AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene046246

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046246.t1 MTR_3g101970 90.487 452 28 2 1 451 1 438 0.0 847
MS.gene046246.t1 MTR_3g101960 87.865 445 54 0 1 445 1 445 0.0 829
MS.gene046246.t1 MTR_3g102020 84.464 457 70 1 1 456 1 457 0.0 810
MS.gene046246.t1 MTR_3g102010 78.913 460 89 2 1 456 2 457 0.0 753
MS.gene046246.t1 MTR_3g101900 67.253 455 138 4 1 451 2 449 0.0 617
MS.gene046246.t1 MTR_3g101930 66.000 450 139 5 9 449 10 454 0.0 600
MS.gene046246.t1 MTR_3g102030 72.327 318 62 2 86 403 29 320 3.11e-167 473
MS.gene046246.t1 MTR_4g015180 39.286 504 235 11 1 450 1 487 3.30e-97 301
MS.gene046246.t1 MTR_3g070170 38.066 486 227 13 5 446 11 466 1.54e-95 296
MS.gene046246.t1 MTR_5g081960 38.124 501 236 12 1 446 1 482 3.06e-95 296
MS.gene046246.t1 MTR_4g094842 39.301 458 250 11 3 446 1 444 5.12e-94 291
MS.gene046246.t1 MTR_4g022825 38.688 442 244 9 20 450 15 440 5.61e-90 281
MS.gene046246.t1 MTR_8g041770 39.529 425 241 9 29 450 30 441 1.05e-89 280
MS.gene046246.t1 MTR_4g022800 38.149 443 245 11 21 450 16 442 7.09e-89 278
MS.gene046246.t1 MTR_0190s0020 38.642 427 246 8 28 450 28 442 1.10e-88 277
MS.gene046246.t1 MTR_4g094835 36.980 457 260 10 3 446 1 442 1.12e-86 272
MS.gene046246.t1 MTR_4g022720 39.080 435 245 9 21 450 16 435 1.81e-86 271
MS.gene046246.t1 MTR_3g088420 38.621 435 241 10 23 450 18 433 1.12e-85 269
MS.gene046246.t1 MTR_4g022670 37.868 441 248 9 21 450 16 441 1.33e-85 269
MS.gene046246.t1 MTR_7g026860 36.961 441 249 10 21 450 16 438 3.25e-84 266
MS.gene046246.t1 MTR_4g094838 37.150 428 234 8 28 446 27 428 8.56e-83 262
MS.gene046246.t1 MTR_3g026310 38.182 440 239 11 29 450 24 448 4.64e-82 260
MS.gene046246.t1 MTR_4g094828 36.910 466 252 15 8 451 6 451 2.93e-81 258
MS.gene046246.t1 MTR_4g022290 36.429 420 235 10 23 429 24 424 1.37e-78 251
MS.gene046246.t1 MTR_8g079710 36.830 429 230 14 21 431 21 426 8.63e-73 236
MS.gene046246.t1 MTR_4g094832 38.968 349 198 6 105 446 86 426 6.67e-70 228
MS.gene046246.t1 MTR_4g022760 32.880 441 226 10 21 450 16 397 2.89e-65 215
MS.gene046246.t1 MTR_6g045553 31.405 484 271 12 5 449 13 474 1.74e-61 207
MS.gene046246.t1 MTR_6g016815 31.163 430 214 12 23 450 32 381 6.63e-49 172
MS.gene046246.t1 MTR_8g040780 38.073 218 130 4 240 453 20 236 5.19e-41 147
MS.gene046246.t1 MTR_4g007540 26.444 450 291 15 6 440 3 427 2.98e-34 133
MS.gene046246.t1 MTR_8g075610 28.571 399 228 15 68 440 47 414 2.46e-33 130
MS.gene046246.t1 MTR_2g064495 26.290 407 272 12 48 440 34 426 8.31e-31 123
MS.gene046246.t1 MTR_8g040760 36.866 217 126 6 8 223 6 212 1.08e-30 118
MS.gene046246.t1 MTR_2g105330 26.044 407 273 12 48 440 34 426 1.63e-30 122
MS.gene046246.t1 MTR_7g026850 43.333 150 74 4 42 190 20 159 2.67e-30 116
MS.gene046246.t1 MTR_8g096530 29.841 315 190 11 62 362 53 350 2.51e-28 117
MS.gene046246.t1 MTR_7g026770 37.640 178 90 3 155 332 132 288 5.35e-27 110
MS.gene046246.t1 MTR_4g127650 26.966 445 259 17 19 431 30 440 1.93e-26 111
MS.gene046246.t1 MTR_3g098980 28.852 305 193 10 68 362 66 356 6.88e-26 109
MS.gene046246.t1 MTR_3g049150 27.361 413 234 17 56 431 46 429 2.98e-25 108
MS.gene046246.t1 MTR_1g040175 29.155 367 223 17 65 419 61 402 6.52e-25 107
MS.gene046246.t1 MTR_6g034495 44.915 118 61 3 79 193 45 161 1.16e-24 100
MS.gene046246.t1 MTR_4g094825 38.667 150 81 2 301 450 253 391 7.16e-24 103
MS.gene046246.t1 MTR_4g094825 33.846 195 112 6 29 220 92 272 2.97e-22 98.6
