Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22296.t1 | XP_003610974.1 | 99.5 | 219 | 1 | 0 | 1 | 219 | 180 | 398 | 2.90E-118 | 434.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22296.t1 | Q43078 | 94.5 | 219 | 12 | 0 | 1 | 219 | 191 | 409 | 2.0e-115 | 416.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22296.t1 | A0A072UDA7 | 99.5 | 219 | 1 | 0 | 1 | 219 | 32 | 250 | 2.1e-118 | 434.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene22296 | 0.806447 | 8.19E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene22296 | 0.801062 | 1.07E-48 | -1.69E-46 |
| MS.gene04933 | MS.gene22296 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene049351 | MS.gene22296 | 0.873793 | 1.12E-67 | -1.69E-46 |
| MS.gene049661 | MS.gene22296 | 0.818545 | 1.85E-52 | -1.69E-46 |
| MS.gene049662 | MS.gene22296 | 0.81304 | 3.13E-51 | -1.69E-46 |
| MS.gene049681 | MS.gene22296 | 0.848224 | 7.02E-60 | -1.69E-46 |
| MS.gene049758 | MS.gene22296 | 0.82517 | 5.42E-54 | -1.69E-46 |
| MS.gene049993 | MS.gene22296 | 0.816015 | 6.88E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene22296 | 0.803929 | 2.76E-49 | -1.69E-46 |
| MS.gene050231 | MS.gene22296 | 0.815086 | 1.11E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene22296 | 0.878853 | 2.01E-69 | -1.69E-46 |
| MS.gene050397 | MS.gene22296 | 0.826384 | 2.79E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene22296 | 0.814917 | 1.21E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene22296 | 0.822421 | 2.39E-53 | -1.69E-46 |
| MS.gene050560 | MS.gene22296 | 0.807483 | 4.94E-50 | -1.69E-46 |
| MS.gene050650 | MS.gene22296 | 0.813653 | 2.30E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene22296 | 0.878829 | 2.05E-69 | -1.69E-46 |
| MS.gene050783 | MS.gene22296 | 0.806757 | 7.04E-50 | -1.69E-46 |
| MS.gene050787 | MS.gene22296 | 0.822617 | 2.15E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene22296 | 0.844627 | 6.72E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene22296 | 0.823536 | 1.31E-53 | -1.69E-46 |
| MS.gene051360 | MS.gene22296 | 0.802016 | 6.85E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene22296 | 0.837693 | 4.47E-57 | -1.69E-46 |
| MS.gene051525 | MS.gene22296 | 0.844422 | 7.64E-59 | -1.69E-46 |
| MS.gene051582 | MS.gene22296 | 0.856624 | 2.84E-62 | -1.69E-46 |
| MS.gene051860 | MS.gene22296 | 0.857601 | 1.46E-62 | -1.69E-46 |
| MS.gene051877 | MS.gene22296 | 0.837318 | 5.58E-57 | -1.69E-46 |
| MS.gene052011 | MS.gene22296 | -0.863907 | 1.78E-64 | -1.69E-46 |
| MS.gene052078 | MS.gene22296 | 0.822776 | 1.98E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene22296 | 0.853685 | 2.03E-61 | -1.69E-46 |
| MS.gene052207 | MS.gene22296 | 0.845336 | 4.33E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene22296 | 0.87804 | 3.87E-69 | -1.69E-46 |
| MS.gene052310 | MS.gene22296 | 0.828788 | 7.40E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene22296 | 0.814838 | 1.26E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene22296 | 0.805662 | 1.20E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene22296 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene052421 | MS.gene22296 | 0.807917 | 4.00E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene22296 | 0.808105 | 3.65E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene22296 | 0.847257 | 1.30E-59 | -1.69E-46 |
