AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012965

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012965.t1 MTR_3g101900 94.272 419 18 1 1 419 37 449 0.0 819
MS.gene012965.t1 MTR_3g101930 71.496 421 113 3 1 417 37 454 0.0 632
MS.gene012965.t1 MTR_3g102020 67.294 425 129 3 1 421 36 454 0.0 585
MS.gene012965.t1 MTR_3g101960 67.548 416 126 3 1 413 36 445 0.0 580
MS.gene012965.t1 MTR_3g101970 65.721 423 121 3 1 419 36 438 0.0 572
MS.gene012965.t1 MTR_3g102010 66.274 424 134 4 1 421 37 454 0.0 566
MS.gene012965.t1 MTR_3g102030 57.407 324 106 4 48 371 29 320 1.63e-126 369
MS.gene012965.t1 MTR_4g094842 40.909 418 222 11 8 414 41 444 1.81e-93 289
MS.gene012965.t1 MTR_4g094835 40.334 419 218 10 7 414 45 442 3.58e-91 283
MS.gene012965.t1 MTR_8g041770 38.902 419 227 10 8 418 44 441 7.09e-91 282
MS.gene012965.t1 MTR_3g088420 39.474 418 224 10 8 418 38 433 5.74e-90 280
MS.gene012965.t1 MTR_4g022800 38.771 423 229 12 8 418 38 442 1.30e-89 279
MS.gene012965.t1 MTR_4g015180 37.155 471 226 9 1 418 34 487 1.37e-89 280
MS.gene012965.t1 MTR_3g070170 37.363 455 226 10 1 419 40 471 2.96e-89 279
MS.gene012965.t1 MTR_4g022720 39.277 415 229 9 9 418 39 435 2.80e-88 275
MS.gene012965.t1 MTR_4g022825 39.671 426 219 12 8 418 38 440 2.88e-88 275
MS.gene012965.t1 MTR_4g022670 38.679 424 227 12 8 418 38 441 5.10e-87 272
MS.gene012965.t1 MTR_0190s0020 38.313 415 237 8 8 418 43 442 1.17e-86 271
MS.gene012965.t1 MTR_4g094838 37.321 418 228 6 4 414 38 428 8.85e-85 266
MS.gene012965.t1 MTR_7g026860 37.352 423 231 12 8 418 38 438 2.27e-82 260
MS.gene012965.t1 MTR_5g081960 35.792 461 237 11 1 414 34 482 4.97e-82 261
MS.gene012965.t1 MTR_4g094832 40.341 352 194 5 68 414 86 426 1.25e-79 253
MS.gene012965.t1 MTR_3g026310 37.500 432 226 12 9 418 39 448 1.87e-77 248
MS.gene012965.t1 MTR_4g094828 36.427 431 241 11 4 419 39 451 9.25e-75 241
MS.gene012965.t1 MTR_4g022290 35.377 424 237 11 7 416 43 443 8.42e-74 238
MS.gene012965.t1 MTR_4g022760 37.500 352 194 8 8 348 38 374 7.27e-69 224
MS.gene012965.t1 MTR_8g079710 35.663 415 231 16 7 407 42 434 5.69e-64 213
MS.gene012965.t1 MTR_6g045553 31.461 445 255 11 6 417 47 474 7.31e-58 197
MS.gene012965.t1 MTR_6g016815 32.911 395 183 9 27 418 66 381 5.01e-52 179
MS.gene012965.t1 MTR_2g064495 29.167 432 240 17 5 408 33 426 3.64e-40 149
MS.gene012965.t1 MTR_4g007540 28.972 428 247 18 5 408 33 427 3.74e-40 149
MS.gene012965.t1 MTR_8g075610 28.019 414 253 14 11 408 30 414 6.42e-40 148
MS.gene012965.t1 MTR_2g105330 29.398 432 239 18 5 408 33 426 1.61e-39 147
MS.gene012965.t1 MTR_8g040780 33.745 243 149 6 186 422 1 237 9.04e-39 140
MS.gene012965.t1 MTR_7g026850 41.509 159 84 4 8 165 21 171 2.96e-32 121
MS.gene012965.t1 MTR_3g098980 27.877 391 243 13 30 408 66 429 6.54e-32 126
MS.gene012965.t1 MTR_8g096530 30.573 314 184 11 24 324 53 345 1.07e-31 125
MS.gene012965.t1 MTR_7g026770 38.798 183 86 3 118 300 132 288 1.49e-31 122
MS.gene012965.t1 MTR_3g049150 25.962 416 268 12 12 405 40 437 2.70e-28 116
MS.gene012965.t1 MTR_3g026950 23.721 430 256 15 4 393 32 429 2.15e-27 114
MS.gene012965.t1 MTR_6g034495 43.548 124 65 3 41 161 45 166 5.09e-26 103
MS.gene012965.t1 MTR_8g040760 37.297 185 102 5 8 191 41 212 1.27e-25 104
MS.gene012965.t1 MTR_4g127650 24.943 437 275 16 1 408 37 449 9.54e-25 106
MS.gene012965.t1 MTR_4g094825 36.667 150 84 3 269 418 253 391 6.05e-24 103
