AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012959.t1 MTR_3g101960 91.924 421 34 0 1 421 36 456 0.0 818
MS.gene012959.t1 MTR_3g102020 86.618 411 54 1 1 410 36 446 0.0 749
MS.gene012959.t1 MTR_3g101970 86.375 411 41 2 1 410 36 432 0.0 731
MS.gene012959.t1 MTR_3g102010 80.918 414 71 2 1 410 37 446 0.0 696
MS.gene012959.t1 MTR_3g101900 69.324 414 116 4 1 410 37 443 0.0 583
MS.gene012959.t1 MTR_3g101930 67.064 419 124 5 1 410 37 450 0.0 577
MS.gene012959.t1 MTR_3g102030 74.528 318 55 2 51 368 29 320 3.77e-173 487
MS.gene012959.t1 MTR_4g015180 40.733 464 206 10 1 410 34 482 3.51e-99 305
MS.gene012959.t1 MTR_3g070170 40.088 454 200 13 1 410 40 465 1.75e-98 302
MS.gene012959.t1 MTR_5g081960 39.785 465 208 12 1 410 34 481 2.00e-95 295
MS.gene012959.t1 MTR_4g094842 39.953 423 226 11 2 410 35 443 7.57e-88 274
MS.gene012959.t1 MTR_8g041770 38.916 406 232 10 8 410 44 436 2.11e-83 262
MS.gene012959.t1 MTR_4g022720 39.951 408 223 10 9 410 39 430 2.57e-83 262
MS.gene012959.t1 MTR_4g094835 37.890 417 236 9 2 410 40 441 6.31e-83 261
MS.gene012959.t1 MTR_4g094838 37.705 427 227 9 2 419 36 432 1.42e-82 260
MS.gene012959.t1 MTR_4g022800 38.702 416 226 11 8 410 38 437 5.14e-82 259
MS.gene012959.t1 MTR_0190s0020 37.592 407 238 9 8 410 43 437 3.87e-80 254
MS.gene012959.t1 MTR_4g022825 37.198 414 233 10 8 410 38 435 1.29e-78 250
MS.gene012959.t1 MTR_4g022670 38.313 415 228 11 8 410 38 436 4.37e-78 249
MS.gene012959.t1 MTR_3g088420 37.653 409 231 10 8 410 38 428 2.01e-77 247
MS.gene012959.t1 MTR_7g026860 37.349 415 229 12 8 410 38 433 2.07e-77 247
MS.gene012959.t1 MTR_4g094828 37.838 407 219 13 7 396 42 431 9.34e-76 243
MS.gene012959.t1 MTR_4g022290 35.661 401 226 10 7 394 43 424 2.10e-72 234
MS.gene012959.t1 MTR_3g026310 36.957 414 223 12 9 402 39 434 3.35e-71 231
MS.gene012959.t1 MTR_4g094832 39.554 359 201 7 66 419 83 430 4.09e-71 230
MS.gene012959.t1 MTR_8g079710 36.585 410 227 16 1 396 36 426 3.56e-67 220
MS.gene012959.t1 MTR_4g022760 37.126 334 185 9 8 329 38 358 1.08e-59 199
MS.gene012959.t1 MTR_6g045553 32.287 446 247 11 2 410 43 470 1.03e-58 199
MS.gene012959.t1 MTR_6g016815 29.820 389 197 10 25 410 61 376 1.28e-39 146
MS.gene012959.t1 MTR_8g040780 35.683 227 137 4 199 417 14 239 9.99e-39 140
MS.gene012959.t1 MTR_8g075610 27.296 392 242 15 33 405 47 414 4.01e-34 132
MS.gene012959.t1 MTR_7g026850 42.953 149 74 4 8 155 21 159 4.64e-30 115
MS.gene012959.t1 MTR_2g064495 25.791 411 269 15 13 405 34 426 8.63e-30 120
MS.gene012959.t1 MTR_4g007540 26.859 417 258 16 13 405 34 427 9.64e-30 120
MS.gene012959.t1 MTR_2g105330 25.547 411 270 15 13 405 34 426 2.40e-29 119
MS.gene012959.t1 MTR_7g026770 37.288 177 90 3 120 296 132 287 7.06e-29 115
MS.gene012959.t1 MTR_8g096530 28.571 315 194 10 27 327 53 350 8.26e-28 114
MS.gene012959.t1 MTR_3g098980 28.000 300 195 7 33 323 66 353 2.89e-27 113
MS.gene012959.t1 MTR_3g049150 26.316 418 252 15 21 405 46 440 1.45e-26 111
MS.gene012959.t1 MTR_4g094825 35.450 189 101 5 237 419 218 391 1.05e-25 108
MS.gene012959.t1 MTR_4g094825 30.455 220 122 8 7 209 105 310 2.17e-20 92.8
MS.gene012959.t1 MTR_4g127650 26.601 406 225 16 33 396 66 440 2.98e-25 107
MS.gene012959.t1 MTR_6g034495 38.411 151 85 5 14 158 13 161 9.67e-24 97.4
MS.gene012959.t1 MTR_3g026950 25.234 428 247 18 27 410 48 446 9.97e-24 103