| MS.gene052767 | MS.gene22296 | 0.819668 | 1.03E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene22296 | 0.802118 | 6.53E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene22296 | 0.800126 | 1.67E-48 | -1.69E-46 |
| MS.gene053209 | MS.gene22296 | 0.807163 | 5.78E-50 | -1.69E-46 |
| MS.gene053758 | MS.gene22296 | 0.8004 | 1.47E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene22296 | 0.878297 | 3.15E-69 | -1.69E-46 |
| MS.gene053928 | MS.gene22296 | 0.806516 | 7.92E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene22296 | 0.831613 | 1.51E-55 | -1.69E-46 |
| MS.gene054011 | MS.gene22296 | 0.852323 | 4.98E-61 | -1.69E-46 |
| MS.gene054012 | MS.gene22296 | 0.832357 | 9.90E-56 | -1.69E-46 |
| MS.gene054013 | MS.gene22296 | 0.826087 | 3.29E-54 | -1.69E-46 |
| MS.gene054123 | MS.gene22296 | 0.841396 | 4.87E-58 | -1.69E-46 |
| MS.gene054183 | MS.gene22296 | 0.872605 | 2.80E-67 | -1.69E-46 |
| MS.gene054473 | MS.gene22296 | 0.859981 | 2.84E-63 | -1.69E-46 |
| MS.gene054556 | MS.gene22296 | 0.822881 | 1.87E-53 | -1.69E-46 |
| MS.gene05455 | MS.gene22296 | 0.819118 | 1.37E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene22296 | 0.818991 | 1.47E-52 | -1.69E-46 |
| MS.gene054917 | MS.gene22296 | 0.840079 | 1.08E-57 | -1.69E-46 |
| MS.gene054918 | MS.gene22296 | 0.828917 | 6.89E-55 | -1.69E-46 |
| MS.gene054920 | MS.gene22296 | 0.814601 | 1.42E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene22296 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene22296 | 0.854391 | 1.27E-61 | -1.69E-46 |
| MS.gene055277 | MS.gene22296 | 0.846237 | 2.46E-59 | -1.69E-46 |
| MS.gene055369 | MS.gene22296 | 0.81538 | 9.52E-52 | -1.69E-46 |
| MS.gene055390 | MS.gene22296 | 0.840685 | 7.49E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene22296 | 0.864781 | 9.50E-65 | -1.69E-46 |
| MS.gene055428 | MS.gene22296 | 0.849975 | 2.29E-60 | -1.69E-46 |
| MS.gene05553 | MS.gene22296 | 0.819736 | 9.93E-53 | -1.69E-46 |
| MS.gene055564 | MS.gene22296 | 0.845266 | 4.52E-59 | -1.69E-46 |
| MS.gene055604 | MS.gene22296 | 0.853802 | 1.88E-61 | -1.69E-46 |
| MS.gene05594 | MS.gene22296 | 0.865103 | 7.53E-65 | -1.69E-46 |
| MS.gene05602 | MS.gene22296 | 0.823799 | 1.14E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene22296 | 0.840221 | 9.91E-58 | -1.69E-46 |
| MS.gene056337 | MS.gene22296 | 0.822384 | 2.44E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene22296 | 0.880379 | 5.76E-70 | -1.69E-46 |
| MS.gene056410 | MS.gene22296 | 0.803303 | 3.72E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene22296 | 0.801612 | 8.29E-49 | -1.69E-46 |
| MS.gene056514 | MS.gene22296 | 0.81071 | 1.01E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene22296 | 0.818504 | 1.89E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene22296 | 0.834359 | 3.14E-56 | -1.69E-46 |
| MS.gene056723 | MS.gene22296 | 0.818626 | 1.78E-52 | -1.69E-46 |
| MS.gene056846 | MS.gene22296 | 0.821206 | 4.57E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene22296 | -0.808008 | 3.82E-50 | -1.69E-46 |
| MS.gene056902 | MS.gene22296 | 0.824904 | 6.27E-54 | -1.69E-46 |
| MS.gene056931 | MS.gene22296 | 0.811132 | 8.16E-51 | -1.69E-46 |
| MS.gene056933 | MS.gene22296 | 0.805451 | 1.33E-49 | -1.69E-46 |
| MS.gene056935 | MS.gene22296 | 0.802474 | 5.51E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene22296 | 0.840697 | 7.44E-58 | -1.69E-46 |