MS.gene012965.t1 MTR_4g094825 38.562 153 89 2 4 155 102 250 9.90e-23 99.8
MS.gene012965.t1 MTR_7g114520 27.098 417 247 19 25 415 61 446 5.47e-23 101
MS.gene012965.t1 MTR_7g085990 27.483 433 257 17 12 408 39 450 1.50e-22 100
MS.gene012965.t1 MTR_7g085930 27.064 436 247 16 12 404 43 450 2.75e-22 99.4
MS.gene012965.t1 MTR_7g086000 27.189 434 254 16 12 408 42 450 3.18e-22 99.0
MS.gene012965.t1 MTR_4g097090 25.298 419 257 13 12 399 47 440 7.83e-22 97.8
MS.gene012965.t1 MTR_3g071320 26.891 357 219 10 29 367 59 391 1.96e-21 96.3
MS.gene012965.t1 MTR_5g076700 25.666 413 257 18 25 415 60 444 1.99e-21 96.7
MS.gene012965.t1 MTR_4g013050 24.079 407 236 13 28 413 51 405 2.59e-20 92.8
MS.gene012965.t1 MTR_6g015815 24.824 427 274 12 7 407 47 452 4.38e-20 92.8
MS.gene012965.t1 MTR_1g040195 26.933 375 232 15 23 386 33 376 4.45e-20 92.4
MS.gene012965.t1 MTR_5g026320 25.522 431 252 20 12 413 58 448 8.51e-20 91.7
MS.gene012965.t1 MTR_7g084940 27.119 354 215 12 28 366 67 392 1.28e-19 91.3
MS.gene012965.t1 MTR_1g081660 26.368 402 256 15 25 410 68 445 3.05e-19 90.1
MS.gene012965.t1 MTR_6g034485 35.433 127 75 2 173 295 2 125 7.08e-19 82.8
MS.gene012965.t1 MTR_6g053810 27.241 290 193 10 30 312 71 349 3.06e-18 87.0
MS.gene012965.t1 MTR_1g040175 27.049 366 227 17 25 378 59 396 5.88e-18 86.3
MS.gene012965.t1 MTR_5g090880 24.021 383 235 13 30 380 67 425 4.26e-17 83.6
MS.gene012965.t1 MTR_3g112180 25.281 356 224 13 27 365 58 388 5.54e-17 83.2
MS.gene012965.t1 MTR_4g055170 26.132 287 179 9 30 302 69 336 1.18e-16 82.4
MS.gene012965.t1 MTR_6g015850 24.466 421 280 13 7 407 47 449 1.79e-16 81.6
MS.gene012965.t1 MTR_5g090830 22.938 388 246 11 11 367 47 412 2.28e-16 81.3
MS.gene012965.t1 MTR_7g026830 45.783 83 42 2 63 142 15 97 6.11e-16 73.6
MS.gene012965.t1 MTR_3g465980 26.087 322 185 15 5 302 42 334 1.02e-15 79.3
MS.gene012965.t1 MTR_8g079970 25.185 405 250 19 5 391 36 405 1.16e-15 79.0
MS.gene012965.t1 MTR_5g090810 22.680 388 247 11 11 367 47 412 2.21e-15 78.2
MS.gene012965.t1 MTR_6g015830 23.168 423 266 12 17 407 57 452 2.87e-15 77.8
MS.gene012965.t1 MTR_2g089650 23.095 433 266 20 6 399 41 445 6.49e-15 77.0
MS.gene012965.t1 MTR_7g114490 25.616 406 249 18 25 408 60 434 1.77e-14 75.5
MS.gene012965.t1 MTR_7g114960 25.826 333 223 11 4 331 37 350 9.15e-14 73.2
MS.gene012965.t1 MTR_3g465930 26.224 286 181 9 30 302 69 337 3.14e-13 71.6
MS.gene012965.t1 MTR_3g450690 21.791 335 208 12 24 331 66 373 4.60e-13 71.2
MS.gene012965.t1 MTR_3g465910 25.795 283 178 10 30 299 68 331 1.40e-12 69.7
MS.gene012965.t1 MTR_3g049140 23.645 203 130 3 210 393 27 223 1.91e-12 67.4
MS.gene012965.t1 MTR_7g084910 25.651 269 167 8 28 282 68 317 4.05e-12 68.2
MS.gene012965.t1 MTR_8g079950 26.455 378 225 17 11 369 41 384 6.20e-12 67.8
MS.gene012965.t1 MTR_2g089670 22.222 396 247 12 7 366 59 429 1.72e-11 66.2
MS.gene012965.t1 MTR_2g015430 24.000 350 231 11 33 370 61 387 1.88e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012965.t1 AT5G42830 54.415 419 186 3 1 419 37 450 2.95e-159 457
MS.gene012965.t1 AT5G07850 52.370 422 191 4 1 418 38 453 1.18e-150 436
MS.gene012965.t1 AT5G07870 51.402 428 194 4 1 418 38 461 5.30e-146 424
MS.gene012965.t1 AT5G07860 52.619 420 183 5 3 417 40 448 6.91e-144 418
MS.gene012965.t1 AT2G39980 39.400 467 212 9 1 418 36 480 3.42e-98 302
MS.gene012965.t1 AT5G67150 40.235 425 227 11 6 420 41 448 2.24e-95 294
