261 None:intergenic 50.0%
TATCCAGGTCAAGAAGGAGG+AGG - chr3.2:6782244-6782263 MS.gene95242:CDS 50.0%
TGCGTAGGTCGGTTGAAGAA+TGG - chr3.2:6782056-6782075 MS.gene95242:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.2:6781993-6782012 MS.gene95242:CDS 50.0%
! CATGCACGTCGACTACAACA+TGG - chr3.2:6781842-6781861 MS.gene95242:CDS 50.0%
!! TGCAGTGAGTGCTGAAACGA+CGG - chr3.2:6781952-6781971 MS.gene95242:CDS 50.0%
!! GAGTGCTGAAACGACGGTAG+AGG - chr3.2:6781958-6781977 MS.gene95242:CDS 55.0%
CTCCCTCCTCCTTCTTGACC+TGG + chr3.2:6782250-6782269 None:intergenic 60.0%
GTGGGACATCAATGGGGGTG+AGG + chr3.2:6781370-6781389 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 6780999 6782369 6780999 ID=MS.gene95242
chr3.2 mRNA 6780999 6782369 6780999 ID=MS.gene95242.t1;Parent=MS.gene95242
chr3.2 exon 6780999 6782369 6780999 ID=MS.gene95242.t1.exon1;Parent=MS.gene95242.t1
chr3.2 CDS 6780999 6782369 6780999 ID=cds.MS.gene95242.t1;Parent=MS.gene95242.t1
Gene Sequence

>MS.gene95242

ATGAGCATCCCTTCCCTAAACATCGTTTCTGCATGTTTCATTAAACCATATCCCCCAATCGAAGACTCAAACCAAATATGTCATTTAACACATTGGGATATTGCCATGTTATCTACACATTATAACCAAAAGGGACTGCTCTTCAAAAAACCAACATCATTACTAAACCAACAACATTTCATAGAGAACCTAGTGGACAAACTGAAACACTCCCTTTCCCTCACGCTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTATACTGTTTTCGTCGATTGCAAAAACAGTCCCGGAGCTAAATTTATTCATGCAACTTTAGATATTACTATCAATGATATCCTCACCCCCATTGATGTCCCACCCATTGTCCAATCGTTCTTTGACCATCACAAAGCAGTCAGCCATGATGGACACACCATGTCTTTGTTATCTATTCAAGTCACAGAATTAGTAGATGGTGTTTTCATTGGTTGTTCCATGAATCATTGTCTTTGCGATGGAACATCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATATTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATTATCAACCTTCCATTTAAACATCACCACGAGTTTGTTCACACATATGGAACACCCATTTTAAAAGAGAGAATCTTCCATTTTTCAGCGGAATCTATAGCGAAACTTAAAGCAAAGGCAAATGCAGAGTCTAATACAAACAAAATTTCTTCGTTTCAATCTTTATCAGCATTAGTTTGGAGATCTATAACTCATGCACGTCGACTACAACATGGTCAGAGAACAACTTGCAAAATGGCTACAAACAACCGAACAAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCAATTTATGCAGTGAGTGCTGAAACGACGGTAGAGGAATTGCTGGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATGTGGCTGTTGTAAACCATAATTATAAAGTGGTGCGTAGGTCGGTTGAAGAATGGTTTCGTTCTCCATTTGTGTATCGAACAGATTTGTTTTTTGATCCTTATACTGTGATGATAAGTGGTTCACCGAGGTTTAATACGTATGGAAATGAATTTGGAATGGGAAAAGCTTTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAATTTCATATCCAGGTCAAGAAGGAGGAGGGAGCATAGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGGTGCTGGAGACAGATGAAGAGTTCATGAACTCAGTTTCTGTGTTTAATCCTTTGTTCTAA
Protein sequence

>MS.gene95242.t1

MSIPSLNIVSACFIKPYPPIEDSNQICHLTHWDIAMLSTHYNQKGLLFKKPTSLLNQQHFIENLVDKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILTPIDVPPIVQSFFDHHKAVSHDGHTMSLLSIQVTELVDGVFIGCSMNHCLCDGTSYWNFFNTFSEIFQNDIPVYGPISHQPIHNRWFPEGYGPIINLPFKHHHEFVHTYGTPILKERIFHFSAESIAKLKAKANAESNTNKISSFQSLSALVWRSITHARRLQHGQRTTCKMATNNRTRMEPPLPKEYFGNSIYAVSAETTVEELLENDLGWAAWKIHVAVVNHNYKVVRRSVEEWFRSPFVYRTDLFFDPYTVMISGSPRFNTYGNEFGMGKALAVRSGYANKFDGKVISYPGQEGGGSIDLEVCLSPEKMMVLETDEEFMNSVSVFNPLF