| MS.gene057121 | MS.gene22296 | 0.852694 | 3.90E-61 | -1.69E-46 |
| MS.gene057124 | MS.gene22296 | 0.813181 | 2.92E-51 | -1.69E-46 |
| MS.gene057146 | MS.gene22296 | 0.835545 | 1.58E-56 | -1.69E-46 |
| MS.gene057245 | MS.gene22296 | 0.862591 | 4.55E-64 | -1.69E-46 |
| MS.gene057439 | MS.gene22296 | 0.807907 | 4.02E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene22296 | 0.815795 | 7.70E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene22296 | 0.802476 | 5.51E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene22296 | 0.814635 | 1.39E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene22296 | 0.860997 | 1.40E-63 | -1.69E-46 |
| MS.gene057476 | MS.gene22296 | 0.857904 | 1.19E-62 | -1.69E-46 |
| MS.gene057749 | MS.gene22296 | 0.85402 | 1.63E-61 | -1.69E-46 |
| MS.gene057823 | MS.gene22296 | 0.853301 | 2.62E-61 | -1.69E-46 |
| MS.gene057825 | MS.gene22296 | 0.812557 | 3.99E-51 | -1.69E-46 |
| MS.gene057826 | MS.gene22296 | 0.802089 | 6.62E-49 | -1.69E-46 |
| MS.gene057888 | MS.gene22296 | 0.846067 | 2.74E-59 | -1.69E-46 |
| MS.gene058002 | MS.gene22296 | 0.863254 | 2.84E-64 | -1.69E-46 |
| MS.gene058007 | MS.gene22296 | 0.807901 | 4.03E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene22296 | 0.848106 | 7.57E-60 | -1.69E-46 |
| MS.gene058142 | MS.gene22296 | 0.836944 | 6.95E-57 | -1.69E-46 |
| MS.gene058293 | MS.gene22296 | 0.809375 | 1.95E-50 | -1.69E-46 |
| MS.gene05842 | MS.gene22296 | 0.810333 | 1.21E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene22296 | 0.876348 | 1.50E-68 | -1.69E-46 |
| MS.gene058700 | MS.gene22296 | 0.832886 | 7.32E-56 | -1.69E-46 |
| MS.gene058710 | MS.gene22296 | 0.819508 | 1.12E-52 | -1.69E-46 |
| MS.gene058739 | MS.gene22296 | 0.806805 | 6.88E-50 | -1.69E-46 |
| MS.gene05886 | MS.gene22296 | 0.9179 | 3.02E-86 | -1.69E-46 |
| MS.gene058928 | MS.gene22296 | 0.804248 | 2.37E-49 | -1.69E-46 |
| MS.gene058949 | MS.gene22296 | 0.829069 | 6.33E-55 | -1.69E-46 |
| MS.gene058993 | MS.gene22296 | 0.809663 | 1.69E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene22296 | 0.879295 | 1.40E-69 | -1.69E-46 |
| MS.gene059082 | MS.gene22296 | 0.822825 | 1.93E-53 | -1.69E-46 |
| MS.gene059141 | MS.gene22296 | 0.817725 | 2.84E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene22296 | 0.804744 | 1.87E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene22296 | 0.87389 | 1.03E-67 | -1.69E-46 |
| MS.gene059462 | MS.gene22296 | 0.842214 | 2.96E-58 | -1.69E-46 |
| MS.gene059518 | MS.gene22296 | 0.811126 | 8.18E-51 | -1.69E-46 |
| MS.gene059597 | MS.gene22296 | 0.828578 | 8.32E-55 | -1.69E-46 |
| MS.gene059676 | MS.gene22296 | 0.831604 | 1.52E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene22296 | 0.860772 | 1.64E-63 | -1.69E-46 |
| MS.gene059692 | MS.gene22296 | 0.836181 | 1.09E-56 | -1.69E-46 |
| MS.gene059695 | MS.gene22296 | 0.837474 | 5.09E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene22296 | 0.843614 | 1.26E-58 | -1.69E-46 |
| MS.gene060038 | MS.gene22296 | 0.804241 | 2.37E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene22296 | 0.830028 | 3.70E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene22296 | 0.840353 | 9.15E-58 | -1.69E-46 |
| MS.gene060559 | MS.gene22296 | 0.818947 | 1.50E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene22296 | 0.811666 | 6.25E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene22296 | 0.84493 | 5.57E-59 | -1.69E-46 |