MS.gene012965.t1 AT5G01210 37.719 456 226 10 1 418 34 469 1.07e-85 270
MS.gene012965.t1 AT3G50270 36.768 427 245 10 4 420 39 450 6.07e-81 257
MS.gene012965.t1 AT3G50280 37.470 419 238 11 4 414 37 439 2.44e-80 255
MS.gene012965.t1 AT3G50300 35.663 415 241 11 4 407 37 436 4.20e-74 239
MS.gene012965.t1 AT5G67160 34.906 424 237 12 6 420 41 434 5.32e-71 231
MS.gene012965.t1 AT5G38130 35.572 402 235 12 6 400 59 443 6.07e-70 229
MS.gene012965.t1 AT5G23940 33.489 427 251 9 6 414 37 448 2.05e-65 218
MS.gene012965.t1 AT3G50280 38.245 319 181 7 102 414 1 309 1.13e-60 200
MS.gene012965.t1 AT3G50290 38.214 280 149 7 93 364 6 269 8.23e-50 170
MS.gene012965.t1 AT5G48930 29.551 423 235 16 13 408 41 427 1.34e-40 150
MS.gene012965.t1 AT5G41040 27.851 377 233 12 24 387 63 413 1.27e-31 125
MS.gene012965.t1 AT5G41040 27.851 377 233 12 24 387 79 429 1.82e-31 125
MS.gene012965.t1 AT2G19070 27.149 442 260 16 1 408 31 444 1.11e-30 123
MS.gene012965.t1 AT3G48720 28.653 349 214 12 30 367 61 385 1.44e-26 111
MS.gene012965.t1 AT5G63560 28.342 374 225 15 7 368 40 382 9.47e-26 108
MS.gene012965.t1 AT2G40230 26.757 370 218 13 29 376 58 396 1.30e-21 97.1
MS.gene012965.t1 AT3G26040 25.728 412 250 15 27 419 56 430 1.29e-20 94.0
MS.gene012965.t1 AT1G03940 23.934 422 286 15 11 408 47 457 1.93e-20 94.0
MS.gene012965.t1 AT1G65450 26.823 384 230 17 4 366 47 400 2.78e-18 87.0
MS.gene012965.t1 AT1G28680 26.243 362 207 15 33 370 65 390 9.86e-18 85.5
MS.gene012965.t1 AT1G28680 26.214 309 181 11 33 322 65 345 2.50e-17 84.0
MS.gene012965.t1 AT1G78990 24.110 365 219 14 28 367 65 396 4.98e-16 80.5
MS.gene012965.t1 AT5G07080 25.828 302 202 12 4 297 44 331 1.20e-15 79.0
MS.gene012965.t1 AT1G31490 25.420 417 260 18 16 413 48 432 1.35e-14 75.9
MS.gene012965.t1 AT1G03495 25.633 316 186 14 11 294 47 345 1.36e-14 75.1
MS.gene012965.t1 AT3G30280 22.782 417 272 16 24 419 50 437 2.44e-13 72.0
MS.gene012965.t1 AT1G24420 23.711 388 254 15 41 419 76 430 3.21e-13 71.6
MS.gene012965.t1 AT1G24420 23.711 388 254 15 41 419 76 430 3.21e-13 71.6
MS.gene012965.t1 AT5G39080 23.077 442 276 15 4 407 38 453 8.21e-13 70.5
MS.gene012965.t1 AT1G32910 23.770 366 224 16 28 367 63 399 1.30e-12 69.7
MS.gene012965.t1 AT5G39090 22.824 425 278 15 7 407 41 439 6.64e-12 67.4
MS.gene012965.t1 AT1G65450 27.165 254 151 9 129 366 1 236 1.88e-11 65.1

Find 104 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCATCAAAGTGGTGTTTA+AGG 0.177579 3.4:+89733964 None:intergenic
AACATGTATGGAAATGAATT+TGG 0.200204 3.4:-89733909 MS.gene012965:CDS
AAATTTCATTGATGATCTTT+TGG 0.244067 3.4:-89734912 MS.gene012965:CDS
TTCCATTTCTATCCTTTATC+TGG 0.285102 3.4:-89734857 MS.gene012965:CDS
TTGGTGATGGAACTTCTTAT+TGG 0.296229 3.4:-89734562 MS.gene012965:CDS
AAACAGCATTCATGAAATTC+TGG 0.315247 3.4:+89733742 None:intergenic
TGTTCTATGAATCACTATAT+TGG 0.320492 3.4:-89734581 MS.gene012965:CDS
CTCTTCCACAAGAGTACTTT+GGG 0.331112 3.4:-89734148 MS.gene012965:CDS
AGGAGGAGGGAGCATGGATT+TGG 0.335716 3.4:-89733808 MS.gene012965:CDS
TTTGCCATCTTTCGTACCTC+TGG 0.340501 3.4:+89734017 None:intergenic
GGAAGAGGTGGTTTCATTCT+CGG 0.346910 3.4:+89734164 None:intergenic
TGAATTGCTAGAGAATGATT+TGG 0.359006 3.4:-89734087 MS.gene012965:CDS
TCACCAAGGTTTAACATGTA+TGG 0.363014 3.4:-89733921 MS.gene012965:CDS
GCTGAAAGCTAAGGCCAATA+TGG 0.363379 3.4:-89734312 MS.gene012965:CDS
CGTTGGTTTCCAGAAGGTTA+TGG 0.364442 3.4:-89734443 MS.gene012965:CDS
