AlfalfaGEDB | MS.gene77433

M. sativa cultivar XinJiangDaYe / MS.gene77433

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77433.t1	XP_003618640.1	59.6	480	38	1	1	324	1	480	1.90E-142	515.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77433.t1	F8WKW1	36.1	477	143	5	7	324	8	481	1.0e-70	268.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77433.t1	G7KLV2	59.6	480	38	1	1	324	1	480	1.4e-142	515.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene77433	0.83313	6.36E-56	-1.69E-46
MS.gene049170	MS.gene77433	0.809044	2.30E-50	-1.69E-46
MS.gene049351	MS.gene77433	0.833975	3.92E-56	-1.69E-46
MS.gene049356	MS.gene77433	0.831064	2.06E-55	-1.69E-46
MS.gene049369	MS.gene77433	0.810477	1.13E-50	-1.69E-46
MS.gene049427	MS.gene77433	0.820664	6.09E-53	-1.69E-46
MS.gene049488	MS.gene77433	0.817139	3.85E-52	-1.69E-46
MS.gene049609	MS.gene77433	0.84411	9.26E-59	-1.69E-46
MS.gene049661	MS.gene77433	0.824644	7.22E-54	-1.69E-46
MS.gene049662	MS.gene77433	0.814834	1.26E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77433.t1	MTR_6g013990	85.938	256	36	0	1	256	1	256	1.30e-154	442
MS.gene77433.t1	MTR_6g013990	97.059	68	2	0	257	324	413	480	2.22e-35	134
MS.gene77433.t1	MTR_6g013960	78.295	258	54	1	1	256	1	258	1.14e-144	417
MS.gene77433.t1	MTR_6g013960	83.824	68	11	0	257	324	415	482	9.01e-30	118
MS.gene77433.t1	MTR_6g011340	75.969	258	60	1	1	256	1	258	3.74e-138	400
MS.gene77433.t1	MTR_6g011340	88.235	68	8	0	257	324	415	482	1.29e-31	124
MS.gene77433.t1	MTR_6g014187	72.481	258	69	1	1	256	1	258	4.60e-134	390
MS.gene77433.t1	MTR_6g014187	80.882	68	13	0	257	324	415	482	2.23e-27	112
MS.gene77433.t1	MTR_6g014300	66.154	260	84	2	1	256	1	260	3.25e-121	357
MS.gene77433.t1	MTR_6g014300	74.627	67	17	0	258	324	402	468	2.08e-24	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77433.t1	AT1G22360	47.079	291	133	6	1	276	1	285	6.10e-82	256
MS.gene77433.t1	AT1G22360	50.000	68	33	1	257	323	412	479	7.91e-12	66.2
MS.gene77433.t1	AT1G22360	47.079	291	133	6	1	276	1	285	1.13e-81	255
MS.gene77433.t1	AT1G22400	47.203	286	129	6	7	276	10	289	7.58e-81	254
MS.gene77433.t1	AT1G22400	52.941	68	31	1	257	323	416	483	9.30e-14	72.0
MS.gene77433.t1	AT1G22340	46.316	285	132	5	7	276	10	288	6.78e-80	251
MS.gene77433.t1	AT1G22340	55.072	69	30	1	257	324	416	484	8.59e-15	75.5
MS.gene77433.t1	AT1G22370	47.018	285	125	6	7	276	10	283	9.41e-78	246
MS.gene77433.t1	AT1G22380	44.056	286	139	5	6	276	9	288	7.72e-74	236
MS.gene77433.t1	AT1G22380	52.941	68	31	1	257	323	415	482	1.12e-14	75.1

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Find 61 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGTTAAACCATCAA+AGG	0.541491	5.1:-30670684	None:intergenic
AAACGATTGCTCAAATCAAG+AGG	0.613843	5.1:+30670653	MS.gene77433:CDS
AAACTAAACAAGTAACTGGA+GGG	0.637432	5.1:-30670865	None:intergenic
AATGGAGTTAACACTGCATG+TGG	0.683935	5.1:-30670533	None:intergenic
AATGGTGTGCTACCACTCAA+AGG	0.604381	5.1:+30671010	MS.gene77433:CDS
ACATAATGATTACCTTTGAG+TGG	0.621915	5.1:-30671022	None:intergenic
ACATCTCCATCACCATCCGT+CGG	0.665024	5.1:-30670737	None:intergenic
ACCATCTGGAATAGTCTCAA+AGG	0.560749	5.1:-30670708	None:intergenic
ACCTTTGAGACTATTCCAGA+TGG	0.577669	5.1:+30670707	MS.gene77433:CDS
ACTGATTAATGAATTGATGG+TGG	0.584937	5.1:+30671892	MS.gene77433:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTAAACAAGTAACTGGA+GGG	-	chr5.1:30670868-30670887	None:intergenic	30.0%
AAGATACTAAATGTCTTGAG+TGG	+	chr5.1:30671441-30671460	MS.gene77433:intron	30.0%
ACATAATGATTACCTTTGAG+TGG	-	chr5.1:30671025-30671044	None:intergenic	30.0%
ACCTCTCTTATGAAGAAAAA+GGG	-	chr5.1:30671049-30671068	None:intergenic	30.0%
ACTGATTAATGAATTGATGG+TGG	+	chr5.1:30671892-30671911	MS.gene77433:CDS	30.0%
AGGATATTCCTGATATAATG+AGG	+	chr5.1:30671198-30671217	MS.gene77433:CDS	30.0%
ATCAATCCAATGTTGAAACT+AGG	+	chr5.1:30670572-30670591	MS.gene77433:CDS	30.0%
CATCTTTAGACTCTAATCTT+TGG	+	chr5.1:30671414-30671433	MS.gene77433:intron	30.0%
CGCAAATAATTCTACTAGTA+AGG	-	chr5.1:30671814-30671833	None:intergenic	30.0%
GTAGAATTATTTGCGAAGAA+TGG	+	chr5.1:30671819-30671838	MS.gene77433:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	30670509	30672045	30670509	ID=MS.gene77433
chr5.1	mRNA	30670509	30672045	30670509	ID=MS.gene77433.t1;Parent=MS.gene77433
chr5.1	exon	30670509	30671031	30670509	ID=MS.gene77433.t1.exon1;Parent=MS.gene77433.t1
chr5.1	CDS	30670509	30671031	30670509	ID=cds.MS.gene77433.t1;Parent=MS.gene77433.t1
chr5.1	exon	30671123	30671368	30671123	ID=MS.gene77433.t1.exon2;Parent=MS.gene77433.t1
chr5.1	CDS	30671123	30671368	30671123	ID=cds.MS.gene77433.t1;Parent=MS.gene77433.t1
chr5.1	exon	30671837	30672045	30671837	ID=MS.gene77433.t1.exon3;Parent=MS.gene77433.t1
chr5.1	CDS	30671837	30672045	30671837	ID=cds.MS.gene77433.t1;Parent=MS.gene77433.t1