MS.gene012959.t1 MTR_4g097090 26.500 400 234 15 33 396 65 440 1.52e-23 102
MS.gene012959.t1 MTR_6g053810 30.345 290 176 12 34 309 72 349 5.65e-23 100
MS.gene012959.t1 MTR_1g040175 28.261 368 225 17 30 384 61 402 7.48e-22 97.8
MS.gene012959.t1 MTR_8g040760 34.595 185 104 6 8 188 41 212 1.22e-21 93.2
MS.gene012959.t1 MTR_5g076700 24.757 412 275 14 8 401 37 431 1.63e-21 96.7
MS.gene012959.t1 MTR_7g027175 25.562 356 234 10 21 364 56 392 3.32e-21 95.5
MS.gene012959.t1 MTR_7g114470 26.396 394 245 17 30 401 63 433 8.49e-21 94.7
MS.gene012959.t1 MTR_1g040195 24.691 405 266 14 15 401 21 404 1.30e-19 90.9
MS.gene012959.t1 MTR_3g112180 27.089 347 227 11 30 364 58 390 1.57e-19 90.9
MS.gene012959.t1 MTR_1g040215 28.866 291 187 11 30 312 61 339 1.60e-19 90.9
MS.gene012959.t1 MTR_7g114550 25.359 418 264 18 8 401 37 430 1.12e-18 88.2
MS.gene012959.t1 MTR_1g081660 26.598 391 246 17 30 402 70 437 1.76e-18 87.4
MS.gene012959.t1 MTR_7g114960 26.263 396 265 12 30 410 60 443 3.59e-18 86.7
MS.gene012959.t1 MTR_7g086000 26.551 403 234 18 36 398 63 443 6.09e-18 85.9
MS.gene012959.t1 MTR_3g465980 25.407 307 189 11 12 299 49 334 4.79e-17 83.2
MS.gene012959.t1 MTR_3g071320 25.263 380 220 14 11 360 42 387 1.34e-16 81.6
MS.gene012959.t1 MTR_6g034485 35.897 117 68 2 170 282 2 115 1.94e-16 75.9
MS.gene012959.t1 MTR_7g084940 24.038 312 206 10 29 325 65 360 6.11e-16 79.7
MS.gene012959.t1 MTR_7g026830 47.500 80 40 2 66 144 19 97 1.06e-15 72.8
MS.gene012959.t1 MTR_4g055170 24.555 281 185 8 33 299 69 336 1.25e-15 79.0
MS.gene012959.t1 MTR_3g465930 27.240 279 175 9 33 296 69 334 1.27e-15 79.0
MS.gene012959.t1 MTR_1g040315 27.090 299 195 10 1 290 29 313 5.50e-15 76.6
MS.gene012959.t1 MTR_3g465990 25.325 308 188 12 12 299 49 334 5.93e-15 77.0
MS.gene012959.t1 MTR_2g015430 25.418 299 199 10 36 323 61 346 9.82e-14 73.2
MS.gene012959.t1 MTR_5g090830 23.913 322 195 14 13 307 49 347 1.34e-13 72.8
MS.gene012959.t1 MTR_7g014620 23.557 433 274 18 2 406 23 426 2.59e-13 71.6
MS.gene012959.t1 MTR_5g090810 23.602 322 196 14 13 307 49 347 3.23e-13 71.6
MS.gene012959.t1 MTR_7g014230 25.521 384 211 16 25 362 56 410 1.23e-12 69.7
MS.gene012959.t1 MTR_5g090880 23.256 387 243 17 13 365 49 415 1.26e-12 69.7
MS.gene012959.t1 MTR_2g045540 35.227 88 49 1 207 294 27 106 2.72e-12 63.5
MS.gene012959.t1 MTR_7g085990 25.740 338 204 14 15 328 41 355 3.03e-12 68.6
MS.gene012959.t1 MTR_3g049140 24.242 198 136 4 207 390 27 224 4.22e-12 66.2
MS.gene012959.t1 MTR_2g089755 22.886 402 253 14 6 367 49 433 7.50e-12 67.4
MS.gene012959.t1 MTR_7g084910 23.396 265 178 7 29 280 66 318 8.32e-12 67.0
MS.gene012959.t1 MTR_6g015315 24.524 420 267 16 12 401 42 441 1.04e-11 66.6
MS.gene012959.t1 MTR_7g085930 25.291 344 206 14 15 332 45 363 1.10e-11 66.6
MS.gene012959.t1 MTR_0502s0010 29.583 240 145 12 76 302 2 230 2.94e-11 63.5
MS.gene012959.t1 MTR_6g015320 23.645 406 261 15 25 401 58 443 3.90e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012959.t1 AT5G42830 57.314 417 162 4 1 410 37 444 1.65e-175 498
MS.gene012959.t1 AT5G07850 55.961 411 180 1 1 410 38 448 4.68e-172 489
MS.gene012959.t1 AT5G07860 54.831 414 176 5 1 410 38 444 1.66e-158 455
MS.gene012959.t1 AT5G07870 51.537 423 188 3 1 410 38 456 2.61e-155 447