MS.gene046246.t1 MTR_6g053810 31.142 289 175 12 69 344 72 349 8.03e-23 100
MS.gene046246.t1 MTR_5g076700 24.508 457 295 16 2 436 3 431 8.51e-23 100
MS.gene046246.t1 MTR_4g097090 24.444 450 272 17 29 440 30 449 2.53e-22 99.4
MS.gene046246.t1 MTR_3g112180 27.666 347 225 11 65 399 58 390 6.61e-22 98.2
MS.gene046246.t1 MTR_3g026950 24.938 405 250 16 62 431 48 433 4.78e-21 95.5
MS.gene046246.t1 MTR_1g040195 24.762 420 277 16 50 450 21 420 1.03e-20 94.4
MS.gene046246.t1 MTR_7g114550 25.951 447 275 20 30 447 24 443 1.17e-20 94.7
MS.gene046246.t1 MTR_1g040215 27.299 348 216 15 13 347 16 339 2.72e-20 93.6
MS.gene046246.t1 MTR_7g027175 28.840 319 200 11 56 362 56 359 4.28e-20 92.8
MS.gene046246.t1 MTR_1g081660 26.923 390 246 15 65 437 70 437 1.15e-19 91.7
MS.gene046246.t1 MTR_7g014160 25.214 468 293 20 15 441 16 467 1.53e-18 88.2
MS.gene046246.t1 MTR_3g465980 25.852 352 211 14 6 334 10 334 1.63e-18 88.2
MS.gene046246.t1 MTR_1g040315 27.687 307 195 11 30 325 23 313 1.84e-18 87.4
MS.gene046246.t1 MTR_7g086000 26.515 396 243 18 71 433 63 443 2.71e-18 87.4
MS.gene046246.t1 MTR_6g034485 33.065 124 82 1 205 327 2 125 1.26e-17 79.3
MS.gene046246.t1 MTR_3g465990 25.723 346 219 13 6 334 10 334 1.53e-17 85.1
MS.gene046246.t1 MTR_7g114960 25.758 396 269 10 65 446 60 444 2.48e-17 84.3
MS.gene046246.t1 MTR_7g084940 25.524 286 183 10 64 334 65 335 6.72e-17 83.2
MS.gene046246.t1 MTR_4g055170 24.555 281 185 8 68 334 69 336 1.55e-16 82.0
MS.gene046246.t1 MTR_6g015320 25.553 407 252 15 60 436 58 443 1.86e-16 81.6
MS.gene046246.t1 MTR_7g013850 25.000 424 250 16 30 402 30 436 1.98e-16 81.6
MS.gene046246.t1 MTR_4g013050 24.688 401 220 15 66 436 51 399 6.26e-16 79.7
MS.gene046246.t1 MTR_3g465930 27.338 278 176 9 68 331 69 334 8.06e-16 79.7
MS.gene046246.t1 MTR_7g026830 50.000 70 34 1 111 179 28 97 1.61e-15 72.4
MS.gene046246.t1 MTR_6g015850 25.714 420 244 15 60 441 62 451 2.83e-15 78.2
MS.gene046246.t1 MTR_3g049140 26.108 203 134 4 242 431 27 226 1.02e-14 74.3
MS.gene046246.t1 MTR_7g085930 23.333 420 265 15 50 431 45 445 2.67e-14 75.1
MS.gene046246.t1 MTR_3g465910 26.531 294 154 11 69 331 69 331 4.34e-14 74.7
MS.gene046246.t1 MTR_2g015430 24.425 348 224 14 71 401 61 386 1.09e-13 73.2
MS.gene046246.t1 MTR_6g015315 25.120 418 257 15 55 439 50 444 2.46e-13 72.0
MS.gene046246.t1 MTR_3g465950 26.545 275 181 7 69 331 69 334 4.14e-13 71.2
MS.gene046246.t1 MTR_5g090830 22.739 387 241 17 48 399 49 412 5.40e-13 71.2
MS.gene046246.t1 MTR_2g045540 35.227 88 49 1 242 329 27 106 6.03e-13 65.5
MS.gene046246.t1 MTR_7g014610 22.545 448 295 16 29 436 29 464 6.40e-13 70.9
MS.gene046246.t1 MTR_5g090810 22.043 372 235 15 63 399 61 412 2.10e-12 69.3
MS.gene046246.t1 MTR_0502s0010 29.200 250 133 12 111 337 2 230 2.24e-12 67.0
MS.gene046246.t1 MTR_7g014630 30.841 214 136 6 2 209 3 210 3.43e-12 68.2
MS.gene046246.t1 MTR_7g014620 23.585 424 283 18 29 432 15 417 3.45e-12 68.6
MS.gene046246.t1 MTR_7g014200 24.130 460 303 19 15 441 16 462 4.57e-12 68.2
MS.gene046246.t1 MTR_2g089755 21.655 411 271 11 29 402 37 433 4.95e-12 68.2
MS.gene046246.t1 MTR_5g090880 22.911 371 232 16 64 399 63 414 5.42e-12 68.2
MS.gene046246.t1 MTR_7g084910 23.765 324 206 11 18 315 10 318 1.05e-11 67.0
MS.gene046246.t1 MTR_7g056087 22.164 379 249 14 6 361 4 359 1.31e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046246.t1 AT5G42830 55.830 446 181 4 13 451 14 450 0.0 515
MS.gene046246.t1 AT5G07850 53.139 446 208 1 6 450 8 453 9.93e-173 493
