Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89914.t1 | GAU13942.1 | 84.7 | 262 | 38 | 2 | 1 | 260 | 1 | 262 | 2.00E-81 | 312.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89914.t1 | A0A2K3NBC7 | 81.7 | 263 | 45 | 2 | 1 | 260 | 1 | 263 | 5.9e-75 | 290.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene89914 | 0.838509 | 2.76E-57 | -1.69E-46 |
| MS.gene049346 | MS.gene89914 | 0.825715 | 4.03E-54 | -1.69E-46 |
| MS.gene049351 | MS.gene89914 | 0.877775 | 4.79E-69 | -1.69E-46 |
| MS.gene049427 | MS.gene89914 | 0.866683 | 2.38E-65 | -1.69E-46 |
| MS.gene049491 | MS.gene89914 | 0.818031 | 2.42E-52 | -1.69E-46 |
| MS.gene049661 | MS.gene89914 | 0.874415 | 6.88E-68 | -1.69E-46 |
| MS.gene049662 | MS.gene89914 | 0.874092 | 8.84E-68 | -1.69E-46 |
| MS.gene049758 | MS.gene89914 | 0.863385 | 2.59E-64 | -1.69E-46 |
| MS.gene050076 | MS.gene89914 | 0.892673 | 1.28E-74 | -1.69E-46 |
| MS.gene050099 | MS.gene89914 | 0.829839 | 4.11E-55 | -1.69E-46 |
| MS.gene050231 | MS.gene89914 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene89914 | 0.901418 | 2.74E-78 | -1.69E-46 |
| MS.gene050397 | MS.gene89914 | 0.881408 | 2.46E-70 | -1.69E-46 |
| MS.gene050404 | MS.gene89914 | 0.834293 | 3.26E-56 | -1.69E-46 |
| MS.gene050406 | MS.gene89914 | 0.856085 | 4.08E-62 | -1.69E-46 |
| MS.gene050432 | MS.gene89914 | 0.85978 | 3.26E-63 | -1.69E-46 |
| MS.gene050464 | MS.gene89914 | 0.864027 | 1.63E-64 | -1.69E-46 |
| MS.gene050529 | MS.gene89914 | 0.886996 | 2.10E-72 | -1.69E-46 |
| MS.gene050670 | MS.gene89914 | 0.85142 | 8.98E-61 | -1.69E-46 |
| MS.gene050767 | MS.gene89914 | 0.816794 | 4.60E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene89914 | 0.818383 | 2.02E-52 | -1.69E-46 |
| MS.gene050783 | MS.gene89914 | 0.861077 | 1.32E-63 | -1.69E-46 |
| MS.gene050812 | MS.gene89914 | 0.842445 | 2.58E-58 | -1.69E-46 |
| MS.gene05101 | MS.gene89914 | 0.896098 | 5.11E-76 | -1.69E-46 |
| MS.gene05105 | MS.gene89914 | 0.831365 | 1.74E-55 | -1.69E-46 |
| MS.gene051242 | MS.gene89914 | 0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene89914 | 0.824613 | 7.34E-54 | -1.69E-46 |
| MS.gene051471 | MS.gene89914 | 0.877212 | 7.53E-69 | -1.69E-46 |
| MS.gene051525 | MS.gene89914 | 0.802584 | 5.23E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene89914 | 0.88084 | 3.94E-70 | -1.69E-46 |
| MS.gene051662 | MS.gene89914 | 0.806746 | 7.08E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene89914 | 0.829088 | 6.26E-55 | -1.69E-46 |
| MS.gene051860 | MS.gene89914 | 0.825926 | 3.59E-54 | -1.69E-46 |
| MS.gene051877 | MS.gene89914 | 0.837861 | 4.05E-57 | -1.69E-46 |
| MS.gene052011 | MS.gene89914 | -0.835706 | 1.44E-56 | -1.69E-46 |
| MS.gene052077 | MS.gene89914 | 0.803859 | 2.85E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene89914 | 0.884002 | 2.78E-71 | -1.69E-46 |
| MS.gene052178 | MS.gene89914 | 0.833941 | 3.99E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene89914 | 0.857648 | 1.41E-62 | -1.69E-46 |