MS.gene012959.t1 AT2G39980 41.394 459 201 9 1 410 36 475 1.78e-104 318
MS.gene012959.t1 AT5G01210 40.625 448 211 10 1 410 34 464 2.12e-93 289
MS.gene012959.t1 AT5G67150 41.007 417 226 11 2 410 37 441 4.22e-93 288
MS.gene012959.t1 AT3G50270 36.429 420 245 12 1 410 36 443 5.46e-76 243
MS.gene012959.t1 AT3G50280 36.493 422 247 12 1 414 34 442 5.11e-73 236
MS.gene012959.t1 AT3G50300 36.139 404 239 9 1 397 34 425 1.07e-72 235
MS.gene012959.t1 AT5G67160 34.878 410 241 11 6 411 41 428 1.44e-66 219
MS.gene012959.t1 AT5G38130 35.396 404 240 11 1 397 54 443 2.67e-66 219
MS.gene012959.t1 AT5G23940 33.333 426 256 11 2 410 33 447 2.84e-60 203
MS.gene012959.t1 AT3G50280 37.662 308 181 7 109 410 6 308 4.93e-54 182
MS.gene012959.t1 AT3G50290 39.300 257 147 6 112 361 15 269 6.81e-45 157
MS.gene012959.t1 AT5G48930 27.470 415 251 16 13 405 41 427 2.86e-31 124
MS.gene012959.t1 AT2G19070 27.381 420 244 17 12 396 42 435 4.95e-28 115
MS.gene012959.t1 AT5G41040 28.205 312 194 9 27 323 63 359 1.35e-26 111
MS.gene012959.t1 AT5G41040 28.013 307 201 7 27 323 79 375 1.77e-26 111
MS.gene012959.t1 AT3G48720 26.289 388 246 14 7 385 40 396 5.37e-22 97.8
MS.gene012959.t1 AT2G40230 27.200 375 214 14 28 374 54 397 6.57e-21 94.7
MS.gene012959.t1 AT5G63560 27.190 331 202 12 7 323 40 345 2.77e-20 92.8
MS.gene012959.t1 AT1G31490 24.634 410 260 16 23 411 52 433 1.05e-18 88.2
MS.gene012959.t1 AT5G57840 25.610 410 265 15 11 401 39 427 1.24e-18 88.2
MS.gene012959.t1 AT1G78990 23.548 310 194 12 13 307 52 333 1.03e-17 85.5
MS.gene012959.t1 AT1G28680 26.174 298 197 10 36 323 65 349 1.12e-17 84.7
MS.gene012959.t1 AT1G28680 26.174 298 197 10 36 323 65 349 1.39e-17 84.7
MS.gene012959.t1 AT1G32910 23.397 312 195 13 13 307 52 336 9.00e-17 82.4
MS.gene012959.t1 AT1G27620 25.000 348 224 11 33 364 58 384 6.26e-15 76.6
MS.gene012959.t1 AT5G16410 22.973 296 181 10 31 307 89 356 3.09e-13 71.6
MS.gene012959.t1 AT1G65450 27.419 186 113 6 131 300 1 180 3.39e-12 67.0
MS.gene012959.t1 AT2G25150 24.667 300 194 11 26 309 60 343 8.83e-12 67.0

Find 103 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.4:-89767593 MS.gene012959:CDS
CAAAATGATGTTCCTGTTTA+TGG 0.097445 3.4:-89767938 MS.gene012959:CDS
GAAGATTCTCTCTCTTAAAA+TGG 0.174060 3.4:+89767805 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.204491 3.4:-89767353 MS.gene012959:CDS
TTGAATGGAAGGTTGATAAT+TGG 0.293176 3.4:+89767863 None:intergenic
TCACCAAGGTTTAATATGTA+TGG 0.306646 3.4:-89767365 MS.gene012959:CDS
AATCTTTATCAGCATTAGTT+TGG 0.307683 3.4:-89767703 MS.gene012959:CDS
CTATAGACTCAGCTGGAAAA+TGG 0.328484 3.4:+89767783 None:intergenic
TCTTGAAGAGAAGTCCCTTT+TGG 0.328911 3.4:+89768386 None:intergenic
CTTCCTGCTCCTTCTTTACC+TGG 0.359731 3.4:+89767263 None:intergenic
AGATGGTTTCCACAAGGTTA+TGG 0.364094 3.4:-89767887 MS.gene012959:CDS
AATGGAAAAGTGTGAGAGAA+AGG 0.389200 3.4:+89768299 None:intergenic
ACAATGGGTGGGACATCAAT+GGG 0.389297 3.4:+89768136 None:intergenic
ATCACCAATACAATGATTCA+TGG 0.399519 3.4:+89768006 None:intergenic
GCCAGAGAAAATGATGTTGC+TGG 0.399927 3.4:-89767216 MS.gene012959:CDS
TTGGTGATGGAACAGCTTAT+TGG 0.406142 3.4:-89767991 MS.gene012959:CDS
TTTCCATTTCTATCCCCTCT+CGG 0.413697 3.4:-89768284 MS.gene012959:CDS
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.4:+89767913 None:intergenic