ATCAGTGGACCATAACCTTC+TGG 0.372215 3.4:+89734434 None:intergenic
AGTCATTATCAGCACATGTT+TGG 0.378371 3.4:-89734259 MS.gene012965:CDS
ACAAGTTGTTACTTGATCAT+TGG 0.401455 3.4:+89734208 None:intergenic
ACAACTGATGGAACATCAAT+TGG 0.410907 3.4:+89734707 None:intergenic
GTTTGTATTAGACTCCATAT+TGG 0.414390 3.4:+89734298 None:intergenic
CCTCTTCCACAAGAGTACTT+TGG 0.419393 3.4:-89734149 MS.gene012965:CDS
TGTACCCCATCTACTTCAAA+AGG 0.420856 3.4:-89733693 MS.gene012965:CDS
TGTGATGAGAGCACTAGAAT+TGG 0.432754 3.4:-89733766 MS.gene012965:CDS
TCTAGTGCTCTCATCACATC+TGG 0.439173 3.4:+89733771 None:intergenic
TATGGAAATGAATTTGGAAT+GGG 0.439212 3.4:-89733903 MS.gene012965:CDS
ACAGATATAATTAGAGAGAT+TGG 0.441305 3.4:+89733713 None:intergenic
AACAATGACTTAACAACTGA+TGG 0.441689 3.4:+89734695 None:intergenic
GAATTGCTAGAGAATGATTT+GGG 0.452968 3.4:-89734086 MS.gene012965:CDS
GTAACAAGACGACCAGATAA+AGG 0.453295 3.4:+89734845 None:intergenic
TTGCCATCTTTCGTACCTCT+GGG 0.453949 3.4:+89734018 None:intergenic
GACCAGATAAAGGATAGAAA+TGG 0.453990 3.4:+89734855 None:intergenic
TTCGTACCTCTGGGTCATCA+TGG 0.471436 3.4:+89734027 None:intergenic
CCAAAGTACTCTTGTGGAAG+AGG 0.473388 3.4:+89734149 None:intergenic
TGTTTAAGGCTTAATATCAT+AGG 0.479366 3.4:+89733978 None:intergenic
CTTAAACACCACTTTGATGC+TGG 0.486430 3.4:-89733963 MS.gene012965:CDS
ATTGGAATTTCTTTAATACA+TGG 0.493830 3.4:-89734544 MS.gene012965:CDS
AAAGAATCTCTCTCTCAGCT+TGG 0.494857 3.4:+89734361 None:intergenic
ACATGAAGGAGGAGGGAGCA+TGG 0.495499 3.4:-89733814 MS.gene012965:CDS
ATCTCGTCATGATGTTTGAA+TGG 0.499005 3.4:+89734404 None:intergenic
ACGAAAATATTATAAGTAGG+AGG 0.500048 3.4:+89734806 None:intergenic
AAGAATCTCTCTCTCAGCTT+GGG 0.501298 3.4:+89734362 None:intergenic
TTTCCATACATGTTAAACCT+TGG 0.506348 3.4:+89733918 None:intergenic
TGCTAGAGAATGATTTGGGA+TGG 0.507254 3.4:-89734082 MS.gene012965:CDS
ATGGAATAGAGCAAGAGAGA+GGG 0.512197 3.4:+89734874 None:intergenic
TGGAAACCAACGATTGTGAA+AGG 0.513895 3.4:+89734454 None:intergenic
CATCCACTAGCTCAGTGACT+TGG 0.518092 3.4:+89734618 None:intergenic
GCCAACCCTTTCACAATCGT+TGG 0.519850 3.4:-89734460 MS.gene012965:CDS
TCAACGAAAATATTATAAGT+AGG 0.529867 3.4:+89734803 None:intergenic
TGGGAATGTGACAGCATATG+AGG 0.531079 3.4:-89733838 MS.gene012965:CDS
ATGAATCACTATATTGGTGA+TGG 0.531436 3.4:-89734575 MS.gene012965:CDS
GGAAACCAACGATTGTGAAA+GGG 0.532567 3.4:+89734455 None:intergenic
AGTAACAACTTGTATGTTAG+CGG 0.536386 3.4:-89734198 MS.gene012965:CDS
TGGGCAGCATGGAAGGTGCA+TGG 0.536391 3.4:-89734062 MS.gene012965:CDS
TATCCAAGTCACTGAGCTAG+TGG 0.536796 3.4:-89734621 MS.gene012965:CDS
TGACCCAGAGGTACGAAAGA+TGG 0.540305 3.4:-89734021 MS.gene012965:CDS
TTTGGGATGGGCAGCATGGA+AGG 0.543018 3.4:-89734069 MS.gene012965:CDS
CACAATCGTTGGTTTCCAGA+AGG 0.545576 3.4:-89734449 MS.gene012965:CDS
TAACCACTCAAAACTGCCAC+TGG 0.548632 3.4:+89733876 None:intergenic
TATGAGGGACATGAAGGAGG+AGG 0.555088 3.4:-89733822 MS.gene012965:CDS
GGTTATGCAAACAAATGTGA+TGG 0.557612 3.4:-89733858 MS.gene012965:CDS
CGAAAATATTATAAGTAGGA+GGG 0.558814 3.4:+89734807 None:intergenic
GTATGGAAATGAATTTGGAA+TGG 0.561756 3.4:-89733904 MS.gene012965:CDS
TAGATTCATTCATGCAACAT+TGG 0.562632 3.4:-89734756 MS.gene012965:CDS
AATCTCTCTCTCAGCTTGGG+TGG 0.563254 3.4:+89734365 None:intergenic
AATGGAATAGAGCAAGAGAG+AGG 0.567357 3.4:+89734873 None:intergenic
TCTATGAACTATATCCAGAA+AGG 0.569526 3.4:-89734965 MS.gene012965:CDS
TGCATGAATGAATCTAGCTC+CGG 0.570333 3.4:+89734763 None:intergenic