| MS.gene052207 | MS.gene89914 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene052253 | MS.gene89914 | 0.855803 | 4.94E-62 | -1.69E-46 |
| MS.gene052290 | MS.gene89914 | 0.801682 | 8.02E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene89914 | 0.885745 | 6.24E-72 | -1.69E-46 |
| MS.gene052322 | MS.gene89914 | 0.819109 | 1.38E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene89914 | 0.884323 | 2.11E-71 | -1.69E-46 |
| MS.gene052340 | MS.gene89914 | 0.848 | 8.10E-60 | -1.69E-46 |
| MS.gene052421 | MS.gene89914 | 0.808198 | 3.48E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene89914 | 0.821094 | 4.85E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene89914 | 0.83698 | 6.81E-57 | -1.69E-46 |
| MS.gene052767 | MS.gene89914 | 0.804268 | 2.34E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene89914 | 0.812026 | 5.22E-51 | -1.69E-46 |
| MS.gene053107 | MS.gene89914 | 0.840228 | 9.87E-58 | -1.69E-46 |
| MS.gene053209 | MS.gene89914 | 0.828156 | 1.05E-54 | -1.69E-46 |
| MS.gene053322 | MS.gene89914 | 0.833731 | 4.51E-56 | -1.69E-46 |
| MS.gene053557 | MS.gene89914 | 0.840592 | 7.93E-58 | -1.69E-46 |
| MS.gene053836 | MS.gene89914 | 0.897887 | 9.09E-77 | -1.69E-46 |
| MS.gene053907 | MS.gene89914 | 0.860704 | 1.72E-63 | -1.69E-46 |
| MS.gene053928 | MS.gene89914 | 0.821359 | 4.21E-53 | -1.69E-46 |
| MS.gene053971 | MS.gene89914 | 0.873413 | 1.50E-67 | -1.69E-46 |
| MS.gene054010 | MS.gene89914 | 0.800933 | 1.14E-48 | -1.69E-46 |
| MS.gene054011 | MS.gene89914 | 0.813801 | 2.13E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene89914 | 0.805777 | 1.13E-49 | -1.69E-46 |
| MS.gene054013 | MS.gene89914 | 0.803255 | 3.80E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene89914 | 0.857292 | 1.80E-62 | -1.69E-46 |
| MS.gene054183 | MS.gene89914 | 0.811918 | 5.51E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene89914 | 0.849868 | 2.45E-60 | -1.69E-46 |
| MS.gene054473 | MS.gene89914 | 0.839812 | 1.27E-57 | -1.69E-46 |
| MS.gene05455 | MS.gene89914 | 0.860314 | 2.25E-63 | -1.69E-46 |
| MS.gene054719 | MS.gene89914 | 0.813162 | 2.94E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene89914 | 0.865393 | 6.10E-65 | -1.69E-46 |
| MS.gene054803 | MS.gene89914 | 0.86586 | 4.35E-65 | -1.69E-46 |
| MS.gene054804 | MS.gene89914 | 0.866677 | 2.39E-65 | -1.69E-46 |
| MS.gene054983 | MS.gene89914 | 0.841577 | 4.37E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene89914 | 0.87612 | 1.80E-68 | -1.69E-46 |
| MS.gene055277 | MS.gene89914 | 0.908397 | 1.79E-81 | -1.69E-46 |
| MS.gene055390 | MS.gene89914 | 0.87278 | 2.45E-67 | -1.69E-46 |
| MS.gene055397 | MS.gene89914 | 0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene055412 | MS.gene89914 | 0.822906 | 1.84E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene89914 | 0.894703 | 1.92E-75 | -1.69E-46 |
| MS.gene055428 | MS.gene89914 | 0.904421 | 1.25E-79 | -1.69E-46 |