| MS.gene061051 | MS.gene22296 | -0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene22296 | 0.82057 | 6.40E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22296.t1 | MTR_5g009110 | 99.543 | 219 | 1 | 0 | 1 | 219 | 32 | 250 | 5.65e-161 | 447 |
| MS.gene22296.t1 | MTR_5g009110 | 99.543 | 219 | 1 | 0 | 1 | 219 | 180 | 398 | 8.92e-160 | 450 |
| MS.gene22296.t1 | MTR_5g009110 | 99.543 | 219 | 1 | 0 | 1 | 219 | 32 | 250 | 1.48e-158 | 445 |
| MS.gene22296.t1 | MTR_5g009110 | 99.543 | 219 | 1 | 0 | 1 | 219 | 180 | 398 | 1.73e-157 | 448 |
| MS.gene22296.t1 | MTR_7g079440 | 47.964 | 221 | 101 | 5 | 1 | 219 | 295 | 503 | 1.54e-53 | 181 |
| MS.gene22296.t1 | MTR_7g079440 | 47.964 | 221 | 101 | 5 | 1 | 219 | 295 | 503 | 5.51e-53 | 181 |
| MS.gene22296.t1 | MTR_1g062190 | 44.700 | 217 | 111 | 4 | 3 | 219 | 187 | 394 | 1.95e-51 | 174 |
| MS.gene22296.t1 | MTR_7g079440 | 46.569 | 204 | 95 | 5 | 1 | 202 | 295 | 486 | 7.66e-48 | 164 |
| MS.gene22296.t1 | MTR_8g088470 | 26.178 | 191 | 118 | 6 | 25 | 208 | 155 | 329 | 8.14e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22296.t1 | AT4G15110 | 80.909 | 220 | 41 | 1 | 1 | 219 | 187 | 406 | 2.59e-128 | 374 |
| MS.gene22296.t1 | AT1G31800 | 49.545 | 220 | 99 | 4 | 1 | 219 | 216 | 424 | 1.26e-53 | 181 |
| MS.gene22296.t1 | AT3G53130 | 45.161 | 217 | 110 | 4 | 3 | 219 | 187 | 394 | 2.00e-51 | 174 |
Find 57 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAATCTCCCGTTATTA+AGG | 0.215007 | 5.4:-3836790 | MS.gene22296:intron |
| AATTTGGCGCTTGATATTAT+TGG | 0.220263 | 5.4:-3836849 | MS.gene22296:CDS |
| GCTAATGGAAATTTCCAATA+TGG | 0.273639 | 5.4:+3836329 | None:intergenic |
| CCACTTTCTATATTCCATAT+TGG | 0.290956 | 5.4:-3836343 | MS.gene22296:CDS |
| ATTTGGCGCTTGATATTATT+GGG | 0.305440 | 5.4:-3836848 | MS.gene22296:CDS |
| ATCCTGAAACTTCCTTTGCC+TGG | 0.324503 | 5.4:+3836292 | None:intergenic |
| CTTAATAACGGGAGATTCAT+TGG | 0.337741 | 5.4:+3836791 | None:intergenic |
| ACCCAGGCAAAGGAAGTTTC+AGG | 0.341371 | 5.4:-3836294 | MS.gene22296:CDS |
| GGTACTATCCACCTTGCTAA+TGG | 0.381578 | 5.4:+3836314 | None:intergenic |
| GTAGATTCGGTGTTGGGTAC+GGG | 0.410444 | 5.4:-3835436 | MS.gene22296:CDS |
| TAATACTGTTCTTTCTGAAC+AGG | 0.417668 | 5.4:+3836950 | None:intergenic |
| GCGCTTGATATTATTGGGCT+AGG | 0.417860 | 5.4:-3836843 | MS.gene22296:CDS |
| GGTGTGTTCAACTATGACTT+TGG | 0.418560 | 5.4:-3836822 | MS.gene22296:CDS |
| CGACGGCTGCAGTTCTTACA+TGG | 0.418776 | 5.4:-3835644 | MS.gene22296:CDS |
| TTTAGTCAAACCTTAATAAC+GGG | 0.423879 | 5.4:+3836780 | None:intergenic |
| GACAAGTATTAATGACCTTA+AGG | 0.428207 | 5.4:+3836267 | None:intergenic |
| TTAATGACAATGCTTATTGC+TGG | 0.433494 | 5.4:-3835675 | MS.gene22296:CDS |
| GGCTCAAGCAGAGGTAGATT+CGG | 0.433948 | 5.4:-3835449 | MS.gene22296:CDS |
| CTTGAAGGAGAGGGGTATAG+TGG | 0.436234 | 5.4:-3836912 | MS.gene22296:CDS |
| AAGATCGATAAGCTTCTTGA+AGG | 0.436267 | 5.4:-3836927 | MS.gene22296:CDS |
| TCCTGAAACTTCCTTTGCCT+GGG | 0.437516 | 5.4:+3836293 | None:intergenic |
| TGGGCAGAAGTCAATCGAAT+TGG | 0.439863 | 5.4:-3836892 | MS.gene22296:CDS |
| GCAGAGGTAGATTCGGTGTT+GGG | 0.454361 | 5.4:-3835442 | MS.gene22296:CDS |
| GGTAGATTCGGTGTTGGGTA+CGG | 0.455717 | 5.4:-3835437 | MS.gene22296:CDS |
| GAAGTTTCAGGATGACCTTA+AGG | 0.459363 | 5.4:-3836282 | MS.gene22296:CDS |
| CTTTAGTCAAACCTTAATAA+CGG | 0.469753 | 5.4:+3836779 | None:intergenic |
| CAGAAATGCAAAAGAGAGTA+GGG | 0.471868 | 5.4:-3836231 | MS.gene22296:CDS |