MS.gene046246.t1 AT5G07860 53.125 448 199 5 6 449 8 448 2.34e-163 469
MS.gene046246.t1 AT5G07870 50.437 458 210 3 6 450 8 461 2.30e-160 461
MS.gene046246.t1 AT5G67150 39.648 454 251 12 8 451 7 447 1.49e-100 308
MS.gene046246.t1 AT2G39980 39.703 471 216 10 29 450 29 480 5.40e-99 305
MS.gene046246.t1 AT5G01210 38.115 488 243 12 3 450 1 469 4.92e-89 279
MS.gene046246.t1 AT3G50270 36.404 456 264 12 8 451 8 449 2.99e-84 266
MS.gene046246.t1 AT3G50280 35.982 453 267 12 3 446 1 439 1.40e-79 254
MS.gene046246.t1 AT3G50300 35.535 439 260 12 3 432 1 425 6.63e-77 247
MS.gene046246.t1 AT5G38130 35.308 439 253 12 6 432 24 443 1.57e-71 233
MS.gene046246.t1 AT5G67160 35.280 428 251 11 28 451 28 433 2.94e-70 229
MS.gene046246.t1 AT5G23940 33.478 460 277 12 6 448 3 450 6.90e-65 217
MS.gene046246.t1 AT3G50280 38.762 307 177 6 146 446 8 309 2.74e-56 189
MS.gene046246.t1 AT3G50290 37.452 259 149 6 147 396 15 269 5.09e-43 153
MS.gene046246.t1 AT5G48930 26.711 453 286 15 6 440 3 427 6.45e-34 132
MS.gene046246.t1 AT2G19070 27.523 436 258 15 29 431 25 435 1.06e-32 129
MS.gene046246.t1 AT5G41040 28.977 352 217 11 62 399 63 395 9.64e-29 118
MS.gene046246.t1 AT5G41040 28.977 352 217 11 62 399 79 411 1.44e-28 117
MS.gene046246.t1 AT3G48720 29.341 334 193 15 18 334 18 325 1.06e-23 103
MS.gene046246.t1 AT1G31490 27.924 419 246 21 58 455 52 435 2.11e-21 96.7
MS.gene046246.t1 AT1G65450 28.000 300 181 11 56 335 60 344 3.84e-21 95.9
MS.gene046246.t1 AT5G63560 28.025 314 191 12 62 361 55 347 9.72e-20 91.7
MS.gene046246.t1 AT1G28680 26.014 296 190 10 71 353 65 344 1.40e-18 87.8
MS.gene046246.t1 AT1G28680 26.014 296 190 10 71 353 65 344 1.66e-18 88.2
MS.gene046246.t1 AT2G40230 25.926 378 215 14 63 409 54 397 2.68e-18 87.4
MS.gene046246.t1 AT3G26040 25.314 478 280 22 6 454 3 432 3.01e-17 84.3
MS.gene046246.t1 AT1G27620 27.445 317 178 11 68 358 58 348 8.02e-17 82.8
MS.gene046246.t1 AT1G78990 23.948 309 194 12 48 342 52 333 1.41e-16 82.0
MS.gene046246.t1 AT5G16410 23.741 417 260 16 66 455 89 474 1.97e-15 79.0
MS.gene046246.t1 AT3G03480 25.915 328 211 13 30 342 33 343 3.35e-15 77.8
MS.gene046246.t1 AT1G03390 24.570 407 244 18 56 431 67 441 1.32e-14 76.3
MS.gene046246.t1 AT2G25150 26.061 330 210 14 61 374 60 371 3.11e-14 75.1
MS.gene046246.t1 AT1G65450 28.723 188 108 6 166 335 1 180 1.10e-13 71.6
MS.gene046246.t1 AT5G39080 21.712 479 306 17 3 439 2 453 8.64e-11 64.3

Find 106 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCCTAAGGAATATTT+TGG 0.093791 3.4:-9527859 MS.gene046246:CDS
CAAAATGATATTCCTGTTTA+TGG 0.111562 3.4:-9528204 MS.gene046246:CDS
AATACGTATGGAAATGAATT+TGG 0.183687 3.4:-9527619 MS.gene046246:CDS
TTAAATGGAAGGTTGATAAT+TGG 0.249266 3.4:+9528129 None:intergenic
AACTCGTGGTGATGTTTAAA+TGG 0.296206 3.4:+9528114 None:intergenic
AATCTTTATCAGCATTAGTT+TGG 0.307683 3.4:-9527969 MS.gene046246:CDS
CCACTTATCATCACAGTATA+AGG 0.318537 3.4:+9527655 None:intergenic
CACGAGTTTGTTCACACATA+TGG 0.341216 3.4:-9528099 MS.gene046246:CDS
AATGTGTTAAATGACATATT+TGG 0.351013 3.4:+9528706 None:intergenic
ATAATTGGACCATAACCTTC+TGG 0.355852 3.4:+9528144 None:intergenic
TCATGCACGTCGACTACAAC+AGG 0.363744 3.4:-9527935 MS.gene046246:CDS
AACATGCAGAAACGATGTTT+AGG 0.378660 3.4:+9528763 None:intergenic
TCTGTGATGGAACATCTTAT+TGG 0.391840 3.4:-9528257 MS.gene046246:CDS
GGATATGCTAACAAATTTGA+TGG 0.406437 3.4:-9527568 MS.gene046246:CDS
CTCCCTCCTCCTTCTTGACC+TGG 0.408040 3.4:+9527529 None:intergenic