| MS.gene055499 | MS.gene89914 | 0.826396 | 2.78E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene89914 | 0.843332 | 1.50E-58 | -1.69E-46 |
| MS.gene055564 | MS.gene89914 | 0.819793 | 9.64E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene89914 | 0.849299 | 3.53E-60 | -1.69E-46 |
| MS.gene055712 | MS.gene89914 | 0.831604 | 1.52E-55 | -1.69E-46 |
| MS.gene055896 | MS.gene89914 | 0.832187 | 1.09E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene89914 | 0.848014 | 8.03E-60 | -1.69E-46 |
| MS.gene056407 | MS.gene89914 | 0.89501 | 1.44E-75 | -1.69E-46 |
| MS.gene056410 | MS.gene89914 | 0.813942 | 1.98E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene89914 | 0.847102 | 1.43E-59 | -1.69E-46 |
| MS.gene056452 | MS.gene89914 | 0.827183 | 1.80E-54 | -1.69E-46 |
| MS.gene056609 | MS.gene89914 | 0.82248 | 2.32E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene89914 | 0.899437 | 1.98E-77 | -1.69E-46 |
| MS.gene056659 | MS.gene89914 | 0.835023 | 2.14E-56 | -1.69E-46 |
| MS.gene056723 | MS.gene89914 | 0.829445 | 5.13E-55 | -1.69E-46 |
| MS.gene056796 | MS.gene89914 | 0.833903 | 4.08E-56 | -1.69E-46 |
| MS.gene056846 | MS.gene89914 | 0.806656 | 7.40E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene89914 | -0.84128 | 5.23E-58 | -1.69E-46 |
| MS.gene057011 | MS.gene89914 | 0.854829 | 9.48E-62 | -1.69E-46 |
| MS.gene05702 | MS.gene89914 | 0.904307 | 1.41E-79 | -1.69E-46 |
| MS.gene057121 | MS.gene89914 | 0.879586 | 1.10E-69 | -1.69E-46 |
| MS.gene057123 | MS.gene89914 | 0.827036 | 1.95E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene89914 | 0.821345 | 4.24E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene89914 | 0.809174 | 2.16E-50 | -1.69E-46 |
| MS.gene057146 | MS.gene89914 | 0.838803 | 2.32E-57 | -1.69E-46 |
| MS.gene057245 | MS.gene89914 | 0.855603 | 5.65E-62 | -1.69E-46 |
| MS.gene057295 | MS.gene89914 | 0.81515 | 1.07E-51 | -1.69E-46 |
| MS.gene05733 | MS.gene89914 | 0.823012 | 1.74E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene89914 | 0.840988 | 6.24E-58 | -1.69E-46 |
| MS.gene057450 | MS.gene89914 | 0.806547 | 7.80E-50 | -1.69E-46 |
| MS.gene057476 | MS.gene89914 | 0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene89914 | 0.842876 | 1.98E-58 | -1.69E-46 |
| MS.gene057823 | MS.gene89914 | 0.836786 | 7.63E-57 | -1.69E-46 |
| MS.gene057845 | MS.gene89914 | 0.822021 | 2.96E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene89914 | 0.89211 | 2.15E-74 | -1.69E-46 |
| MS.gene058019 | MS.gene89914 | 0.815767 | 7.81E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene89914 | 0.826727 | 2.31E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene89914 | 0.868603 | 5.78E-66 | -1.69E-46 |
| MS.gene058142 | MS.gene89914 | 0.854859 | 9.30E-62 | -1.69E-46 |
| MS.gene058233 | MS.gene89914 | 0.91231 | 2.25E-83 | -1.69E-46 |
| MS.gene058276 | MS.gene89914 | 0.849951 | 2.33E-60 | -1.69E-46 |
| MS.gene058293 | MS.gene89914 | 0.868819 | 4.92E-66 | -1.69E-46 |