| AGTTGATGTTGATGATCGTC+AGG | 0.498635 | 5.4:-3835872 | MS.gene22296:intron |
| AAGGGATTACTCAAATTTGA+AGG | 0.502615 | 5.4:-3836056 | MS.gene22296:intron |
| TCAGAAATGCAAAAGAGAGT+AGG | 0.502882 | 5.4:-3836232 | MS.gene22296:CDS |
| GTCATTAATACTTGTCTCGA+TGG | 0.505907 | 5.4:-3836260 | MS.gene22296:CDS |
| CCAATATGGAATATAGAAAG+TGG | 0.514457 | 5.4:+3836343 | None:intergenic |
| GCGCTTTCTAGTTGATATGC+GGG | 0.518450 | 5.4:-3835896 | MS.gene22296:CDS |
| ATGTTGAGAAACTGCAGCAA+AGG | 0.522240 | 5.4:-3836075 | MS.gene22296:CDS |
| AATCTGTTACAGGCAGTGTA+TGG | 0.523243 | 5.4:-3836392 | MS.gene22296:intron |
| ATTAACATACTTGAGCTAGC+AGG | 0.528163 | 5.4:+3835613 | None:intergenic |
| TGCGCTTTCTAGTTGATATG+CGG | 0.528290 | 5.4:-3835897 | MS.gene22296:CDS |
| ATTGGAAATTTCCATTAGCA+AGG | 0.528710 | 5.4:-3836325 | MS.gene22296:CDS |
| TGTTGAGAAACTGCAGCAAA+GGG | 0.541358 | 5.4:-3836074 | MS.gene22296:CDS |
| TAGATTCGGTGTTGGGTACG+GGG | 0.552238 | 5.4:-3835435 | MS.gene22296:CDS |
| AGCAGAGGTAGATTCGGTGT+TGG | 0.555564 | 5.4:-3835443 | MS.gene22296:CDS |
| GAATCCTGACAAAATGAAGA+AGG | 0.555649 | 5.4:-3835470 | MS.gene22296:CDS |
| GGTGGATAGTACCCAGGCAA+AGG | 0.583027 | 5.4:-3836304 | MS.gene22296:CDS |
| CGATAAGCTTCTTGAAGGAG+AGG | 0.587223 | 5.4:-3836922 | MS.gene22296:CDS |
| AATTTGAAAATTCTGCCTCA+AGG | 0.587627 | 5.4:+3836868 | None:intergenic |
| TTGAAGGAGAGGGGTATAGT+GGG | 0.600145 | 5.4:-3836911 | MS.gene22296:CDS |
| GGAAATTTCCATTAGCAAGG+TGG | 0.601010 | 5.4:-3836322 | MS.gene22296:CDS |
| CGCTTTCTAGTTGATATGCG+GGG | 0.601711 | 5.4:-3835895 | MS.gene22296:CDS |
| GTCAATCGAATTGGACCTTG+AGG | 0.607657 | 5.4:-3836883 | MS.gene22296:CDS |
| AATGAAGAAGGCTCAAGCAG+AGG | 0.609182 | 5.4:-3835458 | MS.gene22296:CDS |
| GATAAGCTTCTTGAAGGAGA+GGG | 0.631192 | 5.4:-3836921 | MS.gene22296:CDS |
| ATTCGGTGTTGGGTACGGGG+AGG | 0.658948 | 5.4:-3835432 | MS.gene22296:CDS |
| ATAAGCTTCTTGAAGGAGAG+GGG | 0.666842 | 5.4:-3836920 | MS.gene22296:CDS |
| GACGGCTGCAGTTCTTACAT+GGG | 0.670813 | 5.4:-3835643 | MS.gene22296:CDS |
| TAGCAAGGTGGATAGTACCC+AGG | 0.671253 | 5.4:-3836310 | MS.gene22296:CDS |
| TATTGCTGGTCATGAAACGA+CGG | 0.671671 | 5.4:-3835661 | MS.gene22296:CDS |
| AAATGCAAAAGAGAGTAGGG+AGG | 0.709740 | 5.4:-3836228 | MS.gene22296:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCATGAAAAGAAAACA+TGG | + | chr5.4:3836838-3836857 | None:intergenic | 20.0% |
| !! | GAATAATTATGTCATTATGT+GGG | - | chr5.4:3835762-3835781 | MS.gene22296:intron | 20.0% |
| !!! | TTTTGGATAACATACTAATA+TGG | + | chr5.4:3835700-3835719 | None:intergenic | 20.0% |
| !!! | TTTTTAAGCAATTCAAAAGT+TGG | + | chr5.4:3836966-3836985 | None:intergenic | 20.0% |
| ! | CTTTAGTCAAACCTTAATAA+CGG | + | chr5.4:3835596-3835615 | None:intergenic | 25.0% |
| ! | GGAATAATTATGTCATTATG+TGG | - | chr5.4:3835761-3835780 | MS.gene22296:intron | 25.0% |
| ! | TCATTAACTCTCTTAACATA+TGG | + | chr5.4:3835940-3835959 | None:intergenic | 25.0% |
| ! | TTTAGTCAAACCTTAATAAC+GGG | + | chr5.4:3835595-3835614 | None:intergenic | 25.0% |
| !! | TAGACAATATCTATCTTTTC+AGG | - | chr5.4:3836615-3836634 | MS.gene22296:intron | 25.0% |
| !! | TTGATTATACTTTTCTTTGC+AGG | - | chr5.4:3836268-3836287 | MS.gene22296:CDS | 25.0% |
| !!! | TAATACTTCGACTTTTTCAT+TGG | - | chr5.4:3836172-3836191 | MS.gene22296:intron | 25.0% |
| AAGGGATTACTCAAATTTGA+AGG | - | chr5.4:3836316-3836335 | MS.gene22296:CDS | 30.0% | |
