AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38703

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38703.t1 MTR_3g101930 93.833 454 28 0 1 454 2 455 0.0 899
MS.gene38703.t1 MTR_3g101900 72.907 454 114 3 1 453 2 447 0.0 691
MS.gene38703.t1 MTR_3g101960 69.058 446 124 5 9 449 9 445 0.0 632
MS.gene38703.t1 MTR_3g102020 67.713 446 139 3 9 453 9 450 0.0 630
MS.gene38703.t1 MTR_3g101970 67.040 446 128 3 9 453 9 436 0.0 624
MS.gene38703.t1 MTR_3g102010 63.956 455 156 5 1 453 2 450 0.0 598
MS.gene38703.t1 MTR_3g102030 56.832 322 109 4 87 408 29 320 2.28e-130 379
MS.gene38703.t1 MTR_4g094842 41.031 446 236 11 16 450 15 444 3.24e-100 307
MS.gene38703.t1 MTR_0190s0020 40.363 441 242 9 18 453 17 441 3.62e-99 304
MS.gene38703.t1 MTR_4g094835 40.783 434 230 9 25 450 28 442 1.63e-96 298
MS.gene38703.t1 MTR_4g022800 39.686 446 236 12 21 453 16 441 8.96e-96 295
MS.gene38703.t1 MTR_3g070170 37.885 454 222 9 33 450 37 466 2.73e-95 295
MS.gene38703.t1 MTR_8g041770 39.030 433 234 9 29 453 30 440 4.34e-94 291
MS.gene38703.t1 MTR_4g022720 40.411 438 235 10 21 452 16 433 1.44e-93 290
MS.gene38703.t1 MTR_4g022670 38.652 445 241 10 21 453 16 440 8.36e-93 288
MS.gene38703.t1 MTR_3g088420 38.991 436 240 10 23 453 18 432 9.13e-92 285
MS.gene38703.t1 MTR_4g094838 37.819 431 231 7 28 450 27 428 2.31e-91 284
MS.gene38703.t1 MTR_7g026860 38.149 443 241 11 21 452 16 436 2.78e-90 281
MS.gene38703.t1 MTR_4g015180 37.475 507 242 12 1 453 1 486 4.14e-88 277
MS.gene38703.t1 MTR_4g022825 37.723 448 248 10 17 453 12 439 7.88e-88 275
MS.gene38703.t1 MTR_5g081960 35.573 506 246 12 1 450 1 482 1.34e-87 276
MS.gene38703.t1 MTR_3g026310 37.668 446 235 11 29 453 24 447 2.14e-83 264
MS.gene38703.t1 MTR_4g094832 41.834 349 190 4 107 450 86 426 9.35e-83 261
MS.gene38703.t1 MTR_4g094828 37.277 448 243 13 23 453 23 449 2.82e-81 258
MS.gene38703.t1 MTR_4g022290 35.714 462 258 11 7 452 5 443 5.72e-80 254
MS.gene38703.t1 MTR_8g079710 37.071 437 238 12 21 443 21 434 4.27e-75 242
MS.gene38703.t1 MTR_4g022760 35.281 445 212 11 21 453 16 396 1.62e-73 236
MS.gene38703.t1 MTR_6g045553 29.876 482 275 12 10 453 18 474 4.11e-52 182
MS.gene38703.t1 MTR_6g016815 31.264 435 209 13 23 453 32 380 1.98e-50 176
MS.gene38703.t1 MTR_8g040780 37.113 194 119 2 245 435 20 213 1.74e-36 135
MS.gene38703.t1 MTR_8g075610 28.378 370 214 14 69 417 47 386 2.16e-34 133
MS.gene38703.t1 MTR_4g007540 28.226 372 227 13 55 409 44 392 3.25e-34 133
MS.gene38703.t1 MTR_8g096530 32.595 316 175 12 63 362 53 346 5.95e-34 132
MS.gene38703.t1 MTR_2g064495 26.847 406 258 12 55 444 44 426 1.16e-33 131
MS.gene38703.t1 MTR_7g026850 44.586 157 72 5 43 198 21 163 1.22e-33 125
MS.gene38703.t1 MTR_2g105330 26.847 406 258 12 55 444 44 426 2.36e-33 130
MS.gene38703.t1 MTR_3g098980 30.492 305 183 9 69 362 66 352 2.45e-32 128
MS.gene38703.t1 MTR_3g026950 25.304 411 242 12 63 433 48 433 1.08e-29 120
MS.gene38703.t1 MTR_7g026770 41.209 182 83 5 157 338 132 289 1.30e-29 117
MS.gene38703.t1 MTR_8g040760 36.937 222 122 6 9 228 7 212 3.58e-29 114
MS.gene38703.t1 MTR_3g049150 29.032 310 184 9 56 349 45 334 8.43e-28 115
MS.gene38703.t1 MTR_4g127650 25.855 468 285 19 9 444 12 449 1.00e-27 115
MS.gene38703.t1 MTR_6g034495 44.262 122 63 3 80 198 45 164 3.72e-27 107
MS.gene38703.t1 MTR_4g094825 37.931 145 80 1 306 450 253 387 2.27e-26 110
MS.gene38703.t1 MTR_4g094825 37.725 167 95 3 29 194 92 250 5.38e-25 106
MS.gene38703.t1 MTR_7g027175 27.094 406 251 14 56 444 55 432 9.35e-25 106
MS.gene38703.t1 MTR_4g097090 25.170 441 259 16 13 417 16 421 2.58e-24 105
MS.gene38703.t1 MTR_3g112180 27.221 349 228 9 66 404 58 390 2.73e-24 105
MS.gene38703.t1 MTR_5g076700 25.172 437 270 18 43 451 37 444 5.38e-23 101