CAGATGAAGAGTTCAACTTC+TGG 0.424021 3.4:-89767190 MS.gene012959:CDS
ATTTGGAATGGGAAAAGCTT+TGG 0.427618 3.4:-89767336 MS.gene012959:CDS
GGAGAGAACAACTTGCAAAA+TGG 0.431719 3.4:-89767645 MS.gene012959:CDS
ATCAACCTATCCACAATAGA+TGG 0.431811 3.4:-89767904 MS.gene012959:CDS
TATTCAAATCACGGAATTAG+TGG 0.438775 3.4:-89768050 MS.gene012959:CDS
TTCCATTTCTATCCCCTCTC+GGG 0.439902 3.4:-89768283 MS.gene012959:CDS
TGTGAAATGGGACCATAAAC+AGG 0.450206 3.4:+89767926 None:intergenic
TGATCCTTGCACTGTTATGA+TGG 0.455482 3.4:-89767393 MS.gene012959:CDS
ATCTACACACTATATCCAAA+AGG 0.455900 3.4:-89768401 MS.gene012959:CDS
TACAAATAACCGTACGAGAA+TGG 0.458161 3.4:-89767621 MS.gene012959:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3.4:+89768240 None:intergenic
GACAATGGGTGGGACATCAA+TGG 0.465845 3.4:+89768135 None:intergenic
TCAAAGAACGATTGGACAAT+GGG 0.466912 3.4:+89768121 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.4:+89767593 None:intergenic
AAGATTCTCTCTCTTAAAAT+GGG 0.467841 3.4:+89767806 None:intergenic
GAATTCCCAAAATATTCCTT+AGG 0.480035 3.4:+89767587 None:intergenic
GTGCACGTCAACTACAACAC+CGG 0.480060 3.4:-89767667 MS.gene012959:CDS
TGTTCCATGAATCATTGTAT+TGG 0.480232 3.4:-89768010 MS.gene012959:CDS
AACTCATCGTGATGTTTGAA+TGG 0.482751 3.4:+89767848 None:intergenic
GACCCGAGAGGGGATAGAAA+TGG 0.492196 3.4:+89768281 None:intergenic
AGTTTCGCTATAGACTCAGC+TGG 0.493398 3.4:+89767776 None:intergenic
GAATTGCTGGAGAATGATCT+AGG 0.495485 3.4:-89767530 MS.gene012959:CDS
CACCAAGGTTTAATATGTAT+GGG 0.497351 3.4:-89767364 MS.gene012959:CDS
TTTGTTATCTATTCAAATCA+CGG 0.498414 3.4:-89768059 MS.gene012959:CDS
ATGGAAAAGTGTGAGAGAAA+GGG 0.500507 3.4:+89768300 None:intergenic
TTCCCATACATATTAAACCT+TGG 0.501336 3.4:+89767362 None:intergenic
TGTTGGTTTAGTGATGATGT+TGG 0.506905 3.4:+89768361 None:intergenic
GACAAAGCAGTCAGCCATGA+TGG 0.509313 3.4:-89768094 MS.gene012959:CDS
CAATGGGTGGGACATCAATG+GGG 0.513002 3.4:+89768137 None:intergenic
AGTGTTTGAGTTTGTCCACC+AGG 0.515596 3.4:+89768323 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3.4:-89767347 MS.gene012959:CDS
ACATCATATCCAGGTAAAGA+AGG 0.522329 3.4:-89767272 MS.gene012959:CDS
TGCTGGAGAATGATCTAGGA+TGG 0.523136 3.4:-89767526 MS.gene012959:CDS
GGTTCTCAATGAAGTGCTGT+TGG 0.527425 3.4:+89768344 None:intergenic
TGTCATGATCAAAGAACGAT+TGG 0.527909 3.4:+89768113 None:intergenic
AAATCTGTTTGATAAACAAA+AGG 0.527952 3.4:+89767422 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3.4:-89768004 MS.gene012959:CDS
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.4:+89767914 None:intergenic
CATCGTGATGTTTGAATGGA+AGG 0.541756 3.4:+89767852 None:intergenic
GGAAAAGTAACATCATATCC+AGG 0.542909 3.4:-89767281 MS.gene012959:CDS
GGATATGCTAACAAATTCGA+TGG 0.547641 3.4:-89767302 MS.gene012959:CDS
GATCCTTGCACTGTTATGAT+GGG 0.548259 3.4:-89767392 MS.gene012959:CDS
TGCGGTGAGTGCTGAAACGA+CGG 0.549616 3.4:-89767558 MS.gene012959:CDS
TATCCAGGTAAAGAAGGAGC+AGG 0.550153 3.4:-89767266 MS.gene012959:CDS
AATAAATCATGATGATAAAG+TGG 0.561465 3.4:-89767477 MS.gene012959:CDS
AAAGCTTTGGCAGTTAGAAG+TGG 0.567341 3.4:-89767323 MS.gene012959:CDS
TCTACACACTATATCCAAAA+GGG 0.567974 3.4:-89768400 MS.gene012959:CDS
AATGGGTGGGACATCAATGG+GGG 0.568371 3.4:+89768138 None:intergenic
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.4:-89767517 MS.gene012959:CDS