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3.4:-9528153 MS.gene046246:CDS
ACAATGGGTGGGACATGAAT+GGG 0.415171 3.4:+9528402 None:intergenic
TTCCATTTCTATCCCCTCTC+AGG 0.415536 3.4:-9528549 MS.gene046246:CDS
AACGACGGTAGGAGAGTTGC+TGG 0.419039 3.4:-9527809 MS.gene046246:CDS
GTGGATAGGTTGATGTGAAA+TGG 0.419118 3.4:+9528179 None:intergenic
TCAGAGAACAACTTGCAAAA+TGG 0.421540 3.4:-9527911 MS.gene046246:CDS
ATCTACACATTATAACCAAA+AGG 0.424756 3.4:-9528667 MS.gene046246:CDS
ATTTGGAATGGGAAAAGCTT+TGG 0.427618 3.4:-9527602 MS.gene046246:CDS
AATATGTCATTTAACACATT+GGG 0.429631 3.4:-9528703 MS.gene046246:CDS
ACATGCAGAAACGATGTTTA+GGG 0.431483 3.4:+9528764 None:intergenic
ATCAACCTATCCACAATAGA+TGG 0.431811 3.4:-9528170 MS.gene046246:CDS
ATTTGGTTTGAGTCTTCGAT+TGG 0.434962 3.4:+9528723 None:intergenic
TGTGAAATGGGACCATAAAC+AGG 0.441469 3.4:+9528192 None:intergenic
ATCACAGAGACAATGATTCA+TGG 0.442497 3.4:+9528272 None:intergenic
TTTGGTTTGAGTCTTCGATT+GGG 0.443458 3.4:+9528724 None:intergenic
TGCATGAATAAATTTAGCTC+CGG 0.444458 3.4:+9528458 None:intergenic
AATGGAAAAGCGTGAGGGAA+AGG 0.449937 3.4:+9528565 None:intergenic
TATGGAAATGAATTTGGAAT+GGG 0.460328 3.4:-9527613 MS.gene046246:CDS
ATGGAAAAGCGTGAGGGAAA+GGG 0.461186 3.4:+9528566 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.465501 3.4:+9528610 None:intergenic
TCAAAGAACGATTGGACAAT+GGG 0.466912 3.4:+9528387 None:intergenic
GAGGTGGTTCCATTCTTGTT+CGG 0.467336 3.4:+9527878 None:intergenic
CCAAAATATTCCTTAGGAAG+AGG 0.467793 3.4:+9527859 None:intergenic
CAATGGGTGGGACATGAATG+GGG 0.470273 3.4:+9528403 None:intergenic
ATGATAATAAAGTGGTGCTT+AGG 0.471109 3.4:-9527735 MS.gene046246:CDS
GACAATGGGTGGGACATGAA+TGG 0.472184 3.4:+9528401 None:intergenic
CAAGTCACAGAATTAGTAGA+TGG 0.474789 3.4:-9528312 MS.gene046246:CDS
GAATTCCCAAAATATTCCTT+AGG 0.477642 3.4:+9527853 None:intergenic
GGAAAAGTAATTTCATATCC+AGG 0.482689 3.4:-9527547 MS.gene046246:CDS
GAGTTGCTGGAGAATGATCT+AGG 0.486265 3.4:-9527796 MS.gene046246:CDS
TCACCGAGGTTTAATACGTA+TGG 0.489403 3.4:-9527631 MS.gene046246:CDS
AAATATGTCATTTAACACAT+TGG 0.493987 3.4:-9528704 MS.gene046246:CDS
AGTGTTTCAGTTTGTCCACC+AGG 0.507243 3.4:+9528589 None:intergenic
TGTTGGTTTAGTAATGATGT+TGG 0.510991 3.4:+9528627 None:intergenic
TGCAAATCATGATAATAAAG+TGG 0.522035 3.4:-9527743 MS.gene046246:CDS
TGCTGGAGAATGATCTAGGA+TGG 0.522825 3.4:-9527792 MS.gene046246:CDS
ACCTGAGAAAATGATGGTGC+TGG 0.523005 3.4:-9527482 MS.gene046246:CDS
TACAAACAACCGAACAAGAA+TGG 0.524034 3.4:-9527887 MS.gene046246:CDS
TGTCATGATCAAAGAACGAT+TGG 0.527909 3.4:+9528379 None:intergenic
GCATGAATAAATTTAGCTCC+GGG 0.529301 3.4:+9528459 None:intergenic
TGGATAGGTTGATGTGAAAT+GGG 0.531677 3.4:+9528180 None:intergenic
GTATGGAAATGAATTTGGAA+TGG 0.534748 3.4:-9527614 MS.gene046246:CDS
CCTTATACTGTGATGATAAG+TGG 0.535234 3.4:-9527655 MS.gene046246:CDS
AATGGAACCACCTCTTCCTA+AGG 0.542226 3.4:-9527869 MS.gene046246:CDS
TGTAGTGAGTGCTGAAACGA+CGG 0.546049 3.4:-9527824 MS.gene046246:CDS
TCTACACATTATAACCAAAA+GGG 0.546861 3.4:-9528666 MS.gene046246:CDS
GGAAACCATCTATTGTGGAT+AGG 0.548035 3.4:+9528165 None:intergenic
CGTGGTGATGTTTAAATGGA+AGG 0.548108 3.4:+9528118 None:intergenic
AGTAACAAGACGACCTGAGA+GGG 0.549751 3.4:+9528536 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3.4:+9528547 None:intergenic
AATGGGTGGGACATGAATGG+GGG 0.559109 3.4:+9528404 None:intergenic
AAAGCTTTGGCAGTTAGAAG+TGG 0.567341 3.4:-9527589 MS.gene046246:CDS
TTGGTTTGAGTCTTCGATTG+GGG 0.568932 3.4:+9528725 None:intergenic