Gene Sequence

>MS.gene77433

ATGAGTGACTATGCAGAAAAAAAACCACATGCAGTGTTAACTCCATTTCCAGTTCAAGGCCATATCAATCCAATGTTGAAACTAGGAAAACTACTTCACCTTAGAGGCTTTCAAATAACCTTTGTTAACACTGAATACAATCACAAACGATTGCTCAAATCAAGAGGTCCAAATGCCTTTGATGGTTTAACTGATTTTACCTTTGAGACTATTCCAGATGGTTTAACTCCGACGGATGGTGATGGAGATGTTAGTCAAGACTTACGCGCTCTTTGTCTATCAATCATGAAGAATTTTCACCACTTCTTTGGTGAATTTCTTGCTAAACTTCATGACTCTGCCACTGCTGGTCTTATCCCTCCAGTTACTTGTTTAGTTTCTGATTACAACATGCCATTTACAGTAGATGCTGCTGAAGAACATGAACTTCCAATTGTTCTCTTTTCCCCATGTAGTGCATGTTACTTCTATTCTACTTTTCATATTTCTAAATTGTTTAAGAATGGTGTGCTACCACTCAAAGGTAATCATTATGTTCCCTTTTTCTTCATAAGAGAGGTATGTATTTATGTATATAACTTCAAGAAATTGACTTTTGCAATTGTGATATTCAGATGAGAGTTATCTAACAGATGGATATTTGGACGCTAAAGTTGACGCGATTCCAGGTTTGAAAAACATTAGTTTGAAGGATATTCCTGATATAATGAGGATAAAAGATCCTGATTTGATAAAATATAAAAGTCAAGAGACAGATAAATGTCATAGAGCCTCAGCCATTGTTTTCAATACTTCTAATGAACTCGAGAGTGATGCTATGAATGCTCTTAATTCTATATTTCCATCTCTATACACCATTGGTCCATTAGCTTCTTTTGTAAATCAAAGTCCACAAAATCACTTGACATCTTTAGACTCTAATCTTTGGAAAGAAGATACTAAATGTCTTGAGTGGCTTGAATCGAAAGAACCAAAATCTGTTGTTTATGTAAATTTTGGCAGCATTACTGTTATGTCTCAAGAGAAATTGTTAGAGTTTGCTTGGGGTTTGGCGAACAGCAAGAAACCTTTTTTGTGGATTATTAGGCCTGATCTTGTTATTGGTGGCTCAGAGGTGTTGTCATCTGATTTTTTGAAAGAAATTTCAGATAGAGGCCTAATAGCAAGTTGGTGTCCACAAGAGAAAGTTTTGAACCACCCTTCAATCAGTGGATTCTTGACTCATTGCGGATGGAACTCAACCACTGAAAGTGTATGTGCTGGAGTGCCAATGTTGTGTTGGCCATTTTTTGCTGACCAACCCCTTACTAGTAGAATTATTTGCGAAGAATGGGAGATTGGGATGAAAATTGATACCAATGTAAAGAGAGAGGAGGTAGAGAAACTGATTAATGAATTGATGGTGGGAGAGAAAGGAAAGAAGATGAGGCAAAAGGCCACGGAGTTGAAGAAGAAGGCAGAGGAAGACACAAGACTCGGTGGTAGTTCATACATGAACTTGGACAAAGTGATTAAGGATGTCTTGCTTAAAATGTGA

Protein sequence

>MS.gene77433.t1

MSDYAEKKPHAVLTPFPVQGHINPMLKLGKLLHLRGFQITFVNTEYNHKRLLKSRGPNAFDGLTDFTFETIPDGLTPTDGDGDVSQDLRALCLSIMKNFHHFFGEFLAKLHDSATAGLIPPVTCLVSDYNMPFTVDAAEEHELPIVLFSPCSACYFYSTFHISKLFKNGVLPLKDESYLTDGYLDAKVDAIPGLKNISLKDIPDIMRIKDPDLIKYKSQETDKCHRASAIVFNTSNELESDAMNALNSIFPSLYTIEWEIGMKIDTNVKREEVEKLINELMVGEKGKKMRQKATELKKKAEEDTRLGGSSYMNLDKVIKDVLLKM