MS.gene38703.t1 MTR_7g084940 28.490 351 210 12 67 402 67 391 8.36e-23 100
MS.gene38703.t1 MTR_1g040195 28.919 370 220 16 67 423 37 376 8.95e-23 100
MS.gene38703.t1 MTR_7g086000 30.225 311 180 13 72 362 63 356 3.43e-22 99.4
MS.gene38703.t1 MTR_6g015815 25.780 481 279 18 6 443 7 452 7.45e-22 98.2
MS.gene38703.t1 MTR_7g114520 28.537 410 237 21 69 451 66 446 4.67e-21 95.9
MS.gene38703.t1 MTR_1g053315 25.661 378 241 15 54 415 63 416 1.25e-20 94.4
MS.gene38703.t1 MTR_6g053810 29.066 289 187 10 69 349 71 349 1.41e-20 94.4
MS.gene38703.t1 MTR_3g071320 26.420 352 217 12 68 400 59 387 2.85e-20 93.2
MS.gene38703.t1 MTR_7g114470 25.169 445 261 21 43 451 38 446 3.48e-20 93.2
MS.gene38703.t1 MTR_1g040175 26.705 352 214 13 69 404 64 387 1.95e-19 90.9
MS.gene38703.t1 MTR_7g114490 26.728 434 250 21 43 444 37 434 2.19e-19 90.9
MS.gene38703.t1 MTR_7g085990 25.581 430 259 20 55 444 42 450 3.45e-19 90.1
MS.gene38703.t1 MTR_7g114550 25.694 432 273 19 43 451 37 443 3.49e-19 90.1
MS.gene38703.t1 MTR_1g040215 26.292 445 267 22 1 424 1 405 6.92e-19 89.4
MS.gene38703.t1 MTR_3g028210 24.760 416 268 14 55 440 53 453 1.07e-18 88.6
MS.gene38703.t1 MTR_4g013050 25.517 290 166 9 67 350 51 296 1.07e-18 88.2
MS.gene38703.t1 MTR_3g465980 27.152 302 185 11 47 334 49 329 1.29e-18 88.2
MS.gene38703.t1 MTR_7g014330 26.034 411 239 16 29 402 26 408 1.32e-18 88.2
MS.gene38703.t1 MTR_6g015850 26.340 429 239 15 56 443 57 449 1.47e-18 88.2
MS.gene38703.t1 MTR_7g114560 25.694 432 273 19 43 451 37 443 1.53e-18 88.2
MS.gene38703.t1 MTR_8g012750 27.083 384 234 17 55 402 48 421 1.67e-18 88.2
MS.gene38703.t1 MTR_7g026830 51.429 70 33 1 113 181 28 97 3.33e-18 80.1
MS.gene38703.t1 MTR_3g465990 28.082 292 178 11 57 334 56 329 3.67e-18 87.0
MS.gene38703.t1 MTR_8g079970 26.415 371 228 17 55 409 46 387 4.01e-18 86.7
MS.gene38703.t1 MTR_4g055170 28.571 280 169 9 69 334 69 331 4.40e-18 86.7
MS.gene38703.t1 MTR_3g450710 24.599 374 229 14 63 404 59 411 4.58e-18 86.7
MS.gene38703.t1 MTR_6g015830 24.350 423 258 12 56 443 57 452 5.18e-18 86.7
MS.gene38703.t1 MTR_7g085930 25.871 402 231 16 55 412 46 424 7.47e-18 86.3
MS.gene38703.t1 MTR_1g081660 26.102 295 188 12 66 349 70 345 1.11e-17 85.5
MS.gene38703.t1 MTR_7g084910 28.358 268 161 8 67 320 68 318 1.75e-17 84.7
MS.gene38703.t1 MTR_6g034485 37.391 115 71 1 209 322 1 115 4.92e-17 77.4
MS.gene38703.t1 MTR_1g040315 27.794 349 197 16 1 330 1 313 1.29e-16 82.0
MS.gene38703.t1 MTR_7g014230 25.728 412 239 15 29 402 28 410 4.60e-16 80.5
MS.gene38703.t1 MTR_3g465910 27.599 279 170 10 70 334 69 329 5.12e-16 80.9
MS.gene38703.t1 MTR_3g465930 25.362 276 184 6 69 334 69 332 8.17e-16 79.7
MS.gene38703.t1 MTR_5g090880 25.401 374 215 16 69 404 67 414 3.19e-15 78.2
MS.gene38703.t1 MTR_7g014250 25.935 428 249 17 55 442 48 447 5.07e-15 77.4
MS.gene38703.t1 MTR_2g089755 24.065 428 245 17 29 407 37 433 5.95e-15 77.4
MS.gene38703.t1 MTR_3g450690 23.324 373 232 14 63 404 66 415 1.82e-14 75.9
MS.gene38703.t1 MTR_3g465880 27.239 268 166 10 69 321 68 321 2.90e-14 75.1
MS.gene38703.t1 MTR_3g465950 24.809 262 178 7 70 322 69 320 3.58e-14 74.7
MS.gene38703.t1 MTR_6g015315 24.880 418 251 16 61 443 55 444 4.19e-14 74.3
MS.gene38703.t1 MTR_0502s0010 30.992 242 142 11 113 342 2 230 4.29e-14 72.0
MS.gene38703.t1 MTR_7g056087 23.795 332 218 12 55 367 45 360 4.36e-14 74.3
MS.gene38703.t1 MTR_5g090830 23.577 369 227 13 69 404 66 412 1.26e-13 73.2
MS.gene38703.t1 MTR_2g089650 21.396 444 287 18 29 435 29 447 2.10e-13 72.4
MS.gene38703.t1 MTR_5g090810 23.306 369 228 13 69 404 66 412 1.54e-12 69.7
MS.gene38703.t1 MTR_1g094790 25.943 424 240 20 62 450 53 437 8.37e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38703.t1 AT5G42830 53.394 442 198 3 13 453 14 448 1.35e-169 484