ATCAAAGAACGATTGGACAA+TGG 0.570569 3.4:+89768120 None:intergenic
AATGGAACCACCTCTTCCTA+AGG 0.571584 3.4:-89767603 MS.gene012959:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3.4:-89767348 MS.gene012959:CDS
ACAGCACTTCATTGAGAACC+TGG 0.575720 3.4:-89768341 MS.gene012959:CDS
GAGTGCTGAAACGACGGTGG+GGG 0.585490 3.4:-89767552 MS.gene012959:CDS
ACGAAAACAGTGTAAGAATG+AGG 0.585728 3.4:+89768232 None:intergenic
CACAATAGATGGTTTCCACA+AGG 0.586552 3.4:-89767893 MS.gene012959:CDS
GAGGTGGTTCCATTCTCGTA+CGG 0.591000 3.4:+89767612 None:intergenic
TGCACGTCAACTACAACACC+GGG 0.593354 3.4:-89767666 MS.gene012959:CDS
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.4:+89767596 None:intergenic
TGCGTAGGTCGGTTGAAGAG+TGG 0.594966 3.4:-89767454 MS.gene012959:CDS
AGTAACAAGACGACCCGAGA+GGG 0.598981 3.4:+89768270 None:intergenic
GTGAGTGCTGAAACGACGGT+GGG 0.603452 3.4:-89767554 MS.gene012959:CDS
ATACTCAAAGACTAGAACAT+GGG 0.612835 3.4:+89767159 None:intergenic
TGAGTGCTGAAACGACGGTG+GGG 0.614267 3.4:-89767553 MS.gene012959:CDS
CTACCCATCATAACAGTGCA+AGG 0.614444 3.4:+89767389 None:intergenic
AAGAACGATTGGACAATGGG+TGG 0.614907 3.4:+89768124 None:intergenic
CATACTCAAAGACTAGAACA+TGG 0.617067 3.4:+89767158 None:intergenic
AGAACGATTGGACAATGGGT+GGG 0.617288 3.4:+89768125 None:intergenic
AAGACATGGTATGACCATCA+TGG 0.620179 3.4:+89768080 None:intergenic
GGAAACCATCTATTGTGGAT+AGG 0.621301 3.4:+89767899 None:intergenic
GATATAGTGTGTAGATAACA+TGG 0.622938 3.4:+89768408 None:intergenic
GTCGATTGCAACAACAGTCG+CGG 0.624393 3.4:-89768211 MS.gene012959:CDS
GCTGGAGAATGATCTAGGAT+GGG 0.628974 3.4:-89767525 MS.gene012959:CDS
ATGATGATAAAGTGGTGCGT+AGG 0.641507 3.4:-89767469 MS.gene012959:CDS
ATAATTGGACCATAACCTTG+TGG 0.645522 3.4:+89767878 None:intergenic
TATAGCGAAACTGAAAGCAA+AGG 0.650352 3.4:-89767765 MS.gene012959:CDS
CTTGTGGAAACCATCTATTG+TGG 0.654333 3.4:+89767894 None:intergenic
TGATAAAGTGGTGCGTAGGT+CGG 0.663573 3.4:-89767465 MS.gene012959:CDS
CAAATCACGGAATTAGTGGA+CGG 0.665273 3.4:-89768046 MS.gene012959:CDS
GGTGAGTGCTGAAACGACGG+TGG 0.672156 3.4:-89767555 MS.gene012959:CDS
GTAACAAGACGACCCGAGAG+GGG 0.677630 3.4:+89768271 None:intergenic
TTGAATAGATAACAAAGACA+TGG 0.692883 3.4:+89768066 None:intergenic
TTATGATGGGTAGTTCACCA+AGG 0.706961 3.4:-89767379 MS.gene012959:CDS
GCACTTCATTGAGAACCTGG+TGG 0.715971 3.4:-89768338 MS.gene012959:CDS
CAGTAACAAGACGACCCGAG+AGG 0.727558 3.4:+89768269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTGTTTGATAAACAAA+AGG + chr3.4:89768150-89768169 None:intergenic 20.0%
!! AATAAATCATGATGATAAAG+TGG - chr3.4:89768092-89768111 MS.gene012959:CDS 20.0%
!!! TTTGTTATCTATTCAAATCA+CGG - chr3.4:89767510-89767529 MS.gene012959:CDS 20.0%
! AAGATTCTCTCTCTTAAAAT+GGG + chr3.4:89767766-89767785 None:intergenic 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.4:89768216-89768235 MS.gene012959:CDS 25.0%
! AATCTTTATCAGCATTAGTT+TGG - chr3.4:89767866-89767885 MS.gene012959:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.4:89767506-89767525 None:intergenic 25.0%
ATCACCAATACAATGATTCA+TGG + chr3.4:89767566-89767585 None:intergenic 30.0%
ATCTACACACTATATCCAAA+AGG - chr3.4:89767168-89767187 MS.gene012959:CDS 30.0%
CAAAATGATGTTCCTGTTTA+TGG - chr3.4:89767631-89767650 MS.gene012959:CDS 30.0%
CACCAAGGTTTAATATGTAT+GGG - chr3.4:89768205-89768224 MS.gene012959:CDS 30.0%