ATGATCTAGGATGGGCAGCA+TGG 0.569534 3.4:-9527783 MS.gene046246:CDS
ATCAAAGAACGATTGGACAA+TGG 0.570569 3.4:+9528386 None:intergenic
ACGAAAACAGTATAAGAATG+AGG 0.571532 3.4:+9528498 None:intergenic
ATGAATCATTGTCTCTGTGA+TGG 0.575359 3.4:-9528270 MS.gene046246:CDS
TGCTTAGGTCGGTTGAAGAG+TGG 0.577268 3.4:-9527720 MS.gene046246:CDS
TATAGCGAAACTTAAAGCAA+AGG 0.578915 3.4:-9528031 MS.gene046246:CDS
TATCCAGGTCAAGAAGGAGG+AGG 0.584517 3.4:-9527532 MS.gene046246:CDS
CACAATAGATGGTTTCCAGA+AGG 0.584706 3.4:-9528159 MS.gene046246:CDS
ATTTCATATCCAGGTCAAGA+AGG 0.586316 3.4:-9527538 MS.gene046246:CDS
CAGAAACGATGTTTAGGGAA+GGG 0.587217 3.4:+9528769 None:intergenic
GTTATAATGTGTAGATAACA+TGG 0.589863 3.4:+9528674 None:intergenic
GACAAAGCAATCAACCATGA+TGG 0.590737 3.4:-9528360 MS.gene046246:CDS
ATAGTCAAAGATTATAACAA+AGG 0.594150 3.4:+9527418 None:intergenic
AAATATTCCTTAGGAAGAGG+TGG 0.594958 3.4:+9527862 None:intergenic
CTTCTGGAAACCATCTATTG+TGG 0.595445 3.4:+9528160 None:intergenic
ATCCAGGTCAAGAAGGAGGA+GGG 0.602246 3.4:-9527531 MS.gene046246:CDS
GCAGAAACGATGTTTAGGGA+AGG 0.605789 3.4:+9528768 None:intergenic
TTTCCATACGTATTAAACCT+CGG 0.609189 3.4:+9527628 None:intergenic
ACAACATTTCATAGAGAACC+TGG 0.610615 3.4:-9528607 MS.gene046246:CDS
AAGAACGATTGGACAATGGG+TGG 0.614907 3.4:+9528390 None:intergenic
AGAACGATTGGACAATGGGT+GGG 0.617288 3.4:+9528391 None:intergenic
TAATAAAGTGGTGCTTAGGT+CGG 0.624945 3.4:-9527731 MS.gene046246:CDS
GCTGGAGAATGATCTAGGAT+GGG 0.629384 3.4:-9527791 MS.gene046246:CDS
TGGTTTGAGTCTTCGATTGG+GGG 0.638810 3.4:+9528726 None:intergenic
GATAGAAATGGAAAAGCGTG+AGG 0.641029 3.4:+9528559 None:intergenic
TCTTTCACCTGAGAAAATGA+TGG 0.654677 3.4:-9527488 MS.gene046246:CDS
TCATATCCAGGTCAAGAAGG+AGG 0.656740 3.4:-9527535 MS.gene046246:CDS
AAGACATGGTGTGACCATCA+TGG 0.667161 3.4:+9528346 None:intergenic
GGCAGCATGGAAAATTCATG+TGG 0.671300 3.4:-9527770 MS.gene046246:CDS
GTGAGTGCTGAAACGACGGT+AGG 0.677302 3.4:-9527820 MS.gene046246:CDS
ATAGAAATGGAAAAGCGTGA+GGG 0.687050 3.4:+9528560 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3.4:+9528535 None:intergenic
CATGCACGTCGACTACAACA+GGG 0.700954 3.4:-9527934 MS.gene046246:CDS
TTGAATAGATAACAAAGACA+TGG 0.705301 3.4:+9528332 None:intergenic
GTAACAAGACGACCTGAGAG+GGG 0.714877 3.4:+9528537 None:intergenic
ACATTTCATAGAGAACCTGG+TGG 0.735789 3.4:-9528604 MS.gene046246:CDS
TGATGATAAGTGGTTCACCG+AGG 0.741768 3.4:-9527645 MS.gene046246:CDS
CATATGTGTGAACAAACTCG+TGG 0.754058 3.4:+9528100 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTAATCGATACAAAAA+AGG + chr3.4:9528521-9528540 None:intergenic 20.0%
!! AATGTGTTAAATGACATATT+TGG + chr3.4:9527503-9527522 None:intergenic 20.0%
!!! AAATATGTCATTTAACACAT+TGG - chr3.4:9527502-9527521 MS.gene046246:CDS 20.0%
!!! AAGATTCTCTCTTTTAAAAT+GGG + chr3.4:9528137-9528156 None:intergenic 20.0%
!!! AATATGTCATTTAACACATT+GGG - chr3.4:9527503-9527522 MS.gene046246:CDS 20.0%
! AATACGTATGGAAATGAATT+TGG - chr3.4:9528587-9528606 MS.gene046246:CDS 25.0%
! AATCTTTATCAGCATTAGTT+TGG - chr3.4:9528237-9528256 MS.gene046246:CDS 25.0%
! ATCTACACATTATAACCAAA+AGG - chr3.4:9527539-9527558 MS.gene046246:CDS 25.0%
! CAAAATGATATTCCTGTTTA+TGG - chr3.4:9528002-9528021 MS.gene046246:CDS 25.0%
! GTTATAATGTGTAGATAACA+TGG + chr3.4:9527535-9527554 None:intergenic 25.0%
! TCTACACATTATAACCAAAA+GGG - chr3.4:9527540-9527559 MS.gene046246:CDS 25.0%
! TGCAAATCATGATAATAAAG+TGG - chr3.4:9528463-9528482 MS.gene046246:CDS 25.0%