MS.gene38703.t1 AT5G07850 51.982 454 211 5 2 453 4 452 2.41e-163 469
MS.gene38703.t1 AT5G07860 49.888 447 213 6 9 453 11 448 1.42e-146 426
MS.gene38703.t1 AT5G07870 47.966 467 218 5 2 453 4 460 2.10e-145 423
MS.gene38703.t1 AT5G67150 39.696 461 239 13 9 453 8 445 4.19e-102 312
MS.gene38703.t1 AT2G39980 37.580 471 220 11 33 453 33 479 5.17e-95 295
MS.gene38703.t1 AT3G50270 36.819 459 256 10 9 453 9 447 4.29e-92 286
MS.gene38703.t1 AT5G01210 37.398 492 245 12 1 453 1 468 3.23e-88 277
MS.gene38703.t1 AT3G50280 36.303 449 263 8 9 450 7 439 3.65e-88 276
MS.gene38703.t1 AT5G38130 36.782 435 248 13 9 435 27 442 2.30e-82 261
MS.gene38703.t1 AT3G50300 34.348 460 269 10 9 454 7 447 2.42e-82 261
MS.gene38703.t1 AT5G67160 35.681 426 244 11 28 449 28 427 4.74e-75 242
MS.gene38703.t1 AT3G50280 39.048 315 181 4 141 450 1 309 2.88e-67 218
MS.gene38703.t1 AT5G23940 31.140 456 287 11 9 450 6 448 7.69e-60 203
MS.gene38703.t1 AT3G50290 40.769 260 142 5 149 401 15 269 5.98e-52 176
MS.gene38703.t1 AT2G19070 29.933 451 250 20 29 444 25 444 2.39e-37 142
MS.gene38703.t1 AT5G48930 27.644 416 243 13 55 444 44 427 3.30e-36 139
MS.gene38703.t1 AT5G41040 28.851 409 240 16 63 451 63 440 3.35e-36 139
MS.gene38703.t1 AT5G41040 28.851 409 240 16 63 451 79 456 4.64e-36 139
MS.gene38703.t1 AT5G63560 28.967 397 237 15 63 444 55 421 1.66e-30 122
MS.gene38703.t1 AT3G48720 31.613 310 176 11 42 339 40 325 2.56e-29 119
MS.gene38703.t1 AT5G57840 27.155 464 249 20 29 451 24 439 3.11e-27 113
MS.gene38703.t1 AT2G40230 27.089 395 237 14 39 414 35 397 4.79e-25 107
MS.gene38703.t1 AT1G31490 27.371 369 211 17 55 401 48 381 7.95e-22 97.8
MS.gene38703.t1 AT1G03390 26.525 377 235 15 54 415 64 413 3.76e-21 96.3
MS.gene38703.t1 AT3G29635 24.885 434 285 14 29 440 29 443 1.53e-20 94.4
MS.gene38703.t1 AT1G27620 26.781 351 218 10 69 404 58 384 6.83e-20 92.0
MS.gene38703.t1 AT1G65450 29.195 298 172 14 63 340 66 344 9.99e-20 91.7
MS.gene38703.t1 AT1G03940 23.659 410 258 15 56 427 53 445 6.34e-19 89.4
MS.gene38703.t1 AT1G78990 25.135 370 207 15 67 404 65 396 2.86e-17 84.3
MS.gene38703.t1 AT3G29590 25.393 382 244 13 77 435 69 432 5.06e-17 83.6
MS.gene38703.t1 AT1G03495 27.451 306 179 15 56 331 53 345 8.10e-17 82.0
MS.gene38703.t1 AT3G03480 28.750 320 181 18 43 342 46 338 2.61e-16 81.3
MS.gene38703.t1 AT5G16410 24.384 406 249 16 67 444 89 464 3.10e-16 81.3
MS.gene38703.t1 AT1G32910 24.595 370 214 15 64 401 60 396 4.50e-16 80.5
MS.gene38703.t1 AT5G39090 24.235 425 217 17 29 402 28 398 4.97e-16 80.5
MS.gene38703.t1 AT1G28680 27.152 302 191 12 72 362 65 348 5.16e-16 80.1
MS.gene38703.t1 AT1G28680 27.152 302 191 12 72 362 65 348 6.08e-16 80.1
MS.gene38703.t1 AT1G24420 25.486 463 283 19 9 452 8 427 2.29e-15 78.2
MS.gene38703.t1 AT1G24420 25.486 463 283 19 9 452 8 427 2.29e-15 78.2
MS.gene38703.t1 AT3G26040 25.990 404 248 18 66 451 56 426 2.50e-15 78.2
MS.gene38703.t1 AT2G25150 24.407 295 191 8 69 349 67 343 6.30e-15 77.0
MS.gene38703.t1 AT5G61160 23.853 436 261 18 46 446 41 440 2.85e-14 75.1
MS.gene38703.t1 AT4G15390 26.398 322 195 14 7 320 9 296 7.97e-14 73.6
MS.gene38703.t1 AT4G15390 26.398 322 195 14 7 320 9 296 7.97e-14 73.6
MS.gene38703.t1 AT5G39050 23.476 328 195 12 155 443 149 459 1.06e-13 73.2
MS.gene38703.t1 AT5G47980 24.797 246 157 7 68 309 60 281 3.87e-12 68.6
MS.gene38703.t1 AT5G23970 25.122 410 254 18 56 450 46 417 7.27e-12 67.4

Find 107 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCGACGGCACCTCTTTC+TGG 0.148559 3.3:-84355472 MS.gene38703:CDS
TTCCATTTCTATCCTCTTTC+CGG 0.165652 3.3:-84355767 MS.gene38703:CDS