| MS.gene058365 | MS.gene89914 | 0.846807 | 1.72E-59 | -1.69E-46 |
| MS.gene058660 | MS.gene89914 | 0.917545 | 4.66E-86 | -1.69E-46 |
| MS.gene058710 | MS.gene89914 | 0.80556 | 1.26E-49 | -1.69E-46 |
| MS.gene058749 | MS.gene89914 | 0.811136 | 8.14E-51 | -1.69E-46 |
| MS.gene058787 | MS.gene89914 | 0.826924 | 2.08E-54 | -1.69E-46 |
| MS.gene058845 | MS.gene89914 | 0.840842 | 6.82E-58 | -1.69E-46 |
| MS.gene05886 | MS.gene89914 | 0.831468 | 1.64E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene89914 | 0.81194 | 5.44E-51 | -1.69E-46 |
| MS.gene058993 | MS.gene89914 | 0.813035 | 3.14E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene89914 | 0.811639 | 6.33E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene89914 | 0.895963 | 5.81E-76 | -1.69E-46 |
| MS.gene059141 | MS.gene89914 | 0.82785 | 1.25E-54 | -1.69E-46 |
| MS.gene059294 | MS.gene89914 | 0.824104 | 9.67E-54 | -1.69E-46 |
| MS.gene059326 | MS.gene89914 | 0.81121 | 7.84E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene89914 | 0.834802 | 2.43E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene89914 | 0.854401 | 1.26E-61 | -1.69E-46 |
| MS.gene059536 | MS.gene89914 | 0.832298 | 1.02E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene89914 | 0.874197 | 8.15E-68 | -1.69E-46 |
| MS.gene05959 | MS.gene89914 | 0.82559 | 4.31E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene89914 | 0.854487 | 1.19E-61 | -1.69E-46 |
| MS.gene059688 | MS.gene89914 | 0.863338 | 2.67E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene89914 | 0.871501 | 6.51E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene89914 | 0.877075 | 8.40E-69 | -1.69E-46 |
| MS.gene059708 | MS.gene89914 | 0.807559 | 4.77E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene89914 | 0.854198 | 1.44E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene89914 | 0.871096 | 8.87E-67 | -1.69E-46 |
| MS.gene059960 | MS.gene89914 | 0.844924 | 5.59E-59 | -1.69E-46 |
| MS.gene06037 | MS.gene89914 | 0.857557 | 1.50E-62 | -1.69E-46 |
| MS.gene060403 | MS.gene89914 | 0.829807 | 4.19E-55 | -1.69E-46 |
| MS.gene060407 | MS.gene89914 | 0.854406 | 1.26E-61 | -1.69E-46 |
| MS.gene060804 | MS.gene89914 | 0.829398 | 5.27E-55 | -1.69E-46 |
| MS.gene06090 | MS.gene89914 | 0.831801 | 1.36E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene89914 | 0.904706 | 9.27E-80 | -1.69E-46 |
| MS.gene061051 | MS.gene89914 | -0.845506 | 3.89E-59 | -1.69E-46 |
| MS.gene061112 | MS.gene89914 | 0.806169 | 9.37E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene89914 | 0.814341 | 1.62E-51 | -1.69E-46 |
| MS.gene061159 | MS.gene89914 | 0.808707 | 2.71E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene89914 | 0.818575 | 1.82E-52 | -1.69E-46 |
| MS.gene061265 | MS.gene89914 | 0.805619 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89914.t1 | MTR_4g015010 | 93.916 | 263 | 12 | 3 | 1 | 260 | 14 | 275 | 0.0 | 500 |