| AATTTGAAAATTCTGCCTCA+AGG | + | chr5.4:3835507-3835526 | None:intergenic | 30.0% | |
| AATTTGGCGCTTGATATTAT+TGG | - | chr5.4:3835523-3835542 | MS.gene22296:intron | 30.0% | |
| ATTGGAAATTTCCATTAGCA+AGG | - | chr5.4:3836047-3836066 | MS.gene22296:intron | 30.0% | |
| ATTTGGCGCTTGATATTATT+GGG | - | chr5.4:3835524-3835543 | MS.gene22296:intron | 30.0% | |
| CATGTTCATGCAGTTTATTA+CGG | + | chr5.4:3835856-3835875 | None:intergenic | 30.0% | |
| CCAATATGGAATATAGAAAG+TGG | + | chr5.4:3836032-3836051 | None:intergenic | 30.0% | |
| CCACTTTCTATATTCCATAT+TGG | - | chr5.4:3836029-3836048 | MS.gene22296:intron | 30.0% | |
| CGTTATTAAGGTTTGACTAA+AGG | - | chr5.4:3835594-3835613 | MS.gene22296:intron | 30.0% | |
| CTGATTTATCATTCTTCCAA+AGG | - | chr5.4:3836534-3836553 | MS.gene22296:intron | 30.0% | |
| GAAATTGGTAAGCATTAAGA+TGG | + | chr5.4:3836873-3836892 | None:intergenic | 30.0% | |
| GACAAGTATTAATGACCTTA+AGG | + | chr5.4:3836108-3836127 | None:intergenic | 30.0% | |
| GCTAATGGAAATTTCCAATA+TGG | + | chr5.4:3836046-3836065 | None:intergenic | 30.0% | |
| GTAACAGATTTAACAGTAGA+GGG | + | chr5.4:3835970-3835989 | None:intergenic | 30.0% | |
| TAATACTGTTCTTTCTGAAC+AGG | + | chr5.4:3835425-3835444 | None:intergenic | 30.0% | |
| TCTACTGTTAAATCTGTTAC+AGG | - | chr5.4:3835970-3835989 | MS.gene22296:intron | 30.0% | |
| TGTAACAGATTTAACAGTAG+AGG | + | chr5.4:3835971-3835990 | None:intergenic | 30.0% | |
| TTAATGACAATGCTTATTGC+TGG | - | chr5.4:3836697-3836716 | MS.gene22296:intron | 30.0% | |
| TTCCATACTGTTTACTAAGA+TGG | - | chr5.4:3836429-3836448 | MS.gene22296:intron | 30.0% | |
| TTCCATCTTAGTAAACAGTA+TGG | + | chr5.4:3836434-3836453 | None:intergenic | 30.0% | |
| ! | AAATTTCGGTTGCTTTAGAT+AGG | - | chr5.4:3836571-3836590 | MS.gene22296:intron | 30.0% |
| ! | CATACAAAAGATTGAGCTTT+TGG | + | chr5.4:3835717-3835736 | None:intergenic | 30.0% |
| ! | CTTTGGAAGAATGATAAATC+AGG | + | chr5.4:3836536-3836555 | None:intergenic | 30.0% |
| ! | TCAGGTAGCTTTTCAAATTA+GGG | - | chr5.4:3836633-3836652 | MS.gene22296:intron | 30.0% |
| ! | TGAGGCAGAATTTTCAAATT+TGG | - | chr5.4:3835507-3835526 | MS.gene22296:intron | 30.0% |
| ! | TTCAGGTAGCTTTTCAAATT+AGG | - | chr5.4:3836632-3836651 | MS.gene22296:intron | 30.0% |
| ! | TTTTATGGATCTTACACCAA+GGG | - | chr5.4:3836356-3836375 | MS.gene22296:CDS | 30.0% |
| !! | TATCAAACTCCTAGTTTTCA+TGG | + | chr5.4:3836407-3836426 | None:intergenic | 30.0% |
| AACCAGTCTGGTTGAAATTT+CGG | - | chr5.4:3836557-3836576 | MS.gene22296:intron | 35.0% | |
| AAGATCGATAAGCTTCTTGA+AGG | - | chr5.4:3835445-3835464 | MS.gene22296:CDS | 35.0% | |
| AGAAAATATCTCTGAGCACT+AGG | + | chr5.4:3835740-3835759 | None:intergenic | 35.0% | |
| ATTAACATACTTGAGCTAGC+AGG | + | chr5.4:3836762-3836781 | None:intergenic | 35.0% | |
| CAATGAATCTCCCGTTATTA+AGG | - | chr5.4:3835582-3835601 | MS.gene22296:intron | 35.0% | |
| CAGAAATGCAAAAGAGAGTA+GGG | - | chr5.4:3836141-3836160 | MS.gene22296:intron | 35.0% | |
| GAATCCTGACAAAATGAAGA+AGG | - | chr5.4:3836902-3836921 | MS.gene22296:CDS | 35.0% | |
| GATAGGCAAAAATGCTTGTT+TGG | - | chr5.4:3836588-3836607 | MS.gene22296:intron | 35.0% | |
| GTCATTAATACTTGTCTCGA+TGG | - | chr5.4:3836112-3836131 | MS.gene22296:intron | 35.0% | |
| TCAGAAATGCAAAAGAGAGT+AGG | - | chr5.4:3836140-3836159 | MS.gene22296:intron | 35.0% | |
| TTACTAAGATGGAAGCTTAC+AGG | - | chr5.4:3836440-3836459 | MS.gene22296:intron | 35.0% | |