ATGAAATCTTGTTGGTTATT+TGG 0.171092 3.3:+84355836 None:intergenic
AATATGTATGGGAATGAATT+TGG 0.211414 3.3:-84354825 MS.gene38703:CDS
CCTTTGCCACAAGAGTATTT+TGG 0.259472 3.3:-84355065 MS.gene38703:CDS
AGCCTGTTAAATGACAAATT+TGG 0.273490 3.3:+84355927 None:intergenic
AACCAAATTTGTCATTTAAC+AGG 0.283464 3.3:-84355929 MS.gene38703:CDS
ATTTGGTTTGAATCTTCAAT+AGG 0.293125 3.3:+84355944 None:intergenic
TGTTTGAAGGGAAGGTTAAT+AGG 0.322567 3.3:+84355332 None:intergenic
CGTCGCCAATACAGTGGTTC+AGG 0.323308 3.3:+84355486 None:intergenic
ACTGAAAGTAAAGGCGAATA+AGG 0.331646 3.3:-84355228 MS.gene38703:CDS
AGATTTCATTGAGAATCTAT+TGG 0.350242 3.3:-84355822 MS.gene38703:CDS
GGAATTGCTTCAGAATAATC+TGG 0.355550 3.3:-84355003 MS.gene38703:CDS
TGAATCTTGCACCGGGGTTA+TGG 0.358731 3.3:+84355681 None:intergenic
CAATAGACTCCGGTGCAAAA+TGG 0.366621 3.3:+84355255 None:intergenic
TTTGGTTTGAATCTTCAATA+GGG 0.367279 3.3:+84355945 None:intergenic
TCACCAAGGTTCAATATGTA+TGG 0.368741 3.3:-84354837 MS.gene38703:CDS
AGGCTTTATCAGCACATGTT+TGG 0.374339 3.3:-84355175 MS.gene38703:CDS
GATTCTCAATGAAATCTTGT+TGG 0.378418 3.3:+84355828 None:intergenic
ATCAACGAAAATAGTATAAG+TGG 0.381161 3.3:+84355712 None:intergenic
ACTTGACACTGTGATACTTT+GGG 0.394360 3.3:+84354860 None:intergenic
GTGAAAAGGTAGACGCGAAA+TGG 0.400218 3.3:+84355385 None:intergenic
AACTTGACACTGTGATACTT+TGG 0.402547 3.3:+84354859 None:intergenic
CCCTCATTGCATTAGGTGTA+AGG 0.407739 3.3:+84354697 None:intergenic
TATAAATAATCGACTAAGAT+TGG 0.409859 3.3:-84355093 MS.gene38703:CDS
TCCAACGCCCTCATTGCATT+AGG 0.417797 3.3:+84354690 None:intergenic
ATTTATAGCTAACTTACAAC+TGG 0.418010 3.3:+84355109 None:intergenic
TCTATGCACTATATCCAAAA+AGG 0.418792 3.3:-84355887 MS.gene38703:CDS
TGAAAAGGTAGACGCGAAAT+GGG 0.427390 3.3:+84355386 None:intergenic
TGGTTATTTGGTGAAGTTGT+TGG 0.431090 3.3:+84355848 None:intergenic
TGAAACTCTTTGAGCGATAG+AGG 0.433956 3.3:+84355991 None:intergenic
CACGATCGTTGGTTTCTACA+AGG 0.434379 3.3:-84355365 MS.gene38703:CDS
AAATTTGTCATTTAACAGGC+TGG 0.441677 3.3:-84355925 MS.gene38703:CDS
GGATATGCAAACAAATTTGA+TGG 0.446592 3.3:-84354774 MS.gene38703:CDS
TGTTCCCTGAACCACTGTAT+TGG 0.458386 3.3:-84355491 MS.gene38703:CDS
TGAGCATGGAAGCATAGATT+TGG 0.460362 3.3:-84354727 MS.gene38703:CDS
AAACTCCTCATGATGTTTGA+AGG 0.463035 3.3:+84355319 None:intergenic
CGTTGGTTTCTACAAGGTTA+CGG 0.463520 3.3:-84355359 MS.gene38703:CDS
GGTAATGTATCATCATATCA+AGG 0.467204 3.3:-84354753 MS.gene38703:CDS
CCAAAATACTCTTGTGGCAA+AGG 0.474802 3.3:+84355065 None:intergenic
ACAATTGGAGGAACATCAAC+CGG 0.476837 3.3:+84355617 None:intergenic
GGCCGGAAAGAGGATAGAAA+TGG 0.482201 3.3:+84355765 None:intergenic
CGGAGAGAGGATATCAGATA+TGG 0.489115 3.3:+84355637 None:intergenic
AAAGAGGATAGAAATGGAAA+AGG 0.493445 3.3:+84355771 None:intergenic
CGACCGAGTTGGTATACAAC+AGG 0.497202 3.3:+84354894 None:intergenic
CCTCATGATGTTTGAAGGGA+AGG 0.506492 3.3:+84355324 None:intergenic
AAGAATCTATCTCTCAAGTT+CGG 0.508887 3.3:+84355278 None:intergenic
ATGGAAAAGGGCATGAGAGA+GGG 0.517142 3.3:+84355784 None:intergenic
TTCCCATACATATTGAACCT+TGG 0.519386 3.3:+84354834 None:intergenic
AATGGAAAAGGGCATGAGAG+AGG 0.522599 3.3:+84355783 None:intergenic
TCTGGGATGGGCAGCATGGA+AGG 0.526844 3.3:-84354985 MS.gene38703:CDS
TGGGTAAAGCGATAGCAGTT+CGG 0.531426 3.3:-84354800 MS.gene38703:CDS
TGCATAGATGAATCTTGCAC+CGG 0.532803 3.3:+84355673 None:intergenic
TGCTTCAGAATAATCTGGGA+TGG 0.534999 3.3:-84354998 MS.gene38703:CDS
AATTCAAGTCACAGAGCTAG+TGG 0.536764 3.3:-84355531 MS.gene38703:CDS
AATTTGTCATTTAACAGGCT+GGG 0.542942 3.3:-84355924 MS.gene38703:CDS
GAATCTTCAATAGGGTGTAG+TGG 0.543868 3.3:+84355953 None:intergenic
AGAAACCAACGATCGTGAAA+AGG 0.544715 3.3:+84355371 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.545045 3.3:-84354819 MS.gene38703:CDS