GAAGATTCTCTCTCTTAAAA+TGG + chr3.4:89767767-89767786 None:intergenic 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.4:89767985-89768004 None:intergenic 30.0%
TATTCAAATCACGGAATTAG+TGG - chr3.4:89767519-89767538 MS.gene012959:CDS 30.0%
TCACCAAGGTTTAATATGTA+TGG - chr3.4:89768204-89768223 MS.gene012959:CDS 30.0%
TCTACACACTATATCCAAAA+GGG - chr3.4:89767169-89767188 MS.gene012959:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - chr3.4:89767559-89767578 MS.gene012959:CDS 30.0%
TTCCCATACATATTAAACCT+TGG + chr3.4:89768210-89768229 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - chr3.4:89767565-89767584 MS.gene012959:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.4:89767977-89767996 MS.gene012959:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.4:89768222-89768241 MS.gene012959:CDS 30.0%
!! TTGAATGGAAGGTTGATAAT+TGG + chr3.4:89767709-89767728 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.4:89767976-89767995 None:intergenic 35.0%
AACTCATCGTGATGTTTGAA+TGG + chr3.4:89767724-89767743 None:intergenic 35.0%
ACATCATATCCAGGTAAAGA+AGG - chr3.4:89768297-89768316 MS.gene012959:CDS 35.0%
ACGAAAACAGTGTAAGAATG+AGG + chr3.4:89767340-89767359 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + chr3.4:89767332-89767351 None:intergenic 35.0%
ATAATTGGACCATAACCTTG+TGG + chr3.4:89767694-89767713 None:intergenic 35.0%
ATCAAAGAACGATTGGACAA+TGG + chr3.4:89767452-89767471 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.4:89767665-89767684 MS.gene012959:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.4:89767979-89767998 None:intergenic 35.0%
GGAAAAGTAACATCATATCC+AGG - chr3.4:89768288-89768307 MS.gene012959:CDS 35.0%
GGATATGCTAACAAATTCGA+TGG - chr3.4:89768267-89768286 MS.gene012959:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.4:89768221-89768240 MS.gene012959:CDS 35.0%
TACAAATAACCGTACGAGAA+TGG - chr3.4:89767948-89767967 MS.gene012959:CDS 35.0%
TATAGCGAAACTGAAAGCAA+AGG - chr3.4:89767804-89767823 MS.gene012959:CDS 35.0%
TCAAAGAACGATTGGACAAT+GGG + chr3.4:89767451-89767470 None:intergenic 35.0%
TGTCATGATCAAAGAACGAT+TGG + chr3.4:89767459-89767478 None:intergenic 35.0%
! AATGGAAAAGTGTGAGAGAA+AGG + chr3.4:89767273-89767292 None:intergenic 35.0%
! ATGGAAAAGTGTGAGAGAAA+GGG + chr3.4:89767272-89767291 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCTT+TGG - chr3.4:89768233-89768252 MS.gene012959:CDS 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.4:89767976-89767995 MS.gene012959:CDS 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.4:89767658-89767677 None:intergenic 35.0%
!! TGTTGGTTTAGTGATGATGT+TGG + chr3.4:89767211-89767230 None:intergenic 35.0%
!! TTTTGGGAATTCACTTCATG+CGG - chr3.4:89767993-89768012 MS.gene012959:CDS 35.0%
AAGACATGGTATGACCATCA+TGG + chr3.4:89767492-89767511 None:intergenic 40.0%
AGATGGTTTCCACAAGGTTA+TGG - chr3.4:89767682-89767701 MS.gene012959:CDS 40.0%
ATGATGATAAAGTGGTGCGT+AGG - chr3.4:89768100-89768119 MS.gene012959:CDS 40.0%
CAAATCACGGAATTAGTGGA+CGG - chr3.4:89767523-89767542 MS.gene012959:CDS 40.0%
CACAATAGATGGTTTCCACA+AGG - chr3.4:89767676-89767695 MS.gene012959:CDS 40.0%
CAGATGAAGAGTTCAACTTC+TGG - chr3.4:89768379-89768398 MS.gene012959:CDS 40.0%
CATCGTGATGTTTGAATGGA+AGG + chr3.4:89767720-89767739 None:intergenic 40.0%