| ! | AGTGCTCAGAGATATTTTCT+GGG | - | chr5.4:3835740-3835759 | MS.gene22296:intron | 35.0% |
| ! | CTTAATAACGGGAGATTCAT+TGG | + | chr5.4:3835584-3835603 | None:intergenic | 35.0% |
| ! | CTTTTATGGATCTTACACCA+AGG | - | chr5.4:3836355-3836374 | MS.gene22296:CDS | 35.0% |
| ! | TAGTGCTCAGAGATATTTTC+TGG | - | chr5.4:3835739-3835758 | MS.gene22296:intron | 35.0% |
| !!! | CACTTTTTGAAGCTGAACAT+AGG | - | chr5.4:3836005-3836024 | MS.gene22296:intron | 35.0% |
| AAATGCAAAAGAGAGTAGGG+AGG | - | chr5.4:3836144-3836163 | MS.gene22296:intron | 40.0% | |
| AACCGAAATTTCAACCAGAC+TGG | + | chr5.4:3836562-3836581 | None:intergenic | 40.0% | |
| AATCTGTTACAGGCAGTGTA+TGG | - | chr5.4:3835980-3835999 | MS.gene22296:intron | 40.0% | |
| AGGATGACTCCATGAAAACT+AGG | - | chr5.4:3836395-3836414 | MS.gene22296:intron | 40.0% | |
| AGGGATAATGTTAAGCTCTC+AGG | - | chr5.4:3836375-3836394 | MS.gene22296:CDS | 40.0% | |
| ATAAGCTTCTTGAAGGAGAG+GGG | - | chr5.4:3835452-3835471 | MS.gene22296:CDS | 40.0% | |
| ATGTTGAGAAACTGCAGCAA+AGG | - | chr5.4:3836297-3836316 | MS.gene22296:CDS | 40.0% | |
| GAAGTTTCAGGATGACCTTA+AGG | - | chr5.4:3836090-3836109 | MS.gene22296:intron | 40.0% | |
| GAGAGCTTAACATTATCCCT+TGG | + | chr5.4:3836375-3836394 | None:intergenic | 40.0% | |
| GATAAGCTTCTTGAAGGAGA+GGG | - | chr5.4:3835451-3835470 | MS.gene22296:CDS | 40.0% | |
| GGAAATTTCCATTAGCAAGG+TGG | - | chr5.4:3836050-3836069 | MS.gene22296:intron | 40.0% | |
| GGATTCTGCAGTAGAGAAAT+TGG | + | chr5.4:3836888-3836907 | None:intergenic | 40.0% | |
| GGTGTGTTCAACTATGACTT+TGG | - | chr5.4:3835550-3835569 | MS.gene22296:intron | 40.0% | |
| TATTGCTGGTCATGAAACGA+CGG | - | chr5.4:3836711-3836730 | MS.gene22296:intron | 40.0% | |
| TGTTGAGAAACTGCAGCAAA+GGG | - | chr5.4:3836298-3836317 | MS.gene22296:CDS | 40.0% | |
| TTACGAGTCATTGCAGGAAT+TGG | - | chr5.4:3836198-3836217 | MS.gene22296:intron | 40.0% | |
| TTCCTTCTCTTGACAGTTGA+GGG | - | chr5.4:3836669-3836688 | MS.gene22296:intron | 40.0% | |
| TTTCCTTCTCTTGACAGTTG+AGG | - | chr5.4:3836668-3836687 | MS.gene22296:intron | 40.0% | |
| ! | TGAGCCTTCTTCATTTTGTC+AGG | + | chr5.4:3836909-3836928 | None:intergenic | 40.0% |
| !! | AGTTGATGTTGATGATCGTC+AGG | - | chr5.4:3836500-3836519 | MS.gene22296:intron | 40.0% |
| !! | TGCGCTTTCTAGTTGATATG+CGG | - | chr5.4:3836475-3836494 | MS.gene22296:intron | 40.0% |
| !! | TGTGTTGACTGTACATGACA+TGG | - | chr5.4:3836243-3836262 | MS.gene22296:CDS | 40.0% |
| AATGAAGAAGGCTCAAGCAG+AGG | - | chr5.4:3836914-3836933 | MS.gene22296:CDS | 45.0% | |
| ATCCCTCAACTGTCAAGAGA+AGG | + | chr5.4:3836674-3836693 | None:intergenic | 45.0% | |
| ATCCTGAAACTTCCTTTGCC+TGG | + | chr5.4:3836083-3836102 | None:intergenic | 45.0% | |
| CGATAAGCTTCTTGAAGGAG+AGG | - | chr5.4:3835450-3835469 | MS.gene22296:CDS | 45.0% | |
| GCGCTTGATATTATTGGGCT+AGG | - | chr5.4:3835529-3835548 | MS.gene22296:intron | 45.0% | |
| GGTACTATCCACCTTGCTAA+TGG | + | chr5.4:3836061-3836080 | None:intergenic | 45.0% | |
| GTCAATCGAATTGGACCTTG+AGG | - | chr5.4:3835489-3835508 | MS.gene22296:intron | 45.0% | |
| TCCTGAAACTTCCTTTGCCT+GGG | + | chr5.4:3836082-3836101 | None:intergenic | 45.0% | |
| TGAACATGACTGCTCATGAC+AGG | - | chr5.4:3836792-3836811 | MS.gene22296:CDS | 45.0% | |
| TGGGCAGAAGTCAATCGAAT+TGG | - | chr5.4:3835480-3835499 | MS.gene22296:intron | 45.0% | |
| TGGTGATTACGAGTCATTGC+AGG | - | chr5.4:3836192-3836211 | MS.gene22296:intron | 45.0% | |