ACCTAATGCAATGAGGGCGT+TGG 0.546046 3.3:-84354691 MS.gene38703:CDS
TATTGCAAAACTGAAAGTAA+AGG 0.547981 3.3:-84355237 MS.gene38703:CDS
CATATCTGATATCCTCTCTC+CGG 0.547986 3.3:-84355636 MS.gene38703:CDS
GTGACAAGACGGCCGGAAAG+AGG 0.554301 3.3:+84355755 None:intergenic
AAGAGGATAGAAATGGAAAA+GGG 0.559581 3.3:+84355772 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3.3:-84354836 MS.gene38703:CDS
AACTCCTCATGATGTTTGAA+GGG 0.567259 3.3:+84355320 None:intergenic
GTCGCCAATACAGTGGTTCA+GGG 0.567364 3.3:+84355487 None:intergenic
CGAAAATAGTATAAGTGGGA+GGG 0.567849 3.3:+84355717 None:intergenic
GAATTGCTTCAGAATAATCT+GGG 0.575502 3.3:-84355002 MS.gene38703:CDS
GCATAGATGAATCTTGCACC+GGG 0.575886 3.3:+84355674 None:intergenic
AAATACTCTTGTGGCAAAGG+TGG 0.579415 3.3:+84355068 None:intergenic
TCATATCAAGGGTATGAGCA+TGG 0.589477 3.3:-84354741 MS.gene38703:CDS
CCTTACACCTAATGCAATGA+GGG 0.589516 3.3:-84354697 MS.gene38703:CDS
TTTGTGAGTGACAAGACGGC+CGG 0.593267 3.3:+84355748 None:intergenic
CCTTCCCTTCAAACATCATG+AGG 0.593883 3.3:-84355324 MS.gene38703:CDS
GTATGGGAATGAATTTGGAA+TGG 0.594042 3.3:-84354820 MS.gene38703:CDS
TCAACGAAAATAGTATAAGT+GGG 0.595520 3.3:+84355713 None:intergenic
CCTCATTGCATTAGGTGTAA+GGG 0.599303 3.3:+84354698 None:intergenic
GCTTCAGAATAATCTGGGAT+GGG 0.600304 3.3:-84354997 MS.gene38703:CDS
CTGAACCACTGTATTGGCGA+CGG 0.605637 3.3:-84355485 MS.gene38703:CDS
CTTGACACTGTGATACTTTG+GGG 0.607315 3.3:+84354861 None:intergenic
GATCAAAGTGGCGACCGAGT+TGG 0.615671 3.3:+84354883 None:intergenic
ATAATCTGGGATGGGCAGCA+TGG 0.616155 3.3:-84354989 MS.gene38703:CDS
CCCTTACACCTAATGCAATG+AGG 0.616533 3.3:-84354698 MS.gene38703:CDS
GTGTTTCCAAAATACTCTTG+TGG 0.617301 3.3:+84355059 None:intergenic
CAAGTCACAGAGCTAGTGGA+TGG 0.621329 3.3:-84355527 MS.gene38703:CDS
ACGAAAATAGTATAAGTGGG+AGG 0.621520 3.3:+84355716 None:intergenic
GTAATGTATCATCATATCAA+GGG 0.623652 3.3:-84354752 MS.gene38703:CDS
ATGGAAGGTTAATGTGGCTG+TGG 0.624262 3.3:-84354970 MS.gene38703:CDS
GAGGAACATCAACCGGAGAG+AGG 0.629237 3.3:+84355624 None:intergenic
TTGAAGGGAAGGTTAATAGG+TGG 0.632790 3.3:+84355335 None:intergenic
TGATACTTTGGGGATCAAAG+TGG 0.645918 3.3:+84354871 None:intergenic
TCTCCTGTTGTATACCAACT+CGG 0.651878 3.3:-84354897 MS.gene38703:CDS
AGATGTGTCACACGTGCACG+CGG 0.655089 3.3:-84355152 MS.gene38703:CDS
GTGACTTGAATTGATAACAA+CGG 0.655100 3.3:+84355542 None:intergenic
GGCAGCATGGAAGGTTAATG+TGG 0.663013 3.3:-84354976 MS.gene38703:CDS
TAGTGCAGAAACGACTGTGG+AGG 0.677510 3.3:-84355024 MS.gene38703:CDS
AGGTGCCGTCGCCAATACAG+TGG 0.683782 3.3:+84355480 None:intergenic
GGTTAGTGCAGAAACGACTG+TGG 0.691168 3.3:-84355027 MS.gene38703:CDS
TTGAATTGATAACAACGGCA+TGG 0.701648 3.3:+84355547 None:intergenic
GATTGCGGTAACCATAACCC+CGG 0.707381 3.3:-84355692 MS.gene38703:CDS
AAAGCGATAGCAGTTCGGAG+TGG 0.708530 3.3:-84354795 MS.gene38703:CDS
AACAAAGCAATCAACCACGA+CGG 0.711672 3.3:-84355575 MS.gene38703:CDS
GAGGGCGTTGGAATTAGACG+AGG 0.733168 3.3:-84354679 MS.gene38703:CDS
ACGGCATGGTGTGACCGTCG+TGG 0.733894 3.3:+84355561 None:intergenic
TCACAGTGTCAAGTTCACCA+AGG 0.739939 3.3:-84354851 MS.gene38703:CDS
CATAGATGAATCTTGCACCG+GGG 0.755506 3.3:+84355675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTGGAATTTTTTTAATATA+TGG - chr3.3:84355200-84355219 MS.gene38703:CDS 15.0%
!! ATTAAAAAAATTCCAGAAAG+AGG + chr3.3:84355197-84355216 None:intergenic 20.0%
!! TATAAATAATCGACTAAGAT+TGG - chr3.3:84355561-84355580 MS.gene38703:CDS 20.0%
!!! TTTTGAATAATAGACCTTTT+TGG + chr3.3:84354784-84354803 None:intergenic 20.0%
! AACAAAAATATCTTCGTTTC+AGG - chr3.3:84355459-84355478 MS.gene38703:CDS 25.0%
! AATATGTATGGGAATGAATT+TGG - chr3.3:84355829-84355848 MS.gene38703:CDS 25.0%