CTATAGACTCAGCTGGAAAA+TGG + chr3.4:89767789-89767808 None:intergenic 40.0%
CTTGTGGAAACCATCTATTG+TGG + chr3.4:89767678-89767697 None:intergenic 40.0%
GAATTGCTGGAGAATGATCT+AGG - chr3.4:89768039-89768058 MS.gene012959:CDS 40.0%
GATCCTTGCACTGTTATGAT+GGG - chr3.4:89768177-89768196 MS.gene012959:CDS 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.4:89767673-89767692 None:intergenic 40.0%
GGAGAGAACAACTTGCAAAA+TGG - chr3.4:89767924-89767943 MS.gene012959:CDS 40.0%
TGATCCTTGCACTGTTATGA+TGG - chr3.4:89768176-89768195 MS.gene012959:CDS 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.4:89767646-89767665 None:intergenic 40.0%
TTTCCATTTCTATCCCCTCT+CGG - chr3.4:89767285-89767304 MS.gene012959:CDS 40.0%
! AAAGCTTTGGCAGTTAGAAG+TGG - chr3.4:89768246-89768265 MS.gene012959:CDS 40.0%
! TCCAGCAACATCATTTTCTC+TGG + chr3.4:89768357-89768376 None:intergenic 40.0%
! TCTTGAAGAGAAGTCCCTTT+TGG + chr3.4:89767186-89767205 None:intergenic 40.0%
! TTTTGCAAGTTGTTCTCTCC+CGG + chr3.4:89767924-89767943 None:intergenic 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.4:89767659-89767678 None:intergenic 40.0%
!! TTAGTGGACGGTGTTTTCAT+TGG - chr3.4:89767535-89767554 MS.gene012959:CDS 40.0%
!! TTATGATGGGTAGTTCACCA+AGG - chr3.4:89768190-89768209 MS.gene012959:CDS 40.0%
!! TTGGTGATGGAACAGCTTAT+TGG - chr3.4:89767578-89767597 MS.gene012959:CDS 40.0%
AAGAACGATTGGACAATGGG+TGG + chr3.4:89767448-89767467 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.4:89767966-89767985 MS.gene012959:CDS 45.0%
ACAATGGGTGGGACATCAAT+GGG + chr3.4:89767436-89767455 None:intergenic 45.0%
ACAGCACTTCATTGAGAACC+TGG - chr3.4:89767228-89767247 MS.gene012959:CDS 45.0%
AGAACGATTGGACAATGGGT+GGG + chr3.4:89767447-89767466 None:intergenic 45.0%
AGTTTCGCTATAGACTCAGC+TGG + chr3.4:89767796-89767815 None:intergenic 45.0%
CTACCCATCATAACAGTGCA+AGG + chr3.4:89768183-89768202 None:intergenic 45.0%
GCCAGAGAAAATGATGTTGC+TGG - chr3.4:89768353-89768372 MS.gene012959:CDS 45.0%
GGGGGTGAGGATATCATTTA+TGG + chr3.4:89767416-89767435 None:intergenic 45.0%
TATCCAGGTAAAGAAGGAGC+AGG - chr3.4:89768303-89768322 MS.gene012959:CDS 45.0%
TGATAAAGTGGTGCGTAGGT+CGG - chr3.4:89768104-89768123 MS.gene012959:CDS 45.0%
TGCTGGAGAATGATCTAGGA+TGG - chr3.4:89768043-89768062 MS.gene012959:CDS 45.0%
TTCCATTTCTATCCCCTCTC+GGG - chr3.4:89767286-89767305 MS.gene012959:CDS 45.0%
! AGTGTTTGAGTTTGTCCACC+AGG + chr3.4:89767249-89767268 None:intergenic 45.0%
! GCTGGAGAATGATCTAGGAT+GGG - chr3.4:89768044-89768063 MS.gene012959:CDS 45.0%
!! GGTTCTCAATGAAGTGCTGT+TGG + chr3.4:89767228-89767247 None:intergenic 45.0%
AATGGGTGGGACATCAATGG+GGG + chr3.4:89767434-89767453 None:intergenic 50.0%
AGTAACAAGACGACCCGAGA+GGG + chr3.4:89767302-89767321 None:intergenic 50.0%
CAATGGGTGGGACATCAATG+GGG + chr3.4:89767435-89767454 None:intergenic 50.0%
CTTCCTGCTCCTTCTTTACC+TGG + chr3.4:89768309-89768328 None:intergenic 50.0%
GACAAAGCAGTCAGCCATGA+TGG - chr3.4:89767475-89767494 MS.gene012959:CDS 50.0%
GACAATGGGTGGGACATCAA+TGG + chr3.4:89767437-89767456 None:intergenic 50.0%
GAGGTGGTTCCATTCTCGTA+CGG + chr3.4:89767960-89767979 None:intergenic 50.0%
GCACTTCATTGAGAACCTGG+TGG - chr3.4:89767231-89767250 MS.gene012959:CDS 50.0%
GTCGATTGCAACAACAGTCG+CGG - chr3.4:89767358-89767377 MS.gene012959:CDS 50.0%