ATTCTCTAGCAATTCACCTA+CGG 0.570871 3.4:+89734094 None:intergenic
TCTGAAATATTTCAAGCTCA+AGG 0.572514 3.4:-89734521 MS.gene012965:CDS
GCTAGAGAATGATTTGGGAT+GGG 0.574242 3.4:-89734081 MS.gene012965:CDS
TGCACTTACTGTGTCAACTG+AGG 0.579536 3.4:+89734124 None:intergenic
TTTCTGGATATAGTTCATAG+AGG 0.580769 3.4:+89734967 None:intergenic
ACAGCATATGAGGGACATGA+AGG 0.583593 3.4:-89733828 MS.gene012965:CDS
GTACCCCATCTACTTCAAAA+GGG 0.585527 3.4:-89733692 MS.gene012965:CDS
ATGATTTGGGATGGGCAGCA+TGG 0.587560 3.4:-89734073 MS.gene012965:CDS
ATATTTCAAGCTCAAGGTCA+AGG 0.592438 3.4:-89734515 MS.gene012965:CDS
TATTGCAAAGCTGAAAGCTA+AGG 0.595205 3.4:-89734321 MS.gene012965:CDS
CAAGGTCAAGGTCAAGGCCA+AGG 0.597402 3.4:-89734503 MS.gene012965:CDS
AAGTACTCTTGTGGAAGAGG+TGG 0.603445 3.4:+89734152 None:intergenic
GGTGTCATGATATCAGATAC+TGG 0.604513 3.4:+89734728 None:intergenic
AAATGCGAACATCATGACCT+TGG 0.608455 3.4:+89734486 None:intergenic
CAAGCTCAAGGTCAAGGTCA+AGG 0.616290 3.4:-89734509 MS.gene012965:CDS
GATATAGTTCATAGAGGACA+TGG 0.617037 3.4:+89734973 None:intergenic
CAAGTCACTGAGCTAGTGGA+TGG 0.621922 3.4:-89734617 MS.gene012965:CDS
GATTGTAGTAACAATAACTC+CGG 0.623849 3.4:-89734782 MS.gene012965:CDS
GTTATGCAAACAAATGTGAT+GGG 0.628176 3.4:-89733857 MS.gene012965:CDS
ATGAGGGACATGAAGGAGGA+GGG 0.631723 3.4:-89733821 MS.gene012965:CDS
CCACACTACCAGCATCAAAG+TGG 0.631844 3.4:+89733955 None:intergenic
TTATCAGCACATGTTTGGAG+AGG 0.638377 3.4:-89734254 MS.gene012965:CDS
CCACTTTGATGCTGGTAGTG+TGG 0.651666 3.4:-89733955 MS.gene012965:CDS
GCATATGAGGGACATGAAGG+AGG 0.651988 3.4:-89733825 MS.gene012965:CDS
ACCAACGATTGTGAAAGGGT+TGG 0.664079 3.4:+89734459 None:intergenic
TTGGATAGATAACAACGGCA+TGG 0.667589 3.4:+89734637 None:intergenic
GGGAATGTGACAGCATATGA+GGG 0.668225 3.4:-89733837 MS.gene012965:CDS
ATTTGGAATGGGGAAACCAG+TGG 0.673938 3.4:-89733892 MS.gene012965:CDS
ATGGAAATGAATTTGGAATG+GGG 0.678734 3.4:-89733902 MS.gene012965:CDS
GAGGTCCCAAAGTACTCTTG+TGG 0.680293 3.4:+89734143 None:intergenic
AGAGGTATAACTCATGCTCG+CGG 0.707970 3.4:-89734236 MS.gene012965:CDS
TTGAATGGAAGATTGATCAG+TGG 0.709801 3.4:+89734419 None:intergenic
ATCATGACGAGATTATAACT+AGG 0.713806 3.4:-89734394 MS.gene012965:CDS
TGGCAATGATAAGTTCACCA+AGG 0.714004 3.4:-89733935 MS.gene012965:CDS
AATAAAGCAATCAATCACGA+CGG 0.729010 3.4:-89734665 MS.gene012965:CDS
AGCAAACCATGATGACCCAG+AGG 0.741285 3.4:-89734033 MS.gene012965:CDS
GTGACTTGGATAGATAACAA+CGG 0.742107 3.4:+89734632 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGGAATTTCTTTAATACA+TGG - chr3.4:89734113-89734132 MS.gene012965:CDS 20.0%
!! TCAACGAAAATATTATAAGT+AGG + chr3.4:89733857-89733876 None:intergenic 20.0%
!!! AAATTTCATTGATGATCTTT+TGG - chr3.4:89733745-89733764 MS.gene012965:CDS 20.0%
!!! ATGAAATTTTGTTGGTTATT+TGG + chr3.4:89733734-89733753 None:intergenic 20.0%
!!! TGAAATTTTGTTGGTTATTT+GGG + chr3.4:89733733-89733752 None:intergenic 20.0%
! AACATGTATGGAAATGAATT+TGG - chr3.4:89734748-89734767 MS.gene012965:CDS 25.0%
! ACAGATATAATTAGAGAGAT+TGG + chr3.4:89734947-89734966 None:intergenic 25.0%
! ACGAAAATATTATAAGTAGG+AGG + chr3.4:89733854-89733873 None:intergenic 25.0%
! CGAAAATATTATAAGTAGGA+GGG + chr3.4:89733853-89733872 None:intergenic 25.0%
! TGTTCTATGAATCACTATAT+TGG - chr3.4:89734076-89734095 MS.gene012965:CDS 25.0%
!! GATCATCAATGAAATTTTGT+TGG + chr3.4:89733742-89733761 None:intergenic 25.0%