! AGATTTCATTGAGAATCTAT+TGG - chr3.3:84354832-84354851 MS.gene38703:CDS 25.0%
! ATCAACGAAAATAGTATAAG+TGG + chr3.3:84354945-84354964 None:intergenic 25.0%
! ATTTATAGCTAACTTACAAC+TGG + chr3.3:84355548-84355567 None:intergenic 25.0%
! GTAATGTATCATCATATCAA+GGG - chr3.3:84355902-84355921 MS.gene38703:CDS 25.0%
! TATTGCAAAACTGAAAGTAA+AGG - chr3.3:84355417-84355436 MS.gene38703:CDS 25.0%
! TCAAAAAATGACTCAACAAT+TGG + chr3.3:84355055-84355074 None:intergenic 25.0%
! TCAACGAAAATAGTATAAGT+GGG + chr3.3:84354944-84354963 None:intergenic 25.0%
!! AACCAAATTTGTCATTTAAC+AGG - chr3.3:84354725-84354744 MS.gene38703:CDS 25.0%
!! ATGAAATCTTGTTGGTTATT+TGG + chr3.3:84354821-84354840 None:intergenic 25.0%
!!! ATTTGGTTTGAATCTTCAAT+AGG + chr3.3:84354713-84354732 None:intergenic 25.0%
!!! TATACTATTTTCGTTGATTG+CGG - chr3.3:84354947-84354966 MS.gene38703:CDS 25.0%
!!! TTTGGTTTGAATCTTCAATA+GGG + chr3.3:84354712-84354731 None:intergenic 25.0%
AAAAATGACTCAACAATTGG+AGG + chr3.3:84355052-84355071 None:intergenic 30.0%
AAAGAGGATAGAAATGGAAA+AGG + chr3.3:84354886-84354905 None:intergenic 30.0%
AAGAATCTATCTCTCAAGTT+CGG + chr3.3:84355379-84355398 None:intergenic 30.0%
AAGAGGATAGAAATGGAAAA+GGG + chr3.3:84354885-84354904 None:intergenic 30.0%
AGCCTGTTAAATGACAAATT+TGG + chr3.3:84354730-84354749 None:intergenic 30.0%
GAATTGCTTCAGAATAATCT+GGG - chr3.3:84355652-84355671 MS.gene38703:CDS 30.0%
GATTCTCAATGAAATCTTGT+TGG + chr3.3:84354829-84354848 None:intergenic 30.0%
GGATATGCAAACAAATTTGA+TGG - chr3.3:84355880-84355899 MS.gene38703:CDS 30.0%
GGTAATGTATCATCATATCA+AGG - chr3.3:84355901-84355920 MS.gene38703:CDS 30.0%
TCTATGCACTATATCCAAAA+AGG - chr3.3:84354767-84354786 MS.gene38703:CDS 30.0%
! AAATTTGTCATTTAACAGGC+TGG - chr3.3:84354729-84354748 MS.gene38703:CDS 30.0%
! AATTTGTCATTTAACAGGCT+GGG - chr3.3:84354730-84354749 MS.gene38703:CDS 30.0%
! GTGACTTGAATTGATAACAA+CGG + chr3.3:84355115-84355134 None:intergenic 30.0%
! TATGGGAATGAATTTGGAAT+GGG - chr3.3:84355835-84355854 MS.gene38703:CDS 30.0%
AAACTCCTCATGATGTTTGA+AGG + chr3.3:84355338-84355357 None:intergenic 35.0%
AACTCCTCATGATGTTTGAA+GGG + chr3.3:84355337-84355356 None:intergenic 35.0%
AACTTGACACTGTGATACTT+TGG + chr3.3:84355798-84355817 None:intergenic 35.0%
ACGAAAATAGTATAAGTGGG+AGG + chr3.3:84354941-84354960 None:intergenic 35.0%
ACTGAAAGTAAAGGCGAATA+AGG - chr3.3:84355426-84355445 MS.gene38703:CDS 35.0%
ACTTGACACTGTGATACTTT+GGG + chr3.3:84355797-84355816 None:intergenic 35.0%
CACCAAGGTTCAATATGTAT+GGG - chr3.3:84355818-84355837 MS.gene38703:CDS 35.0%
CGAAAATAGTATAAGTGGGA+GGG + chr3.3:84354940-84354959 None:intergenic 35.0%
GGAATTGCTTCAGAATAATC+TGG - chr3.3:84355651-84355670 MS.gene38703:CDS 35.0%
GTATGGGAATGAATTTGGAA+TGG - chr3.3:84355834-84355853 MS.gene38703:CDS 35.0%
GTGTTTCCAAAATACTCTTG+TGG + chr3.3:84355598-84355617 None:intergenic 35.0%
TCACCAAGGTTCAATATGTA+TGG - chr3.3:84355817-84355836 MS.gene38703:CDS 35.0%
TGCGAAAGATGCTGAAAAAA+TGG - chr3.3:84355728-84355747 MS.gene38703:CDS 35.0%
TGTTTGAAGGGAAGGTTAAT+AGG + chr3.3:84355325-84355344 None:intergenic 35.0%
TTCCATTTCTATCCTCTTTC+CGG - chr3.3:84354887-84354906 MS.gene38703:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + chr3.3:84355823-84355842 None:intergenic 35.0%
! TAGATTCTTCCATTTTGCAC+CGG - chr3.3:84355390-84355409 MS.gene38703:CDS 35.0%
! TGGTTATTTGGTGAAGTTGT+TGG + chr3.3:84354809-84354828 None:intergenic 35.0%
! TTCAGTTTTGCAATAGACTC+CGG + chr3.3:84355412-84355431 None:intergenic 35.0%
! TTGAATTGATAACAACGGCA+TGG + chr3.3:84355110-84355129 None:intergenic 35.0%
AAATACTCTTGTGGCAAAGG+TGG + chr3.3:84355589-84355608 None:intergenic 40.0%
AACAAAGCAATCAACCACGA+CGG - chr3.3:84355079-84355098 MS.gene38703:CDS 40.0%