| MS.gene89914.t1 | MTR_7g067000 | 60.714 | 252 | 84 | 8 | 1 | 250 | 1 | 239 | 9.84e-84 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89914.t1 | AT3G09050 | 52.263 | 243 | 101 | 5 | 16 | 249 | 7 | 243 | 4.04e-73 | 224 |
Find 38 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCGTCTTACGGATTATT+TGG | 0.131531 | 4.2:+86456663 | MS.gene89914:CDS |
| ATTGGTGAAGATGCTCTTAA+AGG | 0.254433 | 4.2:+86456745 | MS.gene89914:CDS |
| AATGTTTACTTATTCTTCAT+TGG | 0.294155 | 4.2:+86458058 | MS.gene89914:CDS |
| TGGAAGAAAGTTGCAGTTCT+TGG | 0.299282 | 4.2:+86458139 | MS.gene89914:CDS |
| CCAGGTTGTGAAGTATTATC+AGG | 0.334032 | 4.2:-86456583 | None:intergenic |
| GTTGATAGAAGAAGGATAGA+AGG | 0.335400 | 4.2:-86456473 | None:intergenic |
| GTAACAACAAATGAGGAAAA+TGG | 0.350272 | 4.2:+86458019 | MS.gene89914:CDS |
| TCTGAATACTTGACACAGTT+TGG | 0.366087 | 4.2:+86456694 | MS.gene89914:CDS |
| GAGTGAGATGCTAGCATTTG+AGG | 0.367845 | 4.2:+86457823 | MS.gene89914:CDS |
| TTCTTCTTGTGTTGTGGTGG+GGG | 0.383441 | 4.2:-86456519 | None:intergenic |
| ATAGAAGGAAGGGTGAAATA+TGG | 0.405812 | 4.2:-86456458 | None:intergenic |
| CAAGTTGAGCTAGTAGCTCT+TGG | 0.422521 | 4.2:-86456621 | None:intergenic |
| ACTTCTTCTTGTGTTGTGGT+GGG | 0.425298 | 4.2:-86456521 | None:intergenic |
| CTTCTTCTTGTGTTGTGGTG+GGG | 0.430419 | 4.2:-86456520 | None:intergenic |
| AGTATTATCAGGTTCATCAT+TGG | 0.435688 | 4.2:-86456572 | None:intergenic |
| AATGGAGATTGAAGGACAAA+TGG | 0.440803 | 4.2:+86457895 | MS.gene89914:CDS |
| GGACTATTCTTTAAGAACTT+AGG | 0.444508 | 4.2:+86457935 | MS.gene89914:CDS |
| AACTTCTTCTTGTGTTGTGG+TGG | 0.447722 | 4.2:-86456522 | None:intergenic |
| CATCATTAAGTAGTACTGAT+TGG | 0.456180 | 4.2:+86458119 | MS.gene89914:CDS |
| ACTAGACAGAGTAGAGTTTG+AGG | 0.456846 | 4.2:+86457856 | MS.gene89914:CDS |
| TGGCTTTGAACATCACCTCC+AGG | 0.458846 | 4.2:-86456601 | None:intergenic |
| AAAGCTGAAATTGATAAGAT+TGG | 0.462568 | 4.2:+86456727 | MS.gene89914:CDS |
| TTTAAGAACTTAGGACAGAA+AGG | 0.478730 | 4.2:+86457944 | MS.gene89914:CDS |
| ATAGAAGAAGGATAGAAGGA+AGG | 0.483138 | 4.2:-86456469 | None:intergenic |
| ATCAGGTTCATCATTGGAGT+TGG | 0.486180 | 4.2:-86456566 | None:intergenic |
| CAGAACTTCTTCTTGTGTTG+TGG | 0.493087 | 4.2:-86456525 | None:intergenic |
| GGAAAGTGAAAGCAAAGTAA+TGG | 0.500197 | 4.2:+86457877 | MS.gene89914:CDS |
| TAGAAGAAGGATAGAAGGAA+GGG | 0.500325 | 4.2:-86456468 | None:intergenic |
| TGAAGATGCTCTTAAAGGAT+TGG | 0.511035 | 4.2:+86456750 | MS.gene89914:CDS |
| ATGGAAGTTGACAGAAATGA+AGG | 0.525154 | 4.2:+86457914 | MS.gene89914:CDS |
| GTGGTGGTGTTGATAGAAGA+AGG | 0.543371 | 4.2:-86456481 | None:intergenic |
| GAAAGATGTAACAACAAATG+AGG | 0.557127 | 4.2:+86458012 | MS.gene89914:CDS |
| AGCAAAGTAATGGAGATTGA+AGG | 0.564863 | 4.2:+86457887 | MS.gene89914:CDS |
| CCTGATAATACTTCACAACC+TGG | 0.574595 | 4.2:+86456583 | MS.gene89914:CDS |
| GGGGTGCAGAGTAGTAAAAG+TGG | 0.581339 | 4.2:-86456500 | None:intergenic |
| AGTTCTTGGAGCTATTCTTG+TGG | 0.591041 | 4.2:+86458153 | MS.gene89914:CDS |
| GTGCAGAGTAGTAAAAGTGG+TGG | 0.674073 | 4.2:-86456497 | None:intergenic |