!! TATGGAAATGAATTTGGAAT+GGG - chr3.4:89734754-89734773 MS.gene012965:CDS 25.0%
!! TGTTTAAGGCTTAATATCAT+AGG + chr3.4:89734682-89734701 None:intergenic 25.0%
!!! TATTTGGGTTTTTGAATAAG+AGG + chr3.4:89733718-89733737 None:intergenic 25.0%
AAACAGCATTCATGAAATTC+TGG + chr3.4:89734918-89734937 None:intergenic 30.0%
AACAATGACTTAACAACTGA+TGG + chr3.4:89733965-89733984 None:intergenic 30.0%
AATAAAGCAATCAATCACGA+CGG - chr3.4:89733992-89734011 MS.gene012965:CDS 30.0%
AGTAACAACTTGTATGTTAG+CGG - chr3.4:89734459-89734478 MS.gene012965:CDS 30.0%
ATCATGACGAGATTATAACT+AGG - chr3.4:89734263-89734282 MS.gene012965:CDS 30.0%
ATGAATCACTATATTGGTGA+TGG - chr3.4:89734082-89734101 MS.gene012965:CDS 30.0%
GAATTGCTAGAGAATGATTT+GGG - chr3.4:89734571-89734590 MS.gene012965:CDS 30.0%
GATTGTAGTAACAATAACTC+CGG - chr3.4:89733875-89733894 MS.gene012965:CDS 30.0%
GTATGGAAATGAATTTGGAA+TGG - chr3.4:89734753-89734772 MS.gene012965:CDS 30.0%
GTTATGCAAACAAATGTGAT+GGG - chr3.4:89734800-89734819 MS.gene012965:CDS 30.0%
GTTTGTATTAGACTCCATAT+TGG + chr3.4:89734362-89734381 None:intergenic 30.0%
TAGATTCATTCATGCAACAT+TGG - chr3.4:89733901-89733920 MS.gene012965:CDS 30.0%
TCTATGAACTATATCCAGAA+AGG - chr3.4:89733692-89733711 MS.gene012965:CDS 30.0%
TGAATTGCTAGAGAATGATT+TGG - chr3.4:89734570-89734589 MS.gene012965:CDS 30.0%
TTCCATTTCTATCCTTTATC+TGG - chr3.4:89733800-89733819 MS.gene012965:CDS 30.0%
TTTCCATACATGTTAAACCT+TGG + chr3.4:89734742-89734761 None:intergenic 30.0%
TTTCTGGATATAGTTCATAG+AGG + chr3.4:89733693-89733712 None:intergenic 30.0%
! ACAAGTTGTTACTTGATCAT+TGG + chr3.4:89734452-89734471 None:intergenic 30.0%
! ATGGAAATGAATTTGGAATG+GGG - chr3.4:89734755-89734774 MS.gene012965:CDS 30.0%
! TCTGAAATATTTCAAGCTCA+AGG - chr3.4:89734136-89734155 MS.gene012965:CDS 30.0%
!!! ATAACCCTTTTGAAGTAGAT+GGG + chr3.4:89734972-89734991 None:intergenic 30.0%
!!! TATAACCCTTTTGAAGTAGA+TGG + chr3.4:89734973-89734992 None:intergenic 30.0%
ACAACTGATGGAACATCAAT+TGG + chr3.4:89733953-89733972 None:intergenic 35.0%
AGTCATTATCAGCACATGTT+TGG - chr3.4:89734398-89734417 MS.gene012965:CDS 35.0%
ATATTTCAAGCTCAAGGTCA+AGG - chr3.4:89734142-89734161 MS.gene012965:CDS 35.0%
ATCTCGTCATGATGTTTGAA+TGG + chr3.4:89734256-89734275 None:intergenic 35.0%
ATTCTCTAGCAATTCACCTA+CGG + chr3.4:89734566-89734585 None:intergenic 35.0%
GACCAGATAAAGGATAGAAA+TGG + chr3.4:89733805-89733824 None:intergenic 35.0%
GGTTATGCAAACAAATGTGA+TGG - chr3.4:89734799-89734818 MS.gene012965:CDS 35.0%
TACGAAAGATGGCAAAAAAG+TGG - chr3.4:89734647-89734666 MS.gene012965:CDS 35.0%
TATTGCAAAGCTGAAAGCTA+AGG - chr3.4:89734336-89734355 MS.gene012965:CDS 35.0%
TCACCAAGGTTTAACATGTA+TGG - chr3.4:89734736-89734755 MS.gene012965:CDS 35.0%
TTGAATGGAAGATTGATCAG+TGG + chr3.4:89734241-89734260 None:intergenic 35.0%
! GTGACTTGGATAGATAACAA+CGG + chr3.4:89734028-89734047 None:intergenic 35.0%
! TTTTGAATAAGAGGCCTTTC+TGG + chr3.4:89733709-89733728 None:intergenic 35.0%
!! TTGGTGATGGAACTTCTTAT+TGG - chr3.4:89734095-89734114 MS.gene012965:CDS 35.0%
!!! TAACCCTTTTGAAGTAGATG+GGG + chr3.4:89734971-89734990 None:intergenic 35.0%
AAAGAATCTCTCTCTCAGCT+TGG + chr3.4:89734299-89734318 None:intergenic 40.0%
AAATGCGAACATCATGACCT+TGG + chr3.4:89734174-89734193 None:intergenic 40.0%
AAGAATCTCTCTCTCAGCTT+GGG + chr3.4:89734298-89734317 None:intergenic 40.0%
AATGGAATAGAGCAAGAGAG+AGG + chr3.4:89733787-89733806 None:intergenic 40.0%