AATTCAAGTCACAGAGCTAG+TGG - chr3.3:84355123-84355142 MS.gene38703:CDS 40.0%
ACAATTGGAGGAACATCAAC+CGG + chr3.3:84355040-84355059 None:intergenic 40.0%
AGAAACCAACGATCGTGAAA+AGG + chr3.3:84355286-84355305 None:intergenic 40.0%
CATATCTGATATCCTCTCTC+CGG - chr3.3:84355018-84355037 MS.gene38703:CDS 40.0%
CCAAAATACTCTTGTGGCAA+AGG + chr3.3:84355592-84355611 None:intergenic 40.0%
CCTCATTGCATTAGGTGTAA+GGG + chr3.3:84355959-84355978 None:intergenic 40.0%
CCTTACACCTAATGCAATGA+GGG - chr3.3:84355957-84355976 MS.gene38703:CDS 40.0%
CTTGACACTGTGATACTTTG+GGG + chr3.3:84355796-84355815 None:intergenic 40.0%
GAATCTTCAATAGGGTGTAG+TGG + chr3.3:84354704-84354723 None:intergenic 40.0%
TCTCCTGTTGTATACCAACT+CGG - chr3.3:84355757-84355776 MS.gene38703:CDS 40.0%
TGAAAAGGTAGACGCGAAAT+GGG + chr3.3:84355271-84355290 None:intergenic 40.0%
TGAAACTCTTTGAGCGATAG+AGG + chr3.3:84354666-84354685 None:intergenic 40.0%
TGCATAGATGAATCTTGCAC+CGG + chr3.3:84354984-84355003 None:intergenic 40.0%
TGCTTCAGAATAATCTGGGA+TGG - chr3.3:84355656-84355675 MS.gene38703:CDS 40.0%
TTGAAGGGAAGGTTAATAGG+TGG + chr3.3:84355322-84355341 None:intergenic 40.0%
! AGGCTTTATCAGCACATGTT+TGG - chr3.3:84355479-84355498 MS.gene38703:CDS 40.0%
! GCTTCAGAATAATCTGGGAT+GGG - chr3.3:84355657-84355676 MS.gene38703:CDS 40.0%
! TCATATCAAGGGTATGAGCA+TGG - chr3.3:84355913-84355932 MS.gene38703:CDS 40.0%
! TGAGCATGGAAGCATAGATT+TGG - chr3.3:84355927-84355946 MS.gene38703:CDS 40.0%
! TGATACTTTGGGGATCAAAG+TGG + chr3.3:84355786-84355805 None:intergenic 40.0%
!! CGTTGGTTTCTACAAGGTTA+CGG - chr3.3:84355295-84355314 MS.gene38703:CDS 40.0%
!! CTAGTGGATGGTGTTTTCAT+AGG - chr3.3:84355139-84355158 MS.gene38703:CDS 40.0%
!! GAGTTTTGTGAGTGACAAGA+CGG + chr3.3:84354913-84354932 None:intergenic 40.0%
!! TTTTGGAAACACAGTTGACG+TGG - chr3.3:84355606-84355625 MS.gene38703:CDS 40.0%
!!! CCTTTGCCACAAGAGTATTT+TGG - chr3.3:84355589-84355608 MS.gene38703:CDS 40.0%
ATGGAAGGTTAATGTGGCTG+TGG - chr3.3:84355684-84355703 MS.gene38703:CDS 45.0%
CAATAGACTCCGGTGCAAAA+TGG + chr3.3:84355402-84355421 None:intergenic 45.0%
CATAGATGAATCTTGCACCG+GGG + chr3.3:84354982-84355001 None:intergenic 45.0%
CCCTCATTGCATTAGGTGTA+AGG + chr3.3:84355960-84355979 None:intergenic 45.0%
CCCTTACACCTAATGCAATG+AGG - chr3.3:84355956-84355975 MS.gene38703:CDS 45.0%
CCTCATGATGTTTGAAGGGA+AGG + chr3.3:84355333-84355352 None:intergenic 45.0%
CCTTCCCTTCAAACATCATG+AGG - chr3.3:84355330-84355349 MS.gene38703:CDS 45.0%
CGGAGAGAGGATATCAGATA+TGG + chr3.3:84355020-84355039 None:intergenic 45.0%
GCATAGATGAATCTTGCACC+GGG + chr3.3:84354983-84355002 None:intergenic 45.0%
GTGAAAAGGTAGACGCGAAA+TGG + chr3.3:84355272-84355291 None:intergenic 45.0%
TCACAGTGTCAAGTTCACCA+AGG - chr3.3:84355803-84355822 MS.gene38703:CDS 45.0%
TGGGTAAAGCGATAGCAGTT+CGG - chr3.3:84355854-84355873 MS.gene38703:CDS 45.0%
TGTTCCCTGAACCACTGTAT+TGG - chr3.3:84355163-84355182 MS.gene38703:CDS 45.0%
! AATGGAAAAGGGCATGAGAG+AGG + chr3.3:84354874-84354893 None:intergenic 45.0%
! ATGGAAAAGGGCATGAGAGA+GGG + chr3.3:84354873-84354892 None:intergenic 45.0%
! GTCTACCTTTTCACGATCGT+TGG - chr3.3:84355278-84355297 MS.gene38703:CDS 45.0%
!! CACGATCGTTGGTTTCTACA+AGG - chr3.3:84355289-84355308 MS.gene38703:CDS 45.0%
AAAGCGATAGCAGTTCGGAG+TGG - chr3.3:84355859-84355878 MS.gene38703:CDS 50.0%
CAAGTCACAGAGCTAGTGGA+TGG - chr3.3:84355127-84355146 MS.gene38703:CDS 50.0%
GATTGCGGTAACCATAACCC+CGG - chr3.3:84354962-84354981 MS.gene38703:CDS 50.0%
GGCAGCATGGAAGGTTAATG+TGG - chr3.3:84355678-84355697 MS.gene38703:CDS 50.0%
GGCCGGAAAGAGGATAGAAA+TGG + chr3.3:84354892-84354911 None:intergenic 50.0%
GGTTAGTGCAGAAACGACTG+TGG - chr3.3:84355627-84355646 MS.gene38703:CDS 50.0%