| GATAATACTTCACAACCTGG+AGG | 0.739356 | 4.2:+86456586 | MS.gene89914:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCTTATAAAAAAATA+TGG | - | chr4.2:86457563-86457582 | None:intergenic | 10.0% |
| !!! | ATACTTTAAAAACTGAATTA+TGG | - | chr4.2:86456900-86456919 | None:intergenic | 15.0% |
| !! | AATCACAAATGAATGTAAAA+GGG | - | chr4.2:86457781-86457800 | None:intergenic | 20.0% |
| !! | AATGTTTACTTATTCTTCAT+TGG | + | chr4.2:86458058-86458077 | MS.gene89914:CDS | 20.0% |
| !! | TAATCACAAATGAATGTAAA+AGG | - | chr4.2:86457782-86457801 | None:intergenic | 20.0% |
| ! | AAAACTGAATTATGGTTTAC+TGG | - | chr4.2:86456892-86456911 | None:intergenic | 25.0% |
| ! | AAAGCTGAAATTGATAAGAT+TGG | + | chr4.2:86456727-86456746 | MS.gene89914:CDS | 25.0% |
| ! | TACTTTGTACACTTATCATA+TGG | + | chr4.2:86457674-86457693 | MS.gene89914:intron | 25.0% |
| ! | TTAAAGTATAGTTCCTACTA+GGG | - | chr4.2:86457001-86457020 | None:intergenic | 25.0% |
| !! | CTTTTTACATATGGAATAGT+TGG | + | chr4.2:86458082-86458101 | MS.gene89914:CDS | 25.0% |
| !! | TTTAAAGTATAGTTCCTACT+AGG | - | chr4.2:86457002-86457021 | None:intergenic | 25.0% |
| !!! | CATCTTTTCTATTTCTTCTT+TGG | - | chr4.2:86457993-86458012 | None:intergenic | 25.0% |
| !!! | TTCATTGGTCTTTTTACATA+TGG | + | chr4.2:86458073-86458092 | MS.gene89914:CDS | 25.0% |
| AAACTGTAGTTTGAATCCTA+GGG | + | chr4.2:86457117-86457136 | MS.gene89914:intron | 30.0% | |
| AGATAAAATGAGAGAGAGAT+TGG | + | chr4.2:86457043-86457062 | MS.gene89914:intron | 30.0% | |
| AGGAAAATGGAAGAAAAACA+AGG | + | chr4.2:86458032-86458051 | MS.gene89914:CDS | 30.0% | |
| AGTATTATCAGGTTCATCAT+TGG | - | chr4.2:86456575-86456594 | None:intergenic | 30.0% | |
| ATTGGACTATAATGTTAGTC+AGG | + | chr4.2:86457061-86457080 | MS.gene89914:intron | 30.0% | |
| GAAAGATGTAACAACAAATG+AGG | + | chr4.2:86458012-86458031 | MS.gene89914:CDS | 30.0% | |
| GTAACAACAAATGAGGAAAA+TGG | + | chr4.2:86458019-86458038 | MS.gene89914:CDS | 30.0% | |
| TCATTATGTTAATCCCTAGT+AGG | + | chr4.2:86456985-86457004 | MS.gene89914:intron | 30.0% | |
| TTTAAGAACTTAGGACAGAA+AGG | + | chr4.2:86457944-86457963 | MS.gene89914:CDS | 30.0% | |
| ! | GCTTTTGCTTTATCAACATT+AGG | - | chr4.2:86457971-86457990 | None:intergenic | 30.0% |
| ! | GGACTATTCTTTAAGAACTT+AGG | + | chr4.2:86457935-86457954 | MS.gene89914:CDS | 30.0% |
| ! | TTGAGAATTAGTCTCATAGT+CGG | + | chr4.2:86457195-86457214 | MS.gene89914:intron | 30.0% |
| !! | CATCATTAAGTAGTACTGAT+TGG | + | chr4.2:86458119-86458138 | MS.gene89914:CDS | 30.0% |
| !!! | TTTGTTTTGTCAAGATGCTA+GGG | - | chr4.2:86457316-86457335 | None:intergenic | 30.0% |
| AACTGTAGTTTGAATCCTAG+GGG | + | chr4.2:86457118-86457137 | MS.gene89914:intron | 35.0% | |
| AATGGAGATTGAAGGACAAA+TGG | + | chr4.2:86457895-86457914 | MS.gene89914:CDS | 35.0% | |
| AGCTAACTAGAATCTATGCA+TGG | - | chr4.2:86456839-86456858 | None:intergenic | 35.0% | |
| ATAGAAGAAGGATAGAAGGA+AGG | - | chr4.2:86456472-86456491 | None:intergenic | 35.0% | |