| TGTCATGAGCAGTCATGTTC+AGG | + | chr5.4:3836793-3836812 | None:intergenic | 45.0% | |
| TTCTTCCAAAGGAACCAGTC+TGG | - | chr5.4:3836545-3836564 | MS.gene22296:intron | 45.0% | |
| ! | TTCAACCAGACTGGTTCCTT+TGG | + | chr5.4:3836553-3836572 | None:intergenic | 45.0% |
| ! | TTGAAGGAGAGGGGTATAGT+GGG | - | chr5.4:3835461-3835480 | MS.gene22296:CDS | 45.0% |
| !! | CGCTTTCTAGTTGATATGCG+GGG | - | chr5.4:3836477-3836496 | MS.gene22296:intron | 45.0% |
| !! | GCGCTTTCTAGTTGATATGC+GGG | - | chr5.4:3836476-3836495 | MS.gene22296:intron | 45.0% |
| !!! | ATTTTTTTTTTTATCTTTTA+TGG | - | chr5.4:3836341-3836360 | MS.gene22296:CDS | 5.0% |
| ACCCAGGCAAAGGAAGTTTC+AGG | - | chr5.4:3836078-3836097 | MS.gene22296:CDS | 50.0% | |
| GACAGGCAGATTTGTGGCAT+TGG | - | chr5.4:3836809-3836828 | MS.gene22296:CDS | 50.0% | |
| GACGGCTGCAGTTCTTACAT+GGG | - | chr5.4:3836729-3836748 | MS.gene22296:intron | 50.0% | |
| GCTCATGACAGGCAGATTTG+TGG | - | chr5.4:3836803-3836822 | MS.gene22296:CDS | 50.0% | |
| GGCTCAAGCAGAGGTAGATT+CGG | - | chr5.4:3836923-3836942 | MS.gene22296:CDS | 50.0% | |
| TAGCAAGGTGGATAGTACCC+AGG | - | chr5.4:3836062-3836081 | MS.gene22296:CDS | 50.0% | |
| ! | CTTGAAGGAGAGGGGTATAG+TGG | - | chr5.4:3835460-3835479 | MS.gene22296:CDS | 50.0% |
| !! | AGCAGAGGTAGATTCGGTGT+TGG | - | chr5.4:3836929-3836948 | MS.gene22296:CDS | 50.0% |
| !! | GCAGAGGTAGATTCGGTGTT+GGG | - | chr5.4:3836930-3836949 | MS.gene22296:CDS | 50.0% |
| !! | GGTAGATTCGGTGTTGGGTA+CGG | - | chr5.4:3836935-3836954 | MS.gene22296:CDS | 50.0% |
| !! | GTAGATTCGGTGTTGGGTAC+GGG | - | chr5.4:3836936-3836955 | MS.gene22296:CDS | 50.0% |
| !! | TAGATTCGGTGTTGGGTACG+GGG | - | chr5.4:3836937-3836956 | MS.gene22296:CDS | 50.0% |
| CGACGGCTGCAGTTCTTACA+TGG | - | chr5.4:3836728-3836747 | MS.gene22296:intron | 55.0% | |
| GGTGGATAGTACCCAGGCAA+AGG | - | chr5.4:3836068-3836087 | MS.gene22296:CDS | 55.0% | |
| !! | ATTCGGTGTTGGGTACGGGG+AGG | - | chr5.4:3836940-3836959 | MS.gene22296:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 3835403 | 3836991 | 3835403 | ID=MS.gene22296 |
| chr5.4 | mRNA | 3835403 | 3836991 | 3835403 | ID=MS.gene22296.t1;Parent=MS.gene22296 |
| chr5.4 | exon | 3836791 | 3836991 | 3836791 | ID=MS.gene22296.t1.exon1;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3836791 | 3836991 | 3836791 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| chr5.4 | exon | 3836229 | 3836402 | 3836229 | ID=MS.gene22296.t1.exon2;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3836229 | 3836402 | 3836229 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| chr5.4 | exon | 3836057 | 3836104 | 3836057 | ID=MS.gene22296.t1.exon3;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3836057 | 3836104 | 3836057 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| chr5.4 | exon | 3835873 | 3835932 | 3835873 | ID=MS.gene22296.t1.exon4;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3835873 | 3835932 | 3835873 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| chr5.4 | exon | 3835623 | 3835709 | 3835623 | ID=MS.gene22296.t1.exon5;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3835623 | 3835709 | 3835623 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| chr5.4 | exon | 3835403 | 3835491 | 3835403 | ID=MS.gene22296.t1.exon6;Parent=MS.gene22296.t1 |
| chr5.4 | CDS | 3835403 | 3835491 | 3835403 | ID=cds.MS.gene22296.t1;Parent=MS.gene22296.t1 |
| Gene Sequence |
| Protein sequence |