GTCGCCAATACAGTGGTTCA+GGG + chr3.3:84355170-84355189 None:intergenic 50.0%
TAGTGCAGAAACGACTGTGG+AGG - chr3.3:84355630-84355649 MS.gene38703:CDS 50.0%
TCCAACGCCCTCATTGCATT+AGG + chr3.3:84355967-84355986 None:intergenic 50.0%
TGAATCTTGCACCGGGGTTA+TGG + chr3.3:84354976-84354995 None:intergenic 50.0%
TTTGTGAGTGACAAGACGGC+CGG + chr3.3:84354909-84354928 None:intergenic 50.0%
! ACCTAATGCAATGAGGGCGT+TGG - chr3.3:84355963-84355982 MS.gene38703:CDS 50.0%
! ATAATCTGGGATGGGCAGCA+TGG - chr3.3:84355665-84355684 MS.gene38703:CDS 50.0%
! CTGAACCACTGTATTGGCGA+CGG - chr3.3:84355169-84355188 MS.gene38703:CDS 50.0%
!! CGACCGAGTTGGTATACAAC+AGG + chr3.3:84355763-84355782 None:intergenic 50.0%
AGATGTGTCACACGTGCACG+CGG - chr3.3:84355502-84355521 MS.gene38703:CDS 55.0%
CGTCGCCAATACAGTGGTTC+AGG + chr3.3:84355171-84355190 None:intergenic 55.0%
GAGGAACATCAACCGGAGAG+AGG + chr3.3:84355033-84355052 None:intergenic 55.0%
GATCAAAGTGGCGACCGAGT+TGG + chr3.3:84355774-84355793 None:intergenic 55.0%
!! GAGGGCGTTGGAATTAGACG+AGG - chr3.3:84355975-84355994 MS.gene38703:CDS 55.0%
GTGACAAGACGGCCGGAAAG+AGG + chr3.3:84354902-84354921 None:intergenic 60.0%
TTGGCGACGGCACCTCTTTC+TGG - chr3.3:84355182-84355201 MS.gene38703:CDS 60.0%
! AGGTGCCGTCGCCAATACAG+TGG + chr3.3:84355177-84355196 None:intergenic 60.0%
! TCTGGGATGGGCAGCATGGA+AGG - chr3.3:84355669-84355688 MS.gene38703:CDS 60.0%
ACGGCATGGTGTGACCGTCG+TGG + chr3.3:84355096-84355115 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 84354656 84356020 84354656 ID=MS.gene38703
chr3.3 mRNA 84354656 84356020 84354656 ID=MS.gene38703.t1;Parent=MS.gene38703
chr3.3 exon 84354656 84356020 84354656 ID=MS.gene38703.t1.exon1;Parent=MS.gene38703.t1
chr3.3 CDS 84354656 84356020 84354656 ID=cds.MS.gene38703.t1;Parent=MS.gene38703.t1
Gene Sequence

>MS.gene38703

ATGACTTCCTCTATCGCTCAAAGAGTTTCAGAATGTTTTATCAAACCACTACACCCTATTGAAGATTCAAACCAAATTTGTCATTTAACAGGCTGGGATATTGCTATGTCATCTATGCACTATATCCAAAAAGGTCTATTATTCAAAAAACCAACAACTTCACCAAATAACCAACAAGATTTCATTGAGAATCTATTGGAGAAGCTTAAACACTCCCTCTCTCATGCCCTTTTCCATTTCTATCCTCTTTCCGGCCGTCTTGTCACTCACAAAACTCAAAACCCTCCCACTTATACTATTTTCGTTGATTGCGGTAACCATAACCCCGGTGCAAGATTCATCTATGCAACTGCAAACGTGACCATATCTGATATCCTCTCTCCGGTTGATGTTCCTCCAATTGTTGAGTCATTTTTTGATCTCAACAAAGCAATCAACCACGACGGTCACACCATGCCGTTGTTATCAATTCAAGTCACAGAGCTAGTGGATGGTGTTTTCATAGGTTGTTCCCTGAACCACTGTATTGGCGACGGCACCTCTTTCTGGAATTTTTTTAATATATGGTCTGAGATATTTCAATCTCAAAGTTGTGAAAATGAAAACGATGTTCCCATTTCGCGTCTACCTTTTCACGATCGTTGGTTTCTACAAGGTTACGGTCCACCTATTAACCTTCCCTTCAAACATCATGAGGAGTTTATCAGCAGATATGAATCTCCGAACTTGAGAGATAGATTCTTCCATTTTGCACCGGAGTCTATTGCAAAACTGAAAGTAAAGGCGAATAAGGAGTTTAACACAACAAAAATATCTTCGTTTCAGGCTTTATCAGCACATGTTTGGAGATGTGTCACACGTGCACGCGGACTAACAAATGATCGAAAAACCAGTTGTAAGTTAGCTATAAATAATCGACTAAGATTGGAACCACCTTTGCCACAAGAGTATTTTGGAAACACAGTTGACGTGGTTAGTGCAGAAACGACTGTGGAGGAATTGCTTCAGAATAATCTGGGATGGGCAGCATGGAAGGTTAATGTGGCTGTGGTGAATCATGACGATAAAGAAGTGCGAAAGATGCTGAAAAAATGGTTAGAGTCTCCTGTTGTATACCAACTCGGTCGCCACTTTGATCCCCAAAGTATCACAGTGTCAAGTTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGTAAAGCGATAGCAGTTCGGAGTGGATATGCAAACAAATTTGATGGTAATGTATCATCATATCAAGGGTATGAGCATGGAAGCATAGATTTGGCGTTGACCCTTACACCTAATGCAATGAGGGCGTTGGAATTAGACGAGGAGTTCATGAATACTGTAGTTTGA
Protein sequence

>MS.gene38703.t1

MTSSIAQRVSECFIKPLHPIEDSNQICHLTGWDIAMSSMHYIQKGLLFKKPTTSPNNQQDFIENLLEKLKHSLSHALFHFYPLSGRLVTHKTQNPPTYTIFVDCGNHNPGARFIYATANVTISDILSPVDVPPIVESFFDLNKAINHDGHTMPLLSIQVTELVDGVFIGCSLNHCIGDGTSFWNFFNIWSEIFQSQSCENENDVPISRLPFHDRWFLQGYGPPINLPFKHHEEFISRYESPNLRDRFFHFAPESIAKLKVKANKEFNTTKISSFQALSAHVWRCVTRARGLTNDRKTSCKLAINNRLRLEPPLPQEYFGNTVDVVSAETTVEELLQNNLGWAAWKVNVAVVNHDDKEVRKMLKKWLESPVVYQLGRHFDPQSITVSSSPRFNMYGNEFGMGKAIAVRSGYANKFDGNVSSYQGYEHGSIDLALTLTPNAMRALELDEEFMNTVV