! TTGAATAGATAACAAAGACA+TGG + chr3.4:9527877-9527896 None:intergenic 25.0%
!! TATGGAAATGAATTTGGAAT+GGG - chr3.4:9528593-9528612 MS.gene046246:CDS 25.0%
!!! GAAGATTCTCTCTTTTAAAA+TGG + chr3.4:9528138-9528157 None:intergenic 25.0%
!!! TTAAATGGAAGGTTGATAAT+TGG + chr3.4:9528080-9528099 None:intergenic 25.0%
ACGAAAACAGTATAAGAATG+AGG + chr3.4:9527711-9527730 None:intergenic 30.0%
ATGATAATAAAGTGGTGCTT+AGG - chr3.4:9528471-9528490 MS.gene046246:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + chr3.4:9528356-9528375 None:intergenic 30.0%
GGAAAAGTAATTTCATATCC+AGG - chr3.4:9528659-9528678 MS.gene046246:CDS 30.0%
GGATATGCTAACAAATTTGA+TGG - chr3.4:9528638-9528657 MS.gene046246:CDS 30.0%
GTATGGAAATGAATTTGGAA+TGG - chr3.4:9528592-9528611 MS.gene046246:CDS 30.0%
TATAGCGAAACTTAAAGCAA+AGG - chr3.4:9528175-9528194 MS.gene046246:CDS 30.0%
TTTCCATACGTATTAAACCT+CGG + chr3.4:9528581-9528600 None:intergenic 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - chr3.4:9528348-9528367 MS.gene046246:CDS 30.0%
! TGCATGAATAAATTTAGCTC+CGG + chr3.4:9527751-9527770 None:intergenic 30.0%
! TTAGTAGATGGTGTTTTCAT+TGG - chr3.4:9527906-9527925 MS.gene046246:CDS 30.0%
!! TGTTGGTTTAGTAATGATGT+TGG + chr3.4:9527582-9527601 None:intergenic 30.0%
AAATATTCCTTAGGAAGAGG+TGG + chr3.4:9528347-9528366 None:intergenic 35.0%
AACATGCAGAAACGATGTTT+AGG + chr3.4:9527446-9527465 None:intergenic 35.0%
ACAACATTTCATAGAGAACC+TGG - chr3.4:9527599-9527618 MS.gene046246:CDS 35.0%
ACATGCAGAAACGATGTTTA+GGG + chr3.4:9527445-9527464 None:intergenic 35.0%
ATAATTGGACCATAACCTTC+TGG + chr3.4:9528065-9528084 None:intergenic 35.0%
ATCAAAGAACGATTGGACAA+TGG + chr3.4:9527823-9527842 None:intergenic 35.0%
ATCAACCTATCCACAATAGA+TGG - chr3.4:9528036-9528055 MS.gene046246:CDS 35.0%
ATCACAGAGACAATGATTCA+TGG + chr3.4:9527937-9527956 None:intergenic 35.0%
ATGAATCATTGTCTCTGTGA+TGG - chr3.4:9527936-9527955 MS.gene046246:CDS 35.0%
ATTTCATATCCAGGTCAAGA+AGG - chr3.4:9528668-9528687 MS.gene046246:CDS 35.0%
CAAGTCACAGAATTAGTAGA+TGG - chr3.4:9527894-9527913 MS.gene046246:CDS 35.0%
CCAAAATATTCCTTAGGAAG+AGG + chr3.4:9528350-9528369 None:intergenic 35.0%
CCACTTATCATCACAGTATA+AGG + chr3.4:9528554-9528573 None:intergenic 35.0%
CCTTATACTGTGATGATAAG+TGG - chr3.4:9528551-9528570 MS.gene046246:CDS 35.0%
CTATAGATTCCGCTGAAAAA+TGG + chr3.4:9528160-9528179 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + chr3.4:9527599-9527618 None:intergenic 35.0%
TAATAAAGTGGTGCTTAGGT+CGG - chr3.4:9528475-9528494 MS.gene046246:CDS 35.0%
TACAAACAACCGAACAAGAA+TGG - chr3.4:9528319-9528338 MS.gene046246:CDS 35.0%
TCAAAGAACGATTGGACAAT+GGG + chr3.4:9527822-9527841 None:intergenic 35.0%
TCAGAGAACAACTTGCAAAA+TGG - chr3.4:9528295-9528314 MS.gene046246:CDS 35.0%
TCTTTCACCTGAGAAAATGA+TGG - chr3.4:9528718-9528737 MS.gene046246:CDS 35.0%
TGTCATGATCAAAGAACGAT+TGG + chr3.4:9527830-9527849 None:intergenic 35.0%
! AACTCGTGGTGATGTTTAAA+TGG + chr3.4:9528095-9528114 None:intergenic 35.0%
! ATAGAAATGGAAAAGCGTGA+GGG + chr3.4:9527649-9527668 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCTT+TGG - chr3.4:9528604-9528623 MS.gene046246:CDS 35.0%
! CCTCTTCCTAAGGAATATTT+TGG - chr3.4:9528347-9528366 MS.gene046246:CDS 35.0%
! GCATGAATAAATTTAGCTCC+GGG + chr3.4:9527750-9527769 None:intergenic 35.0%
! TCTGTGATGGAACATCTTAT+TGG - chr3.4:9527949-9527968 MS.gene046246:CDS 35.0%
!! ATTTGGTTTGAGTCTTCGAT+TGG + chr3.4:9527486-9527505 None:intergenic 35.0%