| ATAGAAGGAAGGGTGAAATA+TGG | - | chr4.2:86456461-86456480 | None:intergenic | 35.0% | |
| ATGGAAGTTGACAGAAATGA+AGG | + | chr4.2:86457914-86457933 | MS.gene89914:CDS | 35.0% | |
| ATTGGTGAAGATGCTCTTAA+AGG | + | chr4.2:86456745-86456764 | MS.gene89914:CDS | 35.0% | |
| GAAACTGTAGTTTGAATCCT+AGG | + | chr4.2:86457116-86457135 | MS.gene89914:intron | 35.0% | |
| GACAGAAAAAGTTCGTCTTA+CGG | + | chr4.2:86456654-86456673 | MS.gene89914:CDS | 35.0% | |
| GGAAAGTGAAAGCAAAGTAA+TGG | + | chr4.2:86457877-86457896 | MS.gene89914:CDS | 35.0% | |
| TAGAAGAAGGATAGAAGGAA+GGG | - | chr4.2:86456471-86456490 | None:intergenic | 35.0% | |
| TCTGAATACTTGACACAGTT+TGG | + | chr4.2:86456694-86456713 | MS.gene89914:CDS | 35.0% | |
| TGAAGATGCTCTTAAAGGAT+TGG | + | chr4.2:86456750-86456769 | MS.gene89914:CDS | 35.0% | |
| ! | AGCAAAGTAATGGAGATTGA+AGG | + | chr4.2:86457887-86457906 | MS.gene89914:CDS | 35.0% |
| ! | AGTTCGTCTTACGGATTATT+TGG | + | chr4.2:86456663-86456682 | MS.gene89914:CDS | 35.0% |
| ! | GTTGATAGAAGAAGGATAGA+AGG | - | chr4.2:86456476-86456495 | None:intergenic | 35.0% |
| ! | TCTCCTCAAAGTCTCAATTT+TGG | - | chr4.2:86457420-86457439 | None:intergenic | 35.0% |
| !! | CTCAAAGTCTCAATTTTGGT+GGG | - | chr4.2:86457416-86457435 | None:intergenic | 35.0% |
| !! | GCATAAGTACTGCTTTTAAC+AGG | - | chr4.2:86457727-86457746 | None:intergenic | 35.0% |
| !!! | CTTTGTTTTGTCAAGATGCT+AGG | - | chr4.2:86457317-86457336 | None:intergenic | 35.0% |
| AACTTCTTCTTGTGTTGTGG+TGG | - | chr4.2:86456525-86456544 | None:intergenic | 40.0% | |
| ACTAGACAGAGTAGAGTTTG+AGG | + | chr4.2:86457856-86457875 | MS.gene89914:CDS | 40.0% | |
| ACTTCTTCTTGTGTTGTGGT+GGG | - | chr4.2:86456524-86456543 | None:intergenic | 40.0% | |
| AGTTCTTGGAGCTATTCTTG+TGG | + | chr4.2:86458153-86458172 | MS.gene89914:CDS | 40.0% | |
| AGTTTCTCAGATACTCCTAC+GGG | - | chr4.2:86457102-86457121 | None:intergenic | 40.0% | |
| ATCAGGTTCATCATTGGAGT+TGG | - | chr4.2:86456569-86456588 | None:intergenic | 40.0% | |
| CAGAACTTCTTCTTGTGTTG+TGG | - | chr4.2:86456528-86456547 | None:intergenic | 40.0% | |
| CAGTTTCTCAGATACTCCTA+CGG | - | chr4.2:86457103-86457122 | None:intergenic | 40.0% | |
| CCACCAAAATTGAGACTTTG+AGG | + | chr4.2:86457414-86457433 | MS.gene89914:intron | 40.0% | |
| CCTGATAATACTTCACAACC+TGG | + | chr4.2:86456583-86456602 | MS.gene89914:CDS | 40.0% | |
| GATAATACTTCACAACCTGG+AGG | + | chr4.2:86456586-86456605 | MS.gene89914:CDS | 40.0% | |
| GATGCTAGGGACTAATACAA+AGG | - | chr4.2:86457303-86457322 | None:intergenic | 40.0% | |
| GGACTATAATGTTAGTCAGG+TGG | + | chr4.2:86457064-86457083 | MS.gene89914:intron | 40.0% | |
| TGGAAGAAAGTTGCAGTTCT+TGG | + | chr4.2:86458139-86458158 | MS.gene89914:CDS | 40.0% | |
| ! | CCAGGTTGTGAAGTATTATC+AGG | - | chr4.2:86456586-86456605 | None:intergenic | 40.0% |
| ! | CCTCAAAGTCTCAATTTTGG+TGG | - | chr4.2:86457417-86457436 | None:intergenic | 40.0% |
| CTTCTTCTTGTGTTGTGGTG+GGG | - | chr4.2:86456523-86456542 | None:intergenic | 45.0% | |