ATGGAATAGAGCAAGAGAGA+GGG + chr3.4:89733786-89733805 None:intergenic 40.0%
CAGCATCAAAGTGGTGTTTA+AGG + chr3.4:89734696-89734715 None:intergenic 40.0%
CTTAAACACCACTTTGATGC+TGG - chr3.4:89734694-89734713 MS.gene012965:CDS 40.0%
GGAAACCAACGATTGTGAAA+GGG + chr3.4:89734205-89734224 None:intergenic 40.0%
GTAACAAGACGACCAGATAA+AGG + chr3.4:89733815-89733834 None:intergenic 40.0%
GTAAGTGCAAAAACAACCGT+AGG - chr3.4:89734547-89734566 MS.gene012965:CDS 40.0%
GTACCCCATCTACTTCAAAA+GGG - chr3.4:89734965-89734984 MS.gene012965:CDS 40.0%
TGGAAACCAACGATTGTGAA+AGG + chr3.4:89734206-89734225 None:intergenic 40.0%
TGGCAATGATAAGTTCACCA+AGG - chr3.4:89734722-89734741 MS.gene012965:CDS 40.0%
TGTACCCCATCTACTTCAAA+AGG - chr3.4:89734964-89734983 MS.gene012965:CDS 40.0%
TTATCAGCACATGTTTGGAG+AGG - chr3.4:89734403-89734422 MS.gene012965:CDS 40.0%
TTGGATAGATAACAACGGCA+TGG + chr3.4:89734023-89734042 None:intergenic 40.0%
! GCTAGAGAATGATTTGGGAT+GGG - chr3.4:89734576-89734595 MS.gene012965:CDS 40.0%
! GGTGTCATGATATCAGATAC+TGG + chr3.4:89733932-89733951 None:intergenic 40.0%
! TGCATGAATGAATCTAGCTC+CGG + chr3.4:89733897-89733916 None:intergenic 40.0%
! TGCTAGAGAATGATTTGGGA+TGG - chr3.4:89734575-89734594 MS.gene012965:CDS 40.0%
!! CTAGTGGATGGTGTTTTCAT+AGG - chr3.4:89734052-89734071 MS.gene012965:CDS 40.0%
!! CTCTTCCACAAGAGTACTTT+GGG - chr3.4:89734509-89734528 MS.gene012965:CDS 40.0%
!! TGTGATGAGAGCACTAGAAT+TGG - chr3.4:89734891-89734910 MS.gene012965:CDS 40.0%
AAGTACTCTTGTGGAAGAGG+TGG + chr3.4:89734508-89734527 None:intergenic 45.0%
ACAGCATATGAGGGACATGA+AGG - chr3.4:89734829-89734848 MS.gene012965:CDS 45.0%
ACCAACGATTGTGAAAGGGT+TGG + chr3.4:89734201-89734220 None:intergenic 45.0%
ATCAGTGGACCATAACCTTC+TGG + chr3.4:89734226-89734245 None:intergenic 45.0%
GCTGAAAGCTAAGGCCAATA+TGG - chr3.4:89734345-89734364 MS.gene012965:CDS 45.0%
GGAAGAGGTGGTTTCATTCT+CGG + chr3.4:89734496-89734515 None:intergenic 45.0%
GGGAATGTGACAGCATATGA+GGG - chr3.4:89734820-89734839 MS.gene012965:CDS 45.0%
TAACCACTCAAAACTGCCAC+TGG + chr3.4:89734784-89734803 None:intergenic 45.0%
TATCCAAGTCACTGAGCTAG+TGG - chr3.4:89734036-89734055 MS.gene012965:CDS 45.0%
TCTAGTGCTCTCATCACATC+TGG + chr3.4:89734889-89734908 None:intergenic 45.0%
TGCACTTACTGTGTCAACTG+AGG + chr3.4:89734536-89734555 None:intergenic 45.0%
TGGGAATGTGACAGCATATG+AGG - chr3.4:89734819-89734838 MS.gene012965:CDS 45.0%
TTGCCATCTTTCGTACCTCT+GGG + chr3.4:89734642-89734661 None:intergenic 45.0%
TTTGCCATCTTTCGTACCTC+TGG + chr3.4:89734643-89734662 None:intergenic 45.0%
! AGAGGTATAACTCATGCTCG+CGG - chr3.4:89734421-89734440 MS.gene012965:CDS 45.0%
! ATTTGGAATGGGGAAACCAG+TGG - chr3.4:89734765-89734784 MS.gene012965:CDS 45.0%
! CCAAAGTACTCTTGTGGAAG+AGG + chr3.4:89734511-89734530 None:intergenic 45.0%
!! CACAATCGTTGGTTTCCAGA+AGG - chr3.4:89734208-89734227 MS.gene012965:CDS 45.0%
!! CCTCTTCCACAAGAGTACTT+TGG - chr3.4:89734508-89734527 MS.gene012965:CDS 45.0%
!! CGTTGGTTTCCAGAAGGTTA+TGG - chr3.4:89734214-89734233 MS.gene012965:CDS 45.0%
!!! AAACCAGTGGCAGTTTTGAG+TGG - chr3.4:89734778-89734797 MS.gene012965:CDS 45.0%
AATCTCTCTCTCAGCTTGGG+TGG + chr3.4:89734295-89734314 None:intergenic 50.0%
AGCAAACCATGATGACCCAG+AGG - chr3.4:89734624-89734643 MS.gene012965:CDS 50.0%
ATGAGGGACATGAAGGAGGA+GGG - chr3.4:89734836-89734855 MS.gene012965:CDS 50.0%
CAAGCTCAAGGTCAAGGTCA+AGG - chr3.4:89734148-89734167 MS.gene012965:CDS 50.0%