GTGCACGTCAACTACAACAC+CGG - chr3.4:89767902-89767921 MS.gene012959:CDS 50.0%
TGCACGTCAACTACAACACC+GGG - chr3.4:89767903-89767922 MS.gene012959:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.4:89768052-89768071 MS.gene012959:CDS 50.0%
CAGTAACAAGACGACCCGAG+AGG + chr3.4:89767303-89767322 None:intergenic 55.0%
GACCCGAGAGGGGATAGAAA+TGG + chr3.4:89767291-89767310 None:intergenic 55.0%
GTAACAAGACGACCCGAGAG+GGG + chr3.4:89767301-89767320 None:intergenic 55.0%
TGCGTAGGTCGGTTGAAGAG+TGG - chr3.4:89768115-89768134 MS.gene012959:CDS 55.0%
!! GTGAGTGCTGAAACGACGGT+GGG - chr3.4:89768015-89768034 MS.gene012959:CDS 55.0%
!! TGAGTGCTGAAACGACGGTG+GGG - chr3.4:89768016-89768035 MS.gene012959:CDS 55.0%
!! TGCGGTGAGTGCTGAAACGA+CGG - chr3.4:89768011-89768030 MS.gene012959:CDS 55.0%
AACGACGGTGGGGGAATTGC+TGG - chr3.4:89768026-89768045 MS.gene012959:CDS 60.0%
GTGGGACATCAATGGGGGTG+AGG + chr3.4:89767429-89767448 None:intergenic 60.0%
!! GAGTGCTGAAACGACGGTGG+GGG - chr3.4:89768017-89768036 MS.gene012959:CDS 60.0%
!! GGTGAGTGCTGAAACGACGG+TGG - chr3.4:89768014-89768033 MS.gene012959:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89767163 89768428 89767163 ID=MS.gene012959
chr3.4 mRNA 89767163 89768428 89767163 ID=MS.gene012959.t1;Parent=MS.gene012959
chr3.4 exon 89767163 89768428 89767163 ID=MS.gene012959.t1.exon1;Parent=MS.gene012959.t1
chr3.4 CDS 89767163 89768428 89767163 ID=cds.MS.gene012959.t1;Parent=MS.gene012959.t1
Gene Sequence

>MS.gene012959

ATGTTATCTACACACTATATCCAAAAGGGACTTCTCTTCAAGAAACCAACATCATCACTAAACCAACAGCACTTCATTGAGAACCTGGTGGACAAACTCAAACACTCCCTTTCTCTCACACTTTTCCATTTCTATCCCCTCTCGGGTCGTCTTGTTACTGAAAAATCCGAAGATCCTCATTCTTACACTGTTTTCGTCGATTGCAACAACAGTCGCGGAGCTAAATTTATTCATGCAACTTTAGATATCACCATAAATGATATCCTCACCCCCATTGATGTCCCACCCATTGTCCAATCGTTCTTTGATCATGACAAAGCAGTCAGCCATGATGGTCATACCATGTCTTTGTTATCTATTCAAATCACGGAATTAGTGGACGGTGTTTTCATTGGTTGTTCCATGAATCATTGTATTGGTGATGGAACAGCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATGTTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCACAAGGTTATGGTCCAATTATCAACCTTCCATTCAAACATCACGATGAGTTTGTTCACAGATATGAAACACCCATTTTAAGAGAGAGAATCTTCCATTTTCCAGCTGAGTCTATAGCGAAACTGAAAGCAAAGGCAAATGAAGTGTCTAATACAAGCAAAATTTCTTCGTTTCAATCTTTATCAGCATTAGTTTGGAGATCTATAACTTGTGCACGTCAACTACAACACCGGGAGAGAACAACTTGCAAAATGGCTACAAATAACCGTACGAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCACTTCATGCGGTGAGTGCTGAAACGACGGTGGGGGAATTGCTGGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATCTAGCTGTAATAAATCATGATGATAAAGTGGTGCGTAGGTCGGTTGAAGAGTGGTTTCGTTCTCCTTTTGTTTATCAAACAGATTTGTTTTTTGATCCTTGCACTGTTATGATGGGTAGTTCACCAAGGTTTAATATGTATGGGAATGAATTTGGAATGGGAAAAGCTTTGGCAGTTAGAAGTGGATATGCTAACAAATTCGATGGAAAAGTAACATCATATCCAGGTAAAGAAGGAGCAGGAAGCATTGATCTTGAAGTGTGTCTTTCGCCAGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCAACTTCTGGTGTTTGCTCCCATGTTCTAGTCTTTGA
Protein sequence

>MS.gene012959.t1

MLSTHYIQKGLLFKKPTSSLNQQHFIENLVDKLKHSLSLTLFHFYPLSGRLVTEKSEDPHSYTVFVDCNNSRGAKFIHATLDITINDILTPIDVPPIVQSFFDHDKAVSHDGHTMSLLSIQITELVDGVFIGCSMNHCIGDGTAYWNFFNTFSEIFQNDVPVYGPISHQPIHNRWFPQGYGPIINLPFKHHDEFVHRYETPILRERIFHFPAESIAKLKAKANEVSNTSKISSFQSLSALVWRSITCARQLQHRERTTCKMATNNRTRMEPPLPKEYFGNSLHAVSAETTVGELLENDLGWAAWKIHLAVINHDDKVVRRSVEEWFRSPFVYQTDLFFDPCTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGKEGAGSIDLEVCLSPEKMMLLETDEEFNFWCLLPCSSL