!! GAGAATCTTCCATTTTTCAG+CGG - chr3.4:9528148-9528167 MS.gene046246:CDS 35.0%
!! TGGATAGGTTGATGTGAAAT+GGG + chr3.4:9528029-9528048 None:intergenic 35.0%
!! TTTGGTTTGAGTCTTCGATT+GGG + chr3.4:9527485-9527504 None:intergenic 35.0%
!! TTTTGAAGAGAAGTCCCTTT+TGG + chr3.4:9527557-9527576 None:intergenic 35.0%
ACATTTCATAGAGAACCTGG+TGG - chr3.4:9527602-9527621 MS.gene046246:CDS 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - chr3.4:9528053-9528072 MS.gene046246:CDS 40.0%
CACAATAGATGGTTTCCAGA+AGG - chr3.4:9528047-9528066 MS.gene046246:CDS 40.0%
CAGAAACGATGTTTAGGGAA+GGG + chr3.4:9527440-9527459 None:intergenic 40.0%
CATATGTGTGAACAAACTCG+TGG + chr3.4:9528109-9528128 None:intergenic 40.0%
GACAAAGCAATCAACCATGA+TGG - chr3.4:9527846-9527865 MS.gene046246:CDS 40.0%
GGAAACCATCTATTGTGGAT+AGG + chr3.4:9528044-9528063 None:intergenic 40.0%
TCACCGAGGTTTAATACGTA+TGG - chr3.4:9528575-9528594 MS.gene046246:CDS 40.0%
TGTGAAATGGGACCATAAAC+AGG + chr3.4:9528017-9528036 None:intergenic 40.0%
! AAAGCTTTGGCAGTTAGAAG+TGG - chr3.4:9528617-9528636 MS.gene046246:CDS 40.0%
! CACGAGTTTGTTCACACATA+TGG - chr3.4:9528107-9528126 MS.gene046246:CDS 40.0%
! CGTGGTGATGTTTAAATGGA+AGG + chr3.4:9528091-9528110 None:intergenic 40.0%
! CTTCTGGAAACCATCTATTG+TGG + chr3.4:9528049-9528068 None:intergenic 40.0%
! GATAGAAATGGAAAAGCGTG+AGG + chr3.4:9527650-9527669 None:intergenic 40.0%
! TTGGTTTGAGTCTTCGATTG+GGG + chr3.4:9527484-9527503 None:intergenic 40.0%
!! GTGGATAGGTTGATGTGAAA+TGG + chr3.4:9528030-9528049 None:intergenic 40.0%
AAGAACGATTGGACAATGGG+TGG + chr3.4:9527819-9527838 None:intergenic 45.0%
AAGACATGGTGTGACCATCA+TGG + chr3.4:9527863-9527882 None:intergenic 45.0%
AATGGAACCACCTCTTCCTA+AGG - chr3.4:9528337-9528356 MS.gene046246:CDS 45.0%
ACAATGGGTGGGACATGAAT+GGG + chr3.4:9527807-9527826 None:intergenic 45.0%
AGAACGATTGGACAATGGGT+GGG + chr3.4:9527818-9527837 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + chr3.4:9527673-9527692 None:intergenic 45.0%
AGTGTTTCAGTTTGTCCACC+AGG + chr3.4:9527620-9527639 None:intergenic 45.0%
GAGGTGGTTCCATTCTTGTT+CGG + chr3.4:9528331-9528350 None:intergenic 45.0%
GAGTTGCTGGAGAATGATCT+AGG - chr3.4:9528410-9528429 MS.gene046246:CDS 45.0%
GCAGAAACGATGTTTAGGGA+AGG + chr3.4:9527441-9527460 None:intergenic 45.0%
GGCAGCATGGAAAATTCATG+TGG - chr3.4:9528436-9528455 MS.gene046246:CDS 45.0%
GGGGGTGAGGATATCATTTA+TGG + chr3.4:9527787-9527806 None:intergenic 45.0%
GTCGATTGCAAAAACAGTCC+CGG - chr3.4:9527729-9527748 MS.gene046246:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + chr3.4:9527674-9527693 None:intergenic 45.0%
TCATATCCAGGTCAAGAAGG+AGG - chr3.4:9528671-9528690 MS.gene046246:CDS 45.0%
TGATGATAAGTGGTTCACCG+AGG - chr3.4:9528561-9528580 MS.gene046246:CDS 45.0%
TGCTGGAGAATGATCTAGGA+TGG - chr3.4:9528414-9528433 MS.gene046246:CDS 45.0%
TGGTTTGAGTCTTCGATTGG+GGG + chr3.4:9527483-9527502 None:intergenic 45.0%
TTCCATTTCTATCCCCTCTC+AGG - chr3.4:9527657-9527676 MS.gene046246:CDS 45.0%
! AATGGAAAAGCGTGAGGGAA+AGG + chr3.4:9527644-9527663 None:intergenic 45.0%
! ACCTGAGAAAATGATGGTGC+TGG - chr3.4:9528724-9528743 MS.gene046246:CDS 45.0%
! ATGGAAAAGCGTGAGGGAAA+GGG + chr3.4:9527643-9527662 None:intergenic 45.0%
! GCTGGAGAATGATCTAGGAT+GGG - chr3.4:9528415-9528434 MS.gene046246:CDS 45.0%
! TCCAGCACCATCATTTTCTC+AGG + chr3.4:9528728-9528747 None:intergenic 45.0%
!! TGTAGTGAGTGCTGAAACGA+CGG - chr3.4:9528382-9528401 MS.gene046246:CDS 45.0%
AATGGGTGGGACATGAATGG+GGG + chr3.4:9527805-9527824 None:intergenic 50.0%