CAAGTCACTGAGCTAGTGGA+TGG - chr3.4:89734040-89734059 MS.gene012965:CDS 50.0%
CATCCACTAGCTCAGTGACT+TGG + chr3.4:89734042-89734061 None:intergenic 50.0%
CCACACTACCAGCATCAAAG+TGG + chr3.4:89734705-89734724 None:intergenic 50.0%
GCATATGAGGGACATGAAGG+AGG - chr3.4:89734832-89734851 MS.gene012965:CDS 50.0%
GCCAACCCTTTCACAATCGT+TGG - chr3.4:89734197-89734216 MS.gene012965:CDS 50.0%
TATGAGGGACATGAAGGAGG+AGG - chr3.4:89734835-89734854 MS.gene012965:CDS 50.0%
TTCGTACCTCTGGGTCATCA+TGG + chr3.4:89734633-89734652 None:intergenic 50.0%
! ATGATTTGGGATGGGCAGCA+TGG - chr3.4:89734584-89734603 MS.gene012965:CDS 50.0%
! CCACTTTGATGCTGGTAGTG+TGG - chr3.4:89734702-89734721 MS.gene012965:CDS 50.0%
! GAGGTCCCAAAGTACTCTTG+TGG + chr3.4:89734517-89734536 None:intergenic 50.0%
! TGACCCAGAGGTACGAAAGA+TGG - chr3.4:89734636-89734655 MS.gene012965:CDS 50.0%
ACATGAAGGAGGAGGGAGCA+TGG - chr3.4:89734843-89734862 MS.gene012965:CDS 55.0%
CAAGGTCAAGGTCAAGGCCA+AGG - chr3.4:89734154-89734173 MS.gene012965:CDS 55.0%
! AGGAGGAGGGAGCATGGATT+TGG - chr3.4:89734849-89734868 MS.gene012965:CDS 55.0%
! TTTGGGATGGGCAGCATGGA+AGG - chr3.4:89734588-89734607 MS.gene012965:CDS 55.0%
! TGGGCAGCATGGAAGGTGCA+TGG - chr3.4:89734595-89734614 MS.gene012965:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89733686 89734993 89733686 ID=MS.gene012965
chr3.4 mRNA 89733686 89734993 89733686 ID=MS.gene012965.t1;Parent=MS.gene012965
chr3.4 exon 89733686 89734993 89733686 ID=MS.gene012965.t1.exon1;Parent=MS.gene012965.t1
chr3.4 CDS 89733686 89734993 89733686 ID=cds.MS.gene012965.t1;Parent=MS.gene012965.t1
Gene Sequence

>MS.gene012965

ATGTCCTCTATGAACTATATCCAGAAAGGCCTCTTATTCAAAAACCCAAATAACCAACAAAATTTCATTGATGATCTTTTGGAGAAGCTCAAACACTCCCTCTCTCTTGCTCTATTCCATTTCTATCCTTTATCTGGTCGTCTTGTTACACAAAAATCTCAAGACCCTCCTACTTATAATATTTTCGTTGATTGTAGTAACAATAACTCCGGAGCTAGATTCATTCATGCAACATTGGATGCACCAGTATCTGATATCATGACACCAATTGATGTTCCATCAGTTGTTAAGTCATTGTTTGATCTCAATAAAGCAATCAATCACGACGGTCACACCATGCCGTTGTTATCTATCCAAGTCACTGAGCTAGTGGATGGTGTTTTCATAGGTTGTTCTATGAATCACTATATTGGTGATGGAACTTCTTATTGGAATTTCTTTAATACATGGTCTGAAATATTTCAAGCTCAAGGTCAAGGTCAAGGCCAAGGTCATGATGTTCGCATTTCACGCCAACCCTTTCACAATCGTTGGTTTCCAGAAGGTTATGGTCCACTGATCAATCTTCCATTCAAACATCATGACGAGATTATAACTAGGTATGAACCACCCAAGCTGAGAGAGAGATTCTTTCATTTTACAGCAGAGTCTATTGCAAAGCTGAAAGCTAAGGCCAATATGGAGTCTAATACAAACAAAATCTCTTCATTTCAGTCATTATCAGCACATGTTTGGAGAGGTATAACTCATGCTCGCGGCTTAACCAATGATCAAGTAACAACTTGTATGTTAGCGGTAAATAATCGACCGAGAATGAAACCACCTCTTCCACAAGAGTACTTTGGGACCTCAGTTGACACAGTAAGTGCAAAAACAACCGTAGGTGAATTGCTAGAGAATGATTTGGGATGGGCAGCATGGAAGGTGCATGGTGCTGTAGCAAACCATGATGACCCAGAGGTACGAAAGATGGCAAAAAAGTGGTTAGAGTCTCCTATGATATTAAGCCTTAAACACCACTTTGATGCTGGTAGTGTGGCAATGATAAGTTCACCAAGGTTTAACATGTATGGAAATGAATTTGGAATGGGGAAACCAGTGGCAGTTTTGAGTGGTTATGCAAACAAATGTGATGGGAATGTGACAGCATATGAGGGACATGAAGGAGGAGGGAGCATGGATTTGGAAATTTCTCTTTCTCCAGATGTGATGAGAGCACTAGAATTGGACCAGAATTTCATGAATGCTGTTTCTGTGACCAATCTCTCTAATTATATCTGTACCCCATCTACTTCAAAAGGGTTATAG
Protein sequence

>MS.gene012965.t1

MSSMNYIQKGLLFKNPNNQQNFIDDLLEKLKHSLSLALFHFYPLSGRLVTQKSQDPPTYNIFVDCSNNNSGARFIHATLDAPVSDIMTPIDVPSVVKSLFDLNKAINHDGHTMPLLSIQVTELVDGVFIGCSMNHYIGDGTSYWNFFNTWSEIFQAQGQGQGQGHDVRISRQPFHNRWFPEGYGPLINLPFKHHDEIITRYEPPKLRERFFHFTAESIAKLKAKANMESNTNKISSFQSLSAHVWRGITHARGLTNDQVTTCMLAVNNRPRMKPPLPQEYFGTSVDTVSAKTTVGELLENDLGWAAWKVHGAVANHDDPEVRKMAKKWLESPMILSLKHHFDAGSVAMISSPRFNMYGNEFGMGKPVAVLSGYANKCDGNVTAYEGHEGGGSMDLEISLSPDVMRALELDQNFMNAVSVTNLSNYICTPSTSKGL