| CTTTCGTTCTCAACCTCCAT+GGG | - | chr4.2:86457149-86457168 | None:intergenic | 45.0% | |
| GAGTGAGATGCTAGCATTTG+AGG | + | chr4.2:86457823-86457842 | MS.gene89914:CDS | 45.0% | |
| GGTCCAAATGACTGAAGTAG+CGG | + | chr4.2:86457216-86457235 | MS.gene89914:intron | 45.0% | |
| GTCCAAATGACTGAAGTAGC+GGG | + | chr4.2:86457217-86457236 | MS.gene89914:intron | 45.0% | |
| GTGCAGAGTAGTAAAAGTGG+TGG | - | chr4.2:86456500-86456519 | None:intergenic | 45.0% | |
| GTTTCTCAGATACTCCTACG+GGG | - | chr4.2:86457101-86457120 | None:intergenic | 45.0% | |
| TCCAAATGACTGAAGTAGCG+GGG | + | chr4.2:86457218-86457237 | MS.gene89914:intron | 45.0% | |
| TTCTTCTTGTGTTGTGGTGG+GGG | - | chr4.2:86456522-86456541 | None:intergenic | 45.0% | |
| ! | CAAGTTGAGCTAGTAGCTCT+TGG | - | chr4.2:86456624-86456643 | None:intergenic | 45.0% |
| !! | GTGGTGGTGTTGATAGAAGA+AGG | - | chr4.2:86456484-86456503 | None:intergenic | 45.0% |
| AATCCTAGGGGAGTAACCCA+TGG | + | chr4.2:86457130-86457149 | MS.gene89914:intron | 50.0% | |
| CCATGGAGGTTGAGAACGAA+AGG | + | chr4.2:86457147-86457166 | MS.gene89914:intron | 50.0% | |
| CCTTTCGTTCTCAACCTCCA+TGG | - | chr4.2:86457150-86457169 | None:intergenic | 50.0% | |
| CTCACCGTGATTAGAGCCTT+TGG | - | chr4.2:86457269-86457288 | None:intergenic | 50.0% | |
| GGGGTGCAGAGTAGTAAAAG+TGG | - | chr4.2:86456503-86456522 | None:intergenic | 50.0% | |
| TCCCCGCTACTTCAGTCATT+TGG | - | chr4.2:86457222-86457241 | None:intergenic | 50.0% | |
| TGGAAGCAATTTGACCCCGT+AGG | + | chr4.2:86457084-86457103 | MS.gene89914:intron | 50.0% | |
| ! | GGTGCCAAAGGCTCTAATCA+CGG | + | chr4.2:86457262-86457281 | MS.gene89914:intron | 50.0% |
| ! | TGGACTCATTGTGGTGCCAA+AGG | + | chr4.2:86457250-86457269 | MS.gene89914:intron | 50.0% |
| !! | ATGCCATGGTGGACTCATTG+TGG | + | chr4.2:86457241-86457260 | MS.gene89914:intron | 50.0% |
| !! | TGGCTTTGAACATCACCTCC+AGG | - | chr4.2:86456604-86456623 | None:intergenic | 50.0% |
| GCACCACAATGAGTCCACCA+TGG | - | chr4.2:86457247-86457266 | None:intergenic | 55.0% | |
| CCTAGGGGAGTAACCCATGG+AGG | + | chr4.2:86457133-86457152 | MS.gene89914:intron | 60.0% | |
| ! | AAGTAGCGGGGATGCCATGG+TGG | + | chr4.2:86457230-86457249 | MS.gene89914:intron | 60.0% |
| ! | CCTCCATGGGTTACTCCCCT+AGG | - | chr4.2:86457136-86457155 | None:intergenic | 60.0% |
| ! | CTGAAGTAGCGGGGATGCCA+TGG | + | chr4.2:86457227-86457246 | MS.gene89914:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 86456442 | 86458240 | 86456442 | ID=MS.gene89914 |
| chr4.2 | mRNA | 86456442 | 86458240 | 86456442 | ID=MS.gene89914.t1;Parent=MS.gene89914 |
| chr4.2 | exon | 86456442 | 86456789 | 86456442 | ID=MS.gene89914.t1.exon1;Parent=MS.gene89914.t1 |
| chr4.2 | CDS | 86456442 | 86456789 | 86456442 | ID=cds.MS.gene89914.t1;Parent=MS.gene89914.t1 |
| chr4.2 | exon | 86457806 | 86458240 | 86457806 | ID=MS.gene89914.t1.exon2;Parent=MS.gene89914.t1 |
| chr4.2 | CDS | 86457806 | 86458240 | 86457806 | ID=cds.MS.gene89914.t1;Parent=MS.gene89914.t1 |
| Gene Sequence |
| Protein sequence |