ATCCAGGTCAAGAAGGAGGA+GGG - chr3.4:9528675-9528694 MS.gene046246:CDS 50.0%
CAATGGGTGGGACATGAATG+GGG + chr3.4:9527806-9527825 None:intergenic 50.0%
GACAATGGGTGGGACATGAA+TGG + chr3.4:9527808-9527827 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + chr3.4:9527662-9527681 None:intergenic 50.0%
GAGTCTTCGATTGGGGGATA+TGG + chr3.4:9527477-9527496 None:intergenic 50.0%
GTAACAAGACGACCTGAGAG+GGG + chr3.4:9527672-9527691 None:intergenic 50.0%
TATCCAGGTCAAGAAGGAGG+AGG - chr3.4:9528674-9528693 MS.gene046246:CDS 50.0%
TGCTTAGGTCGGTTGAAGAG+TGG - chr3.4:9528486-9528505 MS.gene046246:CDS 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - chr3.4:9528423-9528442 MS.gene046246:CDS 50.0%
! CATGCACGTCGACTACAACA+GGG - chr3.4:9528272-9528291 MS.gene046246:CDS 50.0%
! TCATGCACGTCGACTACAAC+AGG - chr3.4:9528271-9528290 MS.gene046246:CDS 50.0%
AACGACGGTAGGAGAGTTGC+TGG - chr3.4:9528397-9528416 MS.gene046246:CDS 55.0%
!! GTGAGTGCTGAAACGACGGT+AGG - chr3.4:9528386-9528405 MS.gene046246:CDS 55.0%
CTCCCTCCTCCTTCTTGACC+TGG + chr3.4:9528680-9528699 None:intergenic 60.0%
GTGGGACATGAATGGGGGTG+AGG + chr3.4:9527800-9527819 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 9527429 9528799 9527429 ID=MS.gene046246
chr3.4 mRNA 9527429 9528799 9527429 ID=MS.gene046246.t1;Parent=MS.gene046246
chr3.4 exon 9527429 9528799 9527429 ID=MS.gene046246.t1.exon1;Parent=MS.gene046246.t1
chr3.4 CDS 9527429 9528799 9527429 ID=cds.MS.gene046246.t1;Parent=MS.gene046246.t1
Gene Sequence

>MS.gene046246

ATGAGCATCCCTTCCCTAAACATCGTTTCTGCATGTTTCATTAAACCATATCCCCCAATCGAAGACTCAAACCAAATATGTCATTTAACACATTGGGATATTGCCATGTTATCTACACATTATAACCAAAAGGGACTTCTCTTCAAAAAACCAACATCATTACTAAACCAACAACATTTCATAGAGAACCTGGTGGACAAACTGAAACACTCCCTTTCCCTCACGCTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCTGAAGATCCTCATTCTTATACTGTTTTCGTCGATTGCAAAAACAGTCCCGGAGCTAAATTTATTCATGCAACTTTAGATATCACCATAAATGATATCCTCACCCCCATTCATGTCCCACCCATTGTCCAATCGTTCTTTGATCATGACAAAGCAATCAACCATGATGGTCACACCATGTCTTTGTTATCTATTCAAGTCACAGAATTAGTAGATGGTGTTTTCATTGGTTGTTCCATGAATCATTGTCTCTGTGATGGAACATCTTATTGGAATTTCTTCAACACATTTTCTGAGATATTTCAAAATGATATTCCTGTTTATGGTCCCATTTCACATCAACCTATCCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATTATCAACCTTCCATTTAAACATCACCACGAGTTTGTTCACACATATGGAATACCCATTTTAAAAGAGAGAATCTTCCATTTTTCAGCGGAATCTATAGCGAAACTTAAAGCAAAGGCAAATGCAGAGTCTAATACAAACAAAATTTCTTCGTTTCAATCTTTATCAGCATTAGTTTGGAGATCTATAACTCATGCACGTCGACTACAACAGGGTCAGAGAACAACTTGCAAAATGGCTACAAACAACCGAACAAGAATGGAACCACCTCTTCCTAAGGAATATTTTGGGAATTCAATTTATGTAGTGAGTGCTGAAACGACGGTAGGAGAGTTGCTGGAGAATGATCTAGGATGGGCAGCATGGAAAATTCATGTGGCTATTGCAAATCATGATAATAAAGTGGTGCTTAGGTCGGTTGAAGAGTGGTTTCGTTCTCCTTTTTTGTATCGATTAGATTTGTTTTTTGATCCTTATACTGTGATGATAAGTGGTTCACCGAGGTTTAATACGTATGGAAATGAATTTGGAATGGGAAAAGCTTTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAATTTCATATCCAGGTCAAGAAGGAGGAGGGAGCATAGATCTTGAAGTGTGTCTTTCACCTGAGAAAATGATGGTGCTGGAGACAGATGAAGAGTTCATGAACTCAGTTTCTGTGTTTAATCCTTTGTTATAA
Protein sequence

>MS.gene046246.t1

MSIPSLNIVSACFIKPYPPIEDSNQICHLTHWDIAMLSTHYNQKGLLFKKPTSLLNQQHFIENLVDKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCKNSPGAKFIHATLDITINDILTPIHVPPIVQSFFDHDKAINHDGHTMSLLSIQVTELVDGVFIGCSMNHCLCDGTSYWNFFNTFSEIFQNDIPVYGPISHQPIHNRWFPEGYGPIINLPFKHHHEFVHTYGIPILKERIFHFSAESIAKLKAKANAESNTNKISSFQSLSALVWRSITHARRLQQGQRTTCKMATNNRTRMEPPLPKEYFGNSIYVVSAETTVGELLENDLGWAAWKIHVAIANHDNKVVLRSVEEWFRSPFLYRLDLFFDPYTVMISGSPRFNTYGNEFGMGKALAVRSGYANKFDGKVISYPGQEGGGSIDLEVCLSPEKMMVLETDEEFMNSVSVFNPLL